CN115160077A - Preparation and application of soil remediation microbial inoculum for continuous cropping obstacles - Google Patents
Preparation and application of soil remediation microbial inoculum for continuous cropping obstacles Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention relates to a continuous cropping obstacle soil remediation microbial inoculum and application thereof, wherein the continuous cropping obstacle soil remediation microbial inoculum comprises the following raw materials in parts by weight: 30-50 parts of humic acid, 0-20 parts of mushroom residues, 10-20 parts of turf, 0-20 parts of vermiculite and 15-25 parts of functional microbial inoculum. The mushroom dregs are preferably mushroom dregs. The functional microbial inoculum comprises five fermentation microbial inoculants of bacillus subtilis, trichoderma harzianum, bacillus megaterium, bacillus mucilaginosus and metarhizium anisopliae. The functional bacteria selected by the invention can quickly become dominant flora in soil, particularly in the rhizosphere of crops, play the roles of inhibiting bacteria and preventing insects, improving plant system resistance, fixing nitrogen, dissolving phosphorus and the like, have safe and environment-friendly carrier, can avoid soil hardening, and enhance the air permeability and water retention of the soil. The invention is applied to the conditions of poor crop growth condition, reduced yield, deteriorated quality, aggravated pest and disease damage and the like caused by continuous cropping, and can effectively repair and improve the continuous cropping obstacle soil, so that the quality of the crops is improved and the yield of the crops is increased.
Description
Technical Field
The invention belongs to the field of agricultural microbial agents, and particularly relates to preparation and application of a soil remediation microbial agent for continuous cropping obstacles.
Background
The continuous cropping obstacle refers to the occurrence of abnormal growth and development of crops caused by the continuous cultivation of the same crop or nearby crops on the same soil, such as poor growth condition, low yield, poor quality, and increased occurrence of plant diseases and insect pests. Because the agricultural cultivation in China has the characteristics of regionality and continuous cropping, more than 80% of the soil in China has the problem of continuous cropping obstacles, the phenomenon of continuous cropping obstacles commonly exists in crops such as grains, vegetables, fruit trees, medicinal materials, flowers and the like, and the less serious people cause yield reduction and even no more than the serious people. The continuous cropping obstacles mainly include 1. Soil microbial structure unbalance, pathogenic microorganism increase, beneficial microorganism decrease, and soil-borne diseases and insect pests gradually aggravate, such as soft rot and fungal stalk disease of Brassicaceae; wilt, root rot and damping-off of solanaceous fruits; damping-off, epidemic and blight of melons, and stubble density of insect pests such as spider mites, trialeurodes vaporariorum, aphids, thrips, nematodes and the like are also increased year by year. 2. The physical and chemical properties of soil become worse, the nutrients are disordered, and the salt content and pH imbalance become worse along with the prolonging of the cultivation age, so that the surface soil layer is hardened. 3. Because of allelopathy, autotoxicant secreted from crop roots or residues is gradually accumulated to hinder crop growth.
Compared with agricultural measures and chemical prevention and control, the soil remediation microbial inoculum for solving the continuous cropping obstacle problem has the advantages of energy conservation, high efficiency, economy, strong durability, stable improvement effect and the like, and integrates economic, environmental and ecological benefits. In the existing patent of the soil remediation microbial inoculum for the continuous cropping obstacle, the main direction is to add nutrient elements and prevent and control soil-borne diseases without involving insect pests, and eggs of insect pests such as spider mites, whiteflies, aphids, thrips, grubs, scarab, nematodes and the like can overwinter in soil, and the insect pests can gradually rise along with the continuous cropping. And the addition of some nutrient elements causes the soil to have certain limitation, is not suitable for general crops, and can increase the volume weight, the salinity and the alkalinity of the soil and the like.
Aiming at three problems of aggravated diseases and insect pests, unbalanced nutrients and autotoxic substance accumulation which are main reasons causing continuous cropping obstacles, the invention develops a soil remediation microbial inoculum based on the micro-ecological theory of plants, screens beneficial microbes for promoting growth from the root system or rhizosphere soil of a healthy soil plant with serious continuous cropping obstacles, establishes a micro-ecological system microbial resource library, screens out the beneficial microbes for efficiently promoting growth, preventing diseases and resisting stress from the micro-ecological system microbial resource library, and determines the fermentation conditions and carriers of strains as the effective components of the soil remediation microbial inoculum through the research and development of a strain fermentation process and a preparation processing process to convert the strains into soil remediation microbial inoculum products. The fertilizer can resist plant diseases and insect pests, promote the growth of crops, increase soil nutrients and effectively relieve the problem of continuous cropping obstacle of soil.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provide a continuous cropping obstacle soil remediation microbial inoculum which can resist diseases and insect pests, promote the growth of crops and increase soil nutrients.
The technical scheme adopted by the invention for solving the technical problems in the prior art is as follows: a continuous cropping obstacle soil remediation microbial inoculum comprises the following raw materials in parts by weight: 30-50 parts of humic acid, 0-20 parts of mushroom residues, 10-20 parts of turf, 0-20 parts of vermiculite and 15-25 parts of functional microbial inoculum.
In the continuous cropping obstacle soil remediation microbial inoculum, the mushroom residues are preferably mushroom residues.
In the continuous cropping obstacle soil remediation microbial inoculum, the functional microbial inoculum further comprises five fermentation microbial inoculants of bacillus subtilis, trichoderma harzianum, bacillus megaterium, bacillus mucilaginosus and metarhizium anisopliae. Wherein the bacillus subtilis is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation number is CGMCC NO.7850; the Trichoderma harzianum is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms, and the preservation numbers are CGMCC NO.7861 respectively; the metarhizium anisopliae is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and the preservation numbers are respectively CGMCC NO.7848.
In the continuous cropping obstacle soil remediation microbial inoculum, further, the functional microbial inoculum comprises the following components in parts by weight: 40-60 parts of bacillus subtilis, 2-5 parts of trichoderma harzianum, 20-30 parts of bacillus megaterium, 10-20 parts of bacillus mucilaginosus and 2-5 parts of metarhizium anisopliae.
A preparation method of a continuous cropping obstacle soil remediation microbial inoculum comprises the following steps:
1. and (3) fermenting mushroom residues: crushing mushroom dregs by a chain crusher, sieving by a 50-mesh sieve, stacking and fermenting, and turning over once every a period of time according to specific conditions. Generally fermenting for about one week.
2. Fermenting strains: respectively carrying out liquid fermentation on bacillus subtilis, bacillus megatherium and bacillus mucilaginosus, and respectively carrying out solid fermentation on trichoderma harzianum and metarhizium anisopliae.
3. According to the proportion, adsorbing the fermentation liquor by using turf, adding humic acid, mushroom residues and vermiculite, uniformly stirring in a stirrer, preparing into powder or granules, and drying to obtain the soil remediation microbial inoculum.
In conclusion, the invention has the following advantages and positive effects:
the five functional bacteria selected by the invention have different effects, and the bacillus subtilis can compete with pathogenic microorganisms for nutrients around the plant body by successfully colonizing in the rhizosphere, the body surface or the body, and can secrete some bacteriostatic substances to inhibit the growth of the pathogenic bacteria around the plant body. Meanwhile, the bacillus subtilis can induce the resistance of a plant system, so that the resistance of a plant body is improved, and the aim of resisting the invasion of pathogenic microorganisms is fulfilled. Trichoderma harzianum mainly utilizes the heavy parasitic action, the antibiotic action and the competitive action to prevent and treat pathogenic bacteria; the bacillus megaterium provides nutrient components for host plants through nitrogen fixation and phosphorus dissolution, and degrades organic phosphorus in soil; the bacillus mucilaginosus can decompose original ecological mineral substances of aluminosilicate such as potassium feldspar, mica and the like, so that insoluble K, P, si and the like in soil are converted into soluble elements for plants to utilize, and medium and trace elements such as soluble calcium, sulfur, magnesium, iron, molybdenum, manganese and the like are released, thereby not only improving the soil fertility, but also effectively improving the utilization rate of a chemical fertilizer. The metarhizium anisopliae can well colonize plant roots and bodies, has a wide pest control range, and has good effects of controlling spider mites, whiteflies, aphids, thrips, grubs, scarab, nematodes and the like. The five functional bacteria can be symbiotically propagated in soil, particularly in the rhizosphere of crops, have complementary action advantages and can be rapidly propagated in the soil to form dominant flora, so that the effects of restoring the soil micro-ecological environment and improving the quality and the yield of the crops are achieved.
The soil remediation microbial inoculum carrier is safe and environment-friendly, effectively solves the comprehensive treatment of reduction, harmlessness and reclamation of agricultural wastes, can effectively avoid soil hardening, and enhances the air permeability and water retention of soil. The preparation method is simple in preparation process, suitable for industrial production and suitable for large-area use in planting of fruits, vegetables, traditional Chinese medicinal materials and the like.
The application of the soil remediation microbial inoculum comprises the following method,
(1) Basal application: 40kg of soil remediation microbial inoculum is used per mu, and the soil remediation microbial inoculum can be mixed with organic fertilizer for application and then covered with soil;
(2) Hole application: applying 20-40 kg of soil remediation microbial inoculum per mu;
(3) Strip application or broadcast application: using 40kg of soil remediation microbial inoculum for each mu;
(4) Seed dressing and root dipping: adding a proper amount of soil remediation microbial inoculum into clear water to be pasty, uniformly stirring with seeds, slightly airing, sowing and covering soil.
Detailed Description
In order to further understand the contents, features and effects of the present invention, the following examples are illustrated, and the following detailed descriptions are given:
example 1
The continuous cropping obstacle soil remediation microbial inoculum comprises the following raw materials in parts by weight:
40 parts of humic acid, 20 parts of turf, 20 parts of vermiculite and 20 parts of functional microbial inoculum.
In the continuous cropping obstacle soil remediation microbial inoculum, the functional microbial inoculum further comprises five fermentation microbial inoculants of bacillus subtilis, trichoderma harzianum, bacillus megaterium, bacillus mucilaginosus and metarhizium anisopliae.
In the continuous cropping obstacle soil remediation microbial inoculum, further, the functional microbial inoculum comprises the following components in parts by weight: 50 parts of bacillus subtilis, 5 parts of trichoderma harzianum, 25 parts of bacillus megaterium, 15 parts of bacillus mucilaginosus and 5 parts of metarhizium anisopliae.
A preparation method of a continuous cropping obstacle soil remediation microbial inoculum comprises the following steps:
respectively carrying out liquid fermentation on bacillus subtilis, bacillus megaterium and bacillus mucilaginosus, and respectively carrying out solid fermentation on trichoderma harzianum and metarhizium anisopliae, wherein the specific culture medium is as follows.
B, bacillus subtilis activation culture medium: 0.3% of beef extract, 0.5% of peptone, 0.5% of NaCl0 and 2% of agar, and the pH value is 7.0-7.5. Shake flask culture medium: 0.3% of beef extract, 0.7% of peptone, 0.5% of sodium chloride and 7.0% of pH value. Fermentation tank culture medium: 2 percent of soybean meal, 0.5 percent of fish meal, 1.8 percent of corn flour, 0.4 percent of starch, 0.06 percent of magnesium sulfate, 0.5 percent of dipotassium phosphate, 0.025 percent of natural enemy and 7.0 to 7.5 percent of pH value.
The fermentation process of the bacillus megaterium is the same as that of the bacillus subtilis.
Activating culture medium of bacillus mucilaginosus: 0.5% of starch, 0.5% of sucrose, 0.05% of yeast powder, 0.01% of calcium carbonate, 0.05% of calcium sulfate, 0.05% of magnesium sulfate heptahydrate, 2% of agar and 7.0-7.5 of pH value. Shake flask culture medium: molasses 2%, soybean meal 0.5%, calcium carbonate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.5%, natural killer 0.025%, and pH value 7.0-7.5. Fermentation tank culture medium: molasses 2%, soybean meal 0.5%, calcium carbonate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.5%, natural killer 0.025%, and pH value 7.0-7.5.
Trichoderma harzianum activation PDA medium was used: 20% of potato, 2% of glucose and 2% of agar, and the pH value is 7.0-7.5. Solid medium: wheat bran 90% and corn flour 10%.
The fermentation process of the metarhizium anisopliae is the same as that of trichoderma harzianum.
According to the proportion, adsorbing the bacterial liquid by using turf, adding humic acid and vermiculite, uniformly stirring and mixing in a stirrer, detecting, and packaging qualified finished products to obtain the soil remediation microbial inoculum.
TABLE 1 technical indexes of soil remediation microbial inoculum powder product
| Appearance of the product | Powdered and loose |
| Effective viable count/(CFU)/[ hundred million/g (mL)]≥ | 10.0 |
| Number of fungi/[ piece/g (mL)] ≤ | 3.0×10 6 |
| The rate of mixed bacteria/(%) is less than or equal to | 10.0 |
| Organic matter/(%) is not less than | 20 |
| pH value | 5.5-8.5 |
Example 2
The soil remediation microbial inoculum for the continuous cropping obstacle comprises the following raw materials in parts by weight:
40 parts of humic acid, 20 parts of mushroom residues, 20 parts of turf and 20 parts of functional microbial inoculum.
In the continuous cropping obstacle soil remediation microbial inoculum, the mushroom dregs are preferably mushroom dregs.
In the continuous cropping obstacle soil remediation microbial inoculum, the functional microbial inoculum further comprises five fermentation microbial inoculants of bacillus subtilis, trichoderma harzianum, bacillus megaterium, bacillus mucilaginosus and metarhizium anisopliae.
In the continuous cropping obstacle soil remediation microbial inoculum, further, the functional microbial inoculum comprises the following components in parts by weight: 50 parts of bacillus subtilis, 5 parts of trichoderma harzianum, 25 parts of bacillus megaterium, 15 parts of bacillus mucilaginosus and 5 parts of metarhizium anisopliae.
A preparation method of a continuous cropping obstacle soil remediation microbial inoculum comprises the following steps:
and (3) mushroom residue fermentation: crushing mushroom residue with a chain crusher, sieving with a 50-mesh sieve, stacking, fermenting, and turning over once every certain time according to specific conditions. Generally fermenting for about one week.
Respectively carrying out liquid fermentation on bacillus subtilis, bacillus megatherium and bacillus mucilaginosus, and respectively carrying out fermentation on trichoderma harzianum and metarhizium anisopliae, wherein the specific culture medium is as follows.
B, activating culture medium of bacillus subtilis: 0.3% of beef extract, 0.5% of peptone, 0.5% of NaCl0.5% of agar and 7.0-7.5% of pH value. Shake flask culture medium: 0.3% of beef extract, 0.7% of peptone, 0.5% of sodium chloride and 7.0% of pH value. Fermentation tank culture medium: 2 percent of soybean meal, 0.5 percent of fish meal, 1.8 percent of corn flour, 0.4 percent of starch, 0.06 percent of magnesium sulfate, 0.5 percent of dipotassium phosphate, 0.025 percent of natural enemy and 7.0 to 7.5 percent of pH value.
The fermentation process of the bacillus megaterium is the same as that of the bacillus subtilis.
Activating culture medium of bacillus mucilaginosus: 0.5% of starch, 0.5% of sucrose, 0.05% of yeast powder, 0.01% of calcium carbonate, 0.05% of calcium sulfate, 0.05% of magnesium sulfate heptahydrate, 2% of agar and 7.0-7.5 of pH value. Shake flask culture medium: molasses 2%, soybean meal 0.5%, calcium carbonate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.5%, sodium hypochlorite 0.025%, and pH 7.0-7.5. Fermentation tank culture medium: molasses 2%, soybean meal 0.5%, calcium carbonate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.5%, natural killer 0.025%, and pH value 7.0-7.5.
Trichoderma harzianum activation PDA medium was used: 20 percent of potato, 2 percent of glucose and 2 percent of agar, and the pH value is 7.0-7.5. Solid medium: wheat bran 90% and corn flour 10%.
The fermentation process of the metarhizium anisopliae is the same as that of trichoderma harzianum.
According to the proportion, the bacteria liquid is adsorbed by turf, then humic acid and mushroom residues are added, the mixture is placed in a stirrer to be stirred and mixed uniformly, the mixture is granulated by a granulator, and the finished product is packaged after the detection is qualified, so that the soil remediation microbial inoculum is obtained.
TABLE 2 technical indexes of granular soil remediation microbial inoculum product
| Appearance of the product | Granular, no obvious impurity |
| Number of active bacteria/(CFU)/[ hundred million/g (mL)] ≥ | 10.0 |
| Number of fungi and fungi/[ piece/g (mL)] ≤ | 3.0×10 6 |
| The rate of mixed bacteria/(%) is less than or equal to | 10.0 |
| Organic matter/(%) is not less than | 20 |
| pH value | 5.5-8.5 |
The continuous cropping obstacle soil remediation microbial inoculum has a good effect on continuous cropping obstacles of various crops.
Experiment 1: prevention and treatment experiment of soil remediation microbial inoculum on potato scab
The experiment is carried out on a land where potatoes are planted in Jinshan town of Guyang county, baotou City, autonomous region of inner Mongolia in 2020 and 5-10 months continuously for 8 years, and two treatments are set up in total: conventional fertilization, soil remediation microbial inoculum and conventional fertilization. Each treatment set 3 cells, each cell area 30m 2 . The soil remediation microbial inoculum has the effects on the control effect of potato scab, the yield and the influence on the soil nutrients of potato rhizosphere as shown in the following tables 3 and 4.
TABLE 3 prevention and treatment effects of soil remediation microbial inoculum on potato scab
| Experimental treatment | Disease index (%) | Control effect (%) | Yield (kg/mu) | Yield increase (%) |
| Conventional fertilization | 60.11 | - | 2603.87 | - |
| Soil remediation microbial inoculum and conventional fertilization | 25.61 | 57.39 | 3221.77 | 23.73 |
TABLE 4 influence of soil remediation microbial inoculum on soil nutrient at potato rhizosphere
| Treatment of | pH value | Organic matter (g/kg) | Total nitrogen (g/kg) | Available phosphorus (mg/kg) | Quick-acting potassium (mg/kg) |
| Conventional fertilization | 7.65 | 20.036 | 25.63 | 65.32 | 358.71 |
| Soil remediation microbial inoculum and conventional fertilization | 7.32 | 24.669 | 38.95 | 79.36 | 563.21 |
Experiment 2: experiment for preventing and treating beet root rot by soil remediation microbial inoculum
The experiment is carried out on land blocks for continuously planting 6-year beet in Xingguan spring county in autonomous city of inner Mongolia autonomous region in 2021, and two treatments are set up in total: conventional fertilization and soil remediation microbial inoculum + conventional fertilization. Each process is provided with 3 cells, each cell having an area of 30m 2 . The soil remediation microbial inoculum has the effects on the control effect and yield of beet root rot and the influence on the nutrients of beet rhizosphere soil as shown in tables 5 and 6 below.
TABLE 5 soil remediation microbial inoculum for preventing and treating root rot of beetroot
TABLE 6 influence of soil remediation Agents on sugar beet rhizosphere soil nutrients
| Test treatment | pH value | Organic matter (g/kg) | Total nitrogen (g/kg) | Available phosphorus (mg/kg) | Quick-acting potassium (mg/kg) |
| Conventional fertilization | 7.36 | 25.30 | 1.93 | 120.85 | 231.62 |
| Soil remediation microbial inoculum and conventional fertilization | 7.19 | 28.22 | 2.03 | 153.64 | 365.16 |
Experiment 3: experiment for preventing and treating tetranychus cinnabarinus by soil remediation microbial inoculum
The experiment is carried out in a greenhouse for 4 years continuously planted in Yangao county of Shanxi province in 2021-10 months, the crop variety is strawberry beads, and two treatments are set: conventional fertilization and soil remediation microbial inoculum + conventional fertilization. Each process is provided with 3 cells, and each cell has an area of 60m 2 . The soil remediation microbial inoculum for controlling tetranychus cinnabarinus is shown in the following table 7.
TABLE 7 prevention and treatment effects of soil remediation microbial inoculum on tetranychus cinnabarinus of strawberries
Claims (9)
1. The soil remediation microbial inoculum for the continuous cropping obstacle is characterized by comprising the following raw materials in parts by weight: 30-50 parts of humic acid, 0-20 parts of mushroom residues, 10-20 parts of turf, 0-20 parts of vermiculite and 15-25 parts of functional microbial inoculum.
2. The continuous cropping obstacle soil remediation microbial inoculum according to claim 1, further comprising mushroom residues, preferably mushroom residues.
3. The continuous cropping obstacle soil remediation microbial inoculum according to claim 1, further comprising five fermentation microbial inoculants, namely bacillus subtilis, trichoderma harzianum, bacillus megaterium, bacillus mucilaginosus and metarhizium anisopliae.
4. The functional microbial inoculum according to claim 3, further comprising the following components in parts by weight: 40-60 parts of bacillus subtilis, 2-5 parts of trichoderma harzianum, 20-30 parts of bacillus megaterium, 10-20 parts of bacillus mucilaginosus and 2-5 parts of metarhizium anisopliae.
5. The continuous cropping obstacle soil remediation microbial inoculum according to claim 1, wherein the preparation method comprises the following steps:
(1) And (3) fermenting mushroom residues: crushing mushroom residue by using a chain crusher, sieving by using a 50-mesh sieving machine, then stacking and fermenting, and turning over once every a period of time according to specific conditions.
6. (2) Fermenting strains: and (3) fermenting strains: respectively carrying out liquid fermentation on bacillus subtilis, bacillus megaterium and bacillus mucilaginosus, and respectively carrying out solid fermentation on trichoderma harzianum and metarhizium anisopliae.
7. (3) According to the proportion, adsorbing the fermentation liquor by using turf, adding humic acid, mushroom residues and vermiculite, uniformly stirring in a stirrer, preparing into powder or granules, and drying to obtain the soil remediation microbial inoculum.
8. The application of the continuous cropping obstacle soil remediation microbial inoculum according to claim 1, wherein the continuous cropping obstacle soil remediation microbial inoculum comprises the following components: the soil remediation microbial inoculum is applied to the conditions of poor crop growth condition, reduced yield, deteriorated quality, aggravated pest and disease damage and the like caused by continuous cropping.
9. The application of the continuous cropping obstacle soil remediation microbial inoculum according to claim 6, comprising the following application method,
(1) Basal application: the soil remediation microbial inoculum is 40kg per mu, and can be mixed with organic fertilizer for application, and soil is covered after application;
(2) Hole application: applying 20 kg-40 kg of soil remediation microbial inoculum per mu;
(3) Strip application or broadcast application: 40kg of soil remediation microbial inoculum is used per mu;
(4) Seed dressing and root dipping: and adding a proper amount of soil remediation microbial inoculum into clear water to be pasty, uniformly mixing with the seeds, slightly airing, sowing, and covering soil.
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