CN106983146A - Prepare the fermentation composition and preparation method of the plant enzyme with conditioning and enhancing human detoxification moistening face - Google Patents
Prepare the fermentation composition and preparation method of the plant enzyme with conditioning and enhancing human detoxification moistening face Download PDFInfo
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
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- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides the fermentation composition for preparing the plant enzyme with conditioning and enhancing human detoxification moistening face, it is made up of functional oligose, probiotics and plant extracts;Plant extracts is mainly prepared from by the raw material of following parts by weight:10~63 parts of anthocyanidin, 8~42 parts of rose and 10~50 parts of Cranberry.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", the industrialized production or family manufacture of suitable all kinds of plant enzymes.
Description
Technical field
The invention belongs to prevent conditioning maintenance product field, more particularly to prepared by one kind to support with conditioning and enhancing human detoxification
The fermentation composition and preparation method of face effect.
Background technology
Existing traditional handicraft and modern crafts to ferment is improved, occur in that some addition probiotics ferment and
Health food prepared by ferment.Probiotics fermention fruits and vegetables, can activate the effective efficiency composition in fruits and vegetables, the activity of dissolution more elite
Effect material.But, traditional ferment technique can not be used flexibly on a large scale, or without effect fermentation composition can be in difference
Flexibly used in the industrial manufacturing processes of ferment;Promoting the side such as metabolism, coordinating intestines and stomach function in vivo to improve ferment
The effect in face, and adapt to use on a large scale, applicant further improves to the fermentate for preparing ferment, and develops raising
The formula of the function affect of ferment.
There is physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degraded in addition, functional oligose is a class, do not inhaled in small intestine
Receive, reach the oligosaccharide of big intestines;Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, and with promoting, human body is prebiotic
The physiological functions such as the propagation of bacterium.By the way that probiotics and functional oligose are used in conjunction with, between the two with synergy, energy
Promote the regulatory function to gut flora.Co between probiotics and oligosaccharide, be probiotic in symphysis unit and
Co between prebiotics, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria
Growth;Research shows that both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no
This effect.Such as the enzyme composition that patent CN106174179A is related to, probiotics and functional oligose are used in combination, and
Traditional Chinese medicine ingredients are added, its given efficacy is reached.But its preparation being formulated is applied to individual and directly taken, and fixed plant enzyme
Stoste can not meet different taste Man's Demands when reusing, and its preparation method is coarse, in the ferment effervescent tablet being prepared into
The sense of the medicine dregs of a decoction is strong, and mouthfeel is not good.
In addition, existing ferment bucket, plant enzyme is prepared using fruit, sugar and water as raw material so that plant enzyme is in
Front yard can be prepared, easy to operate.But also having many weak points, the time prepared by 1. ferment is still longer, and easily makes
Standby failure;2. made from plant enzyme effect it is indefinite, user can not oneself control prepare effect of plant enzyme, such as it is right
Human body has the plant enzyme of toxin-expelling and face nourishing effect;3. the low molecular sugar and polysaccharide majority in the plants such as fruit can not be utilized,
The oligosaccharide of given efficacy can not be converted into.For the deficiency, applicant, which puts forth effort research, to be had shortening ferment preparation time, makes
With fermentation composition convenient and with conditioning and enhancing human detoxification moistening face, i.e. ferment bag.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention
It is to propose a kind of fermentation composition prepared with conditioning and enhancing human detoxification moistening face, is directly putting type fermented microbial inoculum,
Probiotics and functional oligose are used in combination the fermentation composition, while adding plant extracts composition, make said composition
While with good gut flora regulatory function, the conversion of small molecular sugar and polysaccharide to oligosaccharide can be promoted.With
Plant enzyme prepared by the fermentation composition plays the body detoxification of enhancing human body, and has effects that beauty treatment to human body.
Technical scheme is as follows:
It is a kind of to prepare the fermentation composition with the plant enzyme nursed one's health and strengthen human detoxification moistening face, it is direct putting type
Plant enzyme fermenting agent, the fermentation composition includes the component of following parts by weight:It is 3~22 parts of functional oligose, prebiotic
0.01~10 part of 0.1~5 part of bacterium and plant extracts;
The plant extracts is mainly prepared from by the raw material of following parts by weight:10~63 parts of anthocyanidin, 8~42 parts
Rose and 10~50 parts of Cranberry;
The preparation method of the fermentation composition at least comprises the steps:
The fine powder that the functional oligose and plant extracts of predetermined weight number are made uniformly mixes sterilization, after sterilization
It is well mixed, granulates with probiotic powder, the fermentation composition of powdery is made.
In the program, the functional oligose is low selected from chitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew
Glycan, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, sea
One or more in algae sugar, palatinose and inulin;
Probiotics is selected from Bifidobacterium (Bifidobacterium), Lactobacillus rhamnosus (L.rhamnosus), cheese breast
Bacillus (Lactobacillus casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus
Plantarum), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus
Helveticus), Bu Shi lactobacillus (Lactobacillus buchneri), lactobacillus bulgaricus
(Lactobacillus.Bulgaricus), lactobacillus paracasei (Lactobacillus paracasei) and the newborn bars of Luo Yishi
One or more in bacterium (Lactobacillus reuteri).
Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle
The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity;And anthocyanidin is pure natural
The nutritious supplementary pharmaceutical of anti-aging, is effective antioxidant, and it can maintain normal cell linkages, the stabilization of blood vessel, strengthen micro-
The flowing of thin vascular circulation, raising capilary and vein, and then reach the rapid healing of abnormal skin;Anthocyanidin or natural
Sunlight overcover, can prevent ultraviolet damage skin, and skin belongs to connective tissue, collagen and rigid egg contained therein
The white total to skin plays an important role.Coordinate the use of rose and Cranberry, further realize and nurse one's health and strengthen
To effect of the toxin-expelling and face nourishing of human body.The anthocyanidin that the present invention is used is the finished product that market is sold, the plant for extracting anthocyanidin
Thing is not limited, and blueberry anthocyanin is preferably used in the present invention.On the other hand, powdery, implementation phase formula is made in fermentation composition
Production, be easy to the later stage direct plunge into use or family oriented use.
Further, the plant extracts also raw material including following weight parts, which is extracted, forms:2~20 parts of matrimony vine, 1
~20 parts of peach kernel, 3~20 parts of mulberries, 1~16 part of chrysanthemum, 3~25 parts of cassia seed, 3~25 parts of Poria cocos, 4~26
The root of kudzu vine, 1~20 part of the root of Dahurain angelica, 3~20 parts of dark plum and 2~14 parts of sea-buckthorn of part.
Further, functional oligose of the fermentation composition by parts by weight for 5~10 parts, 1~3 part prebiotic
Bacterium and 0.5~1 part of plant extracts extract composition;
The plant extracts is extracted by the raw material of following parts by weight and formed:25~40 parts of anthocyanidin, 10~26 parts
Rose, 14~22 parts of Cranberry, 6~13 parts of matrimony vine, 5~10 parts of peach kernel, 6~14 parts of mulberries, 5~10 parts of chrysanthemum
Flower, 9~16 parts of cassia seed, 9~16 parts of Poria cocos, 12~18 parts of the root of kudzu vine, 6~11 parts of the root of Dahurain angelica, 6~12 parts of dark plum with
And 5~9 parts of sea-buckthorn.
The preferred scheme of the fermentation composition of the present invention, in addition to the resistant dextrin that parts by weight are 10~30 parts, resistance
Dextrin is used in conjunction with other components, further promotes intestines peristalsis, keeps intestinal health, and control the absorption of grease and sugar.
Further, the functional oligose is trehalose;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:Bifidobacterium 45~
60 parts, 50~60 parts of Lactobacillus rhamnosus, 22~30 parts of Lactobacillus casei, 18~26 parts of lactobacillus acidophilus and Lactobacillus plantarum
23~30 parts, the viable bacteria content of the probiotics powder agent is 2 × 106~2 × 1010cfu/g;By all kinds of strains of reasonable disposition and
Consumption, optimizes the strain of this patent products'texture, can effectively shorten fermentation composition in the time for preparing plant enzyme, and can make
It prepares the stable plant enzyme of texture.
The source of above-mentioned all kinds of probiotics powder agents:It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of
Strain is collected by centrifugation with after washing thalline, drying is prepared into active powder through Liquid Culture.
In the one of preferred scheme of fermentation composition of the present invention, it is 1 that the fermentation composition, which also includes parts by weight,
~3 parts of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight
Extrusion is carried out afterwards and makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the parts by weight
It is even, softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition;
Wherein, the coating solution is by 100-55,0.1-2 parts of Eudragit L that parts by weight are 0.4-10 parts
Hydroxypropyl Methylcellulose Phathalate and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
It is a further object to provide a kind of preparation method of above-mentioned fermentation composition, methods described includes following
Step:
1) preparation of plant extracts:
The peach kernel, Poria cocos and the root of kudzu vine of the parts by weight are crushed respectively, 60 mesh sieves are crossed, ox Peach Seed, kudzu-vine root powder is made
End and Indian Bread;
By the rose, Cranberry, matrimony vine, mulberry fruit, chrysanthemum of Peach Seed, root of kudzu vine powder, Indian Bread and predetermined weight part
The water of 8 times of weight of flower, cassia seed, the root of Dahurain angelica, dark plum, sea-buckthorn, 4h is kept at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus
The alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering obtains the first filtrate;The water of 3 times of weight of filter residue is added, in temperature 80-90
2.5h is kept at DEG C, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, low temperature powder
Coarse powder is broken into, the fine powder that the follow-up continued powder of anthocyanidin is broken into 0.05-0.2mm is added;
2) by step 1) in fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear,
Wall material monomer is added dropwise while stirring and carries out wall building, nano level particulate is formed;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through
Heat exchanger is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery is made, and sterile packing is produced.
Fermentation composition viable bacteria rate prepared by the above method is high, the traditional Chinese medicine fine powder delicate mouthfeel of particulate processing, Chinese medicine particulate
The capsule heart is slowly discharged, with good slow release effect by processes such as dissolving, infiltration, diffusions through membranous wall.
A further object of the present invention is to provide a kind of plant enzyme with conditioning and enhancing human detoxification moistening face,
Formulated for personal or Homemade plant enzyme, the preparation method of the plant enzyme comprises the following steps:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5
~6L pure water, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket
Interior, capping carries out lucifuge fermentation in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering, i.e.,
Obtain plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and this method preferably uses ferment bucket and made
Standby, ferment bucket is from market sale;Also the vessel of closed environment can be used to be prepared.
Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Which is applied to industrial production, with reference to the secondary of plant enzyme stoste first fermentation and fermentation composition of the present invention
Multistage deep layer liquid state fermentation is realized in fermentation;Greatly improve fermentation efficiency.It can release in fermentation process and be enclosed in Chinese medicine or plant
Structure and intracellular nutrients element, retain the original active material of plant, insoluble fibre is changed into solvable in plant
Property dietary fiber improve effect of original plant.
The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition also constructed for double-deck pellet
Method, the preparation method comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation
Realize second order fermentation, or even multistage fermentation;The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries
Its high effect.
The present invention has the following advantages that compared with prior art:
The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing
Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly be existed with the bacterium bag of granule
Sell on the market, can also put into the industrialized production of all kinds of plant enzymes, realize that matching somebody with somebody for plant enzyme is used and diversification
Functional configuration, is greatly enhanced the preparation efficiency of plant enzyme.
The plant extracts component added in the fermentation composition of the present invention has its physiologically active and health-care effect, simultaneously
Supplemented the nutrients for probiotics in fermentation, with higher practical value, and the plant extracts composition of the present invention improves body
Effect of toxin-expelling and face nourishing;Rationally, curative effect is clear and definite for compatibility between probiotics, functional oligose and plant extracts each component,
Effect with Synergistic.
The plant enzyme of the present invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body
The comprehensive plant enzyme of benefit, metabolism and function adjustment of these enzymes to human body plays an important role, while also containing vitamin, mineral
The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group
Plant extracts composition in compound is changed through probiotics, is become small molecule by macromolecular, is easily absorbed by the body;Probiotics
Addition, which can improve, can decompose toxicity in plant extracts taste, fermentation process, realization really has no toxic side effect, while the fermentation
Plant enzyme prepared by composition improves drug effect compared to traditional plant extracts.The plant enzyme is used for conditioning engine body
Toxin expelling, it takes as daily drinks, improves lipid-metabolism from the angle effected a permanent cure, realizes the purpose of toxin-expelling and face nourishing and health care.
In addition, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big
Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.
The source of the plant extracts material composition of the present invention:
Rose:This product is rosaceous plant rose Rosa rugosa Thunb. dry flower.Cranberry:For cuckoo
Flower section Vaccinium crowberry subgenus (scientific name:Oxycoccos, also known as littlefruit craneberry subgenus) be commonly called as, the species of this subgenus are
In evergreen shrubs, the nice and cool area acid peat soil earth of main growth on the Northern Hemisphere.
Matrimony vine:This product is plant of Solanaceae lycium barbarum Lycium barbarum L. dry mature fruit.
Peach kernel:This product is rosaceous plant peach Prunus persica (L.) Batsch or mountain peach Prunus davidiana
(Carr.) Franch. dry mature seed.
Mulberries:This product is moraceae plants mulberry Morus alba L. drying fruit ear.
Chrysanthemum:This product is feverfew chrysanthemum Chrysanthemum morifolium Ramat. dry capitulum.
Cassia seed:This product is legume Cassia Cassia obtusifolia L. or little Cassia tora Cassia tora L.
Dry mature seed.
Poria cocos:This product is On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf dry sclerotia.
The root of kudzu vine:This product is legume pueraria lobata Pueraria lobata (Willd.) Ohwi or sweet kudzu Pueraria
Thomsonii Benth. dry root.
The root of Dahurain angelica:This product is umbellate form section the plant root of Dahurain angelica Angelica dahurica (Fisch.ex Hoffm.) Benth.et
Or Radix angelicae dahuricae Angelica dahurica (Fisch.ex Hoffm.) Benth.et Hook.f.
Hook.f.var.formosana (Boiss.) Shan et Yuan dry root.
Dark plum:This product is rose department plant plum Prunus mume (Sieh.) the Sieb.et Zucc. nearly mellow fruit of drying
It is real.
Sea-buckthorn:For Elaeangnaceae hippophae plant sea-buckthorn Hippophae rhamnoides L. dry mature fruit.
Embodiment
The fermentation composition of embodiment 1
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 80g
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 5 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
2.3 parts of 2.2 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:8g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin 2.5
Part, 1 part of rose, 1.4 parts of Cranberry, 0.6 part of matrimony vine, 0.5 part of peach kernel, 0.6 mulberries part, 0.5 part of chrysanthemum, 0.9 part of cassia seed,
0.5 part of 0.9 part of Poria cocos, 1.2 parts of the root of kudzu vine, 0.6 part of the root of Dahurain angelica, 0.6 part of dark plum and sea-buckthorn;
Resistant dextrin 80g.
Preparation method:
1) preparation of plant extracts:
The peach kernel, Poria cocos and the root of kudzu vine of the parts by weight are crushed respectively, 60 mesh sieves are crossed, ox Peach Seed, kudzu-vine root powder is made
End and Indian Bread;
By the rose, Cranberry, matrimony vine, mulberry fruit, chrysanthemum of Peach Seed, root of kudzu vine powder, Indian Bread and predetermined weight part
The water of 8 times of weight of flower, cassia seed, the root of Dahurain angelica, dark plum, sea-buckthorn, 4h is kept at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus
The alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering obtains the first filtrate;The water of 3 times of weight of filter residue is added, in temperature 80-90
2.5h is kept at DEG C, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, low temperature powder
Coarse powder is broken into, the fine powder that the follow-up continued powder of anthocyanidin is broken into 0.05-0.2mm is added;
2) by step 1) in fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear,
Wall material monomer is added dropwise while stirring and carries out wall building, nano level particulate is formed;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through
Heat exchanger is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 2
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 100g
Probiotics powder agent:20g, wherein containing 6 parts of Bifidobacterium, 6 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders
3 parts of 3 parts of bacillus, 2.6 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:10g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin 4
Part, 2.6 parts of rose, 2.2 parts of Cranberry, 1.3 parts of matrimony vine, 1 part of peach kernel, 1.4 mulberries part, 1 part of chrysanthemum, 1.6 parts of cassia seed, Fu
0.9 part of 1.6 parts of Siberian cocklebur, 1.8 parts of the root of kudzu vine, 1.1 parts of the root of Dahurain angelica, 1.2 parts of dark plum and sea-buckthorn.
Preparation method:
1) preparation of plant extracts:
The peach kernel, Poria cocos and the root of kudzu vine of the parts by weight are crushed respectively, 60 mesh sieves are crossed, ox Peach Seed, kudzu-vine root powder is made
End and Indian Bread;
By the rose, Cranberry, matrimony vine, mulberry fruit, chrysanthemum of Peach Seed, root of kudzu vine powder, Indian Bread and predetermined weight part
The water of 8 times of weight of flower, cassia seed, the root of Dahurain angelica, dark plum, sea-buckthorn, 4h is kept at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus
The alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering obtains the first filtrate;The water of 3 times of weight of filter residue is added, in temperature 80-90
2.5h is kept at DEG C, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, low temperature powder
Coarse powder is broken into, the fine powder that the follow-up continued powder of anthocyanidin is broken into 0.05-0.2mm is added;
2) by step 1) in fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear,
Wall material monomer is added dropwise while stirring and carries out wall building, nano level particulate is formed;
3) by the functional oligose and step 2 of predetermined weight number) in particulate uniformly mix, carried out by heat exchanger
Sterilization, condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 3
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 60g
Probiotics powder agent:2g, wherein containing 5.5 parts of Bifidobacterium, 5.6 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
2.6 parts of 2.5 parts of lactobacillus, 2 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:0.2g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin 1
Part, 0.8 part of rose, 1 part of Cranberry, 0.2 part of matrimony vine, 0.1 part of peach kernel, 0.3 mulberries part, 0.1 part of chrysanthemum, 0.3 part of cassia seed,
0.2 part of 0.3 part of Poria cocos, 0.4 part of the root of kudzu vine, 0.1 part of the root of Dahurain angelica, 0.3 part of dark plum and sea-buckthorn;
Resistant dextrin:200g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 4
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 220g
Probiotics powder agent:50g, wherein in per g probiotic powders containing 5.5 parts of Bifidobacterium, 2.4 parts of lactobacillus bulgaricus,
2.4 parts of 1.7 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:100g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin
6.3 parts, 4.2 parts of rose, 5 parts of Cranberry, 2 parts of matrimony vine, 0.1 part of peach kernel, 2 mulberries part, 1.6 parts of chrysanthemum, 2.5 parts of cassia seed,
1.4 parts of 2.5 parts of Poria cocos, 2.6 parts of the root of kudzu vine, 2 parts of the root of Dahurain angelica, 2 parts of dark plum and sea-buckthorn.
Preparation method:Prepared according to the preparation method of embodiment 2, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 5
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 83g, soy oligosaccharides Icing Sugar 61g, FOS 56g;
Probiotics powder agent:20g, wherein containing 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva in per g probiotic powders
2.2 parts of 0.9 part of lactobacillus, 1.5 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:50g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin
4.5 parts, 3 parts of rose, 4.1 parts of Cranberry, 1.2 parts of matrimony vine, 1.1 parts of peach kernel, 0.8 mulberries part, 1.2 parts of chrysanthemum, cassia seed 1.5
Part, 1.5 parts of Poria cocos, 1.5 parts of the root of kudzu vine, 1.2 parts of the root of Dahurain angelica, 1.2 parts of dark plum and 1 part of sea-buckthorn;
Resistant dextrin:300g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 6
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 43g, mannan-oligosaccharides 40g, FOS 23g, galactooligosaccharide 22g and
Trehalose 19g;
Probiotics powder agent:10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, mouse in per g probiotic powders
2.4 parts of lactobacillus of Lee's sugar, 1.3 parts of Lactobacillus helveticus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and secondary cheese breast
0.9 part of bacillus
Plant extracts composition:4.6g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin
3.9 parts, 4 parts of rose, 1 part of Cranberry, 2 parts of matrimony vine, 1 part of peach kernel, 1.3 mulberries part, 1.3 parts of chrysanthemum, 1 part of cassia seed, Poria cocos 1
Part, 1 part of the root of kudzu vine, 0.8 part of the root of Dahurain angelica, 0.9 part of dark plum and 1.1 parts of sea-buckthorn;
Resistant dextrin 70.5g
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 7
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 80g
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 5 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
2.3 parts of 2.2 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Plant extracts composition:8g, is formed wherein being extracted per g extracts by the raw material of following portions by weight:Anthocyanidin 2.5
Part, 1 part of rose, 1.4 parts of Cranberry.
Preparation method:
1) preparation of plant extracts extract:
By rose, the water of 8 times of weight of Cranberry, 4h is kept at 80-90 DEG C of temperature, 40 DEG C is then cooled to, plus 2
Times weight concentration is 75% alcohol reflux 4h, and filtering obtains the first filtrate;The water of 3 times of weight of filter residue is added, in temperature 80-90
2.5h is kept at DEG C, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, low temperature powder
Coarse powder is broken into, the fine powder that the follow-up continued powder of anthocyanidin is broken into 0.05-0.2mm is added;
2) by step 1) in fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear,
Wall material monomer is added dropwise while stirring and carries out wall building, nano level particulate is formed;
3) by the functional oligose and step 2 of predetermined weight number) in particulate uniformly mix, carried out by heat exchanger
Sterilization, condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 8
The pulvis fermentation composition 32g that embodiment 1 is made;Saccharomycete 1g;Acetobacter 1g;Lactic acid bacteria 1g;Coating solution by
Eudragit L 100-5520g, Hydroxypropyl Methylcellulose Phathalate 5g and the appropriate purifying of castor oil 2.5g additions
Water is prepared;
Preparation method:
(1) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The fermentation composition of embodiment 9
The prescription of the fermentation composition of the present embodiment is:
The pulvis fermentation composition 23.1g that embodiment 3 is made;Saccharomycete 3g;Acetobacter 3g;Lactic acid bacteria 5g;Coating solution
It is appropriate pure by Eudragit L 100-5510g, Hydroxypropyl Methylcellulose Phathalate 2g and castor oil 0.2g addition
Change water to prepare;
Preparation method:
(2) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The plant enzyme of embodiment 10
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 2 months, obtains plant enzyme stoste;
Wherein inoculum concentration is 12%;Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid
In bacillus;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g, and clean section is used.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 11
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 2;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:Add plant of fermentation composition of the weight for 60g step a) preparations into step b)
In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip
140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.
Organoleptic parameters:The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant
Gas, mellow in taste, comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 12
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 3;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Wherein, plant material selects passion fruit 120g, citrus 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery
40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.
Organoleptic parameters:The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 13
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 5;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:The fermentation composition for the step a) preparations that weight is 30~60g is added into step b)
Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 14
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, addition 5~
6L pure water, is well mixed, while add in fermentation composition to the ferment bucket that weight is prepared for 20~50g step a),
Capping, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
C) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 4~6d of fermentation, filtering is produced
Plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 15
The plant enzyme of the present embodiment is prepared from by following methods:
I the fermentation composition of pellet) is prepared according to embodiment 8
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
Observation to the pellet fermentation composition fermentation process of embodiment 8:Pellet fermentation composition during the fermentation, ball
The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten
Solution.
The fermentation composition of comparative examples 1
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Trehalose 80g
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 5 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
2.3 parts of 2.2 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Resistant dextrin 80g.
Preparation method:
1) functional oligose of predetermined weight number, resistant dextrin are uniformly mixed, sterilized by heat exchanger, bar
Part is 99 DEG C, 300s;
Mixture is cooled to 40-45 DEG C after sterilization, and the probiotics for adding scheduled volume is freeze-dried, and dry method is added after being well mixed
Comminutor is granulated, and the fermentation composition of powdery, sterile pack is made.
2) preparation of plant enzyme:
A) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
B) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 2 months, obtains plant enzyme stoste;
Wherein inoculum concentration is 12%;Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid
In bacillus;
C) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Add 30g step 1) prepare fermentation composition, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
The plant enzyme of comparative examples 2
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
B) mixing and initial fermentation:It is inoculated with plant extraction liquid of the compound probiotic into step b), mixing is placed in closed
Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
C) zymotic fluid carries out after-ripening 7d in closed environment, takes Partial fermentation liquid to filter, does organoleptic parameters;Continue after-ripening
14d, takes Partial fermentation liquid to filter, does organoleptic parameters.
Wherein, compound probiotic be Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei,
Lactobacillus acidophilus and Lactobacillus plantarum;Inoculum concentration is 8% (mass percent);
Plant material selects apple 500g, soybean 200g and tomato 300g.
The organoleptic parameters of zymotic fluid after 7 days:The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has
Stimulation, sweet taste are heavier;The organoleptic parameters of zymotic fluid after 14 days:The plant enzyme uniform color, in bronzing, there is strong
Fruity is not stimulated with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method
The fermentation time that ferment needs is longer than the fermentation time of plant enzyme of the present invention.
The fermentation composition of comparative examples 3
The prescription of the fermentation composition of the present embodiment is same as Example 1
Fermentation composition preparation method:
(1) according to the step 1 of embodiment 1) prepare extract:
(2) functional oligose of predetermined weight number, probiotics powder agent, resistant dextrin are uniformly mixed, by mixed powder
Dry granulating machine granulation is added, the granule of fermentation composition, pack, every bag of 30g is made.
Plant enzyme preparation method:Method according to embodiment 10 is prepared into plant enzyme.
Organoleptic parameters:The plant enzyme color and luster is uneven, muddy, has floating object, in bronzing, fruit flavor is thin, and entrance is not
Stimulate.
The viable bacteria survival detection of the fermentation composition of test example 1
Fermentation composition prepared by the embodiment of the present invention 1, embodiment 8 and comparative examples 3, is prepared with sterilized water and survived
Bacterium number is 2 × 1010CFU/mL solution to be measured, is saved backup in 4 DEG C;
The 10mL kept the solution to be measured of going bail for is injected into test tube 1, and 10 are diluted to step by step using ten times-8, take 1mL to dilute
The MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured on flat board (sterilizing), shaken up rapidly on flat board by liquid.Again will
The test tube 2 of solution to be measured equipped with 10mL, which is placed in 80-90 DEG C of water-bath, heats 15-25min, takes the solution to be measured after heating to enter
Row ten is diluted to 10 step by step again-8, 1mL dilutions are taken on flat board, and the MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured into
Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates
Number of viable before and after heating.
As a result show, Viable detection difference 91%, 91.5% and 70.1%.
The viable bacteria survival detection of the plant enzyme of test example 2
1st, simulate the gastric juice and the resistance test of intestinal juice
100g/L hydrochloric acid 16.4mL plus distilled water diluting is taken, it is respectively 1.5,2.5 and 3.5 to make pH value, take the dilute salt of 100mL
Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains simulate the gastric juice, and miillpore filter degerming (0.22 μm) is standby.Take
Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value is adjusted to 6.8 with 0.1moL/L sodium hydroxide solutions;Separately take tryptose
Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, miillpore filter is degerming
(0.22 μm) is standby.
Embodiment 10 that 1mL is kept and the plant enzyme solution of embodiment 15 is taken to be added in 9mL simulate the gastric juice (i.e. ten
Dilute step by step again), and rapid fully mixing on the oscillator, it is subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively 1h, 2h,
Nutrient solution is taken out when 3h, 4h and remaining viable count is counted immediately, is compared, as a result shows with former viable count, viable bacteria is deposited
Motility rate is respectively 98.6% and 99%.Then each 1mL of nutrient solution that different time is digested in simulated gastric fluid is taken, is inoculated in respectively
9mL pH value is sampled in 0,3,6,24h respectively in 6.8 simulated intestinal fluid, to be placed in 30-45 DEG C of quiescent culture 2-4h, is determined
Its viable count, is compared with former viable count, and it is 99% as a result to show Viable detection.
The experiment of the nti-freckle of the plant enzyme of test example 3
1 tested crowd
18-65 Sui subject of age 120, is that face has chloasma, is filbert to dark brown, the spot of boundary clear
Piece, no inflammatory performance and the scales of skin that peel off;Without obvious subjective symptoms;Without obvious endocrine system disease, and exclude pigment caused by other diseases
It is calm.
2 packets and test-meal
Test-meal group 96 and control group 24 are randomly divided into by the color of the spot of subject, area situation;Specific test-meal group
For:
Group 1:The plant enzyme of embodiment 10;
Group 2:The plant enzyme of embodiment 14;
Group 3:The plant enzyme of embodiment 15;
Group 4:The plant enzyme of comparative examples 1;
By 30ml/ times, twice a day, warm water dilution is edible, continuous edible 30 days.Control group is blank control.It is tested
Person stops using other oral and external applications about the articles for use of face-beautifying and spot-removing during testing.Original diet is not changed during experiment
Custom, normal diet.
3 efficiency indexes
3.1 facial area spot sizes are detected:With the area (mm of tape measure whole facial area spot before and after tested2);
3.2 facial area spot shades are detected:According to Institute of Geography, Academia Sinica's development and design, Mapping Press
Publish within 1992《Use standard color card》Brown (Y+M+BK) colour atla in (first edition) is the judge index of the chloasma depth:
I degree (15,20,5), II degree (30,40,10) III degree (40,60,15);1 point, 2 points, 3 points is counted respectively.
4 effect criterions
Effectively:Chloasma color declines I degree, and area, which is reduced, is more than 10%, and does not produce new chloasma;It is invalid:Chloasma
Color and area are without significant change.
5 results
Subject's facial area spot size situation is shown in Table 1;
The change of the spot size and shade of the subject's chloasma of table 1
As shown in Table 1, group 1-3 examination trencherman area of chloasma, which is reduced, is more than 10%, and color integration is decreased obviously, itself
Front and rear to compare and compared with control group, difference has conspicuousness, i.e. plant enzyme of the invention to have expelling toxin and dispelling freckle effect.Effect
Group 3 is evident as the most, secondly group 1, group 2;Group 4 is compared with other groups, and effect is worst, it is seen that the plant of plant enzyme of the invention
Extract can strengthen expelling toxin and dispelling freckle effect.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that:Not
In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The scope of invention is limited by claim and its equivalent.
Claims (10)
1. a kind of prepare the fermentation composition with the plant enzyme nursed one's health and strengthen human detoxification moistening face, its feature exists
In the fermentation composition is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight:
0.01~10 part of 3~22 parts of functional oligose, 0.1~5 part of probiotics and plant extracts;
The plant extracts is mainly prepared from by the raw material of following parts by weight:10~63 parts of anthocyanidin, 8~42 parts of rose
Rare flower and 10~50 parts of Cranberry;
The preparation method of the fermentation composition at least comprises the steps:
The fine powder that the functional oligose and plant extracts of predetermined weight number are made uniformly mixes sterilization, with benefit after sterilization
Raw bacterium powder is well mixed, and the fermentation composition of powdery is made in granulation.
2. fermentation composition as claimed in claim 1, it is characterised in that the plant extracts also includes following weight parts
Raw material is extracted and formed:2~20 parts of matrimony vine, 1~20 part of peach kernel, 3~20 parts of mulberries, 1~16 part of chrysanthemum, 3~25 parts
Cassia seed, 3~25 parts of Poria cocos, 4~26 parts of the root of kudzu vine, 1~20 part of the root of Dahurain angelica, 3~20 parts of dark plum and 2~14 parts
Sea-buckthorn.
3. fermentation composition as claimed in claim 2, it is characterised in that the fermentation composition is 5~10 by parts by weight
The functional oligose, 1~3 part of probiotics and 0.5~1 part of plant extracts composition of part;
The plant extracts is extracted by the raw material of following parts by weight and formed:25~40 parts of anthocyanidin, 10~26 parts of rose
Flower, 14~22 parts of Cranberry, 6~13 parts of matrimony vine, 5~10 parts of peach kernel, 6~14 parts of mulberries, 5~10 parts of chrysanthemum, 9
~16 parts of cassia seed, 9~16 parts of Poria cocos, 12~18 parts of the root of kudzu vine, 6~11 parts of the root of Dahurain angelica, 6~12 parts of dark plum and 5
~9 parts of sea-buckthorn.
4. fermentation composition as claimed in claim 1, it is characterised in that it is 10 that the fermentation composition, which also includes parts by weight,
~30 parts of resistant dextrin.
5. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is trehalose;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:45~60 parts of Bifidobacterium,
50~60 parts of Lactobacillus rhamnosus, 22~30 parts of Lactobacillus casei, 18~26 parts of lactobacillus acidophilus and Lactobacillus plantarum 23~
30 parts, the viable bacteria content of the probiotics powder agent is 2 × 106~2 × 1010cfu/g。
6. the fermentation composition as any one of claim 1-5, it is characterised in that the fermentation composition also includes weight
Measure saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria that number is 1~3 part;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight laggard
Row extrusion makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight, made
It is standby into softwood thing;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce pellet
Fermentation composition;
Wherein, the coating solution is by the adjacent benzene for 100-55,0.1-2 parts of the Eudragit L that parts by weight are 0.4-10 parts
Dioctyl phthalate hydroxypropyl methyl cellulose and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step
Suddenly:
1) preparation of plant extracts:
The peach kernel, Poria cocos and the root of kudzu vine of the parts by weight are crushed respectively, 60 mesh sieves are crossed, be made ox Peach Seed, root of kudzu vine powder and
Indian Bread;
By Peach Seed, root of kudzu vine powder, the rose of Indian Bread and predetermined weight part, Cranberry, matrimony vine, mulberry fruit, chrysanthemum, certainly
The water of 8 times of weight of pine torch, the root of Dahurain angelica, dark plum, sea-buckthorn, keeps 4h at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus 2 times of weights
The alcohol reflux 4h that concentration is 75% is measured, filtering obtains the first filtrate;The water of 3 times of weight of filter residue is added, at 80-90 DEG C of temperature
Keep 2.5h, be then cooled to 30 DEG C, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, low-temperature grinding into
Coarse powder, adds the fine powder that the follow-up continued powder of anthocyanidin is broken into 0.05-0.2mm;
2) by step 1) in fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear, while stirring
Mix side and the progress wall building of wall material monomer is added dropwise, form nano level particulate;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through heat exchange
Device is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, and adding dry method after being well mixed makes
Grain machine granulation, is made the fermentation composition of powdery, sterile packing is produced.
8. there is the system for the plant enzyme nursed one's health and strengthen human detoxification moistening face any one of a kind of claim 1-5
Preparation Method, it is characterised in that the described method comprises the following steps:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5~6L
Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope
Lid, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering produces plant
Thing ferment.
9. there is the system for the plant enzyme nursed one's health and strengthen human detoxification moistening face any one of a kind of claim 1-5
Preparation Method, it is characterised in that the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carries out lucifuge
Fermentation 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, gram
It is several in lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with
And plant material 0.5kg, add 150ml plant enzyme stoste fermented in closed environment, fermentation temperature be 28~30
DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is constant, after
Supervention ferment 3-4d, filtering, produces plant enzyme.
10. there is the preparation method for the plant enzyme nursed one's health and strengthen human detoxification moistening face described in a kind of claim 6, its
It is characterised by, the described method comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water
And plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, sent out in closed environment
Ferment;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, and ventilate 2-4 daily
It is secondary, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
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