CN110218679A - A kind of secondary salinization soil remediation microbial inoculum and its application - Google Patents
A kind of secondary salinization soil remediation microbial inoculum and its application Download PDFInfo
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Abstract
The present invention provides a kind of secondary salinization soil remediation microbial inoculum and its applications, belong to technical field of land improvement, and the microbial inoculum includes the raw material of following volumes part: 0.5~1.5 part of 0.5~1.5 part of bacillus licheniformis agent and gel-shaped series bacillus microbial inoculum；The living bacteria count of bacillus licheniformis is 30~4,000,000,000 cfu/mL in the bacillus licheniformis agent；The concentration of gel-shaped series bacillus is 15~2,500,000,000 cfu/mL in the gel-shaped series bacillus microbial inoculum；The deposit number of the bacillus licheniformis is CGMCC No.10741；The deposit number of the gel-shaped series bacillus is CGMCC No.3995.Secondary salinization soil is repaired using microbial inoculum of the invention, repairing effect is significant, can not only reduce content of soil nitrate-N, increase soil fertility, while also can be improved crop yield and edible safety.
Description
Technical field
The present invention relates to technical field of land improvement more particularly to a kind of secondary salinization soil remediation microbial inoculum and its answer
With.
Background technique
Industrialized agriculture is the important production method of modern agriculture.China is an industrialized agriculture big country, wherein facilities vegetable
It is largest.Facility cultivation agricultural has played huge effect in China vegetables and other Important Economic arable farmings, obtains
Significant economy and society effect.For example, nowadays we " winter eats summer vegetable, the summer eats preserved vegetable, in eat western food, southern dish is eaten in north " need
It asks and is satisfied, be mainly still attributed to the fact that the development of China's industrialized agriculture.But as excessive application chemical fertilizer and greenhouse are high
Temperature, high humility lack the factors such as rainwater elution, and soil physico-chemical property is made to change, and gradually form height and cure, lead to soil
Lead to the problem of many productions such as unbalanced secondary salinization, nutrient, soil hardening, soil acidification.Wherein soil secondary salinization
The most prominent, main feature shows as total soil phosphorus height, salt accumulation in surface soil.
The approach that tradition solves soil secondary salinization has: washing salinity by irrigation method, soil conditioner method, organic fertilizer method semi-decomposed
And plantation salt-tolerant plant kind etc., these methods easily cause secondary pollution, can not fundamentally solve the secondary salt of soil
Stain problem.
Summary of the invention
The purpose of the present invention is to provide a kind of secondary salinization soil remediation microbial inoculum and its application, which repairs secondary
Salinization soil significant effect, and not will cause secondary pollution.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of secondary salinization soil remediation microbial inoculum, the raw material including following volumes part: lichens gemma
0.5~1.5 part of 0.5~1.5 part of bacillus microbial inoculum and gel-shaped series bacillus microbial inoculum；
The living bacteria count of bacillus licheniformis is 30~4,000,000,000 cfu/mL in the bacillus licheniformis agent；
The concentration of gel-shaped series bacillus is 15~2,500,000,000 cfu/mL in the gel-shaped series bacillus microbial inoculum；
The deposit number of the bacillus licheniformis is CGMCC No.10741；The preservation of the gel-shaped series bacillus
Number is CGMCC No.3995.
Preferably, the microbial inoculum further includes organic carrier；The organic carrier includes agricultural wastes.
Preferably, the organic carrier includes one or more of mushroom slag, bean cake powder and stalk.
Preferably, the partial size of the organic carrier is 60~100 mesh.
Preferably, the bacillus licheniformis agent and gel-shaped series bacillus microbial inoculum total volume and the organic carrier
The ratio of gross mass is 1mL:8~10g.
The present invention also provides secondary salinization soil remediation microbial inoculums described in above scheme in secondary salinization soil reparation
In application, comprising the following steps: the secondary salinization soil remediation microbial inoculum is applied to secondary salinization soil to be repaired.
Preferably, the mode of the application includes that cave is applied and/or spread fertilizer over the fields.
Preferably, when the mode of the application is applied for cave, the amount of application of the secondary salinization soil remediation microbial inoculum is
The cave 80~120g/.
Preferably, when the mode of the application is to spread fertilizer over the fields, the amount of application of the secondary salinization soil remediation microbial inoculum is
20~150kg/666.7m2。
Beneficial effects of the present invention: the present invention provides a kind of secondary salinization soil remediation microbial inoculums, including following volumes
The raw material of part: 0.5~1.5 part of 0.5~1.5 part of bacillus licheniformis agent and gel-shaped series bacillus microbial inoculum；The lichens
The living bacteria count of bacillus licheniformis is 30~4,000,000,000 cfu/mL in gemma bacillus agent；The gel-shaped series bacillus bacterium
The concentration of gel-shaped series bacillus is 15~2,500,000,000 cfu/mL in agent；The deposit number of the bacillus licheniformis is CGMCC
No.10741；The deposit number of the gel-shaped series bacillus is CGMCC No.3995.Utilize secondary salinization of the invention
Soil remediation microbial inoculum repairs secondary salinization soil, and repairing effect is significant, can not only reduce content of soil nitrate-N, improve soil
Earth fertility, while also can be improved crop yield and edible safety.
Detailed description of the invention
Fig. 1 is strain enzyme-producing vigor situation in embodiment 3；
Fig. 2 is that bacterial strain improves the hardened situation of secondary salinization soil in embodiment 4.
Specific embodiment
The present invention provides a kind of secondary salinization soil remediation microbial inoculum, the raw material including following volumes part: lichens gemma
0.5~1.5 part of 0.5~1.5 part of bacillus microbial inoculum and gel-shaped series bacillus microbial inoculum；Preferably, the secondary salinization soil
Remediation microbial inoculum includes the raw material of following volumes part: 1 part of 1 part of bacillus licheniformis agent and gel-shaped series bacillus microbial inoculum；Institute
The living bacteria count for stating bacillus licheniformis in bacillus licheniformis agent is 30~4,000,000,000 cfu/mL, preferably 3,500,000,000 cfu/
mL；In the gel-shaped series bacillus microbial inoculum concentration of gel-shaped series bacillus be 15~2,500,000,000 cfu/mL, preferably 20
Hundred million cfu/mL；The bacillus licheniformis is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center
(CGMCC), deposit number is CGMCC No.10741, and the deposit date is on Mays 22nd, 2015；The gel-shaped series bacillus
Purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number CGMCC
No.3995, the deposit date is on October 27th, 2010.In the present invention, the bacillus licheniformis and gel-shaped series bacillus
All be conducive to the formation of the hardened soil agreegate of salination, and the combination of two bacterium is better than single bacterial strain function and effect.
In the present invention, the microbial inoculum preferably further includes organic carrier；The organic carrier includes agricultural wastes；It is described
Agricultural wastes preferably include one or more of mushroom slag, bean cake powder and stalk；The mushroom slag is preferably Pleurotus eryngii mushroom slag；
The Pleurotus eryngii mushroom slag is preferably purchased from the planting edible mushroom family on factory periphery.
The partial size of organic carrier of the present invention is preferably 60~100 mesh, more preferably 80 mesh；The organic carrier is preferred
Prepared using following methods: agricultural wastes are crushed, are sterilized, organic carrier is obtained；The equipment of the crushing
Preferably chain crusher；The temperature of the sterilizing is preferably 115~121 DEG C；The time of the sterilizing is preferably 25~
35min, more preferably 30min；The equipment of the sterilizing is preferably the rotatable culture medium sterilizer of stainless steel.In the present invention,
The effect of the organic carrier is: 1. adsorption function bacterium；2. providing nutrition for strain；3. improving soil with organic matter, improve soil
Earth structure.
Secondary salinization soil remediation microbial inoculum of the present invention is preferably prepared using following methods, including following step
It is rapid:
1) bacillus licheniformis is inoculated in the first fermentation medium, fermented, obtain Bacillus licheniformis
Agent；
2) the gel-shaped series bacillus is inoculated in the second fermentation medium, fermented, obtain gel-shaped class bud
Spore bacillus microbial inoculum；
3) the step 1) bacillus licheniformis agent and step 2) the gel-shaped series bacillus microbial inoculum are mixed
It closes, obtains secondary salinization soil remediation microbial inoculum；
There is no time sequencing limitation between the step 1) and step 2)；
First fermentation medium includes following raw material: in terms of 1L, 13~17g of sucrose, 8~12g of soy meal, di(2-ethylhexyl)phosphate
The water of 0.5~1.5g of hydrogen potassium, 0.05~0.15g of zinc sulfate and surplus；
Second fermentation medium includes following raw material: in terms of 1L, 5~6g of starch, 1.5~1.6g of yeast powder, dregs of beans
5~6g of powder, 1.2~1.6g of magnesium sulfate, 1.8~2.2g of dipotassium hydrogen phosphate, 8~9g of calcium carbonate, 0.1~0.3g of sodium chloride and surplus
Water.
It preferably further include difference before fermenting to bacillus licheniformis and gel-shaped series bacillus in the present invention
Bacillus licheniformis and gel-shaped series bacillus are activated.
Bacillus licheniformis is inoculated in activation medium by the present invention, is activated, the lichens gemma activated
Bacillus；The activation medium preferably includes following raw material: in terms of 1L, 8~12g of peptone, beef extract 2~4g, NaCl3~
7g, the water of 16~20g of agar and surplus；It is furthermore preferred that the activation medium includes following raw material: in terms of 1L, peptone
10g, beef extract 3g, NaCl5g, the water of agar 18g and surplus；The pH of the activation medium is preferably 7.0~7.4, more preferably
It is 7.2；The present invention is not particularly limited the inoculum concentration of the bacillus licheniformis, using this field traditional vaccination amount；
The temperature of the activation is preferably 28~30 DEG C, and more preferably 29 DEG C；The time of the activation is preferably 24~28h, more preferably
For 26h.
Gel-shaped series bacillus is inoculated in activation medium by the present invention, is activated, the jelly activated
Sample series bacillus；The activation medium preferably includes following raw material: in terms of 1L, 8~12g of sucrose, dipotassium hydrogen phosphate 0.4
~0.6g, 0.1~0.3g of magnesium sulfate, 0.1~0.3g of sodium chloride, 0.5~1.5g of calcium carbonate, 15~25g of agar powder and surplus
Water；It is furthermore preferred that the activation medium includes following raw material: in terms of 1L, sucrose 10g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate
The water of 0.2g, sodium chloride 0.2g, calcium carbonate 1g, agar powder 20g and surplus；The present invention connects the gel-shaped series bacillus
Kind amount is not particularly limited, using this field traditional vaccination amount；The temperature of the activation is preferably 28~30 DEG C, more preferably
It is 29 DEG C；The time of the activation is preferably 24~28h, more preferably 26h.
After the bacillus licheniformis activated, the bacillus licheniformis of activation is inoculated in the first fermented and cultured by the present invention
Base ferments, and obtains bacillus licheniformis agent；Preferably, first fermentation medium includes following raw material: with 1L
Meter, the water of sucrose 15g, soy meal 10g, potassium dihydrogen phosphate 1g, zinc sulfate 0.1g and surplus；The pH of first fermentation medium
Preferably 6.5~7.0, more preferably 6.8；The temperature of the fermentation is preferably 28~30 DEG C, and more preferably 29 DEG C；The fermentation
Time be preferably 48~72h, more preferably 60h；The mode of the fermentation is preferably shake flask fermentation.
After the gel-shaped series bacillus activated, the gel-shaped series bacillus of activation is inoculated in second by the present invention
Fermentation medium ferments, and obtains gel-shaped series bacillus microbial inoculum；Preferably, second fermentation medium include with
Lower raw material: in terms of 1L, starch 5.5g, yeast powder 1.579g, bean cake powder 5.76g, magnesium sulfate 1.4g, dipotassium hydrogen phosphate 2g, carbonic acid
The water of calcium 8.5g, sodium chloride 0.2g and surplus；The pH of second fermentation medium is preferably 7.0~7.5, and more preferably 7.2
~7.4；The temperature of the fermentation is preferably 28~30 DEG C, and more preferably 29 DEG C；The time of the fermentation is preferably 48~72h,
More preferably 60h；The mode of the fermentation is preferably shake flask fermentation.
The present invention preferably further includes the jelly after fermenting after fermenting to the gel-shaped series bacillus of activation
Sample series bacillus fermentation liquid is inoculated in the second fermentation medium, expands culture；The gel-shaped series bacillus fermentation
The percent by volume that the inoculum concentration of liquid accounts for the second fermentation medium is preferably 5%；The temperature for expanding culture is preferably 30~
35 DEG C, more preferably 32~34 DEG C；The time for expanding culture is preferably 3~5d, (light splitting light until reaching strain logarithmic phase
Degree meter measurement OD600, using the culture medium that is not inoculated with as blank control, about 10~2,000,000,000 cfu/mL of bacterium number)；The expansion training
Feeding equipment is preferably fermentor.The present invention is preferred with ventilation and stirring in expanding incubation；For 24 hours into culture
Interior, ventilatory capacity is preferably 10~20m3/ h, more preferably 15m3/h；After culture for 24 hours, ventilatory capacity is preferably 15~24m3/ h, it is more excellent
It is selected as 20m3/h；The frequency of the stirring is preferably 1~2 time/h, and the time stirred every time is preferably 10~20min, more preferably
For 15min.
The present invention preferably further includes the lichens gemma after fermenting after fermenting to the bacillus licheniformis of activation
Bacillus fermentation liquid is inoculated in the first fermentation medium, expands culture；The inoculum concentration of the lichen bacillus ferments liquid accounts for
The percent by volume of first fermentation medium is preferably 5%；The temperature for expanding culture is preferably 30~35 DEG C, more preferably
32~34 DEG C；The time for expanding culture is preferably 3~5d, reaches (spectrophotometric determination until strain logarithmic phase
OD600, using the culture medium that is not inoculated with as blank control, about 10~2,000,000,000 cfu/mL of bacterium number)；The equipment for expanding culture
Preferably fermentor.The present invention is preferred with ventilation and stirring in expanding incubation；Into in culture for 24 hours, ventilatory capacity
Preferably 10~20m3/ h, more preferably 15m3/h；After culture for 24 hours, ventilatory capacity is preferably 15~24m3/ h, more preferably 20m3/
h；The frequency of the stirring is preferably 1~2 time/h, and the time stirred every time is preferably 10~20min, more preferably 15min.
After obtaining bacillus licheniformis agent, gel-shaped series bacillus microbial inoculum and organic carrier, the present invention is by lichens bud
Spore bacillus microbial inoculum, gel-shaped series bacillus microbial inoculum and organic carrier are mixed, and secondary salinization soil remediation microbial inoculum is obtained.
In the present invention, the bacillus licheniformis agent and gel-shaped series bacillus microbial inoculum total volume and the organic carrier gross mass
Ratio be preferably 1mL:8~10g, more preferably 1mL:9g；The mixed temperature is preferably 25 DEG C~30 DEG C, more preferably
28℃；The present invention is not particularly limited the mixed time, is subject to and is uniformly mixed.
The present invention also provides secondary salinization soil remediation microbial inoculums described in above scheme in secondary salinization soil reparation
In application, comprising the following steps: the secondary salinization soil remediation microbial inoculum is applied to secondary salinization soil to be repaired；
The mode of the application preferably includes that cave is applied and/or spread fertilizer over the fields；When the mode of the application is applied for cave, the secondary salinization
The amount of application of soil remediation microbial inoculum is preferably the cave 80~120g/, more preferably the cave 100g/；When the mode of the application is to spread fertilizer over the fields
When, the amount of application of the secondary salinization soil remediation microbial inoculum is preferably 20~150kg/666.7m2, more preferably 30~
100kg/666.7m2, most preferably 50~80kg/666.7m2。
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
A kind of secondary salinization soil remediation microbial inoculum of embodiment 1
1. culture medium is prepared:
1) activation medium
Bacillus licheniformis: peptone 10g, beef extract 3g, NaCl 5g, agar 18g, distilled water 1L, pH7.0.
Gel-shaped series bacillus: sucrose 10g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium carbonate
1g, agar powder 20g (solid medium), distilled water 1L, pH 7.0.
2) fermentation medium:
Gel-shaped series bacillus: starch 5.5g, yeast powder 1.579g, bean cake powder 5.76g, magnesium sulfate 1.4g, phosphoric acid hydrogen
Dipotassium 2.0g, calcium carbonate 8.5g, sodium chloride 0.2g, pH 7.0.
Bacillus licheniformis: sucrose 15g, soy meal 10g, potassium dihydrogen phosphate 1g, zinc sulfate 0.1g, pH 6.5.
3) expand culture medium: the same fermentation medium of culture medium.
2. prepared by bacterium solution:
1) actication of culture: by function bacterium (gel-shaped series bacillus (CGMCC No.3995) and the lichens bud of cryo-conservation
Spore bacillus (CGMCC No.10741) streak inoculation is into first cell culture medium, for 24 hours in 28 DEG C of culture activation.
2) function bacterium after being activated the step 1) is inoculated into secondary medium, in 28 DEG C of shake flask fermentation 48h.
3) fermentation liquid of the step 2) is inoculated into the fermentor equipped with culture medium by 5% and is fermented, temperature is
30℃.3d is cultivated, (spectrophotometric determination OD600, using the culture medium that is not inoculated with as blank pair until reaching strain logarithmic phase
According to bacterium number about 1,000,000,000 cfu/mL).The process of culture is ventilated, into culture for 24 hours, ventilatory capacity 10m3/ h, culture
After for 24 hours, ventilatory capacity 15m3/ h, culture carry out under stiring, and the frequency of stirring is 1 time/h, and the time stirred every time is
10min。
4) two kinds of function bacteriums are pressed into the resulting fermentation liquid of step 3), with the ratio (bacillus licheniformis about 30 of 1:1 (V/V)
Hundred million cfu/mL, about 1,500,000,000 cfu/mL of gel-shaped series bacillus) it is mixed, obtain mixed bacteria liquid.
3. organic carrier adsorbs:
The agricultural wastes such as mushroom slag, bean cake powder or stalk are crushed with chain crusher in advance, with 80 mesh extension sets
It is sieved.The rotatable culture medium sterilizer of stainless steel is poured into, steam enters autoclave collet, carries out heating heating, heats
When to 121 DEG C, temperature 30min is kept, then leads to condensed water in collet and cools down, 25 DEG C are down to, by carrier and bacterium solution
The ratio of 9g:1mL (W/V) adds bacterium solution into sterilizer, and carries out Stirring, stirs 30min, after the completion of stirring, passes through
Autoclave reversion, pours out material.
A kind of secondary salinization soil remediation microbial inoculum of embodiment 2
1. culture medium is prepared:
1) activation medium
Bacillus licheniformis: peptone 10g, beef extract 3g, NaCl 5g, agar 18g, distilled water 1L, pH7.4.
Gel-shaped series bacillus: sucrose 10g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, sodium chloride 0.2g, calcium carbonate
1g, agar powder 20g (solid medium), distilled water 1L, pH 7.5.
2) fermentation medium:
Gel-shaped series bacillus: starch 5.5g, yeast powder 1.579g, bean cake powder 5.76g, magnesium sulfate 1.4g, phosphoric acid hydrogen
Dipotassium 2.0g, calcium carbonate 8.5g, sodium chloride 0.2g, pH 7.5.
Bacillus licheniformis: sucrose 15g, soy meal 10g, potassium dihydrogen phosphate 1g, zinc sulfate 0.1g, pH7.0.
4) expand culture medium: the same fermentation medium of culture medium.
2. prepared by bacterium solution:
1) actication of culture: by function bacterium (gel-shaped series bacillus (CGMCC No.3995) and the lichens bud of cryo-conservation
Spore bacillus (CGMCC No.10741) streak inoculation is into first cell culture medium, for 24 hours in 30 DEG C of culture activation.
2) function bacterium after being activated the step 1) is inoculated into secondary medium, in 30 DEG C of shake flask fermentation 48h.
3) fermentation liquid of the step 2) is inoculated into the fermentor equipped with culture medium by 5% and is fermented, temperature is
35℃.5d is cultivated, (spectrophotometric determination OD600, using the culture medium that is not inoculated with as blank pair until reaching strain logarithmic phase
According to bacterium number about 2,000,000,000 cfu/mL).The process of culture is ventilated, into culture for 24 hours, ventilatory capacity 20m3/ h, culture
After for 24 hours, ventilatory capacity 24m3/ h, culture carry out under stiring, and the frequency of stirring is 2 times/h, and the time stirred every time is
20min。
4) two kinds of function bacteriums are pressed into the resulting fermentation liquid of step 3), with the ratio (bacillus licheniformis about 40 of 1:1 (V/V)
Hundred million cfu/mL, about 2,500,000,000 cfu/mL of gel-shaped series bacillus) it is mixed, obtain mixed bacteria liquid.
3. organic carrier adsorbs:
The agricultural wastes such as mushroom slag, bean cake powder or stalk are crushed with chain crusher in advance, with 80 mesh extension sets
It is sieved.The rotatable culture medium sterilizer of stainless steel is poured into, steam enters autoclave collet, carries out heating heating, heats
When to 121 DEG C, temperature 30min is kept, then leads to condensed water in collet and cools down, 30 DEG C are down to, by carrier and bacterium solution
The ratio of 9g:1mL (W/V) adds bacterium solution into sterilizer, and carries out Stirring, stirs 30min, after the completion of stirring, passes through
Autoclave reversion, pours out material.
3 strain enzyme-producing vigor situation of embodiment
The fermentation liquid of fermentation for 24 hours is taken, 12000rpm is centrifuged 15min in 4 DEG C of refrigerated centrifuges, removes thallus, takes supernatant
(crude enzyme liquid) carries out enzyme activity detection.Blank is using the crude enzyme liquid of 100 DEG C of inactivations as control.
Nitrate reduction enzyme activity in bacterial strain fermentation liquor is detected with reference to the method for ROMANA.SIDDIQUI et al.:
After 50mM 2-morpholine ethane sulfonic acid (MES, pH 6.5) buffer, 30mM potassium nitrate and the preheating of 1mM sodium formate, in 37 DEG C rapidly
It is reacted with the fermentation liquid of equivalent, terminates reaction with the nitrite of equivalent after 10min, be measured at 436nm.
The method that nitrite reductase vigor in bacterial strain fermentation liquor refers to MARTINEZ-ESPINOSA RM et al..
0.1M phosphate buffer (pH6.5), 0.1M NaCl, 0.1M sodium nitrite, 0.1M methyl viologen (MV), 0.1M connect two sulfurous
Sour sodium and the mixing of 0.1M sodium thiosulfate, are rapidly added crude enzyme liquid after 30 DEG C of preheatings, react 10min, acutely terminate after concussion anti-
It answers.Griess reagent (for inspection by attributes nitrite anions) colour developing is taken, colorimetric method measures the variation of nitrite at 538nm.
The above enzyme activity is equal is defined as: enzyme amount needed for 1 μ g substrate of reduction is defined as 1 enzyme activity unit per minute, i.e.,
1U=1 μ g/min.
Measurement result is shown, is removed in the fermentation liquid of thallus, gel-shaped series bacillus nitrate reductase and nitrous acid
Salt reductase vitality is respectively 5.3U/mL and 8.9U/mL.Bacillus licheniformis nitrate reductase and nitrite reductase are living
Power is respectively 8.1U/mL and 10.2U/mL.The enzymatic activity highest of composite bacterial solution, nitrate reductase and nitrite reductase are living
Power is respectively 10.3U/mL and 11.7U/mL.Illustrate that the enzymatic productivity of combination strain is higher.Experimental result is referring to Fig. 1.
4 bacterial strain of embodiment improves the hardened situation of secondary salinization soil
Exocellular polysaccharide promotes soil agreegate to be formed, and improves the hardened problem of secondary salinization soil.
Using the method for soil column leaching, the surface layer the 1~20cm salinized soil of Zhangzhou City Xiangcheng District is taken, respectively by gel-shaped class bud
Spore bacillus (CGMCC No.3995) bacterium solution and bacillus licheniformis (CGMCC No.10741) press the inoculum concentration and soil of 5% (V/W)
Earth stirs and evenly mixs, and is packed into pvc pipe, wraps up pvc pipe mouth with 8 layers of sterile gauze, is monitored with tensiometer, controls moisture 20%
Left and right.After 2 months, soil (detection of soil granular analyzer) is sieved using artificial wet screening, the frequency of oscillation of sieve for 40 times/
Min, sieve aperture are 2,1,0.5,0.25 and 0.053mm.Soil sieve is divided into: diameter > 2mm macro aggregate, 2~0.25mm
Small agglomerates, 0.25~0.053mm microaggregate and 0.053 four ranks of glutinous sand grains below.Experimental result is referring to fig. 2.
In terms of test result, two plants of bacterium are all conducive to the formation of the hardened soil agreegate of salination, and gel-shaped class gemma
The ability that bacillus forms aggregate is better than bacillus licheniformis, and the combination of two bacterium is better than single bacterial strain function and effect, soil water stability
Aggregate (aggregate is generally divided into > 0.25mm water-stable aggregate and < 0.25mm microaggregate) is mentioned than blank control group
It is high by 30.2%.
5 potted plant experiment restoration facilities secondary salinization soil of embodiment
It is sand soil for examination soil, nitrate nitrogen content 0.68g/kg, total salt content 5.91g/kg belong to moderate soil
Salination (concrete condition is referring to table 1).Every basin fills soil 2kg, and the secondary salinization soil remediation microbial inoculum of embodiment 1 is applied using cave
Mode apply in surface soil, soil nitrate-N, organic matter and EC value are measured after sowing amount about 100g, 40d.
Table 1 is for trying soil regime
Test sets four processing, and following design treatment: (similarly hereinafter) is pressed in processing 1, processing 2, processing 3 and processing 4
1. handling 1: blank control (CK1)
2. handling 2: conventional fertilizer application (CK2)
3. handling 3: conventional fertilizer application+matrix (CK3)
4. handling 4: conventional fertilizer application+soil remediation microbial inoculum
Test result: the result shows that, after secondary salinization soil remediation microbial inoculum is added, compared with the control group, nitrate nitrogen contains
Amount reduces 48%, and the content of organic matter improves 52.8%, and soil EC value reduces 28.4%.(experimental data is referring to table 2) can
See that soil remediation microbial inoculum effectively converts the nitrate nitrogen in soil, improve the content of organic matter, reduce soil EC value, improves soil ring
Border.
Influence of 2 different disposal of table to potting soil
6 facility plastic greenhouse restoration facilities pakchoi secondary salinization soil of embodiment
It is sand soil (concrete condition is referring to table 3) for examination soil, canopy age 2 years content of soil nitrate-N are 0.62g/kg.
Every 666.7m2With the secondary salinization soil remediation microbial inoculum of 25kg embodiment 1, uniformly spread fertilizer over the fields in site preparation.After 56d, observe small
Chinese cabbage growing way, and measure content of soil nitrate-N and total salt content, yield of pakchoi and nitrate content.
Table 3 is tested preceding for trying soil regime
Test sets four processing, and following design treatment: (similarly hereinafter) is pressed in processing 1, processing 2, processing 3 and processing 4
1. handling 1: blank control (CK1)
2. handling 2: conventional fertilizer application (CK2)
3. handling 3: conventional fertilizer application+matrix (CK3)
4. handling 4: conventional fertilizer application+soil remediation microbial inoculum
Test result is referring to 4~table of table 7: the result shows that, after secondary salinization soil remediation microbial inoculum is added, nitrate nitrogen content
49.6% is reduced than blank control group, total salt content reduces 18.0%, and Nitrate Content in Pakchoi is reduced than conventional fertilizer application
27.2%, output increased 11.8%.It can be seen that soil remediation microbial inoculum effectively repairs secondary salinization, while promoting to make produce
Amount improves crop quality.
Influence of 4 different disposal of table to content of soil nitrate-N
Influence of 5 different disposal of table to soil soluble salt content
Influence of 6 different disposal of table to pakchoi plant nitrate content
7 different disposal yield of pakchoi situation table of table
7 facility plastic greenhouse of embodiment repairs cucumber secondary salinization soil
For examination soil be sandy loam, greenhouse age 5 be 5 years, nitrate nitrogen content be 0.9g/kg (for examination soil regime referring to
Table 8).Before transplanting, every 666.7m2Cave is carried out with the secondary salinization soil remediation microbial inoculum of 125kg embodiment 2 to apply, and repairs 57d
Afterwards, content of soil nitrate-N and the content of organic matter, cucumber yield and nitrate content are harvested and measure, experimental result is referring to table 9
~table 12.
Test result is shown, compared with conventional fertilizer application, after applying soil remediation microbial inoculum, and the nitrate nitrogen content of soil and solvable
Property total salt content reduce 51.7% and 57.4% respectively, the nitrate content in cucumber reduces 17.6%, volume increase 9.9%.
Table 8 is tested preceding for trying soil regime
Test sets four processing, and following design treatment: (similarly hereinafter) is pressed in processing 1, processing 2, processing 3 and processing 4
1. handling 1: blank control (CK1)
2. handling 2: conventional fertilizer application (CK2)
3. handling 3: conventional fertilizer application+matrix (CK3)
4. handling 4: conventional fertilizer application+soil remediation microbial inoculum
Influence of 9 different disposal of table to content of soil nitrate-N
Influence of 10 different disposal of table to soil soluble salt content
Influence of 11 different disposal of table to cucumber nitrate content
Influence of 12 different disposal of table to cucumber yield
As seen from the above embodiment, secondary salinization soil remediation microbial inoculum provided by the invention repairs secondary salinization soil
Significant effect.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of secondary salinization soil remediation microbial inoculum, the raw material including following volumes part: bacillus licheniformis agent 0.5~
1.5 parts and 0.5~1.5 part of gel-shaped series bacillus microbial inoculum；
The living bacteria count of bacillus licheniformis is 30~4,000,000,000 cfu/mL in the bacillus licheniformis agent；
The concentration of gel-shaped series bacillus is 15~2,500,000,000 cfu/mL in the gel-shaped series bacillus microbial inoculum；
The deposit number of the bacillus licheniformis is CGMCC No.10741；The deposit number of the gel-shaped series bacillus
For CGMCC No.3995.
2. microbial inoculum according to claim 1, which is characterized in that the microbial inoculum further includes organic carrier；The organic carrier
Including agricultural wastes.
3. microbial inoculum according to claim 2, which is characterized in that the organic carrier includes in mushroom slag, bean cake powder and stalk
One or more.
4. microbial inoculum according to claim 2 or 3, which is characterized in that the partial size of the organic carrier is 60~100 mesh.
5. microbial inoculum according to claim 4, which is characterized in that the bacillus licheniformis agent and gel-shaped class gemma bar
The ratio of bacteria agent total volume and the organic carrier gross mass is 1mL:8~10g.
6. secondary salinization soil remediation microbial inoculum described in Claims 1 to 5 any one is in secondary salinization soil reparation
Using, comprising the following steps: the secondary salinization soil remediation microbial inoculum is applied to secondary salinization soil to be repaired.
7. application according to claim 6, which is characterized in that the mode of the application includes that cave is applied and/or spread fertilizer over the fields.
8. application according to claim 7, which is characterized in that when the mode of the application is applied for cave, the secondary salt
The amount of application of stain soil remediation microbial inoculum is the cave 80~120g/.
9. application according to claim 7, which is characterized in that when the mode of the application is to spread fertilizer over the fields, the secondary salt
The amount of application of stain soil remediation microbial inoculum is 20~150kg/666.7m2。
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