CN111019660A - Microbial soil conditioner and preparation method and application thereof - Google Patents
Microbial soil conditioner and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial soil conditioner and a preparation method and application thereof. The microbial soil conditioner is obtained by adding 10-15% of functional strain fermentation liquor into a matrix conditioner and mixing the matrix conditioner mixed with the fermentation liquor with soil; the functional strains are selected from Trichoderma harzianum with a strain number of BNCC 336568, Pseudomonas fluorescens with a strain number of BNCC 231887 and one or more of Streptomyces griseus with a strain number of BNCC 335914, the matrix modifier is a mixture of vinegar residue and rice straw charcoal, and the volume ratio of the vinegar residue to the rice straw charcoal is 1: 0.8-1.2; the volume ratio of the matrix modifier to the soil is 1: 23-28. The invention utilizes the soil local microorganisms and the exogenous beneficial microorganisms to jointly act on the matrix conditioner in a coordinated manner, thereby achieving the effects of balancing the soil microorganism environment, reducing the influence of fungal diseases and simultaneously improving the soil physical and chemical environment.
Description
Technical Field
The invention belongs to the field of soil conditioning materials, and relates to a microbial soil conditioner, and a preparation method and application thereof.
Background
In recent 30 years, facility agriculture in China has been rapidly developed and is the country with the largest facility cultivation area in the world. In agricultural production, environmental conditions such as crop diseases, salt stress, acidification and the like are the most important factors for restricting the yield and the quality of horticultural crops. However, the production of horticultural crops at home and abroad currently mainly uses a large amount of chemical fertilizers to promote the growth of crops, and then uses a large amount of chemical pesticides to prevent and control plant diseases and insect pests as auxiliary materials to obtain great benefits in yield. This model has brought about a great economic investment in the long run, and the use of chemical fertilizers and agricultural chemicals in large quantities has caused serious environmental problems in spite of the great increase in yield of horticultural crops. Meanwhile, the development of scale, specialization and industrialization of facility vegetable production also leads to the increasingly serious continuous cropping obstacles (which refer to the phenomena of slow growth, poor development, weak growth, reduced yield and quality, plant diseases and insect pests and the like in different degrees even under normal cultivation and management conditions when the same kind or same family crops are continuously planted on the same soil) in some areas, thereby limiting the sustainable development of vegetable production and the increasing economic benefit in some areas. Thus, environmentally friendly, sustainable agricultural practices are becoming increasingly popular and appreciated. A number of studies have clearly pointed out that the factors leading to the development of continuous cropping obstacles are mainly: (1) the facility cultivation crops lack rainwater leaching, a large amount of basic ions are attached to the soil surface, and nutrients are lost; (2) the application of a large amount of chemical fertilizers and pesticides causes the imbalance of the physical and chemical environment of soil, the activation of latent pathogens and the destruction of the physiological metabolism of plants; (3) the plant autotoxicity and the root system secretes organic micromolecules such as phenolic acids and the like, so that the balance of the soil ecological environment is damaged, and the nutrient absorption of the plant is influenced.
At present, the method for preventing and treating the continuous cropping obstacle of the soil at home and abroad mainly aims at reducing the application of pesticides and fertilizers, reasonably rotating crops, selectively breeding disease-resistant materials of horticultural crops and applying physical soil conditioners to improve the habitat of the soil and promote the growth of the horticultural crops. The most applied to improving the continuous cropping obstacle soil is mainly to change a cultivation system, reduce physical regulation aspects such as multiple cropping indexes and the like, and recently, the research center of gravity has been shifted to chemical and biological improvement aspects in slow production research: in the chemical aspect, the physical and chemical environment of the obstacle soil is changed to a certain extent mainly by applying certain organic or inorganic substrates (such as biochar, vinegar residue, mushroom residue, cassava residue and the like), firstly, the pH value of the obstacle soil is improved, the salt content is reduced, secondly, the nutrient environment of the obstacle soil is regulated and controlled, and the quick-acting nutrient content (such as available nitrogen, phosphorus and potassium) of the soil is effectively improved; in the biological aspect, soil microorganisms are mainly associated with the activity of related enzymes, and the soil microorganisms and the related enzymes cooperate to regulate and control the biological environment of soil, thereby laying a foundation for physicochemical environment. Both solve a series of problems caused by the continuous cropping obstacles of the soil to a certain extent, but are incomplete and comprehensive.
The current mainstream research method for soil conditioners is generally as follows: uniformly mixing the substrate with the obstacle soil according to a certain application proportion; the technical research is mainly carried out by potted planting crops (cucumber, tomato, peanut and the like) to supply normal water and fertilizer; the soil conditioner is comprehensively evaluated whether to be applicable or not by observing and surveying the salinity, the pH value and the available nutrients of the soil and the growth of crops. Such a research method has the following drawbacks: (1) the method is only limited to the research of physical and chemical properties, and the research of soil environment is incomplete; (2) the period for screening the novel soil conditioner is too long; (3) localized to a physical matrix, failing to associate with beneficial microorganisms; (4) the action mechanism of the novel matrix conditioner is not clear, and the function is incomplete; (5) the innovation point of the experimental research method is not enough, and the similar methods of predecessors are still used; (6) part of the substrates have higher cost and are troublesome to apply, the physicochemical environment of the obstacle soil can be improved only, and the action of local microorganisms is basically ignored; (7) the existing soil conditioner formula has short effect period for soil improvement and only meets the short-term effect; (8) the prior art has too single action object and limited function, only can be used for soil improvement, has no other additional beneficial function (9), is almost rarely applied to actual cultivation although most of the prior art determines a formula, and has poor effect and low benefit.
Disclosure of Invention
The invention aims to provide a microbial soil conditioner which can improve the physical and chemical environment of salinized continuous cropping soil, accumulate available nutrients of the soil to a certain extent and effectively regulate and control the microbial environment of the soil, aiming at the defects in the prior art.
The invention also aims to provide a preparation method of the microbial soil conditioner.
The invention also aims to provide application of the soil conditioner.
The purpose of the invention can be realized by the following technical scheme:
a microbial soil conditioner is prepared by adding functional strain fermentation liquor into a matrix conditioner according to the ratio of 10-15% (V/W), and mixing the matrix mixed with the fermentation liquor with soil; the functional strains are selected from Trichoderma harzianum with a strain number of BNCC 336568, Pseudomonas fluorescens with a strain number of BNCC 231887 and one or more of Streptomyces griseus with a strain number of BNCC 335914, the matrix is a mixture of vinegar residue and rice straw charcoal, and the volume ratio of the vinegar residue to the rice straw charcoal is 1: 0.8-1.2; the volume ratio of the matrix to the soil is 1: 23-28.
The microbial soil conditioner is preferably selected, and the bacterial concentration of the functional strain fermentation liquor is 1 x 107CFU/ml。
The fermentation liquor of the functional strain is preferably added into the substrate according to 10 percent (V/W)
The functional strain is preferably selected from streptomyces griseus with the strain number of BNCC 335914.
The mixing volume ratio of the preferable substrate of the microbial soil conditioner to the soil is vinegar residue: rice straw charcoal: the soil is 1: 1: 50.
the preparation method of the microbial soil conditioner is characterized by comprising the following steps of:
(1) liquid culturing the functional bacteria to obtain mature bacteria suspension;
(2) adjusting the concentration of the bacterial suspension by a method of counting by a blood counting plate, inoculating the bacterial suspension into the matrix, and mixing the matrix and the soil after uniform mixing.
The microbial soil conditioner disclosed by the invention is applied to reducing the salt content of the salinized soil and improving soil acidification.
The microbial soil conditioner disclosed by the invention is applied to accumulation of available nutrients of soil.
The microbial soil conditioner disclosed by the invention is applied to effectively regulating and controlling soil microbial environment.
The microbial soil conditioner disclosed by the invention is applied to reducing the salinity content of salinized soil, improving soil acidification, accumulating available soil nutrients and/or effectively regulating and controlling a soil microbial environment.
Has the advantages that:
the invention is a microbial soil conditioner with systematic and complete functions, which comprises three properties of soil physics, chemistry and biology; the method has the advantages that the connection and interaction between microorganisms and a matrix (such as vinegar residue, biochar and the like) are enhanced, and strong connection is achieved, so that the problem of singleness that only the matrix is used as the modifier is solved, beneficial microorganisms can act synergistically with the matrix to provide sufficient nutrient environment and micro-ecological environment for the cultivation and production of crops together, and the improvement efficiency is higher and more comprehensive, so that the novel microbial soil modifier disclosed by the invention is superior to the basic physicochemical improvement effect of the soil modifier in the prior art, and a function is developed on the basis to provide seedling production; the most main active ingredient in the preferred technical scheme of the invention is actinomycetemcomita-gray streptomyces which is less in production and application at present, and the invention finds that the actinomycetemcomita-gray streptomyces has a plurality of potential values, can be added into a seedling culture substrate, not only has the effect of accumulating nutrients, but also has the tendency of enriching beneficial microorganisms.
The invention takes the common and well-separated materials of organic wastes and microorganisms in industrial production as the technical support center of gravity, on one hand, the problem of recycling the wastes is solved, the utilization efficiency of the wastes is improved, on the other hand, the organic fertilizer or the organic seedling substrate can be used for seedling application, and the functionality of the soil conditioner is enriched.
The invention uses the low-cost organic waste vinegar residue, the material is easy to obtain and has potential nutritive value and biological research value, and the cost is reduced to a certain extent.
The invention is a novel soil conditioner of microorganism coupling matrix which can obviously improve the salinity of the salinized soil, adjust the acid-base balance, effectively accumulate or convert the available nutrients of the soil and balance the beneficial microbial community of the soil; the organic fertilizer can be used as a soil conditioner for improving continuous cropping obstacle soil and a seedling culture substrate for vegetable seedling culture, which means that the organic fertilizer not only has the functions of improving soil environment and killing fungi and recombining local microorganisms, but also is a provider of fertilizer or nutrient required by crop growth.
The invention has the advantages of low carbon, environmental protection, low cost, wide application, long activity period of microorganism (because of actinomycetes), good benefit and capability of providing long-term nutrient supply for crops.
Drawings
FIG. 1 is a technical scheme work flow chart
Note:
the same amount of sterile water as the added sterile agent is shown.
FIG. 2 regulating and controlling effect of different microbial matrix modifiers on soluble total salt content of soil
Note: wherein T1-T9 respectively represent the above TR-Trichoderma harzianum; Tv-Trichoderma viride; Pl-Paecilomyces lilacinus; Jc-Bacillus subtilis; Ba-Bacillus amyloliquefaciens; Bl-Bacillus licheniformis; pf pseudomonas fluorescens; sm-fine yellow streptomyces; Sg-Gray Streptomyces.
FIG. 3 the accumulation of available nutrients in soil by different microbial matrix modifiers
A accumulation effect of different microbial matrix modifiers on available nitrogen nutrients of soil
B accumulation of different microbial matrix modifiers on available phosphorus nutrients in soil
Note: wherein T1-T9 respectively represent the above TR-Trichoderma harzianum; Tv-Trichoderma viride; Pl-Paecilomyces lilacinus; Jc-Bacillus subtilis; Ba-Bacillus amyloliquefaciens; Bl-Bacillus licheniformis; pf pseudomonas fluorescens; sm-fine yellow streptomyces; Sg-Gray Streptomyces.
Detailed Description
Example 1
(1) The formula of the culture medium is as follows: the tested bacteria culture medium, beef extract 3g, peptone 10g, NaCl5.0g, agar 15.0g, distilled water 1.0L, pH7.0, 121 ℃, 15min high temperature sterilization; trichoderma harzianum (fungus), synthetic PDA-1.0L, KH of 20% potato cooking juice2PO43g，MgSO4·7H2O 1.5g，C6H12O620g of VB 110 mg and 20g of agar, wherein the pH is natural, and the sterilization is carried out at the high temperature of 121 ℃ for 15 min; green trichoderma (fungus) and streptomyces griseus (actinomycetes) -20% potato cooking juice 1.0L, C6H12O620g，KH2PO43g，MgSO4·7H2O1.5 g, VB 110 mg, agar 15g, natural pH, 121 ℃, and high-temperature sterilization for 15 min; streptomyces microflavus (Actinomycetes) -KNO31g of soluble starch, 20g of KH2PO40.5g，MgSO4·7H2O 0.5g，NaCl 0.5g，FeSO40.01g of agar, 20g of agar and 1.0L of distilled water, wherein the pH value is 7.2-7.4, the sterilization is carried out at the high temperature of 121 ℃ for 15 min; paecilomyces lilacinus (fungi), Czapek's-sucrose 30.0g, NaNO33.0g，MgSO4·7H2O 0.5g，KCl 0.5g，FeSO40.01g，K2HPO41.0g, agar 15.0g, and distilled water 1.0L, pH6.0-6.5, sterilizing at 121 deg.C for 15 min.
(2) The culture conditions are as follows: bacteria for 1-2 days, fungi for 2-3 days, and actinomycetes for 5-7 days in a biochemical incubator at 28 deg.C. When the complete bacterial colony is cultured in a biochemical incubator (bacteria for 1-2 days, fungi for 3 days and actinomycetes for 5 days), subculture is carried out on a new culture medium on an ultra-clean workbench to obtain mature bacterial strains, and further, each strain is inoculated into a 150ml conical flask filled with respective corresponding liquid culture medium, the shake flask culture parameters are set to be 200r/min, and shake culture is carried out for 24-48h to obtain mature bacterial suspension.
(3) Further, the concentration of the bacterial suspension was adjusted to 1 × 10 in a manner of counting the bacterial suspension on a hemocytometer7Inoculating the mixture into a matrix (rice straw charcoal: vinegar residue 1: 1) in a volume mass ratio of 10% after CFU/ml, uniformly mixing, and adding the mixture into the matrix: the soil is 1: mixing 25(v/v) in the soil with the soil.
(4) Test strains: trichoderma harzianum TR-BNCC 336568; trichoderma viride Tv-BNCC 187879; paecilomyces lilacinus Pl-BNCC 342068; bacillus amyloliquefaciens Ba-BNCC 195265; bacillus licheniformis Bl-BNCC 189067; pseudomonas fluorescens Pf-BNCC 231887; the streptomyces microflavus Sm-BNCC 337579; streptomyces griseus Sg-BNCC 335914, the remaining strains were laboratory preserved.
Example 2 improvement of salinity environment, reduction of salinity content of soil to some extent, and increase of pH of soil
(1) Soil culture test: mixing matrix materials of rice straw charcoal and vinegar residue in a ratio of 1: 1, and then the mature bacterial suspension (TR-Sg) obtained by the culture in example 1 was adjusted to a concentration of C1 × 10 with sterile water7After CFU/ml, inoculating the mixture into the mixed matrix according to the volume mass ratio of 10 percent, then uniformly mixing the microbial matrix conditioner and the obstacle soil according to the volume ratio of 1:25, and using a conventional greenhouseCulturing in a greenhouse for 30 days, and simultaneously detecting the chemical environment of the soil by taking 0-10-20day as research time.
(2) And (3) soil pH determination: a fresh soil sample 10g was taken, and the ratio of 5: 1, adding 50ml of distilled water, repeatedly oscillating for 30mi, standing for 10min, filtering with non-phosphorus filter paper, and taking supernatant to measure the pH value of soil leaching liquor by using a Spectrum PH 400Meter Item 2107.
(3) And (3) soil EC determination: a fresh soil sample 10g was taken, and the ratio of 5: 1, adding 50ml of distilled water, repeatedly oscillating for 30mi, standing for 10min, filtering with non-phosphorus filter paper, and measuring the EC value of the Soil leaching liquor by taking the supernatant with a Spectrum Direct Soil EC Meter Item2265 FS.
TABLE 2 Regulation and control of soil physicochemical environment by different microbial matrix improving agents
As can be seen from the data results in table 2 and fig. 2, through the addition of the microbial strains and the matrix, the facility greenhouse soil for perennial (continuous cropping 9 years) continuous cropping can significantly improve the soil acidity and alkalinity under the influence of the microbial strains, so that the soil is separated from the former acidification environment to a certain extent, and the more significant the effect is along with the extension of the culture time, the better the improvement effect is; meanwhile, the conductivity of the barrier soil is reduced to a certain extent, and the two directly determine a basic physicochemical environment for whether crops can grow well or even survive. Obviously, after the microbial matrix modifier is added, the physical and chemical environment of the salinized soil is obviously improved, and TR, Pl, Pf and Sg have more obvious regulation and control effects compared with other treatments.
Example 3 accumulating soil available nutrients to provide a good nutrient reserve for crop growth
(1) Soil culture test: mixing matrix materials of rice straw charcoal and vinegar residue in a ratio of 1: 1, and then culturing the mixture obtained in example 1The concentration of the mature bacterial suspension (TR-Sg) was adjusted to 1X 10 with sterile water7After CFU/ml, inoculating the mixture into the mixed matrix according to the volume mass ratio of 10%, then uniformly mixing the microbial matrix conditioner and the obstacle soil according to the volume ratio of 1:25, culturing in a conventional greenhouse for 30 days, and simultaneously detecting the available nitrogen and phosphorus nutrients of the soil by taking 0-10-20day as research time respectively.
(2) And (3) measuring available nitrogen nutrients of the soil: uniformly spreading 2g of an air-dried soil sample in a diffusion dish outer chamber, adding 1-2 drops of methyl red-bromocresol green mixed indicator into 2ml of 2% boric acid solution, uniformly mixing, coating alkaline glycerol on the edge of the outer chamber, and covering ground glass to rotate and seal tightly; slowly rotating the ground glass cover to expose the gap on one side of the outer chamber, adding 10ml of 1.0mol/L NaOH solution from the gap, immediately covering, carefully rotating the diffusion dish to fully mix the alkali liquor and the soil sample, binding with a rubber band, placing in a 40 ℃ thermostat for 2 hours, taking out, and titrating with 0.005mol/L sulfuric acid labeled solution until the color of the solution is changed from blue-green to reddish.
Hydrolyzable nitrogen (mg/kg) ═ V-V0 (V-V0) × 14 × 1000/m
In the formula: c is the concentration of the labeled titrating acid H +, mol/L;
v0 is the number of ml used for titration of the blank;
v is the number of milliliters used for titrating the sample;
14 is the millimolar mass of the nitrogen yard, g;
1000 is a coefficient converted to milligrams of nitrogen per kg of sample.
(3) And (3) determination of available phosphorus nutrients of soil: weighing 2.5g of 1mm air-dried soil sample, placing in a dry 150ml conical flask, adding 0.5mol/L of leaching solution, pH 8.5 NaHCO3Oscillating the solution at room temperature for 30min, filtering with non-phosphorus filter paper in a dry 150ml conical flask, sucking 10ml into the dry 150ml conical flask (sucking 2.5-5.0ml when the content is higher, and adding the extractive solution to 10ml), and adding 35ml distilled water; then adding 5ml of molybdenum-antimony color-developing resisting agent by using a liquid transfer gun, and shaking uniformly; standing for 30min, and performing color comparison with 700nm wavelength with blank as control.
Rho-mass concentration (mu g/mL) of P found from the working curve;
v is volume (mL) determined by volume during color development;
m-air drying mass (g);
ts-is the fractional multiple (i.e. the ratio of the total volume of the leach liquor to the volume of the leach liquor drawn);
k-the coefficient for converting the air drying into the quality of the drying matrix;
103-converting the mu g into mg;
1000-conversion to P content per kg
From fig. 3, it can be derived that: different microbial matrix improvers have different effects on the available nutrients of the soil after being applied to the obstacle soil. Compared with other microbial strain treatments, Pf and Sg have remarkable functions of accumulating available nitrogen and available phosphorus, provide a good nutrient environment for the growth of crops, ensure the supply of nutrients to a certain extent, and show a 'non-reduction' and 'level' state with the increase of the period, which means that the nutrient supply period can be prolonged.
Example 4 microbial Environment improvement and reduction of harmful fungus abundance
(1) Soil culture test: mixing matrix materials of rice straw charcoal and vinegar residue in a ratio of 1: 1, and then the mature bacterial suspension (TR-Sg) obtained by the culture in example 1 was adjusted to a concentration of C1 × 10 with sterile water7After CFU/ml, inoculating the mixture into the mixed matrix according to the volume mass ratio of 10%, then uniformly mixing the microbial matrix conditioner and the obstacle soil according to the volume ratio of 1:25, culturing for 30 days in a conventional greenhouse, and simultaneously detecting the abundance of the soil microbial community by taking 10-20day as research time.
(2) The formula of the culture medium is as follows: the beef extract slant culture medium is prepared by adding 1.5g beef extract, 5.0g peptone, 5.5 g NaCl2.5g agar and 9.0g agar into 500ml sterilized water, and adjusting pH to 7.4-7.6; the PDA slant culture medium is prepared by adding 100g of potato, 10g of glucose and 9g of agar powder into per 500ml of sterilized water, and naturally adjusting pH; the proportion of the Gao's first slant culture medium is that every 500ml of sterilized water is added with KNO30.50g, soluble starch 10.0g, K2HPO40.25g, MgSO4.7H2O 0.25g, NaCl0.25g, FeSO40.005g and agar 9g, and the pH value is adjusted to 7.4-7.6.
(3) The culture conditions are as follows: bacteria for 1-2 days, fungi for 2-3 days, and actinomycetes for 5-7 days in a biochemical incubator at 28 deg.C. When the complete bacterial colony is cultured in a biochemical incubator (bacteria 1-2 days, fungi 3 days, actinomycetes 5 days)
(4) Counting: the plate counting calculation method is carried out after the growth of the strain is stabilized to be mature strain as follows
In the formula: a-number of colonies read on each plate, one
Dilution multiple-103、104、105Etc. depending on the operation
TABLE 3 Effect of different microbial matrix improvers on soil microflora
As can be seen from table 3, the microbial environment of the primary soil changed in a series after the addition of different microbial matrix improvers: firstly, the abundances of bacteria and actinomycetes in soil are changed, the abundances of bacteria and actinomycetes communities in obstacle soil are obviously reduced along with the increase of a culture period, and the abundances of bacteria and actinomycetes in soil treated by the microbial matrix modifier are maintained or increased and are obviously higher than the abundances of the obstacle soil; secondly, the change of the fungal abundance in the primary soil is realized, the fungal abundance of the primary obstacle soil is obviously higher than that of the rest microbial matrixes, and part of strains have the effect of increasing the fungal abundance of the soil, which is contrary to the improvement aim, so that the Pf, the TR and the like have the effect of obviously reducing the fungal abundance and meet the improvement aim.
Example 5 direct soil mixing for seedling raising and improvement of seed germination and germination rate
(1) Placing seeds of Jinyou No. 4 cucumber in a 150ml conical flask, soaking the seeds in normal temperature water for 4h, removing waste liquid, taking clean clear water again in the flask, attaching a sealing film, and carrying out seeding after oscillating and accelerating germination for 20-22h at 28 ℃ and 190-one times at 210 r/min.
(2) After obtaining a mature bacterial solution in the same manner as in example 1, the solution was mixed with the matrix in the same ratio in the soil for obstacles, and the mixture was distributed into 15-hole plugs, for 60 plugs per 5 plugs.
(3) Further, punching soil, seeding 1 cucumber seed in each hole, watering sufficient root fixing water after covering soil, performing seedling management in a normal greenhouse, and calculating the germination rate when most seedlings grow to 1 leaf and 1 heart or 2 leaves and 1 heart.
The seed germination rate (%) - (number of germinated seeds/number of test seeds) × 100%
TABLE 4 influence of different microbial matrix improvers on cucumber germination
As can be seen from table 4 above comparing the microbial matrix improvers, TR, Pf, and Sg all significantly improve the germination rate of cucumber seeds, and have a very significant promoting effect on CK (soil in obstacle), among which Sg (streptomyces griseus) has the best effect.
Claims (10)
1. A microbial soil conditioner is characterized in that the microbial soil conditioner is obtained by adding functional strain fermentation liquor into a matrix according to the proportion of 10-15% (V/W), and then mixing the matrix mixed with the fermentation liquor and soil; the functional strains are selected from Trichoderma harzianum with a strain number of BNCC 336568, Pseudomonas fluorescens with a strain number of BNCC 231887 and one or more of Streptomyces griseus with a strain number of BNCC 335914, the matrix is a mixture of vinegar residue and rice straw charcoal, and the volume ratio of the vinegar residue to the rice straw charcoal is 1: 0.8-1.2; the volume ratio of the matrix to the soil is 1: 23-28.
2. The microbial soil conditioner of claim 1, wherein the concentration of said fermentation broth of said functional strain is 1 x 10 or more7CFU/ml。
3. The microbial soil conditioner according to claim 1, wherein said fermentation broth of said functional strain is added to the substrate in an amount of 10% (V/W).
4. The microbial soil conditioner of claim 1, wherein said functional strain is selected from the group consisting of Streptomyces griseus having a bacterial species number BNCC 335914.
5. The microbial soil conditioner of claim 1, wherein the mixing volume ratio of the substrate to the soil is vinegar residue: rice straw charcoal: the soil is 1: 1: 50.
6. a method for producing a microbial soil improvement agent according to any one of claims 1 to 5, characterized by comprising the steps of:
(1) liquid culturing the functional bacteria to obtain mature bacteria suspension;
(2) adjusting the concentration of the bacterial suspension by a method of counting by a blood counting plate, inoculating the bacterial suspension into the matrix, and mixing the matrix and the obstacle soil after uniform mixing.
7. Use of the microbial soil conditioner of any one of claims 1 to 5 for reducing the salinity of salinized soil and improving soil acidification.
8. Use of a microbial soil amendment as claimed in any of claims 1 to 5 for accumulating soil available nutrients.
9. Use of a microbial soil amendment of any of claims 1 to 5 for effectively regulating a soil microbial environment.
10. Use of the microbial soil conditioner of any one of claims 1 to 5 for reducing salinity content of salinized soil, improving soil acidification, accumulating soil available nutrients and/or effectively regulating soil microbial environment.
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| CN113265254A (en) * | 2021-07-01 | 2021-08-17 | 山西大地生态环境技术研究院有限公司 | Microcapsule type soil conditioner containing microorganisms and preparation method and application thereof |
| CN113735651A (en) * | 2021-08-26 | 2021-12-03 | 黑龙江谷馨源肥业有限公司 | Soil remediation improver and preparation method thereof |
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