CN108239617A - A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof - Google Patents
A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof Download PDFInfo
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- CN108239617A CN108239617A CN201810137283.2A CN201810137283A CN108239617A CN 108239617 A CN108239617 A CN 108239617A CN 201810137283 A CN201810137283 A CN 201810137283A CN 108239617 A CN108239617 A CN 108239617A
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- paecilomyces lilacinus
- bacteria liquid
- composite bacteria
- bacillus
- rihizobium japonicum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention provides a kind of Paecilomyces lilacinus composite bacteria liquid, including following components：Bacillus, Paecilomyces lilacinus and rihizobium japonicum；The deposit number of the Paecilomyces lilacinus is CCMCC No.8450；The deposit number of the rihizobium japonicum is CGMCC 1.2550；The deposit number of the bacillus is CGMCC 1.3358.Wherein, the viable count of the bacillus, Paecilomyces lilacinus and rihizobium japonicum is respectively 108~109CFU/g.By the optimization to culture medium and condition of culture, the present invention adds in bacillus and forms composite flora the present invention using Paecilomyces lilacinus and rihizobium japonicum as main component.Three kinds of bacterium mixing, after concentrating centrifugation, composite bacteria liquid is made into nutrient solution.The effects that composite bacteria liquid can adjust soil Bacterial community, and Paecilomyces lilacinus can mitigate the harm of the nemas such as various crop root-knot nematode, cyst nematode, Ditylenchus dipsaci, and composite bacteria liquid can play nitrogen fixation and kill germ in addition.
Description
Technical field
The invention belongs to field of biological, and in particular to a kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof.
Background technology
Paecilomyces lilacinus belongs to endoparasitism fungi, is the Important Natural Enemy of some plant nematodes, can parasitize ovum,
Also larva and female adult can be infected, can the nemas such as substantially reduced various crop root-knot nematode, cyst nematode, Ditylenchus dipsaci danger
Evil.
Rhizobium are a kind of gramnegative bacteriums being distributed widely in soil, infect legume root or stem
Root nodule or stem knurl are formed afterwards, and the N in air is fixed in the form of homobium2The NH utilized for plant available4 +.Legume and root
Knurl bacterium syntaxial system has the advantages that nitrogen fixing capacity is strong, amount of nitrogen fixation is big, high-output stress-resistance, is the highest body of efficiency in biological nitrogen fixation
System, amount of nitrogen fixation account for about the 65% of biological nitrogen fixation total amount.The use of chemical fertilizer can be reduced using biological nitrogen fixation, is reduced environmental pollution
Deng.
Bacillus subtilis, a section for bacterium, can be formed gemma (endospore) bacillus or coccus it is harmful to the external world because
Sub- resistance is strong, and distribution is wide, is present in soil, water, air and animal intestinal tract etc..Adjustable flora imbalance reaches treatment mesh
, body can be promoted to generate antibacterial substance, kill pathogenic bacteria.Resistant activity material can be generated, and is taken by force with unique biology
Oxygen mechanism of action can inhibit the growth and breeding of pathogenic bacteria.
Agron is that organic matter converts the colloidal substance to be formed, generally black or burgundy through microorganism decomposition,
It is the chief component of the soil organism.Currently, chemical fertilizer, excessive cultivation, single farming are used due to unreasonable
Soil degradation problem caused by system serious threat to agricultural sustainable development, wherein, humus in soil is reduced
It is major reason.In addition, the harm of the nemas such as nematode, cyst nematode, Ditylenchus dipsaci also seriously affects the yield of crop.
Invention content
In view of this, the purpose of the present invention is to provide a kind of Paecilomyces lilacinus composite bacteria liquid, including following components：Gemma
Bacillus, Paecilomyces lilacinus and rihizobium japonicum.
Preferably, in Paecilomyces lilacinus composite bacteria liquid of the present invention, the Paecilomyces lilacinus is commonly used in the art
Paecilomyces lilacinus；It is highly preferred that the deposit number of the Paecilomyces lilacinus is CCMCCNo.8450.
Preferably, in Paecilomyces lilacinus composite bacteria liquid of the present invention, the rihizobium japonicum is commonly used in the art
Rihizobium japonicum；It is highly preferred that the deposit number of the rihizobium japonicum is CGMCC1.2550.
Preferably, in Paecilomyces lilacinus composite bacteria liquid of the present invention, the bacillus is commonly used in the art
Bacillus；It is highly preferred that the deposit number of the bacillus is CGMCC 1.3358.
Preferably, in Paecilomyces lilacinus composite bacteria liquid of the present invention, the bacillus, Paecilomyces lilacinus and big
The viable count of beans rhizobium is respectively 108~109CFU/g。
Another aspect of the invention is the preparation methods for providing Paecilomyces lilacinus composite bacteria liquid, include the following steps：
1) fluid nutrient medium culture is accessed after Paecilomyces lilacinus strain is activated；
2) rihizobium japonicum is activated, accesses fluid nutrient medium culture；
3) bacillus living is dissolved in aqueous solution；
4) merge the bacterium solution of step 1)~step 3), mixed bacteria liquid concentration centrifugation；
5) mixing is concentrated into centrifugation bacterium solution and adds in nutrient solution to get Paecilomyces lilacinus composite bacteria liquid.
Preferably, in the preparation method of Paecilomyces lilacinus composite bacteria liquid of the present invention, the guarantor of the Paecilomyces lilacinus
It is CCMCCNo.8450 to hide number；The deposit number of the rihizobium japonicum is CGMCC1.2550；The preservation of the bacillus
Number is CGMCC 1.3358.
Preferably, in the preparation method of Paecilomyces lilacinus composite bacteria liquid of the present invention, the step 1)~step 3)
The viable count of middle Paecilomyces lilacinus strain, rihizobium japonicum and bacillus living powder is respectively 108~109CFU/g；The step
It is rapid 5) in l mixing concentration centrifugation bacterium solution and nutrient solution ratio be 2:1 volume ratio；Preferably, nutrient solution of the present invention is plants
Object growth nutrient solution, more preferably commercially available Aron-Hoagland nutrient solutions；Heretofore described Paecilomyces lilacinus is compound
The volumn concentration of nutrient solution is preferably 45~55%, more preferably 50% in bacterium solution.
Preferably, in the preparation method of Paecilomyces lilacinus composite bacteria liquid of the present invention, culture item in the step 1)
Part is 35~39 DEG C, and 120~130rpm cultivates 15~20h.
Preferably, in the preparation method of Paecilomyces lilacinus composite bacteria liquid of the present invention, culture item in the step 2)
Part is 35~39 DEG C, and 120~130rpm cultivates 15~20h.
Compared with prior art, the present invention the present invention has the following advantages：
The present invention by the optimization to culture medium and condition of culture, the present invention using Paecilomyces lilacinus and rihizobium japonicum as
Main component, and add in bacillus and form composite flora.Three kinds of bacterium mixing, after concentrating centrifugation, are made into nutrient solution
Composite bacteria liquid.Composite bacteria liquid can adjust soil Bacterial community, and Paecilomyces lilacinus can mitigate various crop root-knot nematode, cyst
The effects that harm of the nemas such as nematode, Ditylenchus dipsaci, composite bacteria liquid can play nitrogen fixation and kill germ in addition.
Present inventors have unexpectedly found that ecological flora is had adjusted in soil by the cooperation of three kinds of floras so that composite bacteria liquid can be with
Promote microorganism decomposition organic matter, form humus, by improving the physicochemical property of soil, break soil hardening and compacting, promote
Into the decomposition of the soil organism and the release of nutritional ingredient, soil control fertilizer ability is improved, adjusts soil pH, adjusts microorganism species
Structure inhibits harmful microbe growth, enhances root system of plant to the absorbability of nutriment and promote growth, reach and change
Good soil effect, and then with the abundant metabolin of soil protection, the decomposition of promotion organic matter, generation and inhibit the works such as harmful bacteria
With so as to play the growth and protective effect to plant, the absorption and growth of promotion Plant To Nutrient substance.Pass through subsequent implementation
The composite bacteria liquid of the bright present invention of illustration has good volume increase effect of gain.
Specific embodiment
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the reality in the present invention
Apply example, those of ordinary skill in the art's all other embodiments obtained without making creative work all belong to
In the scope of protection of the invention.
Bacterial strain is bacterial strain commonly used in the art in the present embodiment, and city is available；The pale purple plan described in the embodiment of the present invention 1
The deposit number of mould is CCMCC No.8450；The deposit number of the rihizobium japonicum is CGMCC 1.2550；The gemma
The deposit number of bacillus is CGMCC 1.3358.
Embodiment 1
1) Paecilomyces lilacinus culture：The Paecilomyces lilacinus strain that 2 rings is taken to activate, access loading amount are 100mL fluid nutrient mediums
500mL triangular flasks in, 30 DEG C, 126rpm culture 18h.
2) rihizobium japonicum is cultivated：The strain of 2 rings activation rihizobium japonicum is taken, access loading amount is 100mL fluid nutrient mediums
500mL triangular flasks in, 37 DEG C, 126rpm culture 18h.
3) bacillus bacterium solution：5g commercialization bacillus powder is taken to be dissolved in 100ml aqueous solutions.
4) above-mentioned three steps bacterium solution is merged, obtains 300ml mixed bacteria liquids, 200ml bacterium solutions, the gemma are centrifuged by concentration
The viable count of bacillus, Paecilomyces lilacinus and rihizobium japonicum is respectively 108~109CFU/g。
5) 200ml is mixed into concentration centrifugation bacterium solution and presses 2:1 volume ratio adds in 50% volume Aron-Hoagland nutrient solutions,
Up to 300ml Paecilomyces lilacinus composite bacteria liquids.
Embodiment 2
The Paecilomyces lilacinus composite bacteria liquid that embodiment 1 is obtained, (deposit number is with Paecilomyces lilacinus
CCMCCNo.8450), rihizobium japonicum (deposit number is CGMCC 1.2550), bacillus (deposit number CGMCC
1.3358) independent bacterium solution (identical viable count) is compared, and finds to increase by 19% to agron after handling soil, so as to reach
Improve the effect of soil.
Found by cultivating same breed potato, bacillus subtilis that the present invention obtains, bacillus licheniformis with it is double
The composite bacteria liquid of discrimination bacillus, with 3 kinds of bacillus subtilises, bacillus licheniformis compared with the independent bacterium solution of Bifidobacterium, Ma Ling
Potato weightening 25%.
It is found by cultivating same breed wheat, bacillus subtilis, bacillus licheniformis and the bifid that the present invention obtains
The composite bacteria liquid of bacillus is small with 3 kinds of bacillus subtilises, bacillus licheniformis compared with arbitrary two kinds of bacterium solutions of Bifidobacterium
More than 15% wheat weightening.
Found by cultivating same breed tomato, bacillus subtilis that the present invention obtains, bacillus licheniformis with it is double
The composite bacteria liquid of discrimination bacillus, compared with commercially available farm manure, weightening 18%.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Paecilomyces lilacinus composite bacteria liquid, including following components：Bacillus, Paecilomyces lilacinus and rihizobium japonicum.
2. Paecilomyces lilacinus composite bacteria liquid according to claim 1, which is characterized in that the preservation of the Paecilomyces lilacinus is compiled
Number be CCMCC No.8450.
3. Paecilomyces lilacinus composite bacteria liquid according to claim 1, which is characterized in that the preservation of the rihizobium japonicum is compiled
Number be CGMCC 1.2550.
4. Paecilomyces lilacinus composite bacteria liquid according to claim 1, which is characterized in that the deposit number of the bacillus
For CGMCC 1.3358.
5. Paecilomyces lilacinus composite bacteria liquid according to claims 2 to 4, which is characterized in that the bacillus, pale purple plan
Mould and the viable count of rihizobium japonicum are respectively 108~109CFU/g。
6. a kind of preparation method of Paecilomyces lilacinus composite bacteria liquid, includes the following steps：
1) commercial Paecilomyces lilacinus strain is taken, fluid nutrient medium culture is accessed after activation；
2) after rihizobium japonicum is activated, fluid nutrient medium culture is accessed；
3) bacillus living is dissolved in aqueous solution；
4) merge the bacterium solution of step 1)~step 3), mixed bacteria liquid concentration centrifugation；
5) mixing is concentrated into centrifugation bacterium solution and adds in nutrient solution to get Paecilomyces lilacinus composite bacteria liquid.
7. the preparation method of Paecilomyces lilacinus composite bacteria liquid according to claim 6, which is characterized in that the pale purple plan is green
Mould deposit number is CCMCC No.8450；The deposit number of the rihizobium japonicum is CGMCC 1.2550；The gemma bar
The deposit number of bacterium is CGMCC 1.3358.
8. the preparation method of Paecilomyces lilacinus composite bacteria liquid according to claim 7, which is characterized in that the step 1)~
The viable count of Paecilomyces lilacinus strain, rihizobium japonicum and bacillus living powder is respectively 10 in step 3)8~109CFU/g；
L mixing concentration centrifugation bacterium solution and the ratio of nutrient solution are 2 in the step 5):1 volume ratio.
9. the preparation method of Paecilomyces lilacinus composite bacteria liquid according to claim 7, which is characterized in that in the step 1)
Condition of culture is 35~39 DEG C, and 120~130rpm cultivates 15~20h.
10. the preparation method of Paecilomyces lilacinus composite bacteria liquid according to claim 7, which is characterized in that the step 2)
Middle condition of culture is 35~39 DEG C, and 120~130rpm cultivates 15~20h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810137283.2A CN108239617A (en) | 2018-02-10 | 2018-02-10 | A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof |
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| CN201810137283.2A CN108239617A (en) | 2018-02-10 | 2018-02-10 | A kind of Paecilomyces lilacinus composite bacteria liquid and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212722A (en) * | 2013-12-12 | 2014-12-17 | 谢明 | Paecilomyces lilacinus, culture method of paecilomyces lilacinus and use of paecilomyces lilacinus in prevention and control of diseases and pests of crops |
| CN105567769A (en) * | 2016-01-26 | 2016-05-11 | 湖北工业大学 | Synergist for bacillus subtilis capable of generating antibacterial peptides through fermentation and use method of synergist |
| CN106977344A (en) * | 2016-12-26 | 2017-07-25 | 山东美吉多生态农业科技有限公司 | A kind of mulch biological organic fertilizer and preparation method thereof |
| CN107043727A (en) * | 2017-06-15 | 2017-08-15 | 安徽瑞驰兰德生物科技有限公司 | One kind makes fertilizer and urges rotten type composite bacteria agent and its preparation method and application |
-
2018
- 2018-02-10 CN CN201810137283.2A patent/CN108239617A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104212722A (en) * | 2013-12-12 | 2014-12-17 | 谢明 | Paecilomyces lilacinus, culture method of paecilomyces lilacinus and use of paecilomyces lilacinus in prevention and control of diseases and pests of crops |
| CN105567769A (en) * | 2016-01-26 | 2016-05-11 | 湖北工业大学 | Synergist for bacillus subtilis capable of generating antibacterial peptides through fermentation and use method of synergist |
| CN106977344A (en) * | 2016-12-26 | 2017-07-25 | 山东美吉多生态农业科技有限公司 | A kind of mulch biological organic fertilizer and preparation method thereof |
| CN107043727A (en) * | 2017-06-15 | 2017-08-15 | 安徽瑞驰兰德生物科技有限公司 | One kind makes fertilizer and urges rotten type composite bacteria agent and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| CASTILLO JD ET AL: "Biocontrol of the reniform nematode by Bacillus firmus GB-126 and Paecilomyces lilacinus 251 on Cotton", 《PLANT DIS》 * |
| 张雪艳 等: "单一或组合生防菌对田间番茄植株生长和线虫的影响", 《北方园艺》 * |
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Application publication date: 20180703 |