CN102816726A - Novel compound microbial agent and applications thereof - Google Patents
Novel compound microbial agent and applications thereof Download PDFInfo
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- CN102816726A CN102816726A CN2012103298992A CN201210329899A CN102816726A CN 102816726 A CN102816726 A CN 102816726A CN 2012103298992 A CN2012103298992 A CN 2012103298992A CN 201210329899 A CN201210329899 A CN 201210329899A CN 102816726 A CN102816726 A CN 102816726A
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Abstract
The invention discloses a novel compound microbial agent and relates to the technical field of agriculture microbes. 10 parts to 20 parts of bacillus subtilis, 20 parts to 30 parts of bacillus mucilaginosus and 50 parts to 70 pats of bacillus licheniformis serve as raw materials; and 5 parts to 15 parts of sodium humate, 30 parts to 50 parts of bran, 30 parts to 50 parts of rice bran and 5 parts to 15 parts of seaweed serve as raw materials for culture media. A preparation method of the novel compound microbial agent includes mixing and smashing the bacillus and maintaining the moisture to be in 30% to obtain the novel compound microbial agent. Bacterial manure prepared by mixing the novel compound microbial agent with nitrogen, phosphorus and potassium according to an appropriate ratio can effectively improve the granular structure of the soil and has a stimulation effect on the growth of crops, and the yield-increasing effect is apparent.
Description
Technical field
The present invention relates to agriculture microbial technology field, be specifically related to a kind of NEW TYPE OF COMPOSITE microbiobacterial agent.
Background technology
Complex microorganism is a quasi-microorganism formulation products that rises both at home and abroad in recent years, is characterized in through compound two or more microbial strains, can play multiple actions such as enhancing crop nutrition absorption, disease-resistant, impoverishment tolerant.Complex micro organism fungicide is by two or more and the mutual microbial preparation processed of the microbial strains of antagonism not, and this type of microbial inoculum has generally that kind is complete, compatibility is reasonable, functional good characteristics such as strong.But the stability of the complex microorganism preparations of using at present is not strong, be prone to affected by environment, group structure or make certain micro-organisms dead or get involved because of new mikrobe and to cause the community superiority of complex microorganism preparations to be changed because of environmental change.And complex micro organism fungicide is big owing to investment in research and development, generally is applied to aspects such as food, medicine, environmental treatment, and it is less to be applied to agricultural.
Subtilis (Bacillus subtilis (Ehrenberg) Cohn); Bacillus a kind of; The subtilyne that produces in its thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have the obvious suppression effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Can consume the free oxygen in the environment rapidly, cause low-oxygen environment, promote useful anerobes growth, and produce organic acid, suppress other pathogenic bacterium growth indirectly; Growing of immune stimulatory organ strengthens cellular immune function, improves herd immunity; Its thalline self synthesizes enzymes such as AMS, proteolytic enzyme, lypase, cellulase, together plays a role with digestive enzymes; Multiple vitamin B group such as ability synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising Interferon, rabbit and scavenger cell.
Gel-shaped bacillus (Bacillus mucilaginosus) is commonly called as the potassium bacterium, is a kind of soil bacteria common in the farmland.Early fifties, Soviet Union scholar Alexandria sieve husband at first reports and from soil, is separated to the special bacterium of this kind, and the plain ability of potassium in the activated silicates mineral is not only arranged, and can also utilize in the phosphatic rock the plain and fixing atmosphere nitrogen of phosphorus, thereby is called silicate bacteria.Colloid bacillus has the phosphorus potassium mineral and utilizes and resolving power, but the rapid available phosphorus potassium content that discharges at short notice is not high, and is particularly lower with the resolving power to potassium felspar sand, and special construction of rocks mineral is relevant therewith for this.Colloid bacillus also has nitrogen fixing capacity and PGPR effect (one type of bacterial flora that has the stimulating plant growth and suppress comprehensive actions such as phytopathogen in the plant rhizosphere life is called for short PGPR).
Bacillus licheniformis (Bacillus lincheniformis), bacillus a kind of, gram-positive microorganism; Cellular form and arrangement are shaft-like, Dan Sheng, can produce the various active enzyme, like proteolytic enzyme, glycase, lypase, polygalacturonase, LSD, cellulase etc.; Simultaneously can also produce the multiple enzymatic factor; Produce the activity resistent material, and have unique biological and take the oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacterium.Generally be applied to animal feedstuff additive, strengthen the activity of animal digestion enzyme, promote the growth of cultivated animals, can improve efficiency of feed utilization; Also can be applicable to medical science, reach therapeutic purpose, can impel body to produce antibacterial substance, kill pathogenic bacterium through the adjustment flora imbalance.
Summary of the invention
The object of the invention is to provide a kind of NEW TYPE OF COMPOSITE microbiobacterial agent, is mainly used in the production microbial-bacterial fertilizer.
The mikrobe that NEW TYPE OF COMPOSITE microbiobacterial agent of the present invention contains has:
(1) subtilis, Latin title are Bacillus subtilis (Ehrenberg) Cohn, Chinese bacterium name Chinese Name subtilis, Chinese agriculture microbial strains preservation administrative center, preserving number: ACCC.No.11060.
(2) bacillusmusilaginosiengineering, Latin title are Bacillus mucilaginosus, microbial strains preservation center, Gansu Province, preserving number: GSICC 30210.
(3) Bacillus licheniformis, Latin title are Bacillus lincheniformis, Chinese industrial microbial strains preservation administrative center, and preserving number is CICC.No.20166.
Technical scheme of the present invention is following:
Complex micro organism fungicide of the present invention, the material component weight part is following:
Subtilis 10-20 part,
Bacillusmusilaginosiengineering 20-30 part,
Bacillus licheniformis 50-70 part;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, the feed composition weight part is following:
Sodium salts humic acids 5-15 part,
Wheat bran 30-50 part,
Rice bran 30-50 part,
Marine alga 5-15 part.
Complex micro organism fungicide of the present invention, preferred feedstock component weight part is following:
Bacillus subtilis microbial agent 15-20 part,
Bacillusmusilaginosiengineering microbial inoculum 25-30 part,
Bacillus licheniformis agent 50-60 part;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, the preferred feedstock ingredients weight parts is following:
Sodium salts humic acids 8-13 part,
Wheat bran 35-45 part,
Rice bran 35-45 part,
Marine alga 8-13 part.
Complex micro organism fungicide of the present invention, most preferably the material component weight part is following:
20 parts of bacillus subtilis microbial agents,
30 parts of bacillusmusilaginosiengineering microbial inoculums,
50 parts of bacillus licheniformis agents;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, most preferably the feed composition weight part is following:
10 parts of Sodium salts humic acidss,
35 parts in wheat bran,
45 parts in rice bran,
10 parts of marine algas.
The preparation method of NEW TYPE OF COMPOSITE microbiobacterial agent of the present invention comprises following process step:
(1) preparation of bacillus subtilis microbial agent
1) Bacillus subtilis strain of test tube slant preserving process preservation is taken out, expands and be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivated maturation in 2-4 days after, subsequent use;
2) mature strains that step 1) is obtained is inoculated in nutrient solution, and this nutrient solution is with Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g, water 1000mL preparation, and pH is 7.2; Sterilize in the container containing; Shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter, is mature seed, gets into fermentation stage then; The amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) adopt starch 10kg, soybean cake powder 10kg, peptone 1kg, ammonium sulfate 1kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg to prepare fermented liquid; Regulating pH is 7.5; Water is settled to 1t, through high pressure steam sterilization, utilizes recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃; Remaining during this time in the jar is barotropic state, and gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank, control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; The same step 3) of culture condition is when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤30%, living bacteria count>=200,000,000/gram must the bacillus subtilis microbial agent product, and are subsequent use;
(2) preparation of bacillusmusilaginosiengineering microbial inoculum
1) the bacillusmusilaginosiengineering bacterial classification of test tube slant preserving process preservation is taken out, expands and be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivated maturation in 2-4 days after, subsequent use;
2) mature strains that step 1) is obtained is inoculated in nutrient solution; This nutrient solution uses that Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g, pH are 7.2, water 1000mL preparation, sterilizes in the container containing, and shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter; Be mature seed; Get into fermentation stage then, the amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) adopt starch 10kg, soybean cake powder 10kg, peptone 1kg, ammonium sulfate 1kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg to prepare fermented liquid, regulate pH 7.5, water is settled to 1t; Pass through high pressure steam sterilization; Utilize recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃, during be barotropic state in remaining jar, gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank; Control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; Culture condition is the same, when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram must bacillusmusilaginosiengineering microbial inoculum product, and are subsequent use;
(3) preparation of bacillus licheniformis agent
1) Bacillus licheniformis is transferred on fresh test tube beef extract-peptone slant medium from the test tube slant of preserving, cultivate 18h in 37 ℃;
2) mature strains that step 1) is obtained is inoculated in nutrient solution, and this nutrient solution is with Carnis Bovis seu Bubali cream 3g, peptone 5g, sodium-chlor 5g, agar 15g, zero(ppm) water 1000mL preparation, pH7.2; Sterilize in the container containing; Shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter, is mature seed, gets into fermentation stage then; The amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) prepare fermented liquid with steeping water 45kg, peptone 1.5kg, soybean cake powder 10kg, glucose 1.5kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg, regulate pH 7.5, water is settled to 1t; Pass through high pressure steam sterilization; Utilize recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃, during be barotropic state in remaining jar, gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank; Control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; Culture condition is the same, when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram must bacillusmusilaginosiengineering microbial inoculum product, and are subsequent use;
(4) complex micro organism fungicide preparation: the bacillus licheniformis agent that bacillus subtilis microbial agent that will (1) preparation, bacillusmusilaginosiengineering microbial inoculum and (3) of (2) preparation prepare is according to 10-20 part: 20-30 part: the mixed of 50-70 part, pulverizing; Moisture remains in 30%, promptly gets the NEW TYPE OF COMPOSITE microbiobacterial agent.
The present invention connects the complex micro organism fungicide that cultivation and fermentation absorption makes through expanding with subtilis (Bacillus subtilis (Ehrenberg) Cohn), bacillusmusilaginosiengineering (Bacillus mucilaginosus) and Bacillus licheniformis (Bacillus lincheniformis); Can effectively improve the crumb structure of soil with the microbial-bacterial fertilizer of processing after the suitable proportioning of nitrogen phosphorus potassium; And life, the activity of mikrobe in soil; Can produce a lot of meta-bolitess or secretory product; These secretory product have hormesis to the growth of crop, obvious effect of increasing production.
Specific embodiment
Below in conjunction with embodiment the present invention is elaborated, but protection scope of the present invention is not only for so.
Embodiment 1 preparation NEW TYPE OF COMPOSITE microbiobacterial agent
The feed composition weight part is following:
20 parts of bacillus subtilis microbial agents,
30 parts of bacillusmusilaginosiengineering microbial inoculums,
50 parts of bacillus licheniformis agents.
The substratum of NEW TYPE OF COMPOSITE microbiobacterial agent, ingredients weight parts is following:
10 parts of Sodium salts humic acidss,
35 parts in wheat bran,
45 parts in rice bran,
10 parts of marine algas;
The preparation method:
1) cultivation of single microbial inoculum
The bacterial classification that the test tube slant preserving process is preserved in 4 ℃ of refrigerators takes out, and expands to be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivates ripe in 2-4 days; Be inoculated in nutrient solution (Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g; PH7.2, water 1L) carrying out shaking table cultivates; Bacterium liquid bacterium number reaches the above entering one grade fermemtation stage of 2,000,000,000/milliliter, and the amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%.Adopt pressure differential method that seed liquor is pressed into fermentor tank, control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume; After treating that one grade fermemtation finishes, directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline, culture condition is the same; When fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches 2,000,000,000/milliliter when above, and fermented liquid is put into sterilized container for storing liquid; The aseptic peat composed of rotten mosses (water cut≤15%, fineness 80 mesh sieve percent of pass 80%) and fermented liquid are adsorbed with 3 to 5:1 ratios, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram get single microbial inoculum product.
2) complex micro organism fungicide preparation
The subtilis that will prepare respectively according to step 1), gel-shaped bacillus, Bacillus licheniformis are according to mixed, the pulverizing of 2:3:5; Moisture remains in 30%; Get complex micro organism fungicide, i.e. 20 parts of bacillus subtilis microbial agents, 30 parts of bacillusmusilaginosiengineering microbial inoculums, 50 parts of bacillus licheniformis agents.The substratum of this NEW TYPE OF COMPOSITE microbiobacterial agent is made up of 10 parts of Sodium salts humic acidss, 35 parts of wheat brans, 45 parts of rice brans, 10 portions of marine algas.
Embodiment 2 preparation NEW TYPE OF COMPOSITE microbiobacterial agents
The feed composition weight part is following:
15 parts of bacillus subtilis microbial agents,
30 parts of bacillusmusilaginosiengineering microbial inoculums,
55 parts of bacillus licheniformis agents.
The substratum of NEW TYPE OF COMPOSITE microbiobacterial agent, ingredients weight parts is following:
8 parts of Sodium salts humic acidss,
45 parts in wheat bran,
35 parts in rice bran,
12 parts of marine algas.
The preparation method is with embodiment 1.
embodiment 3: preparation peanut specific complex microbial-bacterial fertilizer, nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide content are respectively 19%, 6%, 13%, are mass percent.
The feed composition weight part is following:
8 parts of NEW TYPE OF COMPOSITE microbiobacterial agents,
8 parts of turfy soils,
40 parts in urea,
10 parts in fused(calcium magnesium)phosphate,
8 parts of monoammonium phosphates,
25 parts of vitriolate of tartar,
1 part of wilkinite;
The standard of peat composed of rotten mosses wood is water cut 2-5%, organic content >=30%;
Urea is small particle urea, and particle diameter is 0.85mm-2.80mm, and nitrogen content is 46.2%, is mass percent;
The content of Vanadium Pentoxide in FLAKES is 18% in the fused(calcium magnesium)phosphate, and the content of quicklime is 45%, and magnesian content is 12%, and the content of silicon oxide is 20%, is mass percent;
The total content of nitrogen is 10% in the monoammonium phosphate, and phosphorus pentoxide content is 50%, is mass percent;
Potassium oxide content is 51% in the vitriolate of tartar, is mass percent;
Wilkinite is to be main moisture clay pit with the smectite, owing to have special nature such as swelling property, cohesiveness, adsorptivity, catalytic, thixotropy, suspension and cationic exchange property, is widely used in each industrial circle, is referred to as in " omnipotent soil ";
The content that relates in the above raw material is mass percent.
The preparation method:
Above-mentioned raw materials NEW TYPE OF COMPOSITE microbiobacterial agent (taking from embodiment 1), turfy soil, urea, fused(calcium magnesium)phosphate, monoammonium phosphate, vitriolate of tartar, wilkinite weighed respectively place stirrer to mix 1.5 hours; Be under 8 the condition, to make the peanut microbial bacterial manure special at 30 ℃ of temperature, pH through granulation, oven dry, screening.
embodiment 4: preparation peanut specific complex microbial-bacterial fertilizer, nitrogen, Vanadium Pentoxide in FLAKES, potassium oxide content are respectively 18%, 9%, 11%, are mass percent.
The feed composition weight part is following:
10 parts of NEW TYPE OF COMPOSITE microbiobacterial agents,
8 parts of turfy soils,
35 parts in urea,
8 parts in fused(calcium magnesium)phosphate,
15 parts of monoammonium phosphates,
22 parts of vitriolate of tartar,
2 parts of wilkinites.
With embodiment 3, its preparation method is with embodiment 3 except that the NEW TYPE OF COMPOSITE microbiobacterial agent is taken from embodiment 2 for above material composition.
Comparative Examples: common 38% potassium sulfate compound fertilizer that Stanley Fertilizer Co.,Ltd produces.
The effect of above embodiment 3-4 and Comparative Examples is following:
Experimental cultivar: spend No. 8 in the Weihe River.
Supply examination soil: sandy loam, organic matter 0.88%, alkali-hydrolyzable nitrogen 68ppm, rapid available phosphorus 9.4ppm, available potassium 60ppm.Medium fertility.
Processing mode: each handles 10 square metres of sub-district areas, field random alignment, triplicate.
Sowing is handled: after stubble gathers in autumn before, and stubble-cleaning, the autumn is made new ridge after turning over, harrow, pressing.Bottom width 75-80cm, the high 5cm of furrow are made in the plot of film mulching and planting preparatively, the furrow of furrow face width 65-70cm, and it is wide to make 20-25cm in the middle of furrow and the furrow, the micro-ridge that 15cm is high, usefulness fetches earth during in order to sowing.Date of seeding is May 1, planting density 9000 pier/mus, and membrane covering, other field management is with general land for growing field crops.On September 9 harvesting time, after the results, the indoor peanut economic characters of carrying out are measured and the yield result analysis.
The effect of increasing production of peanut and quality improving effect are seen table 1.
Experimental result shows, compares with Comparative Examples, adopts the apply fertilizer peanut of proportioning of embodiment, and not only effect of increasing production is obvious, and fast, the seedling gesture of emerging strengthens.Wherein, the effect of increasing production of embodiment 3 is the most remarkable, is 23.9%; Embodiment 4 effect of increasing production are 19.8%.Therefore, the NEW TYPE OF COMPOSITE microbial bacteria agent prescription among the embodiment 1 is worthy to be popularized the most.
Claims (5)
1. complex micro organism fungicide is characterized in that the material component weight part is following:
Subtilis 10-20 part,
Bacillusmusilaginosiengineering 20-30 part,
Bacillus licheniformis 50-70 part;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, the feed composition weight part is following:
Sodium salts humic acids 5-15 part,
Wheat bran 30-50 part,
Rice bran 30-50 part,
Marine alga 5-15 part.
2. NEW TYPE OF COMPOSITE microbiobacterial agent according to claim 1 is characterized in that the material component weight part is following:
Bacillus subtilis microbial agent 15-20 part,
Bacillusmusilaginosiengineering microbial inoculum 25-30 part,
Bacillus licheniformis agent 50-60 part;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, the feed composition weight part is following:
Sodium salts humic acids 8-13 part,
Wheat bran 35-45 part,
Rice bran 35-45 part,
Marine alga 8-13 part.
3. NEW TYPE OF COMPOSITE microbiobacterial agent according to claim 1 or claim 2 is characterized in that the material component weight part is following:
20 parts of bacillus subtilis microbial agents,
30 parts of bacillusmusilaginosiengineering microbial inoculums,
50 parts of bacillus licheniformis agents;
The substratum of said NEW TYPE OF COMPOSITE microbiobacterial agent, the feed composition weight part is following:
10 parts of Sodium salts humic acidss,
35 parts in wheat bran,
45 parts in rice bran,
10 parts of marine algas.
4. the preparation method of NEW TYPE OF COMPOSITE microbiobacterial agent according to claim 1 is characterized in that comprising following process step:
(1) preparation of bacillus subtilis microbial agent
1) Bacillus subtilis strain of test tube slant preserving process preservation is taken out, expands and be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivated maturation in 2-4 days after, subsequent use;
2) mature strains that step 1) is obtained is inoculated in nutrient solution, and this nutrient solution is with Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g, water 1000mL preparation, and pH is 7.2; Sterilize in the container containing; Shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter, is mature seed, gets into fermentation stage then; The amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) adopt starch 10kg, soybean cake powder 10kg, peptone 1kg, ammonium sulfate 1kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg to prepare fermented liquid; Regulate pH 7.5; Water is settled to 1t, through high pressure steam sterilization, utilizes recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃; Remaining during this time in the jar is barotropic state, and gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank, control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; The same step 3) of culture condition is when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤30%, living bacteria count>=200,000,000/gram must the bacillus subtilis microbial agent product, and are subsequent use;
(2) preparation of bacillusmusilaginosiengineering microbial inoculum
1) the bacillusmusilaginosiengineering bacterial classification of test tube slant preserving process preservation is taken out, expands and be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivated maturation in 2-4 days after, subsequent use;
2) mature strains that step 1) is obtained is inoculated in nutrient solution; This nutrient solution uses that Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g, pH are 7.2, water 1000mL preparation, sterilizes in the container containing, and shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter; Be mature seed; Get into fermentation stage then, the amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) adopt starch 10kg, soybean cake powder 10kg, peptone 1kg, ammonium sulfate 1kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg to prepare fermented liquid, regulate pH 7.5, water is settled to 1t; Pass through high pressure steam sterilization; Utilize recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃, during be barotropic state in remaining jar, gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank; Control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; Culture condition is the same, when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram must bacillusmusilaginosiengineering microbial inoculum product, and are subsequent use;
(3) preparation of bacillus licheniformis agent
1) Bacillus licheniformis is transferred on fresh test tube beef extract-peptone slant medium from the test tube slant of preserving, cultivate 18h in 37 ℃;
2) mature strains that step 1) is obtained is inoculated in nutrient solution, and this nutrient solution is with Carnis Bovis seu Bubali cream 3g, peptone 5g, sodium-chlor 5g, agar 15g, zero(ppm) water 1000mL preparation, and pH is 7.2; Sterilize in the container containing; Shaking table is cultured to bacterium liquid bacterium number and reaches more than 2,000,000,000/milliliter, is mature seed, gets into fermentation stage then; The amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%;
3) prepare fermented liquid with steeping water 45kg, peptone 1.5kg, soybean cake powder 10kg, glucose 1.5kg, potassium primary phosphate 0.5kg, yeast powder 0.1kg, lime carbonate 5.0kg, regulate pH 7.5, water is settled to 1t; Pass through high pressure steam sterilization; Utilize recirculated cooling water that fermented liquid is cooled to 28 ℃-30 ℃, during be barotropic state in remaining jar, gauge pressure is controlled at 0.05Mpa; Adopt pressure differential method that seed liquor is pressed into fermentor tank; Control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume;
4) treat that one grade fermemtation finishes after; Directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline; Culture condition is the same, when fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches more than 2,000,000,000/milliliter, when the gemma rate is 80%, is fermentation ends; Fermented liquid is put into sterilized container for storing liquid, and is subsequent use;
5) with the aseptic peat composed of rotten mosses of water cut≤15%, fineness 80 mesh sieve percent of pass 80% and fermented liquid with 3-5: the absorption of 1 ratio, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram must bacillusmusilaginosiengineering microbial inoculum product, and are subsequent use;
(4) complex micro organism fungicide preparation: the bacillus licheniformis agent that bacillus subtilis microbial agent that will (1) preparation, bacillusmusilaginosiengineering microbial inoculum and (3) of (2) preparation prepare is according to 10-20 part: 20-30 part: the mixed of 50-70 part, pulverizing; Moisture remains in 30%, promptly gets the NEW TYPE OF COMPOSITE microbiobacterial agent.
5. NEW TYPE OF COMPOSITE microbiobacterial agent according to claim 1, the application in preparation peanut specific complex microbial-bacterial fertilizer.
Priority Applications (1)
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| CN103497047A (en) * | 2013-09-18 | 2014-01-08 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Aquiculture fertilizer with antimicrobial and disinfecting functions and preparation method thereof |
| CN103772047A (en) * | 2014-01-26 | 2014-05-07 | 贵州昌源蓖麻发展有限公司 | Organic compound fertilizer employing castor bean meal and microbial bacteria and preparation method of organic compound fertilizer |
| CN104402650A (en) * | 2014-10-29 | 2015-03-11 | 黑龙江盛瑞康生物科技开发有限公司 | Microbial inoculant powder for rice and corn plantation |
| CN104557247A (en) * | 2013-10-28 | 2015-04-29 | 北京中农赛世农业科技有限公司 | Vegetable seedling-raising culture composition and application |
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| CN108203330A (en) * | 2018-02-24 | 2018-06-26 | 广东嘉美好生态科技有限公司 | Alga oligosaccharides microbial bacterial agent |
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| CN108513988A (en) * | 2018-03-27 | 2018-09-11 | 湖南省蓝正微生物科技开发有限公司 | Microorganism composite pesticide |
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| CN103497047A (en) * | 2013-09-18 | 2014-01-08 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Aquiculture fertilizer with antimicrobial and disinfecting functions and preparation method thereof |
| CN104557247A (en) * | 2013-10-28 | 2015-04-29 | 北京中农赛世农业科技有限公司 | Vegetable seedling-raising culture composition and application |
| CN103772047A (en) * | 2014-01-26 | 2014-05-07 | 贵州昌源蓖麻发展有限公司 | Organic compound fertilizer employing castor bean meal and microbial bacteria and preparation method of organic compound fertilizer |
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| CN104402650A (en) * | 2014-10-29 | 2015-03-11 | 黑龙江盛瑞康生物科技开发有限公司 | Microbial inoculant powder for rice and corn plantation |
| CN105001021A (en) * | 2015-08-04 | 2015-10-28 | 沈阳鑫嘉捷生物制剂有限公司 | Total-element compound microorganism organic fertilizer and preparing technology thereof |
| CN105441370A (en) * | 2016-01-29 | 2016-03-30 | 山东和田旺生物科技有限公司 | Microbial agent and preparing method thereof |
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| CN106396862A (en) * | 2016-08-29 | 2017-02-15 | 佛山市艳晖生物科技有限公司 | Disease controlling microbial fertilizer special for tuberous crops and preparation method thereof |
| CN107056513A (en) * | 2017-06-27 | 2017-08-18 | 山东昆仲知识产权代理有限公司 | Microbial bacteria agent and process for producing same |
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| CN108203330A (en) * | 2018-02-24 | 2018-06-26 | 广东嘉美好生态科技有限公司 | Alga oligosaccharides microbial bacterial agent |
| CN108513988A (en) * | 2018-03-27 | 2018-09-11 | 湖南省蓝正微生物科技开发有限公司 | Microorganism composite pesticide |
| CN108913617A (en) * | 2018-06-29 | 2018-11-30 | 上海创博生态工程有限公司 | A kind of biological agent and preparation method thereof of efficient-decomposition organic waste |
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| CN109593679A (en) * | 2018-12-26 | 2019-04-09 | 大连雪龙都市农业有限公司 | A kind of nanometer selenium microbial bacterial agent and the preparation method and application thereof |
| CN109734484A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of microorganism formulation and preparation method thereof conducive to salt-soda soil plant growth |
| CN110498719A (en) * | 2019-07-29 | 2019-11-26 | 胡玉红 | For improveing microbial manure and its application of soil |
| CN110498719B (en) * | 2019-07-29 | 2023-04-11 | 胡玉红 | Microbial fertilizer for improving soil and application thereof |
| CN112794774A (en) * | 2021-01-13 | 2021-05-14 | 南宁市拜欧生物工程有限责任公司 | Composite organic liquid biological bacterial fertilizer applied to grape planting and preparation method thereof |
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