CN1100754A - Culture method of Cordyceps sinensis and its product - Google Patents
Culture method of Cordyceps sinensis and its product Download PDFInfo
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- CN1100754A CN1100754A CN 93117581 CN93117581A CN1100754A CN 1100754 A CN1100754 A CN 1100754A CN 93117581 CN93117581 CN 93117581 CN 93117581 A CN93117581 A CN 93117581A CN 1100754 A CN1100754 A CN 1100754A
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a new strain of northeast cordyceps, a culture method of the strain and a method for culturing the northeast cordyceps rich in zinc, germanium and selenium by using the strain, and extracting solution is prepared from the cultured northeast cordyceps, and the extracting solution, male silkworm moth, pit viper and the like are prepared into Tianwangbao nutriment.
Description
The invention belongs to northeast Chinese caterpillar fungus (Cordyceps militaris(L.ex.Fr) Link mandshurcum) cultivation of DBC-888 bacterial strain and extracting solution processing thereof and prepare nourishing liquid, oral liquid field with this extracting solution.
Existing at present much is the nutritious prod that raw material is made with the edible fungus, eat oral liquid as the TAIYANGSHEN hedgehog hydnum, the nutritive medium (CN88103922) that edible mushrooms is made through the fermentation of saccharic acid enzyme process, cordyceps sonensis oral liquid (CN92109862.6), wherein CN92109862.6 is the active substance that extracts in the Cordyceps militaris, make oral liquid, but its used Cordyceps militaris productive rate is very low.Effect is also not satisfactory.
The northeast Chinese caterpillar fungus originates in the Northeast China, belongs to together with Cordyceps sinensis.
The inventor is to the existing several years of the research of northeast Chinese caterpillar fungus, and in the screening of superior strain, aspects such as the booth production of northeast Chinese caterpillar fungus have obtained breakthrough, and to the application of northeast Chinese caterpillar fungus, the preparation aspect of healthcare products also has one's own knack.
The object of the present invention is to provide the new bacterial strain of northeast Chinese caterpillar fungus of cultivation.
Another object of the present invention is to provide the method for cultivating described new bacterial strain.
It is that main raw material is equipped with the healthcare products such as the precious senior tonic solution of the king with anticancer health care effect that Chinese medicines such as male Bombycis mori, pallas pit viper are made that one of purpose of the present invention provides the rich selenium of cultivating out with special methods, rich germanium, rich zinc type northeast Chinese caterpillar fungus.
Another object of the present invention is to provide the method for the above-mentioned northeast of preparation Chinese caterpillar fungus health product.
Wild northeast Chinese caterpillar fungus originates in northeast, parasitizes on the larva or pupa of lepidopterous insects, grows one or more sporophores (stroma) summer and autumn.The sporophore head is bar-shaped, and is leaf, and longitudinal furrow is arranged, orange-yellow, for can pregnant part.Shank is thin slightly, and color is also lighter, and sporophore total length 15-80mm, diameter are 2-4mm, and sporophore top (last 2/3 place) has the perithecium of ellipse garden shape, and thecaspore is arranged in the shell, emits from capsule when ripe.
As everyone knows, organic zinc, germanium, selenium are having significant effect aspect anticancer, health care, the raising body immunity, and inorganic zinc, selenium, germanium can not be absorption of human body, even it is also very low perhaps to absorb its transformation efficiency.The inventor after deliberation after, turn out than wild northeast Chinese caterpillar fungus output height, and the content of nutrition also is higher than the new bacterial strain of wild northeast Chinese caterpillar fungus.
One .DBC-888 bacterial strain
New bacterial strain (Cordyceps militaris(L.ex.Fr) Link.mandshurcum DBC-888) is the preferred of process wild state, strain separating, rejuvenation, directive breeding are preferably come out, it grows a plurality of sporophores on silkworm chrysalis, it is bar-shaped that sporophore is, expand the upper end, for orange-yellow, the lower end is thin slightly, paler colour.The high 50-100mm of sporophore, diameter 3-5mm cultivates containing on the rice medium of special nutrition, each Cans growth 20-30 strain, the high 60-130mm of sporophore, diameter 5-9mm, whole sporophore is orange, and the base portion color is light slightly.3/5 place of sporophore begins to expand, and the head garden is blunt, and is slightly sagging.
The characteristics of DBC-888 bacterial strain are:
1. output height.Each Cans mean yield is 20-35 gram (general bacterial strain, every Cans mean yield are the 5-10 gram).
2. contamination resistance is strong, and (several ten thousand bottles every batch) pollution rate is 10-25% in scale operation, and the pollution rate of general bacterial strain is 30-50%.
3. adaptability is strong, and temperature resistant range is wide.Mycelia and sporophore are all not dead at 30 ℃ to-20 ℃.
4. variability is good: by changing the composition of substratum, add the trace element (zinc, germanium, selenium) of different concns in substratum, can get rich zinc, germanium, selenium type Chinese caterpillar fungus.And this rich zinc, germanium, selenium effect are not machinery absorption but a kind of plysiochemical variation, and promptly by mycelium, sporophore absorbs, transform and be enriched in the resin of cell, protein, youngster's fourth matter, in polysaccharide and the nucleic acid, be about to inorganic elements and become organism, make it to be easy to be absorption of human body.
The northeast Chinese caterpillar fungus DBC-888 that the present invention cultivates contains 18 seed amino acids, the trace element of 30 multiple beneficial (as zinc, selenium, germanium, manganese, iron, calcium, phosphorus, potassium etc.) cordycepin, N.F,USP MANNITOL, Cordyceps polysaccharide, SOD, multivitamin, oleic acid, linolic acid, carotene etc.
Two. the present invention cultivates the method for northeast Chinese caterpillar fungus DBC-888:
(1) seed culture
1. first class inoculum is cultivated.
Former bacterial classification: the inventor from Benxi, areas of Shenyang gathers wild northeast Chinese caterpillar fungus, and with its separation, preferred fixed kind and it.
The prescription of first class inoculum substratum (calculating by 100% weight): Succus Rhizoma Dioscoreae 10%, saltpetre 0.5%, potassium primary phosphate 0.8%, VITAMIN liquid 0.5-2%, ferrous sulfate 0.05%, sal epsom 0.6%, glucose 2%, zinc sulfate 0.01%, Nucleotide 1%, agar 2%, milk 0.5%, all the other are water.
Be loaded on the substratum of above-mentioned preparation in vitro that Intake Quantity accounts for 1/5 of test tube volume, tampon beyond the Great Wall is through autoclaving (1Kg/cm
2Pressure) after 30 minutes, take out and to put into the inclined-plane, under aseptic condition, the mycelia of scraping the DBC-888 first class inoculum that takes a morsel with transfering loop was cultivated about 4 days in 22 ℃ of thermostat containers on the inclined-plane, and mycelia is covered with the inclined-plane.The feature of this bacterial classification is: mycelia is pure white, and is sturdy dense, and growth is rapid, later stage secretion xanthein.
2. second-class liquid isolate: use reciprocating type shaking table, 90 times/minute, amplitude 75mm.
Substratum contains (by 100% weight): Fructus Hordei Germinatus juice 2%, potassium primary phosphate 1%, soil extract 2%, sal epsom 0.6%, zinc sulfate 0.01%, ferrous sulfate 0.03%, aminoacids complex (commercially available) 0.5%, all the other are water.
Above-mentioned substratum is loaded in the 500-1000ml triangular flask, covers 8 layers of gauze, the outside puts polyester plastics and tightens, and carries out autoclaving (1Kg/cm
2) 30 minutes, after the cooling, with the DBC-888 first class inoculum, under the aseptic condition, be inoculated in the triangular flask, 1 test tube first class inoculum connects 5-10 triangular flask, build bottle cap (removing above-mentioned plastics adventitia), be placed on shaking table (reciprocating type shaking table, 90 times/minute, amplitude 75mm) on, under 20 ℃ of conditions, cultivated 5 days, there are a large amount of white bacterium balls to form in the triangular flask, nutrient solution is limpid, is good secondary bacterium liquid.(using) for the rich zinc of cultivation, rich selenium, rich germanium northeast Chinese caterpillar fungus.
(2) cultivation of .DBC-888 Cordyceps sporophore:
1. the rich zinc type of artificial culture northeast Chinese caterpillar fungus: substratum contains: (by 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, millet swill 2%, animal blood 1%, white squash seed meal 0.1-1%, Herba Epimedii 0.01-0.05%, all the other are soya-bean milk.Above-mentioned prescription is called for short nutritive medium.Get rice 40-50 gram, add in the clean Cans of packing into, above-mentioned nutritive medium 35-55 gram.360-900 μ g zinc additive is soluble in water, be added in the Cans, do lid with polyester film, tighten.(1.2Kg/cm in pressure kettle
2) sterilized 30 minutes, when dropping to 20 ℃ of left and right sides, temperature inoculates.
Inoculation with cultivate: will grow good DBC-888 second-class liquid isolate, with special Glass tubing inoculator, absorption 10ml under aseptic condition, receives in the above-mentioned Cans, builds polyester film again and puts to 15-20 ℃ of culturing room and cultivate.Through covering with white hypha on the substratum at Cans about a week, on the white hypha surface flavous liquid secretion thing was arranged on the Cans wall in 15-20 days.Grow fruit body primordium about one month, generally will grow the sporophore of orange in 40-60 days.After the sporophore maturation, under aseptic condition, adopt down with tweezers.Build and put back to culturing room, can grow second batch again through about 20 days.The zinc content of rich zinc Chinese caterpillar fungus is a 90-290 μ g/g(dry weight) than the content (30-80 μ g(dry weight) of the Chinese caterpillar fungus zinc of common artificial culture) improve 3-10 doubly.
2. the cultivation of rich selenium type northeast Chinese caterpillar fungus:
Substratum (nutritive medium) (by 100% weight).Potassium primary phosphate 1%, sal epsom 0.5-2%, VB
10.5-2%, animal blood 1%, all the other are soya-bean milk.
Getting rice 35-40 gram, Radix Astragali 0.5-3 gram packs in the clean Cans, Ensure Liquid liquid 35-45 gram, the selenium additive of 360-900 μ g also is added in the Cans after water-soluble again, builds lid, autoclaving, inoculation, cultivation (40-60 days grow stroma), collecting method are with rich zinc Chinese caterpillar fungus.The selenium content of this Chinese caterpillar fungus is 3-200 μ g/g, than high several times to hundred times of the Chinese caterpillar fungus selenium content (0.02-1 μ g/g) (dry weight) of common artificial culture.
3. the cultivation of rich germanium type northeast Chinese caterpillar fungus:
Nutritive medium (calculating) potassium primary phosphate 1%, sal epsom 0.5%, VB by 100% weight
10.5-2%, animal blood 1%, all the other are soya-bean milk.
Get rice 35-40 gram and add Ganoderma powder or join powder 0.2-1 gram in vain, Ensure Liquid liquid 35-50 restrains and gets in Cans in the water-soluble back adding of the germanium additive 360-900 μ g/g Cans again, builds lid.Autoclaving, inoculation, cultivation (40-60 days grow sporophore), collecting method are with rich zinc Chinese caterpillar fungus.The germanic amount of this rich germanium Chinese caterpillar fungus is a 1-160 μ g/g(dry weight) than common artificial culture Chinese caterpillar fungus, germanic amount 0.015-2 μ g/g(dry weight) high tens times.
4. on silkworm chrysalis, cultivate the method for rich zinc, rich germanium, rich selenium type northeast Chinese caterpillar fungus:
The silkworm chrysalis that body surface is disinfected is put in the Cans after the sterilization, and every bottled 5-10 pupa with the several holes of inoculation pinprick (3-5), is poured second-class liquid isolate 5ml in the Cans that fill silkworm chrysalis under aseptic condition then, builds polyester film, shakes up.Place 18-22 ℃, casual light is cultivated down, when treating that fruit body primordium forms, uses syringe, extracts 3ml, and the selenium for preparing in advance, germanium, the zinc aqueous solution (concentration is 4000ppm) spray to length to be had on the silkworm chrysalis of fruit body primordium, grows up to through 15 days left and right sides sporophores.Cultivate Chinese caterpillar fungus in this way, it contains the Chinese caterpillar fungus that zinc, germanium, selenium amount cultivates than general method and carries 3-15 doubly, and output improves 6-30%.
5. in booth, cultivate rich zinc, germanium, selenium type northeast Chinese caterpillar fungus
Because DBC-888 bacterial strain adaptive faculty and anti-infection ability are strong, can carry out big area and cultivate.Its method is as follows: under aseptic condition, getting DBC-888 secondary bacterium liquid 0.2ml is expelled in silkworm chrysalis (tussah, the silkworm) body that body surface disinfects, put into the Cans of the bacterium of going out with taking over the silkworm chrysalis of planting, Intake Quantity is 2/3rds of Cans, seal with polyester film, place Cans 20-25 ℃ to cultivate 7 days down.The pupa that to grow mycelia is again put into the compost of warmhouse booth, leaf on the upper cover, and the spacing of pupa is 50-70mm.The temperature of booth is controlled at 17-22 ℃, relative humidity 90-95%, regularly ventilation.Grew and the similar stroma of wild northeast Chinese caterpillar fungus through 15-25 days.Spray to stroma with the 4000ppm germanium, zinc, the selenium aqueous solution that prepare when stroma just is unearthed, after 10-15 days, the stroma maturation was in time gathered.
The rice for preparing nutritive medium can also be packed in polyester or the polypropylene plastics bag, through autoclaving, behind the inoculation DBC-888, place 20-25 ℃ to cultivate two weeks down, rice covers with mycelia, places the compost of above-mentioned condition warmhouse booth, also grows and the similar stroma of wild northeast worm in 25-40 days.Spray to stroma with the 4000ppm germanium, zinc, the selenium aqueous solution that prepare when stroma just is unearthed, after 10-15 days, the stroma maturation was in time gathered.Also can select to cultivate with aforesaid method in the soil of the hillside thick forest that suits.
Effective ingredient extracting method in the three .DBC-888 northeast Chinese caterpillar funguses:
For the extracting method of northeast Chinese caterpillar fungus, current still problem of studying generally all takes conventional alcohol immersion to extract the hot-water soak extracting method.The present invention is with the new fresh sporophore of northeast Chinese caterpillar fungus, grinds, and carries out press filtration with the deckle board strainer, filtrate is placed in the refrigerator and preserves, and filter residue is used ethanol again, and filtrate is extracted in hot-water soak, filtrate in this filtrate and the refrigerator is mixed into the Chinese caterpillar fungus extracting solution, and this extracting solution can be made into the healthcare products of various formulations.Also can cooperate and make health promoting wine, oral liquid, pulvis, health tea etc. with other herbal medicine.
Four. northeast cordyceps product and technology thereof
The extracting solution that the precious senior tonic solution of king among the present invention is exactly the northeast Chinese caterpillar fungus adds herbal medicine and makes.The precious senior tonic solution of king is according to motherland's medical treasure-house, promotes longevity, and the secret recipe of kidney invigorating and YANG supporting, the fresh DBC-888 Chinese caterpillar fungus that is equipped with a kind of special cultivation is a main raw material, adopts modern biotechnology to make with extra care and forms.This product integrates nourishing, keeps healthy, treats, and merit is the immunologic function of nourishing and strengthening vital, Ginseng Extract, raising human body, removes free radical, improves the anti-ageing ability of body.
Motherland's medical science is thought: the kidney being the origin of congenital constitution, kidney storing essence, kidney essense are the base substances of human development's reproduction, be called as human life this, the prosperity and decline of life often has direct relation with the function power of kidney.And the precious senior tonic solution of king effects a permanent cure with nourishing just.
The characteristics of the precious senior tonic solution of king:
(1) difference of the precious senior tonic solution of king and other kidney invigorating and YANG supporting medicine is that the nourishing Chinese medicine that the medicinal material selected for use in the prescription is preciousness belongs to pure biological products, the Chinese medicine that does not contain any side effect, do not contain pharmaceutical chemicals and artificial color and narcotic, to the without any side effects and bad reflection of human body, be the reliable nutrient health of non-toxic and safe, the anti-station toxicological test of establishing proves to human body it is safe and reliable through the Shenyang City.
(2) raw material sources conveniently are easy to get.
(3) collocation of holozoic nutrition and holophytic nutrition is reasonable in the prescription, and science has played the good action of having complementary advantages.
(4) the maximum characteristics of the precious senior tonic solution of king are that used northeast Chinese caterpillar fungus is the bright Chinese caterpillar fungus of cultivating with special methods in the prescription.
(5) the precious senior tonic solution nutrition of king is abundant and content is high, be general healthcare products can not compare.Wherein contain 18 seed amino acids, make the aspartic acid content of health Ginseng Extract reach 526mg/L, the L-glutamic acid content that increases brain cell activity is 397mg/L, the arginine of sexual function improvement is arranged, zinc, germanium, selenium etc.Cordycepin, Cordyceps polysaccharide, selenium, germanium etc. anticancer, anti-cancer are arranged, SOD, carotene, the V of anti-aging effects arranged
E, zinc etc., Vpp, oleic acid, the linolic acid of treatment, health care cardiovascular systems are arranged.Its content is auspicious sees laboratory test report.From nutrient contg, be 2-30 times of the famous long-lived permanently happy tonic wine in China and foreign countries.(auspicious see laboratory test report).
Embodiment 1: senior tonic solution is formed and blending process:
Form:
Genseng 1.5-6% Chinese caterpillar fungus extracting solution 0.5-5%
Radix Astragali 2-5% pallas pit viper 0.5-2%
Herba Epimedii 3-6% male Bombycis mori 1-5%
Radix Angelicae Sinensis 1-4% animal genital (donkey, dog, deer whip) 0.5-2%
Bark of eucommia 1-5% pilose antler 0.3-1%
Matrimony vine 2-5% desert cistanche 0.5-2%
Tuber Fleeceflower Root 1-5% cynomorium songaricum 1-5%(can be with Root of Medicinal Indian mulberry generation)
Spend soaking in Chinese liquor 3-6 after individual month by the 14 flavor Chinese medicines (except that the Chinese caterpillar fungus extracting solution of northeast) that above-mentioned (1) is formed with 60, filter and in filtrate, add northeast Chinese caterpillar fungus extracting solution 0.5-5% again, with rock sugar 8%, with seasonings 1% tonic solution.
Embodiment 2: the blending process of king's Bao oral liquid
Press the flavor Chinese medicine of 14 in the foregoing description 1 (except that the Chinese caterpillar fungus extracting solution of northeast) and spend liquor with 60, after soaking 3-6 month, filter, filtrate through distill concentrate remove ethanol after, add extracting solution 0.5-5%, rock sugar 8% that a certain amount of spring (water yield is suitable with the amount of alcohol of removing) adds the northeast Chinese caterpillar fungus again, king's Bao oral liquid.
Check:
Prepare the precious senior tonic solution of king, oral liquid, every index: nutritions such as amino acid, VITAMIN, trace element, cordycepin, SOD, Cordyceps polysaccharide, oleic acid, linolic acid, calabash radish element meet the company standard acceptance(check) through chemical examination.
Packing and sterilization:
After product is qualified, under aseptic condition,, be sub-packed in the bottle of clean different size oral liquid, tonic solution.Carry out sterilising treatment (with pasteurizer sterilization, 75-80 ℃ condition following 20 minutes) and carry out bacterium inspection, qualified back decals.
Store: oral liquid is preservation period half a year at low temperatures
Preservation period is 2 years under the senior tonic solution normal temperature.
Northeast Chinese caterpillar fungus DBC-888 bacterial classification has been preserved in BeiJing ZhongGuanCun China Committee for Culture Collection of Microorganisms common micro-organisms center on September 23rd, 1993.Preserving number: CGMCC NO.0200.
Claims (10)
1, a kind of northeast Chinese caterpillar fungus (Cordyceps militaris (L.ex.Fr) Linkmandshurcum) DBC-888 bacterial strain.
2, the northeast Chinese caterpillar fungus of claim 1 is made the method for secondary bacterium liquid, it is characterized in that: the prescription of the substratum of first class inoculum: (calculating by 100% weight) Succus Rhizoma Dioscoreae, saltpetre 0.5%, potassium primary phosphate 0.8%, VITAMIN liquid 0.5-2%, ferrous sulfate 0.05%, sal epsom 0.6%, glucose 2%, zinc sulfate 0.01%, Nucleotide 1%, agar 2%, milk are a small amount of, and all the other are water.
The substratum of above-mentioned preparation is loaded in vitro, and Intake Quantity accounts for 1/5 of test tube volume, and tampon beyond the Great Wall is through autoclaving (1Kg/cm
2Pressure) after 30 minutes, take out and to put into the inclined-plane, under aseptic condition, the mycelia of northeast Chinese caterpillar fungus of scraping the claim 1 that takes a morsel with transfering loop was cultivated about 4 days in 22 ℃ of thermostat containers on the inclined-plane, and mycelia is covered with the inclined-plane.The feature of this bacterial classification is: mycelia is pure white, and is sturdy dense, and growth is rapid, later stage secretion xanthein.
Second-class liquid isolate is cultivated: use the shaking table shaking culture.
Substratum contains (by 100% weight) Fructus Hordei Germinatus juice, potassium primary phosphate 1%, sal epsom 0.6%, zinc sulfate 0.1%, ferrous sulfate 0.03%, aminoacids complex 0.5%, a small amount of, the soil extract of fresh milk, and all the other are water.
Above-mentioned substratum is loaded in the 500-1000ml triangular flask, covers 8 layers of gauze, the outside puts polyester plastics and tightens, and carries out autoclaving (1Kg/cm
2) 30 minutes, after the cooling, with the DBC-888 first class inoculum, under the aseptic condition, be inoculated in the triangular flask, 1 test tube first class inoculum connects 5-10 triangular flask, build bottle cap, remove above-mentioned plastics adventitia, be placed on the shaking table, under 20 ℃ of conditions, cultivated 5 days, have a large amount of white bacterium balls to form in the triangular flask, nutrient solution is limpid, is good secondary bacterium liquid.
3, cultivate the method for rich zinc type northeast Chinese caterpillar fungus, it is characterized in that: substratum, or claim nutritive medium (by 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, little water in which rice has been washed, animal blood, all the other are soya-bean milk;
Get rice 40-50g, white squash seed meal, Herba Epimedii, put into clean Cans, add above-mentioned nutritive medium, with the 35-45 gram, in the Cans of packing into, zinc additive is water-soluble again, adds in the Cans, does lid with polyester film, tightens autoclaving (1.2Kg/cm
2) 30 minutes, inoculation when temperature drops to 20 ℃;
The northeast Chinese caterpillar fungus second-class liquid isolate of long claim 2 is well drawn 10ml with special Glass tubing inoculator, under aseptic condition, be inoculated in the above-mentioned Cans, build polyester film again, put to 15-20 ℃ of culturing room and cultivate, generally will grow the northeast Chinese caterpillar fungus of orange in 40-60 days.
4, cultivate the method that contains selenium type northeast Chinese caterpillar fungus, it is characterized in that substratum or nutritive medium (in 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, VB
10.5%, animal blood, all the other are soya-bean milk, getting rice 35-40 gram, the Radix Astragali packs into and adds above-mentioned nutritive medium 35-45 gram in the clean Cans, pack in the Cans, again the selenium additive also is added in the Cans after water-soluble, seals bottleneck with polyester film, autoclaving, reduce to normal temperature, the northeast Chinese caterpillar fungus second class inoculum of claim 2 is inoculated in the Cans, places 17-20 ℃ to cultivate 40-60 days, grow stroma.
5, cultivate the method for germanic type northeast Chinese caterpillar fungus, it is characterized in that substratum or nutritive medium (in 100% weight): potassium primary phosphate 1%, sal epsom 0.5%, VB
10.5%, animal blood, all the other are soya-bean milk, get rice 35-40 gram, add Ganoderma powder or join powder in vain, Ensure Liquid liquid 35-50 restrains in Cans, gets the water-soluble back of germanium additive again and adds in the Cans, seal mouth with polyester film, autoclaving is reduced to the normal temperature inoculation, by the northeast Chinese caterpillar fungus second class inoculum inoculation of claim 2, cultivated 40-60 days, and grew sporophore.
6, on silkworm chrysalis, cultivate the method for rich zinc, germanium, selenium type northeast Chinese caterpillar fungus, it is characterized in that: the silkworm chrysalis that body surface is disinfected is put in the Cans after the sterilization, every bottled 5-10 pupa, under aseptic condition, with inoculation pinprick, 3～5 holes, the northeast Chinese caterpillar fungus second-class liquid isolate 5ml with claim 2 pours in the Cans that fill silkworm chrysalis then, build polyester film, shake up.Place 18-22 ℃, the light that overflows is cultivated down, when treating that fruit body primordium forms, uses syringe, extracts 3ml, the selenium for preparing in advance, germanium, the zinc aqueous solution.Spraying to length has on the silkworm chrysalis of fruit body primordium.Through about 15 days, promptly grow up to Cordyceps sporophore.
7, in warmhouse booth, cultivate the method for rich zinc, germanium, selenium type northeast Chinese caterpillar fungus, it is characterized in that: under aseptic condition, the weighting profit requires 2 northeast Chinese caterpillar fungus secondary bacterium liquid 0.2ml to be expelled to the silkworm chrysalis that body surface disinfects, in tussah, silkworm body, put into the Cans of the bacterium of going out with taking over the silkworm chrysalis of planting, Intake Quantity is 2/3rds of Cans, uses the polypropylene film seal, places Cans 20-25 ℃ to cultivate 7 days down.The pupa that to grow mycelia is again put into the compost of warmhouse booth, leaf on the upper cover, and the spacing of pupa is the 50-70 millimeter.The temperature of booth is controlled at 17-20 ℃, relative humidity 90-95%, regularly ventilation.When stroma just was unearthed, the aqueous solution of the zinc germanium that usefulness prepares, germanium, selenium was sprayed on the stroma.Process grew stroma in 15-25 days.
8, extract the method for northeast Chinese caterpillar fungus effective ingredient, it is characterized in that, the sporophore of claim 3-7 is ground, with the press filtration of deckle board strainer, filtrate is placed in the refrigerator and preserves, and filter residue is used ethanol again, hot-water soak, extract filtrate, it is mixed with the filtrate in the refrigerator, become northeast Chinese caterpillar fungus extracting solution.
9, the precious senior tonic solution of a kind of king, it is characterized in that: (by 100% restatement) is with genseng 1.5-6%, Radix Astragali 2-5%, pallas pit viper 0.5-2%, Herba Epimedii 3-6%, male Bombycis mori 1-5%, Radix Angelicae Sinensis 1-4%, animal genital, as donkey, dog, deer 0.5-2%, bark of eucommia 1-5%, pilose antler 0.3-1%, matrimony vine 2-5%, desert cistanche 0.5-2%, Tuber Fleeceflower Root 1-5%, cynomorium songaricum 1-5%, or replace with Root of Medicinal Indian mulberry, all the other are wine, spend soaking in Chinese liquor 3-6 after individual month with 60, filter to such an extent that vina adds 0.5-5% claim 8 northeast Chinese caterpillar fungus extracting solution, add 8% rock sugar, with seasonings 1%.
10, a kind of blending process of king's Bao oral liquid, it is characterized in that (by 100% weight) general: genseng 1.5-6%, Radix Astragali 2-5%, pallas pit viper 0.5-2%, Herba Epimedii 3-6%, male Bombycis mori 1-5%, Radix Angelicae Sinensis 1-4%, animal genital, as donkey, dog, deer 0.5-2%, bark of eucommia 1-5%, pilose antler 0.3-1%, matrimony vine 2-5%, desert cistanche 0.5-2%, Tuber Fleeceflower Root 1-5%, cynomorium songaricum 1-5%, or replace with Root of Medicinal Indian mulberry, after 60 ° of soaking in Chinese liquor 3-6 months, filter, filtrate through distill concentrate remove ethanol after, add a certain amount of spring, the water yield is suitable with the amount of alcohol of removing, add right again and require 8 northeast Chinese caterpillar fungus extracting solution 0.5-5%, rock sugar 8%.
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| CN93117581A CN1091149C (en) | 1993-09-24 | 1993-09-24 | Culture method of Cordyceps sinensis and its product |
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| CN1094754A (en) * | 1993-09-12 | 1994-11-09 | 李江 | Artificial cultivation method of selenium-rich Cordyceps militaris |
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| CN103621315B (en) * | 2013-12-12 | 2015-02-04 | 福建农林大学 | Method for cultivating cordyceps militaris by using Chinese herbal medicine nutrient solution |
| CN104756759A (en) * | 2015-03-17 | 2015-07-08 | 沁阳市西向食用菌研究所 | Dioscorea opposita cordyceps sinensis and culture method thereof |
| CN105746172A (en) * | 2016-02-23 | 2016-07-13 | 张家港顺泰元生物科技有限公司 | Selenium-enriched cordyceps culture method |
| CN106434380A (en) * | 2016-10-14 | 2017-02-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordyceps sinensis by utilizing astragalus membranaceus and application thereof |
| CN106520562A (en) * | 2016-10-14 | 2017-03-22 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for cultivating caterpillar fungus by using ginseng type extract and application |
| CN107960270A (en) * | 2017-08-31 | 2018-04-27 | 广西沙田仙人滩农业投资有限公司 | A kind of method of cordyceps militaris cultivation |
| CN112166954A (en) * | 2020-10-27 | 2021-01-05 | 苏州家和蚕业生物科技有限公司 | Culture method of cordyceps militaris |
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