CN103598012A - Ganoderma lucidum mycelium and preparation method thereof - Google Patents
Ganoderma lucidum mycelium and preparation method thereof Download PDFInfo
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- CN103598012A CN103598012A CN201310573713.2A CN201310573713A CN103598012A CN 103598012 A CN103598012 A CN 103598012A CN 201310573713 A CN201310573713 A CN 201310573713A CN 103598012 A CN103598012 A CN 103598012A
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Abstract
Disclosed are a ganoderma lucidum mycelium and a preparation method thereof. The ganoderma lucidum mycelium specifically comprises a culture medium composed of rice bran, wheat bran, corn flour and brown sugar. A ganoderma lucidum liquid strain is taken as a strain, an inoculation method and inoculation amount of the liquid strain are well grasped, and a high-quality ganoderma lucidum mycelium finished product is cultured by reasonable arrangement of the production environment. By implementation of the method, the problem that ganoderma lucidum fruiting bodies cannot be produced massively to further affect application of ganoderma lucidum medicine sources due to insufficient wild ganoderma lucidum resources and high artificial cultivation difficulty is solved. Meanwhile, the production and preparation technology of the final product, namely the ganoderma lucidum mycelium, has the advantages of short cultivation period, simplicity, low production cost, high production efficiency, high stability and the like. Besides, the final product, namely the ganoderma lucidum mycelium serving as an additive, is widely applied to the fields of pharmacy, biological health care products, special drinks, functional foods and the like, and is quite good in market prospect.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of ganoderma lucidum mycelium and preparation method thereof.
Technical background
Treasure in glossy ganoderma ,Shi motherland pools of traditional Chinese medicine, have the good reputation of " celestial grass ".Pharmacology and clinical research at all times all proves, the effect that glossy ganoderma truly has and prevents and cures diseases, promotes longevity.The < < Sheng Nong's herbal classic > > of the Eastern Han Dynasty, the < < Compendium of Materia Medica > > of the famous medical scholar's Li Shizhen (1518-1593 A.D.) of the Ming Dynasty, have in detail and very sure record the effect of glossy ganoderma.Modern pharmacology and clinical practice have further confirmed the pharmacological action of glossy ganoderma, and confirm that GL-B is that glossy ganoderma is strengthened the body resistance to consolidate the constitution, strengthening by means of tonics, the main component of promoting longevity.Now, glossy ganoderma is formally recorded by NF as medicine, and it is again the new resource food of state approval simultaneously, has no side effect, can medicine-food two-purpose.
Scientific research shows, the pharmaceutical component of glossy ganoderma is very abundant, wherein effective ingredient can be divided into ten large classes, comprise GL-B, glossy ganoderma polypeptide, triterpenes, 16 seed amino acids (wherein containing seven kinds of essential amino acids), protein, steroid class, mannitol, coumarin glycosides, alkaloid, organic acid (main containing fumaric acid), and micro-Ge, P, Fe, Ca, Mn, Zn, etc.Glossy ganoderma has dual regulation to human body, and the kind of curing the disease relates to each systems such as cardiovascular and cerebrovascular, digestion, nerve, internal secretion, breathing, motion, especially very remarkable to the preventive and therapeutic effect of tumour, hepatic disease, insomnia and aging.
Along with the medical value of glossy ganoderma is extensively familiar with by people, the Wild ganoderma resource of modern remote, thickly forested mountains is also in continuous minimizing.At present, the ganoderma lucidum product on market adopts artificial cultivation mostly, follow the natural variation that has, but its curative effect is identical by its product form of ganoderma lucidum fruitbody of artificial cultivation.But tame shortcoming is exactly inevitably will use agricultural chemicals in the course of cultivation and chemical method is cultivated bacterial classification, will use agricultural chemicals and control control of germ insect pest etc. in cultivation management process, to ganoderma lucidum fruitbody, brings substantial chemical contamination.Yet chemical contamination is the source of mankind's various diseases often.In addition, the biological transformation ratio of glossy ganoderma is always all very low, but the cycle of Ganoderma Lucidum is long, adds that production technology and management process are complicated and loaded down with trivial detailsly makes production cost cross height to cause benefit low.
Along with technical research is constantly goed deep into, by biological submerged fermentation technology, obtained brand-new ganoderma lucidum product---ganoderma lucidum mycelium.Ganoderma lucidum mycelium is to be sprouted and formed mycelia by Reishi sporule, and ganoderma lucidum fruitbody is constantly to decompose by mycelia, absorbs nutrient, and at suitable illumination, air, other condition such as temperature forms while coordinating.After fruit body maturation, launch conidial powder, also just formed the history of life of glossy ganoderma, the needed constitute of ganoderma lucidum fruitbody, has nutriment over half to derive from the storage nutrient in mycelium.Through relevant departments, detect: ganoderma lucidum powder has the identical active ingredient of glossy ganoderma, wherein the content of thick polysaccharide and GL-B and protein is far away higher than natural glossy ganoderma.The mycelium product developing is similar for the action effects such as insomnia forgetfulness, in poor health, neurasthenia and ganoderma spove powder; For treatment hypertension, coronary heart disease, diabetes, cancer, have remarkable result, so ganoderma lucidum mycelium is by named as " good health and a long life food ".
Summary of the invention
Object of the present invention is just to provide brand-new a kind of ganoderma lucidum mycelium and preparation method thereof.Specifically comprise: the medium being formed by rice bran, wheat bran, corn flour, brown sugar.With Liquid Strain of Ganoderma Lucidum, as bacterial classification, get hold of inoculation method and the inoculum concentration of liquid spawn, by the environment of reasonably arranging production, turn out the ganoderma lucidum mycelium finished product of high-quality.By implementing the present invention, effectively solved because the deficiency of Wild ganoderma resource and artificial cultivation difficulty cause greatly ganoderma lucidum fruitbody in a large number output then affect the application problem in glossy ganoderma medicine source.Meanwhile, final products---ganoderma lucidum mycelium, its process for producing, has the features such as cultivation period is short, process for producing simple, the production cost production efficiency low, product of product is high and stable.In addition, by implementing the present invention, final products---ganoderma lucidum mycelium, is widely used in the fields such as pharmacy, biological health-care product, characteristic drink, functional food as a kind of additive, and market prospects are very good.
The present invention is achieved through the following technical solutions:
A kind of ganoderma lucidum mycelium and preparation method thereof, comprises the steps: in technical scheme
1, prepare medium: the raw material of medium is by 46% rice bran, 42% wheat bran, 9% corn flour, 3% brown sugar forms, first by rice bran, wheat bran, corn flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50~55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5~6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method.
2, inoculation: under gnotobasis, cultured Liquid Strain of Ganoderma Lucidum is transferred in culture matrix.
3, cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4~5 days under 26~29 ℃, the relative moisture environment that is 60~65% in temperature, and medium covers with Ganoderma lucidum mycelium.
4, finished product: when Ganoderma lucidum mycelium covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
The present invention both had the following advantages:
1, ganoderma lucidum mycelium its preparation method technique proposed by the invention is simple, easily implements.
2, by implementing the present invention, effectively solved because the deficiency of Wild ganoderma resource and artificial cultivation difficulty cause greatly ganoderma lucidum fruitbody in a large number output then affect the application problem in glossy ganoderma medicine source.
3, by implementing the present invention, final products---ganoderma lucidum mycelium, its process for producing, has the features such as cultivation period is short, process for producing simple, the production cost production efficiency low, product of product is high and stable.
4, by implementing the present invention, final products---ganoderma lucidum mycelium, is widely used in the fields such as pharmacy, biological health-care product, characteristic drink, functional food as a kind of additive, and market prospects are good, remarkable benefit.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
A kind of ganoderma lucidum mycelium and preparation method thereof, specific implementation method is as follows:
1, prepare medium: the raw material of medium is by 46% rice bran, 42% wheat bran, 9% corn flour, 3% brown sugar forms, first by rice bran, wheat bran, corn flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50~55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5~6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method.
2, inoculation: under gnotobasis, cultured Liquid Strain of Ganoderma Lucidum is transferred in culture matrix.
3, cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4~5 days under 26~29 ℃, the relative moisture environment that is 60~65% in temperature, and medium covers with Ganoderma lucidum mycelium.
4, finished product: when Ganoderma lucidum mycelium covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
Claims (1)
1. ganoderma lucidum mycelium and preparation method thereof, it is to comprise the steps:
(1) prepare medium: the raw material of medium is by 46% rice bran, 42% wheat bran, 9% corn flour, 3% brown sugar forms, first by rice bran, wheat bran, corn flour mix and stir and mixed material, brown sugar is diluted in appropriate clear water again, then mixed material and syrup are mixed and stirred, and the water content of mixed material is adjusted into 50~55% must produces with raw material, then raw material is sub-packed in culture vessel, thickness when raw material packs culture vessel into is 5~6 centimetres, finally the culture vessel that raw material is housed is sealed and sterilization according to a conventional method,
(2) inoculation: under gnotobasis, cultured Liquid Strain of Ganoderma Lucidum is transferred in culture matrix;
(3) cultural hypha: the culture vessel connecting kind being moved on to culturing room's lucifuge and cultivate, is to cultivate 4~5 days under 26~29 ℃, the relative moisture environment that is 60~65% in temperature, and medium covers with Ganoderma lucidum mycelium;
(4) finished product: when Ganoderma lucidum mycelium covers with medium, medium is obtained to finished product mycelium by low temperature drying to moisture content in medium lower than 8%.
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Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104082033A (en) * | 2014-06-10 | 2014-10-08 | 信芝(厦门)生物科技有限公司 | Ganoderma lucidum planting method |
CN105053991A (en) * | 2015-08-18 | 2015-11-18 | 雷色香 | Lentinus edodes mycelium powder |
CN105123270A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | Basswood ganoderma lucidum planting method |
CN105586267A (en) * | 2015-12-25 | 2016-05-18 | 江苏大学 | Ganoderma lucidum mutant strain for production of Ganoderma lucidum mycelia |
CN105907651A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Preparation of ganoderma lucidum mycelia by taking dendrobium candidum as culture medium |
CN106036331A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Lucid ganoderma biological beverage prepared by taking horseradish tree extracting solution as carrier |
CN106047722A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Ganodorma lucidum mycelia with dampness dispelling and weight losing functions |
CN106072088A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | Thallus Laminariae (Thallus Eckloniae) Ganoderma mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
CN106070852A (en) * | 2016-06-29 | 2016-11-09 | 黄秀英 | With Folium hydrangeae strigosae extracting solution for a kind of Ganoderma biological drink of carrier preparation |
CN106085879A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | One medical material is the comprehensive mycelium of culture medium culturing Ganoderma |
CN106107942A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of breeding method of Moringa Auricularia mycelium |
CN106119129A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | With pitaya peel for a kind of Ganoderma mycelium of culture medium preparation |
CN106119126A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of Moringa Ganoderma mycelium |
CN106967619A (en) * | 2017-04-25 | 2017-07-21 | 井冈山市益生缘灵芝生态园有限公司 | A kind of preparation method of Medium for Ganoderma lucidum and ganoderma lucidum mycelium |
CN107095300A (en) * | 2017-03-08 | 2017-08-29 | 辽宁山水源植物科技有限公司 | A kind of biological nutrition compound composition of auxiliary treatment diabetes |
CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
CN108310020A (en) * | 2018-02-06 | 2018-07-24 | 青岛润达生物科技有限公司 | A kind of preparation method of ganoderma lucidum medicine matter mycelia |
CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
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2013
- 2013-11-16 CN CN201310573713.2A patent/CN103598012A/en active Pending
Cited By (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104082033A (en) * | 2014-06-10 | 2014-10-08 | 信芝(厦门)生物科技有限公司 | Ganoderma lucidum planting method |
CN105053991A (en) * | 2015-08-18 | 2015-11-18 | 雷色香 | Lentinus edodes mycelium powder |
CN105123270A (en) * | 2015-08-31 | 2015-12-09 | 佛山市高明区更合镇鹏鹄食用菌专业合作社 | Basswood ganoderma lucidum planting method |
CN105586267A (en) * | 2015-12-25 | 2016-05-18 | 江苏大学 | Ganoderma lucidum mutant strain for production of Ganoderma lucidum mycelia |
CN105586267B (en) * | 2015-12-25 | 2018-11-06 | 江苏大学 | Produce the ganoderma lucidum mutagenic strain of ganoderma lucidum mycelium |
CN106119126A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of Moringa Ganoderma mycelium |
CN106036331A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Lucid ganoderma biological beverage prepared by taking horseradish tree extracting solution as carrier |
CN106072088A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | Thallus Laminariae (Thallus Eckloniae) Ganoderma mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) |
CN105907651A (en) * | 2016-06-28 | 2016-08-31 | 黄秀英 | Preparation of ganoderma lucidum mycelia by taking dendrobium candidum as culture medium |
CN106085879A (en) * | 2016-06-28 | 2016-11-09 | 黄秀英 | One medical material is the comprehensive mycelium of culture medium culturing Ganoderma |
CN106107942A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of breeding method of Moringa Auricularia mycelium |
CN106119129A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | With pitaya peel for a kind of Ganoderma mycelium of culture medium preparation |
CN106047722A (en) * | 2016-06-28 | 2016-10-26 | 黄秀英 | Ganodorma lucidum mycelia with dampness dispelling and weight losing functions |
CN106070852A (en) * | 2016-06-29 | 2016-11-09 | 黄秀英 | With Folium hydrangeae strigosae extracting solution for a kind of Ganoderma biological drink of carrier preparation |
CN107095300A (en) * | 2017-03-08 | 2017-08-29 | 辽宁山水源植物科技有限公司 | A kind of biological nutrition compound composition of auxiliary treatment diabetes |
CN106967619A (en) * | 2017-04-25 | 2017-07-21 | 井冈山市益生缘灵芝生态园有限公司 | A kind of preparation method of Medium for Ganoderma lucidum and ganoderma lucidum mycelium |
CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
CN108310020A (en) * | 2018-02-06 | 2018-07-24 | 青岛润达生物科技有限公司 | A kind of preparation method of ganoderma lucidum medicine matter mycelia |
CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
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