CN103893465A - Chinese herbal compound extract as well as preparation method and application thereof - Google Patents
Chinese herbal compound extract as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103893465A CN103893465A CN201210589814.4A CN201210589814A CN103893465A CN 103893465 A CN103893465 A CN 103893465A CN 201210589814 A CN201210589814 A CN 201210589814A CN 103893465 A CN103893465 A CN 103893465A
- Authority
- CN
- China
- Prior art keywords
- extract
- chinese medicinal
- compound extract
- enhancing immunity
- medicinal compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a Chinese herbal compound extract; the extract is characterized by comprising the following traditional Chinese medicines in percentage by weight: 4-60% of grifola frondosa, 4-60% of mushrooms, 4-60% of platycodon grandiflorum, 4-60% of white lablab beans, 4-60% of yam, 4-60% of Chinese wolfberry fruit and 4-60% of hawthorn; the preparation method comprises the following steps: carefully selecting such raw materials as grifola frondosa, mushrooms, platycodon grandiflorum, white lablab beans, yam, Chinese wolfberry fruit and hawthorn, and removing impurities, cleaning and grinding, completely soaking the raw materials in proper amount of water, decocting and concentrating, precipitating through proper amount of ethanol, drying precipitate and implementing other steps to obtain dry powder; adding pharmaceutically acceptable accessories to the dry powder to prepare such preparation as powder, granules, capsules, tablets, suspensions and oral liquid. The extract disclosed by the invention is scientific in compatibility, reasonable in preparation process and capable of effectively preserving effective ingredients in various raw materials; the Chinese herbal compound extract is free from toxic and side effect, good in taste and easy to be accepted by human body, and has a function of enhancing immunity.
Description
Technical field
The present invention relates to a kind of Chinese medicinal compound extract, more particularly, relate to a kind of from Grifola frondosa (Griflola frondosa), Lentinus Edodes (Lentinula edodes (Berk.) Pegler.), Radix Platycodonis (Platycodon grandiflorum (Jacq.) A.DC.), Semen Lablab Album (Dolichos lablab L.), Rhizoma Dioscoreae (Dioscorea opposita Thunb.), Fructus Lycii (Lycium barbarum L.), Fructus Crataegi (Crataegus pinnatifida Bge.; Crataegus pinnatifida Bge.var.major N.E.Br.) in extract extract of obtaining and preparation method thereof and the purposes of this extract in the medicine for the preparation of enhancing immunity function.
Background technology
Modern study proves, the state that perfects of the aging of body and immune defense function has substantial connection.The appropriate regulation of immune system on cell and molecular level, can slow down aging.Human body body's immunity declines as a kind of stealthy killer who endangers modern's health, has had a strong impact on people's work and life, has increased sub-health population quantity.
Grifola frondosa, Lentinus Edodes, Radix Platycodonis, Rhizoma Dioscoreae, Fructus Lycii, Semen Lablab Album has respectively good enhancing immunity function
Grifola frondosa Griflola frondosa is a kind of rare fungus of the food medicine dual-purpose that is grown in subtropical zone to temperate zone, is widely used as a kind of high-class healthy food in recent years.Its polysaccharide is active cell immunologic function greatly, improves immunity of organisms, and research report grifolan all has activation to panimmunity cell.
Lentinus Edodes Lentinula edodes (Berk.) Pegler.Its polysaccharide has immunoregulatory effect, is typical t cell activation agent, can promote the generation of interleukin, is considered to a kind of specifc immunity reinforcing agent.
Radix Platycodonis is the dry root of Campanulaceae Platycodon grandiflorum (Jacq.) A.DC..Its platycodon root polysaccharide has immunoregulation effect.Can significantly improve propagation and the transfer ability of macrophage, increase the generation of cytokine profiles in macrophage, be a kind of potential macrophage function reinforcing agent.
Rhizoma Dioscoreae is the dry rhizome of Dioscoreaceae plant Dioscorea opposita Thunb..Its polysaccharide has the body's immunity of adjusting curative effect, has nonspecific immunity regulatory function and improves specific cellular immunity and humoral immune function, therefore comprehensive immunologic function of improving body is had great importance.
Fructus Lycii is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..Lycium barbarum polysaccharide has immunomodulating, activity of fighting against senium; In cellular immunization and humoral immunization adjusting, play an important role.
Semen Lablab Album is the dry mature seed of leguminous plant Dolichos lablab L..Semen Lablab Album polysaccharide can significantly improve phagocytic percentage and the phagocytic index of normal mouse peritoneal macrophage, promotes hemolysin to form, and the humoral immune function of mice is had to certain potentiation
Summary of the invention
The present invention utilizes simple and effective extraction process and method, take seven kinds of integration of edible and medicinal herbs article as raw material has obtained a kind of Chinese medicinal compound extract.Pharmacological evaluation shows that this Chinese medicinal compound extract has mediator's body immunity function and corresponding enhancing immunity functional activity.Therefore this Chinese medicinal compound extract is expected to exploitation becomes a kind of as enhancing immunity functional health product or medicine or assist a ruler in governing a country medicine and for clinical.
Therefore, one object of the present invention is to provide a kind of active chemical and clearly compound preparation of prescription weight ratio.
Another object of the present invention is to provide a kind of Chinese medicinal compound extract preparation method.
A further object of the present invention is to provide a kind of Chinese medicinal compound extract for enhancing immunity function, its compositions that comprises the Chinese medicine extract in 100-1000mg/kg/d effective dosage ranges is as effective ingredient, and preferably, it can further contain medically acceptable carrier and adjuvant.
Another object of the present invention is to provide the purposes of Chinese medicine extract in the medicine for the preparation of enhancing immunity function.
In the present invention, described Chinese medicinal compound extract consists of Grifola frondosa, Lentinus Edodes, Radix Platycodonis, Rhizoma Dioscoreae, Fructus Lycii, Fructus Crataegi, Semen Lablab Album.
In the present invention, described Chinese medicinal compound extract is filbert powder, sweet in the mouth, feeble QI.
According to another object of the present invention, the invention provides the method for this Chinese medicinal compound extract of preparation.
(1) from this Chinese medicinal compound extract, extract and separate and purification, comprise the steps:
(1) get dry Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album, the heating that adds water decocts.The 1kg raw material 5-7L heated and boiled that at every turn adds water, extraction time is 1-3 hour, extracts altogether 3 times; Concentrated extracting solution, is cooled to room temperature; Slowly add 3.75 times of volume 95% edible ethanols, making its final concentration is 75% (w/v); Room temperature leaves standstill 3-5 hour;
(2) the upper strata ethanol that inclines, precipitation is filtered dry ethanol, then with a small amount of ethanol rinse precipitation 1-3 time, is filtered dry ethanol;
(3) precipitation, with 55 ℃ of oven dry, is ground into powder through cracker for medicine; Gained powder is the Chinese medicinal compound extract of tool enhancing immunity function.
Wherein, the alcoholic solution described in step (1) is 95% edible ethanol.
The condition of the boiling water extraction described in step (1) is: add about 5-7L distilled water, extraction time is 1-3 hour at every turn, extracts 3～5 times, preferably extracts 3 times.
Ethanol deposition condition described in step (1) is: slowly add 3.75 times of volume 95% edible ethanols, making its final concentration is 75% (w/v).
Drying means described in step (3) is: 55 ℃ of normal pressures are dried.
In a preferred embodiment of the invention, preparing Chinese medicinal compound extract comprises the steps:
(1) get dry Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album, the heating that adds water decocts.The 1kg raw material 5-7L heated and boiled that at every turn adds water, extraction time is 2 hours, extracts altogether 3 times; Concentrated extracting solution, is cooled to room temperature; Slowly add 3.75 times of volume 95% edible ethanols, making its final concentration is 75% (w/v); Room temperature leaves standstill 3 hours; The upper strata ethanol that inclines, precipitation is filtered dry ethanol, then with a small amount of ethanol rinse precipitation secondary, is filtered dry ethanol; Precipitation, with 55 ℃ of oven dry, is ground into powder through cracker for medicine; Gained powder is the Chinese medicinal compound extract (yield: 14%) of tool enhancing immunity function.
According to a further object of the present invention, the invention provides a kind of Chinese medicinal compound extract for enhancing immunity function, its compositions that comprises the Chinese medicine extract in 100-1000mg/kg/d effective dosage ranges is as effective ingredient, and preferably, it can further contain medically acceptable carrier and adjuvant.
Extract of the present invention can oral administration administration.When oral administration administration, can directly or first make such extract mix with conventional medicinal adjuvant (as excipient, disintegrating agent, adhesive, lubricant, antioxidant, coating materials, coloring agent, aromatic, surfactant etc.), be made into the forms such as granule, capsule, tablet.While preparing above-mentioned preparation, can use conventional preparation technique.
According to another object of the present invention, the invention provides the purposes of said composition in the medicine for the preparation of enhancing immunity function.The present invention utilizes simple and effective extraction process and method, take Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album as raw material.Pharmacological evaluation shows that this extract has enhancing immunity function and corresponding enhancing immunity activity.Therefore this extract is expected to exploitation becomes a kind of health promoting product as enhancing immunity function or medicine or assists a ruler in governing a country medicine for clinical.
Accompanying drawing explanation
Fig. 1 is according to the separation and purification flow chart of the extract of the enhancing immunity function of an embodiment of the invention.
The specific embodiment
Below in conjunction with embodiment, the present invention is further elaborated, below embodiment the present invention is only described by way of example.Clearly, those of ordinary skills can, in scope of the present invention and essence, carry out various accommodations and modification to the present invention.Need to be appreciated that, this invention is intended to be encompassed in the flexible and modification that appended claims comprises.
Instrument:
The CR-22G of Hitachi type high speed centrifuge (Japanese Hitachi company), electric heating constant-temperature blowing drying box (the upper grand experimental facilities of Nereid company limited, EYELA-N1100 type Rotary Evaporators.
Medicine and reagent:
Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album entity are purchased from Hu Qingyu Pharmaceutical Workshop, Shanghai pharmacy.
Grifola frondosa, Lentinus Edodes entity are purchased from Qingyuan County, Zhejiang wholesale market of agricultural products.
Edible ethanol derives from Shanghai nine through commerce and trade company limited.
The preparation of embodiment 1 Chinese medicinal compound extract:
Get dry Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album (respectively purchased from Hu Qingyu Pharmaceutical Workshop, Shanghai pharmacy; Qingyuan County, Zhejiang wholesale market of agricultural products) heating decoction adds water.The 1kg raw material 5-7L heated and boiled that at every turn adds water, extraction time is 1-3 hour, extracts altogether 3 times; Concentrated extracting solution, is cooled to room temperature; Slowly add 3.75 times of volume 95% edible ethanols, making its final concentration is 75% (w/v); Room temperature leaves standstill 3-5 hour; The upper strata ethanol that inclines, precipitation is filtered dry ethanol, then with a small amount of ethanol rinse precipitation 1-3 time, is filtered dry ethanol; Precipitation, with 55 ℃ of oven dry, is ground into powder through cracker for medicine; Gained powder is the Chinese medicinal compound extract of tool enhancing immunity function, is filbert pressed powder.
EXPERIMENTAL EXAMPLE
Experimental example 1 pharmacological evaluation:
The mouse spleen lymphocyte transformation experiment of 1.ConA induction
1.1 experiment material
(a) raw material: filbert powder (SF1), (Shanghai) Co., Ltd. provides by the farsighted moral biological medicine science and technology of match Mactra sulcatria Deshayes.
(b) experiment mice: female Balb/C mice, 18-22g, purchased from Shanghai Si Laike Experimental Animal Center.Feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature is controlled at 20-25 ℃, humidity 55-65%, and illumination 10-12 hour/day, mouse cage is changed 2-3 time for one week.
(c) instrument and material; RPMI1640 cell culture fluid, hyclone, Young Men's Association's mycin, concanavalin A, Con A (ConA, Sigma), hydrochloric acid, isopropyl alcohol, MTT, HBSS liquid, PBS buffer (pH 7.2-7.4) 200 eye mesh screens, 24 well culture plates, 96 well culture plates, operating theater instruments, CO2 gas incubator, microplate reader, superclean bench, high-pressure sterilizing pot, sterile filters.
1.2 experimental technique
(a) testing each component is 125,250,500 and tetra-dosage groups of 1000ug/ml and a negative control group, continuous action 72 hours.
(b) divide two holes to add in 24 well culture plates every a splenocyte suspension, every hole 1ml, a hole adds 75ul Con A liquid (final concentration 7.5ug/ml), and 5%CO in contrast, is put in another hole
2, in 37 ℃ of incubators, cultivate 72 hours, to cultivate and finish first 4 hours, every hole sucks supernatant 0.7ml gently, adds 0.7ml not containing the RPMI RPMI-1640 of serum, adds MTT (5mg/ml) 50ul/ hole simultaneously, continues to cultivate 4 hours.After cultivation finishes, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixes, and purple crystal is dissolved completely.Then divide and install in 96 well culture plates, three parallel holes are done in each hole, by microplate reader, measure optical density value with 570nm wavelength.
Table 1.SF1 inducing mouse splenocyte propagation OD, T/C (%) value
When experiment finishes, 125,250,500 and each group of 1000ug/ml, through with splenocyte co-cultivation after 72 hours, optical density value is significantly higher than matched group optical density value, can judge this experimental result positive.
Experimental example 2 pharmacological evaluation:
2. delayed allergy (dinitrofluorobenzene inducing mouse ear swelling method)
2.1 experiment material
(a) raw material: filbert powder (SF1), (Shanghai) Co., Ltd. provides by the farsighted moral biological medicine science and technology of match Mactra sulcatria Deshayes
(b) experiment mice: female Balb/C mice, 18-22g, purchased from Shanghai Si Laike Experimental Animal Center.Feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature is controlled at 20-25 ℃, humidity 55-65%, and illumination 10-12h/d, mouse cage is changed 2-3 time for one week.
(c) instrument and material: DNFB, acetone, Oleum Sesami, card punch.
2.2 experimental technique
(a) testing each component is 100,300 and tri-dosage groups of 1000mg/kg and a negative control group, continuously gavage 7 days.
(b) every Mus skin of abdomen razor blade unhairing, avoids scratch skin when unhairing, and scope is at 3cm*3cm, and with DNFB solution, 50ul evenly smears sensitization.7 days use afterwards, is evenly applied in mouse right ear (two sides) with DNFB solution 10ul and attacks.Attack cervical vertebra dislocation in latter 24 hours and put to death mice, cut left and right auricular concha.Take off the auricle of diameter 8mm with card punch, weigh.
Table 2SF1. treatment dinitrofluorobenzene inducing mouse ear swelling body weight, ear thickness and spleen ratio
When experiment finishes, 300mg/kg group, ear thickness is significantly higher than matched group, and spleen ratio is also significantly higher than matched group, can judge this experimental result positive.
Experimental example 3 pharmacological evaluation:
3 antibody-producting cells detect (Jerne improves slide method)
3.1 experiment material
(a) raw material: filbert powder (SF1), (Shanghai) Co., Ltd. provides by the farsighted moral biological medicine science and technology of match Mactra sulcatria Deshayes
(b) experiment mice: female Balb/C mice, 18-22g, purchased from Shanghai Si Laike Experimental Animal Center.Feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature is controlled at 20-25 ℃, humidity 55-65%, and illumination 10-12 hour/day, mouse cage is changed 2-3 time for one week.
(c) CO2 gas incubator, water bath with thermostatic control, centrifuge, operating theater instruments, slide frame, 200 eye mesh screens, SRBC (Zhengzhou hundred base biologies), complement (guinea pig serum) (Zhengzhou hundred base biologies), Hank ' s liquid (Hyclone), agarose.
3.2 experimental technique
(a) testing each component is 100,300 and tri-dosage groups of 1000mg/kg and a blank group, continuously gavage 4 weeks
(b) by after top layer culture medium (1g agarose adds distilled water to 100ml) heating for dissolving, put 45-50 ℃ of water bath heat preservation, mix with Hank ' the s liquid of equivalent volumes pH 7.2-7.4,2 times of concentration, be sub-packed in small test tube, every pipe 0.5ml, then add 50 μ L 10%SRBC and 20 μ L splenocyte suspensions (5 × 10 in pipe
6individual/ml), mix rapidly, be poured on the slide of brushing agarose thin layer, do parallel plate, after agar solidifies, slide level is buckled and is placed on horse, put into CO2 gas incubator and hatch 1-1.5 hour, then use the complement (1: 8) of normal saline dilution to join slide frame groove, after continuing to be incubated overnight, counting hemolysis plaque number.
Table 3.SF1 antagonist cellulation detects
When experiment finishes, SF11000mg/kg group plaque number is significantly higher than matched group, can judge this experimental result positive.
Experimental example 4 pharmacological evaluation:
4. the mensuration of serum hemolysin (Hemagglutination Method)
4.1 experiment material
(a) raw material: filbert powder (SF1), (Shanghai) Co., Ltd. provides by the farsighted moral biological medicine science and technology of match Mactra sulcatria Deshayes
(b) experiment mice: female Balb/C mice, 18-22g, purchased from Shanghai Si Laike Experimental Animal Center.Feed with sterilizing plastics mouse cage, freely drink water, Animal House temperature is controlled at 20-25 ℃, humidity 55-65%, and illumination 10-12 hour/day, mouse cage is changed 2-3 time for one week.
(c) SRBC, normal saline, Microhemagglutination brassboard (Haimen Tian Yan experiment equipment business department), centrifuge
4.2 experimental technique
(a) testing each component is 100,300 and tri-dosage groups of 1000mg/kg and a blank group, continuously gavage 4 weeks.
(b with normal saline by serum doubling dilution, dilution difference serum is placed in respectively in Microhemagglutination brassboard, every hole 100 μ L, add again the SRBC suspension of 100 μ l 0.5% (v/v), mix, pack in moistening square position and add a cover, in 37 ℃ of incubations 3 hours or spend the night, observe hemagglutination degree.
The mensuration of table 4.SF1 to serum hemolysin
When experiment finishes, SF1100,300,1000mg/kg group, antibody horizontal is significantly higher than matched group, can judge this experimental result positive.
Claims (8)
1. there is a Chinese medicinal compound extract for enhancing immunity, it is characterized in that being formed by the raw material of following weight percentage ratio: Grifola frondosa 4-60%, Lentinus Edodes 4-60%, Radix Platycodonis 4-60%, Semen Lablab Album 4-60%, Rhizoma Dioscoreae 4-60%, Fructus Lycii 4-60%, Fructus Crataegi 4-60%.
(1). by a kind of Chinese medicinal compound extract with enhancing immunity function claimed in claim 1, it is characterized in that the extract of being made by the raw material of following weight percentage ratio: Grifola frondosa 16%, Lentinus Edodes 14%, Radix Platycodonis 14%, Semen Lablab Album 14%, Rhizoma Dioscoreae 14%, Fructus Lycii 14%, Fructus Crataegi 14%.
(2). by a kind of Chinese medicinal compound extract with enhancing immunity function claimed in claim 1, it is characterized in that the extract of being made by the raw material of following weight percentage ratio: Grifola frondosa 40%, Lentinus Edodes 10%, Radix Platycodonis 15%, Semen Lablab Album 10%, Rhizoma Dioscoreae 10%, Fructus Lycii 10%, Fructus Crataegi 5%.
(3). by a kind of Chinese medicinal compound extract with enhancing immunity function claimed in claim 1, it is characterized in that the extract of being made by the raw material of following weight percentage ratio: Grifola frondosa 5%, Lentinus Edodes 5%, Radix Platycodonis 30%, Semen Lablab Album 20%, Rhizoma Dioscoreae 5%, Fructus Lycii 5%, Fructus Crataegi 30%.
2. a method of preparing Chinese medicinal compound extract as claimed in claim 1, comprises the steps:
(1) get Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album crude drug, boiling water extraction 3 to 6 times, each 2 hours-3 hours;
(2) filtrate is concentrated into small size, is cooled to room temperature, slowly adds 2-10 times of volume 95% edible ethanol, after 2-8 hour, and centrifugal must precipitation;
(3) precipitation oven drying at low temperature 10-24 hour, pulverizes, and obtains powder; Gained powder is the Chinese medicinal compound extract of tool enhancing immunity function.
3. according to the enhancing immunity Chinese medicinal compound extract preparation described in any one in claim 1 to 2, it is characterized in that described medicament is said dosage form on any pharmaceutics.
4. method according to claim 2, is characterized in that, the condition of the boiling water extraction described in step (1) is: solid-liquid ratio (1: 7-10), extraction time is 2-3 hour, extracts 3～4 times, preferably extracts 3 times.
5. method according to claim 2, is characterized in that, the alcoholic solution described in step (2) is 95% edible ethanol, is preferably 3.75: 1 by ethanol and water extraction liquid volume ratio.
6. method according to claim 2, is characterized in that, comprises the steps:
(1) get dry Grifola frondosa, Lentinus Edodes, Fructus Lycii, Radix Platycodonis, Fructus Crataegi, Rhizoma Dioscoreae, Semen Lablab Album, the heating that adds water decocts.The 1kg raw material 5-7L heated and boiled that at every turn adds water, extraction time is 1-3 hour, extracts altogether 3 times; Concentrated extracting solution, is cooled to room temperature; Slowly add 3.75 times of volume 95% edible ethanols, making its final concentration is 75% (w/v); Room temperature leaves standstill 3-5 hour;
(2) the upper strata ethanol that inclines, precipitation is filtered dry ethanol, then with a small amount of ethanol rinse precipitation 1-3 time, is filtered dry ethanol;
(3) precipitation, with 55 ℃ of oven dry, is ground into powder through cracker for medicine; Gained powder is the Chinese medicinal compound extract of tool enhancing immunity function.
7. above-mentioned dry powder adds after medically acceptable adjuvant, can be made into the preparations such as powder, granule, capsule, tablet, suspensoid, oral liquid.
8. the purposes of Chinese medicinal compound extract claimed in claim 1 in the medicine for the preparation of enhancing immunity function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589814.4A CN103893465A (en) | 2012-12-31 | 2012-12-31 | Chinese herbal compound extract as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589814.4A CN103893465A (en) | 2012-12-31 | 2012-12-31 | Chinese herbal compound extract as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103893465A true CN103893465A (en) | 2014-07-02 |
Family
ID=50985249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210589814.4A Pending CN103893465A (en) | 2012-12-31 | 2012-12-31 | Chinese herbal compound extract as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103893465A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999578A (en) * | 2010-10-29 | 2011-04-06 | 无限极（中国）有限公司 | Health food suiting child dinners and having immunity enhancing function |
| CN102599570A (en) * | 2012-03-13 | 2012-07-25 | 河南工业大学 | Chinese yam, wolfberry and hawthorn compound beverage |
| CN102771778A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Grifola frondosa health product and preparation method thereof |
-
2012
- 2012-12-31 CN CN201210589814.4A patent/CN103893465A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999578A (en) * | 2010-10-29 | 2011-04-06 | 无限极（中国）有限公司 | Health food suiting child dinners and having immunity enhancing function |
| CN102599570A (en) * | 2012-03-13 | 2012-07-25 | 河南工业大学 | Chinese yam, wolfberry and hawthorn compound beverage |
| CN102771778A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Grifola frondosa health product and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 班纪华: "《药用野生蔬菜栽培与开发利用技术》", 30 September 2011, 中国农业出版社 * |
| 郑建仙主编: "《功能性食品学》", 30 June 2009, 中国轻工业出版社 * |
| 马丙祥等: "《实用儿科诊断与治疗》", 30 September 1997, 科学技术文献出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109276576B (en) | Application of ganoderma leucocontextum polysaccharide in preparation of antitumor drugs | |
| CN102417544A (en) | Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof | |
| CN104116755A (en) | Ganoderma lucidum, wolfberry and rhizoma polygonati compound polysaccharide with tumor microenvironment regulation function as well as preparation method and application thereof | |
| CN103182049A (en) | Preparation method of pharmaceutical composition preparation treating apoplexy sequelae | |
| CN102716223B (en) | Chinese medicinal composition for treating upper respiratory tract infection and preparation method thereof | |
| CN100358532C (en) | Total flavone of citrus grandis osbeckvar tomentosa hort peridium, and its extracting method and use | |
| CN107722131B (en) | Total ganoderma lucidum spore powder refined polysaccharide with significant auxiliary antitumor activity and preparation method and application thereof | |
| CN103860602A (en) | Application of phellinus igniarius extract in preparation of medicine for treating liver cancer, cervical cancer and breast cancer | |
| CN107137438B (en) | The purposes of wintersweet platymiscium anti-mycobacterium tuberculosis | |
| CN108813610A (en) | A kind of saussurea involucrata composition and its application for improving immunity | |
| CN105796587B (en) | Caulis bambusae in taenian polysaccharide immunological regulation, it is antitumor in application | |
| CN102071239A (en) | Anti-tumor mung bean polypeptide, preparation method thereof and application thereof to tumors | |
| CN102178701B (en) | Preparation method of polysaccharide composite with antitumor activity | |
| CN101735330A (en) | Preparation method of yew polysaccharide with function of resisting tumors and application thereof | |
| CN104306401A (en) | Preparation and application of sika deer spleen extract having function of increasing white blood cells | |
| CN103830280A (en) | Preparation method of spirulina extract | |
| CN103893465A (en) | Chinese herbal compound extract as well as preparation method and application thereof | |
| CN103550243B (en) | The application of Rhizoma Atractylodis polysaccharide in the medicine improving kuffer cellular immune function or health product | |
| Nan et al. | Effects of Lycium barbarum polysaccharides (LBP) on immune function of mice | |
| CN103520222A (en) | Cordyceps militaris extractive and application thereof in preparation of medicines for treating tumors | |
| CN102357151A (en) | Chinese medicinal composition for delaying senility and preparation process thereof | |
| CN100589812C (en) | Plant worms mycelium extracat fraction and composition for oral intake | |
| CN101711790A (en) | Wild Juglans mandshurica bark water extract used for curing liver cancer | |
| CN101502536B (en) | Cedar total flavone as well as preparation method and medical use | |
| CN102068537B (en) | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140702 |
|
| WD01 | Invention patent application deemed withdrawn after publication |