CN103621315B - Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution - Google Patents
Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution Download PDFInfo
- Publication number
- CN103621315B CN103621315B CN201310671995.XA CN201310671995A CN103621315B CN 103621315 B CN103621315 B CN 103621315B CN 201310671995 A CN201310671995 A CN 201310671995A CN 103621315 B CN103621315 B CN 103621315B
- Authority
- CN
- China
- Prior art keywords
- herbal medicine
- chinese herbal
- cordyceps militaris
- bacterium liquid
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for cultivating cordyceps militaris by using a Chinese herbal medicine nutrient solution, and belongs to the field of fungus cultivation. The method comprises the following steps of using hot water extraction to concentrate a Chinese herbal medicine prescription to obtain a Chinese herbal medicine prescription extract, and adding bran, rice flour, corn flour, soy flour, brown sugar and the like in the Chinese herbal medicine prescription extract to obtain a Chinese herbal medicine nutrient solution culture medium suitable for the growth of cordyceps militaris. The culture medium is sterilized by autoclaving and is cooled, and then the cordyceps militaris bacteria are introduced into the culture medium. After the bacteria are stirred by a magnetic stirrer and are cultivated, a bacterium solution is dispensed in cultivation bottles, and the cordyceps militaris is cultivated in a bacterium solution shallow layer. The method has the advantages of short period, simplicity in operation, low investment and low cost, and the product has higher edible, medicinal and economic values.
Description
Technical field
The invention belongs to mushroom cultivation field, be specifically related to a kind of method adopting Chinese medicinal herb nutrient liquid to cultivate Cordceps militaris.
Background technology
China is the cradle of Chinese herbal medicine, and ancient times, the sage of the past was to the further investigation of Chinese herbal medicine and traditional Chinese medicine and pharmacy, research and summary, made Chinese herbal medicine obtain approval the most widely and application.Chinese herbal medicine is the unique pharmaceutical that prevention with Chinese medicine disease therapy uses, and the function composition of Chinese herbal medicine is transferred on mushroom Cordceps militaris, and people have eaten Cordceps militaris and nutritious, have medicinal function again.The present invention adopts Chinese herbal medicine prescription (Radix Astragali, Radix Angelicae Sinensis, matrimony vine, barrenwort, cynomorium songaricum, the glossy ganoderma etc.) extract of the foster kidney that fills blood to add appropriate nutriment soybean meal, corn flour etc. are mixed with Chinese medicinal herb nutrient liquid culture medium, adopt bacterium liquid shallow-layer to cultivate Cordceps militaris.It is short, easy and simple to handle that the Cordceps militaris that the method is produced has the cycle, and small investment, cost is low, and product has very high edible, medicinal and economic worth.
Summary of the invention
The object of the present invention is to provide a kind of method adopting Chinese medicinal herb nutrient liquid to cultivate Cordceps militaris, the Cordceps militaris that the method is produced, the cycle is short, easy and simple to handle, small investment, and cost is low, and product has higher edible, medicinal and economic worth.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method adopting Chinese medicinal herb nutrient liquid to cultivate Cordceps militaris is in extracts of Chinese herbal medicine, add the auxiliary materials such as wheat bran, rice meal, corn flour, analysis for soybean powder and brown sugar, be mixed with Chinese medicinal herb nutrient liquid culture medium, magnetic stirrer stir culture bacterium liquid is adopted after inoculation, then be sub-packed in culture bottle, bacterium liquid shallow-layer cultivates Cordceps militaris.
Comprise the following steps:
(1) preparation of Chinese medicinal herb nutrient liquid culture medium and bacterium liquid are cultivated;
(2) bacterium liquid shallow-layer cultivates Cordceps militaris.
The preparation of described Chinese medicinal herb nutrient liquid culture medium and bacterium liquid are cultivated and are comprised the following steps:
(1) Radix Astragali 200g, Radix Angelicae Sinensis 150g, matrimony vine 150g, barrenwort 100g, cynomorium songaricum 100g, glossy ganoderma 100g, adding distil water 16000mL, after boiling, put in drying baker in pot, and 80 DEG C of lixiviate 16h, are concentrated into 10000mL, obtain extracts of Chinese herbal medicine, for subsequent use;
(2) in extracts of Chinese herbal medicine 2000mL, add glucose 20g, wheat bran 20g, rice meal 20 g, corn flour 20g, analysis for soybean powder 15g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, peptone 5g and 21 gold medals tie up him one, mix;
(3) medium is prepared according to a conventional method, bottling; Autoclaving, (pressure is 0.7kg/cm in 115 DEG C of pressurizes
2) 60-90min; Inoculation, every bottle of inoculum concentration is 15%-20%; Adopt magnetic stirrer, 22 DEG C of light culture 5-7 days.
Described bacterium liquid shallow-layer is cultivated Cordceps militaris and is comprised the following steps: bacterium liquid is sub-packed in culture bottle, and culture bottle will be sterilized for subsequent use in advance, every bottled bacterium liquid height 1cm, 18 DEG C of light culture 7-8 days; Then h light cultivation every day 12 is proceeded to, intensity of illumination 200 lx, temperature 10 DEG C-20 DEG C, every temperature difference per day 10 DEG C; When scarlet caterpiller fungus mycelium becomes orange colour from white, and when starting to occur former base, regulate temperature and light photograph, temperature 18 DEG C-20 DEG C, whole day illumination cultivation, intensity of illumination 1000-1500 lx, humidity 80%-90%, air is fresh, gathers time ripe to stroma.
Remarkable advantage of the present invention is:
(1) Chinese medicinal herb nutrient liquid shallow-layer is cultivated Cordceps militaris and can be nurtured Cordceps militaris at short notice.
(2) there is the Cordceps militaris of Chinese herbal medicine composition, improve edible, medicinal and economic worth is high.
(3) cultivating method is simple, and the cycle is short, and output is high, and quality is good, and price is high.
Embodiment
embodiment 1
(1) Chinese medicinal herb nutrient liquid culture medium is prepared:
Extract liquid, Radix Astragali 200g, Radix Angelicae Sinensis 150g, matrimony vine 150g, barrenwort 100g, cynomorium songaricum 100g, glossy ganoderma 100g adding distil water 16000 milliliters, put after boiling in pot, put in drying baker, and 80 DEG C of lixiviate 16h, are concentrated into 10000ml, for subsequent use.
Culture medium prescription: liquid 2000ml, glucose 20g, wheat bran 20g, rice meal 20g, corn flour 20g, analysis for soybean powder 15g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, peptone 5g, 21 gold medals tie up him one.
Prepare medium according to a conventional method, be sub-packed in 1000 ml triangular flasks, often bottled 800 ml nutrient solutions, put into a magnet rotor, seal with silica gel plug; Adopt autoclaving, 115 DEG C of dwell times are 90min.
(2) inoculate, cultivate:
1) inoculate, after medium cooling, every bottle graft enters Cordceps militaris bacterial classification 150g solid kind or 150ml liquid strain.
2) cultivate, be placed on stir culture on magnetic stirrer after inoculation, light culture, cultivate 5 ~ 7 days for 22 DEG C.
3) bacterium liquid shallow-layer cultivates Cordceps militaris: bacterium liquid is sub-packed in culture bottle (500ml tissue culture bottle), culture bottle will be sterilized for subsequent use in advance, every bottled bacterium liquid 25ml,
4) culture bottle Mycelium culture: 20 DEG C of light culture, after 7 ~ 8 days, proceed to illumination cultivation, every day 12 h light, intensity of illumination 200 lx, temperature 10 DEG C ~ 20 DEG C, every temperature difference per day 10 DEG C.
5) stroma growth management, when scarlet caterpiller fungus mycelium becomes orange colour from white, and when starting to occur former base, regulates temperature and light to shine, temperature 18 ~ 20 DEG C, whole day illumination cultivation, intensity of illumination 1000 ~ 1500 lx, humidity 80% ~ 90%, air is fresh, gathers time ripe to stroma.
Growth cycle 40 ~ 45 days, often liter of Chinese medicinal herb nutrient liquid produces fresh stroma 400 ~ 600g.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. the method adopting Chinese medicinal herb nutrient liquid to cultivate Cordceps militaris, it is characterized in that: Radix Astragali 200g, Radix Angelicae Sinensis 150g, matrimony vine 150g, barrenwort 100g, cynomorium songaricum 100g, glossy ganoderma 100g, adding distil water 16000mL, after boiling in pot, put in drying baker, 80 DEG C of lixiviate 16h, are concentrated into 10000mL, obtain extracts of Chinese herbal medicine; In extracts of Chinese herbal medicine 2000mL, add glucose 20g, wheat bran 20g, rice meal 20 g, corn flour 20g, analysis for soybean powder 15g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, peptone 5g and 21 gold medals tie up him one, mix; Prepare medium according to a conventional method, bottling; 0.7kg/cm
2autoclaving, 115 DEG C of pressurize 60-90min; Inoculation, every bottle of inoculum concentration is 15%-20%; Adopt magnetic stirrer stir culture bacterium liquid, 22 DEG C of light culture 5-7 days; Then carry out bacterium liquid shallow-layer and cultivate Cordceps militaris.
2. employing Chinese medicinal herb nutrient liquid according to claim 1 cultivates the method for Cordceps militaris, it is characterized in that: described bacterium liquid shallow-layer is cultivated Cordceps militaris and comprised the following steps: bacterium liquid is sub-packed in culture bottle, culture bottle will be sterilized for subsequent use in advance, every bottled bacterium liquid height 1cm, 18 DEG C of light culture 7-8 days; Then h light cultivation every day 12 is proceeded to, intensity of illumination 200 lx, temperature 10 DEG C-20 DEG C, every temperature difference per day 10 DEG C; When scarlet caterpiller fungus mycelium becomes orange colour from white, and when starting to occur former base, regulate temperature and light photograph, temperature 18 DEG C-20 DEG C, whole day illumination cultivation, intensity of illumination 1000-1500 lx, humidity 80%-90%, air is fresh, gathers time ripe to stroma.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310671995.XA CN103621315B (en) | 2013-12-12 | 2013-12-12 | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310671995.XA CN103621315B (en) | 2013-12-12 | 2013-12-12 | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103621315A CN103621315A (en) | 2014-03-12 |
| CN103621315B true CN103621315B (en) | 2015-02-04 |
Family
ID=50203054
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310671995.XA Active CN103621315B (en) | 2013-12-12 | 2013-12-12 | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103621315B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087395A (en) * | 2015-08-27 | 2015-11-25 | 东北林业大学 | Method for culturing cordyceps militaris liquid spawn through magnetic stirring |
| CN105230336A (en) * | 2015-08-31 | 2016-01-13 | 佛山市高明区鹏鹄农业发展有限公司 | Method for planting cordyceps flower by microbe |
| CN106107974A (en) * | 2016-06-28 | 2016-11-16 | 黄秀英 | A kind of Fructus Jujubae, Fructus Lycii, Cordyceps combination mycelia block |
| CN107935643A (en) * | 2017-12-07 | 2018-04-20 | 米西道尔吉 | For cultivating nutrient solution containing cynomorium songaricum nutritional ingredient crop and preparation method thereof |
| CN109055239A (en) * | 2018-08-30 | 2018-12-21 | 重庆农笑农业发展有限公司 | Chinese caterpillar fungus bacterial filament fast culture process |
| CN110129168A (en) * | 2019-04-30 | 2019-08-16 | 李超飞 | A kind of Cordceps militaris prepares the culture medium prescription and preparation method of living Chinese caterpillar wine |
| CN111919665A (en) * | 2020-08-11 | 2020-11-13 | 南京康之春生物科技有限公司 | Cordyceps militaris cultivation inoculation method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100754A (en) * | 1993-09-24 | 1995-03-29 | 赵凤英 | Culture method of Cordyceps sinensis and its product |
| CN1935979A (en) * | 2006-11-01 | 2007-03-28 | 周永祥 | Coragyceps militaris bacterium culture method |
| CN101096641A (en) * | 2007-06-13 | 2008-01-02 | 杭州中美华东制药有限公司 | Culture medium for producing winter worm summer herb mycelium |
| KR100922311B1 (en) * | 2009-06-10 | 2009-10-21 | 이태봉 | Methods of culturing Inonotus obliquus, Phellinus linteus, Ganoderma lucidum, Sparassis crispa and Vegetable Worms for production of substances containing AHCC |
| TW200948371A (en) * | 2008-05-29 | 2009-12-01 | Jingwang Biotechnology Co Ltd | Cultivation method and liquid culture medium for cultivation of mycelium and fruiting body of Cordyceps |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
| CN102687853A (en) * | 2012-06-19 | 2012-09-26 | 福建农林大学 | Preparation method of cordyceps militaris bacterial powder and application thereof |
| CN102972201A (en) * | 2011-09-07 | 2013-03-20 | 严兴荣 | High-efficiency and fast cultivation technique of north worm grass |
-
2013
- 2013-12-12 CN CN201310671995.XA patent/CN103621315B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100754A (en) * | 1993-09-24 | 1995-03-29 | 赵凤英 | Culture method of Cordyceps sinensis and its product |
| CN1935979A (en) * | 2006-11-01 | 2007-03-28 | 周永祥 | Coragyceps militaris bacterium culture method |
| CN101096641A (en) * | 2007-06-13 | 2008-01-02 | 杭州中美华东制药有限公司 | Culture medium for producing winter worm summer herb mycelium |
| TW200948371A (en) * | 2008-05-29 | 2009-12-01 | Jingwang Biotechnology Co Ltd | Cultivation method and liquid culture medium for cultivation of mycelium and fruiting body of Cordyceps |
| KR100922311B1 (en) * | 2009-06-10 | 2009-10-21 | 이태봉 | Methods of culturing Inonotus obliquus, Phellinus linteus, Ganoderma lucidum, Sparassis crispa and Vegetable Worms for production of substances containing AHCC |
| CN102612985A (en) * | 2011-08-29 | 2012-08-01 | 何寒 | Production technology for cordyceps militaris mycelium |
| CN102972201A (en) * | 2011-09-07 | 2013-03-20 | 严兴荣 | High-efficiency and fast cultivation technique of north worm grass |
| CN102687853A (en) * | 2012-06-19 | 2012-09-26 | 福建农林大学 | Preparation method of cordyceps militaris bacterial powder and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 中草药提取液对冬虫夏草深层培养的影响;武忠伟等;《食品科学》;20050615;第26卷(第6期);第116-118页 * |
| 北冬虫夏草C-48生物学特性及栽培技术;张绪璋;《食用菌》;20031230;第12页 * |
| 北虫草新菌株选育及栽培技术研究;张绪璋;《中国农学通报》;20101005;第26卷(第19期);第206-210页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103621315A (en) | 2014-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103621315B (en) | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution | |
| CN104187947B (en) | A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. liquid fermenting beverage and its preparation method | |
| CN101760478B (en) | Preparation method of radix puerariae red yeast rice | |
| CN102746974B (en) | Cordyceps sinensis health wine and preparation method thereof | |
| CN102424625B (en) | Edible and medical fungi blackening agent, its preparation method and application | |
| CN107629965A (en) | A kind of preparation method of dendrobium candidum fungi fermentation product and its fermented product and application | |
| CN103583236A (en) | Method for preparing fleckedflesh polypore mycelium | |
| CN105219598A (en) | A kind of health care Cordyceps fungus wine and preparation method thereof | |
| CN102286339B (en) | Method for producing enzymatic rice bran red koji wine | |
| CN102771768A (en) | Morel health product and preparation method thereof | |
| TWI422680B (en) | Culture medium for culturing fruiting bodies of antrodia cinnamomea and method for culturing the same | |
| CN102771766A (en) | Tricholoma matsutake health-care product containing traditional Chinese medicine extractives and preparation method of tricholoma matsutake health-care product containing traditional Chinese medicine extractives | |
| CN106107974A (en) | A kind of Fructus Jujubae, Fructus Lycii, Cordyceps combination mycelia block | |
| CN106071573A (en) | It is a kind of that with Fructus Lycii and jujube juice, the Phellinus igniarius (L. ex Fr.) Quel. liquid spawn as carrier converts beverage | |
| CN103688748B (en) | Epimedium cordyceps militaris cultivation and product processing method | |
| CN106107943A (en) | Phellinus liteus block is prepared for culture medium with Fructus Jujubae and Fructus Lycii | |
| CN103897988B (en) | Periplaneta americana is utilized to cultivate the method for Cordyceps | |
| CN106036330A (en) | Lucid ganoderma liquid strain converted beverage taking wolfberry and red date juice as carriers | |
| KR101385234B1 (en) | Manufacturing Method of Fermented Materials for liver function improvement using Acanthopanax sp. | |
| CN105962321A (en) | Preparation method of lucid ganoderma fermentation solution | |
| CN106071587A (en) | With Herba Dendrobii juice for a kind of Cordyceps biological drink of carrier preparation | |
| CN102771769B (en) | Ganoderma sinensis health product and preparation method thereof | |
| CN103583229A (en) | Method for producing lucid ganoderma and lucid ganoderma tea by taking leguminous traditional Chinese medicine as raw materials | |
| CN102771858A (en) | Ganoderma sinensis health drink prepared by liquid-submerged fermentation and preparation method of Ganoderma sinensis health drink | |
| CN106047723A (en) | Preparation of phellinus linteus mycelia by taking red dates and wolfberry fruits as culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200119 Address after: No. 198, Qianlong street, Qianlong village, Jixiang Township, Gutian County, Ningde City, Fujian Province Patentee after: Fujian Gutian Nutritional Food Co., Ltd. Address before: Cangshan District of Fuzhou City, Fujian province 350002 to build a new town, Jinshan District Patentee before: Fujian Agricultural and Forestry University |