CN107019211A - Prepare the fermentation composition and preparation method of the plant enzyme with the strong renal function of kidney tonifying - Google Patents
Prepare the fermentation composition and preparation method of the plant enzyme with the strong renal function of kidney tonifying Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of fermentation composition of the plant enzyme with the strong renal function of kidney tonifying, is made up of 0.01~10 part of 3~22 parts of functional oligose, 0.1~5 part of probiotics and integration of drinking and medicinal herbs food materials pulvis;Integration of drinking and medicinal herbs food materials pulvis is prepared from by parts by weight for the raw material of following weight parts:2~25 parts of 1~20 part of Chinese yam, 2~25 parts of longan, 1~16 part of agate card, 1~20 part of jujube, 0.5~12 part of matrimony vine and acerola concentrate powder;Additionally provide the plant enzyme of fermentation composition preparation method and its preparation.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", the industrialized production of suitable all kinds of plant enzymes and family making.
Description
Technical field
It is more particularly to a kind of to prepare the plant ferment with the strong renal function of kidney tonifying the invention belongs to prevent conditioning maintenance product field
The fermentation composition and preparation method of element.
Background technology
Plant enzyme is always the best seller of ferment class product.Plant enzyme is by tens of kinds of different vegetables and fruits, cereal, seas
The plant such as algae and mushroom class, develops through spontaneous fermentation, remains the nutrition elite in plant, contains the various of human body needs
Ferment, oligosaccharides and a variety of vitamins and mineral matter, can produce abundant SOD antioxidase compositions again, improve anti-oxidant energy in vivo
Power, and then strengthen immunity.
Existing traditional handicraft and modern crafts to ferment has all done further improvement, while also occurring in that some addition benefits
Health food prepared by the ferment and ferment of raw bacterium.Probiotics fermention fruits and vegetables, can activate the effective efficiency composition in fruits and vegetables, molten
Go out active effect material of more elite.List is carried out using single culture or composite bacteria more than enzyme beverage or Juice fermented beverage
Step fermentation or more fermentation are formed through lengthy fermentation.Such as patent of invention CN201410266394.5 discloses a kind of health care bacterium
Mushroom vegetables and fruits ferment and preparation method thereof, but fermentation process it is cumbersome, it is necessary to vegetables and fruits fermentation, extract mushroom, prepare mushroom extract solution,
Chinese medical extract is prepared, is then obtained through secondary fermentation, and fermentation period is long.CN201510038241.X discloses a kind of ferment
Kinases drink, three fermentation, fermentation period long the shortcomings of complicated with material composition.CN104013947A is disclosed with anti-
Alopecia and the fresh medicine pectase spray coating liquor of hair regrowth, by the fresh Chinese medicine of traditional zymotic and fruits and vegetables preparation;Traditional
Ferment technique can not be used flexibly on a large scale, or without effect fermentation composition is clever in the industrial manufacturing processes of different ferment
Work is used;In order to improve effect of the ferment in terms of metabolism, coordinating intestines and stomach function in vivo is promoted, and adapt to a wide range of
Use, applicant further improves to the fermentate for preparing ferment, and develops the formula for the function affect for improving ferment.
Functional oligose is that a class has physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degraded, can not in intestinal absorption
Reach the oligosaccharide of big intestines;Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, with the increasing for promoting human body probiotics
The physiological function such as grow.By the way that probiotics and functional oligose are used in conjunction with, between the two with synergy, it can promote pair
The regulatory function of gut flora.Co between probiotics and oligosaccharide, is the probiotic and prebiotics in symphysis unit
Between co, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria growing;Grind
Study carefully and show, both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no this effect.
Such as the enzyme composition that patent CN106174179A is related to, probiotics and functional oligose are used in combination, and in addition
Medicine composition, reaches its given efficacy.But its preparation being formulated is applied to individual and directly taken, and the plant enzyme stoste fixed is again
Different taste Man's Demands can not be met when using, and its preparation method is coarse, Chinese medicine dreg in the ferment effervescent tablet being prepared into
Sense is strong, and mouthfeel is not good.
On the other hand, existing ferment bucket, prepares plant enzyme so that plant enzyme using fruit, sugar and water as raw material
It can be prepared in family, it is easy to operate.But also having many weak points, the time prepared by 1. ferment is still longer, Er Qierong
Easily prepare failure;2. made from plant enzyme effect it is indefinite, user can not oneself control prepare effect of plant enzyme, than
Such as it is directed to kidney deficiency alopecia, the plant enzyme of chilly;3. the low molecular sugar and polysaccharide majority in the plants such as fruit can not be utilized, no
The oligosaccharide of given efficacy can be converted into.For the deficiency, applicant, which puts forth effort research, to be had shortening ferment preparation time, uses
Fermentation composition convenient and with the strong renal function of kidney tonifying, i.e. ferment bag.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention
It is to propose a kind of fermentation composition prepared with the strong kidney of kidney tonifying and hair care and hair growth function, is directly putting type fermented microbial inoculum, the hair
Probiotics and functional oligose are used in combination ferment composition, while adding integration of drinking and medicinal herbs food materials composition, said composition is in tool
While having good gut flora regulatory function, integration of drinking and medicinal herbs food materials can be promoted and prepared small in the raw material of plant enzyme
Molecular saccharides and conversion from polysaccharide to oligosaccharide;In addition, by adding polyunsaturated fatty acid, plant prepared by the fermentation composition
Thing ferment warming and invigorating kidney Yang, the strong kidney of kidney tonifying.
Technical scheme is as follows:
A kind of fermentation composition for preparing the plant enzyme with the strong renal function of kidney tonifying, is direct putting type plant enzyme zymophyte
Agent, the fermentation composition includes the component of following parts by weight:3~22 parts of functional oligose, 0.1~5 part of probiotics and medicine
Eat homologous 0.01~10 part of food materials pulvis;
The integration of drinking and medicinal herbs food materials pulvis is prepared from by parts by weight for the raw material of following weight parts:1~20 part of Chinese yam,
2~25 parts of 2~25 parts of longan, 1~16 part of agate card, 1~20 part of jujube, 0.5~12 part of matrimony vine and acerola concentrate powder;
The preparation method of the fermentation composition at least comprises the steps:
The functional oligose of predetermined weight number and integration of drinking and medicinal herbs food materials pulvis are uniformly mixed into sterilization, with benefit after sterilization
Raw bacterium powder is well mixed, and the fermentation composition of powdery is made in granulation.
In the program, the functional oligose is low selected from chitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew
Glycan, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, sea
One or more in algae sugar, palatinose and inulin;
Probiotics is selected from Bifidobacterium (Bifidobacterium), Lactobacillus rhamnosus (L.rhamnosus), cheese breast
Bacillus (Lactobacillus casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus
Plantarum), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus
Helveticus), Bu Shi lactobacillus (Lactobacillus buchneri), lactobacillus bulgaricus
(Lactobacillus.Bulgaricus), lactobacillus paracasei (Lactobacillus paracasei) and the newborn bars of Luo Yishi
One or more in bacterium (Lactobacillus reuteri).
Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle
The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity.Its integration of drinking and medicinal herbs food materials is then passed through
By beneficial flora ferment after produce out and stimulate circulation, promote digestion, lifting body temperature again can health care kidney health drink.
The acerola concentrate powder that the present invention is used is commercially available prod, is not specifically limited.
Further, the fermentation composition is made up of the component of following parts by weight:5~10 parts of functional oligose, benefit
0.1~5 part of 1~3 part of raw bacterium and integration of drinking and medicinal herbs food materials pulvis.
Further, the integration of drinking and medicinal herbs food materials pulvis is prepared from by parts by weight for the raw material of following weight parts:Mountain
6~15 parts of medicine, 8~18 parts of longan, 5~12 parts of agate card, 6~15 parts of jujube, 3~9 parts of matrimony vine and acerola concentrate powder 8~18
Part..
The preferred scheme of the fermentation composition of the present invention, in addition to the resistant dextrin that parts by weight are 10~30 parts, resistance
Dextrin is used in conjunction with other components, further promotes intestines peristalsis, keeps intestinal health, and control the absorption of grease and sugar.
Further, the functional oligose is stachyose;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:Bifidobacterium 4.5~
6 parts, 1~2.6 part of Lactobacillus rhamnosus, 2~2.8 parts of Lactobacillus casei, 2.3~3 parts of lactobacillus acidophilus and Lactobacillus plantarum
2.3~3 parts, the viable bacteria content of the probiotics powder agent is 2 × 106~2 × 1010cfu/g;By all kinds of strains of reasonable disposition and
Consumption, optimizes the strain of this patent products'texture, can effectively shorten fermentation composition in the time for preparing plant enzyme, and can make
It prepares the stable plant enzyme of texture.
The source of above-mentioned all kinds of probiotics powder agents:It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of
Strain is collected by centrifugation with after washing thalline, drying is prepared into active powder through Liquid Culture.
In scheme of the invention further, the fermentation composition also include parts by weight for 1~3 part saccharomycete, 1~
3 parts of acetobacter and 1~5 part of lactic acid bacteria;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight
Extrusion is carried out afterwards and makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the parts by weight
It is even, softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition;
Wherein, the coating solution is by 100-55,0.1-2 parts of Eudragit L that parts by weight are 0.4-10 parts
Hydroxypropyl Methylcellulose Phathalate and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
It is a further object to provide a kind of preparation method of above-mentioned fermentation composition, methods described includes following
Step:
1) preparation of integration of drinking and medicinal herbs food materials pulvis:
Chinese yam is cleaned, peeling simultaneously segment;Longan removes shell stoning, dries;Dried after Removing Kernel for Date;Weight is weighed after processing
Number Chinese yam, longan and jujube, it is stand-by;
By water, (stand-by Chinese yam, longan and jujube is 1 with water ratio:1.5) stand-by Chinese yam, osmanthus are placed into after boiling
Beater is placed in after circle and jujube, 8~12min to be beaten;Feed liquid after mashing, is preheated to 70~80 DEG C, under 25MPa
It is concentrated in vacuo after matter, homogeneous, is spray-dried, produces fine powder 1;
By the water of 8 times of weight of matrimony vine and Ma Ka of predetermined weight part, 4h is kept at 80-90 DEG C of temperature, is then cooled
To 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering, obtain the first filtrate;The water of 3 times of weight of filter residue is added,
2.5h is kept at 80-90 DEG C of temperature, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, freezes and do
Dry, low-temperature grinding continues to be ground into 0.05-0.2mm fine powder 2 into coarse powder;
Fine powder 1, fine powder 2 and acerola concentrate powder are well mixed, integration of drinking and medicinal herbs food materials pulvis is produced;2) by step 1) in
Integration of drinking and medicinal herbs food materials pulvis carries out the high speed shear that 3000-5000 turns with cutter after being mixed with emulsifying agent, water, drip while stirring
Plus wall material monomer carries out wall building, nano level particulate is formed;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through
Heat exchanger is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery is made, and sterile packing is produced.
A further object of the present invention is to provide a kind of plant enzyme of the strong renal function of kidney tonifying, is to be directed to personal or family certainly
Plant enzyme processed and formulate, the preparation method of the plant enzyme comprises the following steps:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5
~6L pure water, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket
Interior, capping carries out lucifuge fermentation in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering, i.e.,
Obtain plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and this method preferably uses ferment bucket and made
Standby, ferment bucket is from market sale;Also the vessel of closed environment can be used to be prepared.
Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Which is applied to industrial production, with reference to the secondary of plant enzyme stoste first fermentation and fermentation composition of the present invention
Multistage deep layer liquid state fermentation is realized in fermentation;Greatly improve fermentation efficiency.It can release in fermentation process and be enclosed in Chinese medicine or plant
Structure and intracellular nutrients element, retain the original active material of plant, insoluble fibre is changed into solvable in plant
Property dietary fiber improve effect of original plant.
The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition also constructed for double-deck pellet
Method, the preparation method comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation
Realize second order fermentation, or even multistage fermentation;The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries
Its high effect.
The present invention has the following advantages that compared with prior art:
The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing
Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly be existed with the bacterium bag of granule
Sell on the market, can also put into the industrialized production of all kinds of plant enzymes, realize that matching somebody with somebody for plant enzyme is used and diversification
Functional configuration, is greatly enhanced the preparation efficiency of plant enzyme.
The traditional Chinese medicinal components added in the fermentation composition of the present invention have its physiologically active and health-care effect, while being prebiotic
Bacterium supplements the nutrients in fermentation, with higher practical value, and the integration of drinking and medicinal herbs food materials component of the present invention have the strong kidney of kidney tonifying,
Improve effect of alopecia and cold of insufficiency type symptom;Rationally, curative effect is bright for compatibility between probiotics, functional oligose and Chinese medicine each component
Really, the effect with Synergistic.
The plant enzyme of the present invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body
The comprehensive plant enzyme of benefit, metabolism and function adjustment of these enzymes to human body plays an important role, while also containing vitamin, mineral
The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group
Integration of drinking and medicinal herbs food materials composition in compound is changed through probiotics, is become small molecule by macromolecular, is easily absorbed by the body;Probiotics
Addition can improve toxicity can be decomposed in integration of drinking and medicinal herbs food materials taste, fermentation process, realization really has no toxic side effect, simultaneously this
Plant enzyme prepared by fermentation composition improves drug effect compared to traditional integration of drinking and medicinal herbs food materials.The plant enzyme is used to adjust
Body detoxification is managed, it takes as daily drinks, improve lipid-metabolism from the angle effected a permanent cure, realize the purpose of the strong kidney of kidney tonifying.
In addition, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big
Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.
Embodiment
The fermentation composition of embodiment 1
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Stachyose 80g
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 1 part of Lactobacillus rhamnosus, cheese in per g probiotic powders
2.3 parts of 2 parts of lactobacillus, 2.3 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Integration of drinking and medicinal herbs food materials:8g, is formed wherein being extracted per g food materials pulvis by the raw material of following portions by weight:6 parts of Chinese yam,
8 parts of 8 parts of longan, 5 parts of agate card, 6 parts of jujube, 3 parts of matrimony vine and acerola concentrate powder;
Resistant dextrin 80g.
Preparation method:
1) preparation of integration of drinking and medicinal herbs food materials pulvis:
Chinese yam is cleaned, peeling simultaneously segment;Longan removes shell stoning, dries;Dried after Removing Kernel for Date;Weight is weighed after processing
Number Chinese yam, longan and jujube, it is stand-by;
By water, (stand-by Chinese yam, longan and jujube is 1 with water ratio:1.5) stand-by Chinese yam, osmanthus are placed into after boiling
Beater is placed in after circle and jujube, 8~12min to be beaten;Feed liquid after mashing, is preheated to 70~80 DEG C, under 25MPa
It is concentrated in vacuo after matter, homogeneous, is spray-dried, produces fine powder 1;
By the water of 8 times of weight of matrimony vine and Ma Ka of predetermined weight part, 4h is kept at 80-90 DEG C of temperature, is then cooled
To 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering, obtain the first filtrate;The water of 3 times of weight of filter residue is added,
2.5h is kept at 80-90 DEG C of temperature, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, freezes and do
Dry, low-temperature grinding continues to be ground into 0.05-0.2mm fine powder 2 into coarse powder;
Fine powder 1, fine powder 2 and acerola concentrate powder are well mixed, integration of drinking and medicinal herbs food materials pulvis is produced;2) by step 1) in
Integration of drinking and medicinal herbs food materials pulvis carries out the high speed shear that 3000-5000 turns with cutter after being mixed with emulsifying agent, water, drip while stirring
Plus wall material monomer carries out wall building, nano level particulate is formed;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through
Heat exchanger is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 2
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Stachyose 50g
Probiotics powder agent:10g, wherein containing 6 parts of Bifidobacterium, 2.6 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
3 parts of 2.8 parts of lactobacillus, 3 parts of lactobacillus acidophilus and Lactobacillus plantarum;Integration of drinking and medicinal herbs food materials composition:5g, wherein per g medicine baking sodas
Agent is extracted by the raw material of following portions by weight and formed:15 parts of Chinese yam, 18 parts of longan, 12 parts of agate card, 15 parts of jujube, 9 parts of matrimony vine with
And 18 parts of acerola concentrate powder;
Preparation method:
1) preparation of integration of drinking and medicinal herbs food materials pulvis:
Chinese yam is cleaned, peeling simultaneously segment;Longan removes shell stoning, dries;Dried after Removing Kernel for Date;Weight is weighed after processing
Number Chinese yam, longan and jujube, it is stand-by;
By water, (stand-by Chinese yam, longan and jujube is 1 with water ratio:1.5) stand-by Chinese yam, osmanthus are placed into after boiling
Beater is placed in after circle and jujube, 8~12min to be beaten;Feed liquid after mashing, is preheated to 70~80 DEG C, under 25MPa
It is concentrated in vacuo after matter, homogeneous, is spray-dried, produces fine powder 1;
By the water of 8 times of weight of matrimony vine and Ma Ka of predetermined weight part, 4h is kept at 80-90 DEG C of temperature, is then cooled
To 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering, obtain the first filtrate;The water of 3 times of weight of filter residue is added,
2.5h is kept at 80-90 DEG C of temperature, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, freezes and do
Dry, low-temperature grinding continues to be ground into 0.05-0.2mm fine powder 2 into coarse powder;
Fine powder 1, fine powder 2 and acerola concentrate powder are well mixed, integration of drinking and medicinal herbs food materials pulvis is produced;2) by step 1) in
Integration of drinking and medicinal herbs food materials pulvis carries out the high speed shear that 3000-5000 turns with cutter after being mixed with emulsifying agent, water, drip while stirring
Plus wall material monomer carries out wall building, nano level particulate is formed;
3) by the functional oligose and step 2 of predetermined weight number) in particulate uniformly mix, carried out by heat exchanger
Sterilization, condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 3
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Stachyose 60g
Probiotics powder agent:2g, wherein containing 5 parts of Bifidobacterium, 2.2 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders
2.5 parts of 2.5 parts of bacillus, 2.5 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Integration of drinking and medicinal herbs food materials composition:0.2g, is formed wherein being extracted per g medicine baking soda agent by the raw material of following portions by weight:Mountain
2 parts of 1 part of medicine, 2 parts of longan, 1 part of agate card, 1 part of jujube, 0.5 part of matrimony vine and acerola concentrate powder;
Resistant dextrin 200g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 4
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Chitosan 104g, mannan-oligosaccharides 116g;
Probiotics powder agent:50g, wherein containing 5.5 parts of Bifidobacterium, 1.5 parts of Lactobacillus rhamnosus in per g probiotic powders, doing
2.6 parts of 2.3 parts of Lactobacillus paracasei, 2.6 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Integration of drinking and medicinal herbs food materials composition:100g, is formed wherein being extracted per g medicine baking soda agent by the raw material of following portions by weight:Mountain
25 parts of 20 parts of medicine, 25 parts of longan, 26 parts of agate card, 20 parts of jujube, 12 parts of matrimony vine and acerola concentrate powder;
Preparation method:Prepared according to the preparation method of embodiment 2, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 5
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 83g, soy oligosaccharides Icing Sugar 61g, FOS 56g;
Probiotics powder agent:20g, wherein containing 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva in per g probiotic powders
2.2 parts of 0.9 part of lactobacillus, 1.5 parts of lactobacillus acidophilus and Lactobacillus plantarum;
Integration of drinking and medicinal herbs food materials composition:50g, is formed wherein being extracted per g medicine baking soda agent by the raw material of following portions by weight:Chinese yam
18 parts of 15 parts, 22 parts of longan, 22 parts of agate card, 13 parts of jujube, 10 parts of matrimony vine and acerola concentrate powder;
Resistant dextrin 300g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 6
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 43g, mannan-oligosaccharides 40g, FOS 23g, galactooligosaccharide 22g and
Trehalose 19g;
Probiotics powder agent:10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, mouse in per g probiotic powders
2.4 parts of lactobacillus of Lee's sugar, 1.3 parts of Lactobacillus helveticus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and secondary cheese breast
0.9 part of bacillus;
Integration of drinking and medicinal herbs food materials composition:4.55g, is formed wherein being extracted per g medicine baking soda agent by the raw material of following portions by weight:Mountain
12 parts of 7 parts of medicine, 12 parts of longan, 10 parts of agate card, 10 parts of jujube, 5 parts of matrimony vine and acerola concentrate powder;
Resistant dextrin 70.5g
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 7
The pulvis fermentation composition 32g that embodiment 1 is made;Saccharomycete 1g;Acetobacter 1g;Lactic acid bacteria 1g;Coating solution by
Eudragit L 100-5520g, Hydroxypropyl Methylcellulose Phathalate 5g and the appropriate purifying of castor oil 2.5g additions
Water is prepared;
Preparation method:
(1) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The fermentation composition of embodiment 8
The prescription of the fermentation composition of the present embodiment is:
The pulvis fermentation composition 23.1g that embodiment 3 is made;Saccharomycete 3g;Acetobacter 3g;Lactic acid bacteria 5g;Coating solution
It is appropriate pure by Eudragit L 100-5510g, Hydroxypropyl Methylcellulose Phathalate 2g and castor oil 0.2g addition
Change water to prepare;
Preparation method:
(2) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The plant enzyme of embodiment 9
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 2 months, obtains plant enzyme stoste;
Wherein inoculum concentration is 12%;Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid
In bacillus;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g, and clean section is used.
Organoleptic parameters:The plant enzyme uniform color, free from admixture, in bronzing, there is strong fruity and fermentation fragrance, mouth
Sense is mellow, and comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 10
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 2;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:Add plant of fermentation composition of the weight for 60g step a) preparations into step b)
In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip
140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.
Organoleptic parameters:The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant
Gas, mellow in taste, comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 11
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 4;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 0.5kg, the plant enzyme stoste for adding 150ml are fermented in closed environment, and fermentation temperature is 28
~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is not
Become, continue the 3-4d that ferments, filtering produces plant enzyme.
Wherein, plant material selects passion fruit 120g, citrus 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery
40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.
Organoleptic parameters:The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 12
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 5;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:The fermentation composition for the step a) preparations that weight is 30~60g is added into step b)
Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 13
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, addition 5~
6L pure water, is well mixed, while add in fermentation composition to the ferment bucket that weight is prepared for 20~50g step a),
Capping, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
C) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 4~6d of fermentation, filtering is produced
Plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 14
The plant enzyme of the present embodiment is prepared from by following methods:
I the fermentation composition of pellet) is prepared according to embodiment 7
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
Observation to the pellet fermentation composition fermentation process of embodiment 7:Pellet fermentation composition during the fermentation, ball
The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten
Solution.
The plant enzyme of comparative examples 1
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
B) mixing and initial fermentation:It is inoculated with plant extraction liquid of the compound probiotic into step b), mixing is placed in closed
Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
C) zymotic fluid carries out after-ripening 7d in closed environment, takes Partial fermentation liquid to filter, does organoleptic parameters;Continue after-ripening
14d, takes Partial fermentation liquid to filter, does organoleptic parameters.
Wherein, compound probiotic is Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus and plant
Lactobacillus;Inoculum concentration is 8% (mass percent);
Plant material selects apple 500g, soybean 200g and tomato 300g.
The organoleptic parameters of zymotic fluid after 7 days:The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has
Stimulation, sweet taste are heavier;The organoleptic parameters of zymotic fluid after 14 days:The plant enzyme uniform color, in bronzing, there is strong
Fruity is not stimulated with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method
The fermentation time that ferment needs is longer than the fermentation time of plant enzyme of the present invention.
Comparative examples 2
The prescription of the fermentation composition of the present embodiment is same as Example 1
Fermentation composition preparation method:
1) preparation of integration of drinking and medicinal herbs food materials pulvis:
Chinese yam is cleaned, peeling simultaneously segment;Longan removes shell stoning, dries;Dried after Removing Kernel for Date;Weight is weighed after processing
Number Chinese yam, longan and jujube, it is stand-by;
By water, (stand-by Chinese yam, longan and jujube is 1 with water ratio:1.5) stand-by Chinese yam, osmanthus are placed into after boiling
Beater is placed in after circle and jujube, 8~12min to be beaten;Feed liquid after mashing, is preheated to 70~80 DEG C, under 25MPa
It is concentrated in vacuo after matter, homogeneous, is spray-dried, produces fine powder 1;
By the water of 8 times of weight of matrimony vine and Ma Ka of predetermined weight part, 4h is kept at 80-90 DEG C of temperature, is then cooled
To 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering, obtain the first filtrate;The water of 3 times of weight of filter residue is added,
2.5h is kept at 80-90 DEG C of temperature, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, freezes and do
Dry, low-temperature grinding continues to be ground into 0.05-0.2mm fine powder 2 into coarse powder;
Fine powder 1, fine powder 2 and acerola concentrate powder are well mixed, integration of drinking and medicinal herbs food materials pulvis is produced;2) by predetermined weight part
Several functional oligose, probiotics powder agent, integration of drinking and medicinal herbs food materials pulvis and resistant dextrins are uniformly mixed, and mixed powder are added dry
Method comminutor is granulated, and the granule of fermentation composition, pack, every bag of 30g is made.
Plant enzyme preparation method:Method according to embodiment 9 is prepared into plant enzyme.
Organoleptic parameters:The plant enzyme color and luster is uneven, muddy, has floating object, in bronzing, fruit flavor is thin, and entrance is not
Stimulate
The viable bacteria survival detection of the fermentation composition of test example 1
Fermentation composition prepared by the embodiment of the present invention 1, embodiment 7 and comparative examples 2, is prepared with sterilized water and survived
Bacterium number is 2 × 108CFU/mL solution to be measured, is saved backup in 4 DEG C;
The 10mL kept the solution to be measured of going bail for is injected into test tube 1, and 10 are diluted to step by step using ten times-8, take 1mL to dilute
The MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured on flat board (sterilizing), shaken up rapidly on flat board by liquid.Again will
The test tube 2 of solution to be measured equipped with 10mL, which is placed in 80-90 DEG C of water-bath, heats 15-25min, takes the solution to be measured after heating to enter
Row ten is diluted to 10 step by step again-8, 1mL dilutions are taken on flat board, and the MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured into
Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates
Number of viable before and after heating.
As a result show, Viable detection is respectively 91.5%, 92.3% and 58.2%.
The viable bacteria survival detection of the plant enzyme of test example 2
1st, simulate the gastric juice and the resistance test of intestinal juice
100g/L hydrochloric acid 16.4mL plus distilled water diluting is taken, it is respectively 1.5,2.5 and 3.5 to make pH value, take the dilute salt of 100mL
Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains simulate the gastric juice, and miillpore filter degerming (0.22 μm) is standby.Take
Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value is adjusted to 6.8 with 0.1moL/L sodium hydroxide solutions;Separately take tryptose
Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, miillpore filter is degerming
(0.22 μm) is standby.
The embodiment 9 kept that 1mL is kept and the plant enzyme solution of embodiment 14 is taken to be added to 9mL simulate the gastric juice
In (i.e. ten times step by step dilute), and it is rapid fully mix on the oscillator, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively
Nutrient solution is taken out when 1h, 2h, 3h, 4h and remaining viable count is counted immediately, is compared with former viable count, as a result table
Bright, Viable detection is respectively 97.9% and 98.4%.Then take the nutrient solution for digesting different time in simulated gastric fluid each
1mL, is inoculated in the simulated intestinal fluid that 9mL pH value is 6.8, is placed in 30-45 DEG C of quiescent culture 2-4h respectively, and respectively 0,3,
6th, 24h is sampled, and is determined its viable count, is compared with former viable count, it is 99% as a result to show Viable detection.
The strong kidney effect assessment of kidney tonifying of the plant enzyme of test example 3
We observe influence of the plant enzyme of the present invention to hypoimmunity mice immunologic function, to prove the present invention to immune
Power is improved has certain effect really, now that result of study is as follows:
1. experiment material plant enzyme of the present invention (self-control is prepared by the method for specific embodiment 9 and embodiment 14);Kidney tonifying
Brain-nourishing capsule (BSC, 0.27g/, pharmaceutical factory of northern Guangxi, lot number 040815) is diluted with distilled water into 0.2g/ml;Male and female elder brother
Bright kind of mouse (22~26g).
2. the influence pair rat blood serum total protein
The sick type Nephritis Model of rat blood serum is made first.Give rat tail vein injection calf serum (albumin amount 35mg/
Kg), conventinal breeding 7 days, collect urine on 8th.Urine proteins are detected with acetic acid heating, positive selects 60 rats, by sex
Ratio is divided into 6 groups.Nephritis Model rat is taken with ether light anaesthesia arteria carotis the light splitting light of serum 721 is taken after blood 3000rpm, centrifugation
Its optical density is determined under degree 46nm wavelength, the content for calculating total serum protein carries out the statistical procedures evaluation total egg of drugs on serum
Bai Hanliang influence.The seralbumin amount of the high low dose group of acetonideexample 9 and the high low dose group of embodiment 14 is significantly higher than
Control group.It the results are shown in Table 1.
Influence of the plant enzyme of the present invention of table 1 to Glomerulonephritis Rats total serum protein
Group | Dosage | Size of animal (only) | Total serum protein |
Control group | -- | 10 | 5.01±0.74 |
Prednisone | 0.2mg/kg | 10 | 7.43±3.4* |
The high dose group of embodiment 9 | 20ml/kg | 10 | 7.30±2.2* |
The low dose group of embodiment 9 | 5ml/kg | 10 | 7.16±2.6* |
The high dose group of embodiment 14 | 20ml/kg | 10 | 7.41±3.1* |
The low dose group of embodiment 14 | 5ml/kg | 10 | 7.22±0.7* |
Compared with control group:* P < 0.05
3. the influence pair sexual disorder mouse sexuality power:
22~26g of body weight male mices 60,6 groups, every group 10 are divided into by body weight;Female mice 60 is only also divided into 6
Group.The control group of male mice, daily gavage distilled water 20ml/kg, the same gavage distilled water of model control group is every from the 4th day
The double hind legs of mouse are suspended to above tank by day with cotton rope, and a certain amount of plain boiled water is contained in groove, are made the mouse nose contact water surface, are forced
Mouse, which comes back, to struggle, and one time a day, 30 minutes every time.The daily gavage capsule for tonifying kidneys and nourishing brain 10ml of positive controls is (dilute with distilled water
It is interpreted into 0.2g/ml concentration).The plant enzyme component of embodiment 9 is high (20ml/kg), low (5ml/kg) two dosage groups;Embodiment
14 plant enzyme components are high (20ml/kg), low (5ml/kg) two dosage groups;Administration group also started as previously described on 4th
Suspension stress, one time a day, continuous 5 days.Male mouse and female mice are mated 20 minutes by 1: 1 after daily suspention, observation hero mouse ride or
The incubation period for the female mice that mates and number of times.Experiment is mated to carry out 6 times altogether.The high low dose group of the plant enzyme of acetonideexample 9 and implementation
The incubation period of high four groups of low dose group of the plant enzyme of example 14 shortens, and number of times of the sexual behaviour in 20 minutes significantly increases,
And have certain dose-effect relationship.It the results are shown in Table 2 and table 3.
The plant enzyme of the present invention of table 2 influence (S, x ± s) preclinical on mouse sexual behaviour
Group | Dosage | Animal/only | 1 day | 2 days | 3 days |
Control group | -- | 10 | 501±174 | 485±194 | 490±200 |
Model group | -- | 10 | 645±189 | 689±187 | 664±231 |
Capsule for tonifying kidneys and nourishing brain | 10mg/kg | 10 | 466±145 | 337±214* | 354±174* |
The high dose group of embodiment 9 | 20ml/kg | 10 | 319±204** | 297±156** | 196±180** |
The low dose group of embodiment 9 | 5ml/kg | 10 | 468±233 | 335±115* | 306±215** |
The high dose group of embodiment 14 | 20ml/kg | 10 | 302±123** | 287±141** | 194±240** |
The low dose group of embodiment 14 | 5ml/kg | 10 | 362±200* | 314±102* | 286±134** |
Compared with model control group:* P < 0.05, * * P < 0.01
Influence (secondary/20min, x ± s) of the plant enzyme of the present invention of table 3 to mouse sexual behaviour number of times
Compared with model control group:* P < 0.05, * * P < 0.01
4. result
Result of study shows that plant enzyme high and low dose group of the present invention has suppression to the albuminuria of the sick type ephritis of rat blood serum
Make and use.Illustrate there is improvement result to injury of kidney.Plant enzyme high and low dose of the present invention weakens to suspension stress male mice
Sexual function have humidification.Above description of test tonifies the kidney to relieve mental strain oral liquid with improvement renal function and improvement animal sexual function
Effect.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that:Not
In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The scope of invention is limited by claim and its equivalent.
Claims (10)
1. a kind of fermentation composition for preparing the plant enzyme with the strong renal function of kidney tonifying, it is characterised in that the fementative composition
Thing is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight:Functional oligose 3~
0.01~10 part of 22 parts, 0.1~5 part of probiotics and integration of drinking and medicinal herbs food materials pulvis;
The integration of drinking and medicinal herbs food materials pulvis is prepared from by parts by weight for the raw material of following weight parts:1~20 part of Chinese yam, longan
2~25 parts of 2~25 parts, 1~16 part of agate card, 1~20 part of jujube, 0.5~12 part of matrimony vine and acerola concentrate powder;
The preparation method of the fermentation composition at least comprises the steps:
The functional oligose of predetermined weight number and integration of drinking and medicinal herbs food materials pulvis are uniformly mixed into sterilization, with probiotics after sterilization
Powder is well mixed, granulation, and the fermentation composition of powdery is made.
2. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition by following parts by weight component
Composition:0.1~5 part of 5~10 parts of functional oligose, 1~3 part of probiotics and integration of drinking and medicinal herbs food materials pulvis.
3. fermentation composition as claimed in claim 1, it is characterised in that the integration of drinking and medicinal herbs food materials pulvis is by parts by weight
The raw material of following weight parts is prepared from:6~15 parts of Chinese yam, 8~18 parts of longan, 5~12 parts of agate card, 6~15 parts of jujube, matrimony vine
3~9 parts and 8~18 parts of acerola concentrate powder.
4. fermentation composition as claimed in claim 1, it is characterised in that it is 10 that the fermentation composition, which also includes parts by weight,
~30 parts of resistant dextrin.
5. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is stachyose;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:4.5~6 parts of Bifidobacterium,
1~2.6 part of Lactobacillus rhamnosus, 2~2.8 parts of Lactobacillus casei, 2.3~3 parts of lactobacillus acidophilus and Lactobacillus plantarum 2.3~
3 parts, the viable bacteria content of the probiotics powder agent is 2 × 106~2 × 1010cfu/g。
6. such as fermentation composition according to any one of claims 1 to 5, it is 1 that the fermentation composition, which also includes parts by weight,
~3 parts of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight laggard
Row extrusion makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight, made
It is standby into softwood thing;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce pellet
Fermentation composition;
Wherein, the coating solution is by the adjacent benzene for 100-55,0.1-2 parts of the Eudragit L that parts by weight are 0.4-10 parts
Dioctyl phthalate hydroxypropyl methyl cellulose and 0.05-0.2 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step
Suddenly:
1) preparation of integration of drinking and medicinal herbs food materials pulvis:
Chinese yam is cleaned, peeling simultaneously segment;Longan removes shell stoning, dries;Dried after Removing Kernel for Date;Parts by weight are weighed after processing
Chinese yam, longan and jujube, it is stand-by;
By water, (stand-by Chinese yam, longan and jujube is 1 with water ratio:1.5) placed into after boiling stand-by Chinese yam, longan and
Beater is placed in after jujube, 8~12min to be beaten;Feed liquid after mashing, is preheated to 70~80 DEG C, the homogeneous under 25MPa,
It is concentrated in vacuo after homogeneous, is spray-dried, produces fine powder 1;
By the water of 8 times of weight of matrimony vine and Ma Ka of predetermined weight part, 4h is kept at 80-90 DEG C of temperature, 40 are then cooled to
DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering, obtain the first filtrate;The water of 3 times of weight of filter residue is added, in temperature
2.5h is kept at 80-90 DEG C, 30 DEG C are then cooled to, filtering, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried,
Low-temperature grinding continues to be ground into 0.05-0.2mm fine powder 2 into coarse powder;
Fine powder 1, fine powder 2 and acerola concentrate powder are well mixed, integration of drinking and medicinal herbs food materials pulvis is produced;
2) by step 1) in integration of drinking and medicinal herbs food materials pulvis mixed with emulsifying agent, water after carried out with cutter 3000-5000 turn
High speed shear, is added dropwise wall material monomer and carries out wall building, form nano level particulate while stirring;
3) by the functional oligose, resistant dextrin and step 2 of predetermined weight number) in particulate uniformly mix, pass through heat exchange
Device is sterilized, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, and adding dry method after being well mixed makes
Grain machine granulation, is made the fermentation composition of powdery, sterile packing is produced.
8. a kind of preparation method of the plant enzyme with the strong renal function of kidney tonifying as any one of claim 1-5, it is special
Levy and be, the described method comprises the following steps:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5~6L
Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope
Lid, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering produces plant
Thing ferment.
9. a kind of preparation method of the plant enzyme with the strong renal function of kidney tonifying as any one of claim 1-5, it is special
Levy and be, the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carries out lucifuge
Fermentation 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, gram
It is several in lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with
And plant material 0.5kg, add 150ml plant enzyme stoste fermented in closed environment, fermentation temperature be 28~30
DEG C, deflate 1~2 time daily, after 1~2d of fermentation;Fermentation composition prepared by 30g step a) is added, fermentation temperature is constant, after
Supervention ferment 3-4d, filtering, produces plant enzyme.
10. a kind of preparation method of the plant enzyme with the strong renal function of kidney tonifying as claimed in claim 6, it is characterised in that institute
The method of stating comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water
And plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, sent out in closed environment
Ferment;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, and ventilate 2-4 daily
It is secondary, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
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