CN108617696A - A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen and preparation method thereof and the Citrus Huanglongbing pathogen special biological agent prepared with it - Google Patents
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen and preparation method thereof and the Citrus Huanglongbing pathogen special biological agent prepared with it Download PDFInfo
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- CN108617696A CN108617696A CN201810403083.7A CN201810403083A CN108617696A CN 108617696 A CN108617696 A CN 108617696A CN 201810403083 A CN201810403083 A CN 201810403083A CN 108617696 A CN108617696 A CN 108617696A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M7/00—Special adaptations or arrangements of liquid-spraying apparatus for purposes covered by this subclass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The Citrus Huanglongbing pathogen special biological agent prepared the present invention relates to agricultural plantation technology field more particularly to a kind of complex micro organism fungicide for Citrus Huanglongbing pathogen and preparation method thereof and with it.The present invention controls former ground Citrus Huanglongbing pathogen and killing propagating source wood louse and its worm's ovum to reach the generation of effectively control yellow twig by bacillus thuringiensis, beauveria bassiana, trichoderma harzianum, four kinds of microbial bacterias of under ground portion effectively control the infringement of pathogenic microorganism, effectively propagation of the control nematode to pathogen, adjust soil texture simultaneously, organic matter is decomposed, is increase soil fertility；Promote the growth of citrus root by the obligate AM fungies of citrus, citrus is set to grow fasciation new root, expand new root quantity, enhance tree vigo(u)r, improve the immune disease-resistance ability of tree, Nitrogen Fixation System is formed, the ecological environment for controlling cause of disease and improvement Ci trus growth is combined together, achievees the effect that prevent and treat Citrus Huanglongbing pathogen.
Description
Technical field
The present invention relates to agricultural plantation technology field more particularly to a kind of complex micro organism fungicides for Citrus Huanglongbing pathogen
And preparation method thereof and with its preparation Citrus Huanglongbing pathogen special biological agent.
Background technology
Currently, with the variation of the environmental conditions such as weather, soil, human factor, it is more next that citrus plants problem encountered
It is more.Citrus pest and disease damage aggravates, and especially Citrus Huanglongbing pathogen is increasingly severe, the glutinous rehmannia such as Guangdong, Guangxi, Jiangxi, Hunan, Fujian
Dragon is critically ill, and evil is serious, and large quantities of orchard yellows lose production capacity；Eelworm harm aggravates year by year, has seriously affected the yield of citrus.
All kinds of pest and disease damages of citrus cause huge economic loss to Citrus Industry.
Citrus Huanglongbing pathogen is caused by a kind of gramnegative bacterium being limited to bast endoparasitism, can infect including
A variety of rutaceaes such as Citrus, Poncirus, Fortunella and kamuning.Citrus Huanglongbing pathogen is the destructiveness on world's Orange Producing
Disease, the disease are mainly distributed on nearly 50 countries and regions in Asia, Africa, Oceania, South America and North America, 19, China
Orange Producing, which saves in (city, autonomous region), has 11 by the evil of being critically ill, the serious sound development for restricting Citrus Industry.
The patent majority of existing treatment Citrus Huanglongbing pathogen utilizes single microorganism, as patent 201110332473.8 (utilizes
Muscardine controls the purposes and method of citrus psylla) a kind of method that wood louse is controlled by muscardine is disclosed, only from surface
On control the communication media of Citrus Huanglongbing pathogen, the threat without solving citrus soil-borne disease is not controlled effectively fundamentally
Treat Citrus Huanglongbing pathogen；Such as a kind of (the Trichoderma harzianum class complex bacteria culture and its in terms of plant protection of patent 200910016337.0
Using), a kind of Trichoderma harzianum class complex bacteria culture and its application in terms of plant protection are disclosed, resistance various plants have been reached
The effect that disease threatens, but specific aim is not strong, and the communication media wood louse of Citrus Huanglongbing pathogen could not effectively be controlled, it cannot be effective
Control the disease propagation；As patent 201610745978.X (it is a kind of prevention cumquat yellow twig microbial bacterial agent and its preparation
Method), a kind of microbial bacterial agent and preparation method thereof of prevention cumquat yellow twig is disclosed, which is by Gu Lake
Streptomycete, Ka Wuer streptomycetes, the sugared bacillus of cold solution, Nissl bacillus mix in proportion after the fermentation of the bacterium such as tuft trichoderma
It forms, the control from desinsection to soil-borne disease, all efficiently solves the control of Citrus Huanglongbing pathogen, and it has rhizosphere to citrus
Growth-promoting functions, but it is not few from citrus root hair, and group planting mycorhiza improves anti pathologic immunity ability, citrus from the point of view of being formed less to citrus
Although being likely to be obtained Morbidity control, Citrus Huanglongbing pathogen influences the whole tree vigo(u)r of citrus big, no good root system system, cannot be light
Easily restore tree vigo(u)r, it is not notable to restore life effect to citrus orchard.
Invention content
In order to solve the above technical problems existing in the prior art, the present invention provides a kind of answering for Citrus Huanglongbing pathogen
Microbial bacterial agent and preparation method thereof and the Citrus Huanglongbing pathogen special biological agent with its preparation are closed, particular by following technology
What scheme was achieved：
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen by bacillus thuringiensis, beauveria bassiana, breathes out thatch wood
Mould, Frankia compounds.The bacillus thuringiensis, beauveria bassiana, Trichoderma harzianum, Forlan kirschner unwrapping wire
The mass ratio of bacterium is (1.5-2.5):(2.5-3.5):(3-5):(0.5-1.5).
Preferably, the bacillus thuringiensis, beauveria bassiana, Trichoderma harzianum, Frankia mass ratio
It is 2:3:4:1.
The bacillus thuringiensis, is prepared via a method which：Bacillus thuringiensis strain is cultivated in LB
Culture is expanded numerous in base, is then seeded into solid medium, inoculum concentration 6%, under 30-32 DEG C of PH7.2, temperature environment, culture
36-72 hours, the solid medium：Solid constituent is wheat bran 63%, soya-bean cake 18%, corn flour 10%, molasses 5%, yeast
Powder 1%, sodium glutamate 0.4%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.3%, sulfuric acid diammonium 1.2%, magnesium sulfate 0.6%,
Water content is 60%.
The beauveria bassiana, is prepared via a method which：The beauveria bassiana bought is cultivated in the lab
Expand numerous, be sprayed on and suffer from the serious tangerine garden of Citrus Huanglongbing pathogen, spends 3-5 days and collect the wood louse infected by beauveria bassiana, extract wherein
Beauveria bassiana, carry out laboratory and be separately cultured, by inoculation carry out solid expand it is numerous, after so that it is generated a large amount of conidiums,
Beauveria bassiana pulvis is made.
The Trichoderma harzianum, is prepared via a method which：Trichoderma harzianum strain T-22 is passed through into solid PDA medium
28 DEG C of culture is cultivated 2-3 days, it is detected to the common gray mold of plant, anthracnose, powdery mildew, downy mildew by pathology cultivation
Deng disease resistance, select the strongest bacterial strain of comprehensive disease resistance and cultivated, pass through existing mature technology carry out inclined-plane culture, eggplant
It is transferred to solid spawn culture after bottle culture, strain cultivation, Trichoderma harzianum pulvis is made.
The Frankia, is prepared via a method which：First planted with Gause I synthetic media
Son culture, obtains solid seed culture medium, and sporozoites inoculation is carried out on fermentation material, frankia is obtained after fermented and cultured
Microbial inoculum raw material obtains frankia bacterium powder by processing.
Bacillus thuringiensis：That is Dipel, abbreviation Bt are a kind of production crystal gemma bars for including many mutation
Bacterium.The bacterium can generate two major classes toxin, i.e. endotoxin (parasporal crystal) and exotoxin, make pest stop feeding, last pest because
It is hungry and be poisoned to death.Therefore the bacillus can do microbial source low toxic pesticide, for prevent Orthoptera, coleoptera, Diptera,
The various pests of Hymenoptera, especially Lepidoptera.
Beauveria bassiana：Belong to Ascomycota, Hypocreales, cordyceps sinensis Cordycepps, Beauveria, is that broad spectrum activity Insect Pathogenic is true
Bacterium is substantially carried out vegetative propagation, generates conidium；It can carry out zoogamy and generate fructification, be accredited as ball spore cordyceps sinensis.
Beauveria bassiana is used for biological control of insect pests in world wide, wherein China using beauveria bassiana Control Dendrolimus punctatus and
Corn borer is maximum control of insect project in the world, obtains good pest and continues control effect.It is that broad spectrum insecticide is true
Bacterium, domestic and international researcher utilize beauveria bassiana prevention corn borer, pine moth, small sugarcane borer, blind Chinese toon, grain weevil, citrus red mite
With the agriculture and forestry injurious insects such as aphid.Especially to the biological control of corn borer and pine moth, it has been used as conventional means to make year after year at home
With.Since beauveria bassiana can efficiently control population number, while other natural enemy insects and beneficial organism are not injured, accord with completely
Close the objective of integrated pest management.
Trichoderma harzianum：Trichoderma harzianum is one kind of trichoderma, belongs to Fungi Imperfecti door, hyphomycetales, trichoderma.Trichoderma harzianum T-
22, can be used for preventing by pythium spp as a kind of biocontrol microorganisms, Rhizoctonia solani Kuhn, sickle-like bacteria, bread mold, column spore are mould, sclerotinite,
Plant disease caused by the pathogens such as Sclerotium rolfsii.Its principle active component is trichoderma harzianum T-22 strains, and Trichoderma is
A kind of microorganism being widely present in nature, trichoderma harzianum are the strain applied in Trichoderma, trichoderma harzianum
T-22 strains are manually modified strains, are that be that male parent is obtained by cell-fusion techniques by T95 strains and T12 strains artificial
Hybridize strain.T95 strains are strong to the winding ability and colonization ability of root system of plant, and T12 strains are strong to the prevention and control capability of disease, lead to
It crosses cell-fusion techniques to combine the advantages of both, to obtain warped roots, field planting, disease prevention and control ability all
Excellent strain T22, while adaptability of its male parent to soil type is obtained, it can be in sandy loam and clayed soil
Good field planting breeding, makes the application of T22 have more adaptability.
Frankia：Frankia right and wrong legume symbiosis formation root is lame and can fix the unwrapping wire of atmospheric nitrogen
Bacterium.Gram-positive is indefinite.Probably it is micro aerobic mesophile.It is developed in the tissue in-growth of aulophyte；It may also
It is free to be present in soil, probably it is rest stage.Appropriate host plant is met, by root hair into people's root system of plant, is generated simultaneously
Auxin makes Root hair curling, endophyte reach the cortex of root by infection line, causes the proliferation of lateral root meristem cell, forms root
Tumor.Root nodule is exactly the deformation lateral root that growth is suppressed.The cell of infection expands, and has the big core of deformation.
The present invention by bacillus thuringiensis, beauveria bassiana, trichoderma harzianum control ground Citrus Huanglongbing pathogen it is former and
Propagating source wood louse and its worm's ovum are killed to reach the generation of effectively control yellow twig, four kinds of microbial bacterias of under ground portion are effective
The infringement of pathogenic microorganism is controlled, propagation of the nematode to pathogen is effectively controlled, while adjusting soil texture, decomposes organic matter,
It increases soil fertility.
A series of research and experiment are carried out in process of the present invention, following selected part process is disclosed：
1：Microorganism proportioning test
Test example 1：Screening test is carried out to the proportioning of microorganism：
Numerous good bacillus thuringiensis, beauveria bassiana, Trichoderma harzianum, Frankia will be expanded by following each matter
Amount is more for use than being dispensed after being mixed.
Citrus orchard with yellow twig is uniformly divided into 14 equal-sized plot, between mutually completely cut off, apply respectively
With the microorganism formulation of 1-13 groups, last group is not applied as blank control group, counts citrus in each plot within 2 months after
Wood louse bacterial bearing rate, diaphorina citri effectively " strain worm amount ", citrus disease index, control effect.
By upper data it is found that by bacillus thuringiensis, beauveria bassiana, Trichoderma harzianum, Frankia according to
Suitable ratio mixing, can significantly reduce diaphorina citri bacterial bearing rate, diaphorina citri effectively " strain worm amount ", fully improve citrus disease
Feelings index obtains preferable Citrus Huanglongbing pathogen control effect.
A kind of Citrus Huanglongbing pathogen special biological agent, including it is previously described for the composite microbial bacteria of Citrus Huanglongbing pathogen
Agent, microbial adsorbent, the obligate AM Inoculants of citrus, and moisture are 15-20%.
The obligate AM fungies Inoculant of citrus can promote the growth of citrus root, and citrus is made to grow fasciation new root, expand new
Radical amount, effectively enhances tree vigo(u)r, improves the immune disease-resistance ability of tree, forms Nitrogen Fixation System, increases soil fertility, increases Tiny ecosystem
The sources of citrus intake nutrient in environment.
A kind of preparation method preparing the Citrus Huanglongbing pathogen special biological agent, includes the following steps：
(1) microbial adsorbent is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of citrus；
(3) adjust mixed preparation moisture to 15-20% to get.
The application method of the Citrus Huanglongbing pathogen special biological agent, includes the following steps：
A, by biological agent according to 1:The weight ratio of 500-800 is diluted with water, and stands 5-20 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble bottom
Object, for irrigating tree root.
The Citrus Huanglongbing pathogen special biological agent of the present invention is by bacillus thuringiensis, beauveria bassiana, breathes out thatch wood
Mould control ground Citrus Huanglongbing pathogen is former and kills propagating source wood louse and its worm's ovum to reach the generation of effectively control yellow twig,
Four kinds of microbial bacterias of under ground portion effectively control the infringement of pathogenic microorganism, effectively control propagation of the nematode to pathogen, together
When adjust soil texture, decompose organic matter, increase soil fertility.The obligate AM fungies Inoculant of citrus can promote citrus root
Growth makes citrus grow fasciation new root, expands new root quantity, effectively enhances tree vigo(u)r, improves the immune disease-resistance ability of tree, is formed solid
Nitrogen system increases soil fertility, increases the sources of citrus intake nutrient in micro-ecological environment.By controlling cause of disease, improve
The ecological environment of Ci trus growth, thoroughly cures Citrus Huanglongbing pathogen.
Compared with prior art, the technique effect of the invention is embodied in：
The present invention the complex micro organism fungicide for Citrus Huanglongbing pathogen, by bacillus thuringiensis, beauveria bassiana,
Trichoderma harzianum control ground Citrus Huanglongbing pathogen is former and kills propagating source wood louse and its worm's ovum to reach effectively control yellow twig
Generation, four kinds of microbial bacterias of under ground portion effectively control the infringement of pathogenic microorganism, effectively control nematode to pathogen
It propagates, while adjusting soil texture, decompose organic matter, increase soil fertility.
The Citrus Huanglongbing pathogen special biological agent of the present invention is by bacillus thuringiensis, beauveria bassiana, breathes out thatch wood
Mould control ground Citrus Huanglongbing pathogen is former and kills propagating source wood louse and its worm's ovum to reach the generation of effectively control yellow twig,
Four kinds of microbial bacterias of under ground portion effectively control the infringement of pathogenic microorganism, effectively control propagation of the nematode to pathogen, together
When adjust soil texture, decompose organic matter, increase soil fertility.The obligate AM fungies Inoculant of citrus can promote citrus root
Growth makes citrus grow fasciation new root, expands new root quantity, effectively enhances tree vigo(u)r, improves the immune disease-resistance ability of tree, is formed solid
Nitrogen system increases soil fertility, increases the sources of citrus intake nutrient in micro-ecological environment.By controlling cause of disease, improve
The ecological environment of Ci trus growth, thoroughly cures Citrus Huanglongbing pathogen.
Specific implementation mode
It is limited with reference to specific embodiment technical scheme of the present invention is further, but claimed
Range describes made by being not only limited to.
Prepare bacillus thuringiensis, beauveria bassiana, Trichoderma harzianum, Forlan kirschner unwrapping wire respectively by the following method
Bacterium, for use.
It is prepared via a method which bacillus thuringiensis：Bacillus thuringiensis strain is cultivated to expansion in LB culture mediums
It is numerous, it is then seeded into solid medium, inoculum concentration 6%, under 30-32 DEG C of PH7.2, temperature environment, cultivates 36-72 hours,
The solid medium：Solid constituent is wheat bran 63%, soya-bean cake 18%, corn flour 10%, molasses 5%, yeast powder 1%, paddy ammonia
Sour sodium 0.4%, dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.3%, sulfuric acid diammonium 1.2%, magnesium sulfate 0.6%, water content are
60%.
It is prepared via a method which beauveria bassiana：The beauveria bassiana bought is cultivated in the lab and expands numerous, sprinkling
In suffering from the serious tangerine garden of Citrus Huanglongbing pathogen, spends 3-5 days and collect the wood louse infected by beauveria bassiana, it is stiff in vain to extract ball spore therein
Bacterium carries out laboratory and is separately cultured, numerous by inoculation progress solid expansion, and after so that it is generated a large amount of conidiums, it is white that ball spore is made
Stiff bacteria powder.
It is prepared via a method which Trichoderma harzianum：Trichoderma harzianum strain T-22 is passed through into 28 DEG C of solid PDA medium culture
Culture 2-3 days, it is detected to the disease-resistant of the common gray mold of plant, anthracnose, powdery mildew, downy mildew etc. by pathology cultivation
Ability is selected the strongest bacterial strain of comprehensive disease resistance and is cultivated, cultivated by existing mature technology progress inclined-plane culture, eggplant bottle,
It is transferred to solid spawn culture after strain cultivation, Trichoderma harzianum pulvis is made.
It is prepared via a method which Frankia：Seed culture first is carried out with Gause I synthetic media, is obtained
Solid seed culture medium is obtained, sporozoites inoculation is carried out on fermentation material, frankia microbial inoculum raw material is obtained after fermented and cultured,
Frankia bacterium powder is obtained by processing.
Embodiment 1
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen, by 2kg bacillus thuringiensis, 3kg beauveria bassianas,
4kg Trichoderma harzianums, 1kg Frankias mix.
Embodiment 2
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen is white by 2.5kg bacillus thuringiensis, 2.5kg ball spores
Stiff bacterium, 3kg Trichoderma harzianums, 0.5kg Frankias mix.
Embodiment 3
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen is white by 1.5kg bacillus thuringiensis, 3.5kg ball spores
Stiff bacterium, 3kg Trichoderma harzianums, 0.5kg Frankias mix.
Embodiment 4
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen is white by 1.5kg bacillus thuringiensis, 2.5kg ball spores
Stiff bacterium, 5kg Trichoderma harzianums, 0.5kg Frankias mix.
Embodiment 5
A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen is white by 1.5kg bacillus thuringiensis, 2.5kg ball spores
Stiff bacterium, 3kg Trichoderma harzianums, 1.5kg Frankias mix.
Embodiment 6
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 50% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 25%；
(3) adjust mixed preparation moisture to 17.5% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 1 obtains.
Embodiment 7
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 10% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 5%；
(1) adjust mixed preparation moisture to 15% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 1 obtains.
Embodiment 8
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 100% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 50%；
(3) adjust mixed preparation moisture to 20% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 1 obtains.
Embodiment 9
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of citrus；
(3) adjust mixed preparation moisture to 17.5% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 2 obtains.
Embodiment 10
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 50% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 25%；
(3) adjust mixed preparation moisture to 17.5% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 3 obtains.
Embodiment 11
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 50% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 25%；
(3) adjust mixed preparation moisture to 17.5% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 4 obtains.
Embodiment 12
A kind of Citrus Huanglongbing pathogen special biological agent, is prepared via a method which：
(1) microbial adsorbent of microbial bacterial agent weight 50% is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of the citrus of microbial bacterial agent weight 25%；
(3) adjust mixed preparation moisture to 17.5% to get.
The microbial bacterial agent is the complex micro organism fungicide that embodiment 5 obtains.
Comparative example 1：It is prepared according to the embodiment 1 of patent application CN201610745978.X, method of administration is according to patent
Progress described in CN201610745978.X embodiments 2.
Comparative example 2：It is prepared according to the embodiment of patent application 201110332473.8, method of administration is according to patent application
201110332473.8 the progress described in embodiment.
Comparative example 3：Use the MDCGTH18 microbial inoculums in the embodiment 1 of patent application 200910016337.0, method of administration
It is method 4.
Citrus orchard with yellow twig is uniformly divided into 16 equal-sized plot, between mutually completely cut off, respectively with
The microbial bacterial agent of the application 1-5 groups of method 4 applies the Citrus Huanglongbing pathogen special biological agent of embodiment 6-12 in method 1, with side
The Citrus Huanglongbing pathogen special biological agent of the application embodiment 1 of method 2 applies the microbial bacterial agent of embodiment 1 in method 3, by comparison
Method in example 1 applies comparative example 1, last group is not applied as blank control group, and each plot citrus is counted within 6 months after
Disease index, control effect, current year citrus per mu yield, disease citrus ratio.
Specific method of administration is as follows：
Method 1：
A, by biological agent according to 1:650 weight ratio is diluted with water, and stands 12.5 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble bottom
Object, for irrigating tree root.
Method 2：
A, by biological agent according to 1:500 weight ratio is diluted with water, and stands 5 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble bottom
Object, for irrigating tree root.
Method 3：
A, by biological agent according to 1:800 weight ratio is diluted with water, and stands 20 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble bottom
Object, for irrigating tree root.
Method 4：
A, by microbial bacterial agent according to 1:650 weight ratio is diluted with water, and stands 12.5 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble bottom
Object, for irrigating tree root.
By upper data it is found that the present invention yellow twig specific complex microbial bacterial agent and yellow twig special biological agent,
Citrus disease index can fully be improved, obtain preferable Citrus Huanglongbing pathogen control effect, and while treating yellow twig,
It is rapid to restore tree vigo(u)r, current year per mu yield is greatly promoted, the catch an illness citrus of garden of yellow twig is enable quickly to recover economy benefit.
1-5 groups and 6,9,10,11,12 groups are compared it is found that by microbial bacterial agent and microbial adsorbent and citrus
Obligate AM Inoculants, which are mixed and made into Citrus Huanglongbing pathogen special biological agent, can effectively promote control effect to yellow twig, and fast
Quick-recovery tree vigo(u)r promotes current year per mu yield.
Finally it is pointed out that above example is only the more representational example of the present invention.Obviously, technology of the invention
Scheme is not limited to above-described embodiment, and acceptable there are many deformations.Those skilled in the art can be from disclosed by the invention
All deformations that content is directly exported or associated, are considered as protection scope of the present invention.
Claims (10)
1. a kind of complex micro organism fungicide for Citrus Huanglongbing pathogen, which is characterized in that be white by bacillus thuringiensis, ball spore
Stiff bacterium, Trichoderma harzianum, Frankia compound.
2. the complex micro organism fungicide according to claim 1 for Citrus Huanglongbing pathogen, which is characterized in that the Su Yun gold
Bacillus, beauveria bassiana, Trichoderma harzianum, Frankia mass ratio be (1.5-2.5):(2.5-3.5):(3-
5):(0.5-1.5)。
3. the complex micro organism fungicide according to claim 2 for Citrus Huanglongbing pathogen, which is characterized in that the Su Yun gold
Bacillus, beauveria bassiana, Trichoderma harzianum, Frankia mass ratio be 2:3:4:1.
4. the complex micro organism fungicide according to claim 1 for Citrus Huanglongbing pathogen, which is characterized in that the Su Yun gold
Bacillus is prepared via a method which：Bacillus thuringiensis strain is cultivated in LB culture mediums and expands numerous, is then connect
In kind to solid medium, inoculum concentration 6% is cultivated 36-72 hours under 30-32 DEG C of PH7.2, temperature environment, the solid training
Support base：Solid constituent be wheat bran 63%, soya-bean cake 18%, corn flour 10%, molasses 5%, yeast powder 1%, sodium glutamate 0.4%,
Dipotassium hydrogen phosphate 0.5%, ferrous sulfate 0.3%, sulfuric acid diammonium 1.2%, magnesium sulfate 0.6%, water content 60%.
5. the complex micro organism fungicide according to claim 1 for Citrus Huanglongbing pathogen, which is characterized in that the ball spore is white
Stiff bacterium, is prepared via a method which：It is numerous that the beauveria bassiana bought is cultivated to expansion in the lab, is sprayed on and suffers from citrus Huang
The imperial serious tangerine garden of disease, spends 3-5 days and collects the wood louse infected by beauveria bassiana, extract beauveria bassiana therein, carries out real
Room is tested to be separately cultured, it is numerous by inoculation progress solid expansion, after so that it is generated a large amount of conidiums, beauveria bassiana pulvis is made.
6. the complex micro organism fungicide according to claim 1 for Citrus Huanglongbing pathogen, which is characterized in that the Kazakhstan thatch wood
It is mould, it is prepared via a method which：Trichoderma harzianum strain T-22 is cultivated 2-3 days for 28 DEG C by solid PDA medium culture,
Its disease resistance to the common gray mold of plant, anthracnose, powdery mildew, downy mildew etc. is detected by pathology cultivation, is selected
The strongest bacterial strain of comprehensive disease resistance is cultivated, and inclined-plane culture, the culture of eggplant bottle, liquid spawn training are carried out by existing mature technology
It is transferred to solid spawn culture after supporting, Trichoderma harzianum pulvis is made.
7. the complex micro organism fungicide according to claim 1 for Citrus Huanglongbing pathogen, which is characterized in that described Frank
Family name actinomyces, are prepared via a method which：Seed culture first is carried out with Gause I synthetic media, obtains solid seed
Culture medium carries out sporozoites inoculation on fermentation material, frankia microbial inoculum raw material is obtained after fermented and cultured, by handling
To frankia bacterium powder.
8. a kind of Citrus Huanglongbing pathogen special biological agent, which is characterized in that be used for mandarin orange including claim 1-7 any one of them
The obligate AM Inoculants of complex micro organism fungicide, microbial adsorbent, citrus of tangerine yellow twig, and moisture is 15-20%.
9. the preparation method of Citrus Huanglongbing pathogen special biological agent according to any one of claims 8, which is characterized in that including walking as follows
Suddenly：
(1) microbial adsorbent is added into the microbial bacterial agent prepared；
(2) it is mixed again with the obligate AM Inoculants of citrus；
(3) adjust mixed preparation moisture to 15-20% to get.
10. the application method of Citrus Huanglongbing pathogen special biological agent according to any one of claims 8, includes the following steps：
A, by biological agent according to 1:The weight ratio of 500-800 is diluted with water, and stands 5-20 minutes；
B, the clear liquid on the mixed solution top after standing is sprayed for blade face and branch, the suspension of lower part and insoluble substrate,
For irrigating tree root.
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| CN116179446A (en) * | 2023-03-22 | 2023-05-30 | 天津坤禾生物科技集团股份有限公司 | Microbial agent for preventing and treating citrus root rot yellowing disease, and preparation method and application thereof |
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Application publication date: 20181009 |