CN102771778A - Grifola frondosa health product and preparation method thereof - Google Patents
Grifola frondosa health product and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a Grifola frondosa health product and a preparation method thereof. The preparation method includes the steps of A, inoculating Grifola frondosa to culture solution, and performing shake culture on a shaker to obtain fungus solution; B, inoculating the fungus solution obtained in the step A to liquid medium containing barely malt and soybean; and performing aerobic or anaerobic culture to obtain fermented Grifola frondosa mycelia and Grifola frondosa fermentation broth; C, subjecting the Grifola frondosa mycelia obtained in the step B to cell tissue breakage, and extracting with water to obtain Grifola frondosa mycelia extract; and D, mixing the Grifola frondosa fermentation broth obtained in the step B and the Grifola frondosa mycelia extract obtained in the step C, and diluting or concentrating obtained liquor to obtain the Grifola frondosa health product. By the industrial submerged fermentation technology, the Grifola frondosa culture can be propagated industrially and massively in the liquid medium. Therefore, the process is simple, and the cost is low.
Description
Technical field
The present invention relates to a kind of health products and preparation method thereof, particularly relate to a kind of grifola frondosus health product that passes through the liquid deep layer fermenting preparation and preparation method thereof.
Background technology
Grifola frondosus formal name used at school: [Grifola frondosa; Polyporus frondosus (Dicks.) Fr. [Boletus frondosus Dicks.]] (have another name called dance fine and soft) be food, medicine dual-purpose gill fungus bacterium.The different name of grifola frondosus is a lot, and taxonomy belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Polyporaceae, tree Pseudomonas.Be a kind of rare food, the dual-purpose bacterium of medicine, Chang Yesheng has good health-care effect and very high medical value between the autumn in summer around chestnut.As a kind of high-class healthy food, be in fashion markets such as Japan, Singapore in recent years.
Because the employing of China's authoritative monograph " fungi of China " early, grifola frondosus just becomes more common Chinese title.Grifola frondosus has pine mushroom appearance fragrance, and meat is tender, and flavor is like shredded chicken, and is crisp like yulan, is extremely precious high-grade edible gill fungus bacterium.
At present, correlative study both domestic and external mainly concentrates in the extraction and pharmacology and nutritive effect of grifola frondosus function factor.But the artificial cultivation field at Grifola Frondosa sporophore but receives technology and environmental limit, is difficult to carry out suitability for industrialized production and civil nature cultivation use widely, and simultaneously, this technical field also is difficult to commercialization and uses.Before 10 years, the year recoverable amount of occurring in nature Grifola Frondosa sporophore has 10,000 tons approximately, 5 kilotons but present recoverable amount is but only had an appointment; And; As stated, Grifola Frondosa sporophore is difficult to a large amount of manual work production again owing to technical reason; Therefore, people have produced significant contradiction to the shortage of craving for the Grifola Frondosa sporophore recoverable amount of grifola frondosus health-care efficacy and therapeutic efficiency.
Summary of the invention
The objective of the invention is to, overcome the defective of above-mentioned prior art and a kind of suitability for industrialized production of can carrying out on a large scale is provided, and can produce the preparation method of the grifola frondosus health product of the higher Grifola Frondosa sporophore of effectiveness with lower cost.
For realizing above-mentioned purpose, the present invention takes following design:
A kind of preparation method of grifola frondosus health product; Said preparation method comprises the steps: A, Grifolas frondosa germ is inserted in the nutrient solution; And under 18～37 ℃ temperature, on shaking table, shake cultivation 48～144 hours with 100～300 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation or anaerobism were cultivated 48～124 hours under 18～37 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the maitake mushroom mycelia extract; D, the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is diluted or concentrates, thereby obtain health drink or health products or health products raw material respectively.
Preferably, in said step D,, thereby obtain the grifola frondosus health drink with 2～5 times of the mixed liquor of gained dilutions.
Preferably, in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.
Preferably, in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.
Preferably, said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 3.0～5.5, and the refractive power pol reaches 2.8～5.8%, and the mycelia yield dry weight reaches 0.35～1.05%.
Another object of the present invention is for providing a kind of grifola frondosus health product; Described grifola frondosus health product is to be prepared from following method: A, Grifolas frondosa germ is inserted in the nutrient solution; And under 18～37 ℃ temperature, on shaking table, shake cultivation 48～144 hours with 100～300 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation or anaerobism were cultivated 48～124 hours under 18～37 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the maitake mushroom mycelia extract; D, the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is diluted or concentrates, thereby obtain health drink or health products or health products raw material respectively.
Preferably, in said step D,, thereby obtain the grifola frondosus health drink with 2～5 times of the mixed liquor of gained dilutions.
Preferably, in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.
Preferably, in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.
Advantage of the present invention is: the present invention adopts that the Grifolas frondosa germ in bacterial classification original producton location separates, purifying and cultivation; Adopt industrialization liquid deep layer fermenting technology, make the grifola frondosus bacterial classification to carry out a large amount of breedings in the liquid medium within, and; All contain in the metabolite of gained and the mycelium and the identical active ingredient of former Grifolas frondosa germ effectiveness; In addition, the present invention also makes the fructification of Grifolas frondosa germ to produce and to cultivate in industrialized condition, and technology is simple, cost is controlled; Thereby can reduce production costs significantly, make ordinary consumer also can use famous and precious health product.
The specific embodiment
Embodiment 1:
Following is the preparation method's of a kind of grifola frondosus health product of the present invention preferred embodiment, and preparation method of the present invention comprises following key step:
A, at first; Grifolas frondosa germ is inserted in the nutrient solution; Nutrient solution of the present invention can be the various nutrient solutions that are suitable for the Grifolas frondosa germ Liquid Culture of the prior art, and under 18 ℃ temperature, on shaking table, shakes cultivation 48 hours with 300 times/minute concussion frequency, obtains strain liquid; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 8% fructus hordei germinatus, weight portion 6% soybean, and inoculum concentration is 5%, and ventilation was cultivated 48 hours under 37 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, again the maitake mushroom mycelia that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.8:5, extraction temperature is 95 ℃, extraction time is 2 hours, thereby obtains the maitake mushroom mycelia extract; Further, polyporus frondosus fermenting liquor and wet thallus that the present invention mainly utilizes industrialization liquid deep layer fermenting technology to obtain prepare and production grifola frondosus health product or health drink; D, last; After the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other; The mixed liquor of gained is diluted or concentrates; Thereby obtain health drink or health products or health products raw material respectively, grifola frondosus health product promptly of the present invention comprises described health drink, health products and health products raw material.Therefore,, promptly can produce popular straight drink product, comprise health products and beverage through preparation method of the present invention; Also can produce the health products raw material, and pass through to add other for example modes of auxiliary material such as Chinese medical extract or sweetener, and further obtain other health products.And preparation method of the present invention can directly apply to suitability for industrialized production, and cost is low, and the products obtained therefrom trophic function is high, helps consumer and public daily health carings.
More specifically, preparation method of the present invention can directly carry out industrial and large-scale and produces continuously.After the batch process, can produce the Grifolas frondosa germ zymotic fluid more than 10 tons every day, and produce 20 tons～final products more than 50 tons every day through preparation method of the present invention.
As stated, a kind of preparation product of present embodiment is a health drink, promptly in said step D, with 2～5 times of the mixed liquor dilutions of gained, thereby obtains the grifola frondosus health drink.Usually adopt water that said mixed liquor is diluted, can certainly adopt other existing liquid of taking that said mixing is diluted, thereby obtain the grifola frondosus health drink.Health drink of the present invention is suitable for regular the drinking of consumer.
More preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 3.0～5.5, and the refractive power pol reaches 2.8～5.8%, and the mycelia yield dry weight reaches 0.35～1.05% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) are preferably and reach relevant parameters separately simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed natural pH value of Grifolas frondosa germ zymotic fluid and maitake mushroom mycelia extract is 6～7, and the refractive power pol is 2～6%.
The content of functional component of the inventive method products therefrom and quality are all highly stable, and are higher than modern pharmacology and research and analyse the numerical value scope of determination to natural Cordyceps militaris component content.Preparation method's of the present invention industrialization control ability is strong, and its cost is far below natural material.
Embodiment 2:
Preparation method's of the present invention another kind of preferred embodiment will be provided as follows.The preparation method of present embodiment comprises: A, at first, select food, medicinal bacterium grifola frondosus for use, and Grifolas frondosa germ is inserted in the nutrient solution, and under 37 ℃ temperature, on shaking table, shake and cultivated 144 hours with 100 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 12% fructus hordei germinatus, weight portion 3% soybean; Inoculum concentration is 10%; And 124 hours (also can carry out anaerobism as required cultivates) cultivated in ventilation under 18 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 1:2, extraction temperature is 90 ℃, extraction time is 3 hours, thereby obtains the maitake mushroom mycelia extract; D, last; After the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other; The mixed liquor of gained is diluted or concentrates; Thereby obtain health drink or health products or health products raw material respectively, grifola frondosus health product promptly of the present invention comprises described health drink, health products and health products raw material.
A kind of preparation product of present embodiment is the grifola frondosus health products, promptly in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.Maitake mushroom mycelia content in this enriched product is higher, therefore, has the effectiveness that keeps healthy preferably.
Preferably; The mixed liquor method for concentration that in preparation method's of the present invention step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably; In the present embodiment, adopt the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained grifola frondosus health product, the content of its functional components and Grifola Frondosa sporophore are close or be higher than Grifola Frondosa sporophore, and its effectiveness is with directly to take grifola frondosus suitable.
Embodiment 3:
Another preferred embodiment of preparation method of the present invention will be provided as follows.The preparation method of present embodiment comprises the steps: A, Grifolas frondosa germ is inserted in the nutrient solution, and under 28 ℃ temperature, on shaking table, shakes cultivation 80 hours with 200 times/minute concussion frequency, obtains strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 10% fructus hordei germinatus, weight portion 5% soybean; Inoculum concentration is 7%; And ventilation was cultivated 70 hours under 25 ℃ temperature, through biofermentation, obtained the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.9:4, extraction temperature is 93 ℃, extraction time is 2.5 hours, thereby obtains the maitake mushroom mycelia extract; D, last; After the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other; The mixed liquor of gained is diluted or concentrates; Thereby obtain health drink or health products or health products raw material respectively, grifola frondosus health product promptly of the present invention comprises described health drink, health products and health products raw material.
A kind of preparation product of present embodiment is grifola frondosus health products raw materials, promptly in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.In this step; Through in the grifola frondosus health products raw material of preparing, adding Chinese medical extract; Thereby can obtain to have the health product of comprehensive health-care function; Make the health care effectiveness of maitake mushroom mycelia and the pharmacological property between the Chinese medical extract to mutually promote, therefore, can carry out comprehensive conditioning and health care consumer person healthy.Preferably, Chinese medicine of the present invention comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
Embodiment 4:
Present embodiment provides a kind of grifola frondosus health product through the liquid deep layer fermenting preparation; Said grifola frondosus health product is to be prepared from following method: A, will select for use food, medicinal Grifolas frondosa germ to insert in the nutrient solution; And under 18～28 ℃ temperature (present embodiment is preferably 20 ℃), on shaking table, shake 72～96 hours (present embodiment is preferably 82 hours) of cultivation with the concussion frequency of 200～300 times/minute (present embodiment is preferably 260 times/minute), obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% (present embodiment is preferably 11%) fructus hordei germinatus, weight portion 3～6% (present embodiment is preferably 5%) soybean; Inoculum concentration is 5～10% (present embodiment is preferably 8%); And ventilation is cultivated 48～72 hours (present embodiment is preferably 56 hours under the temperature of 23～27 ℃ (present embodiment is preferably 26 ℃); Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation; C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after; Water extracts; The lixiviate ratio is 0.8～1:2～5 (present embodiment is preferably 1:3); Extraction temperature is 90～95 ℃ (present embodiment is preferably 92 ℃), and extraction time is 2.5 hours, thereby obtains the maitake mushroom mycelia extract; D, last; After the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other; The mixed liquor of gained is diluted or concentrates; Thereby obtain health drink or health products or health products raw material respectively, grifola frondosus health product promptly of the present invention comprises described health drink, health products and health products raw material.
As stated, a kind of preparation product of the present invention is a health drink, promptly in said step D, with 2～5 times of the mixed liquor dilutions of gained, thereby obtains the grifola frondosus health drink.Usually adopt water that said mixed liquor is diluted, can certainly adopt other existing liquid of taking that said mixing is diluted, thereby obtain the grifola frondosus health drink.Health drink of the present invention is suitable for regular the drinking of consumer.
Another kind of preparation product of the present invention is the grifola frondosus health products, promptly in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.Maitake mushroom mycelia content in this enriched product is higher, therefore, has the effectiveness that keeps healthy preferably.
Another preparation product of the present invention is grifola frondosus health products raw materials, promptly in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.In this step; Through in the grifola frondosus health products raw material of preparing, adding Chinese medical extract; Thereby can obtain to have the health product of comprehensive health-care function; Make the health care effectiveness of maitake mushroom mycelia and the pharmacological property between the Chinese medical extract to mutually promote, therefore, can carry out comprehensive conditioning and health care consumer person healthy.Preferably, Chinese medicine of the present invention comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
Particularly, add in the following Chinese medical extract account for grifola frondosus health products parts by weight of raw materials one or more in the present invention: the Rhizoma Polygonati Odorati extract of the Bulbus Lilii extract of the licorice of the yam extract of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the Fructus lycii P.E of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the honey of 0.3～0.8% weight portion, 0.3～0.8% weight portion, the tuckahoe extracts of 0.3～0.8% weight portion, the kudzu root extract of 0.3～0.8% weight portion.Be understood that to those skilled in the art, in above-mentioned weight portion scope, can choose the corresponding Chinese medical extract of arbitrary proportion, thereby process health product with comprehensive health-care function.
Wherein, the method for concentration that in step D, is adopted can be reverse osmosis membrane method for concentration or vacuum method for concentration, preferably, in the present embodiment, adopts the reverse osmosis membrane method for concentration that said mixed liquor is carried out concentration.And further, when present embodiment adopted the reverse osmosis membrane method for concentration, the film device operating pressure position of reverse osmosis membrane method for concentration was set in 40kg/cm
2Below, and under room temperature, operate, thus obtained grifola frondosus health product, its effectiveness is with directly to take grifola frondosus suitable.
Preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 3.0～5.5, and the refractive power pol reaches 2.8～5.8%, and the mycelia yield dry weight reaches 0.35～1.05% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) reach corresponding separately index simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed pH value of Grifolas frondosa germ zymotic fluid and maitake mushroom mycelia extract is 6～7, and the refractive power pol is 2～6%.
Other embodiment of a kind of grifola frondosus health product through liquid deep layer fermenting preparation of the present invention are identical or close with the foregoing description 1,2 and 3, repeat no more at this.To those skilled in the art, be not difficult from the foregoing description 1,2 and 3, to obtain relevant teachings, and in order to preparation or production grifola frondosus health product.
Obviously, those of ordinary skill in the art can use a kind of grifola frondosus health product that passes through the liquid deep layer fermenting preparation of the present invention and preparation method thereof, forms various types of health products and preparation method thereof.
The foregoing description only supplies to explain the present invention's usefulness; And be not to be limitation of the present invention; The those of ordinary skill in relevant technologies field without departing from the present invention, can also be made various variations and modification; Therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited each claim.
Claims (10)
1. the preparation method of a grifola frondosus health product, it is characterized in that: said preparation method comprises the steps:
A, Grifolas frondosa germ is inserted in the nutrient solution, and under 18～37 ℃ temperature, on shaking table, shake and cultivated 48～144 hours, obtain strain liquid with 100～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation or anaerobism were cultivated 48～124 hours under 18～37 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation;
C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the maitake mushroom mycelia extract;
D, the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is diluted or concentrates, thereby obtain health drink or health products or health products raw material respectively.
2. the preparation method of a kind of grifola frondosus health product according to claim 1 is characterized in that: in said step D, with 2～5 times of the mixed liquor of gained dilutions, thereby obtain the grifola frondosus health drink.
3. the preparation method of a kind of grifola frondosus health product according to claim 1 is characterized in that: in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.
4. the preparation method of a kind of grifola frondosus health product according to claim 1 is characterized in that: in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.
5. the preparation method of a kind of grifola frondosus health product according to claim 4 is characterized in that: said Chinese medicine comprises Chinese yam or Radix Glycyrrhizae or the fruit of Chinese wolfberry or lily or honey or radix polygonati officinalis or Poria cocos or the root of kudzu vine or several kinds mixture wherein.
6. according to the preparation method of any described a kind of grifola frondosus health product of claim 1～5; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 3.0～5.5, and the refractive power pol reaches 2.8～5.8%, and the mycelia yield dry weight reaches 0.35～1.05%.
7. grifola frondosus health product, it is characterized in that: said grifola frondosus health product is to be prepared from following method:
A, Grifolas frondosa germ is inserted in the nutrient solution, and under 18～37 ℃ temperature, on shaking table, shake and cultivated 48～144 hours, obtain strain liquid with 100～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation or anaerobism were cultivated 48～124 hours under 18～37 ℃ temperature; Through biofermentation, obtain the maitake mushroom mycelia and the Grifolas frondosa germ zymotic fluid of fermentation;
C, the maitake mushroom mycelia that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the maitake mushroom mycelia extract;
D, the maitake mushroom mycelia extract that obtains among Grifolas frondosa germ zymotic fluid that obtains among the step B and the step C mixed each other after, the mixed liquor of gained is diluted or concentrates, thereby obtain health drink or health products or health products raw material respectively.
8. a kind of grifola frondosus health product according to claim 7 is characterized in that: in said step D, with 2～5 times of the mixed liquor of gained dilutions, thereby obtain the grifola frondosus health drink.
9. a kind of grifola frondosus health product according to claim 7 is characterized in that: in said step D, the mixed liquor of gained is concentrated 4～7 times, and make its refractive power pol reach 10～12%, thereby obtain the grifola frondosus health products.
10. a kind of grifola frondosus health product according to claim 7 is characterized in that: in said step D, with the mixed liquor dilution or the concentrated grifola frondosus health products raw material that obtains of gained; And be included in the said grifola frondosus health products raw material step that adds Chinese medical extract.
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| CN103893465A (en) * | 2012-12-31 | 2014-07-02 | 赛珂睿德生物医药科技(上海)有限公司 | Chinese herbal compound extract as well as preparation method and application thereof |
| CN105995291A (en) * | 2016-06-13 | 2016-10-12 | 于汇 | Edible and medicinal fungus healthcare and beautifying functional beverage for females |
| CN111484940A (en) * | 2020-04-22 | 2020-08-04 | 合肥迈可罗生物工程有限公司 | Preparation method and application of fungus product |
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Application publication date: 20121114 |