CN110713953B - Mesorhizobium strain with phosphate solubilizing property and application thereof - Google Patents
Mesorhizobium strain with phosphate solubilizing property and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
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Abstract
The invention discloses a rhizobium intermedium strain with phosphate solubilizing capability and application thereof, wherein the strain is a rhizobium intermedium (mesorhizobium.sp) strain YTR-M3 which is preserved in China general microbiological culture collection center in 2019, 4 and 9 months, and the preservation number is CGMCC No. 17532. The invention screens out a high-efficiency phosphate solubilizing bacterium from the soil of a cell for a long-term positioning test of organic fertilization of a hawkthorn-pond red soil ecological experimental station of Chinese academy of sciences, identifies the phosphate solubilizing bacterium, proves that the strain YTR-M3 has high-efficiency phosphate solubilizing characteristic through experiments, is mixed with wood vinegar for use, obviously increases the content of available phosphorus in red soil, and improves the growth condition of corn.
Description
Technical Field
The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an intermediate rhizobium strain YTM-3 and application thereof in phosphate solubilizing.
Background
Phosphorus is the second most inorganic nutrient element necessary for plants,participate in a plurality of important enzymatic reactions and metabolic regulation in plants. Therefore, the phosphorus has important significance on the growth and metabolism of plants. The red soil in the southeast hilly area is mainly distributed in subtropical and tropical regions, including Hunan, Jiang, Zhe, Min, Gui, Yue, Qiong, Huo, and Wannan, and the area is about 113 km2And accounts for 11.8 percent of the total land area in China. The phosphorus in the red soil exists in various chemical forms, wherein the inorganic phosphorus accounts for 35-70% of the total phosphorus, and the organic phosphorus accounts for 30-65% of the total phosphorus. However, phosphorus in red soil is easily affected by metal ions Al3+、Fe3+And Ca2+The solubility and the mobility of phosphorus are reduced due to the adsorption and the combination and the fixation, so that the red soil is in a state of serious shortage of available phosphorus of phosphorus, and the utilization rate of phosphate fertilizer is reduced. The application of a large amount of phosphate fertilizer has become one of important measures for relieving the stress of soil red soil phosphorus and realizing high and stable yield of crops for a long time. According to the existing phosphate fertilizer production capacity, the non-renewable phosphate ore resources are exhausted within 60-90 years. Therefore, the utilization rate of the phosphorus element and the phosphate fertilizer in the red soil is improved, and the exploitation of the potential of the phosphate fertilizer resource has important significance for the sustainable development of agriculture in China.
The phosphorus-dissolving bacteria can convert insoluble or insoluble phosphorus in red soil into available phosphorus which is easily absorbed and utilized by plants through metabolites such as organic acid, phosphatase and the like secreted by the phosphorus-dissolving bacteria, so that the phosphorus supply level of soil is improved, the phosphorus absorption amount of crops is increased, and the yield of the crops is improved; meanwhile, the absorption of crops to other nutrient elements such as zinc, copper and the like in the root system of the plant can be promoted, the disease resistance of the plant is enhanced, and the environmental pollution is reduced, so that the microbial inoculum prepared by the phosphate solubilizing bacteria has great development and application values. The screening of the phosphate solubilizing bacteria is mainly concentrated in the saline-alkali soil area, however, the functions of the phosphate solubilizing bacteria are seriously limited due to the acidic and barren characteristics of the red soil, and even if the strains with good effects are locally applied, the effects are obviously reduced when the strains are added into the acidic red soil. Separating and screening high-efficiency indigenous phosphate-solubilizing bacteria suitable for being applied to dry land red soil, optimizing the culture conditions of the high-efficiency indigenous phosphate-solubilizing bacteria, preparing a microbial inoculum and an organic fertilizer to be applied to acidic dry land red soil with low effective phosphorus content, so as to exert the high-efficiency phosphate-solubilizing effect of the acid dry land red soil, improve the utilization efficiency of the phosphate fertilizer of the red soil farmland, partially replace the inorganic phosphate fertilizer and promote the yield increase of corn.
At present, phosphate-solubilizing bacteria bacterial fertilizers suitable for certain soil and crops are developed in China and applied to the production of crops such as melons, fruits, vegetables, peanuts, traditional Chinese medicinal materials, wheat and the like in some areas, but research and development reports of phosphate-solubilizing bacteria suitable for acidic red soil and promoting corn yield increase are rare.
Disclosure of Invention
The first purpose of the invention is to provide an intermediate rhizobium strain with phosphate-solubilizing characteristics, so as to improve the activation and supply capacity of the red soil phosphorus and promote the growth and yield of corn plants.
In order to achieve the purpose, the invention adopts the technical scheme that:
an intermediate rhizobium strain with phosphate solubilizing capability, which is an intermediate rhizobium (mesorhizobium.sp) strain YTR-M3, which is preserved in the China general microbiological culture Collection center at 4 and 9 months in 2019, and has the address: the collection number of the microbial research institute of Chinese academy of sciences, No. 3 of Xilu No.1 of Beijing, Chaoyang, and the institute of microbiology is CGMCC No. 17532.
The intermediate rhizobium strain YTR-M3 is separated from corn rhizosphere soil in a cell of an organic fertilization long-term positioning test of a Yingtan red soil ecological experimental station of Chinese academy of sciences, and is diluted by soil suspension, inoculated, cultured, separated and purified by an LB culture medium to obtain an intermediate rhizobium (Mesorhizobium amorphae) strain which is named as YTR-M3 and has the gene registration number of MK138663 at Genbank.
The biological properties of the strain are respectively as follows:
(1) morphological characteristics: obvious round and milky colony is formed on an LB culture medium, the edge is neat, the colony is convex and non-transparent, the surface is smooth and wet, gram staining is negative, and the shape of the cell is rod-shaped under the observation of an electron microscope.
(2) The culture characteristics are as follows: the growth temperature range is 22-28 ℃, the optimum pH is 7.0, and the culture medium is aerobically or facultatively anaerobic and grows in LB culture medium. The components of the culture medium: 10.0g/L of Tryptone, 5.0g/L of Yeast extract and 10.0g/L of NaCl.
It is a second object of the present invention to provide a microbial agent comprising the Mesorhizobium sp strain YTR-M3.
The third purpose of the invention is to provide the application of the intermediate rhizobium (Mesorhizobium. sp) strain YTR-M3, which has efficient phosphate solubilizing property, can decompose inorganic phosphorus and organic phosphorus, such as aluminum phosphate, iron phosphate and lecithin, and can remarkably increase the effective phosphorus content in red soil.
Preferably, the intermediate rhizobium strain YTR-M3 can be used for improving the growth condition of corn, and the bacterial liquid of the intermediate rhizobium strain YTR-M3 is inoculated into red soil to improve the growth condition of the corn, so that the biomass of the overground part and the underground part of the corn can be increased, the content of soluble sugar and soluble protein of the corn leaves can be increased, and the content of available phosphorus in the red soil can be increased.
Preferably, the intermediate rhizobium strain YTR-M3 is co-administered with wood vinegar to improve the growth of corn.
Has the advantages that: the invention screens out a high-efficiency phosphate solubilizing bacterium from the soil of a cell for a long-term positioning test of organic fertilization of a hawkthorn-pond red soil ecological experimental station of Chinese academy of sciences, identifies the phosphate solubilizing bacterium, and proves that YTR-M3 has high-efficiency phosphate solubilizing characteristic through experiments, is mixed with wood vinegar for use, obviously increases the content of available phosphorus in red soil, and improves the growth condition of corn.
Drawings
FIG. 1 is a characteristic diagram of the colony of the isolated intermediate rhizobium strain YTR-M3 on a solid medium and an electron micrograph;
FIG. 2 is a picture of the phosphate solubilizing circle of the strain YTR-M3;
FIG. 3 is a diagram of the construction of phylogenetic tree of the strain YTR-M3;
FIGS. 4 a-4 d show the soluble sugar, soluble protein, pot soil available phosphorus content and the biomass of the upper and lower parts of the corn field, respectively, for potted corn inoculated with Rhizobium intermedium YTR-M3.
Detailed Description
The present invention will be further explained with reference to examples.
The present invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the specific material ratios, process conditions and results thereof described in the examples are illustrative only and should not be taken as limiting the invention as detailed in the claims.
Example 1
Screening and identification of Mesorhizobium sp strain YTR-M3
1. Screening process
(1) Weighing 1g of high-fertilizer treated rhizosphere soil taken from a long-term positioning test land of a Jiangxi Yingtan red soil test station, putting the high-fertilizer treated rhizosphere soil into a centrifuge tube, and adding 9mL of 0.9% physiological saline to prepare 10-1The bacterial suspension is put into a shaking box of 180r/min to shake for 10min, and then is taken out and stands for 1 h.
(2) Sucking 5 mul of settled soil suspension under aseptic condition, adding 45 mul of sterile water and diluting to prepare 10-2And (4) bacterial suspension. Sequentially diluting the bacterial suspension to 10-3、10-4、10-5Respectively taking 5mL 10-5The bacterial suspension is added into LB culture medium of 50mL and 500mL to prepare 10-6And 10-7The culture solution of (1), mixing-6And 10-7The culture broth of (2) was added to a 96-well plate, 250. mu.L of the culture broth was added to each well, and three plates were added to each concentration. And finally, putting the 96-well plate into an enzyme-labeling instrument, and observing the growth condition of the 96-well plate under room-temperature culture.
(3) And (3) taking 200 mu L of liquid in the pore diameter of the bacteria liquid growing in the plate, putting the liquid into a preservation tube, adding 200 mu L of glycerin for preservation, marking the corresponding pore plate and pore serial number, and using the remaining 50 mu L for colony PCR amplification. PCR amplification conditions: pre-denaturation at 94 ℃ for 10min, denaturation at 94 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 1min, 35 cycles of denaturation and annealing extension, extension at 72 ℃ for 10min, and reaction termination. The effective sequences obtained after sequencing are compared on NCBI, and the result shows that the homology with rhizobium intermedium is highest.
The gene sequence of Mesorhizobium sp strain YTR-M3 is shown in SEQ ID NO. 1.
Example 2
Phosphate solubilizing Properties of Mesorhizobium (Mesorhizobium. sp.) Strain YTR-M3
(1) The strain YTR-M3 is preserved in glycerin tube, and is streaked on LB solid culture medium after being activated for later use.
(2) Preparing culture medium plates of Mengdina organophosphorus (lecithin), aluminum phosphate and iron phosphate, dividing each plate into 6 areas, sequentially sucking 6 mu L of points at the center of each area, culturing at 25 ℃ for 4 days at a rotating speed of 180r/min, observing and recording the diameter of a phosphorus-dissolving ring (unit D/cm) and the diameter of a bacterial colony (unit D/cm), calculating the D/D ratio to determine the phosphorus-dissolving capacity and the phosphorus-dissolving efficiency of the strain to different insoluble phosphorus, wherein the formula of the culture medium is ① Mengdina organophosphorus (lecithin) bacterial culture medium (1L) glucose 10.0g, (NH)4)2SO40.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO40.03g，MnSO4·H2O 0.03g，KCl0.3g，CaCO31.0g, lecithin 0.3g, agar 20g, pH 7.0. ② Monkinna inorganic phosphorus (aluminum phosphate) bacteria culture medium (1L) glucose 10.0g, (NH)4)2SO40.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO40.03g，MnSO4·H2O0.03g，KCl 0.3g，CaCO31.0g，AlPO43.93g, agar 20g, pH 7.0.3○ Monkinna inorganic phosphorus (iron phosphate) bacteria culture medium (1L) glucose 10.0g, (NH)4)2SO40.5g，NaCl 0.3g，MgSO4·7H2O 0.3g，FeSO40.03g，MnSO4·H2O 0.03g，KCl 0.3g，CaCO31.0g，FePO44.86g, agar 20g, pH 7.0. The results of the phosphate solubilizing cycle are shown in FIG. 3. The dissolving capacity of the strain to soluble phosphorus is shown in table 1;
TABLE 1 Rhizobium intermedium strain YTR-M3 phosphorus solubilization characterization
The intermediate rhizobium (mesorhizobium. sp) strain YTR-M3 has good growth vigor on three indissolvable phosphorus culture media for testing, the strain YTR-M3 can not only decompose inorganic phosphorus but also organic phosphorus, and the diameters of colonies on a solid culture medium are as follows in sequence: iron phosphate > aluminum phosphate > lecithin. From this, it was found that the solubility of the strain to Fe-P was strong. And Fe-P exists in the red soil in a large amount, so the strain has great application value in solving the problem of phosphorus deficiency of the red soil.
Example 3
Experiment of potted corn
1. The experiment was performed with 3 treatments, control, wood vinegar (wood vinegar containing amino acids combined with water soluble fertilizer), wood vinegar + microbial inoculum (YTR-M3), and 4 replicates each.
2. The using amount of soil and fertilizer: the amount of soil in each 10X 10(cm) pot was about 500g, and the treatment of applying pyroligneous liquor added 15mL of pyroligneous liquor (1:800 dilution) per pot as a base fertilizer. And then applied once every other week by means of root drip irrigation and foliage spray.
3. Seedling culture: 1) sterilizing seeds, selecting proper amount of seeds, sterilizing with 70% alcohol for 1min, sterilizing with 1% sodium hypochlorite for 15min, and washing with sterile water for five times. Placing the sterilized seeds on sterile water, and placing the seeds in an illumination incubator at 28 ℃ until the seeds germinate; 2) and selecting the seedlings with consistent growth vigor on the fifth day of seed germination and transplanting the seedlings into the red soil.
4. Adding a bacterial liquid: applying bacterial liquid in the second week after transplanting the corn seedlings, respectively culturing 100mL YTR-M3 bacterial liquid in LB culture medium in a shaking way, respectively measuring OD of three bottles of fermentation liquid after culturing for 18h600Value, when OD600When the value reaches 1, the concentration of the bacterial liquid is about 1 multiplied by 109And CFU, centrifuging the bacterial liquid according to the amount of 100 mu L per gram of soil, washing and precipitating for 3 times by using 0.9% physiological saline, finally suspending the thalli by using sterile water to prepare a microbial inoculum, and adding the microbial inoculum into the soil.
5. The corn is harvested after growing in the soil for about one month, and the underground biomass of the overground part of each treated corn seedling, soluble sugar and soluble protein in leaves and the soil available phosphorus content in the corn harvesting period are respectively measured. The results are shown in FIG. 4.
The experiment shows that compared with the control group, the treatment of adding the pyroligneous liquor and the intermediate rhizobium (mesorhizobium sp) strain YTR-M3 obviously increases the content of soluble sugar and soluble protein in the corn leaves and the biomass of the underground parts of the overground part, and obviously improves the growth condition of the corn. The content of the available phosphorus in the pot culture soil added with the microbial inoculum is obviously higher than that of a control group, and is respectively 4.5 times and 2.5 times of that of the control group and that of the control group only added with wood vinegar, so that the content of the available phosphorus in the soil can be effectively improved by the strain YTR-M3.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Nanjing soil institute of Chinese academy of sciences
<120> Mesorhizobium strain having phosphate solubilizing property and use thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1341
<212>DNA
<213> Mesorhizobium sp
<400>1
tgcctccttg cggttagcgc agcgccttcg ggtaaagcca actcccatgg tgtgacgggc 60
ggtgtgtaca aggcccggga acgtattcac cgcggcatgc tgatccgcga ttactagcga 120
ttccaacttc atgcactcga gttgcagagt gcaatccgaa ctgagatggc ttttggagat 180
tagctcgacc tcgcggtctc gctgcccact gtcaccacca ttgtagcacg tgtgtagccc 240
agcccgtaag ggccatgagg acttgacgtc atccccacct tcctctcggc ttatcaccgg 300
cagtcccctt agagtgccca actgaatgat ggcaactaag ggcgagggtt gcgctcgttg 360
cgggacttaa cccaacatct cacgacacga gctgacgaca gccatgcagc acctgtcacc 420
ggtccagccg aactgaagga taccatctct ggtaaccgcg accgggatgt caagggctgg 480
taaggttctg cgcgttgctt cgaattaaac cacatgctcc accgcttgtg cgggcccccg 540
tcaattcctt tgagttttaa tcttgcgacc gtactcccca ggcgggaagc ttaatgcgtt 600
agctgcgcca ccgacaagta aacttgccaa cggctagctt ccatcgttta cggcgtggac 660
taccagggta tctaatcctg tttgctcccc acgctttcgc acctcagcgt cagtaccgga 720
ccagtgagcc gccttcgcca ctggtgttcc tccgaatatc tacgaatttc acctctacac 780
tcggaattcc actcacctct tccggactct agactaccag tatcaaaggc agttccgggg 840
ttgagccccg ggatttcacc cctgacttaa tagtccgcct acgtgcgctt tacgcccagt 900
aattccgaac aacgctagcc cccttcgtat taccgcggct gctggcacga agttagccgg 960
ggcttcttct ccggttaccg tcattatctt caccggtgaa agagctttac aaccctaggg 1020
ccttcatcac tcacgcggca tggctggatc aggcttgcgc ccattgtcca atattcccca 1080
ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggctg atcatcctct 1140
cagaccagct atggatcgtc gccttggtag gccattaccc caccaactag ctaatccaac 1200
gcgggctcat ccatctccga taaatctttc tccattagga cgtatacggt attagtccaa 1260
gtttccctgg gttattccgt agagatgggt agattcccac gcgttactca cccgtctgcc 1320
gctccccttg cggggcgctc g 1341
Claims (4)
1. A phosphate solubilizing intermediate rhizobium genus (A)MesorhizobiumSp) strain, characterized in that: the strain is rhizobium intermedium strain YTR-M3 which is preserved in China general microbiological culture collection center in 2019 in 4 and 9 months with the preservation number of CGMCC No. 17532.
2. A pharmaceutical composition comprising the intermediate rhizobium species of claim 1 (I), (II), (III)MesorhizobiumSp) microbial agents of the strain.
3. Mesorhizobium species (m) of claim 1MesorhizobiumSp) use of the strain for the decomposition of aluminium phosphate, iron phosphate, lecithin.
4. Use according to claim 3, characterized in that: the content of soluble sugar and soluble protein in the corn leaves and the biomass of underground parts of the overground part can be increased by matching the rhizobium intermedium strain YTR-M3 with wood vinegar.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101774864B (en) * | 2010-01-28 | 2011-02-16 | 南京信息工程大学 | Special seed coat eco-fertilizer for planting peanuts in red soil and preparation method and application thereof |
| CN103695335B (en) * | 2013-12-04 | 2015-05-27 | 中国农业科学院农业资源与农业区划研究所 | Mesorhizobium huakuii and use thereof |
| CN105733954A (en) * | 2015-12-18 | 2016-07-06 | 沈阳农业大学 | Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof |
| CN104673729B (en) * | 2015-03-23 | 2017-10-31 | 河南工业大学 | One plant of phosphorus decomposing rhizobium and its application combined in the endogenous phosphorus load of deposit is reduced with submerged plant |
| CN107881130A (en) * | 2017-11-10 | 2018-04-06 | 黑龙江省农业科学院草业研究所 | Rhizobium and its application with efficient phosphate-solubilizing ability |
| CN108239610A (en) * | 2016-12-26 | 2018-07-03 | 北京有色金属研究总院 | One plant of low temperature resistant phosphate solubilizing bacteria and its method for carrying out farmland phosphorus decomposing |
| CN105274030B (en) * | 2015-11-12 | 2018-10-12 | 领先生物农业股份有限公司 | A kind of rhizobium and application thereof |
| CN109097305A (en) * | 2018-08-28 | 2018-12-28 | 西安文理学院 | A kind of rhizobium and its application |
| CN106318889B (en) * | 2016-08-31 | 2019-10-08 | 云南磷化集团有限公司 | A kind of Enterobacter bacterial strain and its application with dissolving P capacity |
-
2019
- 2019-11-15 CN CN201911119326.5A patent/CN110713953B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101774864B (en) * | 2010-01-28 | 2011-02-16 | 南京信息工程大学 | Special seed coat eco-fertilizer for planting peanuts in red soil and preparation method and application thereof |
| CN103695335B (en) * | 2013-12-04 | 2015-05-27 | 中国农业科学院农业资源与农业区划研究所 | Mesorhizobium huakuii and use thereof |
| CN104673729B (en) * | 2015-03-23 | 2017-10-31 | 河南工业大学 | One plant of phosphorus decomposing rhizobium and its application combined in the endogenous phosphorus load of deposit is reduced with submerged plant |
| CN105274030B (en) * | 2015-11-12 | 2018-10-12 | 领先生物农业股份有限公司 | A kind of rhizobium and application thereof |
| CN105733954A (en) * | 2015-12-18 | 2016-07-06 | 沈阳农业大学 | Penicillium oxalicum (fungus) capable of efficiently dissolving phosphorus, culture method and applications thereof |
| CN106318889B (en) * | 2016-08-31 | 2019-10-08 | 云南磷化集团有限公司 | A kind of Enterobacter bacterial strain and its application with dissolving P capacity |
| CN108239610A (en) * | 2016-12-26 | 2018-07-03 | 北京有色金属研究总院 | One plant of low temperature resistant phosphate solubilizing bacteria and its method for carrying out farmland phosphorus decomposing |
| CN107881130A (en) * | 2017-11-10 | 2018-04-06 | 黑龙江省农业科学院草业研究所 | Rhizobium and its application with efficient phosphate-solubilizing ability |
| CN109097305A (en) * | 2018-08-28 | 2018-12-28 | 西安文理学院 | A kind of rhizobium and its application |
Non-Patent Citations (2)
| Title |
|---|
| Effect of indigenous Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture;Jay Prakash Verma 等;《Ecological Engineering》;20130108;第282-286页 * |
| 具有解磷能力的相思根瘤菌的筛选;张希涛 等;《林业科学研究》;20081015;第21卷(第5期);第619-624页 * |
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