CN102771766A - Tricholoma matsutake health-care product containing traditional Chinese medicine extractives and preparation method of tricholoma matsutake health-care product containing traditional Chinese medicine extractives - Google Patents
Tricholoma matsutake health-care product containing traditional Chinese medicine extractives and preparation method of tricholoma matsutake health-care product containing traditional Chinese medicine extractives Download PDFInfo
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- CN102771766A CN102771766A CN2012102545189A CN201210254518A CN102771766A CN 102771766 A CN102771766 A CN 102771766A CN 2012102545189 A CN2012102545189 A CN 2012102545189A CN 201210254518 A CN201210254518 A CN 201210254518A CN 102771766 A CN102771766 A CN 102771766A
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Abstract
The invention relates to a tricholoma matsutake health-care product containing traditional Chinese medicine extractives and a preparation method of tricholoma matsutake health-care product containing the traditional Chinese medicine extractives. The preparation method provided by the invention comprises the following steps of: A, adding tricholoma matsutake in a nutrient solution and obtaining a culture solution through shaking culture on a shaker; B, adding the culture solution obtained in the step A in a liquid nutrient medium containing barley malt and soybeans and obtaining a fermented tricholoma matsutake mycelium and tricholoma matsutake fermented broth through aerobic culture; C, extracting a tricholoma matsutake mycelium solution by water after the tricholoma matsutake mycelium is subjected to cell tissue crushing; D, mixing the tricholoma matsutake fermented broth obtained in the step B and the tricholoma matsutake extracting solution obtained in the step C, diluting or concentrating the mixed liquor and then obtaining a tricholoma matsutake health-care product raw material; and E, adding the traditional Chinese medicine extractives in the tricholoma matsutake health-care product raw material obtained in the step D. According to the invention, the tricholoma matsutake health-care product containing the traditional Chinese medicine extractives can be applicable to mass production in an industrialized way, so that the process is simple and the cost is controllable.
Description
Technical field
The present invention relates to a kind of health products and preparation method thereof, particularly relate to a kind of matsutake health-care article that contain Chinese medical extract and preparation method thereof.
Background technology
Matsutake [formal name used at school: Tricholoma matsutake (Lto et lmai) singer etc.] has another name called Trichotoma matsutake, is famous and precious edible mushroom.Matsutake is rich in protein, fat, cellulose, several amino acids, insatiable hunger and closes aliphatic acid; Also contain rare β in other mushroom-glucose acid, nucleic acid derivative, peptide matters; Organic germanium Ge124-239PM, rare nutrients such as polysaccharide LPB have the SOD of raising activity; Quicken the removing of free radical, delay the histoorgan decline, improve cardiovascular function; Enhance metabolism, the raising human body is antiviral, anti-cell suddenlys change and the ability of increase immunologic function.Normal food matsutake can strong smart kidney tonifying, regains one's vigor, and other has beneficial stomach tonifying Qi, and cardiac stimulant is enriched blood, brain tonic and intelligence development, regulating qi-flowing for eliminating phlegm, expelling parasite and control diabetes and effect such as anticancer." Sichuan gill fungus bacterium " secretary is carried, " according to Japan thousand former Wu youths, its hot water extract is to small white mouse sarcoma S---and 180 inhibiting rates are 91.8%, are 70% to its Emhorn knurl inhibiting rate." matsutake is a kind of rare famous and precious edible fungi of pure natural, is described as " king in the bacterium ".After passing on from one to another in August, 1945 hiroshima bomb attack, the plant of unique survival has only matsutake.
At present, correlative study both domestic and external mainly concentrates on extraction and pharmacology, the drug effect of matsutake function factor.But the artificial cultivation field at Tricholoma matsutake (lto et lmai) Singer sporophore but receives a lot of technical limitations, carries out suitability for industrialized production and civil nature use widely seldom on a large scale, and simultaneously, this technical field also is difficult to commercialization and uses.Before 10 years, the year recoverable amount of occurring in nature Tricholoma matsutake (lto et lmai) Singer sporophore has 10,000 tons approximately, 5 kilotons but present recoverable amount is but only had an appointment; And; As stated, Tricholoma matsutake (lto et lmai) Singer sporophore is difficult to a large amount of manual work production again owing to technical reason; Therefore, people have produced significant contradiction to the shortage of craving for the Tricholoma matsutake (lto et lmai) Singer sporophore recoverable amount of matsutake health-care effect and therapeutic efficiency.
Summary of the invention
The objective of the invention is to; Overcome the defective of above-mentioned prior art and a kind of suitability for industrialized production of can carrying out on a large scale is provided, and can produce the matsutake health-care article preparation method who contains Chinese medical extract of the higher Tricholoma matsutake (lto et lmai) Singer sporophore of effectiveness with lower cost.
For realizing above-mentioned purpose, the present invention takes following design:
A kind of matsutake health-care article preparation method who contains Chinese medical extract of the present invention; Comprise the steps: A, the matsutake bacterium is inserted in the nutrient solution; And under 18～28 ℃ temperature, on shaking table, shake cultivation 72～96 hours with 200～300 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the tricholoma matsutake mycelium extract; D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, dilute or concentrate, obtain matsutake health-care article raw material; In E, the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
Preferably; In said step e, add in the following Chinese medical extract account for matsutake health-care article parts by weight of raw materials one or more: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.
Preferably, in said step D, water or mycelium extract perhaps utilize 2～5 times of said mixed liquor dilutions the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
Preferably, in said step D, the mutual mixed pH value of matsutake fermented liquid and tricholoma matsutake mycelium extract is 6～7, and the refractive power pol is 2～6%.
Another object of the present invention is to provide a kind of matsutake health-care article that contain Chinese medical extract, said matsutake health-care article are to be prepared from following method:
A, the matsutake bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the tricholoma matsutake mycelium extract; D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, dilute or concentrate, obtain matsutake health-care article raw material; In E, the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
Preferably; In said step e, add in the following Chinese medical extract account for matsutake health-care article parts by weight of raw materials one or more: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.
Preferably, in said step D, water or mycelium extract perhaps utilize 2～5 times of said mixed liquor dilutions the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
Advantage of the present invention is: the present invention adopts that the matsutake bacterium in bacterial classification original producton location separates, purifying and cultivation; Adopt industrialization liquid deep layer fermenting technology, make the matsutake bacterial classification to carry out a large amount of breedings in the liquid medium within, and; All contain in the metabolite of gained and the mycelium and the identical active ingredient of former matsutake bacterium effectiveness; In addition, the present invention also makes the fructification of matsutake bacterium to produce and to cultivate in industrialized condition, and technology is simple, cost is controlled; Thereby can reduce production costs significantly, make ordinary consumer also can use famous and precious health product.
The specific embodiment
Embodiment 1:
Following is a kind of preferred embodiment that contains the matsutake health-care article preparation method of Chinese medical extract of the present invention, and preparation method of the present invention comprises following key step:
A, at first; The matsutake bacterium is inserted in the nutrient solution; Nutrient solution of the present invention can be the various nutrient solutions that are suitable for the cultivation of matsutake bacteria liquid of the prior art, and under 18 ℃ temperature, on shaking table, shakes cultivation 72 hours with 300 times/minute concussion frequency, obtains strain liquid; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 8% fructus hordei germinatus, weight portion 6% soybean, and inoculum concentration is 5%, and ventilation was cultivated 48 hours under 27 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, again the tricholoma matsutake mycelium that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.8:5, extraction temperature is 95 ℃, extraction time is 2 hours, thereby obtains the tricholoma matsutake mycelium extract; Further, matsutake zymotic fluid and wet thallus that the present invention mainly utilizes industrialization liquid deep layer fermenting technology to obtain prepare and produce matsutake health-care article or health drink; D, afterwards after the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other, dilutes or concentrates, and obtains matsutake health-care article raw material; In the present embodiment, be preferably the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, concentrate 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain matsutake health-care article raw material; E, last, in the matsutake health-care article raw material that in said step D, obtains, adding Chinese medical extract, said Chinese medicine comprise Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.Add Chinese yam, Radix Glycyrrhizae and fruit of Chinese wolfberry extract formation mixture separately in the present embodiment, and add honey.
Certainly, to those skilled in the art, be appreciated that the extract that can also add other several kinds of Chinese medicines in the above-mentioned Chinese medicine, and process thus and have the matsutake health-care article of comprehensive health-care curative effect more.
And, through preparation method of the present invention, can produce popular straight drink product, comprise health products and beverage.And preparation method of the present invention can directly apply to suitability for industrialized production, and cost is low, and the products obtained therefrom trophic function is high, helps consumer and public daily health carings.
Particularly; In step e of the present invention, add in the following Chinese medical extract account for matsutake health-care article parts by weight of raw materials one or more: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.In the present embodiment, the yam extract of 0.3% weight portion, the licorice of 0.8% weight portion, the Fructus lycii P.E of 0.5% weight portion and the honey of 0.8% weight portion have been added.Certainly, also can add the yam extract of 0.3% weight portion separately, or add the Fructus lycii P.E of 0.5% weight portion separately, or add the honey of 0.8% weight portion separately.
Need to prove in the above-mentioned Chinese medicine that except that honey, other traditional Chinese medicinal components all join in the said health products raw material with the form of extract, and can add a kind of Chinese medical extract separately, also can add wherein several kinds or whole mixtures of Chinese medical extracts.The content of the Chinese medical extract that adds also can select to account for the various ratios in said health products raw material 0.3～0.8% weight portion interval according to the production needs.
Through the foregoing description, preparation method of the present invention can directly carry out industrial and large-scale and produce continuously.After the batch process, can produce the matsutake fermented liquid more than 10 tons every day, and produce 20 tons～final products more than 50 tons every day through preparation method of the present invention.
And the method for concentration that in present embodiment preparation method's step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration.Preferably, in the present embodiment, adopt the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained matsutake health-care article raw material, the content of its functional components and Tricholoma matsutake (lto et lmai) Singer sporophore are close or be higher than Tricholoma matsutake (lto et lmai) Singer sporophore, and its effectiveness is with directly to take matsutake suitable.
More preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) are preferably and reach relevant parameters separately simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed natural pH value of matsutake fermented liquid and tricholoma matsutake mycelium extract is 6～7, and the refractive power pol is 2～6%.
The content of functional component of the inventive method products therefrom and quality are all highly stable, and are higher than modern pharmacology and research and analyse the numerical value scope of determination to natural Cordyceps militaris component content.Preparation method's of the present invention industrialization control ability is strong, and its cost is far below natural material.
Embodiment 2:
Preparation method's of the present invention another kind of preferred embodiment will be provided as follows.The preparation method of present embodiment comprises: A, at first, select food, medicinal bacterium matsutake for use, and the matsutake bacterium is inserted in the nutrient solution, and under 28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 96 hours with 200 times/minute concussion frequency; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 12% fructus hordei germinatus, weight portion 3% soybean, and inoculum concentration is 10%, and ventilation was cultivated 72 hours under 23 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, again the tricholoma matsutake mycelium that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 1:2, extraction temperature is 90 ℃, extraction time is 3 hours, thereby obtains the tricholoma matsutake mycelium extract; D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, dilute, be preferably water or mycelium extract with 2～5 times of said mixed liquor dilutions, thereby obtain matsutake health-care article raw material; E, last, in the matsutake health-care article raw material that in said step D, obtains, adding Chinese medical extract, said Chinese medicine comprise Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.Add lily, radix polygonati officinalis, Poria cocos and root of kudzu vine extract formation mixture separately in the present embodiment, and add honey.Particularly, the Bulbus Lilii extract of 0.4% weight portion, the Rhizoma Polygonati Odorati extract of 0.6% weight portion, the tuckahoe extracts of 0.5% weight portion, the kudzu root extract of 0.7% weight portion and the honey of 0.3% weight portion have been added.
Embodiment 3:
Another preferred embodiment of preparation method of the present invention will be provided as follows.The preparation method of present embodiment comprises the steps: A, the matsutake bacterium is inserted in the nutrient solution, and under 22 ℃ temperature, on shaking table, shakes cultivation 80 hours with 260 times/minute concussion frequency, obtains strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 10% fructus hordei germinatus, weight portion 5% soybean; Inoculum concentration is 7%; And ventilation was cultivated 60 hours under 25 ℃ temperature, through biofermentation, obtained the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.9:4, extraction temperature is 93 ℃, extraction time is 2.5 hours, thereby obtains the tricholoma matsutake mycelium extract; D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after; The mixed liquor of gained is concentrated 6 times, and make its refractive power pol reach 10%, thereby obtain the matsutake health-care raw material; E, last; In the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.Certainly, also can add sugar or sweetener as required.
Embodiment 4:
Present embodiment provides a kind of matsutake health-care article that contain Chinese medical extract; Said matsutake health-care article are to be prepared from following method: A, will select for use food, medicinal matsutake bacterium to insert in the nutrient solution; And under 18～28 ℃ temperature (present embodiment is preferably 20 ℃), on shaking table, shake 72～96 hours (present embodiment is preferably 82 hours) of cultivation with the concussion frequency of 200～300 times/minute (present embodiment is preferably 260 times/minute), obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% (present embodiment is preferably 11%) fructus hordei germinatus, weight portion 3～6% (present embodiment is preferably 5%) soybean; Inoculum concentration is 5～10% (present embodiment is preferably 8%); And ventilation is cultivated 48～72 hours (present embodiment is preferably 56 hours under the temperature of 23～27 ℃ (present embodiment is preferably 26 ℃); Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation; C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after; Water extracts; The lixiviate ratio is 0.8～1:2～5 (present embodiment is preferably 1:3); Extraction temperature is 90～95 ℃ (present embodiment is preferably 92 ℃), and extraction time is 2.5 hours, thereby obtains the tricholoma matsutake mycelium extract; D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after; The mixed liquor of gained is diluted 2～5 times or concentrated 4～7 times; In the process that concentrates, make its refractive power pol reach 10～12%, thereby obtain the matsutake health-care raw material; E, last; In the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.Add Chinese yam, lily, the fruit of Chinese wolfberry and root of kudzu vine extract formation mixture separately in the present embodiment, and add honey.Particularly, the yam extract of 0.3% weight portion, the Bulbus Lilii extract of 0.5% weight portion, the Fructus lycii P.E of 0.4% weight portion, the kudzu root extract of 0.7% weight portion and the honey of 0.6% weight portion have been added.
Wherein, in step D, when adopting concentration process, the method for concentration that is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained matsutake health-care article, its effectiveness is with directly to take matsutake suitable.
Preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) reach corresponding separately index simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed pH value of matsutake fermented liquid and tricholoma matsutake mycelium extract is 6～7, and the refractive power pol is 2～6%.
The present invention is a kind of, and to contain other embodiment of matsutake health-care article of Chinese medical extract identical or close with the foregoing description 1,2 and 3, repeats no more at this.To those skilled in the art, be not difficult from the foregoing description 1,2 and 3, to obtain relevant teachings, and in order to preparation or production matsutake health-care article.
Obviously, those of ordinary skill in the art can form various types of health products and preparation method thereof with a kind of logical matsutake health-care article preparation method who contains Chinese medical extract of the present invention.
The foregoing description only supplies to explain the present invention's usefulness; And be not to be limitation of the present invention; The those of ordinary skill in relevant technologies field without departing from the present invention, can also be made various variations and modification; Therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited each claim.
Claims (8)
1. matsutake health-care article preparation method who contains Chinese medical extract, it is characterized in that: said preparation method comprises the steps:
A, the matsutake bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation;
C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the tricholoma matsutake mycelium extract;
D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, dilute or concentrate, obtain matsutake health-care article raw material;
In E, the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
2. a kind of matsutake health-care article preparation method who contains Chinese medical extract according to claim 1; It is characterized in that: in said step e, add in the following Chinese medical extract account for matsutake health-care article parts by weight of raw materials one or more: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.
3. a kind of matsutake health-care article preparation method who contains Chinese medical extract according to claim 1 and 2; It is characterized in that: in said step D; Water or mycelium extract perhaps utilize 2～5 times of said mixed liquor dilutions the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
4. a kind of matsutake health-care article preparation method who contains Chinese medical extract according to claim 1; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
5. a kind of matsutake health-care article preparation method who contains Chinese medical extract according to claim 4 is characterized in that: in said step D, the mutual mixed pH value of matsutake fermented liquid and tricholoma matsutake mycelium extract is 6～7, and the refractive power pol is 2～6%.
6. matsutake health-care article that contain Chinese medical extract, it is characterized in that: said matsutake health-care article are to be prepared from following method:
A, the matsutake bacterium is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated the acquisition strain liquid 72～96 hours with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the tricholoma matsutake mycelium and the matsutake fermented liquid of fermentation;
C, the tricholoma matsutake mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the tricholoma matsutake mycelium extract;
D, the tricholoma matsutake mycelium extract that obtains among matsutake fermented liquid that obtains among the step B and the step C mixed each other after, dilute or concentrate, obtain matsutake health-care article raw material;
In E, the matsutake health-care article raw material that in said step D, obtains, add Chinese medical extract, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
7. a kind of matsutake health-care article that contain Chinese medical extract according to claim 6; It is characterized in that: in said step e, add in the following Chinese medical extract account for matsutake health-care article parts by weight of raw materials one or more: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.
8. a kind of matsutake health-care article that contain Chinese medical extract according to claim 6; It is characterized in that: in said step D; Water or mycelium extract perhaps utilize 2～5 times of said mixed liquor dilutions the reverse osmosis membrane method for concentration or utilize the vacuum method for concentration that said mixed liquor is carried out concentration.
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Cited By (4)
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| CN103550261A (en) * | 2013-11-01 | 2014-02-05 | 王颖 | Method for extracting matsutake essences |
| CN106085792A (en) * | 2016-06-03 | 2016-11-09 | 西藏大学农牧学院 | A kind of preparation method of tricholoma matsutake mycelium fermented type vinegar beverage |
| CN107319318A (en) * | 2017-05-22 | 2017-11-07 | 芜湖市三山区绿色食品产业协会 | The preparation method of radix polygonati officinalis dried mushroom health-care cooked rice crust |
| CN116019844A (en) * | 2022-11-21 | 2023-04-28 | 天津科技大学 | Application of tricholoma matsutake extract in preparation of medicines, health products or foods for preventing or treating Alzheimer disease |
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| CN103550261A (en) * | 2013-11-01 | 2014-02-05 | 王颖 | Method for extracting matsutake essences |
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| CN107319318A (en) * | 2017-05-22 | 2017-11-07 | 芜湖市三山区绿色食品产业协会 | The preparation method of radix polygonati officinalis dried mushroom health-care cooked rice crust |
| CN116019844A (en) * | 2022-11-21 | 2023-04-28 | 天津科技大学 | Application of tricholoma matsutake extract in preparation of medicines, health products or foods for preventing or treating Alzheimer disease |
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