CN109734484A - A kind of microorganism formulation and preparation method thereof conducive to salt-soda soil plant growth - Google Patents
A kind of microorganism formulation and preparation method thereof conducive to salt-soda soil plant growth Download PDFInfo
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Abstract
The invention belongs to microorganism formulation technical fields, are related to a kind of microorganism formulation and preparation method thereof conducive to salt-soda soil plant growth；Microorganism formulation includes 60-70 parts of starch, 8-10 parts of microbial bacterial agent, 20-32 parts of algal polysaccharides；Microbial bacterial agent is the brevibacterium (Brevibacterium sp.TS22) tamed by low altitude area and room temperature, deposit number are as follows: CGMCC 16576；Microbial bacterial agent may also include the mix bacterium agent of Halophiles and bacillus, and weight is the 0~20% of microbial bacterial agent；The preparation step of microorganism formulation is as follows: the starting components of microorganism formulation being put into blender according to parts by weight, are stirred to react, the microorganism formulation of plant root growth is promoted；Microorganism formulation of the invention is used alone or agroforestry organic fertilizer uses；Operation of the present invention is easy, and production cost is low, can effectively facilitate the plant growth in salt-soda soil.
Description
Technical field
The invention belongs to plant growth microorganism formulation technical fields, and in particular to one kind is conducive to salt-soda soil plant growth benefit
In the microorganism formulation and preparation method thereof of salt-soda soil plant growth.
Background technique
Saline-alkali soil is the civil general designation to solonchak and alkaline earth.Soil salt content is in 0.1%-0.2% or more or soil
Colloid adsorbs a certain number of exchangeable sodiums, and basicity generates serious shadow in 15%-20% or more, to the normal growth of crop
It rings, such penus is also known as salinized soil in saline-alkali soil, saline-alkali soil.According to incompletely statistics, about 9.5 hundred million hectares of the whole world is saline and alkaline
Ground；The salt-soda soil of China is mainly distributed on the inland arid of North China, northeast and northwest, semiarid zone, the strand of East Coastal
Area is also distributed.
Salt stress is one of the abiotic component the most serious for influencing plant growth；One side salinization of soil can reduce crop
Germination percentage；On the other hand, salt ionic concentration is higher in salinization of soil soil, and the salt ion of high concentration easily causes under soil water potential
Drop, therefore water stress is generated to plant, while also generating ionic stress, destroy intracellular ionic equilibrium；Salinity is also
It will lead to the accumulation of active oxygen in plant, to injure cell or even kill it；In addition, grown in salinization soil
Plant often lacks Ca2+And K+, so that plant is generated Nutrient Stress, the growth and development of such plant will be abnormal.Salt stress pair
The overall performance of plant growth and development is to inhibit the growth of tissue and organ, and accelerated development process shortens nutrient growth and reproduction
Phase.
So being attempted to develop and utilize saline and alkaline soil from the directions such as agricultural, chemistry, biology.According to ameliorative measure
Difference, different effects can be obtained for the development and utilization in salt-soda soil.Improvement solonchak can be arranged by draining, desalinization of soil by flooding or leaching etc.
It applies, or its salt is improved with crops such as green manuring, organic fertilizer or kind rice；Improvement of these methods to saline-alkali soil
Although there is certain effect, effect is unstable, and in practical application, the throwing of a large amount of human and material resources and financial resources
Enter virtually to be significantly greatly increased the cost of this measure.
Summary of the invention
In view of the above-mentioned problems, present invention seek to address that one of described problem, provides a kind of conducive to salt-soda soil plant growth benefit
In the microorganism formulation and preparation method thereof of salt-soda soil plant growth, specific technical solution is as follows:
Present invention firstly provides a kind of microorganism formulation for being conducive to salt-soda soil plant growth conducive to salt-soda soil plant growth, packets
Include following starting components and parts by weight, 60-70 parts of starch, 8-10 parts of microbial bacterial agent, 20-32 parts of algal polysaccharides.
The microbial bacterial agent is the brevibacterium (Brevibacterium sp.TS22) derived from Qinghai-Tibet Platean through too low sea
It pulls out and tames and obtain with room temperature, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows:
CGMCC 16576。
The mode of the low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture
In base, in secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained.
The LB culture medium includes tryptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, pH 7.4.
The microbial bacterial agent further includes Halophiles and the mix bacterium agent that bacillus is prepared；In mix bacterium agent Halophiles and
The number of strains ratio of bacillus is 1:1.
The bacillus is gel-shaped series bacillus, bacillus subtilis or bacillus licheniformis；
Halophiles needs to tame culture, specific steps are as follows: Halophiles is inoculated into TSA culture medium and adds 8%-10%
(w/v) NaCl carries out continuous passage culture at room temperature；The TSA culture medium preparation method are as follows: soy peptone 3g/L,
Tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L, 115 DEG C of high steams with deionized water
Sterilize 20min.
The domestication and culture method of the bacillus are as follows: bacillus is inoculated into LB culture medium, under room temperature
Secondary culture repeatedly.
Halophiles and bacillus exist after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
In use, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will pass through
The brevibacterium of low altitude area and room temperature domestication, Halophiles and the bacillus after domestication culture after domestication culture, respectively
It is inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L,
Yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
The weight of the mix bacterium agent is the 0-20% of microbial bacterial agent.
The present invention also provides a kind of preparation methods of microorganism formulation conducive to salt-soda soil plant growth, and specific steps are such as
Under:
The starting components of microorganism formulation are put into blender according to parts by weight, the revolving speed that blender is arranged exists
Room temperature item is reacted, and the microorganism formulation conducive to salt-soda soil plant growth is obtained.
The revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min
The microorganism formulation can also be used in mixed way with agricultural organic fertilizer.
Beneficial effects of the present invention:
(1) microorganism formulation prepared by the present invention can effectively be conducive to salt-soda soil plant growth and be conducive to salt-soda soil plant growth；
After adding microorganism formulation processing, the root long of plant can be improved 30% or more compared to control group, and the root of plant is with heavy phase
Than can be improved 60% or so in control group, the fibrous root number of plant improves 75% or so, this explanation is in formula of the invention
The growth for remarkably promoting salt-soda soil plant can be reached down；In addition, microorganism formulation prepared by the present invention can also have with agricultural
Machine fertilizer is used in combination, and can preferably improve the growth of plant.
(2) operation of the present invention is easy, and production cost is low, has highly important theory and practice meaning.
Specific embodiment
In the present invention, the deposit number of Halophiles is CICC23817, purchased from the management of Chinese industrial Microbiological Culture Collection
The heart；Gel-shaped series bacillus deposit number is BNCC19068, bacillus subtilis deposit number is ATCC6633, lichens bud
Spore bacillus deposit number is BNCC221075, is purchased from BeNa Culture Collection Institute of Biotechnology.
Brevibacterium Brevibacterium sp.TS22, it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Deposit number are as follows: CGMCC 16576；Preservation date: 2018
On October 12, in.
Brevibacterium sp.TS22 sequence information:
>CCCCGCCATCGGCTTCGTTCGAATGCCATGCATAGCGACTCGCACGCCAAGCGTG CGTCCCTGAGC
CATGATCAAACTCTATGAGTAACACGTGGGCAACCTGCCTATAAGACT GGGATAACTTCGGGAAACCGGAGCTAA
TACCGGATACGTTCTTTTCTCGCATGAGAGAA GATGGAAAGACGGTTTACGCTGTCACTTATAGATGGGCCCGCG
GCGCATTAGCTAGTTG GTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCC
ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCC GCAATGGACGAAAGTCT
GACGGAGCAACGCCGCGTGAACGAAGAAGGCCTTCGGGTC GTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCAG
AGTAACTGCTGGTACCTTGACGGT ACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACG
TAGGTGGC AAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTCCTTAAGTCTGATG TGAAAGC
CCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGA AGAGGAAAGTGGAATTCCAAAGTG
TAGCGGTGAAATGCGTAGAGATTTGGAGGAACAC CAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAG
GCGCGAAAGCGTGGGGA GCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAG
AGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACG GCCGCAAGGCTGAAAC
TCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACC
AGGTCTTGACATCCTCTGACAACCCT AGAGATAGGGCTTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGG
TTGTCGTCAG CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTG CCAGC
ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGG GATGACGTCAAATCATCATGCC
CCTTATGACCTGGGCTACACACGTGCTACAATGGATGG TACAAAGGGCTGCAAACCTGCGAAGGTAAGCGAATCC
CATAAAGCCATTCTCAGTTCGG ATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGAT
CAGCAT GCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC
Embodiment 1:
A kind of microorganism formulation conducive to salt-soda soil plant growth, including following starting components and parts by weight, starch
70 parts, 10 parts of microbial bacterial agent, 20 parts of algal polysaccharides, microbial bacterial agent further include the mixed of Halophiles and gel-shaped series bacillus
Combined bacteria agent, total weight are the 10% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
Need to tame culture before Halophiles use；The domestication culture is to be inoculated into Halophiles in TSA culture medium to add
Add the NaCl of 8%-10% (w/v), carries out continuous passage culture at room temperature.The TSA culture medium preparation method are as follows: soybean
Peptone 3g/L, tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L with deionized water,
115 DEG C of high pressure steam sterilization 20min.
The acclimation method again of the gel-shaped series bacillus is: bacillus being inoculated into LB culture medium, in room temperature
Under the conditions of secondary culture repeatedly.
Halophiles and gel-shaped class bud after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
Spore bacillus when in use, need to pass through taming again for condition corresponding with that will use area's main environmental conditions, then the condition tamed
Are as follows: it will be by the brevibacterium of low altitude area and room temperature domestication, Halophiles and the jelly after domestication culture after domestication culture
Sample series bacillus is inoculated into respectively in LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is such as
Under: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high steams with NaOH
Sterilize 20min.
A kind of preparation method of the microorganism formulation conducive to salt-soda soil plant growth, the specific steps are as follows:
The starting components of microorganism formulation are put into blender according to parts by weight to mix, the revolving speed of blender
It is set as 180r/min, temperature is room temperature, and the microorganism system conducive to salt-soda soil plant growth is made in reaction time 22min
Agent.Microorganism formulation is that 1:99 is uniformly mixed with agricultural organic fertilizer by weight when application.
Embodiment 2:
A kind of microorganism formulation conducive to salt-soda soil plant growth, including following starting components and parts by weight, starch
60 parts, 8 parts of microbial bacterial agent, 32 parts of algal polysaccharides, microbial bacterial agent further includes the Mixed Microbes of Halophiles and bacillus licheniformis
Agent, total weight are the 20% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
Need to tame culture before Halophiles use；The domestication culture is to be inoculated into Halophiles in TSA culture medium to add
Add the NaCl of 8%-10% (w/v), carries out continuous passage culture at room temperature.The TSA culture medium preparation method are as follows: soybean
Peptone 3g/L, tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L with deionized water,
115 DEG C of high pressure steam sterilization 20min.
Wherein the acclimation method again of bacillus licheniformis is: bacillus licheniformis being inoculated into LB culture medium, in room temperature
Under the conditions of secondary culture repeatedly, obtain the bacillus licheniformis for adapting to low altitude area and normal temperature condition.
Halophiles and bacillus licheniformis exist after the brevibacterium and domestication culture of low altitude area and room temperature domestication acquisition
In use, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will pass through
The brevibacterium of low altitude area and room temperature domestication, Halophiles and the bacillus licheniformis after domestication culture after domestication culture,
It is inoculated into LB culture medium respectively, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone
10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method of the microorganism formulation conducive to salt-soda soil plant growth, the specific steps are as follows:
The starting components of microorganism formulation are put into blender according to parts by weight to mix, the revolving speed of blender
It is set as 200r/min, temperature is room temperature, and the microorganism system conducive to salt-soda soil plant growth is made in reaction time 20min
Agent.Microorganism formulation is that 2:98 is uniformly mixed with agricultural organic fertilizer by weight when application.
Embodiment 3:
A kind of microorganism formulation conducive to salt-soda soil plant growth, including following starting components and parts by weight, starch
68 parts, 8 parts of microbial bacterial agent, 24 parts of algal polysaccharides, the mix bacterium agent of microbial bacterial agent Halophiles and gel-shaped series bacillus,
Total weight is the 20% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
Need to tame culture before Halophiles use；The domestication culture is to be inoculated into Halophiles in TSA culture medium to add
Add the NaCl of 8%-10% (w/v), carries out continuous passage culture at room temperature.The TSA culture medium preparation method are as follows: soybean
Peptone 3g/L, tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L with deionized water,
115 DEG C of high pressure steam sterilization 20min.
The acclimation method again of the gel-shaped series bacillus is: gel-shaped series bacillus is inoculated into LB culture medium
In, in secondary culture repeatedly under room temperature, obtain the gel-shaped series bacillus for adapting to low altitude area and normal temperature condition.
Halophiles and gel-shaped class bud after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
Spore bacillus when in use, need to pass through taming again for condition corresponding with that will use area's main environmental conditions, then the condition tamed
Are as follows: it will be by the brevibacterium of low altitude area and room temperature domestication, Halophiles and the jelly after domestication culture after domestication culture
Sample series bacillus is inoculated into respectively in LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is such as
Under: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high steams with NaOH
Sterilize 20min.
A kind of preparation method of the microorganism formulation conducive to salt-soda soil plant growth, the specific steps are as follows:
The starting components of microorganism formulation are put into blender according to parts by weight to mix, the revolving speed of blender
It is set as 100r/min, temperature is room temperature, and the microorganism system conducive to salt-soda soil plant growth is made in reaction time 30min
Agent.
Embodiment 4:
A kind of microorganism formulation conducive to salt-soda soil plant growth, including following starting components and parts by weight, starch
65 parts, 5 parts of microbial bacterial agent, 30 parts of algal polysaccharides, microbial bacterial agent further include domestication culture after, equal proportion mixing Halophiles
With bacillus subtilis, total weight is the 10% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
Need to tame culture before Halophiles use；The domestication culture is to be inoculated into Halophiles in TSA culture medium to add
Add the NaCl of 8%-10% (w/v), carries out continuous passage culture at room temperature.The TSA culture medium preparation method are as follows: soybean
Peptone 3g/L, tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L with deionized water,
115 DEG C of high pressure steam sterilization 20min.
The acclimation method again of the bacillus subtilis is: gel-shaped series bacillus is inoculated into LB culture medium, in
Secondary culture repeatedly under room temperature obtains the bacillus subtilis for adapting to low altitude area and normal temperature condition.
Halophiles and bacillus subtilis after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
Bacterium when in use, need to pass through taming again for condition corresponding with that will use area's main environmental conditions, then the condition tamed are as follows: will
By the brevibacterium of low altitude area and room temperature domestication, Halophiles and the bacillus subtilis after domestication culture after domestication culture
Bacterium is inoculated into respectively in LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone
10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method for the microorganism formulation promoting salt-soda soil, the specific steps are as follows:
The starting components of microorganism formulation are put into blender according to parts by weight to mix, the revolving speed of blender
It is set as 250r/min, temperature is room temperature, and the microorganism system conducive to salt-soda soil plant growth is made in reaction time 15min
Agent.
Embodiment 5:
A kind of microorganism formulation conducive to salt-soda soil plant growth, including following starting components and parts by weight, starch
63 parts, 9 parts of microbial bacterial agent, 28 parts of algal polysaccharides, the microbial bacterial agent are the brevibacterium derived from Qinghai-Tibet Platean
(Brevibacterium sp.TS22) is obtained by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation management
Committee's common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: blueness will be isolated from
The brevibacterium of hiding plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtain adapting to low altitude area and
The brevibacterium of normal temperature condition；
The brevibacterium obtained by low altitude area and room temperature domestication when in use, need to pass through and will use the main ring in area
Condition corresponding condition in border is tamed again, then the condition tamed are as follows: is inoculated into the brevibacterium tamed by low altitude area and room temperature
In LB culture medium, the secondary culture repeatedly at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast mention
Object 5g/L, sodium chloride 10g/L are taken, adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method of the microorganism formulation conducive to salt-soda soil plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 280r/min, and temperature is room temperature, reaction
Time is 12min, and the microorganism formulation conducive to salt-soda soil plant growth is made.
Embodiment effect:
Field trial is to fertilizer functional verification:
Summer in choosing consistent salt-soda soil experimental plot in Gansu Lanzhou, is divided into 18 1 meter × 1 meter blocks, presses
Above-mentioned 5 embodiments and each three design iterations of blank control, the experiment each embodiment in plot use identical deal respectively
(i.e. 100 grams) are uniformly blended into soil, sow each 100, green vegetables seed, normal watering Irrigation counts emergence rate, then 10 after 10 days
Thinning after it respectively stays 50 plants；Harvest randomly chooses each 20 plants of titles gross weight after 30 days again.Control group does not add microorganism formulation and agriculture
The processing of woods organic fertilizer, remaining step are identical.Statistical result is described in table 1 below.Statistical result is described in table 1 below.
As can be seen that the effect of embodiment 1-5 is substantially better than the effect of control group from above-mentioned table 1；Individually add micro- life
After the processing of object preparation, the root long of plant can be improved 30% or more compared to control group, and the root of plant is with weight compared to control
Group can be improved 60% or so, and the fibrous root number of plant improves 75% or so, this explanation can reach under formula of the invention
To the growth for remarkably promoting salt-soda soil plant.In addition, from 1 result of table it has also been discovered that be properly added agricultural organic fertilizer can be obvious
Plant roots growth-promoting effect is improved, the root long of plant and can be improved 75% or more compared to control group with weight, the fibrous root number of plant
It is more than doubled.
Illustrate: above embodiments are only to illustrate the present invention and not limit the technical scheme described by the invention；Therefore,
Although this specification is referring to above-mentioned each embodiment, the present invention has been described in detail, the common skill of this field
Art personnel should be appreciated that and still can modify to the present invention or equivalent replacement；And all do not depart from spirit of the invention and
The technical solution and its improvement of range, should all cover in scope of the presently claimed invention.
Sequence table
<110>Jiangsu University
<120>a kind of microorganism formulation and preparation method thereof conducive to salt-soda soil plant growth
<160> 1
<170> SIPOSequenceListing 1.0
<210> 2
<211> 1384
<212> DNA
<213>brevibacterium (Brevibacterium sp.)
<400> 2
ccccgccatc ggcttcgttc gaatgccatg catagcgact cgcacgccaa gcgtgcgtcc 60
ctgagccatg atcaaactct atgagtaaca cgtgggcaac ctgcctataa gactgggata 120
acttcgggaa accggagcta ataccggata cgttcttttc tcgcatgaga gaagatggaa 180
agacggttta cgctgtcact tatagatggg cccgcggcgc attagctagt tggtgaggta 240
atggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac 300
tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa 360
agtctgacgg agcaacgccg cgtgaacgaa gaaggccttc gggtcgtaaa gttctgttgt 420
tagggaagaa caagtaccag agtaactgct ggtaccttga cggtacctaa ccagaaagcc 480
acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggaatt 540
attgggcgta aagcgcgcgc aggtggttcc ttaagtctga tgtgaaagcc cacggctcaa 600
ccgtggaggg tcattggaaa ctggggaact tgagtgcaga agaggaaagt ggaattccaa 660
agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg cgactttctg 720
gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 780
agtccacgcc gtaaacgatg agtgctaagt gttagagggt ttccgccctt tagtgctgca 840
gctaacgcat taagcactcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960
ttaccaggtc ttgacatcct ctgacaaccc tagagatagg gctttcccct tcgggggaca 1020
gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080
gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140
gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200
gggctacaca cgtgctacaa tggatggtac aaagggctgc aaacctgcga aggtaagcga 1260
atcccataaa gccattctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1320
atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380
cgcc 1384
Claims (10)
1. a kind of microorganism formulation conducive to salt-soda soil plant growth, which is characterized in that including following starting components and weight
Number, 60-70 parts of starch, 8-10 parts of microbial bacterial agent, 20-32 parts of algal polysaccharides；The microbial bacterial agent is derived from Qinghai-Tibet height
Former brevibacterium Brevibacterium sp.TS22 tames by low altitude area and room temperature and is obtained, is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, deposit number are as follows: CGMCC 16576.
2. a kind of microorganism formulation conducive to salt-soda soil plant growth according to claim 1, which is characterized in that described low
The condition of height above sea level and room temperature domestication are as follows: brevibacterium Brevibacterium sp.TS22 is inoculated into LB culture medium, in room temperature
Under the conditions of secondary culture repeatedly；The LB culture medium includes tryptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, pH
It is 7.4.
3. a kind of microorganism formulation conducive to salt-soda soil plant growth according to claim 1, which is characterized in that described micro-
Bacteria agent further includes Halophiles and the mix bacterium agent that bacillus is prepared, the bacterial strain of Halophiles and bacillus in mix bacterium agent
Number ratio is 1:1；The weight of the mix bacterium agent is the 0-20% of microbial bacterial agent.
4. a kind of microorganism formulation conducive to salt-soda soil plant growth according to claim 3, which is characterized in that described thermophilic
Salt bacterium needs to tame culture before use；The domestication culture is to be inoculated into Halophiles in TSA culture medium to add mass concentration
For the NaCl of 8%-10%, continuous passage culture is carried out at room temperature；The TSA culture medium preparation method are as follows: soy peptone
3g/L, tryptone 17g/L, dipotassium hydrogen phosphate 2.5g/L, glucose 2.5g/L are settled to 1L, 115 DEG C of high pressures with deionized water
Steam sterilizing 20min；The bacillus needs training culture before use, and the domestication culture is to be inoculated into bacillus
In LB culture medium, in secondary culture repeatedly under room temperature；The LB culture medium includes tryptone 10g/L, yeast extract
5g/L, sodium chloride 5g/L, pH 7.4.
5. a kind of microorganism formulation conducive to salt-soda soil plant growth according to claim 3, which is characterized in that the bud
Spore bacillus is gel-shaped series bacillus, bacillus subtilis or bacillus licheniformis.
6. a kind of microorganism formulation conducive to salt-soda soil plant growth according to claim 2 or 4, which is characterized in that institute
State after brevibacterium that low altitude area and room temperature domestication obtain and domestication culture Halophiles and bacillus when in use, need through
Cross taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed are as follows: will by low altitude area and often
The brevibacterium of docilityization, Halophiles and the bacillus after domestication culture after domestication culture, are inoculated into LB training respectively
It supports in base, secondary culture is carried out at 20-25 DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract
5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
7. a kind of preparation method of microorganism formulation conducive to salt-soda soil plant growth according to claim 1, feature
It is, the specific steps are as follows: put into the starting components of microorganism formulation in blender according to parts by weight, setting stirring
The revolving speed of machine is reacted in room temperature item, obtains the microorganism formulation conducive to salt-soda soil plant growth.
8. a kind of preparation method of microorganism formulation conducive to salt-soda soil plant growth according to claim 7, feature
It is, the revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min.
9. being used for a kind of microorganism formulation conducive to salt-soda soil plant growth described in claim 1 to be conducive to salt-soda soil plant raw
Long purposes, which is characterized in that the microorganism formulation conducive to salt-soda soil plant growth is used alone or agroforestry is organic
Fertilizer uses.
10. purposes according to claim 9, which is characterized in that the microorganism formulation conducive to salt-soda soil plant growth
Agroforestry organic fertilizer is in use, being conducive to the microorganism formulation of salt-soda soil plant growth and the mass ratio of agricultural organic fertilizer is 1-2:
98-99。
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