CN107048409A

CN107048409A - Prepare the fermentation composition and preparation method of the plant enzyme with conditioning insomnia and dreamful sleep improvement sleep effect

Info

Publication number: CN107048409A
Application number: CN201710233339.XA
Authority: CN
Inventors: 姜红成
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-04-11
Filing date: 2017-04-11
Publication date: 2017-08-18

Abstract

The invention provides the fermentation composition for preparing the plant enzyme for improving sleep effect with conditioning insomnia and dreamful sleep, it is made up of functional oligose, probiotics and Chinese medicine；The Chinese medical extract is extracted by the raw material of following parts by weight and formed：9~33 parts of lily, 10~38 parts of spina date seed, 3~20 parts of Poria cocos, 3~22 parts of sealwort, 2~30 parts of matrimony vine, 3~15 parts of lotus nut and 5~30 parts of mulberries.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", the industrialized production and family manufacture of suitable all kinds of plant enzymes.

Description

Prepare the fementative composition for the plant enzyme for improving sleep effect with conditioning insomnia and dreamful sleep Thing and preparation method

Technical field

The invention belongs to prevent conditioning maintenance product field, more particularly to prepared by one kind to improve sleep with conditioning insomnia and dreamful sleep The fermentation composition and preparation method of the plant enzyme of effect.

Background technology

Plant enzyme is always the best seller of ferment class product.Plant enzyme is by tens of kinds of different vegetables and fruits, cereal, seas The plant such as algae and mushroom class, develops through spontaneous fermentation, remains the nutrition elite in plant, contains the various of human body needs Ferment, oligosaccharides and a variety of vitamins and mineral matter, can produce abundant SOD antioxidase compositions again, improve anti-oxidant energy in vivo Power, and then strengthen immunity.

Existing traditional handicraft and modern crafts to ferment has all done further improvement, while also occurring in that some addition benefits Health food prepared by the ferment and ferment of raw bacterium.Probiotics fermention fruits and vegetables, can activate the effective efficiency composition in fruits and vegetables, molten Go out active effect material of more elite.But the fermentate of existing ferment can not be used flexibly on a large scale, and can not be in different ferment It is convenient in the industrial manufacturing processes of element to use；In order to improve ferment in terms of metabolism, coordinating intestines and stomach function in vivo is promoted Effect, and adapt to use on a large scale, applicant further improves to the fermentate for preparing ferment, and develops raising ferment The formula of the function affect of element, as crowd's symptom for insomnia and dreamful sleep carries out developing the ferment with improvement effect.Have one A little Chinese prescriptions for being related to improvement sleep, a kind of ferment rhizoma Gastrodiae health drinks for improving sleep of its Patent CN106244372A Product and preparation technology and application, using fresh rhizoma Gastrodiae as primary raw material, and are equipped with fresh rose, myotonin, wheat, spina date seed, mulberries, Chinese holly Qi is auxiliary material, is formed using orientation ferment chemical industry skill fermentation, traditional wine-making technology is combined with modern ferment zymotechnique, Using functional component gastrodin content as index directed screening distiller's yeast and enzyme class, by the effect of microorganism and enzyme by raw material Functional component is extracted, then obtains product after aseptic filtration membrane filtration.But the preparation of above-mentioned formula is only adapted to industrialized production, And fixed plant enzyme stoste can not meet different taste Man's Demands when reusing, and its preparation method is coarse, is prepared into Ferment effervescent tablet in Chinese medicine dreg sense it is strong, mouthfeel is not good.

There is physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degraded in addition, functional oligose is a class, do not inhaled in small intestine Receive, reach the oligosaccharide of big intestines；Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, and with promoting, human body is prebiotic The physiological functions such as the propagation of bacterium.By the way that probiotics and functional oligose are used in conjunction with, between the two with synergy, energy Promote the regulatory function to gut flora.Co between probiotics and oligosaccharide, be probiotic in symphysis unit and Co between prebiotics, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria Growth；Research shows that both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no This effect.

On the other hand, existing ferment bucket, prepares plant enzyme so that plant enzyme using fruit, sugar and water as raw material It can be prepared in family, it is easy to operate.But also having many weak points, the time prepared by 1. ferment is still longer, Er Qierong Easily prepare failure；2. made from plant enzyme effect it is indefinite, user can not oneself control prepare effect of plant enzyme, than Such as there is conditioning insomnia and dreamful sleep, improve the plant enzyme of sleep quality；3. the low molecular sugar and polysaccharide in the plants such as fruit are most It can not utilize, it is impossible to be converted into the oligosaccharide of given efficacy.For the deficiency, applicant, which puts forth effort research, has shortening ferment system Standby time, fermentation composition easy to use and that there is improvement sleep quality effect, i.e. ferment bag.

The content of the invention

It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention It is to propose a kind of fermentation composition for the plant enzyme for preparing and improving sleep effect with conditioning insomnia and dreamful sleep, is direct putting type hair Probiotics and functional oligose are used in combination yeast-like fungi agent, the fermentation composition, while adding traditional Chinese medicine ingredients, make said composition While with good gut flora regulatory function, the conversion of small molecular sugar and polysaccharide to oligosaccharide can be promoted.The hair Plant enzyme prepared by ferment composition, which plays conditioning insomnia and dreamful sleep, improves effect of sleep.

Technical scheme is as follows：

It is a kind of to prepare the fermentation composition that conditioning insomnia and dreamful sleep improves the plant enzyme of sleep effect, it is direct putting type plant ferment Plain fermenting agent, the fermentation composition includes the component of following parts by weight：3~22 parts of functional oligose, probiotics 0.1~ 5 parts and 0.01~10 part of plant extracts,

Wherein, the probiotics is the one or more in Bifidobacterium, lactobacillus and streptococcus thermophilus Freeze-dried powder；

The Chinese medical extract is extracted by the raw material of following parts by weight and formed：9~33 parts of lily, 10~38 parts of wild jujube Benevolence, 3~20 parts of Poria cocos, 3~22 parts of sealwort, 2~30 parts of matrimony vine, 3~15 parts of lotus nut and 5~30 parts of mulberry Shen；

The preparation method of the fermentation composition at least comprises the steps：

The fine powder that the functional oligose and Chinese medical extract of predetermined weight number are made uniformly mixes sterilization, after sterilization It is well mixed, granulates with probiotic powder, the fermentation composition of powdery is made.

Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity；And the traditional Chinese medicine ingredients added, enter One step realizes conditioning insomnia and dreamful sleep, improves effect of sleep quality.And powdery, the life of implementation phase formula is made in fermentation composition Production, be easy to the later stage direct plunge into use or family oriented use.

Further, functional oligose of the fermentation composition by parts by weight for 5~10 parts, 1~3 part prebiotic Bacterium and 0.5~1 part of Chinese medical extract composition.

Further, Chinese medical extract is extracted by the raw material of following parts by weight and formed：15~20 parts of lily, 16~25 parts Spina date seed, 10~15 parts of Poria cocos, 10~16 parts of sealwort, 9~16 parts of matrimony vine, 5~9 parts of lotus nut and 10~15 The mulberries of part.

In such scheme, the functional oligose is selected from chitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew Oligosaccharide, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, One or more in trehalose, palatinose and inulin；

Bifidobacterium includes bifidobacterium longum (B.longum), short Bifidobacterium Bifidum (B.breve) (B.breve), bifidobacterium infantis And the one or more in bifidobacterium lactis (B.ifidobacterium) (B.infantis)；

Lactobacillus includes Lactobacillus rhamnosus (L.rhamnosus), Lactobacillus casei (Lactobacillus Casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus plantarum), the newborn bar of saliva Bacterium (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus helveticus), Bu Shi lactobacillus (Lactobacillus buchneri), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), the newborn bar of secondary cheese One kind or several in bacterium (Lactobacillus paracasei) and lactobacillus reuteri (Lactobacillus reuteri) Kind.

The preferred scheme of the fermentation composition of the present invention, in addition to the resistant dextrin that parts by weight are 10~30 parts, resistance Dextrin is used in conjunction with other components, further promotes intestines peristalsis, keeps intestinal health, and control the absorption of grease and sugar.

Further, the functional oligose is palatinose；

The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：Bifidobacterium 4.5~ 6 parts, 1~2.6 part of Lactobacillus rhamnosus, 2~2.8 parts of Lactobacillus casei, 2.3~3 parts of lactobacillus acidophilus and Lactobacillus plantarum 2.3~3 parts, the viable bacteria content of the probiotics powder agent is 2 × 10⁶~2 × 10¹⁰cfu/g.By all kinds of strains of reasonable disposition and Consumption, optimizes the strain of this patent products'texture, can effectively shorten fermentation composition in the time for preparing plant enzyme, and can make It prepares the stable plant enzyme of texture.

The source of above-mentioned all kinds of probiotics powder agents：It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of Strain is collected by centrifugation with after washing thalline, drying is prepared into active powder through Liquid Culture.

In one of them preferred scheme, the fermentation composition also include parts by weight for 1~3 part saccharomycete, 1~ 3 parts of acetobacter and 1~5 part of lactic acid bacteria；

The preparation method of the fermentation composition also includes：

(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight Extrusion is carried out afterwards and makes ball, and capsule core is made；

(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the parts by weight It is even, softwood thing is made, it is standby；

(3) obtained capsule core is placed in the coating solution prepared in advance, be coated；

(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce Pellet fermentation composition；

Wherein, the coating solution is by 100-55,0.1-2 parts of Eudragit L that parts by weight are 0.4-10 parts Hydroxypropyl Methylcellulose Phathalate and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.

It is a further object to provide a kind of preparation method of above-mentioned fermentation composition, methods described includes following Step：

1) preparation of Chinese medical extract：

The Poria cocos and sealwort of the parts by weight are crushed respectively, 60 mesh sieves are crossed, Indian Bread and Solomonseal Rhizome is made；

Lily, spina date seed, matrimony vine, lotus nut and the mulberries of Indian Bread, Solomonseal Rhizome and predetermined weight part are used 10 The water of times weight, keeps 4h at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus the alcohol reflux that 2 times of weight concentrations are 75% 4h, filtering, obtains the first filtrate；The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is into being ground into 0.05-0.2mm Medicine fine powder；

2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed Shearing, is added dropwise wall material monomer and carries out wall building, form nano level Chinese medicine particulate while stirring；

3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in Chinese medicine particulate uniformly mix, Sterilized by heat exchanger, condition is 99 DEG C, 300s；

4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry Method comminutor is granulated, and the fermentation composition of powdery is made, and sterile packing is produced.

Fermentation composition viable bacteria rate prepared by the above method is high, the traditional Chinese medicine fine powder delicate mouthfeel of particulate processing, Chinese medicine particulate The capsule heart is slowly discharged, with good slow release effect by processes such as dissolving, infiltration, diffusions through membranous wall.

A further object of the present invention is to provide a kind of plant enzyme for having and nursing one's health insomnia and dreamful sleep improvement sleep effect, is Formulated for personal or Homemade plant enzyme, the preparation method of the plant enzyme comprises the following steps：

(a) powdery fermentation composition is prepared

(b) mixed material：1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5 ~6L pure water, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket Interior, capping carries out lucifuge fermentation in fermentation temperature is 28~30 DEG C of environment；

(c) ferment：Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering, i.e., Obtain plant enzyme.

This method simple and fast, realizes the self-control of various functions plant enzyme, and this method preferably uses ferment bucket and made Standby, ferment bucket is from market sale；Also the vessel of closed environment can be used to be prepared.

Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps：

A) fermentation composition of powdery is prepared

B) preparation of plant extraction liquid：3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid；

C) preparation of plant enzyme stoste：Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out Lucifuge is fermented 1~2 month, obtains plant enzyme stoste；

Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter；

D) preparation of plant enzyme：From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；Fermentation composition prepared by 30g step a) is added, fermentation temperature is not Become, continue the 3-4d that ferments, filtering produces plant enzyme.

Which is applied to industrial production, with reference to the secondary of plant enzyme stoste first fermentation and fermentation composition of the present invention Multistage deep layer liquid state fermentation is realized in fermentation；Greatly improve fermentation efficiency.It can release in fermentation process and be enclosed in Chinese medicine or plant Structure and intracellular nutrients element, retain the original active material of plant, insoluble fibre is changed into solvable in plant Property dietary fiber improve effect of original plant.

The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition also constructed for double-deck pellet Method, the preparation method comprises the following steps：

I the fermentation composition of pellet) is prepared

II) the preparation of plant extraction liquid：3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and 10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid；

III) the preparation of plant enzyme：From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out Fermentation；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；Ferment after 1~2d, insertion conduit is passed through air, daily ventilation 2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C；Filtered after 5~7d of fermentation, produce plant enzyme.

This method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation Realize second order fermentation, or even multistage fermentation；The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries Its high effect.

The present invention has the following advantages that compared with prior art：

The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly with the bacterium bag of pulvis in city Sold on face, can also put into the industrialized production of all kinds of plant enzymes, realize matching somebody with somebody i.e. with the work(with diversification for plant enzyme Energy property configuration, is greatly enhanced the preparation efficiency of plant enzyme.

The traditional Chinese medicinal components added in the fermentation composition of the present invention have its physiologically active and health-care effect, while being prebiotic Bacterium supplements the nutrients in fermentation, and the traditional Chinese medicine ingredients with higher practical value, and the present invention can effectively nurse one's health insomnia and dreamful sleep Symptom, improves the sleep quality of insomnia crowd；Compatibility rationally, is treated between probiotics, functional oligose and Chinese medicine each component Effect is clear and definite, the effect with Synergistic.

The plant enzyme of the present invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body The comprehensive plant enzyme of benefit, metabolism and function adjustment of these enzymes to human body plays an important role, while also containing vitamin, mineral The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group Traditional Chinese medicine ingredients in compound are changed through probiotics, are become small molecule by macromolecular, are easily absorbed by the body；The addition of probiotics can Toxicity can be decomposed by improving in Chinese medicine taste, fermentation process, realization really has no toxic side effect, while prepared by the fermentation composition Plant enzyme improves drug effect compared to traditional Chinese medicine.Suitably the crowd of having a sleepless night uses the plant ferment, is taken as daily drinks, Improve lipid-metabolism from the angle effected a permanent cure, improve body immunity, improve sleep quality etc., realize the purpose of health care.

In addition, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.

The source of the Chinese medicine material composition of the present invention：

Lily：This product is liliaceous plant tiger lily Lilium lancifolium Thunb., lily Lilium brownii F.E.Brown var.viridulum Baker or Lilium tenuifolium Lilium pumilum DC. dry meat scale leaf.

Spina date seed：This product is rhamnaceae plant wild jujube Ziziphus jujuba Mill.var.spinosa (Bunge) Hu Ex H.F.Chou dry mature seed.

Poria cocos：This product is On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf dry sclerotia.

Sealwort：This product is liliaceous plant P. kingianum Polygonatum kingianum Coll.et Hemsl., sealwort Polygonatum sibiricum Red. or David's-harp Polygonatum cyrtonema Hua dry rhizome.

Matrimony vine：For matrimony vine of solanaceae plant or lycium barbarum.

Lotus nut：This product is the green in the middle of nymphaeaceae plant lotus Nelumbo nucifera Gaertn. mature seed Radicle (lotus nut).

Mulberries：This product is moraceae plants mulberry Morus alba L. drying fruit ear.

Embodiment

The fermentation composition of embodiment 1

The prescription of the fermentation composition of the present embodiment is：

Functional oligose：Palatinose 80g；

Probiotics powder agent：24g, wherein containing 4.5 parts of Bifidobacterium, 1 part of Lactobacillus rhamnosus, cheese in per g probiotic powders 2.3 parts of 2 parts of lactobacillus, 2.3 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Traditional Chinese medicine ingredients：8g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight：1.5 parts of lily, 1 part of 1.6 parts of spina date seed, 1 part of Poria cocos, 1 part of sealwort, 0.9 part of matrimony vine, 0.5 part of lotus nut and mulberries；

Resistant dextrin 80g.

Preparation method：

(1) preparation of Chinese medical extract：

By lily, spina date seed, matrimony vine, lotus nut and the mulberries of Indian Bread, Solomonseal Rhizome and predetermined weight part with 8 times The water of weight, 4h is kept at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus the alcohol reflux that 2 times of weight concentrations are 75% 4h, filtering, obtains the first filtrate；The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is into being ground into 0.05-0.2mm Medicine fine powder；

(2) height that 3000-5000 turns is carried out after the traditional Chinese medicine fine powder in step (1) is mixed with emulsifying agent, water with cutter Speed shearing, is added dropwise wall material monomer and carries out wall building, form nano level Chinese medicine particulate while stirring；

(3) the Chinese medicine particulate in the functional oligose, resistant dextrin and step (2) of predetermined weight number is uniformly mixed Close, sterilized by heat exchanger, condition is 99 DEG C, 300s；

(4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed Dry granulating machine is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.

The fermentation composition of embodiment 2

Functional oligose：Palatinose 50g

Probiotics powder agent：10g, wherein containing 6 parts of Bifidobacterium, 2.6 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders 3 parts of 2.8 parts of lactobacillus, 3 parts of lactobacillus acidophilus and Lactobacillus plantarum；Traditional Chinese medicine ingredients：5g, wherein per g Chinese medical extracts under The raw material extraction for stating parts by weight is formed：2 parts of lily, 2.5 parts of spina date seed, 1.5 parts of Poria cocos, 1.6 parts of sealwort, 1.6 parts of matrimony vine, lotus 1.5 parts of 0.9 part of the sub- heart and mulberries

Preparation method：

(1) preparation of Chinese medical extract：

(3) the Chinese medicine particulate in the functional oligose of predetermined weight number and step (2) is uniformly mixed, passes through heat exchange Device is sterilized, and condition is 99 DEG C, 300s；

The fermentation composition of embodiment 3

Functional oligose：Palatinose 60g

Probiotics powder agent：2g, wherein containing 5 parts of Bifidobacterium, 2.2 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders 2.5 parts of 2.5 parts of bacillus, 2.5 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Traditional Chinese medicine ingredients：0.2g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight：Lily 0.9 0.5 part of part, 1 part of spina date seed, 0.3 part of Poria cocos, 0.3 part of sealwort, 0.2 part of matrimony vine, 0.3 part of lotus nut and mulberries

Resistant dextrin 200g

Preparation method：Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.

The fermentation composition of embodiment 4

Functional oligose：Chitosan 104g, mannan-oligosaccharides 116g；

Probiotics powder agent：50g, wherein containing 5.5 parts of Bifidobacterium, 1.5 parts of Lactobacillus rhamnosus in per g probiotic powders, doing 2.6 parts of 2.3 parts of Lactobacillus paracasei, 2.6 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Traditional Chinese medicine ingredients：100g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight：Lily 3.3 3 parts of part, 3.8 parts of spina date seed, 2 parts of Poria cocos, 2.2 parts of sealwort, 3 parts of matrimony vine, 1.5 parts of lotus nut and mulberries

Preparation method：Prepared according to the preparation method of embodiment 2, pulvis, every bag of 30g is made.

The fermentation composition of embodiment 5

Functional oligose：Xylo-oligosaccharide 83g, soy oligosaccharides Icing Sugar 61g, FOS 56g；

Probiotics powder agent：20g, wherein containing 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva in per g probiotic powders 2.2 parts of 0.9 part of lactobacillus, 1.5 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Traditional Chinese medicine ingredients：50g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight：2.2 parts of lily, 1.5 parts of 3 parts of spina date seed, 1.5 parts of Poria cocos, 1.5 parts of sealwort, 1.8 parts of matrimony vine, 1 part of lotus nut and mulberries；

Resistant dextrin 300g

The fermentation composition of embodiment 6

Functional oligose：Xylo-oligosaccharide 43g, mannan-oligosaccharides 40g, FOS 23g, galactooligosaccharide 22g and Trehalose 19g；

Probiotics powder agent：10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, mouse in per g probiotic powders 2.4 parts of lactobacillus of Lee's sugar, 1.3 parts of Lactobacillus helveticus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and secondary cheese breast 0.9 part of bacillus；

Traditional Chinese medicine ingredients：4.55g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight：Lily 1.4 2.2 parts of part, 3.2 parts of spina date seed, 1 part of Poria cocos, 0.6 part of sealwort, 1.7 parts of matrimony vine, 1.2 parts of lotus nut and mulberries；

Resistant dextrin 70.5g

The fermentation composition of embodiment 7

The pulvis fermentation composition 32g that embodiment 1 is made；Saccharomycete 1g；Acetobacter 1g；Lactic acid bacteria 1g；Coating solution by Eudragit L 100-5520g, Hydroxypropyl Methylcellulose Phathalate 5g and the appropriate purifying of castor oil 2.5g additions Water is prepared；

Preparation method：

(1) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight Carry out extrusion and make ball, capsule core is made；

(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight, Softwood thing is made, it is standby；

(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce Pellet fermentation composition, packaging.

Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core；It is preferred that pellet diameters 5mm, capsule core is a diameter of 2.8mm。

The fermentation composition of embodiment 8

The pulvis fermentation composition 23.1g that embodiment 3 is made；Saccharomycete 3g；Acetobacter 3g；Lactic acid bacteria 5g；Coating solution It is appropriate pure by Eudragit L 100-5510g, Hydroxypropyl Methylcellulose Phathalate 2g and castor oil 0.2g addition Change water to prepare；

Preparation method：

(2) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight Carry out extrusion and make ball, capsule core is made；

The plant enzyme of embodiment 9

The plant enzyme of the present embodiment is prepared from by following methods：

A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1；

C) preparation of plant enzyme stoste：Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out Lucifuge is fermented 2 months, obtains plant enzyme stoste；

Wherein inoculum concentration is 12%；Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid In bacillus；

Wherein, plant material selects apple 500g, guava soybean g and tomato 300g, and clean section is used.

Organoleptic parameters：The plant enzyme uniform color, free from admixture, in bronzing, there is strong fruity and fermentation fragrance, mouth Sense is mellow, and comfortable acid, entrance is not stimulated, and pleasant impression is sweet.

The plant enzyme of embodiment 10

A) preparation of fermentation composition：Prepared according to the fermentation composition of embodiment 2；

B) preparation of plant extraction liquid：1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract Liquid；

C) mixing and initial fermentation：Add plant of fermentation composition of the weight for 60g step a) preparations into step b) In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.

Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip 140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.

Organoleptic parameters：The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant Gas, mellow in taste, comfortable acid, entrance is not stimulated, and pleasant impression is sweet.

The plant enzyme of embodiment 11

A) preparation of fermentation composition：Prepared according to the fermentation composition of embodiment 3；

C) preparation of plant enzyme stoste：Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out Lucifuge is fermented 1 month, obtains plant enzyme stoste；

Wherein, plant material selects passion fruit 120g, citrus 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery 40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.

Organoleptic parameters：The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.

The plant enzyme of embodiment 12

A) preparation of fermentation composition：Prepared according to the fermentation composition of embodiment 5；

C) mixing and initial fermentation：The fermentation composition for the step a) preparations that weight is 30~60g is added into step b) Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.

The plant enzyme of embodiment 13

A) preparation of fermentation composition：Prepared according to the fermentation composition of embodiment 1；

B) mixed material：1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, addition 5~ 6L pure water, is well mixed, while add in fermentation composition to the ferment bucket that weight is prepared for 20~50g step a), Capping, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment；

C) ferment：Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 4~6d of fermentation, filtering is produced Plant enzyme.

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

The plant enzyme of embodiment 14

I the fermentation composition of pellet) is prepared according to embodiment 7

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

Observation to the pellet fermentation composition fermentation process of embodiment 7：Pellet fermentation composition during the fermentation, ball The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten Solution.

The fermentation composition of comparative examples 1

The prescription of the fermentation composition of the present embodiment is：：

Functional oligose：Palatinose 80g；

Resistant dextrin 80g.

Preparation method：

(1) functional oligose of predetermined weight number, resistant dextrin are uniformly mixed, sterilized by heat exchanger, Condition is 99 DEG C, 300s；

(2) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed Dry granulating machine is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.

The plant enzyme of comparative examples 2

A) preparation of fermentation composition：Prepared by the fermentation composition of comparative examples 1；

B) preparation of plant extraction liquid：3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material Enter 10L pure water, be well mixed, stand after extraction 2d, carry out high-temperature sterilization, filtering obtains plant extraction liquid；

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

The plant enzyme of comparative examples 3

A) preparation of plant extraction liquid：1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract Liquid；

B) mixing and initial fermentation：It is inoculated with plant extraction liquid of the compound probiotic into step b), mixing is placed in closed Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

C) zymotic fluid carries out after-ripening 7d in closed environment, takes Partial fermentation liquid to filter, does organoleptic parameters；Continue after-ripening 14d, takes Partial fermentation liquid to filter, does organoleptic parameters.

Wherein, compound probiotic is Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus and plant Lactobacillus；Inoculum concentration is 8% (mass percent)；

Plant material selects apple 500g, soybean 200g and tomato 300g.

The organoleptic parameters of zymotic fluid after 7 days：The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has Stimulation, sweet taste are heavier；The organoleptic parameters of zymotic fluid after 14 days：The plant enzyme uniform color, in bronzing, there is strong Fruity is not stimulated with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method The fermentation time that ferment needs is longer than the fermentation time of embodiment of the present invention plant enzyme.

Comparative examples 4

The prescription of the fermentation composition of the present embodiment is same as Example 1

Fermentation composition preparation method：

(1) preparation of Chinese medical extract：

(2) functional oligose of predetermined weight number, probiotics powder agent, resistant dextrin are uniformly mixed, by mixed powder Dry granulating machine granulation is added, the granule of fermentation composition, pack, every bag of 30g is made.

Plant enzyme preparation method：Method according to embodiment 9 is prepared into plant enzyme.

Organoleptic parameters：The plant enzyme color and luster is uneven, muddy, has floating object, in bronzing, fruit flavor is thin, and entrance is not Stimulate

The viable bacteria survival detection of the fermentation composition of test example 1

Fermentation composition prepared by the embodiment of the present invention 1, embodiment 7 and comparative examples 4, is prepared with sterilized water and survived Bacterium number is 2 × 10⁸CFU/mL solution to be measured, is saved backup in 4 DEG C；

The 10mL kept the solution to be measured of going bail for is injected into test tube 1, and 10 are diluted to step by step using ten times^-8, take 1mL to dilute The MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured on flat board (sterilizing), shaken up rapidly on flat board by liquid.Again will The test tube 2 of solution to be measured equipped with 10mL, which is placed in 80-90 DEG C of water-bath, heats 15-25min, takes the solution to be measured after heating to enter Row ten is diluted to 10 step by step again^-8, 1mL dilutions are taken on flat board, and the MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured into Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates Number of viable before and after heating.

As a result show, Viable detection is respectively 89.1%, 90% and 63.2%.

The viable bacteria survival detection of the plant enzyme of test example 2

1st, simulate the gastric juice and the resistance test of intestinal juice

100g/L hydrochloric acid 16.4mL plus distilled water diluting is taken, it is respectively 1.5,2.5 and 3.5 to make pH value, take the dilute salt of 100mL Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains simulate the gastric juice, and miillpore filter degerming (0.22 μm) is standby.Take Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value is adjusted to 6.8 with 0.1moL/L sodium hydroxide solutions；Separately take tryptose Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, miillpore filter is degerming (0.22 μm) is standby.

Embodiment 9 that 1mL is kept and the plant enzyme solution of embodiment 14 is taken to be added in 9mL simulate the gastric juice (i.e. ten Dilute step by step again), and rapid fully mixing on the oscillator, it is subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively 1h, 2h, Nutrient solution is taken out when 3h, 4h and remaining viable count is counted immediately, is compared, as a result shows with former viable count, viable bacteria is deposited Motility rate is respectively 97.3% and 98%.Then each 1mL of nutrient solution that different time is digested in simulated gastric fluid is taken, is inoculated in respectively 9mL pH value is sampled in 0,3,6,24h respectively in 6.8 simulated intestinal fluid, to be placed in 30-45 DEG C of quiescent culture 2-4h, is determined Its viable count, is compared with former viable count, and it is 99% as a result to show Viable detection.

The plant enzyme of test example 3 improves sleep function detection experiment

1st, experimental animal is grouped

Healthy adult is chosen, body weight is close, the male mice of same kind 300, and every 60 mouse are divided into one group, It is divided into five groups, each component is 4 groups, one group is control group, and other four groups are respectively test group.

2nd, trial drug

By reagent human body recommended dose be daily 30ml/60kg.bw the plant enzyme of the embodiment of the present invention 8, mouse etc. Dosage is imitated equivalent to 10 times of human body recommended dose, i.e. 5ml/kg.bw takes administration by gavage to gavage, control group fills distilled water, continuously Test 30 days, specific given the test agent is respectively：

Group 1：The plant enzyme of embodiment 9；

Group 2：The plant enzyme of embodiment 13；

Group 3：The plant enzyme of embodiment 14

Group 4：The plant enzyme of comparative examples 2；

3rd, process of the test：

(1), direct sleep laboratory

Observation mouse is given after the test drug of various dose, and control group gives same volume distilled water, if sleep Phenomenon, was judged to enter sleep, righting reflex recovers to be animal awakening, righting reflex loss with righting reflex loss more than 60 seconds It it is animal sleep time, record control group and various dose group sleep number of animals and the length of one's sleep to this period is recovered.

(2) extension yellow Jackets length of one's sleep experiment

After to last sample 20 minutes, each group animal is through yellow Jackets 35mg/kg BW, injection volume is injected intraperitoneally 0.1mL/10g BW, using the righting reflex loss of mouse as index, can observation tested material extend the yellow Jackets length of one's sleep, Experiment is carried out at night.

(3) yellow Jackets sub-threshold dose hypnosis test

After to last sample 20 minutes, each group animal is through yellow Jackets 30mg/kg BW, injection volume is injected intraperitoneally 0.1mL/10g BW, observe the sleep quality of mouse in 30 minutes, using more than 60 seconds persons of righting reflex loss to fall asleep, record 30 The sleep number of animals of each group in minute, calculates incidence of sleep animals, and experiment is carried out at night.

(4), barbital sodium Sleep Latency Test

Through barbital sodium 200mg/kg BW are injected intraperitoneally, injection volume is each group mouse within 20 minutes after to last sample 0.1mL/10g BW, using righting reflex loss as index, can viewing test medicine shorten barbital sodium Sleep latency.

4th, result of the test

4.1st, influence of the trial drug to mouse weight

Influence of the trial drug of table 1 to mouse weight

The mouse weight of each group is compared with the mouse weight of control group before and after table 1, experiment, and there are no significant for difference (P>0.05), show the sample to the body weight increase of mouse without influence.(2), direct sleep laboratory result

The direct sleep laboratory result of the mouse of table 2

As seen from Table 2, the different plant enzymes of orally administration mouse 30 days, there is righting reflex and disappeared in the no animal of each group Lose, i.e., all animals do not enter into sleep state, point out the test plant ferment there is no direct inducing mouse sleep to act on.

(3) influence of the trial drug to the yellow Jackets length of one's sleep

Table 3 extends the result of the test of the yellow Jackets length of one's sleep

As seen from Table 3, the capsule of orally administration mouse various dose 30 days, the length of one's sleep of each group is than control group Extension, and the difference of group 1-3 and control group has conspicuousness (P<0.05), point out the sample that there is extension yellow Jackets sleep The effect of time.It is not good that group 4 is not added with Chinese medical extract composition effect.

(4) influence of the trial drug to yellow Jackets sub-threshold dose syngignoscism

Table 4 extends the result of the test of yellow Jackets sub-threshold dose syngignoscism

As seen from Table 4, the different plant enzymes of orally administration mouse 30 days, the mouse sleep rate of each group is higher than control group, and Group 1-3 compares with pole significant difference (P<0.01), point out the trial drug to cooperate with the yellow Jackets of sub-threshold dose to urge Dormancy is acted on.

(5) trial drug is on the preclinical influence of barbital sodium hypnosis

The preclinical result of the test of barbital sodium hypnosis of table 5

As seen from Table 5, the different plant enzymes of orally administration mouse 30 days, the Sleep latency of each group is than control group Shorten, and the difference of group 1-3 and negative control group is respectively provided with conspicuousness (P<0.01), point out the sample to have and shorten barbital sodium The effect of Sleep latency.

5th, result judgement：

Mouse stomach 30 days are continuously given with the dosage plant enzyme of the present invention equivalent to human body recommended amounts respectively, to Mice Body Weight acts on without directly sleep without influence, can extend the yellow Jackets length of one's sleep, cooperates with yellow Jackets sub-threshold dose hypnosis Effect, shortens barbital sodium Sleep latency, points out the present invention to have the function of improving sleep.

Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this The scope of invention is limited by claim and its equivalent.

Claims

1. a kind of fermentation composition for preparing the plant enzyme for improving sleep effect with conditioning insomnia and dreamful sleep, it is characterised in that The fermentation composition is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight：Work( 0.01~10 part of 3~22 parts of energy property oligosaccharide, 0.1~5 part of probiotics and plant extracts,

Wherein, the probiotics is one or more of jellies in Bifidobacterium, lactobacillus and streptococcus thermophilus Dry powder；

The Chinese medical extract is extracted by the raw material of following parts by weight and formed：9~33 parts of lily, 10~38 parts of spina date seed, 3 ~20 parts of Poria cocos, 3~22 parts of sealwort, 2~30 parts of matrimony vine, 3~15 parts of lotus nut and 5~30 parts of mulberries；

The fine powder that the functional oligose and Chinese medical extract of predetermined weight number are made uniformly mixes sterilization, with benefit after sterilization Raw bacterium powder is well mixed, and the fermentation composition of powdery is made in granulation.

2. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition is 5~10 by parts by weight The functional oligose, 1~3 part of probiotics and 0.5~1 part of Chinese medical extract composition of part.

3. fermentation composition as claimed in claim 1, it is characterised in that the Chinese medical extract by following parts by weight raw material Extraction is formed：15~20 parts of lily, 16~25 parts of spina date seed, 10~15 parts of Poria cocos, 10~16 parts of sealwort, 9~16 The matrimony vine, 5~9 parts of lotus nut and 10~15 parts of mulberries of part；

The functional oligose is selected from chitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, mannan-oligosaccharides, milk ketose, cotton Seed sugar, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, trehalose, palatinose and One or more in inulin；

The Bifidobacterium includes one kind in bifidobacterium longum, bifidobacterium breve, bifidobacterium infantis and bifidobacterium lactis Or it is several；

The lactobacillus include Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus salivarius, Lactobacillus helveticus, Bu Shi lactobacillus, lactobacillus bulgaricus, lactobacillus paracasei and one kind or several in lactobacillus reuteri Kind.

4. fermentation composition as claimed in claim 1, it is characterised in that it is 10 that the fermentation composition, which also includes parts by weight, ~30 parts of resistant dextrin.

5. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is palatinose；

The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：4.5~6 parts of Bifidobacterium, 1~2.6 part of Lactobacillus rhamnosus, 2~2.8 parts of Lactobacillus casei, 2.3~3 parts of lactobacillus acidophilus and Lactobacillus plantarum 2.3~ 3 parts, the viable bacteria content of the probiotics powder agent is 2 × 10⁶~2 × 10¹⁰cfu/g。

6. such as fermentation composition according to any one of claims 1 to 5, it is 1 that the fermentation composition, which also includes parts by weight, ~3 parts of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria；

The preparation method of the fermentation composition also includes：

(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight laggard Row extrusion makes ball, and capsule core is made；

(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight, made It is standby into softwood thing；

Wherein, the coating solution is by the adjacent benzene for 100-55,0.1-2 parts of the Eudragit L that parts by weight are 0.4-10 parts Dioctyl phthalate hydroxypropyl methyl cellulose and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.

7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step Suddenly：

1) preparation of Chinese medical extract：

By Indian Bread, Solomonseal Rhizome and predetermined weight part lily, spina date seed, matrimony vine, lotus nut and mulberries with 10 times of weights The water of amount, 4h is kept at 80-90 DEG C of temperature, is then cooled to 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, Filtering, obtains the first filtrate；The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, mistake Filter, the second filtrate；Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is thin into the Chinese medicine for being ground into 0.05-0.2mm Powder；

2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear, Wall material monomer is added dropwise while stirring and carries out wall building, nano level Chinese medicine particulate is formed；

3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in Chinese medicine particulate uniformly mix, pass through Heat exchanger is sterilized, and condition is 99 DEG C, 300s；

4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, and adding dry method after being well mixed makes Grain machine granulation, is made the fermentation composition of powdery, sterile packing is produced.

8. there is conditioning insomnia and dreamful sleep to improve the preparation of plant enzyme of sleep effect any one of a kind of claim 1-5 Method, it is characterised in that the described method comprises the following steps：

(a) powdery fermentation composition is prepared

(b) mixed material：1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5~6L Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope Lid, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment；

(c) ferment：Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering produces plant Thing ferment.

9. there is conditioning insomnia and dreamful sleep to improve the preparation of plant enzyme of sleep effect any one of a kind of claim 1-5 Method, it is characterised in that the described method comprises the following steps：

A) fermentation composition of powdery is prepared

B) preparation of plant extraction liquid：3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material 10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid；

C) preparation of plant enzyme stoste：Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carries out lucifuge Fermentation 1~2 month, obtains plant enzyme stoste；

Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, gram It is several in lactobacillus, lactobacillus acidophilus and acetobacter；

D) preparation of plant enzyme：From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with And plant material 0.5kg, add 150ml plant enzyme stoste fermented in closed environment, fermentation temperature be 28~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；Fermentation composition prepared by 30g step a) is added, fermentation temperature is constant, after Supervention ferment 3-4d, filtering, produces plant enzyme.

10. having conditioning insomnia and dreamful sleep to improve the preparation method of plant enzyme of sleep effect described in a kind of claim 6, it is special Levy and be, the described method comprises the following steps：

I the fermentation composition of pellet) is prepared

II) the preparation of plant extraction liquid：3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material 10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid；

III) the preparation of plant enzyme：From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water And plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, sent out in closed environment Ferment；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；Ferment after 1~2d, insertion conduit is passed through air, and ventilate 2-4 daily It is secondary, each 10-30min, and control fermentation temperature to be 35~37 DEG C；Filtered after 5~7d of fermentation, produce plant enzyme.