CN106801024A - Alcaligenes faecalis Y1 and its application in soil-borne disease of crop is prevented and treated - Google Patents
Alcaligenes faecalis Y1 and its application in soil-borne disease of crop is prevented and treated Download PDFInfo
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Abstract
The present invention provides one plant of Alcaligenes faecalis Y1 and its application in soil-borne disease of crop is prevented and treated.The preserving number CGMCC NO.13510 of the Alcaligenes faecalis Y1, it is made into microbial bacterial agent obvious for the growth-promoting effect of increasing production of field sunflower, and its action effect is not suppressed by field soil condition, fertilizer application, can be applied together with chemical fertilizer in slight saline and alkaline farmland.Rotten disk that the microbial inoculum triggers to sunflower nuclear disk disease prevention effect reaches more than 95%, rust prevention effect up to 100%, sunflower volume increase 16% 30%, booth vegetable volume increase more than 30%.The microbial bacterial agent is prevented and treated China's farmland soil-borne disease and continuous cropping obstacle, and Continued Increasing Grain Yield and the ecological environment that protects the fields are significant.
Description
Technical field
The present invention relates to microbiology and plant disease biological and ecological methods to prevent plant disease, pests, and erosion technical field, specifically, it is related to one plant of Alcaligenes faecalis Y1
And its application in soil-borne disease of crop is prevented and treated.
Background technology
Raising and Facility Agriculture Development with crop yield level, soil-borne disease are protruded increasingly with Soil-sickness Problem,
Microorganism is continuously increased pathogenic soil, triggers crop failure even to have no harvest.Soil-borne disease has become agriculture peace with continuous cropping obstacle
The bottleneck of full production development.Continuous cropping 3 years, yield reduction 10%~20%, 4~5 years crop failures 40~50% of continuous cropping.If
Apply vegetables, potato, garlic, sunflower, rape, cotton, soybean, paddy rice, wheat, beet soil-borne disease to occur often, the underproduction
It is increasingly serious, influence peasant's increasing both production and income.China is subjected to serious soil-borne disease, the area of continuous cropping obstacle harm and is up to 500,000,000 mu.
Because soil-borne disease is aggravated, the pesticide volume that China uses every year is more than 1,300,000 tons, and Pesticide use amount is generally exceeded,
Average every mu is applied 931.3g, and one times is higher by than developed country.Receive about 16,000,000 hectares of the farmland of pollution by pesticides, main agriculture in the whole nation
Residues of pesticides exceeding standard rate in product is up to 20%.Soil-borne disease Prevention Technique is not solved effectively, biological control soil
It is to substitute the optimal selection of chemical pesticide to pass disease.
The strain of soil-borne disease biological control is generally that Trichoderma, streptomycete, pythium oligandrum bacterium, Bacillus etc. are preferred.Preventing and treating
The microbial bacterial agent product of soil-borne disease, the K84 of the product such as Australia of own commercialization, radiation dragon bacillus strain K84 exists
Just mass produced within 1973, for preventing and treating root knot；Gustafson companies in 1988 are in U.S.'s registration production A13 products
Kodiak, as the person that opens up boundary that crop biocontrol microorganisms are commercially produced.Using Pseudomonas fluorescens (P.fluorescens CHA0)
The Fungicidal formulations of productionYieldSoil fungi disease can be prevented and treated.Puli
The product of Mao Si Serratieaes (Serratia plymuthica HRO-C48) productionAnd actinomyces
(Streptomyces sp.HRO-71) productionPreventing and treating strawberry and potato biology verticillium wilt (Verticillium
dahliae).Trichoderma reesei G1 and Trichoderma viride G3 prevent and treat damping-off effect is significant.
The Trichodex (Trichoderma harzianum T39) that the Topshield (Trichoderma harzianum T22) and Israel that the U.S. develops are developed
It is the commercial preparation for preventing and treating fungal disease such as gray mold and the exploitation of Mycontrol companies of Israel
Trichoderma2000 is mainly used in preventing and treating Rhizoctonia solani Kuhn, Sclerotium rolfsii, phytophthora etc.；It is also more research that shield shell is mould
A kind of biocontrol microorganisms, shield shell is mould to have very strong suppression to make Sclerotinia and the Bulbus Allii Fistulosi corruption microbial plurality of plant diseases of bulbil
With.External existing commercial prod occurs now, the commercial preparation Coniothyrin of such as former Soviet Union's exploitation can be used for preventing and treating to
The sclerotiniose of day certain herbaceous plants with big flowers, the commercial preparation ntansWG of Germany's exploitation can be used to prevent and treat sclerotinite sclerotiniose of lettuce etc..In addition with
Mould (Gliocladium virens), the SoilGard succeeded in developing by the U.S., for preventing and treating samping off and root-rot for viscous broom
Deng the BiofoxC that Italy develops is exclusively used in preventing and treating Fusarium disease etc..Trichoderma bactericide (the raw bacterium of Chinese commodity
Disappear, also known as sterilization ling), it is mainly used in preventing and treating the downy mildew of various crops, 5406 antibiotic bacterias of China's exploitation are streptomyces, fixed
Entitled Streptomyces jingyangensis, it is resistant to plurality of plant diseases such as verticillium wilt pathogen, wilt, rhizoctonia solani, da mping-off fungis, and
And plant growth can be promoted.Antagonistic bacterium (Bacillus subtilis) large-scale demonstration promotes experiment and proves that Antagonistic Fungi is in water
Colonization ability is stronger on rice plants, and breeding is very fast, to banded sclerotial blight preventive effect up to 75%~85%, to false smut preventive effect up to 63.8%~
85.7%.Antagonistic Trichoderma and antagonistic bacterium mixed fermentation are made pulvis, have successfully prevented and treated the seedling of vegetable at protected field and muskmelon
Phase disease.The bacterium bactericide of other reports is used for preventing and treating the bacillus licheniformis of cucumber and Colletotrichumtabacum, withered grass gemma
Bacillus prevents and treats sugarcane black rot, and radiation Pseudomonas prevent and treat take-all etc..Trichoderma harzianum chlamydospore preparation is to Xinjiang cotton
Flower verticillium wilt large area experiment preventive effect reaches 60%, and volume increase 50%, velveteen length increases by 10%, shows good application preceding
Scape.
Sunflower has the characteristics such as Salt And Alkali Tolerance, barren-resistant, drought-resistant strong adaptability, and seed oil content is high, and oil is excellent, existing
The big oil crops in the world the 4th are turned into.Global sunflower planting area is 26,000,000 hm within 20022, sunflower seeds yield is 2400
Ten thousand t.It is reported that the t of 2014,2015 annual global sunflower seeds yield 1551.9 ten thousand.China's sunflower area is 113.1 ten thousand within 2004
hm2, total yield is 194.6 ten thousand t, the hm of China sunflower sown area 1,020,000 in 20072, total output is 192.8 ten thousand t, per unit area yield
1889.3kg/ha。
Sunflower continuous cropping causes soil nutrient particularly potassium element to consume excessively, and soil fertility is difficult to recover.Sunflower disease is such as
Sclerotiniose, rust, brown spot, downy mildew, leaf blight, and certain herbaceous plants with big flowers snout moth's larva, grub, black cutworm etc., can all be endangered because of continuous cropping and added
It is acute.Sclerotium blight of sunflower, also known as white rot, rotten disk disease, the yield and quality influence on sunflower is very big.
Sclerotium blight of sunflower occurs very extensively, to fall ill heavier, sunflower stem can be caused to roll over, floral disc and benevolence are rotted, to
Day certain herbaceous plants with big flowers production causes serious threat.Common are the rotten type of root-rot type, stem rot type, leaf, spend the type of rotten type 4, wherein root-rot type, flower
Rotten type is aggrieved heavy.Spend rotten type disease show as floral disc it is aggrieved after, there is water soaking mode scab in the disk back side.
Presently mainly chemical control:1. with 40% dimethachlon, 800~1000 times of liquid；2. with 50% thiophanate wettable powder
1000 times of liquid of agent；3. with 50% Sukeling, 500~1000 times of liquid.Prevention effect is not very good, and prevention effect is only 80%
Left and right, therefore, it is extremely important with the bio-feritlizer of continuous cropping obstacle that research and development prevent and treat soil-borne disease.
The content of the invention
The purpose of the present invention be directed to China's sunflower continuous cropping cause sclerotiniose and rust frequently to occur, the underproduction seriously even
The production problem of total crop failure, there is provided plant height effect prevents and treats Alcaligenes faecalis Y1 and its microorganism of preparation of sclerotium blight of sunflower and rust
Microbial inoculum.
It is a further object of the present invention to provide applications of the Alcaligenes faecalis Y1 in soil-borne disease of crop is prevented and treated.
In order to realize the object of the invention, the present invention is separated and purified and obtains from Heilongjiang Province of China Shuangcheng City agricultural land soil
One plant height effect prevents and treats Alcaligenes faecalis (Alcaligenes faecalis) Y1 of sclerotium blight of sunflower and rust, in being preserved in
State's Microbiological Culture Collection administration committee common micro-organisms center, address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology of the academy of sciences of state, postcode 100101, deposit number CGMCC NO.13510, preservation date December 28 in 2016
Day.
Mycology feature and 16S rDNA sequencing results (SEQ ID NO according to bacterial strain Y1:1), determine that bacterial strain Y1 is produced for excrement
Alkali bacterium.
The present invention also provides the microbial bacterial agent containing the Alcaligenes faecalis Y1.
Also contain additive and protective agent in microbial inoculum of the present invention.
Wherein, the additive is silica, precipitated calcium carbonate, kaolin, bentonite, powdered rice hulls, wheat husk powder, stalk
At least one in powder etc..
The protective agent is at least one in glycerine, defatted milk, vegetable oil, sodium alginate, shitosan etc..
The microbial inoculum that the present invention is provided is made up of the component of following weight portion：
1 part of Alcaligenes faecalis Y1 zymotic fluids；
Additive 0.5-3 parts；
Protective agent 0.01-0.05 parts；
Alcaligenes faecalis Y1 viable counts are 2 × 10 in the microbial inoculum8-5×109Cfu/g, preferably 5 × 108cfu/g。
The present invention also provides the Alcaligenes faecalis Y1 or described microbial inoculums in preventing and treating soil-borne disease of crop (sunflower sclerotium bacteria
Disease, rust of sunflower, booth vegetable continuous cropping obstacle) in application.
The pathogen of the soil-borne disease is including sclerotinite, Puccinia helianthi, pinch outs etc..
The present invention also provides application of the Alcaligenes faecalis Y1 or described microbial inoculums in crop yield is improved.
The crop includes booth vegetable.Preferably, the crop is including sunflower, garlic, muskmelon, tomato etc..
The present invention also provides application of the Alcaligenes faecalis Y1 or described microbial inoculums in agricultural Chemical Mixed Fertilizer is prepared.
The present invention also provides a kind of agricultural Chemical Mixed Fertilizer, is the microbial inoculum and nitrogenous fertilizer, phosphate fertilizer, potassium of Alcaligenes faecalis Y1 by active ingredient
Fertilizer or composite fertilizer's composition.
The active ingredient that the present invention is provided is increased production for the microbial bacterial agent of Alcaligenes faecalis Y1 for the growth-promoting of field sunflower
Substantially, and its action effect do not suppressed effect by field soil condition, fertilizer application, can in slightly saline and alkaline farmland, with
Chemical fertilizer is applied together.The rotten disk disease prevention effect that the microbial inoculum triggers to sunflower nuclear disk reaches more than 95%, rust prevention effect
Up to 100%, sunflower volume increase 16%-30%, booth vegetable volume increase more than 30%.The microbial bacterial agent is to China's farmland soil-borne disease
Evil and continuous cropping obstacle preventing and treating, Continued Increasing Grain Yield and the ecological environment that protects the fields are significant.
Brief description of the drawings
Fig. 1 is the effect of Y1 bacterial strain flat boards suppression pinch outs in the embodiment of the present invention 5.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.If not specializing, embodiment
In the conventional meanses that are well known to those skilled in the art of technological means used, it is raw materials used to be commercial goods.
The preparation of the Alcaligenes faecalis Y1 microbial inoculums of embodiment 1
1. the separation and purifying of Alcaligenes faecalis Y1
Separated from Heilongjiang Province of China Shuangcheng City agricultural land soil and purified obtain a plant height effect prevent and treat sclerotium blight of sunflower
With the bacterial strain Y1 of rust.
Mycology feature and 16S rDNA sequencing results (SEQ ID NO according to bacterial strain Y1:1), determine that bacterial strain Y1 is produced for excrement
Alkali bacterium.
2. the culture of Alcaligenes faecalis Y1
(1) composition (kg/t zymotic fluids) of culture medium
Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, dusty yeast 5kg,
(NH4)2SO43kg, K2HPO40.2kg, NaCl 2.5kg, MgSO4·7H2O 0.1kg, CaCO30.5kg, FeSO4
0.001kg, boric acid (1%) 1L, pH7.0, are prepared with water.
(2) fermented and cultured process
Alcaligenes faecalis Y1 is inoculated on potato sucrose (PDA) culture medium, 25~28 DEG C of culture 3d.Alcaligenes faecalis Y1 is turned
Access 1000mL triangular flasks, 26-28 DEG C, liquid fermentation and culture 36h under 220r/min.Then 50L seeds are accessed by 1% inoculum concentration
Tank, under the conditions of 200r/min, pH7.0, throughput 0.7vvm, after culture 36h, then is transferred to 500L hairs by 10v/v% inoculum concentrations
Fermentation tank, in 220r/min, pH7.0, under 0.7~1.0vvm of throughput, cultivates 3d.
3. the preparation of Alcaligenes faecalis Y1 microbial inoculums
Protective agent sodium alginate, Alcaligenes faecalis Y1 is added to be adsorbed with precipitated calcium carbonate after fermentation.
4. Alcaligenes faecalis Y1 microbial inoculums granulation
Appropriate powdered rice hulls, powder of straw and bentonite bonded pelleting are added in the microbial inoculum for adsorbed to the above, mixing, according to life
(viable count is 5 × 10 to the granular bacteria preparation of the product needs suitable sunflower of granulation formation8Cfu/g), with sunflower seeds seeder one
Rise and be manured into soil；Or form the granular biological fertilizer approximate with urea, Diammonium phosphate (DAP), potash fertilizer, composite fertilizer's particle diameter using comminutor
Material, its bio-feritlizer machinery together with urea, Diammonium phosphate (DAP), potash fertilizer, composite fertilizer is manured into soil.
Influence of the Alcaligenes faecalis Y1 microbial inoculums of embodiment 2 to potted plant sunflower growth-promoting effect
Growth-promoting effect of the 1.Y1 microbial inoculums to the potted plant sunflower of nonsaline-alkali soil earth
The inoculation test of Alcaligenes faecalis Y1 microbial inoculums is carried out under greenhouse experiment, sunflower has been planted under condition of pot, respectively
2 kinds of bio-feritlizer microbial inoculums of inoculation.Result of the test shows that microbe inoculation microbial inoculum can improve the biomass (table 1) of sunflower.
Y1 microbial inoculum biomass highests, 46.0% is improved than control (not applying microbial bacterial agent)；Inoculation Alcaligenes faecalis CC1d (this laboratory
Separating) the sunflower biomass of microbial inoculum is substantially less than Y1, still improves 30.1% than control.Result of the test show Y1 microbial inoculums promote to
Day certain herbaceous plants with big flowers growth result is significantly (table 1).
Influence of the bio-feritlizer of table 1 to the potted plant sunflower biomass of nonsaline-alkali soil
Note：A, b, c represent significant difference between treatment group.
Growth-promoting effect of the 2.Y1 microbial inoculums to the potted plant sunflower of slight saline-alkali soil
The inoculation test of Y1 microbial inoculums is carried out under greenhouse experiment, experiment soil comes from Inner Mongol Wu Lategu early stages, gently
Degree is saline and alkaline, and sunflower is planted year after year, and sclerotiniose and rust are abnormal serious, have no harvest within 2014.Sunflower connects respectively under condition of pot
2 kinds of bio-feritlizer microbial inoculums are planted, CCla bacterial strains are to be isolated from Ji Sheng Gongzhuling soil by this laboratory, are accredited as Alcaligenes faecalis
(Alcaligenes faecalis), carries out disease-resistant yield-increasing effect disquisition together with Y1.Result of the test shows, all microbial bacterias
Agent can improve the biomass of sunflower, and Y1 microbial inoculum biomass highests improve 21.57% than control；Alcaligenes faecalis CCla bacterium
The sunflower biomass of agent is slightly below Y1 microbial inoculums, but still improves 14.71% than control.Show that Y1 microbial inoculums are promoting sunflower life
Long side effect is significantly (table 2).
Influence of the bio-feritlizer to slight saline-alkali soil sunflower biomass under the condition of pot of table 2
Note：A, b represent significant difference between treatment group.
The action effect of the Y1 microbial inoculum Crops ' yield of embodiment 3
Under chemical fertilizer, a large amount of use conditions of organic fertilizer, the action effect to 4 kinds of bio-feritlizers is studied.N P and K
Composite fertilizer (15:15:15) consumption 83kg/ mus, 7t/ mus of organic fertilizer (chicken manure) consumption.(this laboratory separates bacterial strain J15, is accredited as
Stenotrophomonas maltophilia belongs to Stenotrophomonas sp.), JK24 (this laboratory separate, be accredited as bacillus megaterium
Bacillus megaterium), JK8 (this laboratory separate, be accredited as middle shortwave monad Brevundimonas
Intermedia) three bacterial strains are isolated from Gongzhuling of Jilin Province's agricultural land soil by this laboratory, and field is carried out together with bacterial strain Y1
Between test.Yield result shows that Crops ' is inoculated with 4 kinds of bio-feritlizers and significantly increases production, with the western red of bio-feritlizer Y1, J15
Persimmon obvious effect of increasing production, 866.4kg/ mus and 741.6kg/ mus is increased production than control respectively, is respectively increased 35.0% and 30.0%,
JK8 microbial inoculums only increase production 212.9kg/ mus and improve 8.6% than control.In all treatment, the effect of increasing production of Y1 microbial inoculums is preferably (table 3).
Influence (2nd results) of the different bio-feritlizer microbial inoculums of table 3 to tomato production
Note：A, b, c represent significant difference between treatment group.
Action effect of the Alcaligenes faecalis Y1 microbial inoculums of embodiment 4 to field sunflower
The Wulate Front Banner of Baya ur, NeiMengGu alliance carried out different microorganisms microbial inoculum preventing and treating sunflower control sclerotiniose,
The experimental study of handle rest fungus disease.Soil is saline-alkali soil, and Sunflower Varieties are LDS009, nitrogen-phosphorus-potassium compound fertilizer (15:15:15) use
Measure is 40kg/ mus.Jinxiang County of Shandong Province garlic soil (is isolated from by this laboratory with aspergillus niger C2 (CGMCC NO.7511), Wu5
Earth, is accredited as aspergillus niger) bacteria agent is prepared, bacteria agent consumption is 60kg/ mus.Result shows, throughout the growing season to day
Certain herbaceous plants with big flowers does not have any disease, has not both had sclerotium fungus diseases, does not have a handle rest fungus disease yet, and surrounding farmland sunflower sclerotium
Disease is serious caused by bacterium, handle rest fungus, most farmland total crop failures.Field experiment yield result shows, the sunflower of bacterial strain Y1
Yield is up to 216.1 kilograms, rate of growth 16.8%；Aspergillus niger Wu5 yield is taken second place, 209.2 kilograms of per mu yield, rate of growth
13%；Aspergillus niger C2 rate of growths are slightly below bacterial strain Wu5, and per mu yield reaches 205.7 kilograms, volume increase 11.1%.Show that Y1 has good
Good effect of increasing production and application potential (table 4).
The different bio-feritlizer microbial inoculums of table 4 are to sunflower yield effect
Note：A, b represent significant difference between treatment group.
Production-increasing function of the Y1 microbial inoculums of embodiment 5 to field sunflower
Field test mainly studies the best fit effect of bio-feritlizer and conventional Diammonium phosphate (DAP), gives full play to bio-feritlizer
With the maximum effect of chemical fertilizer.(this laboratory is isolated from Inner Mongol crow to put 2 (ACCC40120, streptomyces griseuses), QQ6 using bacterial strain
Draw it is special before flag saline-alkali soil, be accredited as aspergillus niger), Y1 bacterial strains prepare 3 kinds of bio-feritlizers.Bio-feritlizer sets 4 levels, be respectively 0,
50th, 100 and 150kg/ mus；40kg/ mus of Diammonium phosphate (DAP) consumption, all treatment are in triplicate.Result shows, all fertilizer amount bars
Under part, the yield (table 5) of sunflower can be improved using 4 levels of Alcaligenes faecalis Y1 bio-feritlizers.Use Diammonium phosphate (DAP)
Under the conditions of 40kg/ mus, three bio-feritlizer consumptions all show obvious effect of increasing production, as bio-feritlizer consumption increases, to
Day certain herbaceous plants with big flowers yield continuous improvement.50kg/ mu of bio-feritlizer of inoculation, bacterial strain put 2, QQ6 and Y1 increase production 12.7% respectively than control,
27.5% and 29.1%；100kg/ mu of bio-feritlizer of inoculation, bacterial strain put 2, QQ6 and Y1 increase production 26.8% respectively than control,
27.0% and 31.0%；Inoculation bio-feritlizer maximum, reaches 150kg/ mus, and bacterial strain is put 2, QQ6 and Y1 and increased production respectively than control
28.3%th, 32.0% and 32.8%.When being inoculated with 50kg/ mus, sunflower yield with 100kg/ mus of difference less, uses di(2-ethylhexyl)phosphate
Ammonium 40kg/ mus coordinates 50kg/ mus of bio-feritlizer proper (table 5).
Influence of the Alcaligenes faecalis Y1 bio-feritlizers of table 5 to field sunflower yield
Note：A, b, c represent significant difference between treatment group.
The Y1 bacterial strain flat boards of embodiment 6 suppress the effect of pinch outs
In order to verify the ability of Y1 antagonizing pathogenic fungis, sharp fusarium disease fungus (ACCC36241) is seeded in PDYA trainings
Base flat board center is supported, it is 4 points to be evenly dividing around pathogen, wherein 3 point inoculation Y1 bacterium, are not inoculated with Y1 separately, made
It is control.Result shows that 3 pathogens for being inoculated with Y1 are suppressed, and the pinch outs colony growth without being inoculated with Y1 is very fast.
Prove that Y1 has the ability for suppressing thread disease fungus growth.(Fig. 1)
The present invention to antagonism sclerotium blight of sunflower and rust, the efficient Antagonistic Fungi Y1 of preventing and treating booth vegetable continuous cropping obstacle and its
Microbial bacterial agent has carried out many-sided research, and the microbial inoculum can prevent and treat sclerotium blight of sunflower and rust, preventing and treating booth vegetable continuous cropping
Obstacle, improves crop yield.The Y1 microbial bacterial agents of the disease-resistant yield-increasing of exploitation, show huge application prospect.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Sequence table
<110>INST OF AGRICULTURAL RESOURCES
<120>Alcaligenes faecalis Y1 and its application in soil-borne disease of crop is prevented and treated
<130> KHP161119321.4Q
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1051
<212> DNA
<213>Alcaligenes faecalis (Alcaligenes faecalis)
<400> 1
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actactcgaa agagtggcta ataccgcata cgccctacgg gggaaagggg gggatcgcaa 180
gacctctcac tattggagcg gccgatatcg gattagctag ttggtggggt aaaggctcac 240
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gcccagactc ctacgggagg cagcagtggg gaattttgga caatggggga aaccctgatc 360
cagccatccc gcgtgtatga tgaaggcctt cgggttgtaa agtacttttg gcagagaaga 420
aaaggtatct cctaatacga gatactgctg acggtatctg cagaataagc accggctaac 480
tacgtgccag cagccgcggt aatacgtagg gtgcaagcgt taatcggaat tactgggcgt 540
aaagcgtgtg taggcggttc ggaaagaaag atgtgaaatc ccagggctca accttggaac 600
tgcattttta actgccgagc tagagtatgt cagagggggg tagaattcca cgtgtagcag 660
tgaaatgcgt agatatgtgg aggaataccg atggcgaagg cagccccctg ggataatact 720
gacgctcaga cacgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780
ctaaacgatg tcaactagct gttggggccg ttaggcctta gtagcgcagc taacgcgtga 840
agttgaccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg acggggaccc 900
gcacaagcgg tggatgatgt ggattaattc gatgcaacgc gaaaaacctt acctaccctt 960
gacatgtctg gaaagccgaa gagatttggc cgtgctcgca agagaaccgg aacacaggtg 1020
ctgcatggct gtcgtcagct cgtgtcgtga a 1051
Claims (10)
1. Alcaligenes faecalis (Alcaligenes faecalis) Y1, preserving number CGMCC NO.13510.
2. the microbial bacterial agent of Alcaligenes faecalis Y1 described in claim 1 is contained.
3. microbial inoculum according to claim 2, it is characterised in that also contain additive and protective agent in the microbial inoculum；
Wherein, the additive is in silica, precipitated calcium carbonate, kaolin, bentonite, powdered rice hulls, wheat husk powder, powder of straw
At least one；
The protective agent is at least one in glycerine, defatted milk, vegetable oil, sodium alginate, shitosan.
4. microbial inoculum according to claim 3, it is characterised in that be made up of the component of following weight portion：
1 part of Alcaligenes faecalis Y1 zymotic fluids；
Additive 0.5-3 parts；
Protective agent 0.01-0.05 parts；
Alcaligenes faecalis Y1 viable counts are 2 × 10 in the microbial inoculum8-5×109cfu/g。
5. Alcaligenes faecalis Y1 described in claim 1 or microbial inoculum described in claim any one of 2-4 are in soil-borne disease of crop is prevented and treated
Application.
6. the application described in claim 5, it is characterised in that the pathogen of the soil-borne disease includes sclerotinite, sunflower handle
Rest fungus, pinch outs.
7. Alcaligenes faecalis Y1 described in claim 1 or microbial inoculum described in claim any one of 2-4 in crop yield is improved should
With.
8. application according to claim 7, it is characterised in that the crop includes booth vegetable；Preferably, the crop
Including sunflower, garlic, muskmelon, tomato.
9. Alcaligenes faecalis Y1 described in claim 1 or microbial inoculum described in claim any one of 2-4 are in agricultural Chemical Mixed Fertilizer is prepared
Using.
10. a kind of agricultural Chemical Mixed Fertilizer, it is characterised in that microbial inoculum and nitrogenous fertilizer, phosphate fertilizer, potash fertilizer as described in claim any one of 2-4
Or composite fertilizer's composition.
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Cited By (4)
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| CN107488608A (en) * | 2017-06-08 | 2017-12-19 | 北京理工大学 | One plant of Streptomycesalbidoflhaving Z9 and its application in preventing and treating sclerotium blight of sunflower |
| CN109022311A (en) * | 2018-07-27 | 2018-12-18 | 华南农业大学 | One plant of Alcaligenes faecalis BC13 and its application in prevention and treatment rop bacterium venereal disease evil |
| CN109089730A (en) * | 2018-09-07 | 2018-12-28 | 景洪宏臻农业科技有限公司 | A kind of cultivation Boletus aereus earthing method |
| CN116240148A (en) * | 2023-04-04 | 2023-06-09 | 云南省农业科学院农业环境资源研究所 | Alcaligenes faecalis SZ-220101 and application thereof in preventing and controlling plant diseases |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107488608A (en) * | 2017-06-08 | 2017-12-19 | 北京理工大学 | One plant of Streptomycesalbidoflhaving Z9 and its application in preventing and treating sclerotium blight of sunflower |
| CN107488608B (en) * | 2017-06-08 | 2020-07-10 | 北京理工大学 | Streptomyces albidoflavus Z9 and application thereof in preventing and treating sunflower sclerotiniose |
| CN109022311A (en) * | 2018-07-27 | 2018-12-18 | 华南农业大学 | One plant of Alcaligenes faecalis BC13 and its application in prevention and treatment rop bacterium venereal disease evil |
| CN109022311B (en) * | 2018-07-27 | 2020-12-11 | 华南农业大学 | Alcaligenes faecalis BC13 and application thereof in prevention and treatment of crop bacterial diseases |
| CN109089730A (en) * | 2018-09-07 | 2018-12-28 | 景洪宏臻农业科技有限公司 | A kind of cultivation Boletus aereus earthing method |
| CN116240148A (en) * | 2023-04-04 | 2023-06-09 | 云南省农业科学院农业环境资源研究所 | Alcaligenes faecalis SZ-220101 and application thereof in preventing and controlling plant diseases |
| CN116240148B (en) * | 2023-04-04 | 2023-09-08 | 云南省农业科学院农业环境资源研究所 | Alcaligenes faecalis SZ-220101 and application thereof in preventing and controlling plant diseases |
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|---|---|
| CN106801024B (en) | 2020-03-27 |
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