CN105907651A - Preparation of ganoderma lucidum mycelia by taking dendrobium candidum as culture medium - Google Patents
Preparation of ganoderma lucidum mycelia by taking dendrobium candidum as culture medium Download PDFInfo
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- CN105907651A CN105907651A CN201610482193.8A CN201610482193A CN105907651A CN 105907651 A CN105907651 A CN 105907651A CN 201610482193 A CN201610482193 A CN 201610482193A CN 105907651 A CN105907651 A CN 105907651A
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- culture medium
- herba dendrobii
- mycelia
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- ganoderma lucidum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to preparation of ganoderma lucidum mycelia by taking dendrobium candidum as a culture medium. Stems of dendrobium candidum are taken as a main raw material for producing the culture medium, the culture medium is sterilized and then ganoderma lucidum cultures are inoculated, then cultivation is carried out until mycelia overgrow the culture medium, then the culture medium with the mycelia is dried in the sun, and finally smashing is carried out, so that dendrobium candidum ganoderma lucidum mycelia are obtained. By implementing the invention, dendrobium candidum and ganoderma lucidum are combined into another characteristic combination, namely the dendrobium candidum ganoderma lucidum mycelia, by adopting biotechnology, and nutrients and effects of the dendrobium candidum and ganoderma lucidum are integrated, so that nutrients, functions and effects can be more outstanding, and health, disease prevention and disease treatment effects are more obvious; meanwhile, the production preparation technology of the dendrobium candidum ganoderma lucidum mycelia has the characteristics of short cultivation period, simple productive preparation technology, high production efficiency and stability.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to one and prepare Ganoderma mycelium with Herba Dendrobii for culture medium.
Background technology
Herba Dendrobii, has another name called: Herba hedyotis costatae, Yunnan iron sheet.Belong to micro-specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family.Stem is upright, cylindrical, long 9-35 centimetre, thick 2-4 millimeter, and sepal and petal yellow green are the most similar, long round shape lanceolar, are about 1.8cm, wide 4-5mm, the month at florescence 3-6.It is distributed mainly on the ground such as China Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the YIN-tonifying drug in tonic.
Herba Dendrobii is one of most precious Chinese crude drug of China, it is classified as " China's nine big Herba mesonae chinensis " (Herba Dendrobii, Herba Saussureae Involueratae, three double Radix Ginsengs by honor, 120 years Radix Polygoni Multiflori, the Poria of cycle of sixty years, Herba Cistanches, remote mountains Ganoderma, seabed Margarita, Cordyceps) first of, have the laudatory title of " gold in medicine ".
The application of Herba Dendrobii, the most on the books in the Shennong's Herbal in Eastern Han Dynasty's last years of a dynasty or reign, the Ancient Times in China multi-section medical literature such as Compendium of Material Medica, " Chinese medicine dictionary " all describes the medicinal function of Herba Dendrobii.
Learning from relevant document, its ten main large effects of Herba Dendrobii have:
(1) nourishing Tianjin: " China's pharmacy dictionary " claims Herba Dendrobii " specially to grow the gas-liquid of lung stomach, gas-liquid is rushed prosperous, and kidney water is spontaneous ", is good at YIN nourishing and the production of body fluid promoting, treats all diseases of Yin and fluid deficiency.
(2) health invigorating: Herba Dendrobii has the function of nourishing YIN and benefiting blood.Qing Dynasty's " property of medicine opinion " says: essence, nourishing stomach-YIN, physical strength profiting are amassed in Herba Dendrobii energy the kidney invigorating.Containing abundant polysaccharose substance, there is the effect strengthening immunologic function.
(3) strengthen the spleen and stomach: Herba Dendrobii is reinforcing stomach reg fluid medicine.In Shennong's Herbal, " book on Chinese herbal medicine is the newest " the most on the books, appellation " medicine for enterogastritis ", is the common drug that goes up painful for the treatment of gastric abscess, upper abdomen.Modern medical experiment confirms: Herba Dendrobii has good inhibiting effect to pathogenic bacterium helicobacter pylori common in the spleen-stomach disease, contributes to treating the disease of the Helicobacter pyloris such as atrophic gastritis, superficial gastritis, duodenal ulcer.
(4) hepatoprotective function of gallbladder promoting: Herba Dendrobii has preferable choleretic effect.Ancient Chinese medicine doctor all thinks that " Herba Dendrobii " has the effect of nourishing hepatic yin, is the key medicine treating various liver-gallbladder disease, can be used for treating the liver and gall diseases such as hepatitis, cholecystitis, cholelithiasis.
(5) strong muscle blood fat reducing: after people enters the middle age, cloudy Tianjin starts weak, and muscles and bones function is faded, Herba Dendrobii can vital essence replenishing, lubricating joint, thus reach strengthening the tendons and bones, fluent joint, strengthen antiheumatic effect.Modern pharmacological research also shows: Herba Dendrobii can improve stress ability, has good resisting fatigue, resisting oxygen lack；Circulation, expansion blood vessel can be promoted, reduce cholesterolemia and triglyceride.
(6) blood glucose is reduced: Herba Dendrobii function replenishing YIN and removing heat is moisturized, and treats the medicine dedicated of diabetes the most exactly.Clinical research shows, Herba Dendrobii is possible not only to strengthen insulin active, can significantly reduce blood sugar level simultaneously, make blood sugar recovery normal.
(6) suppression tumor: Herba Dendrobii has killing action to some cell of the malignant tumor such as pulmonary carcinoma, ovarian cancer and promyelocytic leukemia, has stronger anti-tumor activity.It is clinically used for the auxiliary treatment of malignant tumor, the symptom of tumor patient can be improved, alleviate the side effect of Radiotherapy chemotherapy, enhancing immunity, improve life quality, extend life span.
(8) bright eyes: Herba Dendrobii has YIN nourishing and supports purpose function, by ancient Chinese medicine doctor as the good merchantable brand of eye-protecting.Modern pharmacology research confirms: Herba Dendrobii is to preventing and treating senile cataract and protecting juvenile's vision to have positive effect.
(9) skin is nourished: after human body enters the middle age, owing to internal cloudy liquid reduces day by day, thus accelerate skin aging, be allowed to blackening or crumple.The lymphatic temperament that Herba Dendrobii contains, has moist Nutrition to human body skin.
(10) life lengthening: Herba Dendrobii is classified as by Shennong's Herbal has the panacea that " make light of one's life by commiting suicide and prolong life " acts on.Modern pharmacology research confirms: Herba Dendrobii contains various trace elements, and the good health and a long life for human body have close relationship, to the anti-aging effects of human body than general medicine more extensively, more comprehensively.
Summary of the invention
It is an object of the invention to provide brand-new one and prepare Ganoderma mycelium with Herba Dendrobii for culture medium.Specifically include: this mycelium is to be that primary raw material does culture medium by Herba Dendrobii stem section, Ganderma lucidum strain will be accessed after medium sterilization, then cultivate and cover with culture medium to mycelia, then the culture medium covering with mycelia is dried, finally pulverize to obtain Herba Dendrobii Ganoderma mycelium.
Described Ganoderma, is the treasure in motherland's pools of traditional Chinese medicine, have the good reputation of " Herba mesonae chinensis ".Pharmacology all proves with clinical research at all times, Ganoderma truly have prevent and cure diseases, effect of life lengthening.The Shennong's Herbal of the Eastern Han Dynasty, the Compendium of Material Medica of the Ming Dynasty's famous medicine scholar's Li Shizhen (1518-1593 A.D.), all effects to Ganoderma have record in detail and extremely certainly.Modern pharmacology and clinical practice further demonstrate that the pharmacological action of Ganoderma, and confirm that ganoderan is the main component of Ganoderma strengthening the body resistance, strengthening by means of tonics, life lengthening.Now, Ganoderma is the most formally recorded by state-promulgated pharmacopoeia as medicine, and it is again the new resource food of state approval simultaneously, has no side effect, can be with medicine-food two-purpose.
Scientific investigations showed that, the pharmaceutical component of Ganoderma is the abundantest, wherein active ingredient can be divided into ten big classes, including ganoderan, Ganoderma polypeptide, triterpenes, 16 kinds of aminoacid (wherein containing seven kinds of essential amino acids), protein, steroid, mannitol, coumarin glycosides, alkaloid, organic acid (main containing Fumaric acid), and trace element Ge, P, Fe, Ca, Mn, Zn, etc..Ganoderma has dual regulation to human body, kind of being cured the disease, relate to cardiovascular and cerebrovascular vessel, digestion, nerve, endocrine, breathe, each system such as motion, especially the most notable to the preventive and therapeutic effect of tumor, hepatic lesions, insomnia and aging.
Along with the medical value of Ganoderma is extensively recognized by people, the wild Ganoderma resource of modern remote, thickly forested mountains is also constantly reducing.At present, the ganoderma lucidum product on market uses artificial culture mostly, and by its product form of the Ganoderma sporophore of artificial culture with the natural variation that has, but its curative effect is identical.But tame shortcoming is exactly pesticide to be used with chemical method to cultivate strain, and during cultivation management, pesticide to be used controls control of pathogenic bacteria insect pest etc., brings substantial chemical contamination to Ganoderma sporophore.But, chemical contamination is often the source of the various disease of the mankind.It addition, the biological transformation ratio of Ganoderma is the lowest, but the cycle of Ganoderma Lucidum is long, add production technology and management process complicated and loaded down with trivial details make production cost cross height cause inefficiencies.
Along with technical research constantly deeply, brand-new ganoderma lucidum product Ganoderma mycelium is obtained by biological submerged fermentation technology.Ganoderma mycelium is to be sprouted by Ganoderma spore to form mycelia, and Ganoderma sporophore is constantly to be decomposed by mycelia, absorbs nutrient, in suitable illumination, and air, formation when other condition such as temperature coordinates.Launch spore powder after sporophore maturation, be also the formation of the life cycle of Ganoderma, the composition material required for Ganoderma sporophore, there is the nutrient substance of more than half to derive from the storage nutrient in mycelium.Detect through relevant departments: ganoderma lucidum powder has the effective ingredient that Ganoderma is identical, and wherein the content of crude polysaccharides and ganoderan and protein is significantly larger than natural glossy ganoderma.The mycelium product developed is similar for the action effects such as insomnia forgetfulness, physical weakness, neurasthenia and Ganoderma spore powder with cellular wall broken；For treatment hypertension, coronary heart disease, diabetes, cancer, there is remarkable result, so Ganoderma mycelium is by named as " good health and a long life food ".
Producing Ganoderma mycelium by biological submerged fermentation technology, the features such as it is with short production cycle, technique is simple, production efficiency is high, stable, are the main development directions of artificial culture ganoderma lucidum product.The by-product of the current raw material main food produced used by Ganoderma mycelium or plant amylum, rice meal, Semen Maydis powder, analysis for soybean powder etc., and to add a little mineral element on the basis of above-mentioned raw materials be carbon and nitrogen sources, mycelium cultivate after again by drying and being ground into powder.Mycelium can have been run off by its nutritional labeling after drying.In addition to function that the mycelium product additionally produced by such technique has except product itself, effect, the poor morphology of its product formation, mouthfeel are the best, even without any taste, it is impossible to attract much attention, so product promotion poor effect.
Specifically include following steps:
1. Herba Dendrobii collection and process: gather disease-free, be raw material without insect pest, non agricultural chemical residuum and fresh Herba Dendrobii, then the leaf of Herba Dendrobii is peeled off, again Herba Dendrobii is cut into the sections of 1-2 centimetre, finally Herba Dendrobii sections is dried to material moisture standby less than 8%.
2. preparation culture medium: culture medium is made up of the Herba Dendrobii sections of 90% and the Semen Maydis powder of 10%, first Herba Dendrobii sections is soaked among the G/W of 5%, pull out after persistently soaking 8-9 hour and drain away the water, it is 55-60% that Semen Maydis powder is also adjusted to moisture content with the G/W of 5%, mix Herba Dendrobii and Semen Maydis powder and stir to obtain mixed material the most again, mixed material is sub-packed in culture vessel, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally must produce after sterilizing and use culture medium.
3. inoculation: in an aseptic environment Ganderma lucidum strain is accessed production culture medium, when accessing solid Ganderma lucidum strain, the solid spawn of 7-8 point to be accessed, when accessing Liquid Strain of Ganoderma Lucidum, the 10-12% liquid spawn of culture medium weight to be accessed.
4. cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relative humidity be to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
5. dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%.
6. finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Herba Dendrobii Ganoderma mycelium.
The present invention both had the following advantages:
1, the method preparing Ganoderma mycelium for culture medium with Herba Dendrobii proposed by the invention is novel, and production technology is simply, easily implement.
2, by implementing the present invention, by biotechnology, Herba Dendrobii and Ganoderma are formed the coalition Herba Dendrobii Ganoderma mycelium of another characteristic, combine Herba Dendrobii and the nutrient of Ganoderma and action and efficacy simultaneously, make its nutrition, function and effect can be more prominent, and its health care and diseases prevention, effect of curing the disease are more notable.
3, by implementing the present invention, the process for producing of final products Herba Dendrobii Ganoderma mycelium, there is the features such as cultivation period is short, process for producing simple, the production efficiency of product is high and stablizes.
4, by implementing the present invention, the Herba Dendrobii Ganoderma mycelium of formation is of many uses, can be as being prepared as medicine, health product, the raw material of Speciality Foods additive.Also can be sub-packed in capsule and become a kind of characteristic and health care food medicine.Not only there is extraordinary market prospect, and the economic benefit produced also can highly significant.
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
Ganoderma mycelium is prepared for culture medium with Herba Dendrobii, technical scheme is: this mycelium is to be that primary raw material does culture medium by Herba Dendrobii stem section, Ganderma lucidum strain will be accessed after medium sterilization, then cultivate to mycelia and cover with culture medium, then the culture medium covering with mycelia is dried, finally pulverize to obtain Herba Dendrobii Ganoderma mycelium.
Detailed description of the invention is as follows:
1. Herba Dendrobii collection and process: gather disease-free, be raw material without insect pest, non agricultural chemical residuum and fresh Herba Dendrobii, then the leaf of Herba Dendrobii is peeled off, again Herba Dendrobii is cut into the sections of 1-2 centimetre, finally Herba Dendrobii sections is dried to material moisture standby less than 8%.
2. preparation culture medium: culture medium is made up of the Herba Dendrobii sections of 90% and the Semen Maydis powder of 10%, first Herba Dendrobii sections is soaked among the G/W of 5%, pull out after persistently soaking 8-9 hour and drain away the water, it is 55-60% that Semen Maydis powder is also adjusted to moisture content with the G/W of 5%, mix Herba Dendrobii and Semen Maydis powder and stir to obtain mixed material the most again, mixed material is sub-packed in culture vessel, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally must produce after sterilizing and use culture medium.
3. inoculation: in an aseptic environment Ganderma lucidum strain is accessed production culture medium, when accessing solid Ganderma lucidum strain, the solid spawn of 7-8 point to be accessed, when accessing Liquid Strain of Ganoderma Lucidum, the 10-12% liquid spawn of culture medium weight to be accessed.
4. cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relative humidity be to cultivate in the environment of 60～65% to mycelia to cover with culture medium.
5. dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%.
6. finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Herba Dendrobii Ganoderma mycelium.
Claims (1)
1. prepare Ganoderma mycelium with Herba Dendrobii for culture medium, it is characterized in that: this mycelium is to be that primary raw material does culture medium by Herba Dendrobii stem section, Ganderma lucidum strain will be accessed after medium sterilization, then cultivate to mycelia and cover with culture medium, then the culture medium covering with mycelia is dried, finally pulverize to obtain Herba Dendrobii Ganoderma mycelium；
Specifically include following steps:
(1) Herba Dendrobii collection and process: gather disease-free, be raw material without insect pest, non agricultural chemical residuum and fresh Herba Dendrobii, then the leaf of Herba Dendrobii is peeled off, again Herba Dendrobii is cut into the sections of 1-2 centimetre, finally Herba Dendrobii sections is dried to material moisture standby less than 8%；
(2) culture medium is prepared: culture medium is made up of the Herba Dendrobii sections of 90% and the Semen Maydis powder of 10%, first Herba Dendrobii sections is soaked among the G/W of 5%, pull out after persistently soaking 8-9 hour and drain away the water, it is 55-60% that Semen Maydis powder is also adjusted to moisture content with the G/W of 5%, mix Herba Dendrobii and Semen Maydis powder and stir to obtain mixed material the most again, mixed material is sub-packed in culture vessel, charge weight be mixed material thickness in culture vessel be 2-3cm, the most conventionally must produce after sterilizing and use culture medium；
(3) inoculation: in an aseptic environment Ganderma lucidum strain is accessed production culture medium, when accessing solid Ganderma lucidum strain, the solid spawn of 7-8 point to be accessed, when accessing Liquid Strain of Ganoderma Lucidum, the 10-12% liquid spawn of culture medium weight to be accessed；
(4) cultivation: the culture vessel connected kind is moved on to culturing room and carries out lucifuge cultivation, temperature be 20～23 DEG C, relative humidity be to cultivate in the environment of 60～65% to mycelia to cover with culture medium；
(5) dry: in the environment of temperature is less than 50 degree, the culture medium covering with mycelia is shone to material moisture less than 8%；
(6) finished product: the medium powder dried is broken to can cross 120-200 mesh and obtains finished product Herba Dendrobii Ganoderma mycelium.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103598012A (en) * | 2013-11-16 | 2014-02-26 | 覃秀花 | Ganoderma lucidum mycelium and preparation method thereof |
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- 2016-06-28 CN CN201610482193.8A patent/CN105907651A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103598012A (en) * | 2013-11-16 | 2014-02-26 | 覃秀花 | Ganoderma lucidum mycelium and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
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Application publication date: 20160831 |