CN112521948B

CN112521948B - Fertilizing method for improving secondary salinization greenhouse soil

Info

Publication number: CN112521948B
Application number: CN202011385299.9A
Authority: CN
Inventors: 李世贵; 顾金刚; 任萍
Original assignee: Institute of Agricultural Resources and Regional Planning of CAAS
Current assignee: Institute of Agricultural Resources and Regional Planning of CAAS
Priority date: 2020-12-01
Filing date: 2020-12-01
Publication date: 2022-04-15
Anticipated expiration: 2040-12-01
Also published as: CN112521948A

Abstract

The invention discloses a fertilizing method for improving secondary salinization greenhouse soil. The fertilizing method comprises the step of applying an organic fertilizer and a microbial inoculum to the secondary salinization greenhouse soil to be improved to improve the secondary salinization greenhouse soil to be improved, wherein the active ingredient of the microbial inoculum is paenibacillus. After the organic fertilizer is applied as a base fertilizer, the paenibacillus milrinus microbial inoculum is applied to improve the secondary salinized soil into neutral soil, so that the EC value and the organic matter content of the secondary salinized soil are improved, and the cucumber yield is increased. The invention can be used for improving secondary salinization soil and increasing cucumber yield.

Description

Fertilizing method for improving secondary salinization greenhouse soil

Technical Field

The invention relates to a fertilizing method for improving secondary salinization greenhouse soil.

Background

Secondary salinization (also called "soil secondary salinization") is a process of salinization of cultivated soil due to unreasonable artificial measures.

The formation of secondary salinization of soil and the salinization degree are closely related to facility environment and artificial farming management. The facility cultivation is a long-term closed cultivation mode, so that the temperature in the facility is higher than the outside all the year round, the full leaching of natural rainwater and the freezing and thawing of soil in winter and spring cannot be obtained, and in addition, the frequent watering is carried out to adjust the ground temperature and the air temperature of the over-summer cultivation, so that the transpiration and the evaporation of the soil moisture are always stronger than that of the open ground. According to the rule that salt moves with water, salt is bound to gather towards the surface layer. Unlike open field cultivation, perennial or seasonal covering changes the water moving direction of soil naturally, and the water moves upwards through capillary action because of high temperature and strong evaporation in the greenhouse and the water moves from underground to earth surface, so that salt is accumulated in soil surface. In addition, the salt is not leached by rainwater in the facility for a long time, and the salt cannot be leached by the rainwater like the open field, so that the salt is further accumulated in a large amount. The blind mass fertilization is a direct cause of the secondary salinization of facility soil. Under the condition of facility cultivation, the vegetables have the advantages of high growth speed, high yield, multiple stubbles and good benefit, and farmers often apply a large amount of chemical fertilizers and organic fertilizers to each stubble.

In recent years, the problem of salinization of soil in protection fields such as plastic greenhouses, solar greenhouses and the like is increasingly serious, and crop yield reduction and agricultural product quality reduction are directly caused.

And (4) the pH value of the soil to measure the strength of the acid-base reaction of the soil. Mainly determined by the concentration of hydrogen and hydroxide ions in the soil solution, expressed as pH. Neutral soil with pH of 6.5-7.5; acid soil below 6.5; alkaline soil is above 7.5. The pH value of the soil is a key index for characterizing the quality of the secondary salinized soil.

Disclosure of Invention

The invention aims to solve the technical problem of how to reduce the pH value of secondary saline soil, saline-alkali soil or alkaline soil and/or improve the saline-alkali soil or alkaline soil.

In order to solve the above problems, the present invention provides the following technical solutions:

1. use of a paenibacillus or a composition for lowering the pH of soil; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the soil is secondary saline soil, saline-alkali soil or alkaline soil.

2. Use of a paenibacillus or a composition for the preparation of a product for reducing the pH of soil; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the soil is secondary saline soil, saline-alkali soil or alkaline soil.

3. The use of a paenibacillus or composition for improving soil; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the soil is secondary saline soil, saline-alkali soil or alkaline soil.

4. Use of a paenibacillus or a composition in the preparation of a product for improving soil; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the soil is secondary saline soil, saline-alkali soil or alkaline soil.

5. The application of the paenibacillus or the composition in improving soil of a protected area; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the protective land soil is secondary salinization protective land soil, alkaline protective land soil or saline-alkaline protective land soil.

6. The application of the Paenibacillus or the composition in preparing a product for improving soil of a protected area; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the protective land soil is secondary salinization protective land soil, alkaline protective land soil or saline-alkaline protective land soil.

7. Application of Paenibacillus in increasing cucumber yield.

8. Application of Paenibacillus in preparing products for improving cucumber yield.

9. The fertilizing method for improving the secondary salinization greenhouse soil comprises the step of applying an organic fertilizer and the microbial inoculum to the secondary salinization greenhouse soil to be improved, so that the secondary salinization greenhouse soil to be improved is improved.

As used herein above, the Paenibacillus may be Paenibacillus griseus (Paenibacillus jamilae) ACCC 03134.

In the above, the product may be a microbial inoculum or a fertilizer containing the microbial inoculum.

The pH of the soil in 1 to 4 above is 8 or more, for example, 8.1 to 8.6; the lowering the pH of the soil may be lowering the pH of the soil to 7.0-7.1.

The EC value of the soil in the above 1 to 4 is less than 0.4 ms/cm.

In the above 3 and 4, the improved soil may be at least one of:

A1) reducing the pH value of the soil to 7.0-7.1,

A2) increasing the EC value of the soil to 0.4ms/cm to 1.0ms/cm, such as 0.7 to 0.8ms/cm,

A3) increasing the organic matter content of the soil,

A4) increasing the total nitrogen content and/or the alkaline hydrolysis nitrogen content of the soil,

A5) the total phosphorus content of the soil is improved,

A6) increasing Na content of said soil⁺The content of the components is as follows,

A7) increasing Cl of said soil^-The content of the components is as follows,

A8) increasing the available potassium content of the soil.

The pH of the soil for protection in the above items 5 and 6 is 8 or more, for example, 8.1 to 8.6.

The EC value of the soil for the protected area in the above 5 and 6 is less than 0.4 ms/cm.

In the above 5 and 6, the soil for improving and protecting the soil may be at least one of:

B1) reducing the pH value of the soil of the protected area to 7.0-7.1,

B2) increasing the EC value of the soil in the protected area to 0.4ms/cm-1.0ms/cm, such as 0.7-0.8ms/cm,

B3) the organic matter content of the soil of the protected land is improved,

B4) increasing the total nitrogen content and/or the alkaline hydrolysis nitrogen content of the soil of the protected area,

B5) the total phosphorus content of the soil of the protected area is improved,

B6) increasing Na content in the soil of the protected area⁺The content of the components is as follows,

B7) increasing Cl of the soil of the protected area^-The content of the components is as follows,

B8) increasing the quick-acting potassium content of the soil of the protected area.

The microbial inoculum or the composition also belongs to the protection scope of the invention.

The composition may have at least one of the properties 1) to 3) above. In the composition, the cucumber is a propagation material, such as seeds. The microbial inoculum and the cucumber can be packaged independently.

The microbial inoculum may also comprise a carrier, as hereinbefore described. The carrier may be a solid carrier or a liquid carrier. The solid carrier is a mineral material, a plant material or a high molecular compound; the mineral material is at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica and diatomite; the plant material is at least one of corn flour, bean flour and starch; the high molecular compound is polyvinyl alcohol and/or polyglycol. The liquid carrier is organic solvent, vegetable oil, mineral oil or water; the organic solvent is decane and/or dodecane. In the microbial inoculum, the active ingredient may be present in the form of cultured living cells, a fermentation broth of living cells, a filtrate of a cell culture, or a mixture of cells and a filtrate. The living cells may be in the form of conidia, chlamydospores, hyphae or mycelium containing conidia and hyphae, preferably conidia or chlamydospores. The composition can be prepared into various dosage forms, such as liquid, emulsion, suspending agent, powder, granules, wettable powder or water dispersible granules.

According to the requirement, the microbial inoculum can also be added with a surfactant (such as Tween 20, Tween 80 and the like), a binder, a stabilizer (such as an antioxidant), a pH regulator and the like.

Experiments prove that the Paenibacillus griseus ACCC03134 can be used for treating alkaline soil (pH)>7.5) improving the soil to be neutral, improving the EC value of high-pH value and low-EC value soil, and improving the organic matter content, total nitrogen content, total phosphorus content and Na content of the soil⁺Content, Cl^-Content and/or rapid-acting potassium content. After the organic fertilizer is applied as a base fertilizer, the paenibacillus milrinus microbial inoculum is applied to improve the secondary salinized soil into neutral soil, so that the EC value and the organic matter content of the secondary salinized soil are improved, and the cucumber yield is increased. Book (I)The invention can be used for improving secondary salinization soil and increasing cucumber yield.

Detailed Description

The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.

The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

In the following examples, Paenibacillus grisea (Paenibacillus jamilae) ACCC03134 was collected in the Agricultural microorganism center of China Committee for Culture Collection of microorganisms (ACCC) before the filing date of the present application, also known as the China Agricultural Culture Collection of China Agricultural microorganisms (ACCC, address: No. 12 south China Union of Guancun, Hakko, Beijing), the institute of Agricultural resources and Agricultural regionalization, Japan 100081). The collection date of Paenibacillus grisea (Paenibacillus jamilae) ACCC03134 is 12.20.2007, and the strain can be obtained by the public from the agricultural microorganism center of the China Committee for culture Collection of microorganisms from the date of collection. The ACCC is provided with a special website, and the website address is as follows: http:// www.accc.org.cn, the public can directly order strains on the internet. The website of Paenibacillus grisea ACCC03134 is http:// www.accc.org.cn/Column _ content. aspColumn _ ID. 48571& pid. 10203756.

Example 1 improvement of Secondary salinization greenhouse soil (alkaline greenhouse soil)

1. Preparation of Paenibacillus milbefaciens agent

The preparation method of the medium used in this example was as follows:

beef extract culture medium: 20g of glucose, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract and 20g of agar, wherein the volume is determined to be 1L by distilled water, the pH value is 7.0, and the sterilization is carried out for 15min at 121 ℃.

Fermentation medium of Paenibacillus: 15g of cane sugar, 10g of bean pulp, (NH)₄)₂SO₄ 4g、K₂HPO₄ 0.5g,MgSO₄·7H₂O0.1 g, distilled water to a constant volume of 1L, pH6.5, and sterilizing at 121 deg.C for 15 min.

Inoculating Paenibacillus milciaus ACCC03134 (Paenibacillus milae) in a beef extract culture plate, culturing for 72h at 37 ℃, scraping off spores on one culture plate, inoculating the spores in a 500mL triangular flask filled with 200mL fermentation medium of the Paenibacillus, and culturing for 72h at 37 ℃ and 220 rpm; then inoculating the strain into a 15L seed tank containing 8L of fermentation medium of Paenibacillus according to the inoculation amount of 2% (v/v), culturing for 24h under the conditions of 37 ℃, 220rpm, pH7.0 and ventilation rate of 0.8vvm, then inoculating the strain into a 100L fermentation tank containing 70L of fermentation medium of Paenibacillus according to the inoculation amount of 5% (v/v), and culturing for 4d under the conditions of 37 ℃, 220rpm, pH7.0 and ventilation rate of 1.0vvm to obtain the Paenibacillus milrinus fermentation liquor. Mixing the Paenibacillus milbefaciens fermentation liquor with sterilized diatomite to obtain the Paenibacillus milbefaciens agent, wherein the content of the Paenibacillus milbefaciens (Paenibacillus) ACCC03134 in the Paenibacillus milaeus agent is 4 multiplied by 10⁸cfu/g。

2. Improving the soil of the secondary salinization greenhouse and improving the growth condition of the cucumbers.

2.1 test site

The field plot experiment is arranged in three greenhouses of a water-viewing village of Wang Gao shop in Pingyuan county of Shandong Texiao, the test plots are respectively a greenhouse for continuously planting cucumbers for 3 years (hereinafter referred to as a continuous cropping 3-year greenhouse), a greenhouse for continuously planting cucumbers for 8 years (hereinafter referred to as a continuous cropping 8-year greenhouse) and a greenhouse for continuously planting cucumbers for 11 years (hereinafter referred to as a continuous cropping 11-year greenhouse), the soil of the 3 greenhouses is moist soil, and the 3 test plots are planted with cucumbers in one season.

Adopting a five-point sampling method in each greenhouse, taking 100g of soil (hereinafter referred to as initial soil) near the rhizosphere of the cucumber at each point, bringing the soil back to a laboratory, and detecting the pH according to the water-soil ratio of 5: 1; and performing EC (electric conductivity method, HJ802-,Organic content (volumetric method, NY/T1121.6-2006), total nitrogen content (volumetric method, LY/T1228-2015), alkaline-hydrolyzed nitrogen content (volumetric method, LY/T1228-2015), total phosphorus content (UV-visible spectrophotometry, LY/T1232-2015), Na⁺Content (flame photometry, LY/T1251-^-Content (volumetric method, NY/T1121.17-2006) and quick-acting potassium content (inductively coupled plasma atomic emission spectrometry, LY/T1234-2015). The results are shown in Table 1.

TABLE 1 physicochemical Properties of respective greenhouse soils (i.e., initial soils)

In table 1, the greenhouse ages of 11 years, 8 years and 3 years are greenhouse initial soil for 3 years of continuous cropping, greenhouse initial soil for 8 years of continuous cropping and greenhouse initial soil for 11 years of continuous cropping, respectively.

The results show that: the soil of the three greenhouses belongs to secondary salinization soil, the secondary salinization degree of the greenhouse soil is the highest in 8-year continuous cropping, and the secondary salinization degree of the greenhouse soil is the lowest in 11-year continuous cropping.

2.2 field test implementation plan

2.2.1 Experimental cell settings

The greenhouse is continuously planted for 3 years at 0.133 hectare, 60 furrows are formed in the greenhouse by equally dividing the greenhouse into equal areas at equal intervals, 2 furrows are formed in each furrow, 60 cucumbers are planted in each furrow, protective areas are arranged on two sides of the greenhouse, 10 furrows are respectively arranged in each protective area, three test areas are arranged in the middle of the greenhouse, each test area comprises 8 cells, and each cell comprises 5 furrows. The three test areas are respectively a control treatment area (CK), a compound organic fertilizer treatment area (R) and a microbial inoculum M treatment area (M). The contrast treatment area (CK) is provided with 4 cells, the compound organic fertilizer treatment area (R) is provided with 3 cells, and the microbial inoculum M treatment area (M) is provided with 3 cells.

The greenhouse is continuously planted for 8 years for 0.087 hectare, the greenhouse is divided into 62 ridges with equal area at equal intervals, 2 furrows are formed in each ridge, 36 cucumbers are planted in each furrow, protective areas are arranged on two sides of the greenhouse, the protective areas are 7 ridges respectively, three test areas are arranged in the middle, each test area comprises 8 cells, and each cell comprises 6 ridges. The three test areas are respectively a control treatment area (CK), a compound organic fertilizer treatment area (R) and a microbial inoculum M treatment area (M). The contrast treatment area (CK) is provided with 4 cells, the compound organic fertilizer treatment area (R) is provided with 3 cells, and the microbial inoculum M treatment area (M) is provided with 3 cells.

The greenhouse is continuously planted for 11 years, the greenhouse is 0.1 hectare in total, 86 ridges are equally divided in area at equal intervals, 2 furrows are formed in each ridge, 34 cucumbers are planted in each furrow, protective areas are arranged on two sides of the greenhouse, 11 ridges are respectively arranged in each protective area, three test areas are arranged in the middle, each test area comprises 8 cells, and each cell comprises 8 ridges. The three test areas are respectively a control treatment area (CK), a compound organic fertilizer treatment area (R) and a microbial inoculum M treatment area (M). The contrast treatment area (CK) is provided with 4 cells, the compound organic fertilizer treatment area (R) is provided with 3 cells, and the microbial inoculum M treatment area (M) is provided with 3 cells.

In order to facilitate the statistics of the yield, the test area and the test cell in each greenhouse are divided into equal parts, the number of the cultivation ridges of the test cell is consistent, and the number of seedlings is kept consistent as much as possible. After the selected greenhouse is tested, the experimental area and the setting area of the cell of each greenhouse need to be fixed and cannot be changed randomly, and the nameplates are inserted into the starting point and the ending point of each experimental cell so as to record data conveniently.

2.2.2 cell test

And (3) carrying out high-temperature greenhouse closing sterilization on the greenhouse from 7 to 9 months in 2018, the greenhouse from 8 years in continuous cropping and the greenhouse from 11 years in continuous cropping, and preventing plant diseases and insect pests in the greenhouse. In last ten days of 9 months, rice hull and chicken manure are applied to the initial soil of the continuous cropping greenhouse of 3 years, the initial soil of the continuous cropping greenhouse of 8 years and the initial soil of the continuous cropping greenhouse of 11 years according to the traditional fertilization mode of farmers, wherein the weight of the rice hull and the chicken manure is 1200kg per mu; preparing soil after half a month in the slow field, dividing test cells, fertilizing according to different test treatments and uniformly mixing the fertilizer with soil.

Applying sterilized diatomaceous earth 20kg per acre in a control treatment zone (CK); applying a compound organic fertilizer (model: general type S1, Dezhou city Yuan and agriculture science and technology development, LLC) in the compound organic fertilizer treatment area (R) according to 200kg per mu; after 200kg of compound organic fertilizer (type: general type S1, Dezhou city Yuan and agricultural science and technology development Limited liability company) is applied to each mu of the microbial inoculum M treatment area, 20kg of the Bacillus milrinus microbial inoculum obtained in the step (1) and the application amount of the Bacillus milrinus microbial inoculum are applied to each mu of the microbial inoculum M treatment areaThe input quantity is 10 per square meter¹⁰cfu plus Paenibacillus milaeus (Paenibacillus) ACCC 03134. All test districts do not use chemical agents to prevent and control diseases.

2.2.3 cultivation management of cucumber

Cucumber seedlings tested in the field plot are purchased locally in Dezhou, Shandong, and the cucumber variety is Jinyou 316.

Transplanting the cucumber seedlings in a three-leaf one-heart period, wherein the cucumber seedlings are all grafted seedlings. The management mode of the three test areas is based on local traditional cultivation, and the moisture and other management measures are kept consistent.

3. Data processing

All data were analyzed for significance using the IBM SPSS 22.

4 results and analysis

4.1 Effect of different fertilization treatments on cucumber yield

The cucumber is picked in different time periods due to different planting time periods, and the cucumber is picked once in 2d generally in each greenhouse. The effect of each picking time and different treatments on cucumber yield is listed in tables 2-3.

TABLE 2 melon plucking period

Age of shed	Time to first picking	Final picking time
			For 3 years	11/2018, 28/11/month	29/5/2019
8 years old	12 months and 14 days in 2018	6 months and 20 days in 2019
			11 years old	12 month and 8 days 2018	6 months and 4 days in 2019

And (4) converting the yield of each cucumber according to the yield of each cell of each greenhouse.

TABLE 3 Effect of different treatments on cucumber yield

Note: the english letters following each column number indicate the degree of significance of the difference, with no significant difference at the 0.05 level between treatments of the same letter and significant difference at the 0.05 level between treatments of different letters.

Table 3 the results show that: the effect of different treatments on cucumber yield varies. The yield of the M treatment is increased by 6.37 percent in a greenhouse with 3 years of continuous cropping, 17.15 percent in a greenhouse with 8 years of continuous cropping and 9.25 percent in a greenhouse with 11 years of continuous cropping. The yield increasing effect of M treatment in the continuous cropping greenhouse is the highest in 8 years.

The cucumber yield is recorded completely, the rhizosphere soil of the cucumbers is taken when the cucumbers pull seedlings, three points are taken in each cell in each greenhouse, then the samples of the same cells in each greenhouse are mixed together, and the samples are marked. And (3) sending each part of the marked sample to a Panni test group company Limited to detect the physicochemical property of the soil: pH, EC (conductivity method, HJ802-2016), organic content (volumetric method, NY/T1121.6-2006), total nitrogen content (volumetric method, LY/T1228-2015), alkali-hydrolyzable nitrogen content (volumetric method, LY/T1228-2015), total phosphorus content (ultraviolet-visible spectrophotometry, LY/T1232-2015), Na⁺Content (flame photometry, LY/T1251-^-Content (volumetric method, NY/T1121.17-2006) and quick-acting potassium content (inductively coupled plasma atomic emission spectrometry, LY/T1234-2015).

TABLE 4 physicochemical properties of soil treated by different fertilizers in 3-year continuous cropping greenhouse

As can be seen from Table 4: the original soil has pH of 8.38 and EC value of 36.0mS/m, and belongs to high pH value and low EC value type soil (pH value)>7.5,EC<0.4 (ms/cm). Compared with the original soil of the last year, the cucumber greenhouse soil for 3 years of continuous cropping has the soil pH value reduced in CK, R and M treatment areas, and the soil pH value is reduced most in the M treatment area; EC value, organic matter content, total nitrogen content, total phosphorus content and Na of soil⁺Content, Cl^-The content and the quick-acting potassium content increase most under the M treatment condition. The soil in the CK treatment area is alkaline soil (the pH value is 7.90), the soil in the R treatment area and the soil in the M treatment area are neutral soil (the pH values are 7.50 and 7.10 respectively), and the alkaline soil (the pH value is 7.50) can be treated by adding Paenibacillus milae ACCC03134>7.5) improving the soil to be neutral soil, and increasing the EC value of the soil with low EC value to a proper interval; can improve the organic matter content, total nitrogen content, total phosphorus content and Na content of soil⁺Content, Cl^-Content and quick-acting potassium content.

TABLE 5 physicochemical properties of soil treated by different fertilizers in 8-year continuous cropping greenhouse

As can be seen from Table 5: the original soil has pH of 8.45 and EC value of 21.52mS/m, and belongs to high-pH value and low-EC value type soil (pH value)>7.5,EC<0.4 (ms/cm). Compared with the original soil of the last year, the soil in the cucumber greenhouse for continuous cropping of 8 years has the advantages that the pH value of the soil is reduced in CK, R and M treatment areas, and is reduced most in the M treatment area; EC value, organic matter content, alkaline hydrolysis nitrogen content and Na of soil⁺Content, Cl^-The content and the quick-acting potassium content increase most under the M treatment condition. The soil in the CK treatment zone was alkaline (pH 8.00), and the soil in the R and M treatment zones was neutral (pH 7.40 and 7.00, respectively), indicating that Pae, is Paenibacillus milrinusNibacillus jamilae) ACCC03134 can remove alkaline soil (pH)>7.5) improving the soil to be neutral soil, and increasing the EC value of the soil with low EC value to a proper interval; can improve the content of organic matters, the content of alkaline hydrolysis nitrogen and Na in soil⁺Content, Cl^-Content and quick-acting potassium content.

TABLE 6 physicochemical properties of different fertilization treated soils in greenhouses for 11 years of continuous cropping

As can be seen from Table 6: the original soil has pH of 8.26 and EC value of 23.80mS/m, and belongs to high pH value and low EC value type soil (pH value)>7.5,EC<0.4 (ms/cm). Compared with the original soil of the last year, the cucumber greenhouse soil for 11 years of continuous cropping has the advantages that the pH value of the soil is reduced in CK, R and M treatment areas, and is reduced most in the M treatment area; soil EC value, organic matter content, total phosphorus content and Cl^-The content increases most under the M treatment conditions. The soil in the CK treatment area is alkaline soil, the soil in the R treatment area and the soil in the M treatment area are neutral soil, and the result shows that the Bacillus Calmette-Gurley (Paenibacillus jamilae) ACCC03134 can be used for treating the alkaline soil (pH)>7.5) improving the soil to be neutral soil, and increasing the EC value of the soil with low EC value to a proper interval; can improve the content of organic matters and Cl in soil^-And (4) content.

Experiments prove that after the compound organic fertilizer is applied as a base fertilizer, the secondary salinized soil can be improved into neutral soil by applying the Paenibacillus milbefaciens agent, the EC value and the organic matter content of the secondary salinized soil are improved, and the cucumber yield is increased. The invention can be used for improving secondary salinization soil and increasing cucumber yield.

The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.

Claims

1. The application of the paenibacillus or the composition in improving soil of a protected area; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the protective land soil is alkaline protective land soil, saline-alkali protective land soil or secondary salinization protective land soil;

the paenibacillus is Paenibacillus Calmii (B.manshuriensis) (B.manshuriensis)Paenibacillus jamilae）ACCC 03134；

The EC value of the soil for the protected land is less than 0.4ms/cm, and the soil for the improved protected land is B2), B3) and B7) as follows:

B2) the EC value of the soil of the protected area is increased to 0.7-0.8ms/cm,

B3) the organic matter content of the soil of the protected land is improved,

B7) increasing Cl of the soil of the protected area^-And (4) content.

2. The application of the Paenibacillus or the composition in preparing a product for improving soil of a protected area; the composition is a composition A or a composition B, wherein the composition A consists of cucumbers and a microbial inoculum, and the composition B consists of cucumbers, an organic fertilizer and the microbial inoculum; the active ingredient of the microbial inoculum is the paenibacillus; the protective land soil is alkaline protective land soil, saline-alkali protective land soil or secondary salinization protective land soil;

B3) the organic matter content of the soil of the protected land is improved,

B7) increasing Cl of the soil of the protected area^-And (4) content.