CN106754463A - One plant of tool dissolving P capacity Burkholderia bacterium NJAU B8 and its microbial manure of development - Google Patents
One plant of tool dissolving P capacity Burkholderia bacterium NJAU B8 and its microbial manure of development Download PDFInfo
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- CN106754463A CN106754463A CN201611001344.XA CN201611001344A CN106754463A CN 106754463 A CN106754463 A CN 106754463A CN 201611001344 A CN201611001344 A CN 201611001344A CN 106754463 A CN106754463 A CN 106754463A
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- 241001453380 Burkholderia Species 0.000 title claims abstract description 16
- 230000000813 microbial Effects 0.000 title claims abstract description 14
- 210000003608 Feces Anatomy 0.000 title claims abstract description 11
- 239000010871 livestock manure Substances 0.000 title claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 56
- 241000894006 Bacteria Species 0.000 claims abstract description 47
- 230000001580 bacterial Effects 0.000 claims abstract description 33
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 25
- 239000002689 soil Substances 0.000 claims abstract description 23
- 235000005822 corn Nutrition 0.000 claims abstract description 21
- 235000005824 corn Nutrition 0.000 claims abstract description 21
- 230000012010 growth Effects 0.000 claims abstract description 19
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 14
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims abstract description 11
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims abstract description 11
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims abstract description 11
- UKNAYQWNMMGCNX-UHFFFAOYSA-N sodium;[hydroxy(phenyl)methyl]-oxido-oxophosphanium Chemical compound [Na+].[O-][P+](=O)C(O)C1=CC=CC=C1 UKNAYQWNMMGCNX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000005602 Coleus parviflorus Nutrition 0.000 claims abstract description 7
- 240000002347 Colocasia esculenta Species 0.000 claims abstract description 7
- 235000002722 Dioscorea batatas Nutrition 0.000 claims abstract description 7
- 241000209149 Zea Species 0.000 claims abstract 6
- 239000003337 fertilizer Substances 0.000 claims description 65
- 235000015097 nutrients Nutrition 0.000 claims description 42
- 239000002131 composite material Substances 0.000 claims description 27
- 230000002906 microbiologic Effects 0.000 claims description 25
- OAICVXFJPJFONN-UHFFFAOYSA-N phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 23
- 239000011574 phosphorus Substances 0.000 claims description 23
- 229910052698 phosphorus Inorganic materials 0.000 claims description 23
- 239000012530 fluid Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 13
- 230000035492 administration Effects 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- HNBDQABBWNOTRU-UHFFFAOYSA-N thalline Chemical compound C1=CC=[Tl]C=C1 HNBDQABBWNOTRU-UHFFFAOYSA-N 0.000 claims description 5
- 230000001737 promoting Effects 0.000 claims description 4
- 238000011049 filling Methods 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 238000009331 sowing Methods 0.000 claims 1
- 239000000375 suspending agent Substances 0.000 claims 1
- 229920001670 16S ribosomal RNA Polymers 0.000 abstract description 5
- 241001135516 Burkholderia gladioli Species 0.000 abstract description 3
- 238000002955 isolation Methods 0.000 abstract description 3
- 238000004450 types of analysis Methods 0.000 abstract description 3
- 244000150668 Zea mays subsp mays Species 0.000 abstract description 2
- 238000011002 quantification Methods 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 description 17
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 16
- 230000000694 effects Effects 0.000 description 15
- 229910010272 inorganic material Inorganic materials 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 229910000389 calcium phosphate Inorganic materials 0.000 description 8
- ZBZJARSYCHAEND-UHFFFAOYSA-L calcium;dihydrogen phosphate;hydrate Chemical compound O.[Ca+2].OP(O)([O-])=O.OP(O)([O-])=O ZBZJARSYCHAEND-UHFFFAOYSA-L 0.000 description 8
- 239000004202 carbamide Substances 0.000 description 8
- 235000019691 monocalcium phosphate Nutrition 0.000 description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 8
- 230000000875 corresponding Effects 0.000 description 7
- OTYBMLCTZGSZBG-UHFFFAOYSA-L Potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K [O-]P([O-])([O-])=O Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 229910052939 potassium sulfate Inorganic materials 0.000 description 6
- 235000011151 potassium sulphates Nutrition 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- BJHIKXHVCXFQLS-UYFOZJQFSA-N Fructose Natural products OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 4
- GUBGYTABKSRVRQ-YOLKTULGSA-N Maltose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)O[C@H]1CO)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 GUBGYTABKSRVRQ-YOLKTULGSA-N 0.000 description 4
- 240000001016 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 239000001103 potassium chloride Substances 0.000 description 4
- 235000011164 potassium chloride Nutrition 0.000 description 4
- 230000001954 sterilising Effects 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000013877 carbamide Nutrition 0.000 description 3
- 235000019804 chlorophyll Nutrition 0.000 description 3
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 3
- 229930002875 chlorophylls Natural products 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000005498 polishing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- -1 12.0%) Chemical compound 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 238000010159 Duncan test Methods 0.000 description 2
- GUBGYTABKSRVRQ-UUNJERMWSA-N Lactose Natural products O([C@@H]1[C@H](O)[C@H](O)[C@H](O)O[C@@H]1CO)[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1 GUBGYTABKSRVRQ-UUNJERMWSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L MgCl2 Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000589157 Rhizobiales Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H Tricalcium phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- SRBFZHDQGSBBOR-SQOUGZDYSA-N Xylose Natural products O[C@@H]1CO[C@@H](O)[C@@H](O)[C@@H]1O SRBFZHDQGSBBOR-SQOUGZDYSA-N 0.000 description 2
- 229960003487 Xylose Drugs 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 230000003213 activating Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 150000001479 arabinose derivatives Chemical class 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000003967 crop rotation Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000009499 grossing Methods 0.000 description 2
- 230000003301 hydrolyzing Effects 0.000 description 2
- 150000002484 inorganic compounds Chemical class 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 239000002609 media Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000877 morphologic Effects 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- 229920003227 poly(N-vinyl carbazole) Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000003381 solubilizing Effects 0.000 description 2
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 2
- 235000019731 tricalcium phosphate Nutrition 0.000 description 2
- 229940078499 tricalcium phosphate Drugs 0.000 description 2
- 241001508395 Burkholderia sp. Species 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- 230000037408 Distribution ratio Effects 0.000 description 1
- 241000602080 Dracaena fragrans Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H Iron(III) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000000560 biocompatible material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 229940032950 ferric sulfate Drugs 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000001963 growth media Substances 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000000474 nursing Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 239000002367 phosphate rock Substances 0.000 description 1
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses one plant of tool dissolving P capacity Burkholderia bacterium NJAU B8 and its microbial manure of development, the strain isolation is in field corn rhizosphere soil, it is accredited as Burkholder and belongs to bacterium based on physio-biochemical characteristics and 16S rDNA analyses, it reaches 99% with Burkholderia gladioli homology.Quantification and qualification result shows that bacterial strain NJAU B8 have compared with strong solution Phos ability；Potted plant to show with field experiment result, bio-bacterial manure and biological organic fertilizer based on the bacterial strain development are able to effectively facilitate the potted plant growth of corn and increase the crop field yield of corn, Chinese cabbage and potato.
Description
Technical field
The invention belongs to field of agricultural microorganism, and in particular to one plant of tool dissolving P capacity Burkholderia bacterium
NJAU-B8 and its microbial manure of development.
Background technology
Phosphorus is one of main nutrient elements of limiting plant growth, the phosphate fertilizer this season crop utilization rate for applying be 5%~
25%.Ca in most of phosphorus and soil2+、Fe3+、Fe2+、Al3+With reference to, formation insoluble phosphate (Abd-Alla M H,
1994), thus most of phosphate fertilizer accumulated in soil with the invalid state of indissoluble.Plant growth-promoting rhizobacteria (Plant Growth-
Promoting Rhizobacteria, PGPR) refer to and survive in plant rhizosphere, Gen Biao and can directly or indirectly promote and adjust
The microorganism (Ahmad et al, 2008) of plant growth, wherein phosphate solubilizing bacteria can be by insoluble phosphate, in ground phosphate rock
Phosphorus is converted into water-soluble condition, so as to utilize (Zhu Ying etc., 2009) for plant absorption.Such bacterium can slightly solubility in activating soil
Phosphorus element, can also increase root system of plant to around K, Ca, Mg, Fe, Zn by influenceing the type and quantity of secretions from plant roots
Deng the absorption of nutrient, the resistance of plant is improved, plant is grown under the stress such as acid, scarce phosphorus habitat.
Therefore, it is necessary to screen efficient phosphate-solubilizing bacterial strain from the plant rhizosphere under natural conditions, and it is made phosphate solubilizing microorganism and has
Machine fertilizer, with slightly solubility phosphorus element in activating soil while nutrition is provided, promotes absorption of the crops to phosphorus.
The content of the invention
It is an object of the invention to the practical problem and demand that are directed in production practices, there is provided one plant of tool phosphorus decomposing function, enter
And effectively facilitate plant growth and increase the Burkholderia bacterium NJAU-B8 of crop yield.
The composite microbiological fertilizer prepared it is a further object of the present invention to provide the bacterium and its application.
The microbial bacterial agent prepared it is yet another object of the invention to provide the bacterium and its application.
The purpose of the present invention can be achieved through the following technical solutions：
One plant is used to promoting the growth-promoting bacterial strain NJAU-B8 of plant growth, and strain isolation is from Tonghua, Jilin Province maize field rhizosphere
Soil, Classification And Nomenclature is Burkholderia bacterium (Burkholderia sp.), is preserved in Chinese microorganism strain preservation
Administration committee's common micro-organisms center, preservation date is on April 11st, 2016, and deposit number is CGMCC No.12339.
Bacterium colonies of the bacterial strain NJAU-B8 on LB flat boards is smaller, in faint yellow, and can produce xanthein, edge-smoothing, tool
It is a bit sticky, is difficult to provoke.Gram-negative, thalline uniform coloring.D-Fructose, maltose, arabinose, wood
Sugar, lactose utilization are positive, and glucose fermentation produces acid not aerogenesis, 7%NaCl growths.With the 16S rDNA of NJAU-B8 bacterial strains
The constructed development tree table of sequence (accession number is KX421207) is bright, bacterial strain NJAU-B8 and Burkholderia gladioli
Bukhdderia gladioli (EU024168) homology reaches 99.1%.Morphological feature, physicochemical characteristicses with reference to bacterial strain are simultaneously tied
16SrDNA sequence analyses are closed, bacterial strain NJAU-B8 is initially identified as Burkholderia bacterium.
Burkholder of the present invention belongs to bacterium NJAU-B8 answering in solution Phos or promotion plant growth and volume increase
With.
Burkholder of the present invention belongs to bacterium NJAU-B8 and is preparing the microbial bacterial agent of promotion plant yield-increasing or compound micro-
Application in bio-feritlizer.
Belong to the microbial bacterial agent and/or composite microbiological fertilizer of bacterium NJAU-B8 preparations by Burkholder of the present invention.
Described microbial bacterial agent preferably mainly passes to lower section method and prepares：It is the Bai Ke of CGMCC No.12339 by preserving number
Hall moral campylobacter bacteria NJAU-B8 is inoculated into LB fluid nutrient mediums, carries out liquid fermentation production, and the condition of fermenting and producing is：
PH 7.0,30~35 DEG C of fermentation temperature scope, rotating speed is 170r/min, and fermentation time is 48h, makes bacteria containing amount >=1 in zymotic fluid
×1010CFU/mL.LB cultures liquid making method used is, as a example by preparing 1L culture mediums：Peptone 10g, dusty yeast 5g, NaCl
10g, agar 20g, are settled to 1000mL, pH natures, 121 DEG C of sterilizing 20min.
The zymotic fluid that described microbial bacterial agent will further preferably be obtained conventionally is prepared as other microorganisms
Fertilizer common formulations, are preferably prepared as pulvis.
Application of the microbial bacterial agent of the present invention in solution Phos and promotion plant growth and volume increase.
Described promotion plant growth application, is preferably centrifuged 8~10min by described 6000~8000r/min of zymotic fluid
Afterwards, bacteria suspension is prepared into using physiological saline (concentration 0.9%) resuspended thalline equal with fermentating liquid volume, is then fed directly to
Plant rhizosphere.On the basis of reduces cost, the filling bacterium amount of bacteria suspension is 2.0~3.0 preferably by bacterium solution volume and soil quality ratio
×107CFU/g soil (butt) is applied, further preferably according to 2.5 × 107CFU/g soil (butt) is applied.
Described composite microbiological fertilizer preferably mainly passes to lower section method and prepares：By common organic fertilizer and amino acid hydrolyticsolution
By 5:1(w:V, kg：L) mix, by 20:1(w：V, kg：L NJAU-B8 zymotic fluids) are added, by secondary fermentation, obtaining biology has
Machine fertilizer, addition nutrient chemical to total nitrogen (N), full phosphorus (P2O5) and full potassium (K2O) nutrient is respectively 80gkg-1, 40gkg-1With
30g·kg-1Composite microbiological fertilizer is developed into, its living bacteria count >=0.5 hundred million/g fertilizer.Chemical fertilizer from urea (N,
46.6%), calcium superphosphate (P2O5, 12.0%), potassium chloride (K2O, 52.0%).
Described amino acid hydrolyticsolution is preferably prepared by the following method and forms：(1) will first die of illness poultry in closed container
After fowl animal is transferred to closed hydrolytic decomposition pot automatically through automation crushing, all solid contents and liquid, in initial acid c (1/
2H2SO4) it is 3-5mol L-1, hydrolyze 2-5 hours under 80-100 DEG C and 1-2 atmospheric pressure；(2) hydrolysis end treats molten in hydrolytic decomposition pot
When liquid is cooled to less than 80 DEG C, stratification, the Freamine Ⅲ for collecting middle level is described amino acid hydrolyticsolution.Amino acid
Hydrolyzate containing amino acid and various peptide matters about 10% (g/100ml) and more than.
Application of the composite microbiological fertilizer of the present invention in solution Phos and/or promotion plant growth and volume increase.
Described promotion crop yield application, field test continuously plant 3 season crop：Corn, Chinese cabbage and potato crop rotation.Often
Season, biological organic fertilizer applied 3000kghm-2, points of 2 times administrations of fertilizer plant before seedling and apply the 1/2 of total nutrient as base manure (bag
Include the chemical fertilizer of all of organic-inorganic compound mixed fertilizer or composite microbiological fertilizer and corresponding nutrient), remaining 1/2 nutrient is in just fruit
Phase topdresses administration.
Beneficial effect：
It is of the invention main to belong to bacterium using the Burkholder that Phos and rhizosphere effectively colonize that solve for separating
The administration of NJAU-B8, significantly improves the speed of growth of crops seedling stage, promotes crop to absorb nutrient in soil, so that
Promote plant growth.The composite microbiological fertilizer being made from it, using field after, significantly improve the product of field Different Crop
Amount.Promoting bacteria NJAU-B8 of the present invention 2 season pot experiment and 3 season field tests in show the growth-promoting and volume increase energy of stabilization
Power.Growth-promoting with effect of increasing production substantially, is adapted to field crop producing region large area and uses after microbiology class fertilizer product application of the present invention.
Brief description of the drawings
Fig. 1 is based on the phylogenetic tree that the 16S rDNA gene orders of bacterial strain NJAU-B8 are set up using adjacent method
Fig. 2 the 1st season corn pot test effect figures
Fig. 3 the 2nd season corn pot test effect figures
Fig. 4 first season field experiment corn yields
Note：Different letters represent different significant difference (Duncan-test, P in same row in figure<0.05), similarly hereinafter.
Fig. 5 second season field experiment Chinese cabbage yield
Fig. 6 third quarter field experiment potato yields
Biomaterial preservation information
Bacterial strain NJAU-B8, Classification And Nomenclature is Burkholderia bacterium (Bukhdderia sp.), is preserved in China micro-
Biological inoculum preservation administration committee common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
Institute of Microorganism, Academia Sinica, preservation date is on April 11st, 2016, and deposit number is CGMCC No.12339.
Specific embodiment
The separation and identification of embodiment 1, function stem
Corn root is gently rinsed into 2min under running water, removes rhizosphere surface mount soil.Then it is dry with aseptic water washing
Only, root is cut, adds with bead and be equipped with the 250mL conical flasks of 100mL sterilized waters, 25 DEG C, 170r/min vibrations
20min forms soil supension, and 10 are drawn respectively-2、10-3、10-4、10-5The soil supension 0.1mL of extension rate, coats inorganic
On phosphorus culture plate, 3 repetitions of each concentration, 28 DEG C culture 5 days after, selected on flat board around produce Soluble phosphorus circle bacterium colony it is pure
Change more than 5 times, the bacterium colony that selection still has Soluble phosphorus circle goes to inclined-plane culture, and 4 DEG C of preservations are standby.
Strain after purification is inoculated on the PVK culture mediums of improvement, after being placed in 30 DEG C of incubator cultures 7 days, measurement
Transparent circle and colony diameter, and calculate the ratio (D/d values) of transparent circle and colony diameter.
Selection transparent circle is relatively large in diameter or itself and the larger bacterial strain of colony diameter ratio, is inoculated into NBRIP culture mediums, 30
DEG C, 170r/min culture, 48h be measured by sampling nutrient solution in available phosphate concentration, after 7 days, determine nutrient solution in available phosphorus and full phosphorus
Content, if not connecing bacterium control, treatment is all provided with 3 repetitions with compareing.
Isolation medium (the PVK culture mediums after improvement) compound method wherein used is, as a example by preparing 1L culture mediums：Portugal
Grape sugar 10g, ammonium sulfate 0.5g, magnesium sulfate 0.3g, sodium chloride 0.3g, potassium chloride 0.2g, ferric sulfate 0.03g, manganese sulfate 0.03g,
Tricalcium phosphate 5g, distilled water 1000mL, pH 6.5~7.0.115 DEG C of sterilizing 30min.
Screening and culturing medium used use Plant Res Internat B. V.'s phosphate growth medium (NBRIP) compound method for, with
As a example by preparation 1L culture mediums：Glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, magnesium sulfate 0.25g, potassium chloride 0.2g, ammonium sulfate
0.1g, distilled water 1000mL, pH 7.0.115 DEG C of sterilizing 30min.
One plant of bacterium is finally obtained by qualitative and quantitative screening and is named as NJAU-B8, bacterial strain NJAU-B8 is on LB flat boards
Bacterium colony it is smaller, in faint yellow, and xanthein, edge-smoothing, tool can be produced to be a bit sticky, be difficult to provoke.Gram
It is negative staining, thalline uniform coloring.D-Fructose, maltose, arabinose, xylose, lactose utilization are positive, glucose fermentation
Produce acid not aerogenesis, 7%NaCl growths.With the constructed hair of the 16S rDNA sequences (accession number is KX421207) of NJAU-B8 bacterial strains
Educate tree table bright, it is same that bacterial strain NJAU-B8 belongs to bacterium Bukhdderia gladioli (EU024168) with Burkholderia gladioli
Source property reaches 99.1%, not same with Vietnam bulkholderia cepasea on taxology, morphological feature, physics and chemistry with reference to bacterial strain
Feature and 16S rDNA sequence analyses, Burkholderia bacterium is initially identified as by bacterial strain NJAU-B8.In being preserved in
State's Microbiological Culture Collection administration committee common micro-organisms center, deposit number is CGMCC No.12339.
Embodiment 2, function bacterium fills the generation of bacterium bacteria suspension
Liquid fermentation is produced during bacterial strain NJAU-B8 is seeded into LB nutrient solutions, and the condition of fermenting and producing is：PH 7.0, hair
30~35 DEG C of ferment temperature range, rotating speed is 170r/min, and fermentation time is 48h, make bacteria containing amount >=1.0 in zymotic fluid ×
1010CFU/mL；It is after zymotic fluid 6000r/min is centrifuged 8min, using the resuspended thalline of isometric 0.9% physiological saline to fill bacterium solution
Obtain bacteria suspension.
LB cultures liquid making method used is, as a example by preparing 1L culture mediums：Peptone 10g, dusty yeast 5g, NaCl
10g, agar 20g, are settled to 1000mL, pH natures, 121 DEG C of sterilizing 20min.
The development of embodiment 3, composite microbiological fertilizer
The composite microbiological fertilizer of described promotion crop yield application, 5 are pressed by common organic fertilizer and amino acid hydrolyticsolution:
1(w:V, kg：L) mix, by 20Kg fertilizer:The ratio addition NJAU-B8 zymotic fluids of 1L NJAU-B8 zymotic fluids, by secondary hair
Ferment, obtains biological organic fertilizer, addition nutrient chemical to total nitrogen (N), full phosphorus (P2O5) and full potassium (K2O) nutrient is respectively 80g
kg-1, 40gkg-1And 30gkg-1Composite microbiological fertilizer is developed into, its living bacteria count >=0.5 hundred million/g fertilizer.Chemical fertilizer
From urea (N, 46.6%), calcium superphosphate (P2O5, 12.0%), potassium chloride (K2O, 52.0%).Function bacterium be not inoculated with, is being adopted
Nutrient is added in the mixture being fermented into same process and forms organic-inorganic compound mixed fertilizer to consistent with composite microbiological fertilizer.
The application effect of embodiment 4, growth-promoting bio-fertilizer on corn potted plant (the 1st season, the 2nd season)
Two season pot experiment on June 2nd, 2014 to October 24 in Jiangsu Province's solid organic castoff recycling
Carried out in Hi-Tech Key Laboratory (Yixing) greenhouse, be Su Yu 29 for examination corn variety, purchased from academy of agricultural sciences of Jiangsu Province.Will
Corn seed is immersed in 4h in normal-temperature water, is placed on the gauze of moistening vernalization 2-3 days in 30 DEG C of incubators, treats more than 80%
After seed is sprouted, the seed that selection shows money or valuables one carries unintentionally moves to seedlings nursing plate nursery, and the consistent seedling replanting basin of growing way is selected after 2 true leaves are grown
In.Experiment sets two treatment：1) CK, only applies common organic fertilizer；2) NJAU-B8 is processed, using common organic fertilizer and NJAU-
The bacteria suspension of B8.Each 6 basin for the treatment of, the dress 2kg soil per basin, common organic fertilizer addition is 1.5% (mass percent, with 2kg soil
On the basis of), the filling bacterium amount of the bacteria suspension of bacterial strain NJAU-B8 preferably presses 2.5 × 107CFU/g soil (butt) is applied.Conventional pipe
Reason, determines that the plant height of plant, stem be thick, chlorophyll relative content measured value (Soil and plant respectively after 42d
Analyzerdevelopment, SPAD), overground part fresh weight, overground part dry weight.
1st season corn growth-promoting effect such as Fig. 2, biomass situation as shown in table 1, after potted plant culture 42d, compared to not connecing bacterium
Treatment, inoculating strain NJAU-B8 has growth-promoting effect substantially to maize seedling, and in addition to SPAD values, other growth indexes are significantly better than
CK(P<0.05), its plant height, stem be thick, chlorophyll, overground part fresh weight, overground part dry weight increase by 40.3% compared with CK respectively, 18.7%,
1.3%th, 76.4%, 76.7%.
2nd season corn growth-promoting effect such as Fig. 3, biomass situation are as shown in table 2, after potted plant culture 42d, using common organic
Caused after fertilizer and NJAU-B8 bacteria suspensions the overground part fresh weight and overground part dry weight of milpa be all remarkably higher than singly apply it is common organic
Fertile (CK) treatment (p<0.05).Compared with CK, the treatment using Chicken dung and NJAU-B8 bacteria suspensions is thick, on the ground in plant height, stem
Portion's fresh weight and overground part dry weight aspect, increased 15.3%, 1.9%, 6.2%, 9.9% respectively.
To sum up two seasons potted plant result shows, applies using common organic fertilizer and NJAU-B8 bacteria suspensions (NJAU-B8) treatment and singly
The corn plant height of common organic fertilizer (CK) treatment, difference substantially, illustrates bacterial strain NJAU- between overground part fresh weight, overground part dry weight
The growth-promoting effect of B8 is obvious.
Table 1, the 1st season corn growth-promoting pot experiment biomass situation
Note：SPAD：Chlorophyll relative content measured value；Data represent average value ± standard deviation in table.* represent different
Significant difference (Duncan-test,P<0.05).Similarly hereinafter.
Table 2, the 2nd season the potted plant growth-promoting test organism amount situation of corn
The application effect of embodiment 5, growth-promoting bio-fertilizer on crop field corn
Go up pot experiment effect according to this, bacterial strain NJAU-B8 is chosen to be developed into composite microbiological fertilizer and further carries out field
Between test.Field test is carried out in March, 2015 in April, 2016 in Haian County of Nantong City basic farmland, is for examination soil
Yellow soil, pH：6.83, the content of organic matter：9.63g·kg-1, total nitrogen content：2.29g·kg-1, content of tatal phosphorus：4.08g·kg-1, available phosphorus contents：21.1mg·kg-1, quick-acting potassium content：719mg·kg-1.Continuous 3 season of the plantation crop of field test：Corn,
Chinese cabbage and potato crop rotation.3 treatment are set altogether：1) chemical fertilizer control (CF)；2) organic-inorganic compound mixed fertilizer treatment (AOF):Do not connecing
Function bacterium is planted, is supported using addition in the mixture being fermented into the composite microbiological fertilizers of NJAU-B8 containing bacterial strain treatment same process
Divide to consistent with composite microbiological fertilizer and form organic-inorganic compound mixed fertilizer；3) composite microbiological fertilizers of NJAU-B8 containing bacterial strain treatment
(NJAU-B8).Each 3 repetition for the treatment of, each plot area is 30m2。
As shown in figure 4, the 1st season corn field test carried out in March, 2015 in August, 2015.Organic-inorganic compound mixed fertilizer
3000kghm is applied with biological organic fertilizer-2, remaining nutrient urea, calcium superphosphate and potassium sulfate are pressed total nutrient polishing, are supported
Distribution ratio is 8:4:3 (i.e. total nitrogen N, full phosphorus P2O5With full potassium K2O nutrients are respectively 80gkg-1, 40gkg-1And 30gkg-1)；Chemical fertilizer treatment directly applies urea, calcium superphosphate and the potassium sulfate of corresponding nutrient.2 administrations of fertilizer point, plant before seedling and apply always
The 1/2 of nutrient as base manure (including chemical fertilizer of all of organic-inorganic compound mixed fertilizer or composite microbiological fertilizer and corresponding nutrient),
Remaining 1/2 nutrient topdresses administration in first fruiting period.
1st season table of field test results it is bright：On the one hand, there was no significant difference for corn yield between respectively processing, it may be possible to soil
Nutrient is higher, masks the effect of microbial manure；On the other hand, NJAU-B8 treatment is increased production respectively than AOF and CF treatment
0.82% and 3.37%, the production-increasing function of this functions bacterium starts to manifest.
The application effect of embodiment 6, growth-promoting bio-fertilizer on the Chinese cabbage of crop crop field
As shown in figure 5, the 2nd season Chinese cabbage field test carried out in September, 2015 in December, 2015.Organic and inorganic compound
Fertilizer and biological organic fertilizer apply 3000kghm-2, remaining nutrient urea, calcium superphosphate and potassium sulfate press total nutrient polishing,
Nutrient formula is 8:4:3；Chemical fertilizer treatment directly applies urea, calcium superphosphate and the potassium sulfate of corresponding nutrient.Fertilizer point is applied for 2 times
With, plant before seedling apply the 1/2 of total nutrient as base manure (including all of organic-inorganic compound mixed fertilizer or composite microbiological fertilizer and
The chemical fertilizer of corresponding nutrient), remaining 1/2 nutrient topdresses administration in the knot luxuriant phase.
2nd season table of field test results it is bright：The Chinese cabbage yield of NJAU-B8 treatment is significantly higher than CF and AOF treatment (Fig. 5),
NJAU-B8 treatment increases production 3.92% and 5.74% respectively than AOF and CF treatment, and functions bacterium NJAU-B8 has significant volume increase
Effect.
The application effect of embodiment 7, growth-promoting bio-fertilizer on the potato of crop crop field
As shown in fig. 6, the 3rd season potato field test carried out in December, 2015 in May, 2016.Organic and inorganic compound
Fertilizer and biological organic fertilizer apply 3000kghm-2, remaining nutrient urea, calcium superphosphate and potassium sulfate press total nutrient polishing,
Nutrient formula is 8:4:8；Chemical fertilizer treatment directly applies urea, calcium superphosphate and the potassium sulfate of corresponding nutrient.Fertilizer point is applied for 2 times
With, plant before seedling apply the 1/2 of total nutrient as base manure (including all of organic-inorganic compound mixed fertilizer or composite microbiological fertilizer and
The chemical fertilizer of corresponding nutrient), remaining 1/2 nutrient topdresses administration in initial bloom stage.
3rd season table of field test results it is bright：The potato yield of NJAU-B8 treatment is significantly higher than CF and AOF treatment (Fig. 6),
NJAU-B8 treatment than AOF and CF treatment respectively increase production 12.73% and 35.89%, compared to the 2nd season rate of growth it is more significantly.
Claims (13)
1. one plant of burkholderia (Burkholderia) NJAU-B8, is preserved in Chinese microorganism strain preservation conservator
Meeting common micro-organisms center, preservation date is on April 11st, 2016, and deposit number is CGMCC No.12339.
2. the burkholderia NJAU-B8 described in claim 1 is in solution Phos and/or promotion plant growth and volume increase
Using；The preferred corn of plant, Chinese cabbage and potato.
3. the burkholderia NJAU-B8 described in claim 1 is preparing solution Phos, is promoting plant growth micro- with volume increase
Application in bacteria agent or composite microbiological fertilizer；The preferred corn of plant, Chinese cabbage and potato.
4. the microbial bacterial agent that prepared by the burkholderia NJAU-B8 described in claim 1.
5. microbial bacterial agent according to claim 4, it is characterised in that be mainly prepared by the following method：It is by preserving number
The burkholderia NJAU-B8 of CGMCC No.12339 is inoculated into LB fluid nutrient mediums, carries out liquid fermentation production, fermentation
The condition of production is：PH 6.0~7.0,30~35 DEG C of fermentation temperature scope, mixing speed is 170r/min, and fermentation time is
48h, makes bacteria containing amount >=1 × 10 in zymotic fluid10CFU/mL。
6. microbial bacterial agent according to claim 5, it is characterised in that conventionally make it after obtaining zymotic fluid
Standby is common formulations, is preferably prepared as pulvis, granule or suspending agent.
7. the composite microbiological fertilizer for being prepared as the burkholderia NJAU-B8 described in claim 1.
8. composite microbiological fertilizer according to claim 7, it is characterised in that be mainly prepared by the following method：By common
Organic fertilizer presses 5 with amino acid hydrolyticsolution:1(kg：L composite fertilizer) is mixed to obtain, by composite fertilizer:NJAU-B8 zymotic fluid mass volume ratios
It is 20:1(kg：L ratio), by secondary fermentation, obtains biological organic fertilizer to NJAU-B8 zymotic fluids are added in composite fertilizer, adds
Plus nutrient chemical is to total nitrogen N, full phosphorus P2O5With full potassium K2O nutrients are respectively 80gkg-1, 40gkg-1And 30gkg-1It is made
Composite microbiological fertilizer, wherein living bacteria count >=0.5 hundred million/g fertilizer.
9. application of the microbial bacterial agent described in claim 5 or 6 in solution Phos and/or promotion plant growth and volume increase；
The preferred corn of plant, Chinese cabbage and potato.
10. application according to claim 9, it is characterised in that the zymotic fluid described in claim 5 is fed directly to plant
Rhizosphere, or by described in claim 5 6000~8000r/min of zymotic fluid be centrifuged 8~10min after, using with fermentating liquid volume
The resuspended thalline of equal physiological saline is prepared into bacteria suspension, is then fed directly to the filling bacterium of plant rhizosphere, zymotic fluid or bacteria suspension
Amount is 2.0~3.0 × 10 preferably by bacterium solution volume and soil quality ratio7CFU/g soil (butt) is applied, and is further preferably pressed
According to 2.5 × 107CFU/g soil (butt) is applied.
Composite microbiological fertilizer any one of 11. claim 7-8 is in solution Phos and/or promotion plant growth and increasing
Application in product.
12. applications according to claim 11, its feature fertilization mode is composite microbiological fertilizer sowing amount 3000kg
hm-2, points of 2 times administrations of fertilizer plant before seedling and apply the 1/2 of total nutrient as base manure, and remaining 1/2 nutrient topdresses in first fruiting period and applies
With.
13. applications according to claim 11, it is characterised in that the plant is corn, Chinese cabbage and potato.
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