CN102424625B - Edible and medical fungi blackening agent, its preparation method and application - Google Patents
Edible and medical fungi blackening agent, its preparation method and application Download PDFInfo
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Abstract
The invention discloses an edible and medical fungi blackening agent and specifically relates to the technical field of biological fermentation. Natural components with a blackening effect are fermented by edible and medical fungi so as to obtain zymogeneous bacteria. The bacteria contain higher nutrients than natural raw materials, and contain effective ingredients as well as amino acid, vitamin, trace elements and natural plant melanin which are necessary for the hair growth. The edible and medical fungi blackening agent has a more ideal effect for hair dyeing and blackening than general plant hair dyes, has a wide source of raw materials, requires low price, and can bring benefit for common people. The product provided by the invention contains no chemical hair dye component, is safe and reliable to use, has no toxic and side effect, and is a product which has a health-care function and also can be used to fundamentally treat leukotrichia and alopecia.
Description
Technical field
The invention belongs to technical field of biological fermentation, be specifically related to by edible medicinal fungus fermentative production blackening agent and production technology thereof.
Background technology
Hair is the important component part of human body, is one of important symbol of appearance fitness.Along with the development of social civilization, the improving constantly of social life level, people pursue the perfection of self day by day with healthy, and having a healthy and beautiful healthy hair is the hope that many people dream of.Yet along with current people's rhythm of life is accelerated, operating pressure and stress increase, the factors such as wretched insufficiency of having a rest, sleep cause hair desired nutritional unbalance, cause young and middle-aged white hair crowd more and more.White hair has become the important factor that affects people's image and psychosomatic health, approximately there are 1.5～1.8 hundred million hoary and white hair patients in China, approximately there are 5～600,000,000 hoary and white hair patients in the whole world, the cause of disease more complicated of white hair, according to the study, people's hair is likely that by black bleaching melanic melanocyte occurs extremely to cause owing to supplying to hair.In addition, obstacle, endocrine disturbance and the melanocyte of heredity, direct stimulation are lost disease and also can be caused white hair, and this is also chief reason very.
The traditional Chinese medical science is thought " hair is the sign of blood condition ", and " the main bone of kidney, its sliding sending out " advocates that the food that white hair patient eats blood rearing interpolating kidney more sends out with Wu Farun.If can coordinate the method results for the treatment of such as Chinese medicine external, food meals therapy, massage better, above-mentioned therapy has no side effect, is applicable to the advantages such as any ages use, but in this method short period of time, effect is not remarkable, slow, the shortcoming such as curative ratio is low, strict implement is difficult to adhere to takes effect, do not meet modern people's economic and social life rhythm, make majority's gave up halfway, therefore more difficult applying.
Hair dyeing is as one of mode of improving looks, and consumer groups constantly expand in recent years.Hair dyeing, the blackening agent produced both at home and abroad at present, mainly contain chemistry and plant two series products.
Hair dye used is through chemosynthesis mostly at present, although chemical hair dye color and luster nature, easy to operate, dyeing firmly, but chemical hair dye is the hair scale sheet of opening by force hair, dying rear hair scale sheet cannot be completely closed, cause that hair quality is coarse, withered, bifurcated, easily broken, even stop up hair follicle hole, cause alopecia, simultaneously to take " Ursol D " class chemical substance be main raw material due to most in chemical hair dye, has certain toxicity and carinogenicity, strong sensitization, can bring out various diseases, have certain healthy hidden danger.
Human use's natural phant is had hair dyed and is had thousands of years history.Ancient egypt people, Roman, Chinese and Indian, used the hair dyeing of plant (juice) pigment very early.It is low that plant hair dyeing has toxicity, uses safe feature, becomes in recent years various countries investigator's study hotspot.Natural botanical hair dye raw material is mainly the organic substance extracting from plant, although pungency is relatively little, few to hair damage, hair dyeing effect has certain limitation, and fast colours degree is poor, easily decolouring, the few and price of raw material.
In a word, on current market, publicity has hair washing, the profit that crow sends out function and sends out, nourishes product etc., no matter is external application or product for oral administration, and the product that really natural, effective, safety has no side effect is few.Especially in recent years, there are quality problems in the hair dye of each famous brand, blackening agent again and again, makes people have many doubts when using hair dyeing, blackening agent.People are eager expects have natural element to make, the appearance of blackening and beautifying hair product safely and effectively.
Summary of the invention
In order to address the above problem, be to overcome the deficiency of chemical hair dye and general botanical hair dye order of the present invention, give full play to the function of bacterium for food (medicine), produce more safe and reliable, healthy novel blackening agent.The prepared hair dyeing of the present invention, blackening agent are nutritious, contain 30～40% protein, 17 seed amino acids, 15～20% active polysaccharide, 0.1～1.76% triterpene compound, and contain the mineral elements such as abundant VITAMIN and calcium, magnesium, zinc, often use can significantly improve immune function of human body, have and protect kidney benefit lung, liver protecting, hypoglycemic, reducing blood-fat, improve gastrointestinal function, endocrine regulation, with this product, smearing scalp parallel port and take 30 days and can naturally grow black, is a kind ofly to have nourishing function can fundamentally administer the product of white hair again.Above-mentioned purpose of the present invention realizes by following technique means:
The blackening agent that the present invention adopts food (medicine) to produce with bacterium fermentation technique, in culture medium raw material employing classical TCM, having plant, animal, the medicinal fungi scientific matching that crow sends out, profit is sent out effect forms, and carry out liquid or solid fermentation with fungal bacterial strain, mycelium and fermented liquid that acquisition is contained to various active composition and nutrition are made blackening agent, wherein:
Described culture medium dry powder raw material is the mixture of black rice and following one or more materials: black soya bean, Semen Sesami Nigrum, Formica fusca, glossy ganoderma, ginseng, Tuber Fleeceflower Root, Radix Angelicae Sinensis, mulberries, Ginkgo Leaf.
Described fungi fermentation bacterial strain is the mixture of following 1～3 kind of fungi: Cordyceps militaris (L.) Link. [Cordyceps militaris (Lex.Fr) Link], Cordyceps sinensis (Sphaeria sinensis), red ganoderma (Ganoderma lucidum), Ganoderma sinense Zhao, Xu et Zhang (Ganoderma neo-japonicum Imazeki), Black Ganoderma (Ganoderma), Phaeopoms obliquus (Inonqqus obliquus), Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. [Laetiporus sulphureus (Fr.) Murrill], black fungus (Auricularia or Wood ear), Coprinus comatus bacterium [Copyinds comatus (MUII.Fr) Gray].
The present invention adopts a kind of novel food (medicine) to use bacterium culture medium dry powder, comprise black rice and auxiliary material, wherein the quality percentage composition of black rice is 1～90%, and one or more in following component of wherein said auxiliary material form by weight: black soya bean 1～50, Semen Sesami Nigrum 1～40, Formica fusca 1～10, glossy ganoderma 1～20, ginseng 0.1～8, Tuber Fleeceflower Root 1～30, Radix Angelicae Sinensis 1～20, mulberries 1～20, Ginkgo Leaf 1～20;
The invention still further relates to a kind of food (medicine) and use bacterium blackening agent, it is characterized in that using above-mentioned food (medicine) to use bacterium culture medium dry powder, method through fungi fermentation makes, it is characterized in that, fermented bacterium is 1～3 kind of following fungi, and total inoculum size of bacterial classification is 4～10% of culture medium dry powder total mass: Cordyceps militaris (L.) Link., Cordyceps sinensis, red ganoderma, Ganoderma sinense Zhao, Xu et Zhang, Black Ganoderma, Phaeopoms obliquus, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., black fungus, Coprinus comatus bacterium.
Above-mentioned blackening agent, when used medium dry powder raw material and fermented bacterium are used by following condition, effect is better:
Culture medium dry powder raw material mixes as follows by quality percentage composition:
Black rice 10～80
Auxiliary material surplus
Wherein, auxiliary material consists of by weight one or more following materials: black soya bean 10～45, Semen Sesami Nigrum 5～30, Formica fusca 2～8, glossy ganoderma 2～20, ginseng 1～7, Tuber Fleeceflower Root 1～20, Radix Angelicae Sinensis 3～15, mulberries 2～15, Ginkgo Leaf 2～18;
Its fermented bacterium is 1～2 kind in Cordyceps militaris (L.) Link., Cordyceps sinensis, red ganoderma, Ganoderma sinense Zhao, Xu et Zhang, Black Ganoderma, Phaeopoms obliquus, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer., black fungus, Coprinus comatus bacterium, and inoculum size is 4～10% of culture medium dry powder total mass.
Above-mentioned blackening agent, when used medium dry powder raw material and fermented bacterium are used by following condition, best results: culture medium dry powder raw material mixes as follows by quality percentage composition:
Black rice 15～65
Auxiliary material surplus
Wherein, auxiliary material mixes as follows by one or more following materials by weight: black soya bean 15～40, Semen Sesami Nigrum 6～28, Formica fusca 3～7, Ganoderma spore powder or Ganoderma powder 5～15, ginseng 2～6, Tuber Fleeceflower Root 8～18, Radix Angelicae Sinensis 5～10, mulberries 4～12, Ginkgo Leaf 4～15;
Its fermented bacterium is Cordyceps militaris (L.) Link. or Black Ganoderma, and inoculum size is 4～10% of culture medium dry powder total mass.
The preparation method of above-mentioned blackening agent, its step of preparation process is as follows:
(a) various culture medium raw materials are mixed in proportion to rear pulverizing;
(b) to the water that adds 1～10 times of quality in the mixture of step a gained, after sterilizing, make substratum;
(c), in the substratum of step b gained, access in proportion fermented bacterium;
(d) under 20～27 ℃ of conditions, cultivate 10～60 days, obtain tunning;
(e) tunning steps d being obtained is centrifugal, and collecting precipitation thing and centrifugate are made blackening agent product respectively.
In above-mentioned preparation method, the pulverized size described in step a will be selected according to different fermentation process, when adopting liquid fermentation process, by raw material pulverizing to 40～100 order, when adopting solid fermentation method, by raw material pulverizing to 1～2mm.
In above-mentioned preparation method, the water pH described in step b is 7～8 o'clock, and the blackening agent effect making is better.
In above-mentioned preparation method, the centrifugal product described in step e is processed better through following manner, and throw out is made oral blackening agent product, and described centrifugate is made the blackening agent product of external application.
In above-mentioned preparation method, the formulation of the blackening agent product described in step e is: electuary, pill, tablet, capsule, paste, decoction, syrup, aerosol.
Above-mentioned blackening agent can be applied in hair dyeing widely, crow sends out, treats the fields such as white hair.
Product of the present invention can not only be had hair dyed, and can bear again black, fundamentally remove white hair, reach black object all over the face, the effect that having takes stopgap measures effects a permanent cure, its mechanism is plant, animal, the fungi raw material of sending out for the manufacture of hair dyeing crow, after food (medicine) ferments with bacterium, can significantly improve nutrition and effective constituent in raw material, have the effect of 1+1 > 2.As added 5% Ganoderma spore powder in raw material, there is important anti-tumor activity, and three content that paste compounds that improve immunizing power are 0.01% and can be increased to 0.67% after fungi fermentation, improve 67 times.Have significantly antitumor, antivirally, improve body immunity, repair corrupted cell, regulate blood sugar blood matter, cholesterol, the function such as hypotensive, and the skin of can also moistening, the happy pool of appearance, hairdressing crow sends out, and eliminates senile plaque.Modern medicine study shows, food (medicine) is used relevant with its function with the outbreak of bacterium hairdressing crow, it can see through skin surface and promote skin blood microcirculation, deep layer is moistened the hair papilla of skin of head, improve ball top portion hair matricyte nutrition supply level, effectively remove seborrheic plug, dirt and other the bad metabolite stopping up in hair follicle, activate hair papilla cell, make it recover the melanic function of secretion.
Beneficial effect:
Before this patent product, the hair dyeing, the hair blackening product that there are no food (medicine), with bacterium fermentation method, are prepared from.This product, containing any chemistry hair dyeing composition, is not used safety, without appointing, closes toxic side effect, can not only be for smearing, and oral also safety.Smear and can grow natural crow in oral 30 days and send out.
Below in conjunction with several embodiment, the present invention is described further.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Test method described in following embodiment, if no special instructions, is ordinary method; Described raw material all can obtain from commercial channels.
Black rice, black soya bean, Semen Sesami Nigrum are purchased from the market of farm produce;
Formica fusca is purchased from Hengxian County pure Formica fusca powder factory;
Ganoderma spore powder, Ganoderma powder are purchased from glossy ganoderma base, Guan County, Shandong;
Tuber Fleeceflower Root, ginseng, Radix Angelicae Sinensis, mulberries, Ginkgo Leaf are purchased from shop of Chinese medicines;
Cordyceps militaris spawn, Black Ganoderma bacterial classification are purchased from precious gill fungus fungus development centre, LiaoNing, China province Dalian;
Embodiment 1
(1) preparation of edible medicinal fungus blackening agent
(a) get black rice 1750g, black soya bean 700g, Semen Sesami Nigrum 525g, Tuber Fleeceflower Root 350g, Radix Angelicae Sinensis 175g that fresh nothing goes mouldy, be crushed to 100 orders after mixing;
(b) to the tap water that adds 35000ml pH7.5 in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum to step b gained is cooled to after 30 ℃, accesses Black Ganoderma liquid spawn under aseptic condition, thalline content 40wt%, the 350g that weighs (thalline weight in wet base is weighed as 140g);
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7～0.8kg/cm
2, air flow 1: 1v/v/ divides, stops fermentation in 10 days afterwards, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40～50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material.
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2～1.35, makes blackening agent paste raw materials.
(2) finished product detection
With Kjeldahl determination, recording protein content in product is 30%; High performance liquid chromatography (High Performance Liquid Chromatography, HPLC) detects and contains 17 seed amino acids; Phenolsulfuric acid method records the active polysaccharide that contains 15%, and high performance liquid chromatography records triterpene compound 0.1%.
(3) effect detection
People's hair dyeing test: with making blackening agent paste raw materials, smear scalp every day once, massage 10 minutes, and oral 2g/ time, every day 3 times, after 30 days, the blackening of white hair root.Hair dyeing person's basic condition table is as shown in table 1, wherein has in 80 different crowds of accepting to detect efficient having reached more than 95%.
Table 1 hair dyeing test effect
Embodiment 2
(1) preparation of edible medicinal fungus blackening agent
(a) get black rice 700g, black soya bean 1050g, Semen Sesami Nigrum 700g, Ganoderma spore powder 350g, Tuber Fleeceflower Root 350g, the Ginkgo Leaf 350g that fresh nothing goes mouldy and be crushed to 100 orders after mixing;
(b) to the pH7.5 tap water that adds 35000ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum to step b gained is cooled to after 30 ℃, accesses Black Ganoderma liquid spawn under aseptic condition, thalline content 40wt%, the 350g that weighs (thalline weight in wet base is weighed as 140g);
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7～0.8kg/cm
2, air flow 1: 1v/v/ divides, stops fermentation in 10 days afterwards, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40～50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material.
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2～1.35, makes blackening agent paste raw materials.
(2) finished product detection
With Kjeldahl determination, recording protein content in product is 35%; High performance liquid chromatography detects and contains 17 seed amino acids; Phenolsulfuric acid method records the active polysaccharide that contains 17%, and high performance liquid chromatography records triterpene compound 1.76%.
(3) effect detection
People's hair dyeing test: with making blackening agent paste raw materials, smear scalp every day once, massage 10 minutes, and oral blackening agent raw material 1.5g/ time, every day 3 times, after 30 days, the blackening of white hair root, grows black fine hair.Hair dyeing person's basic condition table is as shown in table 2, wherein has in 120 different crowds of accepting to detect efficient having reached more than 97%.
Table 2 hair dyeing test effect
Embodiment 3
(1) preparation of edible medicinal fungus blackening agent
(a) preparation substratum: get black rice 525g, black soya bean 700g, Semen Sesami Nigrum 525g, Formica fusca 175g Ganoderma spore powder 350g, Tuber Fleeceflower Root 525g, Radix Angelicae Sinensis 175g, mulberries 175g, the Ginkgo Leaf 175g ginseng 175g that fresh nothing goes mouldy and be crushed to 100 orders after mixing.
(b) to the pH7.5 tap water that adds 35000ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum to step b gained is cooled to after 30 ℃, accesses Cordyceps militaris bacterial classification under aseptic condition, thalline content 40wt%, the 350g that weighs (thalline weight in wet base is weighed as 140g);
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7～0.8kg/cm
2, air flow 1: 1v/v/ divides, stops fermentation in 10 days afterwards, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40～50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material.
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2～1.35, makes blackening agent paste raw materials.
(2) finished product detection
With Kjeldahl determination, recording protein content in product is 37%; High performance liquid chromatography detects and contains 17 seed amino acids; Phenolsulfuric acid method records the active polysaccharide that contains 20%, and high performance liquid chromatography records triterpene compound 1%.
(3) effect detection
People's hair dyeing test: with making blackening agent paste raw materials, smear scalp every day once, massage 10 minutes, and oral blackening agent raw material 1.5g/ time, every day 3 times, after 30 days, the blackening of white hair root, grows black fine hair.Hair dyeing person's basic condition table is as shown in table 3, wherein has in 100 different crowds of accepting to detect efficient having reached more than 92%.
Table 3 hair dyeing test effect
Embodiment 4
(1) preparation of edible medicinal fungus blackening agent
(a) preparation substratum: get black rice 1750g, black soya bean 700g, Semen Sesami Nigrum 525g, Tuber Fleeceflower Root 350g, Radix Angelicae Sinensis 175g that fresh nothing goes mouldy, be crushed to 1-2mm after mixing;
(b) to the pH7.5 tap water that adds 3500ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum to step b gained is cooled to after 30 ℃, accesses the Cordyceps militaris spawn weight in wet base 350g that weighs under aseptic condition;
(d) culture condition: cultivate for 27 ± 1 ℃ and stop afterwards fermentation for 45 days;
(e) fermented liquid steps d being obtained, in 40～50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent raw material;
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2～1.35, makes blackening agent paste raw materials.
(2) finished product detection
With Kjeldahl determination, recording protein content in product is 32%; High performance liquid chromatography detects and contains 17 seed amino acids; Phenolsulfuric acid method records the active polysaccharide that contains 18%, and high performance liquid chromatography records triterpene compound 0.2%.
(3) effect detection
People's hair dyeing test: with making paste blackening agent, smear scalp every day once, massage 10 minutes, and oral blackening agent raw material 1.5g/ time, every day 3 times, after 30 days, the blackening of white hair root, grows black fine hair.Hair dyeing person's basic condition table is as shown in table 4, wherein has in 100 different crowds of accepting to detect efficient having reached more than 93%.
Table 4 hair dyeing test effect
As seen from the above-described embodiment: the bacterium blackening agent of having hair dyed for the food the present invention relates to (medicine), by having natural component that crow sends out effect through edible, medicinal fungi fermentation, obtains Zymocyte.In this mycoplasma, contain the required amino acid of the nutrition higher than natural matter, effective ingredient and natural on-off cycles of hair growth, natural phant melanochrome etc., not containing appointing combination hair dye composition.With its hair dyeing crow, send out more desirable than general botanical hair dye effect, and the raw materials used easy purchase of the present invention, source is wide, price is low, safely, have no side effect, be a kind of nourishing function that has, can administer again the product of hoariness and alopecia.
Claims (2)
1. an edible medicinal fungus blackening agent, is characterized in that, the preparation method of use is as follows:
(a) get black rice 1750g, black soya bean 700g, Semen Sesami Nigrum 525g, Tuber Fleeceflower Root 350g, the Radix Angelicae Sinensis 175g that fresh nothing goes mouldy and be crushed to 100 orders after mixing;
(b) to the tap water that adds 35000ml pH7.5 in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum of step b gained is cooled to after 30 ℃, under aseptic condition, accesses Black Ganoderma liquid spawn, thalline content 40wt%, the 350g that weighs, thalline weight in wet base is weighed as 140g;
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7 ~ 0.8kg/ cm
2, air flow 1:1v/v/ divides, and within 10 days, stops afterwards fermentation, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40 ~ 50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material;
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2 ~ 1.35, makes blackening agent paste raw materials.
2. an edible medicinal fungus blackening agent, is characterized in that, the preparation method of use is as follows:
(a) get black rice 700g, black soya bean 1050g, Semen Sesami Nigrum 700g, Ganoderma spore powder 350g, Tuber Fleeceflower Root 350g, the Ginkgo Leaf 350g that fresh nothing goes mouldy and be crushed to 100 orders after mixing;
(b) to the pH7.5 tap water that adds 35000ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2 kg/cm
2, make substratum after maintaining 40min;
(c) substratum of step b gained is cooled to after 30 ℃, under aseptic condition, accesses Black Ganoderma liquid spawn, thalline content 40wt%, the 350g that weighs, thalline weight in wet base is weighed as 140g;
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7 ~ 0.8kg/cm
2, air flow 1:1v/v/ divides, and within 10 days, stops afterwards fermentation, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40 ~ 50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material;
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2 ~ 1.35, makes blackening agent paste raw materials.
3. an edible medicinal fungus blackening agent, is characterized in that, the preparation method of use is as follows:
(a) preparation substratum: get black rice 525g, black soya bean 700g, Semen Sesami Nigrum 525g, Formica fusca 175g, Ganoderma spore powder 350g, Tuber Fleeceflower Root 525g, Radix Angelicae Sinensis 175g, mulberries 175g, Ginkgo Leaf 175g, the ginseng 175g that fresh nothing goes mouldy and be crushed to 100 orders after mixing;
(b) to the pH7.5 tap water that adds 35000ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2 kg/cm
2, make substratum after maintaining 40min;
(c) substratum of step b gained is cooled to after 30 ℃, under aseptic condition, accesses Cordyceps militaris bacterial classification, thalline content 40wt%, the 350g that weighs, thalline weight in wet base is weighed as 140g;
(d) culture condition: 27 ± 1 ℃, tank pressure 0.7 ~ 0.8kg/ cm
2, air flow 1:1v/v/ divides, and within 10 days, stops afterwards fermentation, then through 3000 revs/min, after centrifugal 10 minutes, obtains fermentation mycelium and fermented liquid;
(e) fermentation mycelium steps d being obtained, in 40 ~ 50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent oral preparation raw material;
(f)the fermented liquid that steps d is obtained, with haplo-effect concentrator vacuum concentration to 1.2 ~ 1.35, makes blackening agent paste raw materials.
4. an edible medicinal fungus blackening agent, is characterized in that, the preparation method of use is as follows:
(a) preparation substratum: get black rice 1750g, black soya bean 700g, Semen Sesami Nigrum 525g, Tuber Fleeceflower Root 350g, the Radix Angelicae Sinensis 175g that fresh nothing goes mouldy and be crushed to 1-2mm after mixing;
(b) to the pH7.5 tap water that adds 3500ml in the mixture of step a gained, high pressure steam sterilization under 127 ℃ of conditions, pressure 1.2kg/cm
2, make substratum after maintaining 40min;
(c) substratum of step b gained is cooled to after 30 ℃, under aseptic condition, accesses the Cordyceps militaris spawn weight in wet base 350g that weighs;
(d) culture condition: cultivate for 27 ± 1 ℃ and stop afterwards fermentation for 45 days;
(e) fermented liquid steps d being obtained, in 40 ~ 50 ℃ of oven dry 24h, is crushed to below 100nm through nano traditional Chinese medicine cell wall breaking pulverizer, makes blackening agent raw material;
(f) fermented liquid steps d being obtained, with haplo-effect concentrator vacuum concentration to 1.2 ~ 1.35, makes blackening agent paste raw materials.
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| CN102766658B (en) * | 2012-07-30 | 2014-05-21 | 南京农业大学 | Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin |
| CN104303830A (en) * | 2014-09-28 | 2015-01-28 | 铜陵市香江食用菌种植有限责任公司 | Planting method of Pleurotus eryngii |
| CN104434695A (en) * | 2014-12-16 | 2015-03-25 | 常熟市联创化学有限公司 | Novel hair dye |
| CN104689203A (en) * | 2015-03-21 | 2015-06-10 | 石汝花 | Capsule for treating alopecia and preparation method thereof |
| CN107041903B (en) * | 2016-02-05 | 2021-03-09 | 中国中医科学院中药研究所 | Integrated new method for processing and concocting radix Polygoni Multiflori Preparata in producing area |
| CN107156801A (en) * | 2016-03-08 | 2017-09-15 | 及长城 | A kind of active mycelium group compound with hair tonic and preparation method thereof |
| CN107258971A (en) * | 2017-05-24 | 2017-10-20 | 天津瑞特医疗保健用品开发有限公司 | With alopecia-stopping, black hair, hair tonic, the compound black tea sugar of face-nursing function and its preparation method and application |
| CN107184517B (en) * | 2017-06-02 | 2020-04-28 | 天津郁美净集团有限公司 | Hair care composition and preparation method thereof |
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| CN1069108C (en) * | 1998-08-26 | 2001-08-01 | 姜勇 | Black wine |
| CN101019816A (en) * | 2006-02-16 | 2007-08-22 | 茹国军 | Prepn process of hair blackening shampoo |
| CN101292693A (en) * | 2007-04-26 | 2008-10-29 | 李树唐 | Health tea for blackening and generating hair |
| CN101181216A (en) * | 2007-11-29 | 2008-05-21 | 王晓红 | Hair-blackening Chinese medicine shampoo and manufacturing method thereof |
| CN101485831B (en) * | 2009-03-02 | 2011-06-15 | 王立国 | Chinese medicinal composition for blackening and generating hair and preparation method thereof |
| CN102091278A (en) * | 2010-09-08 | 2011-06-15 | 成进学 | Selenium- and germanium-enriched lucid ganoderma mycelium snake nano metallothionein capsule |
| CN101940717B (en) * | 2010-09-29 | 2013-03-27 | 湖南省永佳阿胶制药有限公司 | Oral administration prescription for hair blacking and baldness prevention and preparation method and using method thereof |
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