CN103830280A - Preparation method of spirulina extract - Google Patents
Preparation method of spirulina extract Download PDFInfo
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- CN103830280A CN103830280A CN201410087576.6A CN201410087576A CN103830280A CN 103830280 A CN103830280 A CN 103830280A CN 201410087576 A CN201410087576 A CN 201410087576A CN 103830280 A CN103830280 A CN 103830280A
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Abstract
The invention relates to a preparation method of a spirulina extract, belonging to the technical field of biological medicine. The method comprises the following steps: (a) wall breaking: grinding spirulina to 600-800 meshes to obtain spirulina broken-wall powder; (b) fermentation: fermenting the spirulina broken-wall powder in amillariella mellea-rich fermentation liquid for 6-10 hours; (c) drying: drying with a drying device to obtain dry powder; (d) extraction: extracting the dry powder with water, and extracting a water extract and an extract respectively from the residue after the water extraction by use of supercritical CO2. The method provided by the invention has the beneficial effects that microcystin in the spirulina can be effectively degraded and removed; according to detection, the content of microcystin in the spirulina extract obtained by the method is less than or equal to 90pg/g, which is much lower than the content of microcystin in the existing spirulina products.
Description
Technical field
The invention belongs to biological medicine technology field, be specifically related to a kind of preparation method of spirulina extract.
Background technology
Spirullina is in Cyanophyta, Oscillariaceae, and Spirullina, is the spiral-shaped things of a kind of many cells.The various aminoacid useful to health such as its rich in proteins, mineral, vitamin and beta-carotene.Modern study finds, spirulina has the immunity of organisms, antioxidation, blood fat reducing, blood pressure lowering, promotion protein synthesis of raising and the physiological function such as anticancer.It was recommended by FAO (Food and Agriculture Organization of the United Nation) in 1974 be " the optimal food of 21 century ", World Health Organization (WHO) is decided to be " optimum medicines of mankind's 21 century ", U.S. food in 1981 and drug administration confirm as " one of optimum protein matter source ", and China's Ministry of Public Health is also ratified it for " new resource food ".
Along with the raising of living standards of the people, enriching of health knowledge, social increasing to the demand of health food, safety is also had higher requirement simultaneously.
Have bibliographical information, the growing environment of spirulina is easy to be subject to the pollution of other poisonous blue-green alge, particularly Microcystis aeruginosa.In 2002, the production status of Jiangsu, Yunnan, Fujian and Guangdong main spirulina production base is investigated, gathered spirulina manufacturing enterprise source water, breeding water, algae slurry, algae powder and portioned product.Result shows, except source water (tap water), all detects to some extent Microcystin.
Microcystin (MC) is the secondary metabolite being produced by some kind of the microcystis kutz in cyanophyceae, Anabaena, Oscillatoria and nostoc or strain.MC is owing to having the two keys in circulus and interval, and good stability, is difficult to decompose.Pertinent literature report, MC is a kind of hepatotoxin, can damage the Placenta Hominis of pregnant Mus, teratogenesis; Damage liver, the kidney of tire Mus period of embryo, form the basis of Liver Cancer.The ability that algae toxin can also make phagocytic activity, T lymphopoiesis and the bone-marrow-derived lymphocyte of macrophage produce antibody reduces, the kill capability of reduction to tumor cell etc.Therefore, remove MC in spirulina imperative.
Summary of the invention
The object of this invention is to provide a kind of preparation method that can effectively remove the spirulina extract of Microcystin.
The technical solution used in the present invention is a kind of preparation method of spirulina extract, comprises the following steps:
A, breaking cellular wall: get spirulina, be crushed to 600～800 orders, obtain breaking wall of spirullina princeps powder;
B, fermentation: described breaking wall of spirullina princeps powder is placed in to the fermentation liquid that is rich in Armillaria mellea and ferments 6～10 hours;
C, dry: adopt drying device to be dried, obtain xeraphium;
D, extraction: by described xeraphium water extraction, the residue after water extraction adopts supercritical CO again
2extract, obtain respectively water extract and extract.
In the present invention, described in be rich in Armillaria mellea fermentation liquid refer to that Armillaria mellea accounts for percentage by weight >=15% of whole fermentation liquid.
Preferably, in described step b, by weight, described fermentation liquid comprises 5～10 parts of 80～100 parts, water, 40～60 parts of Armillaria mellea, 5～10 parts of Cordyceps myceliums, 5～10 parts of nutrient solution of ginseng and lactic acid.
Particularly preferred, by weight, described fermentation liquid is made up of 6 parts of 90 parts, water, 50 parts of Armillaria mellea, 6 parts of Cordyceps myceliums, 6 parts of nutrient solution of ginseng and lactic acid.
Wherein, described nutrient solution of ginseng is by Radix Ginseng through water extraction, and condensed water extract makes.Concrete, Radix Ginseng adopts 10 times of decoctings to boil 3 times, merges decoction liquor, filters, and concentrated, obtaining proportion is 1.05g/cm
3nutrient solution of ginseng.
In the present invention, Armillaria mellea used, Cordyceps mycelium and lactic acid are all purchased the supplier of own legal qualification.
The preparation method of described fermentation liquid: Armillaria mellea and Cordyceps mycelium are soaked in water 8～12 hours, filter, obtain filtrate, then add nutrient solution of ginseng and lactic acid.
In the present invention, the residue after water extraction is the supercritical CO that adopts existing routine
2equipment extracts.
Preferably, in described step b, the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:5～10.Particularly preferred, the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:6, and the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:7, and the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:8, and the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:9.
It is preferably, dry in described step c that to be selected from spraying dry.
Preferably, in described steps d, the number of times of water extraction is 1～5 time, and the time of water extraction is 1～10 hour, the weight that adds water is described xeraphium 5～20 times.Particularly preferred, the number of times of water extraction is 2 times, 3 times and 4 times, and the time of water extraction is 2 hours, 4 hours, 6 hours and 8 hours, the weight that adds water is described xeraphium 8 times, 10 times, 12 times, 14 times, 16 times and 18 times.
The water extract that the present invention also provides a kind of described preparation method to make.
It is the pharmaceutical formulation that active component is made that the present invention also provides a kind of described water extract, the pharmaceutical formulation that the preparation method that described water extract adds medical additive to allow according to pharmaceutics is made.
The extract that the present invention also provides a kind of described preparation method to make.
It is the pharmaceutical formulation that active component is made that the present invention also provides a kind of described extract, the pharmaceutical formulation that the preparation method that described extract adds medical additive to allow according to pharmaceutics is made.
The above-mentioned medical additive of the present invention comprises saccharide, starch based, cellulose and derivant thereof, gelatin, Pulvis Talci, excipient, oils etc.
The above-mentioned pharmaceutical formulation of the present invention comprises the solid preparations such as tablet, capsule, granule, also comprises the liquid preparation such as oral liquid, injection, also comprises patch, ointment, gel etc.
When gained water extract of the present invention or extract are as medicament administration during in human or animal, they can give with itself, do not add any above-mentioned pharmaceutically acceptable carrier, and water extract above-mentioned the present invention or extract are directly applied to patient with original shape; Or can be for example to contain 0.1%～99.9% water extract or the pharmaceutical formulation of extract and the acceptable medical additive combination of pharmacy gives.
The raw material that gained water extract of the present invention or extract also can be used as health food, bread and cheese or cosmetics uses.
Beneficial effect of the present invention is:
(1) can effectively degrade and remove the Microcystin in spirulina, after testing, content≤the 90pg/g of Microcystin in the spirulina extract (water extract or extract) obtaining through the inventive method, well below the content (380ng/g) of Microcystin in existing spirulina goods.
(2), through safety testing evaluation, spirulina extract (water extract or the extract) safety non-toxic making through the inventive method, can eat for a long time.
(3) spirulina extract (water extract or extract) obtaining through the inventive method is rich in various active composition, has the effect such as enhancing immunity, antioxidation.
(4) preparation method of the present invention can also effectively be removed the heavy metal in spirulina.
Detailed description of the invention
For making those skilled in the art understand in detail production technology of the present invention and technique effect, further introduce application of the present invention and technique effect with concrete production instance below.
Embodiment 1:
A, breaking cellular wall: get spirulina, be crushed to 700 orders, obtain breaking wall of spirullina princeps powder;
B, fermentation: breaking wall of spirullina princeps powder is placed in to the fermentation liquid that is rich in Armillaria mellea and ferments 8 hours; The weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:8;
C, dry: adopt drying device to spray dry, obtain xeraphium;
D, extraction: by xeraphium water extraction, wherein, the number of times of water extraction is 3 times, each time of extracting is 6 hours, the weight that at every turn adds water is xeraphium 8 times.Residue after water extraction adopts conventional supercritical CO again
2equipment extracts, and obtains respectively water extract and extract.
Wherein, nutrient solution of ginseng is by Radix Ginseng through water extraction, and condensed water extract makes.Concrete, Radix Ginseng adopts 10 times of decoctings to boil 3 times, merges decoction liquor, filters, and concentrated, obtaining proportion is 1.05g/cm
3nutrient solution of ginseng.
The preparation of fermentation liquid: by weight, take 50 parts of Armillaria mellea and 90 parts of water soakings of 6 parts of use of Cordyceps mycelium 10 hours, filter, obtain filtrate, then add 6 parts of 6 parts of nutrient solution of ginseng and lactic acid.
Armillaria mellea, Cordyceps mycelium and the lactic acid of above-mentioned employing is all purchased the supplier of own legal qualification.
Embodiment 2:
A, breaking cellular wall: get spirulina, be crushed to 600 orders, obtain breaking wall of spirullina princeps powder;
B, fermentation: breaking wall of spirullina princeps powder is placed in to the fermentation liquid that is rich in Armillaria mellea and ferments 10 hours; The weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:5;
C, dry: adopt drying device to spray dry, obtain xeraphium;
D, extraction: by xeraphium water extraction, wherein, the number of times of water extraction is 5 times, each time of extracting is 1 hour, the weight that at every turn adds water is xeraphium 5 times.Residue after water extraction adopts conventional supercritical CO again
2equipment extracts, and obtains respectively water extract and extract.
Wherein, nutrient solution of ginseng is by Radix Ginseng through water extraction, and condensed water extract makes.Concrete, Radix Ginseng adopts 10 times of decoctings to boil 3 times, merges decoction liquor, filters, and concentrated, obtaining proportion is 1.05g/cm
3nutrient solution of ginseng.
The preparation of fermentation liquid: by weight, take 40 parts of Armillaria mellea and 100 parts of water soakings of 10 parts of use of Cordyceps mycelium 8 hours, filter, obtain filtrate, then add 10 parts of 5 parts of nutrient solution of ginseng and lactic acid.
Armillaria mellea, Cordyceps mycelium and the lactic acid of above-mentioned employing is all purchased the supplier of own legal qualification.
Embodiment 3:
A, breaking cellular wall: get spirulina, be crushed to 800 orders, obtain breaking wall of spirullina princeps powder;
B, fermentation: breaking wall of spirullina princeps powder is placed in to the fermentation liquid that is rich in Armillaria mellea and ferments 6 hours; The weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:10;
C, dry: adopt drying device to spray dry, obtain xeraphium;
D, extraction: by xeraphium water extraction, wherein, the number of times of water extraction is 1 time, and the time of extraction is 10 hours, the weight that adds water is xeraphium 20 times.Residue after water extraction adopts conventional supercritical CO again
2equipment extracts, and obtains respectively water extract and extract.
Wherein, nutrient solution of ginseng is by Radix Ginseng through water extraction, and condensed water extract makes.Concrete, Radix Ginseng adopts 10 times of decoctings to boil 3 times, merges decoction liquor, filters, and concentrated, obtaining proportion is 1.05g/cm
3nutrient solution of ginseng.
The preparation of fermentation liquid: by weight, take 60 parts of Armillaria mellea and 80 parts of water soakings of 5 parts of use of Cordyceps mycelium 12 hours, filter, obtain filtrate, then add 5 parts of 10 parts of nutrient solution of ginseng and lactic acid.
Armillaria mellea, Cordyceps mycelium and the lactic acid of above-mentioned employing is all purchased the supplier of own legal qualification.
Embodiment 4:
By 2000 grams of embodiment 1 gained water extract, add appropriate amount of starch, technique tabletting routinely, makes the tablet of 0.3 gram/piece.Oral, every day 3 times, each 1-2 sheet, for antioxidation and enhancing immunity.
Embodiment 5:
By 2000 grams of embodiment 1 gained extracts, add appropriate amount of starch, technique tabletting routinely, makes the tablet of 0.3 gram/piece.Oral, every day 3 times, each 1-2 sheet, for antioxidation and enhancing immunity.
Embodiment 6:
By 500 grams of 1000 grams of embodiment 1 gained water extract and extracts, mix, add appropriate micropowder silica gel, process filling routinely, makes the capsule of 0.25 gram/.Oral, every day 3 times, each 1-2 sheet, for antioxidation and enhancing immunity.
Embodiment 7:
By 500 grams of 500 grams of embodiment 1 gained water extract and extracts, then add suitable quantity of water and glycerol, make water replenishing cream, for increasing skin elasticity, skin moistening moisturizing, play the effect of protection skin.
Comparative example 1-4:
Except Spirulina powder being broken to 120 orders, 200 orders, 1000 orders and 1200 orders, all the other modes identical with embodiment 1 are implemented, and obtain comparative example 1-4.
Comparative example 5:
Except step b is omitted, all the other modes identical with embodiment 1 are implemented, and obtain comparative example 5.
For verifying technique effect of the present invention, inspection below special work:
As seen from the above table, there were significant differences for preparation method acquired results of the present invention and comparative example preparation method acquired results, and the importance of preparation method of the present invention has been described.
Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to above-described embodiment, those skilled in the art are to be understood that, still can modify or be equal to replacement the present invention, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in claim scope of the present invention.
Claims (10)
1. a preparation method for spirulina extract, is characterized in that: comprise the following steps:
A, breaking cellular wall: get spirulina, be crushed to 600～800 orders, obtain breaking wall of spirullina princeps powder;
B, fermentation: described breaking wall of spirullina princeps powder is placed in to the fermentation liquid that is rich in Armillaria mellea and ferments 6～10 hours;
C, dry: adopt drying device to be dried, obtain xeraphium;
D, extraction: by described xeraphium water extraction, the residue after water extraction adopts supercritical CO again
2extract, obtain respectively water extract and extract.
2. the preparation method of a kind of spirulina extract according to claim 1, it is characterized in that: in described step b, by weight, described fermentation liquid comprises 5～10 parts of 80～100 parts, water, 40～60 parts of Armillaria mellea, 5～10 parts of Cordyceps myceliums, 5～10 parts of nutrient solution of ginseng and lactic acid.
3. the preparation method of a kind of spirulina extract according to claim 2, is characterized in that: by weight, described fermentation liquid is made up of 6 parts of 90 parts, water, 50 parts of Armillaria mellea, 6 parts of Cordyceps myceliums, 6 parts of nutrient solution of ginseng and lactic acid.
4. the preparation method of a kind of spirulina extract according to claim 1, is characterized in that: in described step b, the weight ratio of breaking wall of spirullina princeps powder and fermentation liquid is 1:5～10.
5. the preparation method of a kind of spirulina extract according to claim 1, is characterized in that: dry in described step c to be selected from spraying dry.
6. the preparation method of a kind of spirulina extract according to claim 1, is characterized in that: in described steps d, the number of times of water extraction is 1～5 time, and the time of water extraction is 1～10 hour, the weight that adds water is described xeraphium 5～20 times.
7. the water extract making according to preparation method described in claim 1-6 any one.
8. a pharmaceutical formulation that utilizes the water extract described in claim 7 to make for active component, is characterized in that: the pharmaceutical formulation that the preparation method that described water extract adds medical additive to allow according to pharmaceutics is made.
9. the extract making according to preparation method described in claim 1-5 any one.
10. a pharmaceutical formulation that utilizes the extract described in claim 9 to make for active component, is characterized in that: the pharmaceutical formulation that the preparation method that described extract adds medical additive to allow according to pharmaceutics is made.
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| CN201410087576.6A CN103830280B (en) | 2014-03-11 | 2014-03-11 | Preparation method of spirulina extract |
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| CN201410087576.6A CN103830280B (en) | 2014-03-11 | 2014-03-11 | Preparation method of spirulina extract |
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| CN103830280A true CN103830280A (en) | 2014-06-04 |
| CN103830280B CN103830280B (en) | 2017-03-22 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178427A (en) * | 2014-08-25 | 2014-12-03 | 云南天保桦生物资源开发有限公司 | Method for removing microcystin from spirulina |
| CN106074624A (en) * | 2016-07-28 | 2016-11-09 | 南京正宽医药科技有限公司 | A kind of spirulina extracting method and the health tea for the treatment of hyperlipemia |
| CN107308028A (en) * | 2017-04-01 | 2017-11-03 | 上海乐谷娜生物科技有限公司 | Cutaneous penetration essence |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRMU9102088U2 (en) * | 2011-08-25 | 2016-04-26 | Univ Fed Pernambuco | use of a protein nanopore for detection, identification, quantification and real time monitoring of microcystins in aqueous systems |
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
-
2014
- 2014-03-11 CN CN201410087576.6A patent/CN103830280B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178427A (en) * | 2014-08-25 | 2014-12-03 | 云南天保桦生物资源开发有限公司 | Method for removing microcystin from spirulina |
| CN104178427B (en) * | 2014-08-25 | 2016-09-28 | 云南天保桦生物资源开发有限公司 | A kind of remove the method for Microcystin in spirulina |
| CN106074624A (en) * | 2016-07-28 | 2016-11-09 | 南京正宽医药科技有限公司 | A kind of spirulina extracting method and the health tea for the treatment of hyperlipemia |
| CN107308028A (en) * | 2017-04-01 | 2017-11-03 | 上海乐谷娜生物科技有限公司 | Cutaneous penetration essence |
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