CN109182194B - Rhizobium oridonii for promoting growth of corolla dentiger and culture method and application thereof - Google Patents
Rhizobium oridonii for promoting growth of corolla dentiger and culture method and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a rhizobium nodosum for promoting growth of corolla arachidis hypogaeae, and a culture method and application thereof. The strain number of the rhizobium oridonii is cv8401, and the preservation number of the rhizobium oridonii in the common microorganism center of China Committee for culture Collection of microorganisms is CGMCC No. 15532. Experiments prove that after the corolla coronaria is inoculated with the rhizobium japonicum cv8401 CGMCC No.15532, the plant nodulation rate, the plant height, the hay yield and the seed yield are obviously increased compared with those of a non-inoculated control. The invention has wide application prospect in the leguminous plant planting industry.
Description
Technical Field
The invention relates to a rhizobium populi for promoting growth of corolla arachnids in the field of agricultural microorganisms, and a culture method and application thereof.
Background
Corolla variabilis (Coronilla varira) is a perennial herb of the genus corolla of the family Leguminosae, is creeping and bushy, has dense branches and leaves and strong stress resistance, is a better covering green manure, and is also a valuable high-protein forage grass for ruminants. The corolla has developed root system, developed roots except the main roots, can transversely run in a soil layer, root system with root nodules, strong nitrogen fixation capacity, high breeding rate, luxuriant plants and high grass yield, can cut grass for three times every year in general cultivated land, has high coverage, can quickly form turf, and is a good plant for keeping water and soil. The small corolla can adapt to the north of the Yangtze river and the wide area of the south of the Yangtze river in China and cross the Yangtze river within a certain range. The corolla coronaria can not only fertilize soil and improve soil, but also beautify environment and reduce pollution.
Nitrogen is an important nutrient element in plant growth, crop production increases by applying nitrogen fertilizers are actually achieved at the cost of energy consumption and environmental pollution, and the process of converting nitrogen in the air into ammonia which can be absorbed by plants by nitrogen-fixing microorganisms in soil is efficient and harmless to the environment. Rhizobia (rhizobia) is a group of bacteria widely present in soil, which can form symbiotic relationship with leguminous plants, and transform nitrogen in the air into high-quality nitrogen-glutamic acid and glutamine substances which can be absorbed and utilized by plants in the form of symbiont by infecting roots or stems of leguminous plants to form root nodules. The study and use of legume rhizobia has been known for over a hundred years and the inoculation of legume crops with rhizobia has been advocated almost worldwide. The symbiotic system of the rhizobia and the leguminous plants is one of the systems with the strongest nitrogen fixing capacity of the known organisms, the annual nitrogen fixing amount accounts for more than 65 percent of the total nitrogen fixing amount of the organisms all over the world, and the potential for developing and utilizing the symbiotic nitrogen fixing effect of the leguminous plants and the rhizobia is huge.
Disclosure of Invention
The technical problem to be solved by the invention is how to obviously promote the growth and/or the yield increase of the corolla coronaria.
In order to solve the technical problems, the invention provides a rhizobium strain.
The rhizobia provided by the invention is Rhizobium yanglingense (Rhizobium yanglingense), the strain number of the Rhizobium yanglingense is cv8401, the strain is preserved in China general microbiological culture Collection center (CGMCC for short, the address is No. 3 of Xilu No.1 on North Chen of the sunward area in Beijing City) in 2018 for 30 days 03, the preservation number is CGMCC No.15532, and the strain is abbreviated as the Cv8401 of the Rhizobium yanglingense in the following.
The Rhizobium populi cv8401 has a 16s rDNA sequence shown in a sequence 1 in a sequence table.
A culture of Rhizobium populi cv8401 is also within the scope of the invention.
The culture of the Rhizobium nodosum cv8401 provided by the invention can be all substances in a culture container obtained by culturing the Rhizobium nodosum cv8401 in a microbial culture medium. The culture includes metabolites of the Rhizobium nodorum cv8401 and the Rhizobium nodorum cv 8401.
In the above culture of Rhizobium populi cv8401, the culture medium for the microorganism may be a solid medium or a liquid medium.
In order to solve the technical problems, the invention also provides a microbial inoculum for promoting the growth and/or yield increase of leguminous plants.
The microbial inoculum for promoting the growth and/or increasing the yield of leguminous plants, which is provided by the invention, contains a culture of the Rhizobium nodosum cv8401 or/and the Rhizobium nodosum cv 8401.
In the microbial inoculum, the promotion of the growth of the leguminous plants and/or the increase of the yield of the leguminous plants can be specifically the improvement of the plant height of the leguminous plants and/or the improvement of the yield of the hay of the leguminous plants and/or the improvement of the seed yield of the leguminous plants.
The active ingredient of the microbial inoculum can be a culture of the Rhizobium nodosum cv8401 or/and the Rhizobium nodosum cv8401, the active ingredient of the microbial inoculum can also contain other biological ingredients or non-biological ingredients, and other active ingredients of the microbial inoculum can be determined by the technical personnel according to the promoting effect of the microbial inoculum on the plant height of leguminous plants and/or the hay yield of leguminous plants and/or the seed yield of leguminous plants.
The microbial inoculum may also include a carrier. The carrier may be a solid carrier or a liquid carrier. The solid carrier is a mineral material or a biological material; the mineral material may be at least one of grass peat, clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the biological material is at least one of straws, pine shells, rice straws, peanut shells, corn flour, bean flour, starch, grass peat and animal manure of various crops; the liquid carrier can be water; in the preparation, the culture of Rhizobium nodosum cv8401 or/and Rhizobium nodosum cv8401 can be in the form of cultured living cells, a fermentation broth of the living cells, a filtrate of the cell culture, or a mixture of the cells and the filtrate. The preparation formulation of the microbial inoculum can be various preparation formulations, such as liquid, emulsion, suspending agent, powder, granules, wettable powder or water dispersible granules.
According to the requirement, the microbial inoculum can also be added with a surfactant (such as Tween 20, Tween 80 and the like), a binder, a stabilizer (such as an antioxidant), a pH regulator and the like.
The application of the culture of the Rhizobium oridonii cv8401 and the Rhizobium oridonii cv8401 or the microbial inoculum for promoting the growth and/or the yield increase of leguminous plants in preparing products for promoting the growth and/or the yield increase of the leguminous plants and the application in promoting the growth and/or the yield increase of the leguminous plants belong to the protection scope of the invention.
In the application, the promoting of the growth of the leguminous plants and/or increasing the yield of the leguminous plants can be specifically increasing the plant height of the leguminous plants and/or increasing the dry grass yield of the leguminous plants and/or increasing the seed yield of the leguminous plants.
In the application, the product for promoting the growth and/or the yield of the leguminous plants can be a microbial inoculum for promoting the growth and/or the yield of the leguminous plants or a biological fertilizer containing the microbial inoculum for promoting the growth and/or the yield of the leguminous plants.
In the present application, the leguminous plant may be a plant of the genus coronaria, such as coronaria.
In order to solve the technical problems, the invention also provides a method for culturing the Rhizobium populi cv 8401.
The method for culturing the Rhizobium nodulation strain cv8401 provided by the invention comprises the step of culturing the Rhizobium nodulation strain cv8401 in a culture medium for culturing the Rhizobium nodulation strain.
The culture medium for culturing rhizobia can be prepared according to the following method: 3-5ml of glycerol, 2-5g of mannitol, 0.8-1g of yeast extract powder and K2HPO40.5-1g, anhydrous MgSO4 0.1-0.2g，CaSO4·2H2O0.1-0.2 g, NaCl 0.1-0.2g, 1% by mass of (NH)4)6Mo7O24·4H21-1.5ml of O aqueous solution and 1% of H3BO31-1.5ml of aqueous solution and 20g of agar, adding water to a constant volume of 1000ml, adjusting the pH value to 6.8-7.0, and sterilizing to obtain the culture medium for culturing rhizobia.
In order to solve the technical problems, the invention also provides a method for preparing the Rhizobium populi cv8401 microbial inoculum.
The method for preparing the Rhizobium nodulation of the present invention cv8401 microbial inoculum comprises the step of taking the culture of the Rhizobium nodulation of the Rabdosia nodulation cv8401 and/or the Rhizobium nodulation of the Rabdosia nodulation cv8401 as the ingredient of the microbial inoculum.
Experiments prove that after the rhizobium populi cv8401 is inoculated to corolla coronaria, the nodulation rate, the plant height, the plant hay weight and the seed yield are obviously increased compared with those of an inoculation-free control: the nodulation rate is increased 326.47% compared with the non-inoculated contrast, the plant height is increased 106.03% compared with the non-inoculated contrast, the plant hay yield is increased 83.28% compared with the non-inoculated contrast, and the seed yield is increased 75.44% compared with the non-inoculated contrast. The invention has higher practicability and popularization, and is expected to be developed into a new excellent combination technology in the application field of the Rhizobium oridonii. The invention has wide application prospect in the planting industry of the corolla coronaria.
Deposit description
The strain name is as follows: rhizobium aspergilli (Rhizobium yanglingense)
The strain number is as follows: cv8401
The preservation organization: china general microbiological culture Collection center
The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)
Address: xilu No.1 Hospital No. 3 of Beijing market facing Yang district
The preservation date is as follows: 30.03 month in 2018
Registration number of the preservation center: CGMCC No.15532
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Rhizobium (Rhizobium sp.) ACCC19628 in the following examples was collected at the agricultural microorganism center of China Committee for culture Collection of microorganisms (abbreviated as ACCC, address: southern Avenue 12 of Guancun, in Hakken zone, Beijing, China academy of agricultural sciences, institute of agricultural resources and agricultural divisions, postal code 100081) on 1987 at 2.1.nd.. Hereinafter referred to as rhizobium ACCC 19628.
The preparation method of the solid medium in the following examples is as follows: 5ml of glycerol, 5g of mannitol and 1g of yeast extract powder, K2HPO40.5g, anhydrous MgSO4 0.2g，CaSO4·2H2O0.2 g, NaCl 0.1g, 1% by mass of (NH)4)6Mo7O24·4H21ml of O aqueous solution and 1 percent of H by mass3BO31ml of aqueous solution and 20g of agar, adding water to a constant volume of 1000ml, adjusting the pH value to 6.8-7.0, and sterilizing at 121 ℃ for 30 min.
The liquid medium in the following examples was prepared as follows: 5ml of glycerol, 5g of mannitol and 1g of yeast extract powder, K2HPO40.5g, anhydrous MgSO4 0.2g，CaSO4·2H2O0.2 g, NaCl 0.1g, 1% by mass of (NH)4)6Mo7O24·4H2O solution 1ml and H with mass content of 1%3BO31ml of the aqueous solution, adding water to a constant volume of 1000ml, adjusting the pH value to 6.8-7.0, and sterilizing at 121 ℃ for 30 min.
Example 1 isolation and characterization of Rhizobium yanglingense cv8401 CGMCC No.15532
1. Isolation of the Strain
Collecting corolla nodule in Shaanxi province, taking plate (10 g of mannitol, 1g of yeast extract powder, 0.5g of dipotassium hydrogen phosphate, 0.2g of calcium sulfate, 0.2g of magnesium sulfate, 0.1g of sodium chloride, 1ml of ammonium molybdate aqueous solution with the mass content of 1 percent, 1ml of boric acid aqueous solution with the mass content of 1 percent, 1ml of congo red with the mass content of 0.5 percent, 20g of agar, using water to fix the volume to 1L, and obtaining the strain cv8401 by streaking separation.
2. Identification of strains
2.1 morphological identification
And (3) performing single colony state description on the strain cv8401 which is in a logarithmic growth phase and has stable colony size and is separated and purified in the step 1, wherein the single colony state description mainly comprises the size, color, transparency, wettability, colony surface state and colony edge state of the colony. On the other hand, the strain cv8401 in the logarithmic growth phase was stained by a smear, and the morphology of the cells was observed by an optical microscope.
The result shows that the cv8401 strain has a short and rod-shaped thallus, the size of the thallus is 0.5-0.9 micrometer multiplied by 1.2-3.0 micrometers, and the thallus forms grown in different culture media are different. Containing granules of poly-beta-hydroxybutyrate. Gram negative, no spore, flagellum, sports and aerobic. The optimum growth temperature is 25-30 ℃, and the optimum pH value is 6.0-9.0. The bacterial colony is round, neat in edge, convex, colorless, translucent or light milky white, and viscous. Congo red does not absorb color when cultured, and the growth on sugar-containing medium is often accompanied by abundant extracellular mucus.
2.2, 16S rDNA sequence homology analysis
The 16S rDNA fragment of the strain cv8401 obtained in step 1 was amplified by colony PCR, and the reagents were provided by Takara Shuzo Co. The results of amplifying and cloning sequencing the 16S rRNA gene fragment show that the 16S rDNA of the strain cv8401 has a nucleotide sequence of a sequence 1 in a sequence table. The similarity of the 16S rDNA of the strain cv8401 to the 16S rRNA gene of Rhizobium yanglingense (Sequence ID: JF273845.1) of Rhizobium japonicum is 100% at the highest.
2.3, physiological and biochemical characterization
The physiological and biochemical characteristics of the strain cv8401 were determined by reference to "handbook of identification of common bacteria system" (Dongxu bead, Chuiamiao Ying. handbook of identification of common bacteria system. Beijing: scientific Press, 2011) and "experiments in microbiology" (Shen Nu, Fang Xiu, Li Guangwu. microbiological experiments (third edition). Beijing: advanced education Press, 1999.) "Bergey Manual of identification of bacteria" eighth edition (R.E. Bukanan, N.E. Gibens et al eds. scientific Press, 1984.). The result shows that the strain cv8401 is chemoheterotrophic, can utilize various carbohydrates and salts of organic acids as carbon sources, such as glucose, lactose, D-ribose, D-arabinose, mannitol, maltose, D-galactose, fructose, trehalose and inositol; peptone, yeast juice, glutamic acid, ammonium salt, nitrate and most amino acids can be used as nitrogen sources; slightly absorbing color by congo red color absorption reaction; the litmus milk reacts to produce acid and has a serum ring; BTB produces acid; the casein cannot be hydrolyzed; 3-ketolactose is not utilized; producing acid from mannitol; growth on sugar-containing media is often accompanied by abundant extracellular mucus; no precipitation was produced in calcium glycerophosphate medium.
The strain cv8401 isolated and purified in step 1 was identified as Rhizobium yanglingense (Rhizobium yanglingense) in view of the above morphological, physiological and biochemical characteristic analysis and 16s rDNA sequence homology analysis results. The Rhizobium yanglingense cv8401 is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 of Xilu No.1 of Beijing Kogyo area of the Chaoyang area) in 2018, 03 and 30 days, and the preservation number is CGMCC No.15532, which is hereinafter referred to as the Rhizobium cyrilensis cv8401 for short.
Example 2 preparation of Rhizobium nodosum cv8401 microbial inoculum for promoting growth of corolla arachidis hypogaeae
1. Slant culture of Rhizobium populi cv8401
Inoculating the Rhizobium nodosum cv8401 to a solid culture medium for slant culture, and culturing at 28 ℃ for 72 hours to obtain the Rhizobium nodosum cv8401 by slant culture.
2. Activation of bacterial species
And (3) selecting the rhizobium populinum cv8401 which is cultured in the inclined plane in the step (1), inoculating the rhizobium populinum cv8401 into 500ml of liquid culture medium, and culturing for 60h at the temperature of 28 ℃ to obtain the rhizobium populinum cv8401 bacterial liquid.
3. Seeding tank culture
Taking 6L of the Rhizobium nodosum cv8401 bacterial liquid obtained in the step 2, inoculating the bacterial liquid into a 100L seed tank (containing 60L of liquid culture medium) for seed culture, and performing shaking culture at 28 ℃ and 150rpm for 60h to obtain the Rhizobium nodosum cv8401 seed liquid.
4. Cultivation in fermenter
Inoculating the seed solution of Rhizobium nodosum cv8401 obtained in the step 3 into a 1000L fermentation tank (containing 600L of liquid culture medium) according to the proportion (volume ratio) of 10 percent for fermentation culture, and performing shaking culture at 28 ℃ and 150rpm for 60 hours to obtain the Rhizobium nodosum cv8401 fermentation liquor, wherein the content of cv8401 in the Rhizobium nodosum fermentation liquor is 2 multiplied by 109cfu/mL。
5. Preparation of Rhizobium nodosum cv8401 microbial inoculum
Naturally drying the grass peat, then crushing, sieving with a 100-mesh sieve, then adjusting the pH value to 6.8 with lime water, and sterilizing at 121 ℃ for 60min to obtain the sterile grass peat.
And (4) uniformly mixing the rhizobium nodosum cv8401 fermentation liquor obtained in the step (4) with the sterile grass carbon, performing enrichment culture at the temperature of 28 ℃ for 48 hours to obtain a rhizobium nodosum cv8401 microbial inoculum, and subpackaging and warehousing. The product is qualified, and the cv8401 bacteria content in the nodulation of the Rhizobium populi cv8401 bacteria is 2.0 multiplied by 108cfu/g。
Example 3 comparison of Effect of Rhizobium yanglingense cv8401 CGMCC No.15532 on the inoculation of coronaria miniata with Rhizobium accC19628
The following tests were carried out using the Rhizobium ACCC19628 isolated by the applicant from corolla florida rhizomas in Shaanxi province of China as a control:
preparation of rhizobium ACCC19628 microbial inoculum
1. Slant culture
Rhizobium ACCC19628 was inoculated into a solid medium and cultured on a slant at 28 ℃ for 72 hours.
2. Activation of bacterial species
And (3) selecting the rhizobium ACCC19628 cultured on the slant in the step (1) to inoculate in 500mL of liquid culture medium, and culturing at 28 ℃ for 60h to obtain a rhizobium ACCC19628 bacterial liquid.
3. Seeding tank culture
And (3) taking 6L of the rhizobium ACCC19628 bacterial liquid obtained in the step (2), inoculating the rhizobium ACCC19628 bacterial liquid into a 100L seed tank (containing 60L of liquid culture medium) for seed culture, and performing shaking culture at 30 ℃ and 120rpm for 60h to obtain a rhizobium ACCC19628 seed solution.
4. Cultivation in fermenter
Inoculating the rhizobium ACCC19628 seed solution obtained in the step 3 into a 1000L fermentation tank (containing 600L of liquid culture medium) according to a proportion (volume ratio) of 10% for fermentation culture, and performing shaking culture at 28 ℃ and 150rpm for 60h to obtain a rhizobium ACCC19628 fermentation liquor.
5. Preparation of Rhizobium coronarium (Rhizobium sp.) ACCC19628 microbial agent
Naturally drying the grass peat, then crushing, sieving with a 100-mesh sieve, then adjusting the pH value to 6.8 with lime water, and sterilizing at 121 ℃ for 60min to obtain the sterile grass peat.
And (4) uniformly mixing the rhizobium ACCC19628 fermentation liquor obtained in the step (4) with the sterile grass peat, performing proliferation culture at the temperature of 28 ℃ for 48 hours to obtain a rhizobium ACCC19628 microbial inoculum, and subpackaging and warehousing. The finished product is qualified by detection, and the effective viable count content in the rhizobium ACCC19628 microbial inoculum is 2.0 multiplied by 108cfu/g。
Second, field effect test for promoting growth of small-crown peanuts
The test site is in Tongliao city in inner Mongolia autonomous region. The test adopts a random block design, three treatment areas are randomly arranged, and each treatment area is provided with three cell repetitions. The three treatment areas are respectively a non-inoculated bacteria control treatment area, a cv8401 microbial inoculum treatment area and an ACCC19628 microbial inoculum treatment area. Each cell size is 4 x 5m2The pH of the soil was 7.6.
The rabdosia populi cv8401 microbial inoculum prepared in example 2 and corolla coronaria seeds were mixed according to the ratio of 1: 10, and the obtained seeds are called cv 8401-corolla coronaria seeds.
And (3) mixing the rhizobium ACCC19628 microbial inoculum prepared in the step one with the corolla minoxidil seeds according to the proportion of 1: 10, and the obtained seeds are called ACCC 19628-corolla erecta seeds.
Mixing the sterile grass peat and the small crown flower seeds according to the proportion of 1: 10, and the obtained seeds are called as sterile grass peat-corolla minutissima seeds.
The seeds are uniformly sown in the soil according to the cell design, the sowing method is drill sowing, and the row spacing is 10 cm. The conditions in the above treatment zones are identical except for the different seeds to be sown. Wherein, the seeds sowed in the non-inoculation control treatment area are aseptic grass peat-corolla coronaria seeds; the seed sowed in the cv8401 microbial inoculum processing area is cv 8401-corolla coronaria seed; the seeds sowed in the ACCC19628 microbial inoculum treatment area are ACCC 19628-corolla erecta seeds.
When the seeds are mature in the current year and the seeds are mature in the next year, the underground nodule rate (for short), the height of the overground part plants, the hay yield and the seed yield are respectively recorded, the average value of each group is calculated, and the average nodule rate, the average height of the overground part plants (for short, the average height of the plants), the average hay yield and the average seed yield in two years are obtained.
TABLE 1 test results of inoculating corolla coronaria with Cv8401 inoculum of Rhizobium oridonii and ACCC19628 inoculum of Rhizobium oridonii
The results are shown in table 1 and indicate:
1. compared with the corolla florida in the non-inoculated control treatment area, the average tumor formation rate of the corolla florida in the cv8401 fungicide treatment area is increased by 326.47% in two years, the average plant height is increased by 106.03% in two years, the average hay yield is increased by 83.28% in two years, and the average seed yield is increased by 75.44% in two years.
2. compared with the corolla in the ACCC19628 microbial inoculum treatment area, the average tumor forming rate of the corolla in two years is increased by 81.25%, the average plant height in two years is increased by 46.59%, the hay yield in two years is increased by 48.40%, and the average seed yield in two years is increased by 42.57%.
The results show that the Rhizobium populi cv8401 disclosed by the invention has more remarkable growth promoting and yield increasing effects on corolla coronaria than the Rhizobium ACCC 19628.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
<110> institute of agricultural resources and agricultural regionalism of Chinese academy of agricultural sciences
<120> Rhizobium oridonii for promoting growth of corolla arachidis hypogaeae, and culture method and application thereof
<130> GNCFH182036
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1372
<212> DNA
<213> Rhizobium yanglingense (Rhizobium yanglingense)
<400> 1
agggggcgac tacacatgca agtcgagcgc cccgcaaggg gagcggcaga cgggtgagta 60
acgcgtggga acgtaccctt tactacggaa taacgcaggg aaacttgtgc taataccgta 120
tgtgcccttc gggggaaaga tttatcggta agggatcggc ccgcgttgga ttagctagtt 180
ggtggggtaa aggcctacca aggcgacgat ccatagctgg tctgagagga tgatcagcca 240
cattgggact gagacacggc ccaaactcct acgggaggca gcagtgggga atattggaca 300
atgggcgcaa gcctgatcca gccatgccgc gtgagtgatg aaggccctag ggttgtaaag 360
ctctttcacc ggagaagata atgacggtat ccggagaaga agccccggct aacttcgtgc 420
cagcagccgc ggtaatacga agggggctag cgttgttcgg aattactggg cgtaaagcgc 480
acgtaggcgg acatttaagt caggggtgaa atcccagagc tcaactctgg aactgccttt 540
gatactgggt gtctggagta tggaagaggt gagtggaatt ccgagtgtag aggtgaaatt 600
cgtagatatt cggaggaaca ccagtggcga aggcggctca ctggtccatt actgacgctg 660
aggtgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 720
atgaatgtta gccgtcggca agtttacttg tcggtggcgc agctaacgca ttaaacattc 780
cgcctgggga gtacggtcgc aagattaaaa ctcaaaggaa ttgacggggg cccgcacaag 840
cggtggagca tgtggtttaa ttcgaagcaa cgcgcagaac cttaccagcc cttgacatgc 900
ccggctacct acagagatgt agggttccct tcggggaccg ggacacaggt gctgcatggc 960
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aaccctcgcc 1020
cttagttgcc agcattcagt tgggcactct aaggggactg ccggtgataa gccgagagga 1080
aggtggggat gacgtcaagt cctcatggcc cttacgggct gggctacaca cgtgctacaa 1140
tggtggtgac agtgggcagc gagcacgcga gtgtgagcta atctccaaaa gccatctcag 1200
ttcggattgc actctgcaac tcgagtgcat gaagttggaa tcgctagtaa tcgcggatca 1260
gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt 1320
tggttttacc cgaaggtagt gcgctaaccg caaggaggca gctaacctcg ag 1372
Claims (6)
1. Rhizobium yanglingense (Rhizobium yanglingense) has the strain number cv8401 and the preservation number of CGMCC No.15532 in China general microbiological culture Collection center.
2. The microbial inoculum for promoting the growth and/or the yield increase of the coronaria plant is characterized in that: the microbial preparation comprises the Rhizobium japonicum of Rabdosia rubescens of claim 1.
3. Use of the rhizobium popsicum of claim 1 or the inoculant of claim 2 for the preparation of a product for promoting the growth and/or production of corolla arachidis hypogaeae.
4. Use of the rhizobium japonicum of slush according to claim 1 or the inoculant of claim 2 for promoting the growth and/or production of peanut microcrowns.
5. A method of culturing Rhizobium nodulation of Rabdosia rubescens of claim 1, comprising the step of culturing the Rhizobium nodulation of claim 1 in a medium for culturing Rhizobium.
6. A method for producing the microbial preparation of claim 2, comprising the step of using the Rhizobium japonicum of claim 1 as a component of the microbial preparation.
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| CN110818500A (en) * | 2019-09-16 | 2020-02-21 | 甘肃农业大学 | Special bacterial fertilizer for sainfoin and preparation method thereof |
| CN114045240B (en) * | 2021-11-18 | 2023-07-14 | 中国林业科学研究院森林生态环境与保护研究所 | Rhizobium, microbial inoculum prepared from rhizobium and application of rhizobium |
| CN115287229B (en) * | 2022-08-04 | 2023-05-12 | 兰州大学 | Rhizobium capable of promoting growth of alfalfa beans and nodulation and nitrogen fixation and application thereof |
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