CN114686387A - Ensiformula mucilaginosa and application thereof in preparation of microbial fertilizer - Google Patents
Ensiformula mucilaginosa and application thereof in preparation of microbial fertilizer Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims description 11
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- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 21
- 230000002708 enhancing effect Effects 0.000 claims abstract description 11
- 241001528536 Ensifer adhaerens Species 0.000 claims abstract description 5
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention discloses a sword fungus adhesion, which is classified and named as sword fungus adhesion (A)Ensiferadhaerens) The strain number is CN-02, and the strain number is CGMCC 21357, which is preserved in the China general microbiological culture Collection center; further discloses the application of the Zygomyces invicta in enhancing the salt stress resistance of crops. The invention discloses a microbial inoculum which takes sword-shaped bacteria as active microorganisms; further discloses application of the microbial inoculum in enhancing salt stress resistance of crops. The Zygomyces adhesins can be adhered to the root system of the plant and can also enter the plant to be symbiotic with the plant, so that the absorption of the plant to external nutrient elements is improved, the metabolic system of the plant is improved, the water potential in the plant is maintained, and the like, thereby promoting the growth and development of the plant, improving the yield, and simultaneously enhancing the salt stress resistance of the plant. To give full play to the ecological fertility of soil and maintainThe balance of the agricultural ecological environment and the like have extremely important significance and application value.
Description
Technical Field
The invention relates to the field of microbial agents, and particularly relates to sword-shaped bacterium adhesion and a microbial agent and application thereof.
Background
Soil salinization and soil secondary salinization caused by irrigation are main environmental risks caused by natural or human activities and generally occur in regions with dry climate, high soil evaporation strength, high underground water level and more soluble salts. Statistically, 20% of cultivated land and nearly half of irrigated land in the world are threatened by salt damage to different degrees, and the proportion is rising. The total area of the salinized land in China is about 1 hundred million hectares, and the salinized land is mainly distributed in 17 provinces including northwest, northeast, north China and coastal areas. When the soil salt concentration reaches a certain value, the normal life activities of plant cells can be damaged, the growth of plants is inhibited, the land output rate is low, the comprehensive agricultural productivity is seriously insufficient, and the ecological safety and the economic sustainable development of the regions are directly influenced.
At present, how to improve saline-alkali resistance and saline-alkali resistance of crops becomes a major challenge for researchers. The key to the problem of promoting the growth of plants in the halophytic environment is to improve the soil and improve the saline-alkali resistance of crops. Plant growth-promoting rhizobacteria (PGPR) is a type of microorganism in Plant rhizosphere soil which can promote Plant growth, prevent and control diseases and increase crop yield. The use of PGPR will help to reduce the adverse effects of salinity on plants.
Disclosure of Invention
The purpose of the invention is as follows: in order to solve the problem of salt resistance of plants, the invention provides a sword-shaped bacterium adhesion, and further provides a microbial inoculum taking the sword-shaped bacterium adhesion as an active microorganism and application of the microbial inoculum in enhancing the salt stress resistance of crops.
The technical scheme is as follows: the sword-like fungus adhesion is classified and named as sword-like fungus adhesion (Ensiferadhaerens), the strain number is CN-02, the sword-like fungus adhesion has been preserved in the China general microbiological culture Collection center, the preservation time is 12 months and 11 days in 2020, the preservation number is CGMCC NO.21357, the preservation address is No. 3 of Xilu No.1 of Xingyang district in Beijing, the preservation center of the institute of microbiology in China, and the postal code is 100101. The strain is a strain bred by the inventor from the soil of the paddy field of the Putouu region of Nanjing in 2020 and 5 months.
Ensifer adhesins CN-02 has the following properties:
1. morphological characteristics of colonies
After culturing for 16-24h at 30 ℃ on LB solid culture medium, the cells are observed to be single cells and rod-shaped by a microscope, the length of the bacteria is 1.0-1.5 μm, and the width is 0.4-0.6 μm. Belongs to gram-negative bacteria and no spores. The thalli can grow in a large amount after being cultured in the culture medium for 12 hours at the temperature of 30 ℃, and bacterial colonies are milky, opaque, smooth, sticky, convex and regular in edge.
2. 16SrDNA sequence analysis
The length of the 16SrDNA sequence is 1388bp, and the nucleotide sequence is shown in a sequence table SEQ. NO. 1. And (3) comparing the 16SrDNA sequence with related species in a GeneBank database to construct a phylogenetic tree based on the 16SrDNA complete sequence. The results show that: the strain CN-02 and the Zygomyces sticklandicus reach 100 percent of homology. Therefore, the bacterial strain of the invention is determined to be Zygomyces sticklandicus, in particular to Ensiferahrens CN-02.
The invention further provides application of the sword-like fungus adhesion in any one or more of improving crop yield, improving crop quality and enhancing crop salt resistance. The sword-shaped fungus CN-02 can produce extracellular polysaccharide which has water absorption and can maintain osmotic pressure inside plants under the condition of high permeability through symbiosis with the plants, thereby having the capability of enhancing the salt resistance of the plants. The extracellular polysaccharide producing strain can be mixed with an organic fertilizer for use, and can also be used independently, so that the rhizosphere microbial environment is improved, the plant growth is promoted, the salt resistance of the plant is enhanced, and the using amount of a chemical fertilizer is reduced.
Preferably, the crops are grain crops, vegetable crops or melon and fruit crops.
The invention further provides an application of the sword-like bacteria adhesion in preparation of exopolysaccharide through fermentation, which comprises the following preparation steps: inoculating the sword-like bacterium to an LB culture medium, activating for 12-24h at 25-35 ℃, transferring the activated sword-like bacterium to a fermentation culture medium, and culturing for 36-50h at 30-35 ℃ to obtain a fermentation liquid, wherein the extracellular polysaccharide yield in the fermentation liquid is up to 7.4 g/L.
The formula of the fermentation medium is as follows: 10-100g/L of carbon source, 1-30g/L of nitrogen source, 0.01-50g/L of inorganic salt, pH5.0-9.0, and the solvent is water.
The carbon source is any one or more of glucose, lactose, xylose, citric acid and starchA combination of species; the nitrogen source is peptone, yeast extract, corn steep liquor, (NH)4)2SO4、NH4Cl、(NH4)2HPO4And NH4NO3Any one or a combination of several of them; the inorganic salt is any one or combination of more of sulfate, phosphate, dihydrogen phosphate, dibasic hydrogen phosphate and hydrochloride.
The invention further provides an active microbial agent which comprises the sword-shaped bacteria adhesion.
The invention further provides a preparation method of the microbial inoculum, which comprises the following steps: inoculating the Zygophyllum adhesicum to an LB culture medium, activating for 12-24h at 25-35 ℃, transferring the activated Zygophyllum adhesicum to a fermentation culture medium, culturing for 36-50h at 30-35 ℃ to obtain fermentation liquor, and performing aftertreatment on the fermentation liquor or the fermentation liquor to obtain a system which is a microbial inoculum, namely the fermentation liquor, concentrated solution obtained by concentrating the fermentation liquor or powder obtained by drying the fermentation liquor is the microbial inoculum.
The formulation of the microbial inoculum is solution, suspension, granules, powder or stick agent, and the concentration of the microbial inoculum in the microbial inoculum is 1 multiplied by 108-9×109CFU/g。
The LB culture medium has the following formula: 10g/L of peptone, 5g/L of yeast powder, 10g/L of sodium chloride, pH6.0-8.0, and the solvent is water.
The formula of the fermentation medium is as follows: 10-100g/L of carbon source, 1-30g/L of nitrogen source, 0.01-50g/L of inorganic salt, pH5.0-9.0, and the solvent is water.
The carbon source is any one or combination of more of glucose, lactose, xylose, citric acid and starch; the nitrogen source is peptone, yeast extract, corn steep liquor, (NH)4)2SO4、NH4Cl、(NH4)2HPO4And NH4NO3Any one or a combination of several of them; the inorganic salt is any one or combination of more of sulfate, phosphate, dihydrogen phosphate and hydrochloride.
The invention further provides application of the microbial inoculum in any one or more of improving crop yield, improving crop quality and enhancing crop salt resistance; the microbial inoculum is applied to crops and garden crops through root irrigation or spraying treatment. The microbial inoculum can be mixed with organic fertilizer for use or used independently, improves rhizosphere microbial environment, promotes plant growth, enhances plant salt resistance, and reduces fertilizer usage amount.
Preferably, the crops are grain crops, vegetable crops or melon and fruit crops.
Has the advantages that: (1) the method comprises the steps of screening out the sword-shaped bacterium adhesion CN-02 which has the functions of enhancing the salt resistance of plants and producing extracellular polysaccharide from soil, and fermenting and culturing by taking the sword-shaped bacterium adhesion CN-02 as a strain to obtain the sword-shaped bacterium adhesion CN-02 microbial inoculum. The sword-like fungus CN-02 can be adhered to the root system of the plant, and can also enter the plant to be symbiotic with the plant, thereby improving the absorption of the plant to external nutrient elements, improving the metabolic system of the plant, maintaining the water potential in the plant and the like, promoting the growth and development of the plant, improving the yield, and enhancing the drought resistance of the plant. Has extremely important significance and application value for fully exerting the ecological fertility of soil, keeping the balance of agricultural ecological environment and the like. (2) The sword fungus adhesion CN-02 microbial inoculum does not discharge pollutants to the environment in the production process, and the sword fungus adhesion CN-02 is an environment-friendly fertilizer in the real sense; the preparation method of the sword-like fungus adhesion CN-02 microbial inoculum provided by the invention has the advantages of simple production process, high production efficiency, capability of realizing industrial mass production and good economic effect and social effect.
Detailed Description
Example 1 screening and identification of Ensifer Citrini
The sword-shaped fungus adhesion CN-02 is a strain bred from the soil of a soup spring town paddy field in Pukou of Nanjing by the inventor (taking polysaccharide production as a standard), and the screening process is as follows:
and dissolving the collected soil sample in sterile water, shaking and uniformly mixing. The sample solution is diluted in gradient, 10 is taken-4、10-5、10-6The dilutions of the three concentration gradients were spread evenly on solid medium (medium composition: sucrose 20g/L, peptone 5g/L, beef extract 1g/L, NaCl 5g/L, agar 20 g/L). Culturing in 30 deg.C constant temperature incubator for 48h, viewing the plate every day during the period,the colony characteristics were observed, and the sticky colonies were used as the screening target. Selecting a single colony which can produce high-viscosity extracellular polysaccharide from the culture medium, and purifying the single colony on a solid culture difficult plate by a scribing method.
And respectively inoculating the single colonies obtained in the previous step into a liquid culture medium (the components of the culture medium are 20g/L of cane sugar, 5g/L of peptone, 1g/L of beef extract and 5g/L of NaCl), performing shake fermentation culture at 30 ℃ on a shaking table, and comparing the viscosity of fermentation liquids of different strains after 48 hours, wherein the strain with the highest viscosity of the fermentation liquid is the Ensifer adhesivelis CN-02.
Ensifer adhesins CN-02 has the following properties:
after culturing for 16-24h at 30 ℃ on LB solid culture medium, the cells are observed to be single cells and rod-shaped by a microscope, the length of the bacteria is 1.0-1.5 μm, and the width is 0.4-0.6 μm. Belongs to gram-negative bacteria, flagellum and spore. The thalli can grow in a large amount after being cultured in the culture medium for 12 hours at the temperature of 30 ℃, and bacterial colonies are milky, opaque, smooth, sticky, convex and regular in edge. The 16S rDNA of the adhaerens CN-02 is shown in a sequence table SEQ ID No.1, and the sequence total length is 1388 bp. The strain of the invention is identified as Zygosaccharomyces adherens and named CN-02 by comparison of blastN sequence alignment tools in NCBI database (https:// www.ncbi.nlm.nih.gov /).
Example 2 Sword bacterium adhesion CN-02 microbial inoculum
Activating E.adhaerens CN-02 stored at a 4 ℃ inclined plane, inoculating the activated E.adhaerens CN-02 to an eggplant bottle culture medium (namely an activation culture medium), wherein the activation temperature is 30 ℃ and the activation time is 16 h. Picking a large amount of strains on eggplant bottles, inoculating the strains in a fermentation medium, culturing at 30 ℃ for 48h with the ventilation of sterile air in a fermentation tank of 0.2vvm and the stirring speed of 200rpm to obtain fermentation liquor, wherein the number of the strains in the strain can reach 5 multiplied by 109CFU/g is higher than the standard.
The activation medium is an LB medium, and the fermentation medium comprises the following components: 40g/L of glucose, 8g/L of beef extract, 6g/L of NaCl, 10g/L of peptone and 7.0 of pH.
Example 3 determination of the ability of Excercospora invigora CN-02 to produce exopolysaccharides
The content of exopolysaccharide in the fermentation broth of example 2 was quantitatively determined by phenol-sulfuric acid method, and the exopolysaccharide production amount of Exoecium griffithii CN-02 was calculated from a standard curve prepared with anhydrous glucose. The result shows that the yield of the Exoecaria californica CN-02 exopolysaccharide reaches 7.4 g/L.
Example 4 Sword bacterium adhesion CN-02 can obviously improve the yield of Chinese cabbage
The preparation method of the microbial inoculum used by the invention is the same as that of the example 2.
And (3) taking the Chinese cabbage as an experimental object, and developing a pot experiment.
The experiment is provided with 4 treatment modes:
treatment 1 (T)1) Watering normally; treatment 2 (T)2) Adding 0.6% of salt when watering for the first time; treatment 3 (T)3) Normal watering and application are carried out; the microbial inoculum prepared in example 2 (5 mL of microbial inoculum is added in each pot, and the water content of the microbial inoculum is counted in the watering amount); treatment 4 (T)4) When watering for the first time, 0.6% of salt is added, and the microbial inoculum prepared in example 2 is applied (5 mL of microbial inoculum is added in each pot, and the water content of the microbial inoculum is counted in the watering amount).
Treatments 3 and 4 the inoculum was applied by a spray method.
The application amounts and daily management of the four treated other conventional fertilizers are consistent, the yield and other indexes are detected in the harvest period of the Chinese cabbage, and the experimental results are shown in table 1.
TABLE 1 growth conditions under different treatment conditions in Chinese cabbage potting experiments
| Treatment of | Root length (cm) | Leaf length (cm) | Fresh weight (g) | Increase the yield |
| T1 | 6.9±0.4 | 29.3±0.3 | 142.3±1.6 | — |
| T2 | 6.2±0.6 | 18.9±0.5 | 96.7±4.3 | -32.04 |
| T3 | 13.2±0.5 | 32.3±0.6 | 170.5±1.4 | 19.8 |
| T4 | 12.6±0.7 | 28.6±0.4 | 149.7±2.5 | 5.2 |
In a Chinese cabbage pot experiment, compared with the treatment 1 in the treatment 3 under normal watering amount, the root length, the leaf length and the fresh weight are obviously improved, and the yield is increased by 19.8 percent; the yield of the treatment 2 is reduced by 32.04 percent; when the microbial inoculum is applied, the yield of the treatment 4 is increased by 5.2%, and compared with the treatment 2, the root length, the leaf length and the fresh weight are greatly improved. The Exoecaria rhizincicola CN-02 can not only promote the growth of crops, but also has very obvious excellent performance on enhancing the salt resistance of the crops.
Example 5 Sword bacterium adhesion CN-02 can significantly improve wheat yield
The preparation method of the microbial inoculum used in the invention is the same as that of the embodiment 2.
And (3) taking wheat as a research object to carry out a cell experiment.
The experiment sets 3 processing modes, each processing mode is set with 3 times of repetition, 9 cells are counted, and the area of each cell is 5m multiplied by 4m which is 20m2And the cells are randomly distributed.
Treatment 1(T1) was a blank without addition of the microbial inoculum; treatment 2(T2) the inoculum prepared in example 2 (16 kg/hm)2) (ii) a Treatment 3(T3) application of inactivating agent (16 kg/hm)2The inoculum prepared in example 2 was inactivated at high pressure and high temperature).
Treatments 2 and 3 were applied in a root irrigation manner.
The other conventional fertilizer application rates and daily management of the three treatments were consistent. The experimental results of various indexes such as wheat yield and the like detected in the mature period of wheat are shown in table 2.
TABLE 2 yield profile of wheat under different treatment conditions
The result shows that in the wheat experiment, the swordlike bacillus adhesion CN-02 obviously promotes the growth of wheat; the yield is improved by 37.1 percent, the thousand kernel weight is also obviously improved, and the yield is almost the same as that of a control group after the sword-like fungus CN-02 is inactivated. This shows that the quantity and quality of crop can be improved significantly by the aid of the sword-like fungus CN-02.
Example 6 Sword bacterium adhesion can significantly improve the yield and quality of watermelon
And (3) carrying out a cell experiment by taking the watermelon as a research object.
The experiment sets 3 processing modes, each processing mode is set with 3 times of repetition, 9 cells are counted, and the area of each cell is 5m multiplied by 4m which is 20m2And each cell is randomly distributed.
T1Treating without adding bacteria agent, namely blank group; t is2Seeds are placed in a container, and then the microbial fertilizer (200 ml/acre) of the invention is diluted 50 times for seed dressing.
The other conventional fertilizer application rates and daily management for both treatments were consistent. The indexes such as watermelon yield and the like are detected in the mature period of the watermelon, and the experimental result is shown in table 3.
TABLE 3 yield and quality of watermelon under different treatment conditions
The results show that, in the watermelon experiment, compared with the control group, the sword-like fungus CN-02 obviously improves the content of vitamin C in the watermelon, and simultaneously improves the sugar degree, fruit setting rate and yield of the watermelon. This shows that the quantity and quality of watermelon are improved significantly by the aid of the sword-like fungus CN-02.
Sequence listing
<110> Suzhou Kening polyol Co., Ltd
<120> sword-like bacterium adhesion strain and application thereof in preparation of microbial fertilizer
<141> 2020-12-28
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1388
<212> DNA
<213> 16SrDNA(2 Ambystoma laterale x Ambystoma jeffersonianum)
<400> 1
ctcgctgctt actaccgtgg ttagctgcct ccttgcggtt agcgcactac cttcgggtag 60
aaccaactcc catggtgtga cgggcggtgt gtacaaggcc cgggaacgta ttcaccgcag 120
catgctgatc tgcgattact agcgattcca acttcatgca ctcgagttgc agagtgcaat 180
ccgaactgag atggcttttg gagattagct cgacctcgcg gtctcgctgc ccactgtcac 240
caccattgta gcacgtgtgt agcccagccc gtaagggcca tgaggacttg acgtcatccc 300
caccttcctc tcggcttatc accggcagtc cccttagagt gcccaaccaa atgctggcaa 360
ctaagggcga gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga 420
cgacagccat gcagcacctg tctccgatcc agccgaactg aaggaaaacg tctccgtaat 480
ccgcgatcgg gatgtcaagg gctggtaagg ttctgcgcgt tgcttcgaat taaaccacat 540
gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt tttaatcttg cgaccgtact 600
ccccaggcgg aatgtttaat gcgttagctg cgccaccgaa cagtaaactg cccgacggct 660
aacattcatc gtttacggcg tggactacca gggtatctaa tcctgtttgc tccccacgct 720
ttcgcacctc agcgtcagta atggaccagt gagccgcctt cgccactggt gttcctccga 780
atatctacga atttcacctc tacactcgga attccactca cctcttccat actctagaca 840
cccagtatca aaggcagttc cggggttgag ccccgggatt tcacccctga cttaaatgtc 900
cgcctacgtg cgctttacgc ccagtaattc cgaacaacgc tagccccctt cgtattaccg 960
cggctgctgg cacgaagtta gccggggctt cttctccggt taccgtcatt atcttcaccg 1020
gtgaaagagc tttacaaccc tagggccttc atcactcacg cggcatggct ggatcaggct 1080
tgcgcccatt gtccaatatt ccccactgct gcctcccgta ggagtttggg ccgtgtctca 1140
gtcccaatgt ggctgatcat cctctcagac cagctatgga tcgtcgcctt ggtaggcctt 1200
taccccacca actagctaat ccaacgcggg ctcatccttt cccgataaat ctttccccca 1260
aagggcttat acggtattag cacaagtttc cctgcgttat tccgtagaaa agggtagatt 1320
cccacgcgtt actcacccgt ctgccgctcc ccttgcgggg cgctcgactg catgggaagc 1380
tgccgcaa 1388
Claims (9)
1. A strain of sword-like fungus with adhesion CN-02, which is named as sword-like fungus with adhesion (A)Ensifer adhaerens) The biological preservative has been preserved in China general microbiological culture Collection center (CGMCC), the preservation time is 12 months and 11 days in 2020, and the preservation number is CGMCC number 21357.
2. Use of the sword-like fungus CN-02 adhesion of claim 1 in the preparation of microbial fertilizers.
3. The use of the sword-like fungus CN-02 of claim 1 for any one or more of increasing crop yield, improving crop quality, and enhancing abiotic stress resistance of crops.
4. The use of the Ensifer viscosus CN-02 of claim 1 for the production of exopolysaccharides with high viscosity.
5. An active microbial agent, comprising the sword-like fungus CN-02 according to claim 1.
6. The preparation method of the active microbial agent as claimed in claim 5, characterized in that the sword-shaped bacteria CN-02 is inoculated in LB culture medium and activated at 25-35 ℃ for 12-24h, the activated sword-shaped bacteria is transferred in fermentation medium and cultured at 30-35 ℃ for 36-50h to obtain fermentation liquor, and the fermentation liquor or the system obtained by post-treatment of the fermentation liquor is the microbial agent.
7. The method according to claim 6, wherein the microbial inoculum is prepared in the form of a solution, a suspension, granules or a powder, and the concentration of the microbial inoculum in the microbial inoculum is 1X 108-9×109 CFU/g。
8. The use according to claim 3, wherein the application to plants is carried out by root watering, spraying and seed soaking.
9. The use according to claim 8, wherein the crops comprise crops, garden crops.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115404183A (en) * | 2022-08-24 | 2022-11-29 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application |
| CN116024123A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of sword bacteria S2_8_1 in promoting soil nitrification |
| CN116024124A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of Sword bacteria S2_8_1 in promoting plant to synthesize cytokinin |
| CN116024123B (en) * | 2022-09-26 | 2024-06-04 | 河南科技大学 | Application of sword bacteria S2_8_1 in promoting soil nitrification |
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| CN102851237A (en) * | 2012-08-07 | 2013-01-02 | 南京师范大学 | Ensiferadhaerens and application thereof |
| EP3058825A1 (en) * | 2015-02-19 | 2016-08-24 | Futureco Bioscience, S.A. | Bacteria with nematicidal activity |
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| CN102851237A (en) * | 2012-08-07 | 2013-01-02 | 南京师范大学 | Ensiferadhaerens and application thereof |
| EP3058825A1 (en) * | 2015-02-19 | 2016-08-24 | Futureco Bioscience, S.A. | Bacteria with nematicidal activity |
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| K. H. HUANG ET AL.: "Optimization of exopolysaccharide production and diesel oil emulsifying properties in root nodulating bacteria", 《WORLD J MICROBIOL BIOTECHNOL》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115404183A (en) * | 2022-08-24 | 2022-11-29 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application |
| CN115404183B (en) * | 2022-08-24 | 2024-01-26 | 河南科技大学 | Ammonia oxidizing bacteria S2_8_1 with mixed nutrition characteristics and application thereof |
| CN116024123A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of sword bacteria S2_8_1 in promoting soil nitrification |
| CN116024124A (en) * | 2022-09-26 | 2023-04-28 | 河南科技大学 | Application of Sword bacteria S2_8_1 in promoting plant to synthesize cytokinin |
| CN116024123B (en) * | 2022-09-26 | 2024-06-04 | 河南科技大学 | Application of sword bacteria S2_8_1 in promoting soil nitrification |
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