CN102060578A - Method for producing microecological foliar fertilizer by utilizing probiotics and microalgae - Google Patents

Abstract

The invention belongs to the technical field of biology and relates to a method for producing a microecological foliar fertilizer by utilizing probiotics and microalgae. The method comprises the following steps of: firstly, culturing to obtain liquid culture prepared by bacillus, lactic acid bacteria and rhodopseudomonas palustris with the cell concentration being 10 billion/ml and saccharomycetes and chlorella with the cell concentration being 2 billion/ml respectively, then preparing liquid culture with four types of probiotics and one type of chlorella, and mixing the liquid cultures according to respective volume ratio to obtain the liquid microecological foliar fertilizer with the total cell concentration being more than 5 billion/ml. The method has the advantages that the used strains and chlorella are all probiotics which can be eaten by human, and are safe and non-toxic; the microecological foliar fertilizer integrates the advantages of promoting the plant growth of the probiotics, the chlorella and metabolites thereof; and the microecological foliar fertilizer is diluted with water by 100 times every 10 days and is sprayed on plant leaves, and the plant yield is increased by more than 30% in 60 days. The method has an important application value in aspect of improving the yield and the quality of crops such as grain and the like.

Description

A kind of method of utilizing probiotic bacterium and little algae to produce little ecological foliage fertilizer

Technical field

The invention belongs to biological technical field, a kind of method of utilizing probiotic bacterium and little algae to produce little ecological foliage fertilizer particularly is provided, have very important using value and prospect aspect the nontoxic and composite highly effective leaf leaven in production safety.

Background technology

Foliage fertilizer refers to impose on the fertilizer that plant leaf also can be absorbed utilization, because foliage fertilizer can directly enter in the plant materials from leaf portion, participate in metabolism and the organic building-up process of crop, can in time remedy the required nutritive substance of crop in the time of therefore a little less than crop growth later stage root system receptivity.Rationally use the yield and quality that foliage fertilizer can improve crop.As the foliage fertilizer of emerging fertilizer representative, its research and application more and more have been subjected to people's generally attention.

Foliage fertilizer originates from France the earliest, from botanist Grisb in 1884 FeSO ₄Solution has spread upon on the jaundice grape leave in order to since correcting the yellowtop that causes because of iron deficiency, and foliage fertilizer is used in production practice and research has had significant progress.One of agrochemistry originator's Byccehro thinks, leaf when absorbing water droplet can as root absorption of nutrient ingredients in plant.Studies show that further plant can be through leaf portion assimilation magnesium and potassium and trace element.China peasant of Zhejiang Province begins the rice application foliage fertilizer before more than 200 years, has opened up the precedent of China's application foliage fertilizer.Middle 1980s, China began to research and produce the novel foliage fertilizer that different sorts contains plant-growth regulator or farm chemical ingredients, made the function variation more of foliage fertilizer.After nineteen nineties, some novel foliage fertilizers application effect in the plantation of cash crop such as watermelon, citrus and grape is very remarkable.Along with foliage fertilizer research and going deep into of using, the type of foliage fertilizer is enriched constantly, and kind constantly increases, and from initial single type foliage fertilizer, develops into present multiclass composite foliage fertilizer.The present nutritious type foliage fertilizer of the main type of foliage fertilizer, adjustment type foliage fertilizer, microbiocidal foliage fertilizer, single type microelement foliage fertilizer and composite foliage fertilizer etc., wherein microorganism foliage fertilizer is a dark horse, and becomes the outstanding person in the foliage fertilizer.Microorganism foliage fertilizer contains microbial cells and metabolite thereof, can stimulate plant growth, promotes crop metabolism, alleviates and prevent the generation of disease and pest.But do not report aspect little ecological foliage fertilizer adopting probiotic bacterium and little algae to make up to produce.

Animal microecological formulation refers to use little ecological principle will be from isolating probiotic bacterium in the animal body, the active bacteria formulation of cultivating through special process.Probiotic bacterium (probiotics) speech is the earliest in order to describe " by a kind of material that can stimulate another kind of microorganism growth of microorganism secretion ".Probiotic bacterium was defined as " being of value to animal host's viable bacteria feed additive by improving the intestinal microflora balance " in 1989.The latest definition that European food in 2002 and feed association have provided probiotic bacterium for " probiotic bacterium is the microorganism that lives, by take in can to the host produce one or more special and through proving the functional health benefit ".Because probiotics can promote growth of animal, improve meat, egg and milk quality and be expected to replace microbiotic, become the focus and the forward position of domestic and international research and application, but adopted probiotic bacterium rare as the research report in leaf leaven field.

Little Trentepohlia is the simplest photosynthesis organism in lower plant.Chlorella in little algae (Chlorella) is a kind of unicell green alga, contain multiple nutrients and activeconstituentss such as rich in protein, carotenoid, polysaccharide, VITAMIN and chlorella growth factor, that chlorella glycoprotein has is antitumor, improve the activity of immunizing power and anti-virus infection, is the human health food of high value.Chlorella growth factor has the activity that strengthens cellular metabolism, delaying cell aging, has important pharmaceutical use.But adopt chlorella not report as the foliage fertilizer aspect.

Summary of the invention

In order to address the above problem, the purpose of this invention is to provide and a kind ofly adopt multiple probiotic bacterium and chlorella to produce a kind of little ecological foliage fertilizer that can promote the plant-growth safety non-toxic, by being sprayed on the plant leaf surface, with the method for the little ecological foliage fertilizer of production that reaches the purpose that improves plant biomass and quality.

Technical scheme of the present invention: a kind of method of utilizing probiotic bacterium and little algae to produce little ecological foliage fertilizer, it is characterized in that, comprise that specifically following work step is rapid:

At first, cultivate the acquisition cell concn respectively separately and reach the above red false unit cell in genus bacillus, milk-acid bacteria and marsh of 10,000,000,000/ml, and cell concn reaches 2,000,000,000/ml above chlorella and saccharomycetic liquid culture;

Secondly, to account for volume percent respectively and be 10-20% genus bacillus culture, to account for culture that volume percent is the milk-acid bacteria of 10-20%, account for culture that volume percent is the Rhodopseudomonas palustris of 10-30%, account for volume percent be the yeast culture of 10-20% and to account for volume percent be that the liquid culture of the chlorella of 10-20% carries out uniform mixing, can obtain total cell concn and reach the little ecological foliage fertilizer of the above liquid high value of 5,000,000,000/ml.

The invention has the advantages that: 1) used bacterial classification and chlorella all are human edible probiotic bacterium and heath food, safety non-toxic.2) little ecological foliage fertilizer collection probiotic bacterium and chlorella and meta-bolites thereof promote the advantage of plant-growth to gather together.3) after being sprayed on 100 times of little ecological foliage fertilizer dilutions on the plant leaf surface in 10 days, can improving plant biomass about 60 days and reach more than 30%.Have important use is worth in fields such as Organic food, vegetables, flowers, fruit, tealeaves and leaf tobacco production.

Description of drawings

Fig. 1 obtains cell concn figure in different probiotic bacteriums and the chlorella culture respectively in the preparation process of specific embodiments of the invention 1.

Fig. 2 is the total cell concn figure that obtains little ecological foliage fertilizer in the preparation process of specific embodiments of the invention 1.

Fig. 3 is the figure that the little ecological foliage fertilizer of specific embodiments of the invention 1 production improves the Chinese cabbage speed of growth.

Embodiment

Below in conjunction with specific embodiment technical scheme of the present invention is described further.

Principle of the present invention is: probiotic culture comprises mycetocyte and metabolite thereof, possesses stimulating plant growth and metabolism, alleviates and prevents disease and pest generation function.Various active compositions such as chlorella culture rich in proteins, carotenoid, polysaccharide, VITAMIN, chlorella growth factor and meta-bolites also have the effect that promotes plant-growth.The little ecological foliage fertilizer of producing after probiotic culture and chlorella culture mixed according to a certain percentage, the advantage that algae and bacterium and meta-bolites thereof is improved plant-growth combines, and can increase substantially the speed of growth and the resistance against diseases of plant.

Embodiment 1

Fermentation culture obtains cell concn and is respectively 12,900,000,000/ml bacillus natto at first separately, 11,800,000,000/ml lactobacillus, 10,600,000,000/ml Rhodopseudomonas palustris, the liquid culture of 2,500,000,000/ml S. cervisiae and 2,200,000,000/ml chlorella USTB-01, as shown in Figure 1, with cell concn respectively 12,900,000,000/ml bacillus natto 100ml, 11,800,000,000/ml lactobacillus 100ml, 10,600,000,000/ml Rhodopseudomonas palustris 100ml, 2,500,000,000/ml S. cervisiae 100ml and 2,200,000,000/ml chlorella USTB-01100ml mix, having obtained total cell concn is the little ecological foliage fertilizer of 8,000,000,000/ml, as shown in Figure 2.With the little ecological foliage fertilizer of producing of forming by 4 kinds of probiotic bacteriums and chlorella culture, after adopting water to dilute 100 times, the method that evenly was sprayed on the Chinese cabbage blade face 1 time in 10 days according to every interval is used, treat that Chinese cabbage growth got respectively to spray in 60 days and use and do not spray each 10 Chinese cabbage of using little ecological foliage fertilizer and weigh, the result shows that spraying average every the weight of the Chinese cabbage of using little ecological foliage fertilizer is 5.6 kilograms, do not use average every Chinese cabbage weight on little ecological blade face only to be 4.3 kilograms and spray, as shown in Figure 3, use the Chinese cabbage increase output of little ecological foliage fertilizer to reach 30.2%.

Embodiment 2

Fermentation culture obtains cell concn and is respectively 12,000,000,000/ml bacillus natto, 12,800,000,000/ml lactobacillus, 10,200,000,000/ml Rhodopseudomonas palustris, 2,600,000,000/ml S. cervisiae and 2,500,000,000/ml chlorella USTB-01 totally 5 kinds of liquid cultures at first separately, mix according to the ratio that respectively accounts for total volume percent 10%, 20%, 30%, 20% and 20% respectively, having obtained total cell concn is the little ecological foliage fertilizer of 78.4 hundred million/ml.

Embodiment 3

Fermentation culture obtains cell concn and is respectively 13,000,000,000/ml bacillus natto, 12,000,000,000/ml lactobacillus, 10,500,000,000/ml Rhodopseudomonas palustris, 2,300,000,000/ml S. cervisiae and 2,500,000,000/ml chlorella USTB-01 totally 5 kinds of liquid cultures at first separately, mix according to the ratio that respectively accounts for total volume percent 20%, 20%, 30%, 10% and 20% respectively, having obtained total cell concn is the little ecological foliage fertilizer of 88.8 hundred million/ml.

Claims (1)

1. a method of utilizing probiotic bacterium and little algae to produce little ecological foliage fertilizer is characterized in that, comprises that specifically following work step is rapid:

At first, cultivate the acquisition cell concn respectively separately and reach the above red false unit cell in genus bacillus, milk-acid bacteria and marsh of 10,000,000,000/ml, and cell concn reaches 2,000,000,000/ml above chlorella and saccharomycetic liquid culture;

Secondly, to account for volume percent respectively and be 10-20% genus bacillus culture, to account for culture that volume percent is the milk-acid bacteria of 10-20%, account for culture that volume percent is the Rhodopseudomonas palustris of 10-30%, account for volume percent be the yeast culture of 10-20% and to account for volume percent be that the liquid culture of the chlorella of 10-20% carries out uniform mixing, can obtain total cell concn and reach the little ecological foliage fertilizer of the above liquid high value of 5,000,000,000/ml.

Images