CN105900696B - Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum - Google Patents
Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum Download PDFInfo
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- CN105900696B CN105900696B CN201610482734.7A CN201610482734A CN105900696B CN 105900696 B CN105900696 B CN 105900696B CN 201610482734 A CN201610482734 A CN 201610482734A CN 105900696 B CN105900696 B CN 105900696B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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Abstract
Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum; it is to do culture medium with dendrobium candidum stem section is primary raw material, Phellinus strain will be accessed after medium sterilization, then cultivate to mycelia and cover with culture medium; then the culture medium for covering with mycelia is dried, finally crushes to obtain dendrobium candidum phellinus igniarius mycelium.By implementing the present invention; dendrobium candidum and Phellinus are made up of biotechnology the combination of another characteristic --- dendrobium candidum phellinus igniarius mycelium; the nutrient and action and efficacy of dendrobium candidum and Phellinus are combined simultaneously; it protrude its nutrition, function and effect can more, and its health care and diseases prevention, effect of curing the disease are more significant.Meanwhile the process for producing of dendrobium candidum phellinus igniarius mycelium, have the characteristics that cultivation period is short, process for producing is simple, the high production efficiency of product and stablize.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to prepare phellinus liteus by culture medium of dendrobium candidum to one kind
Body.
Background technique
Dendrobium candidum, also known as: ribbed hedyotis herb, Yunnan iron sheet.Belong to micro- specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family.Stem is upright,
Cylinder, 9-35 centimetres long, 2-4 millimeters thick, sepal and petal yellow green, close similar, long round shape lanceolar is about 1.8cm, wide
4-5mm, the month at florescence 3-6.It is distributed mainly on the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic.
Dendrobium candidum is most precious one of the Chinese medicine in China, is classified as " Chinese nine big mesonas " (dendrobium candidum, Tianshan Mountains by honor
Saussurea involucrata, three double ginsengs, 120 Nian Shouwu, the Poria cocos of cycle of sixty years, desert cistanche, remote mountains ganoderma lucidum, seabed pearl, cordyceps sinensis) first of,
It is known as the laudatory title of " gold in medicine ".
The application of dendrobium candidum, early in the Shennong's Herbal in Eastern Han Dynasty's last years of a dynasty or reign just on the books, Compendium of Material Medica, " Chinese medicine
Dictionary " etc. the medicinal function of dendrobium candidum is all described in Ancient Times in China multi-section medical literature.
It is learned from related document, its main ten large effect of dendrobium candidum has:
(1) nourish yin jin: " Chinese pharmacy dictionary " claim dendrobium candidum " specially grow lung stomach gas-liquid, gas-liquid rush it is prosperous, kidney water from
It is raw ", it is good at nourishing Yin and promoting production of body fluid, treats all diseases of Yin and fluid deficiency.
(2) build up health: dendrobium candidum has the function of nourishing yin and nourishing blood.Qing Dynasty's " haigoushen " says: dendrobium candidum energy kidney tonifying
Product essence, nourishing stomach-YIN, benefiting energy.Polysaccharose substance rich in has the function of enhancing immune function.
(3) strengthen the spleen and stomach: dendrobium candidum is reinforcing stomach reg fluid medicine.It is on the books in Shennong's Herbal, " book on Chinese herbal medicine is new again ", claim
It calls " medicine for enterogastritis ", is the common drug treating epigastric pain, upper abdomen and going up painful.Modern medical experiment confirms: dendrobium candidum is to the spleen-stomach diseases
In common pathogenic bacteria helicobacter pylori have good inhibiting effect, help to treat atrophic gastritis, superficial gastritis, 12
The illness of the Helicobacter pyloris such as Duodenalulcer.
(4) protect liver cholagogue: dendrobium candidum has preferable choleretic effect.Ancient Chinese medicine doctor is all thought that " dendrobium candidum " has and is nourished
The effect of liver-yin is the key medicine for treating various liver and bladder diseases, can be used for treating the diseases in the liver and gallbladder such as hepatitis, cholecystitis, gall stone.
(5) strengthening tendons lipid-loweringing: people entered after the middle age, and yin jin starts weak, and muscles and bones function is faded, and dendrobium candidum can
Yin-fluid, lubricating joint are nourished, to achieve the effect that strengthening the muscles and bones, fluent joint, enhancing antirheumatic.Modern pharmacological research also table
Bright: dendrobium candidum can improve stress ability, have good antifatigue, resisting oxygen lack；It can promote circulation, expansion blood vessel, drop
Low cholesterolemia and triglyceride.
(6) reduce blood glucose: dendrobium candidum function nourishing yin and clearing heat are moisturized, and are since ancient times exactly the medicine dedicated for treating diabetes.
Clinical research shows that insulin active not only can be enhanced in dendrobium candidum, while can significantly reduce blood glucose level, makes blood sugar recovery
Normally.
(6) inhibit tumour: dendrobium candidum is certain to the malignant tumours such as lung cancer, oophoroma and promyelocytic leukemia
Cell has killing effect, has stronger anti-tumor activity.It is clinically used for the adjuvant treatment of malignant tumour, tumor patient can be improved
Symptom, mitigate the side effect of Radiotherapy chemotherapy, strengthen immunity improves life quality, extends life span.
(8) become clear eyes: dendrobium nobile has the function of the good merchantable brand that purpose is supported in enriching yin, by ancient Chinese medicine doctor as eye-protecting.It is modern
Pharmaceutical research confirms: dendrobium nobile has positive effect to prevention and treatment senile cataract and protection juvenile's eyesight.
(9) skin is nourished: after human body enters the middle age, since intracorporal yin-fluid is increasingly reduced, so that skin aging is accelerated,
It is allowed to blackening or crumple.The lymphatic temperament that dendrobium candidum contains has moist trophism to human skin.
(10) promote longevity: dendrobium candidum is classified as the panacea with " macrobiosis of making light of one's life by commiting suicide " effect by Shennong's Herbal.It is modern
Pharmaceutical research confirms: dendrobium candidum contains various trace elements, has close relationship for the good health and a long life of human body, to people
The anti-aging effects of body are more extensively, more comprehensively than general drug.
Summary of the invention
It is an object of the invention to provide completely new one kind to prepare phellinus igniarius mycelium by culture medium of dendrobium candidum.Specific packet
Include: the mycelium is to do culture medium with dendrobium candidum stem section is primary raw material, Phellinus strain will be accessed after medium sterilization, connect
Cultivate and to mycelia cover with culture medium, then the culture medium for covering with mycelia is dried, finally crushes to obtain dendrobium candidum phellinus liteus
Body.
The Phellinus is rare medicinal fungi, and fructification can be used as medicine.Its main parasitic is in mulberry, poplar, willow, birch, peach etc.
On the dry wood and standing tree of broad leaf tree and on trunk, major function is anticancer, and anti-fibrosis is anti-oxidant, treating cancer
Effective percentage is up to 90% or more.Phellinus, by the internationally recognized fungi for most anticancer function, is led in biological anticancer field in anticancer
Effective percentage ranks the first in domain, is international the world of medicine and health products trade antineoplastic product raw materials for production.The ancient Chinese medicine of this taste, name
Gas can not show a candle to cordyceps sinensis, but it the effect of it is but poor unlike cordyceps sinensis.Currently, it is internationally recognized biology Phellinus polysaccharide be from
Biologically active polysaccharose substance is extracted in the mycelium of Phellinus, fructification.Research confirms that Phellinus polysaccharide, which has, obviously exempts from
The effects of epidemic disease is adjusted, anticancer is antitumor, anti-oxidant, reduction blood glucose, protection liver.
And restricting current the factor that further develops and utilizes of Phellinus mainly have: (1) Phellinus wild resource is increasingly deficient,
Wild Phellinus resource is difficult to restore, and Phellinus fructification is caused not formed largely；(2) Phellinus is environmentally sensitive, and growth period is long, people
It is larger that work cultivates difficulty.In order to solve because the deficiency and artificial cultivation difficulty of wild Phellinus resource lead to greatly Phellinus fructification
Can not a large amount of outputs then influence the application problem of Phellinus medicine source；It is constantly studied by scientific research personnel, it is now deep by biology
Layer fermentation technique develops a kind of completely new Phellinus product --- phellinus igniarius mycelium.Studies have shown that phellinus igniarius mycelium it is effective at
Divide as fructification.Phellinus igniarius mycelium, with short production cycle, simple process, production are produced by biological submerged fermentation technology
The features such as high-efficient and stable is the main direction of development of artificial culture Phellinus product.At present used in production phellinus igniarius mycelium
Raw material is mainly grain fine fodder, such as corn flour, soybean powder, and it is carbon and nitrogen sources that a little inorganic salts elements are added on the basis of this raw material,
Then the phellinus igniarius mycelium of high-quality low-cost is turned out.
The present invention is achieved through the following technical solutions:
Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum, be in technical solution: the mycelium is to use dendrobium candidum
Stem section is primary raw material to do culture medium, Phellinus strain will be accessed after medium sterilization, then cultivates to mycelia and cover with culture medium,
Then the culture medium for covering with mycelia is dried, finally crushes to obtain dendrobium candidum phellinus igniarius mycelium.
Specifically comprise the following steps:
1. dendrobium candidum acquisition and processing: acquisition it is disease-free, without insect pest, non agricultural chemical residuum and fresh dendrobium candidum be original
Material, then peels the leaf of dendrobium candidum off, then dendrobium candidum is cut into 1-2 centimetres of segment, finally dendrobium candidum segment
It is dried to that material moisture is spare lower than 8%.
2. preparing culture medium: culture medium is made of 90% dendrobium candidum segment and 10% corn flour, first by iron sheet stone
Dry measure used in former times segment is soaked among 5% glucose water, is pulled out and is drained away the water after persistently impregnating 8-9 hour, corn flour also uses 5%
It is 55-60% that glucose water, which is adjusted to moisture content, and then dendrobium candidum and corn flour are mixed again and stir evenly to obtain mixing
Material is sub-packed in mixed material in culture vessel, and charge weight is that thickness of the mixed material in culture vessel is 2-3cm, most
Production culture medium is obtained after conventionally sterilizing afterwards.
3. inoculation: Phellinus strain being accessed production culture medium in an aseptic environment, when accessing solid Phellinus strain, to be connect
Enter the solid spawn of 7-8 point, when accessing Phellinus liquid spawn, to access the 10-12% liquid spawn of culture medium weight.
4. cultivation: the culture vessel that will be connected kind moves on to culturing room and carries out being protected from light culture, is 20~23 DEG C, relatively in temperature
Culture to mycelia covers with culture medium in the environment of humidity is 60~65%.
5. drying: shining the culture medium for covering with mycelia to material moisture lower than 8% in the environment of temperature is lower than 50 degree.
6. finished product: the medium powder dried, which is broken to, which can cross 120-200 mesh, obtains finished product dendrobium candidum phellinus igniarius mycelium.
The present invention both had the following advantages:
1, the method concept novelty proposed by the invention that phellinus igniarius mycelium is prepared using dendrobium candidum as culture medium, production technology
Simply, it is easy to implement.
2, by applying the present invention, dendrobium candidum and Phellinus are made up of biotechnology the combination of another characteristic
Body --- dendrobium candidum phellinus igniarius mycelium, while the nutrient and action and efficacy of dendrobium candidum and Phellinus are combined, make its nutrition, function
Can and effect can more protrude, and its health care and diseases prevention, effect of curing the disease are more significant.
3, by applying the present invention, final products --- the process for producing of dendrobium candidum phellinus igniarius mycelium has training
Educate that the period is short, process for producing is simple, the high production efficiency of product and the features such as stablize.
4, by applying the present invention, formed dendrobium candidum phellinus igniarius mycelium it is widely used, can be used as be prepared into drug,
The raw material of health care product, Speciality Foods additive.Also can be sub-packed in capsule becomes a kind of characteristic and health care food drug.Not only have non-
Often good market prospects, and the economic benefit generated also can highly significant.
Specific embodiment
Method of the invention is further illustrated below with reference to embodiment.
Phellinus igniarius mycelium is prepared by culture medium of dendrobium candidum, be in technical solution: the mycelium is to use dendrobium candidum
Stem section is primary raw material to do culture medium, Phellinus strain will be accessed after medium sterilization, then cultivates to mycelia and cover with culture medium,
Then the culture medium for covering with mycelia is dried, finally crushes to obtain dendrobium candidum phellinus igniarius mycelium.
Specific embodiment is as follows:
1. dendrobium candidum acquisition and processing: acquisition it is disease-free, without insect pest, non agricultural chemical residuum and fresh dendrobium candidum be original
Material, then peels the leaf of dendrobium candidum off, then dendrobium candidum is cut into 1-2 centimetres of segment, finally dendrobium candidum segment
It is dried to that material moisture is spare lower than 8%.
2. preparing culture medium: culture medium is made of 90% dendrobium candidum segment and 10% corn flour, first by iron sheet stone
Dry measure used in former times segment is soaked among 5% glucose water, is pulled out and is drained away the water after persistently impregnating 8-9 hour, corn flour also uses 5%
It is 55-60% that glucose water, which is adjusted to moisture content, and then dendrobium candidum and corn flour are mixed again and stir evenly to obtain mixing
Material is sub-packed in mixed material in culture vessel, and charge weight is that thickness of the mixed material in culture vessel is 2-3cm, most
Production culture medium is obtained after conventionally sterilizing afterwards.
3. inoculation: Phellinus strain being accessed production culture medium in an aseptic environment, when accessing solid Phellinus strain, to be connect
Enter the solid spawn of 7-8 point, when accessing Phellinus liquid spawn, to access the 10-12% liquid spawn of culture medium weight.
4. cultivation: the culture vessel that will be connected kind moves on to culturing room and carries out being protected from light culture, is 20~23 DEG C, relatively in temperature
Culture to mycelia covers with culture medium in the environment of humidity is 60~65%.
5. drying: shining the culture medium for covering with mycelia to material moisture lower than 8% in the environment of temperature is lower than 50 degree.
6. finished product: the medium powder dried, which is broken to, which can cross 120-200 mesh, obtains finished product dendrobium candidum phellinus igniarius mycelium.
Claims (1)
1. the method for preparing phellinus igniarius mycelium as culture medium using dendrobium candidum, it is characterised in that: the mycelium is to use dendrobium candidum
Stem section is primary raw material to do culture medium, Phellinus strain will be accessed after medium sterilization, then cultivates to mycelia and cover with culture medium,
Then the culture medium for covering with mycelia is dried, finally crushes to obtain dendrobium candidum phellinus igniarius mycelium；
Specific preparation step are as follows:
(1) dendrobium candidum acquisition and processing: acquisition it is disease-free, without insect pest, non agricultural chemical residuum and fresh dendrobium candidum be raw material,
Then the leaf of dendrobium candidum is peeled off, then dendrobium candidum is cut into 1-2 centimetres of segment, finally dendrobium candidum segment is dried
It is spare lower than 8% to material moisture；
(2) prepare culture medium: culture medium is made of 90% dendrobium candidum segment and 10% corn flour, first by dendrobium candidum
Segment is soaked among 5% glucose water, is pulled out and is drained away the water after persistently impregnating 8-9 hour, corn flour also uses 5%
It is 55-60% that glucose water, which is adjusted to moisture content, and then dendrobium candidum and corn flour are mixed again and stir evenly mixed
Material is closed, mixed material is sub-packed in culture vessel, charge weight is that thickness of the mixed material in culture vessel is 2-3cm,
Production culture medium is obtained after finally conventionally sterilizing；
(3) it is inoculated with: Phellinus strain being accessed production culture medium in an aseptic environment, when accessing solid Phellinus strain, to access
The solid spawn of 7-8 point will access the 10-12% liquid spawn of culture medium weight when accessing Phellinus liquid spawn；
(4) cultivation: the culture vessel that will be connected kind moves on to culturing room and carries out being protected from light culture, temperature be 20~23 DEG C, it is relatively wet
Culture to mycelia covers with culture medium in the environment of degree is 60~65%；
(5) it dries: shining the culture medium for covering with mycelia to material moisture lower than 8% in the environment of temperature is lower than 50 degree；
(6) finished product: the medium powder dried, which is broken to, which can cross 120-200 mesh, obtains finished product dendrobium candidum phellinus igniarius mycelium.
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| CN104285669A (en) * | 2014-09-18 | 2015-01-21 | 淳安千岛湖桑都食用菌专业合作社 | Phellinus igniarius bacterial strain and application of phellinus igniarius bacterial strain |
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2016
- 2016-06-28 CN CN201610482734.7A patent/CN105900696B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060116463A (en) * | 2005-05-10 | 2006-11-15 | 배만종 | The hyphae of mushroom cultured in ginseng dregs, and the culturing method |
| CN101743854A (en) * | 2009-12-09 | 2010-06-23 | 浙江森宇实业有限公司 | Method for preparing morchella mycelium by using dendrobium fiber as substrate |
| CN103299824A (en) * | 2012-03-16 | 2013-09-18 | 何寒 | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn |
| CN103583236A (en) * | 2013-11-16 | 2014-02-19 | 覃秀花 | Method for preparing fleckedflesh polypore mycelium |
| CN105359821A (en) * | 2014-09-02 | 2016-03-02 | 许超群 | Manufacturing phellinus igniarius hypha block by sprouted niblet |
| CN104285669A (en) * | 2014-09-18 | 2015-01-21 | 淳安千岛湖桑都食用菌专业合作社 | Phellinus igniarius bacterial strain and application of phellinus igniarius bacterial strain |
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