CN107556093A - A kind of special hybrid bacterial strain microbial manure of solanaceous vegetables and preparation method thereof - Google Patents
A kind of special hybrid bacterial strain microbial manure of solanaceous vegetables and preparation method thereof Download PDFInfo
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- CN107556093A CN107556093A CN201710656825.2A CN201710656825A CN107556093A CN 107556093 A CN107556093 A CN 107556093A CN 201710656825 A CN201710656825 A CN 201710656825A CN 107556093 A CN107556093 A CN 107556093A
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Abstract
The present invention relates to special hybrid bacterial strain microbial manure of a kind of solanaceous vegetables and preparation method thereof.Belong to field of agricultural microbial technology.It is characterized in that：In parts by weight, raw material components include charcoal powder 30~35%, turf 25~30%, edible fungi residues：6~10%, humic acid 6~10%, potassium fulvate 1~3%, rare earth 1~3%, bacillus mycoides Bacillus mycoides Gnyt1 bacterial strain fermentation liquors 10%, bacillus subtilis Bacillus subtilis FX2 bacterial strain fermentation liquors 10%.The hybrid bacterial strain microbial manure, with nitrogen in fixed air, dissolve the effect of insoluble phosphorus in soil, comprehensive solanaceous vegetables, equilibrium, the nutrient easily absorbed can be supplied to, on the other hand, bacterial strain in microbial manure has the ability of secretion plant hormone ability and antagonism pathogen, can adjust the growth of solanaceous vegetables, and pre- disease prevention occurs.
Description
Technical field
The present invention relates to field of agricultural microbial technology, and in particular to a kind of micro- life of the special hybrid bacterial strain of solanaceous vegetables
Thing fertilizer and preparation method thereof.
Background technology
Solanaceous vegetables refers generally to the vegetables using berry as Leaf-feeding insects in plant of Solanaceae, common are eggplant, tomato,
Capsicum etc..High, nutritious, the resistance to resistance to fortune of storage of solanaceous vegetables fruiting period length, strong adaptability, yield is the people in summer and autumn
The staple vegetable in season.Solanaceous vegetables is one of maximum vegetables of China's cultivated area, accounts for more than the 20% of the vegetables gross area, entirely
There is cultivation state various regions.
China is the big country of world's solanaceous vegetables plantation and consumption.However, research finds that solanaceous vegetables is to nitrogen, phosphorus
The dependence of fertilizer is higher, and by taking tomato as an example, 100 kilograms of tomatoes of production need 0.4 kilogram of nitrogen, 0.45 kilogram of phosphorus, in actual production,
Peasant is to pursue high yield, the usage amount severe overweight of chemical fertilizer.Investigation display, solanaceous vegetables plant every batch of fertilizer application amount
More than 100 kgs/acre, so substantial amounts of chemical fertilizer not only increases the input of peasant's purchase property, also creates the rings such as soil degradation
Border problem, meanwhile, also bring hidden danger to food security.Therefore, how to reduce fertilizer amount is in current solanaceous vegetables production
Important topic.
In recent years, research showed that plant rhizosphere, which has a large amount of Promoting bacterias, (can promote a major class of plant growth both at home and abroad
Bacterium), as the phosphorus element for being difficult to directly be absorbed by plant in soil can be converted into plant available state by phosphorus-solubilizing bacteria；Nitrogen-fixing bacteria
Nitrogen in air can be fixed as plant available state by (being divided into symbiosis, joint and growing nitrogen-fixing)；Secreting plant hormone bacterium can
Secrete plant hormone (such as auxin, gibberellin, the basic element of cell division)；Cause of disease Antagonistic Fungi (also known as biocontrol microorganisms) can suppress or kill
Dead phytopathogen etc..Meanwhile some Promoting bacterias have the characteristics such as Soluble phosphorus, fixed nitrogen and secretion plant hormone concurrently.With from plant rhizosphere
The complex inoculant (or bacterial manure) that is developed into of efficient Promoting bacteria that separation obtains, not only can with activating soil Insoluble phosphates, Gu
Determine the nitrogen in air, there is provided nitrogen, phosphorus nourishing, while absorption of the crops to other nutrients can be promoted, improve and plant
Thing trophic structure, regulation and promotion plant growth, strengthen plant disease-resistant ability.In addition, Promoting bacteria can also improve soil texture, carry
High soil organic matter content, improvement salt-soda soil, prevent soil degradation etc..Therefore, carry out the research of plant growth-promoting bacteria resource and answer
Closing Inoculant (bacterial manure) research and development has extremely important realistic meaning.
Advocate production safety, non-harmful pollution-free food all in actively development green agriculture both at home and abroad now.It is green producing
Then require not having in color food products or as far as possible less with (or limitation uses) chemical fertilizer, chemical pesticide and other chemical substances.
It requires that fertilizer is protected and promotes subject to grow and improve quality first；Next to that subject is not caused to produce and accumulate
Harmful substance；Third, ecological environment is had no adverse effects.
It is most micro- although there is more microbial manure to have been applied in agricultural production practice in the prior art
Bio-feritlizer is unreasonable because strain forms, or preparation manipulation is improper, its effect is nothing like chemical fertilizer, therefore, market is recognized
It can spend very low.
The content of the invention
The main object of the present invention is to be directed to both at home and abroad in the prior art, depending on unduly in relevant solanaceous vegetables plantation
Fertilizer, cause fertilizer application efficiency to reduce and cause the present situation of ecological environmental pollution problem, explore, excavate and utilize newly micro-
Living resources, save the energy and environmental protection.The technology of the present invention screens excellent growth-promoting bacterial strain, with certain formula test solanaceous vegetables
Special compound microbial fertilizer for vegetables, the hybrid bacterial strain microorganism fertilizer of new " environmentally friendly " is provided for solanaceous vegetables production
Material, partly to substitute industrial chemical fertilizer, while prevent solanaceous vegetables soil-borne disease from invading, reduce the use and agriculture of chemical fertilizer, agricultural chemicals
The purchase input of the people.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
A kind of solanaceous vegetables Special compound microbial fertilizer, in parts by weight, its raw material components include charcoal powder
30~35%, turf 25~30%, edible fungi residues：6~10%, humic acid 6~10%, potassium fulvate 1~3%, rare earth 1~
3%, bacillus mycoides Bacillus mycoides Gnyt1 bacterial strain fermentation liquor 10%, bacillus subtilis Bacillus
Subtilis FX2 bacterial strain fermentation liquors 10%.
Wherein, 2 plants of bacterium are described as follows：
Bacillus mycoides Bacillus mycoides Gnyt1 strain isolations are from Tianzhu Area, Gansu county Alpine Grasslands serpentgrass
(Polygonum viviparum L.) rhizosphere, there is Soluble phosphorus, fixed nitrogen, secretion plant hormone, the ability of antagonistic phytopathogen；
China typical culture collection center is preserved on April 10th, 2017, in the preservation of Wuhan University of address wuchang, wuhan area
The heart, numbering are CCTCC M 2017177；
Bacillus subtilis Bacillus subtilis FX2 strain isolations from clover (Medicago sativa) rhizosphere,
With Soluble phosphorus, fixed nitrogen, secretion plant hormone, antagonistic phytopathogen ability.Chinese allusion quotation is preserved on April 10th, 2017
Type culture collection, Wuhan University of address wuchang, wuhan area collection, numbering is CCTCC M 2017178.
This hybrid bacterial strain can grow on containing less than 5% NaCl culture medium；The hybrid bacterial strain system of determination is shown, four plants
Without Antagonistic reaction between bacterium, in every measure such as Soluble phosphorus, fixed nitrogen, secretion plant hormone (IAA, GA)：After compound strain,
Organic phosphorous content increases by 75.24 μ gL-1, 596.32 μ gL of content of inorganic phosphorus increase-1, nitrogenase activity 3358.71nmol
C2H4h-1mL-1, IAA contents are 79.84 μ gmL-1, GA3 contents are 67.63 μ gmL-1, to pathogen (Fusarium
Oxysporum inhibiting rate) is 93.75%, using compared with each bacterial strain individually culture, each index has substantially after compound strain
Increase.
Pass through experiment, it was demonstrated that bacillus mycoides does not have antagonism not only between each other when being mixed with bacillus subtilis
Effect, and also have the effect mutually promoted.
Table 1：Composite bacteria agent contrasts with single strain microbial inoculum using effect
Carrier compound is：The raw materials such as charcoal powder, turf, edible fungi residues, humic acid, potassium fulvate, rare earth are according to 35：25：
8：8：2：2 ratio fully mixes.
Further, the bacterial strain fermentation liquor viable bacteria concentration is more than 1.0 × 109cfu/mL。
Further, the composite microbiological fertilizer preparation method is：
(1) add water-water reactor to macerate edible fungi residues, heat-preservation fermentation, make to dry after its is decomposed standby；
(2) raw materials such as charcoal powder, turf, edible fungi residues, humic acid, potassium fulvate, rare earth are cleaned after crushing respectively, mistake
2mm is sieved；
(3) each raw material is fully mixed；
(4) by the carrier of above-mentioned mixing at 121 DEG C, 0.1MPa autoclave sterilizations 1h is standby after cooling down；
(5) NA fluid nutrient mediums are prepared, are inoculated with bacillus mycoides and bacillus subtilis, 28~30 DEG C of cultures 24~48
Hour, thalline quantity in nutrient solution is calculated under the microscope with blood counting chamber, when quantity is more than 1.0 × 109It is whole during cfu/mL
Only cultivate, 2 kinds of bacterium solutions are fully mixed；
(6) by cultured bacterium solution according to carrier:Bacterium solution is 8:2 ratio is inoculated in sterilized carrier, and sterile
Under the conditions of be placed in 28~30 DEG C culture 5 days i.e. can be used, or in sterile sealed containers normal temperature preservation it is stand-by.
Benefit of the invention is that：
(1) the hybrid bacterial strain microbial manure, there is nitrogen in fixed air, dissolve the effect of insoluble phosphorus in soil,
Comprehensive solanaceous vegetables, equilibrium, the nutrient easily absorbed can be supplied to, on the other hand, the bacterial strain in microbial manure, which has, to be divided
The ability of plant hormone ability and antagonism pathogen is secreted, the growth of solanaceous vegetables can be adjusted, pre- disease prevention occurs.Not only such as
This, the carrier nutrition used in the hybrid bacterial strain microbial manure is balanced, has regulation soil pH, increases the effect of soil fertility.
(2) the carrier physicochemical property used in the hybrid bacterial strain microbial manure is excellent, is advantageous to colonizing and depositing for microorganism
It is living；And the carrier nutrition is balanced, inherently play the role of to increase soil fertility；With the exception of this, carrier also has regulation soil pH
Effect, the improvement for salt-soda soil has good effect.
(3) the special hybrid bacterial strain microbial manure organic manure application rate of overcoming over of the solanaceous vegetables is big, quality not
The shortcomings that stable, administration is very convenient.Soil physico-chemical property caused by also overcoming pure chemistry fertilizer simultaneously is degenerated and agricultural production
The shortcomings that quality declines.
(4) the special hybrid bacterial strain microbial manure of the solanaceous vegetables playing antagonism and suppress its life to pathogenic microorganism
The effect of long breeding, reaches the effect of mitigating crop disease, improves the quality and yield of crops and the ability of anti-continuous cropping.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition suggested according to normal condition or manufacturer are carried out.Agents useful for same or the unreceipted production firm person of instrument, it is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
The special hybrid bacterial strain microbial manure of solanaceous vegetables described in the present embodiment, in parts by weight, it is former
Material component includes charcoal powder 35%, turf 25%, edible fungi residues 8%, humic acid 8%, potassium fulvate 2%, and rare earth 2% is excellent
Bacterial strain fermentation liquor 20%.The liquid bacterial agent strain excellent zymotic fluid is by bacillus mycoides Bacillus mycoides
Gnyt1 bacterial strain fermentation liquors, bacillus subtilis Bacillus subtilis FX2 bacterial strain fermentation liquors press 1:1 ratio composition.
The preparation method of above-mentioned hybrid bacterial strain microbial manure is as follows：
(1) add water-water reactor to macerate edible fungi residues, heat-preservation fermentation, make to dry after its is decomposed standby；
(2) raw materials such as charcoal powder, turf, edible fungi residues, humic acid, potassium fulvate, rare earth are cleaned after crushing respectively, mistake
2mm is sieved；
(3) each raw material is fully mixed；
(4) by the carrier of above-mentioned mixing at 121 DEG C, 0.1MPa autoclave sterilizations are standby after cooling down within 1 hour；
(5) NA fluid nutrient mediums, inoculation bacillus mycoides and 2 kinds of bacterial strains of bacillus subtilis, 28~30 DEG C of trainings are prepared
Support 24~48 hours, thalline quantity in nutrient solution is calculated under the microscope with blood counting chamber, when quantity is more than 109During cfu/mL
Culture is terminated, 2 kinds of bacterium solutions are fully mixed；
(6) by cultured bacterium solution according to carrier:Bacterium solution is 8:2 ratio is inoculated in sterilized carrier, and sterile
Under the conditions of be placed in 28~30 DEG C culture 5 days i.e. can be used, or in sterile sealed containers normal temperature preservation it is stand-by.
The special hybrid bacterial strain microbial manure of the solanaceous vegetables of embodiment 1 is subjected to field test, from tomato, eggplant
Three kinds of son, capsicum solanaceous vegetableses, its yield and the content of vitamin C (Vc) are determined respectively, verify the eggplant of the embodiment of the present invention 1
The field efficacy of the special hybrid bacterial strain microbial manure of fruit type vegetable, as a result such as following table：
As seen from the above table, using the special hybrid bacterial strain microbial manure of solanaceous vegetables of the embodiment of the present invention 1, Ke Yiyou
Effect improves the yield of tomato, eggplant, capsicum etc. and the content of vitamin C (Vc), illustrates that it can improve the yield of solanaceous vegetables
With the content of nutritional ingredient, in addition, practice process is additionally observed that, the special mixing of the solanaceous vegetables of the inventive embodiments 1 is used
Strain microorganism fertilizer, can shorten the maturity period of vegetables, and resist infecting for disease fungus in soil, prevent soil-borne disease
Occur.
The special hybrid bacterial strain microbial manure of solanaceous vegetables being prepared through this method stores at room temperature, 6 months
After detect its viable count, up to 5.15 × 108cfu/g.Meet《People's Republic of China's agricultural industry criteria (NY/T 1113-
2006)》Regulation.
Embodiment 2：
The invention provides a kind of tomato Special compound microbial fertilizer, and in parts by weight, its raw material components includes
Charcoal powder 30%, turf 30%, edible fungi residues：10%, humic acid 6%, potassium fulvate 3%, rare earth 1s%, bacillus mycoides
Bacillus mycoides Gnyt1 bacterial strain fermentation liquors 10%, bacillus subtilis Bacillus subtilis FX2 bacterial strains hair
Zymotic fluid 10%.
The preparation method of above-mentioned hybrid bacterial strain microbial manure is as follows：
(1) add water-water reactor to macerate edible fungi residues, heat-preservation fermentation, make to dry after its is decomposed standby；
(2) raw materials such as charcoal powder, turf, edible fungi residues, humic acid, potassium fulvate, rare earth are cleaned after crushing respectively, mistake
2mm is sieved；
(3) each raw material is fully mixed；
(4) by the carrier of above-mentioned mixing at 121 DEG C, 0.1MPa autoclave sterilizations are standby after cooling down within 1 hour；
(5) NA fluid nutrient mediums, inoculation bacillus mycoides and 2 kinds of bacterial strains of bacillus subtilis, 28~30 DEG C of trainings are prepared
Support 24~48 hours, thalline quantity in nutrient solution is calculated under the microscope with blood counting chamber, when quantity is more than 109During cfu/mL
Culture is terminated, 2 kinds of bacterium solutions are fully mixed；
(6) by cultured bacterium solution according to carrier:Bacterium solution is 8:2 ratio is inoculated in sterilized carrier, and sterile
Under the conditions of be placed in 28~30 DEG C culture 5 days i.e. can be used, or in sterile sealed containers normal temperature preservation it is stand-by.
The special hybrid bacterial strain microbial manure of the solanaceous vegetables of embodiment 2 is subjected to field test, from tomato, eggplant
Three kinds of son, capsicum solanaceous vegetableses, its yield and the content of vitamin C (Vc) are determined respectively, verify the eggplant of the embodiment of the present invention 2
The field efficacy of the special hybrid bacterial strain microbial manure of fruit type vegetable, as a result such as following table：
As seen from the above table, using the special hybrid bacterial strain microbial manure of solanaceous vegetables of the embodiment of the present invention 2, Ke Yiyou
Effect improves the yield of tomato, eggplant, capsicum etc. and the content of vitamin C (Vc), illustrates that it can improve the yield of solanaceous vegetables
With the content of nutritional ingredient, in addition, practice process is additionally observed that, the special mixing of the solanaceous vegetables of the inventive embodiments 2 is used
Strain microorganism fertilizer, can shorten the maturity period of vegetables, and resist infecting for disease fungus in soil, prevent soil-borne disease
Occur.
The special hybrid bacterial strain microbial manure of solanaceous vegetables being prepared through this method stores at room temperature, 6 months
After detect its viable count, up to 5.17 × 108cfu/g.Meet《People's Republic of China's agricultural industry criteria (NY/T 1113-
2006)》Regulation.
Claims (3)
- A kind of 1. special hybrid bacterial strain microbial manure of solanaceous vegetables, it is characterised in that in parts by weight, raw material group Dividing includes charcoal powder 30~35%, turf 25~30%, edible fungi residues：6~10%, humic acid 6~10%, potassium fulvate 1~ 3%, rare earth 1~3%, bacillus mycoides Bacillus mycoides Gnyt1 bacterial strain fermentation liquors 10%, bacillus subtilis Bacillus subtilis FX2 bacterial strain fermentation liquors 10%.
- A kind of 2. special hybrid bacterial strain microbial manure of solanaceous vegetables according to claim 1, it is characterised in that institute Liquid bacterial agent viable bacteria concentration is stated more than 109cfu/mL。
- A kind of 3. special hybrid bacterial strain microbial manure of solanaceous vegetables according to claim 1, it is characterised in that system Preparation Method is：(1) add water-water reactor to macerate edible fungi residues, heat-preservation fermentation, make to dry after its is decomposed standby；(2) raw materials such as charcoal powder, turf, edible fungi residues, humic acid, potassium fulvate, rare earth are cleaned after crushing respectively, crosses 2mm Sieve；(3) each raw material is fully mixed；(4) by the carrier of above-mentioned mixing at 121 DEG C, 0.1MPa autoclave sterilizations 1h is standby after cooling down；(5) NA fluid nutrient mediums, inoculation bacillus mycoides and 2 kinds of bacterial strains of bacillus subtilis, 28~30 DEG C of cultures 24 are prepared ~48 hours, thalline quantity in nutrient solution is calculated under the microscope with blood counting chamber, when quantity is more than 109Terminated during cfu/mL Culture, 2 kinds of bacterium solutions are fully mixed；(6) by cultured bacterium solution according to carrier:Bacterium solution is 8:2 ratio is inoculated in sterilized carrier, and in aseptic condition Under be placed in 28~30 DEG C culture 5 days i.e. can be used, or in sterile sealed containers normal temperature preservation it is stand-by.
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| CN108300668A (en) * | 2018-01-26 | 2018-07-20 | 重庆鹓雏农业科技有限公司 | Organic matter speciality fermenting agent |
| CN108409507A (en) * | 2018-06-13 | 2018-08-17 | 东营市昶盛农业科技有限公司 | A kind of preparation method of the applicable Lenlinus edodes residue organic fertilizer of round eggplant |
| CN108715821A (en) * | 2018-05-25 | 2018-10-30 | 商丘职业技术学院 | A kind of complex microbial inoculum and its preparation method and application |
| CN110447660A (en) * | 2019-08-08 | 2019-11-15 | 甘肃农业大学 | A kind of microbial bacteria inhibitor and preparation method thereof |
| CN110818477A (en) * | 2019-09-16 | 2020-02-21 | 甘肃农业大学 | Special bacterial fertilizer for allium mongolicum regel and preparation method thereof |
| CN111418611A (en) * | 2020-04-01 | 2020-07-17 | 甘肃省农业科学院植物保护研究所 | Growth-promoting and disease-preventing microbial agent special for barley crops and preparation method and application thereof |
| CN113667617A (en) * | 2021-08-06 | 2021-11-19 | 甘肃农业大学 | Special microbial agent for natural high-cold grassland and preparation method and application thereof |
| CN116120127A (en) * | 2022-11-30 | 2023-05-16 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
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| CN108300668A (en) * | 2018-01-26 | 2018-07-20 | 重庆鹓雏农业科技有限公司 | Organic matter speciality fermenting agent |
| CN108300668B (en) * | 2018-01-26 | 2021-08-17 | 重庆鹓雏农业科技有限公司 | Organic matter special fermentation bacterial agent |
| CN108715821A (en) * | 2018-05-25 | 2018-10-30 | 商丘职业技术学院 | A kind of complex microbial inoculum and its preparation method and application |
| CN108715821B (en) * | 2018-05-25 | 2021-09-10 | 商丘职业技术学院 | Microbial compound inoculant and preparation method and application thereof |
| CN108409507A (en) * | 2018-06-13 | 2018-08-17 | 东营市昶盛农业科技有限公司 | A kind of preparation method of the applicable Lenlinus edodes residue organic fertilizer of round eggplant |
| CN110447660A (en) * | 2019-08-08 | 2019-11-15 | 甘肃农业大学 | A kind of microbial bacteria inhibitor and preparation method thereof |
| CN110818477A (en) * | 2019-09-16 | 2020-02-21 | 甘肃农业大学 | Special bacterial fertilizer for allium mongolicum regel and preparation method thereof |
| CN111418611A (en) * | 2020-04-01 | 2020-07-17 | 甘肃省农业科学院植物保护研究所 | Growth-promoting and disease-preventing microbial agent special for barley crops and preparation method and application thereof |
| CN113667617A (en) * | 2021-08-06 | 2021-11-19 | 甘肃农业大学 | Special microbial agent for natural high-cold grassland and preparation method and application thereof |
| CN116120127A (en) * | 2022-11-30 | 2023-05-16 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
| CN116120127B (en) * | 2022-11-30 | 2024-02-02 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
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Application publication date: 20180109 |