CN108513988A - Microorganism composite pesticide - Google Patents
Microorganism composite pesticide Download PDFInfo
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- CN108513988A CN108513988A CN201810256407.9A CN201810256407A CN108513988A CN 108513988 A CN108513988 A CN 108513988A CN 201810256407 A CN201810256407 A CN 201810256407A CN 108513988 A CN108513988 A CN 108513988A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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Abstract
The invention discloses a kind of microorganism composite pesticides, include microbial fermentation mixed liquor 90 94 by mass parts；Synergist 6 10；Microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax printing fabric fermentation, bacillus megaterium zymotic fluid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid；Synergist includes fresh amine ester, calcium acetate, zinc sulfate, ammonium nitrate, chitosan, Tea Saponin and urea.The present invention is mixed using the strain of different Pseudomonas, and different Pseudomonas have selectivity to different pest and disease damages, can prevent a variety of pest and disease damages；Especially there is preferable control effect to the various insects of Lepidoptera, Orthoptera, Diptera, coleoptera, Semiptera, Hymenoptera and Ephemerida；And pest is not likely to produce drug resistance, protects pest natural enemy, active balance ecological environment.
Description
Technical field
The present invention relates to pesticide fields, and in particular to a kind of microorganism composite pesticide.
Background technology
Currently, environmental pollution is largely caused in countries in the world using chemical pesticide, many chemical pesticides do not have choosing because of it
Selecting property and threat also resulted in beneficial biology, while many chemical pesticides are non-degradables or are difficult degradation, are easy dynamic
Plant interior accumulation and the enrichment for polluting object.
Microbial pesticide is the NPD projects of 21 century pesticide industry, chemical pesticide can be overcome to the pollution of ecological environment and
Reduce in agricultural and sideline product persticide residue, while using microbial pesticide disease-prevention and insecticide production agricultural and sideline product quality and
Price will significantly rise, and advantageously facilitate rural economy growth and increasing peasant income, social benefit are inestimable.It is existing to occur
A large amount of microbial insecticide, the microbial insecticide as disclosed in application number CN201410442008.3, microorganism therein are adopted
Thuringiensis bacillus liquid, muscardine bacterium solution, photosynthetic bacteria liquid, saccharomycete bacterium solution, tobacco leaf chelating liquid, potassium dihydrogen phosphate,
Diethyl aminoethyl hexanoate, compound sodium nitrophenolate and organosilicon, the microorganism used for the microorganism of different Pseudomonas, under same environment its effect have
Institute is different, therefore the pest kind of its effect also has certain specific aim.Disclosed in CN201510103305.X
The microbial insecticide composition of red fire ant, the muscardine used and thiacloprid mixing are prevented, but thiacloprid is chemical pesticide,
Also it be easy to cause residual.Microbial insecticide mainly uses single culture or the mixing of the strain of different Pseudomonas at present, or with chemistry
Pestsides synthesis, bactericidal effect reduces, and chemical residual is higher.
Therefore, the development, industrialization and popularization and application process for further speeding up microbial pesticide, reduce pesticide in agricultural and sideline production
The great sustainable Control of Diseases and Insect Pests of crops is realized in residual in product and the pollution to farmland ecological environment, meets China without public affairs
Evil agricultural product industrialization production will generate huge society, economy and ecological benefits to the great demand of agricultural science and technology.
Invention content
The present invention proposes a kind of microorganism composite pesticide, the microorganism of bacillus is mainly used, with synergist
It is used cooperatively, good disinsection effect, noresidue is environmentally protective.
To achieve the goals above, the present invention is achieved through the following technical solutions：
A kind of microorganism composite pesticide includes microbial fermentation mixed liquor 90-94 by mass parts；Synergist 6-10；
The microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 30-36, withered grass gemma according to mass parts
Bacillus fermentation liquid 11-15, bacillus amyloliquefaciens zymotic fluid 12-18, wax printing fabric zymotic fluid 7-11, bacillus megaterium
Zymotic fluid 8-12, the lichen bacillus ferments liquid 6-10, bacillus polymyxa zymotic fluid 10-14；
The synergist according to mass parts include the fresh 32-38 of amine ester, calcium acetate 3-7, zinc sulfate 6-10, ammonium nitrate 10-15,
Chitosan 12-18, Tea Saponin 5-9, urea 15-19.
Preferably, the microbial fermentation mixed liquor according to mass parts include bacillus thuringiensis zymotic fluid 30-35, it is withered
Careless fermentation of bacillus liquid 12-14, bacillus amyloliquefaciens zymotic fluid 13-16, wax printing fabric zymotic fluid 8-10, huge bud
Spore bacillus fermentation liquid 8-10, the lichen bacillus ferments liquid 6-8, bacillus polymyxa zymotic fluid 10-12.
Preferably, the microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 33, withered grass according to mass parts
Fermentation of bacillus liquid 13, bacillus amyloliquefaciens zymotic fluid 15, wax printing fabric zymotic fluid 9, bacillus megaterium fermentation
Liquid 10, the lichen bacillus ferments liquid 8；Bacillus polymyxa zymotic fluid 12.
Preferably, the synergist includes that amine ester fresh 35, calcium acetate 5, zinc sulfate 8, ammonium nitrate 13, shell are poly- according to mass parts
Sugar 15, Tea Saponin 7, urea 17.
Preferably, in the bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens hair
Strain content is in zymotic fluid, wax printing fabric zymotic fluid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid
Hundred million cfu/mL of 50-80.
Preferably, strain content is hundred million/g of 80-100 in the bacillus megaterium zymotic fluid.
The preparation method of microorganism composite pesticide includes the following steps：The microbial fermentation mixed liquor of mass parts is passed through into pressure
Force difference sucks in finished pot, and under conditions of rotating speed is 120r/min after rotation 20-30min, the synergist of mass parts is added
Continue to be stirred until homogeneous suspension.
The preparation method of bacillus thuringiensis zymotic fluid is：
(1) bacillus thuringiensis strain is seeded in inoculation medium and is cultivated, inoculation medium is at 121 DEG C
Sterilizing 30 minutes, bacillus thuringiensis strain, inoculated and cultured can be inoculated with by waiting in tank that inoculation medium is cooled to 34 DEG C or so
The formula of base is：Soya-bean cake slurry 1.0%, yeast extract 0.6%, peptone 0.2%, dipotassium hydrogen phosphate 0.1%, calcium carbonate 0.4%, nothing
Water magnesium sulfate 0.03%, ferrous sulfate heptahydrate 0.03%, soya-bean oil 0.2%, wherein pH7.0~7.2 of inoculation medium；
(2) under aseptic technique, 30ml sterile waters are added in the inoculation medium to inoculation, it is outstanding that bacterium is made
Liquid, then bacteria suspension is transferred to the serum bottle of sterilizing, under alcolhol burner flame, the bacteria suspension in serum bottle is inhaled with depressurizing inocalation method
Enter in seeding tank and cultivated, at 30 ± 1 DEG C, tank presses 0.5Mpa, ventilatory capacity 1 for cultivation temperature control in seeding tank：1 (volume/body
Product/point), gas must be totally without miscellaneous bacteria, mixing speed 240r/min.Ventilatory capacity is gradually increased after culture 2 hours, through 6~8
Hour, Vegetative growth to logarithmic phase checks that sterile is certified seed liquid.
(3) cultured seed liquor is accessed in fermentation tank again and is fermented, fermentation tank culture medium is equipped in fermentation tank,
Fermentation tank culture medium sterilizes 30 minutes at 121 DEG C and is cooled to 30 DEG C, then seed liquor is accessed in fermentation tank culture medium, seed liquor
Inoculum concentration be fermentation medium quality 9%.The formula of the fermentation tank culture medium is：Soyabean cake pulp-water 2.0%, glucose
1%, yeast extract O.6%, peptone 0.8%, calcium carbonate 0.2%, epsom salt 0.005%, potassium dihydrogen phosphate 0.1%, seven
Aqueous ferrous sulfate 0.005%, white vitriol 0.005%, maltose 0.5%, soya-bean oil 0.2%, surplus is distilled water.Fermentation tank is trained
The pH for supporting base is 7.2~7.5.
Fermentation condition and management：Cultivation temperature control presses 0.3~0.5Mpa in 1 DEG C of 30 scholar, tank, and gas must be totally without miscellaneous
Bacterium, mixing speed are 240r/min ventilatory capacities 1:1 (volume/volume/point).Entire fermentation process answers timing sampling inspection, measures
The content and pH value of sugar, nitrogen, phosphorus must take the measures such as feed supplement to be adjusted if there is excessively high too low phenomenon.Culture 20~22 is small
When, it is 50~8,000,000,000 cfu/mL, as bacillus thuringiensis zymotic fluid containing bacterium number on inspection without miscellaneous bacteria.
The preparation method of bacillus subtilis fermentation liquor is：
(1) strain of refrigeration is inoculated on slant medium, for 24 hours, wherein inclined-plane culture based formulas is for 30 DEG C of cultures：It is small
Ox medicinal extract 3g, peptone 5g, NaCl5g, 15~20g of agar, water 1L, pH7.0.
(2) seed is scraped from slant medium with sterile water, and appropriate access is taken to fill 100ml aseptic seeds
In the 300ml conical flasks of culture medium, shaken cultivation is for 24 hours in 30 DEG C, the shaking table of 150r/min.The wherein formula of seed culture medium
For：Beef extract 3g, peptone 10g, glucose 8g, NaCl5g, starch 30g, magnesium sulfate 0.031g, calcium carbonate 0.2, distilled water
1L；Seed culture medium sterilizes 30 minutes at 121 DEG C, waits for that seed culture medium is cooled to 33 DEG C or so and can be inoculated with, and in seed
It is cultivated in culture medium.Cultivation temperature control is at 34 ± 1 DEG C, ventilatory capacity 1：1 (volume/volume/point), gas must clean nothing
Miscellaneous bacteria, mixing speed 140r/min.Ventilatory capacity is gradually increased after culture 2 hours, through 6~8 hours, Vegetative growth to logarithm
Phase checks that sterile is certified seed liquid.
(3) it will ferment in qualified seed liquor access fermentation tank, there is fermentation medium, fermentation medium in fermentation tank
It sterilizes 30 minutes at 121 DEG C and is seeded to seed liquor wherein when being cooled to 30 DEG C；The inoculum concentration of the seed liquor is fermented and cultured
The 9% of matrix amount.The formula of fermentation medium is：It is glucose 2%, peptone 1.5%, sodium chloride 0.5%, molasses 5%, solvable
Property starch 3%, remainder be distilled water；PH value 7.2；
Fermentation condition and management：Cultivation temperature is controlled at 32 ± 1 DEG C, and mixing speed 140r/min, gas must be clean
Without miscellaneous bacteria, ventilatory capacity 1:1~1.5 (volume/volume/point).Entire fermentation process answers timing sampling inspection, measures sugar, nitrogen, phosphorus
Content and pH value must take the measures such as feed supplement to be adjusted if there is excessively high too low phenomenon.Culture 20-22 hours, on inspection without miscellaneous
Bacterium is 50~8,000,000,000 cfu/mL, as bacillus subtilis fermentation liquor containing bacterium number.
The preparation method of wax printing fabric zymotic fluid：
(1) wax printing fabric is cultivated to a period of time on slant medium, the formula of wherein slant medium is：Egg
White peptone 1%, yeast extract 0.5%, glucose 0.1%, magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.02%, remaining is distilled water；
PH7.0；
(2) seed is scraped from slant medium with sterile water, and appropriate access is taken to fill 100ml aseptic seeds
In the 500ml conical flasks of culture medium, the formula of seed culture medium is：Glucose 2%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate
0.3%, magnesium sulfate 0.02%, calcium chloride 0.01%, remaining distilled water.PH value 7~7；It shakes in 32 DEG C, the shaking table of 200r/min
Culture 20h, 8000r/min centrifugation 20min is swung, takes supernatant liquor spare；Under aseptic technique, above-mentioned to depressurize inocalation method
It is cultivated in centrifugal clear liquid sucking seeding tank, cultivation temperature control is at 34 ± 1 DEG C, ventilatory capacity 1：1 (volume/volume/point), gas must
It must be totally without miscellaneous bacteria, mixing speed 140r/min.Ventilatory capacity is gradually increased after culture 2 hours, and through 6~8 hours, trophosome was given birth to
Length checks that sterile is certified seed liquid to logarithmic phase；
(3) seed liquor is accessed in fermentation tank and is fermented, there is fermentation medium, fermentation medium to exist in fermentation tank
121 DEG C sterilize 30 minutes and are seeded to seed liquor wherein when being cooled to 30 DEG C；The inoculum concentration of the seed liquor is fermentation medium
The 9% of quality.The formula of fermentation medium is：Glucose 2%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate
0.02%, calcium chloride 0.01%, remaining distilled water；PH value 7.2；
Fermentation condition and management：Cultivation temperature is controlled at 32 ± 1 DEG C, and mixing speed 140r/min, gas must be clean
Without miscellaneous bacteria, ventilatory capacity 1:1 (volume/volume/point).Entire fermentation process answers timing sampling inspection, measures the content of sugar, nitrogen, phosphorus
And pH value must take the measures such as feed supplement to be adjusted if there is excessively high too low phenomenon.Culture 20~22 hours, on inspection without miscellaneous bacteria,
It is 50~8,000,000,000 cfu/mL, as wax printing fabric zymotic fluid containing bacterium number.
The preparation method of bacillus megaterium zymotic fluid：
Directly add water constant volume in fermentation tank, is passed through air in advance, culture medium is direct plungeed into tank and is stirred, using direct
Air inlet method sterilizes, and material temperature is directly raised to 121 DEG C in disinfection, and tank pressure maintains 0.2MPa, is kept for 30 minutes；Disinfection terminates direct
By interlayer into water cooling；It will ferment in 1% part of bacillus megaterium seed liquor access seeding tank, the seed culture time is 5 small
When；The inoculum concentration of seed liquor is 9% access ferment tank of fermentation medium quality；Seeding tank and fermentation tank culture condition：
Wind ratio is 1: 1, and tank pressure is 0.5MPa, and temperature is 37 ± 2 DEG C, and the fermentation tank culture time is 10-18 hours, on inspection without miscellaneous bacteria,
It is 80~10,000,000,000/g containing bacterium number；As bacillus megaterium zymotic fluid.
The culture medium prescription is：Wheat wheat-middlings 0.8%, fish meal 0.8%, calcium carbonate 0.12%, ammonium sulfate 0.05%, phosphorus
Sour hydrogen dipotassium 0.04%, water 92.1%.
The preparation method of the lichen bacillus ferments liquid is：
Directly add water constant volume in fermentation tank, is passed through air in advance, culture medium is direct plungeed into tank and is stirred；Using direct
Air inlet method sterilizes, and material temperature is directly raised to 121 DEG C in disinfection, and tank pressure maintains 0.2MPa, is kept for 30 minutes；Disinfection terminates direct
Then it ventilates pressurize, is carried out at the same time cooling, ventilation ratio is adjusted to 1 when temperature reaches most suitable cultivation temperature (37 DEG C):1, it connects
Enter seed, it is 200rpm to start stirring, obtains seed liquor；It will be sent out in 1% part of bacillus licheniformis seed liquor access seeding tank
Ferment, seed culture time are 5 hours；By the 8% access ferment tank that the inoculum concentration of seed liquor is fermentation medium quality；Kind
Sub- tank and fermentation tank culture condition：Wind ratio is 1:1, tank pressure is 0.5MPa, and temperature is 37 ± 2 DEG C, and pH value cannot be below 6.5, hair
Fermentation tank incubation time is 10-18 hours, is 50~8,000,000,000 cfu/mL, as bacillus licheniformis containing bacterium number on inspection without miscellaneous bacteria
Zymotic fluid.
The preparation method of bacillus amyloliquefaciens zymotic fluid：
(1) culture medium：Yeast extract 5.0g, tryptone 6.0g, glucose 7.0g, agar 15.0g, sterile distilled water
1000ml；PH is adjusted to 7.0, suitably in cooling post package to test tube, in test tube after culture medium heating is dissolved, mixed well
Loading amount be the 1/5~1/6 of test tube, be stoppered test tube mouth with plug, 121 DEG C of moist heat sterilizations 30 minutes take out test tube and are placed on while hot
Aseptic operating platform sets inclined-plane, is cooled to 35 DEG C and bacillus amyloliquefaciens parent species is taken to be inoculated with, the test tube being inoculated with is put into insulating box
Activation culture is for 24 hours in 32~34 DEG C；
(2) in the 300mlYPG culture solutions for taking the bacterium immigration 1000ml triangles after being activated in one of test tube bottled,
It is cultivated 48 hours at 120r/min, 28 DEG C, obtains bacterium solution；The formula of YPG culture solutions is：Yeast extract 5.0g, tryptone
6.0g, glucose 7.0g, sterile distilled water 1000ml；PH7.0, loading amount is 1/3~1/4 in triangular flask, and triangle is stoppered with plug
Bottleneck, 121 DEG C of moist heat sterilizations inoculate for 30 minutes；
(3) fermentation medium is adjusted into the pH to 7.0 in fermentation medium as in fermentation tank, charging aperture is sealed, with height
Warm steam sterilizing 2 hours, it is cooling；The inoculum concentration of seed liquor is seeded in fermentation tank for the 9% of fermentation medium quality and cultivates 24
~36 hours；When occurring more foam during the fermentation, it should antifoaming agent be added, the antifoaming agent is organosilicon, is added
The foam being subject in seeding tank or fermentation tank is measured not spill over；On inspection without miscellaneous bacteria, it is 50~8,000,000,000 cfu/mL containing bacterium number, that is, solves
Bacillus amyloliquefaciens zymotic fluid；
Fermentative medium formula described above is：Glucose 0.75%, molasses 2%, dried silkworm chrysalis meal 1%, corn steep liquor 2%, chlorine
Change sodium 0.2%, calcium carbonate 0.8%, magnesium sulfate 0.1%, manganese sulfate 0.01%, ferrous sulfate 0.003g/L, remainder is distilled water；
pH7.0。
The preparation method of bacillus polymyxa zymotic fluid is：
(1) culture medium：Beef extract 4.0g, it tryptone 9g, glucose 2.0g, agar 15.0g, eats salt 5g, is sterile
Distilled water 1000ml；PH is adjusted to 7.2 after culture medium heating is dissolved, mixed well, suitably in cooling post package to test tube,
Loading amount in test tube is the 1/5-1/6 of test tube, and test tube mouth is stoppered with plug, and 121 DEG C of moist heat sterilizations 30 minutes take out test tube while hot
It is placed on aseptic operating platform and sets inclined-plane, be cooled to 35 DEG C and bacillus amyloliquefaciens parent species is taken to be inoculated with, the test tube being inoculated with is put into perseverance
Activation culture is for 24 hours in 32~34 DEG C for incubator；
(2) in the 300mlYPG culture solutions for taking the bacterium immigration 1000ml triangles after being activated in one of test tube bottled,
It is cultivated 24 hours at 120r/min, 28 DEG C, obtains bacterium solution；The formula of YPG culture solutions is：Beef extract 5.0g, tryptone 9g,
Glucose 2.0g, edible salt 5g, sterile distilled water 1000ml；PH7.2 is adjusted, loading amount is 1/3~1/4 in triangular flask, uses plug
It is stoppered triangle bottleneck, 121 DEG C of moist heat sterilizations inoculate for 30 minutes；
(3) fermentation medium is put into fermentation tank, is sufficiently stirred, fermentation medium pH is adjusted to 7.2, sealing charging
Hole is inoculated with bacterium solution, inoculum concentration 15% immediately with high-temp steam sterilizing 2 hours after being cooled to 35 DEG C of coolings；It is cultivated in fermentation tank
Directly add water constant volume after 20h in fermentation tank, be passed through air in advance, various raw materials are weighed up, direct plunges into stirring in tank；Using
Material temperature, is directly raised to 121 DEG C by direct air inlet method disinfection in disinfection, tank pressure maintains 0.2MPa, is kept for 30 minutes；Disinfection terminates
Direct pressurize of then ventilating, is carried out at the same time cooling, ventilation ratio is adjusted to 1 when temperature reaches most suitable cultivation temperature (37 DEG C):
1, it is 170rpm to start stirring,；By 15% that the inoculum concentration of seed liquor is fermentation medium quality by the seed liquor in seeding tank
Access ferment tank；Seeding tank and fermentation tank culture condition：Tank pressure is 0.5MPa, and temperature is 37 ± 2 DEG C, and pH value cannot be below
6.5, the fermentation tank culture time is 24-36 hours, is 50~8,000,000,000 cfu/ml containing bacterium number on inspection without miscellaneous bacteria；As more viscous buds
Spore bacillus fermentation liquid.
Microorganism composite pesticide of the present invention mainly inhibits pathogen energy production, interference biosynthesis and destroys cell knot
Structure.Absorbability is strong, toxicity is low, and what is played the role of has stimulation plant growth concurrently.Bacterium is low to nutritional requirement, and has easily in plant table
The characteristics of face colonizes, and its is large number of, reproduction speed is fast, therefore plays an important roll in terms of plant protection.It can prevent
The sprout term disease of vegetable at protected field and muskmelon prevents cucumber and Colletotrichumtabacum, prevents cabbage black rot, prevents rice banded sclerotial blight
Disease etc..
Microorganism composite pesticide of the present invention, advantage are：
(1) present invention is mixed using the strain of different Pseudomonas, and different Pseudomonas have selectivity to different pest and disease damages,
It can prevent a variety of pest and disease damages；Especially to the more of Lepidoptera, Orthoptera, Diptera, coleoptera, Semiptera, Hymenoptera and Ephemerida
Kind insect has preferable control effect；And pest is not likely to produce drug resistance, protects pest natural enemy, active balance ecological environment.
(2) present invention is living microorganism bacterium colony and the compound pesticide of plant emulsified refined oil, and specificity is strong, microorganism or generation
It thanks to product and is all directed to certain special cause of diseases as controlling object, this so that non-target organism is comparatively safe, and side effect is reduced.
(3) Environmental security, live body or microorganism itself in microbial pesticide are present in nature, it passes through metabolism, ginseng
It is recycled with energy and substance, biological concentration phenomenon will not be caused, environment and food security influenced small；
(4) synergist is compound, the introducing of the synergist can not only increase microbial pesticide cooperate with, be complementary, promoting and
Synergy, synergistic effect is notable, can greatly increase insecticidal effect, and agriculture can also be increased to reduce cost by reducing pesticide dosage
Crop fertilizer efficiency, makes increasing crop yield increase income；The benefit of desinsection is greatly reinforced, and compared to other microbial pesticides, the present invention
Pesticide is quick, quick disease-prevention and insecticide.
Specific implementation mode
With reference to specific embodiment, present invention be described in more detail.
Embodiment 1
A kind of microorganism composite pesticide includes microbial fermentation mixed liquor 92, synergist 8 by mass parts；
Microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 36, fermentation of bacillus subtilis according to mass parts
Liquid 11, bacillus amyloliquefaciens zymotic fluid 12, wax printing fabric zymotic fluid 11, bacillus megaterium zymotic fluid 8, lichens gemma
Bacillus fermentation liquid 10, bacillus polymyxa zymotic fluid 10；
Synergist includes amine ester fresh 38, calcium acetate 7, zinc sulfate 6, ammonium nitrate 10, chitosan 12, Tea Saponin according to mass parts
5, urea 15；
In bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax-like bud
Strain content is hundred million cfu/ of 50-80 in born of the same parents' bacillus fermentation liquid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid
mL；Strain content is hundred million/g of 80-100 in bacillus megaterium zymotic fluid.
The preparation method of microorganism composite pesticide includes the following steps：The microbial fermentation mixed liquor of mass parts is passed through into pressure
Force difference sucks in finished pot, and under conditions of rotating speed is 120r/min after rotation 20-30min, the synergist of mass parts is added
Continue to be stirred until homogeneous suspension.
Embodiment 2
A kind of microorganism composite pesticide includes microbial fermentation mixed liquor 90, synergist 10 by mass parts；
Microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 30, fermentation of bacillus subtilis according to mass parts
Liquid 15, bacillus amyloliquefaciens zymotic fluid 18, wax printing fabric zymotic fluid 10, bacillus megaterium zymotic fluid 10, lichens bud
Spore bacillus fermentation liquid 10, bacillus polymyxa zymotic fluid 14；
Synergist includes amine ester fresh 35, calcium acetate 5, zinc sulfate 10, ammonium nitrate 15, chitosan 15, tea soap according to mass parts
Element 9, urea 18；
In bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax-like bud
Strain content is hundred million cfu/ of 50-80 in born of the same parents' bacillus fermentation liquid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid
mL；Strain content is hundred million/g of 80-100 in bacillus megaterium zymotic fluid.
The preparation method of microorganism composite pesticide includes the following steps：The microbial fermentation mixed liquor of mass parts is passed through into pressure
Force difference sucks in finished pot, and under conditions of rotating speed is 120r/min after rotation 20-30min, the synergist of mass parts is added
Continue to be stirred until homogeneous suspension.
Embodiment 3
A kind of microorganism composite pesticide includes microbial fermentation mixed liquor 92, synergist 8 by mass parts；
Microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 33, fermentation of bacillus subtilis according to mass parts
Liquid 13, bacillus amyloliquefaciens zymotic fluid 15, wax printing fabric zymotic fluid 9, bacillus megaterium zymotic fluid 10, lichens gemma
Bacillus fermentation liquid 8；Bacillus polymyxa zymotic fluid 12；
Synergist includes amine ester fresh 35, calcium acetate 5, zinc sulfate 8, ammonium nitrate 13, chitosan 15, Tea Saponin according to mass parts
7, urea 17；
In bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax-like bud
Strain content is hundred million cfu/ of 50-80 in born of the same parents' bacillus fermentation liquid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid
mL；Strain content is hundred million/g of 80-100 in bacillus megaterium zymotic fluid.
The preparation method of microorganism composite pesticide includes the following steps：The microbial fermentation mixed liquor of mass parts is passed through into pressure
Force difference sucks in finished pot, and under conditions of rotating speed is 120r/min after rotation 20-30min, the synergist of mass parts is added
Continue to be stirred until homogeneous suspension.
Embodiment 4
A kind of microorganism composite pesticide includes microbial fermentation mixed liquor 94, synergist 6 by mass parts；
Microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 33, fermentation of bacillus subtilis according to mass parts
Liquid 13, bacillus amyloliquefaciens zymotic fluid 15, wax printing fabric zymotic fluid 9, bacillus megaterium zymotic fluid 10, lichens gemma
Bacillus fermentation liquid 8；Bacillus polymyxa zymotic fluid 12；
Synergist includes amine ester fresh 35, calcium acetate 5, zinc sulfate 8, ammonium nitrate 13, chitosan 15, Tea Saponin according to mass parts
7, urea 17；
In bacillus thuringiensis zymotic fluid, bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax-like bud
Strain content is hundred million cfu/ of 50-80 in born of the same parents' bacillus fermentation liquid, the lichen bacillus ferments liquid, bacillus polymyxa zymotic fluid
mL；Strain content is hundred million/g of 80-100 in bacillus megaterium zymotic fluid.
The preparation method of microorganism composite pesticide includes the following steps：The microbial fermentation mixed liquor of mass parts is passed through into pressure
Force difference sucks in finished pot, and under conditions of rotating speed is 120r/min after rotation 20-30min, the synergist of mass parts is added
Continue to be stirred until homogeneous suspension.
Embodiment 5
The application of microorganism composite pesticide of the present invention：
(1) vegetables
Main prevention：Cabbage caterpillar, diamondback moth, beans Jia snout moth's larvas, leaf miner and nocturnal moth class.20-25 DEG C of dispenser optimum temperature, spray
Best Times：Early morning and dusk, 150-250ml/ mus of dosage.Control effect is up to 80-90%.In addition, 5 hours after spray meet
The characteristics of rain can promote drug effect to play, can using big yield spraying instead at high temperature season (30 DEG C or so temperature), (field spraying per acre is used
150 pounds/acre of water), it advocates and is sprayed using small machine, control effect is made to significantly improve.
(2) cotton
Main prevention：A generation, two generations, three generations's bollworm dosage：300-450ml/ mus, in ovum peak period 2-3 (worm of spraying
The hair rate high age).Control effect protects flower bud effect 74% up to 75-90%.
(3) corn
Main prevention：Corn borer dosage：It 150ml/ mus, is applied in lobus cardiacus in corn lobus cardiacus end, in egg stage of Ostrinia furnacalis height
Peak early period, i.e. beginning peak period start to spray.Control effect is up to 77.3-91.6%.
(4) rice
Main prevention：Generation striped rice borer, three generations yellow rice borer, rice plant skipper, rice leaf roller.Dosage：300ml/ mus, effect
For 89-91%.And after use, rice leaf turns dark green by pale green.
(5) gardens, fruit tree
Main prevention：Slug moth, decline greatly moth, and citrus leaf-miner slug moth and the big moth that declines are Leaf-feeding Insects, dilute 200-300 times
Bt emulsions, spray after larvae hatch in a couple of days, and control effect is up to 90%.Citrus leaf-miner is with 300-600 times of Bt breast
Agent, control effect is up to 85%.
It is provided for the embodiments of the invention technical solution above to be described in detail, specific case used herein
The principle and embodiment of the embodiment of the present invention are expounded, the explanation of above example is only applicable to help to understand this
The principle of inventive embodiments；Meanwhile for those of ordinary skill in the art, embodiment according to the present invention, in specific embodiment party
There will be changes in formula and application range, in conclusion the content of the present specification should not be construed as limiting the invention.
Claims (7)
1. a kind of microorganism composite pesticide, it is characterised in that：Include microbial fermentation mixed liquor 90-94 by mass parts；Synergist
6-10；
The microbial fermentation mixed liquor includes bacillus thuringiensis zymotic fluid 30-36, bacillus subtilis according to mass parts
Zymotic fluid 11-15, bacillus amyloliquefaciens zymotic fluid 12-18, wax printing fabric zymotic fluid 7-11, bacillus megaterium fermentation
Liquid 8-12, the lichen bacillus ferments liquid 6-10, bacillus polymyxa zymotic fluid 10-14；
The synergist includes that the fresh 32-38 of amine ester, calcium acetate 3-7, zinc sulfate 6-10, ammonium nitrate 10-15, shell are poly- according to mass parts
Sugared 12-18, Tea Saponin 5-9, urea 15-19.
2. microorganism composite pesticide according to claim 1, it is characterised in that：The microbial fermentation mixed liquor is according to quality
Part includes bacillus thuringiensis zymotic fluid 30-35, bacillus subtilis fermentation liquor 12-14, bacillus amyloliquefaciens zymotic fluid
It is 13-16, wax printing fabric zymotic fluid 8-10, bacillus megaterium zymotic fluid 8-10, the lichen bacillus ferments liquid 6-8, more
Viscous fermentation of bacillus liquid 10-12.
3. microorganism composite pesticide according to claim 1, it is characterised in that：The microbial fermentation mixed liquor is according to quality
Part include bacillus thuringiensis zymotic fluid 33, bacillus subtilis fermentation liquor 13, bacillus amyloliquefaciens zymotic fluid 15, wax-like
Bacillus fermentation liquid 9, bacillus megaterium zymotic fluid 10, the lichen bacillus ferments liquid 8；Bacillus polymyxa zymotic fluid
12。
4. microorganism composite pesticide according to claim 1, it is characterised in that：The synergist includes amine ester according to mass parts
Fresh 35, calcium acetate 5, zinc sulfate 8, ammonium nitrate 13, chitosan 15, Tea Saponin 7, urea 17.
5. microorganism composite pesticide according to claim 1, it is characterised in that：In the bacillus thuringiensis zymotic fluid,
Bacillus subtilis fermentation liquor, bacillus amyloliquefaciens zymotic fluid, wax printing fabric zymotic fluid, the lichen bacillus ferments
Strain content is hundred million cfu/mL of 50-80 in liquid, bacillus polymyxa zymotic fluid.
6. microorganism composite pesticide according to claim 1, it is characterised in that：Strain in the bacillus megaterium zymotic fluid
Content is hundred million/g of 80-100.
7. microorganism composite pesticide according to claim 1, it is characterised in that：The preparation method of microorganism composite pesticide includes
Following steps：The microbial fermentation mixed liquor of mass parts is sucked by pressure difference in finished pot, is 120r/min's in rotating speed
Under the conditions of after rotation 20-30min, continue the addition of the synergist of mass parts to be stirred until homogeneous suspension.
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| CN201810256407.9A CN108513988A (en) | 2018-03-27 | 2018-03-27 | Microorganism composite pesticide |
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| CN113712077A (en) * | 2021-08-23 | 2021-11-30 | 南京工业大学 | Fruit preservative compounded by using bacillus fermentation product and application thereof |
| CN115287231A (en) * | 2022-08-10 | 2022-11-04 | 河北省科学院生物研究所 | Bacillus polymyxa ZLP-61 and application thereof |
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Application publication date: 20180911 |