CN104642391A

CN104642391A - Microbial agent for preventing and treating vegetable diseases and preparation method thereof

Info

Publication number: CN104642391A
Application number: CN201310575364.8A
Authority: CN
Inventors: 孙义
Original assignee: LEADING GREEN BIOLOGICAL ZHENJIANG Co Ltd
Current assignee: LEADING GREEN BIOLOGICAL ZHENJIANG Co Ltd
Priority date: 2013-11-18
Filing date: 2013-11-18
Publication date: 2015-05-27

Abstract

The invention relates to a microbial agent for preventing and treating vegetable diseases and a preparation method thereof. The preparation method comprises the following steps of carrying out individual fermentation on bacillus subtilis, bacillus licheniformis and bacillus megaterium, processing the fermentation product to obtain powder, and preparing the microbial agent by blending according to a ratio. The microbial agent has effects of inhibiting harmful bacteria, improving stress resistance, improving a yield, improving quality and reducing farm chemical use.

Description

A kind of microbial bacterial agent preventing and treating vegetable disease and preparation method thereof

Technical field

The invention belongs to agriculture using microbe technical field.

Background technology

Using of chemical pesticide is the main method of preventing and treating vegetable disease.Use chemical pesticide in a large number for a long time, the series of problems such as the residual and environmental pollution of the pathogen resistance brought, agricultural product medicament, seriously constrain the sustainable development of agricultural, threaten the health of the mankind simultaneously.In recent years, the raising be familiar with food security along with people, also more and more higher to the requirement of food quality, it is imperative therefore to seek a kind of nuisanceless, free of contamination disease control new method.Measure of biotic control has the features such as safe, efficient, economic, free from environmental pollution because of it, has become the important prevention and controls of vegetable disease.

Summary of the invention

Technical problem

The object of this invention is to provide " a kind of microbial bacterial agent preventing and treating vegetable disease and preparation method thereof ", by bacillus subtilis, after bacillus licheniformis and bacillus megaterium are fermented separately, then pulvis is made, be re-dubbed microbial bacterial agent in proportion, this microbial inoculum has suppression harmful bacteria, strengthens resistance, reaches the use improving output, oil recovery enhancement, minimizing chemical pesticide.

Technical scheme

A kind of microbial bacterial agent preventing and treating vegetable disease of the present invention and preparation method thereof, its step is as follows:

(1) be inoculated in medium by the inoculum concentration of 2%-10% by the bacillus subtilis activated and carry out shaking table cultivation, this medium is: sucrose 15-20g/l, peptone 2-5g/l, sodium chloride 2-10g/l, magnesium sulfate 0.1-0.5g/l, zinc sulphate 0.01-0.1g/l, PH=7.0-7.2.Condition of culture is: 150-200r/min, temperature are 30-37 degree, throughput is 1:1; Bacillus subtilis viable bacteria pulvis is made after cultivating after 24-48 hour concentrated, drying;

(2) Bacillus licheniformis strain activated is inoculated in medium by the inoculum concentration of 2%-10% carries out shaking table cultivation, this medium is as follows: sucrose 10-20g/l, yeast extract 3-10g/l, potassium dihydrogen phosphate 3-10g/l, zinc sulphate 0.01-0.1g/l, sodium chloride 2-10g/l, magnesium sulfate 0.5-2g/l, to regulate after PH=6-8 121 degree of sterilizings 20 minutes with running water after dissolving; Condition of culture is: cultivation temperature is 30-37, and throughput is that 1:1 cultivation is concentrated after 36-72 hour, bacillus licheniformis viable bacteria pulvis is made in drying;

(3) the bacillus megaterium bacterial classification activated is inoculated in medium by the inoculum concentration of 2%-10% carries out shaking table cultivation, this medium is as follows: sucrose 10-20g/l, yeast extract 3-10g/l, potassium dihydrogen phosphate 3-10g/l, zinc sulphate 0.01-0.1g/l, sodium chloride 2-10g/l, magnesium sulfate 0.5-2g/l, to regulate after PH=6-8 121 degree of sterilizings 20 minutes with running water after dissolving; Condition of culture is: cultivation temperature is 30-37, and throughput is that 1:1 cultivation is concentrated after 36-72 hour, bacillus megaterium viable bacteria pulvis is made in drying;

(4) by above-mentioned bacillus subtilis viable bacteria pulvis, bacillus licheniformis viable bacteria pulvis, bacillus megaterium viable bacteria pulvis in (1-3): (1-3): the ratio mixing of (1-3) compositely obtains microorganism formulation.

Embodiment:

Embodiment

By the bacillus subtilis activated by 2% inoculum concentration be inoculated in medium and carry out shaking table cultivation, this medium is: sucrose 20g/l, peptone 5g/l, sodium chloride 10g/l, magnesium sulfate 0.3g/l, zinc sulphate 0.03g/l, PH=7.0; Condition of culture is: 180r/min, temperature are 37 degree, throughput is 1:1; Bacillus subtilis viable bacteria pulvis is made after cultivating after 24 hours concentrated, drying;

By the Bacillus licheniformis strain activated by 5% inoculum concentration be inoculated in medium and carry out shaking table cultivation, this medium is as follows: sucrose 15g/l, yeast extract 5g/l, potassium dihydrogen phosphate 3g/l, zinc sulphate 0.02g/l, sodium chloride 2g/l, magnesium sulfate 2g/l, to regulate after PH=6 121 degree of sterilizings 20 minutes with running water after dissolving; Condition of culture is: cultivation temperature is 37, and throughput is that bacillus licheniformis viable bacteria pulvis is made in concentrated after 1:1 cultivates 36 hours, drying;

By the bacillus megaterium bacterial classification activated by 6% inoculum concentration be inoculated in medium and carry out shaking table cultivation, this medium is as follows: sucrose 12g/l, yeast extract 4g/l, potassium dihydrogen phosphate 5g/l, zinc sulphate 0.04g/l, sodium chloride 5g/l, magnesium sulfate 0.5g/l, to regulate after PH=8 121 degree of sterilizings 20 minutes with running water after dissolving; Condition of culture is: cultivation temperature is 35 degree, and throughput is that bacillus megaterium viable bacteria pulvis is made in concentrated after 1:1 cultivates 48 hours, drying;

Above-mentioned bacillus subtilis viable bacteria pulvis, bacillus licheniformis viable bacteria pulvis, bacillus megaterium viable bacteria pulvis are obtained microorganism formulation in the ratio mixing of 1:1:1 is composite;

Mentioned microorganism preparation is used for tomato and cucumber carries out effect experimental:

(1) bring into use microorganism formulation at graw mold of tomato initial stage of origination, every mu of consumption 200-300 gram, once, use 3 times continuously, the average preventive effect of final control efficiency blade and fruit all reaches more than 82% in experiment per week;

(2) cucumber every mu consumption 300-400 gram, prevents and treats gray mold of cucumber by foliage spray, and after dispenser, the preventive effect of 96 hours is 76%.

Claims

1. prevent and treat a microbial bacterial agent for vegetable disease, it is characterized in that bacillus subtilis, bacillus licheniformis, bacillus megaterium 3 bacterial classifications to carry out composite forming.

2. microbial bacterial agent preventing and treating vegetable disease and preparation method thereof, is characterized in that following steps:

(1) be inoculated in medium by the inoculum concentration of 2%-10% by the bacillus subtilis activated and carry out shaking table cultivation, this medium is: sucrose 15-20g/l, peptone 2-5g/l, sodium chloride 2-10g/l, magnesium sulfate 0.1-0.5g/l, zinc sulphate 0.01-0.1g/l, PH=7.0-7.2;

Condition of culture is: 150-200r/min, temperature are 30-37 degree, throughput is 1:1; Bacillus subtilis viable bacteria pulvis is made after cultivating after 24-48 hour concentrated, drying;