CN103830280B - Preparation method of spirulina extract - Google Patents
Preparation method of spirulina extract Download PDFInfo
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- CN103830280B CN103830280B CN201410087576.6A CN201410087576A CN103830280B CN 103830280 B CN103830280 B CN 103830280B CN 201410087576 A CN201410087576 A CN 201410087576A CN 103830280 B CN103830280 B CN 103830280B
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Abstract
The invention relates to a preparation method of a spirulina extract, belonging to the technical field of biological medicine. The method comprises the following steps: (a) wall breaking: grinding spirulina to 600-800 meshes to obtain spirulina broken-wall powder; (b) fermentation: fermenting the spirulina broken-wall powder in amillariella mellea-rich fermentation liquid for 6-10 hours; (c) drying: drying with a drying device to obtain dry powder; (d) extraction: extracting the dry powder with water, and extracting a water extract and an extract respectively from the residue after the water extraction by use of supercritical CO2. The method provided by the invention has the beneficial effects that microcystin in the spirulina can be effectively degraded and removed; according to detection, the content of microcystin in the spirulina extract obtained by the method is less than or equal to 90pg/g, which is much lower than the content of microcystin in the existing spirulina products.
Description
Technical field
The invention belongs to biomedicine technical field, and in particular to a kind of preparation method of spirulina extract.
Background technology
Spirullina, in Cyanophyta, Oscillariaceae, Spirullina, is a kind of many cells spiral-shaped thing.Its rich in proteins,
The various aminoacid useful to health such as mineral, vitamin and beta-carotene.Modern study finds that spirulina has
Improve immunity of organisms, antioxidation, blood fat reducing, blood pressure lowering, the promotion physiological function such as protein synthesis and anticancer.The United Nations grain farmer
Be organized in and recommend within 1974 it for " the optimal food of 21 century ", World Health Organization (WHO) be set to " mankind's 21 century it is optimal
Health product ", United States food and drag administration confirms as " one of optimum protein matter source " within 1981, and China's Ministry of Public Health is also criticized
It is accurate its be " new resource food ".
With the raising of living standards of the people, enriching for health knowledge is social increasing to the demand of health food, together
When it is also proposed higher requirement to safety.
There is document report, the growing environment of spirulina is highly susceptible to the dirt of other poisonous blue-green alge, particularly Microcystis aeruginosas
Dye.Investigated in the production status to the main spirulina production base in Jiangsu, Yunnan, Fujian and Guangdong in 2002, gathered
Spirulina manufacturing enterprise source water, breeding water, algae slurry, algae powder and portioned product.As a result show, except source water（Tap water）
Outward, Microcystin is detected to some extent.
Microcystin（MC）It is by some kinds of the microcystis kutz in cyanophyceae, Anabaena, Oscillatoria and nostoc
The secondary metabolite that class or strain are produced.MC is due to circulus and interval double bond, good stability, it is difficult to decompose.Phase
Document report is closed, MC is a kind of hepatotoxin, can damage the Placenta Hominiss of pregnant Mus, teratogenesis；Liver, the kidney of tire Mus, shape are damaged in period of embryo
Into the basis of Liver Cancer.Algae toxins produce can also the phagocytic activity of macrophage, T lymphopoiesis and bone-marrow-derived lymphocyte
The ability of raw antibody is reduced, and reduces killing ability to tumor cell etc..Therefore, the MC for removing in spirulina is imperative.
The content of the invention
It is an object of the invention to provide a kind of preparation method of the spirulina extract that can effectively remove Microcystin.
The technical solution used in the present invention is a kind of preparation method of spirulina extract, is comprised the following steps：
A, breaking cellular wall：Spirulina is taken, 600～800 mesh are crushed to, breaking wall of spirullina princeps powder is obtained；
B, fermentation：The breaking wall of spirullina princeps powder is placed in the fermentation liquid rich in Armillaria mellea carries out fermentation 6～10 hours；
C, drying：It is dried using drying device, obtains xeraphium；
D, extraction：By the xeraphium water extraction, the residue after water extraction adopts supercritical CO again2Extracted, point
Water extract and extract are not obtained.
In the present invention, the fermentation liquid rich in Armillaria mellea refers to that Armillaria mellea accounts for percentage by weight >=15% of whole fermentation liquid.
Preferably, in step b, by weight, the fermentation liquid includes 80～100 parts of water, Armillaria mellea 40～60
Part, 5～10 parts of Cordyceps mycelium, 5～10 parts of nutrient solution of ginseng and 5～10 parts of lactic acid.
Particularly preferred, by weight, the fermentation liquid is by 90 parts of water, 50 parts of Armillaria mellea, 6 parts of Cordyceps mycelium, people
6 parts of compositions of 6 parts of ginseng nutritional solution and lactic acid.
Wherein, by Radix Ginseng through water extraction, concentration Aqueous extracts are obtained the nutrient solution of ginseng.Specifically, Radix Ginseng adopts 10 times
Decocting is boiled 3 times, merges decoction liquor, is filtered, concentration, obtains proportion for 1.05g/cm3Nutrient solution of ginseng.
In the present invention, Armillaria mellea, Cordyceps mycelium and lactic acid used purchase the supplier of own legal qualification.
The preparation method of the fermentation liquid：Armillaria mellea and Cordyceps mycelium are soaked in water 8～12 hours, are filtered, was obtained
Filtrate, adds nutrient solution of ginseng and lactic acid, you can.
In the present invention, the residue after water extraction is using existing conventional supercritical CO2Equipment is being extracted.
Preferably, in step b, breaking wall of spirullina princeps powder is 1 with the weight ratio of fermentation liquid:5～10.It is particularly preferred, spiral shell
Rotation algae wall cell disruption powder is 1 with the weight ratio of fermentation liquid:6, breaking wall of spirullina princeps powder is 1 with the weight ratio of fermentation liquid:7, breaking wall of spirullina princeps
Powder is 1 with the weight ratio of fermentation liquid:8, breaking wall of spirullina princeps powder is 1 with the weight ratio of fermentation liquid:9.
Preferably, it is dried selected from spray drying in step c.
Preferably, in step d, the number of times of water extraction is 1～5 time, and the time of water extraction is 1～10 hour, adds water
Weight be 5～20 times of the xeraphium.Particularly preferred, the number of times of water extraction is 2 times, 3 times and 4 times, water extraction when
Between be 2 hours, 4 hours, 6 hours and 8 hours, the weight for adding water is 8 times of the xeraphium, 10 times, 12 times, 14 times, 16
Again with 18 times.
The present invention also provides water extract obtained in a kind of preparation method.
It is pharmaceutical formulation made by active component that the present invention also provides a kind of described water extract, and the water extract adds
Enter pharmaceutical formulation made by the preparation method that medical additive is allowed according to pharmaceuticss.
The present invention also provides extract obtained in a kind of preparation method.
It is pharmaceutical formulation made by active component that the present invention also provides a kind of described extract, and described extract is added
Pharmaceutical formulation made by the preparation method that medical additive is allowed according to pharmaceuticss.
The above-mentioned medical additive of the present invention includes saccharide, starch based, cellulose and its derivates, gelatin, Pulvis Talci, figuration
Agent, oils etc..
The above-mentioned pharmaceutical formulation of the present invention includes the solid preparations such as tablet, capsule, granule, also including oral liquid, injection
The liquid preparations such as agent, also including patch, ointment, gel etc..
When water extract obtained by the present invention or extract as medicament administration in human or animal when, they can be with itself
Give, that is, be not added with any above-mentioned pharmaceutically acceptable carrier, will be the present invention above-mentioned water extract or extract straight with original shape
Connect and be applied to patient；Or can be with containing such as 0.1%～99.9% water extract or extract with it is pharmaceutically acceptable
The pharmaceutical formulation of medical additive combination gives.
Gained water extract of the invention or extract are alternatively arranged as the raw material of health food, bread and cheese or cosmetics to be carried out
Use.
The beneficial effects of the present invention is：
（1）The Microcystin that effectively can be degraded in removing spirulina, after testing, the spirulina that Jing the inventive method is obtained
Extract（Water extract or extract）Content≤the 90pg/g of middle Microcystin, it is micro- well below in existing spiral algae product
The content of capsule Algae toxins（380ng/g）.
（2）Jing safety testings are evaluated, spirulina extract obtained in Jing the inventive method（Water extract or extract）
Safety non-toxic, can be eaten for a long time.
（3）The spirulina extract that Jing the inventive method is obtained（Water extract or extract）Rich in various active composition,
The effects such as with enhancing immunity, antioxidation.
（4）Preparation method of the present invention can also effectively remove the heavy metal in spirulina.
Specific embodiment
For making those skilled in the art understand production technology and the technique effect of the present invention in detail, below specifically producing
Example come be further described the present invention application and technique effect.
Embodiment 1：
A, breaking cellular wall：Spirulina is taken, 700 mesh are crushed to, breaking wall of spirullina princeps powder is obtained；
B, fermentation：Breaking wall of spirullina princeps powder is placed in the fermentation liquid rich in Armillaria mellea carries out fermentation 8 hours；Breaking wall of spirullina princeps
Powder is 1 with the weight ratio of fermentation liquid:8；
C, drying：It is spray-dried using drying device, is obtained xeraphium；
D, extraction：By xeraphium water extraction, wherein, the number of times of water extraction is 3 times, and the time extracted every time is 6 hours,
The weight for adding water every time is 8 times of xeraphium.Residue after water extraction adopts conventional Supercritical CO again2Equipment is being extracted
Take, respectively obtain water extract and extract.
Wherein, by Radix Ginseng through water extraction, concentration Aqueous extracts are obtained nutrient solution of ginseng.Specifically, Radix Ginseng adopts 10 times of decoctings
Boil 3 times, merge decoction liquor, filter, concentration obtains proportion for 1.05g/cm3Nutrient solution of ginseng.
The preparation of fermentation liquid：By weight, weigh 50 parts of Armillaria mellea and the immersion 10 of 6 parts of Cordyceps mycelium, 90 parts of use water is little
When, filter, obtain filtrate, add 6 parts of 6 parts of nutrient solution of ginseng and lactic acid, you can.
The Armillaria mellea of above-mentioned employing, Cordyceps mycelium and lactic acid purchase the supplier of own legal qualification.
Embodiment 2：
A, breaking cellular wall：Spirulina is taken, 600 mesh are crushed to, breaking wall of spirullina princeps powder is obtained；
B, fermentation：Breaking wall of spirullina princeps powder is placed in the fermentation liquid rich in Armillaria mellea carries out fermentation 10 hours；Spirulina breaks
Wall powder is 1 with the weight ratio of fermentation liquid:5；
C, drying：It is spray-dried using drying device, is obtained xeraphium；
D, extraction：By xeraphium water extraction, wherein, the number of times of water extraction is 5 times, and the time extracted every time is 1 hour,
The weight for adding water every time is 5 times of xeraphium.Residue after water extraction adopts conventional Supercritical CO again2Equipment is being extracted
Take, respectively obtain water extract and extract.
Wherein, by Radix Ginseng through water extraction, concentration Aqueous extracts are obtained nutrient solution of ginseng.Specifically, Radix Ginseng adopts 10 times of decoctings
Boil 3 times, merge decoction liquor, filter, concentration obtains proportion for 1.05g/cm3Nutrient solution of ginseng.
The preparation of fermentation liquid：By weight, 40 parts of Armillaria mellea and 10 parts of Cordyceps mycelium, 100 parts of use water immersion 8 are weighed
Hour, filter, obtain filtrate, add 10 parts of 5 parts of nutrient solution of ginseng and lactic acid, you can.
The Armillaria mellea of above-mentioned employing, Cordyceps mycelium and lactic acid purchase the supplier of own legal qualification.
Embodiment 3：
A, breaking cellular wall：Spirulina is taken, 800 mesh are crushed to, breaking wall of spirullina princeps powder is obtained；
B, fermentation：Breaking wall of spirullina princeps powder is placed in the fermentation liquid rich in Armillaria mellea carries out fermentation 6 hours；Breaking wall of spirullina princeps
Powder is 1 with the weight ratio of fermentation liquid:10；
C, drying：It is spray-dried using drying device, is obtained xeraphium；
D, extraction：By xeraphium water extraction, wherein, the number of times of water extraction is 1 time, and the time of extraction is 10 hours, plus
The weight for entering water is 20 times of xeraphium.Residue after water extraction adopts conventional Supercritical CO again2Equipment being extracted, respectively
Obtain water extract and extract.
Wherein, by Radix Ginseng through water extraction, concentration Aqueous extracts are obtained nutrient solution of ginseng.Specifically, Radix Ginseng adopts 10 times of decoctings
Boil 3 times, merge decoction liquor, filter, concentration obtains proportion for 1.05g/cm3Nutrient solution of ginseng.
The preparation of fermentation liquid：By weight, weigh 60 parts of Armillaria mellea and the immersion 12 of 5 parts of Cordyceps mycelium, 80 parts of use water is little
When, filter, obtain filtrate, add 5 parts of 10 parts of nutrient solution of ginseng and lactic acid, you can.
The Armillaria mellea of above-mentioned employing, Cordyceps mycelium and lactic acid purchase the supplier of own legal qualification.
Embodiment 4：
By 2000 grams of 1 gained water extract of embodiment, appropriate amount of starch is added, routinely technique tabletting, makes 0.3 gram/piece
Tablet.Orally, 3 times a day, and 1 to 2 tablets once, for antioxidation and enhancing immunity.
Embodiment 5：
By 2000 grams of 1 gained extract of embodiment, appropriate amount of starch is added, routinely technique tabletting, makes 0.3 gram/piece
Tablet.Orally, 3 times a day, and 1 to 2 tablets once, for antioxidation and enhancing immunity.
Embodiment 6：
By 500 grams of 1000 grams of 1 gained water extract of embodiment and extract, mix, add appropriate micropowder silica gel, routinely
Process filling, makes the capsule of 0.25 gram/.Orally, 3 times a day, and 1 to 2 tablets once, for antioxidation and enhancing immunity.
Embodiment 7：
By 500 grams of 500 grams of 1 gained water extract of embodiment and extract, suitable quantity of water and glycerol is added, moisturizing is made
Frost, for increasing skin elasticity, skin moistening moisturizing, plays a part of to protect skin.
Comparative example 1-4：
In addition to Spirulina powder to be broken to 120 mesh, 200 mesh, 1000 mesh and 1200 mesh, remaining mode same as Example 1
Implemented, obtained comparative example 1-4.
Comparative example 5：
Except step b is omitted, remaining mode same as Example 1 is implemented, and obtains comparative example 5.
For the technique effect of the checking present invention, special work is following to be checked：
As seen from the above table, there were significant differences with comparative example preparation method acquired results for preparation method acquired results of the present invention,
Illustrate the importance of preparation method of the present invention.
Finally it should be noted that above example is only to illustrative and not limiting technical scheme, although reference
Above-described embodiment has been described in detail to the present invention, it will be appreciated by those skilled in the art that still can carry out to the present invention
Modification or equivalent, any modification or partial replacement without departing from the spirit and scope of the present invention, which all should be covered
In scope of the presently claimed invention.
Claims (9)
1. a kind of preparation method of spirulina extract, it is characterised in that：Comprise the following steps：
A, breaking cellular wall：Spirulina is taken, 600～800 mesh are crushed to, breaking wall of spirullina princeps powder is obtained；
B, fermentation：The breaking wall of spirullina princeps powder is placed in the fermentation liquid rich in Armillaria mellea carries out fermentation 6～10 hours；
C, drying：It is dried using drying device, obtains xeraphium；
D, extraction：By the xeraphium water extraction, the residue after water extraction adopts supercritical CO again2Extracted, respectively obtained
Water extract and extract；In step b, by weight, the fermentation liquid is by 80～100 parts of water, Armillaria mellea 40～60
Part, 5～10 parts of Cordyceps mycelium, 5～10 parts of nutrient solution of ginseng and 5～10 parts of compositions of lactic acid；The nutrient solution of ginseng is to pass through
Radix Ginseng is boiled 3 times using 10 times of decoctings, merges decoction liquor, filtered, concentration obtains proportion for 1.05g/cm3Nutrient solution of ginseng.
2. the preparation method of a kind of spirulina extract according to claim 1, it is characterised in that：By weight, institute
State fermentation liquid to be made up of 6 parts of 90 parts of water, 50 parts of Armillaria mellea, 6 parts of Cordyceps mycelium, 6 parts of nutrient solution of ginseng and lactic acid.
3. the preparation method of a kind of spirulina extract according to claim 1, it is characterised in that：Spiral shell in step b
Rotation algae wall cell disruption powder is 1 with the weight ratio of fermentation liquid:5～10.
4. the preparation method of a kind of spirulina extract according to claim 1, it is characterised in that：Do in step c
It is dry selected from spray drying.
5. the preparation method of a kind of spirulina extract according to claim 1, it is characterised in that：Water in step d
The number of times of extraction is 1～5 time, and the time of water extraction is 1～10 hour, and the weight for adding water is 5～20 times of the xeraphium.
6. water extract obtained in the preparation method according to any one of claim 1-5.
7. the water extract described in a kind of utilization claim 6 is pharmaceutical formulation made by active component, it is characterised in that：It is described
Water extract adds pharmaceutical formulation made by the preparation method that medical additive is allowed according to pharmaceuticss.
8. extract obtained in the preparation method according to any one of claim 1-4.
9. the extract described in a kind of utilization claim 8 is pharmaceutical formulation made by active component, it is characterised in that：Described
Extract adds pharmaceutical formulation made by the preparation method that medical additive is allowed according to pharmaceuticss.
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| CN201410087576.6A CN103830280B (en) | 2014-03-11 | 2014-03-11 | Preparation method of spirulina extract |
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| CN201410087576.6A CN103830280B (en) | 2014-03-11 | 2014-03-11 | Preparation method of spirulina extract |
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| CN103830280B true CN103830280B (en) | 2017-03-22 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178427B (en) * | 2014-08-25 | 2016-09-28 | 云南天保桦生物资源开发有限公司 | A kind of remove the method for Microcystin in spirulina |
| CN106074624A (en) * | 2016-07-28 | 2016-11-09 | 南京正宽医药科技有限公司 | A kind of spirulina extracting method and the health tea for the treatment of hyperlipemia |
| CN107308028A (en) * | 2017-04-01 | 2017-11-03 | 上海乐谷娜生物科技有限公司 | Cutaneous penetration essence |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
| WO2013026123A1 (en) * | 2011-08-25 | 2013-02-28 | Universidade Federal De Pernambuco - Ufpe | Use of a protein nanopore for the detection, identification, quantification and real-time monitoring of microcystins in aqueous systems |
-
2014
- 2014-03-11 CN CN201410087576.6A patent/CN103830280B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013026123A1 (en) * | 2011-08-25 | 2013-02-28 | Universidade Federal De Pernambuco - Ufpe | Use of a protein nanopore for the detection, identification, quantification and real-time monitoring of microcystins in aqueous systems |
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
Non-Patent Citations (2)
| Title |
|---|
| Microcystin Elimination During Sediment Contact;Gesche Grutzmacher,et al;《Environ.Sci.Technol》;20101211;第44卷(第2期);第657-662页 * |
| 饮用水中藻类及藻毒素去除技术进展;熊建功，等;《重庆工商大学学报（自然科学版）》;20120825;第29卷(第8期);第83页-第86页 * |
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