CN106858607A

CN106858607A - Prepare the fermentation composition and preparation method with the plant enzyme nursed one's health and strengthen immune function of human body

Info

Publication number: CN106858607A
Application number: CN201710233639.8A
Authority: CN
Inventors: 姜红成
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-04-11
Filing date: 2017-04-11
Publication date: 2017-06-20

Abstract

The present invention provides a kind of with the fermentation composition for nursing one's health and strengthening immune function of human body, is made up of yeast beta glucan, functional oligose, probiotics and integration of drinking and medicinal herbs food materials；Integration of drinking and medicinal herbs food materials is mainly prepared from by the raw material of following weight portion：10~45 parts of acerola concentrate powder and 3~26 parts of caterpillar fungus cephalosporin powder.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", suitably the industrialized production of all kinds of plant enzymes and family making.

Description

Prepare the fermentation composition with the plant enzyme nursed one's health and strengthen immune function of human body And preparation method

Technical field

The invention belongs to prevent conditioning maintenance product field, more particularly to one kind is prepared with conditioning and enhancing human immunity work( The fermentation composition and preparation method of energy.

Background technology

Plant enzyme is always the best seller of ferment class product.Plant enzyme is by tens of kinds of different vegetables and fruits, cereal, seas The plant such as algae and mushroom class, develops through spontaneous fermentation, remains the nutrition elite in plant, contains the various of human body needs Ferment, oligosaccharides and various vitamins and mineral matter, can produce abundant SOD antioxidase compositions again, improve anti-oxidant energy in vivo Power, and then strengthen immunity.

Existing traditional handicraft and modern crafts to ferment has all done further improvement, while also occurring in that some addition benefits Health food prepared by the ferment and ferment of raw bacterium.Probiotics fermention fruits and vegetables, can activate the effective efficiency composition in fruits and vegetables, molten Go out active effect material of more elite.But, traditional ferment technique cannot be used flexibly on a large scale, or without effect fermentation Composition can flexibly be used in the industrial manufacturing processes of different ferment；Promoting metabolism in vivo, adjusting to improve ferment The effect of the aspects such as reason functions of intestines and stomach, and adaptation is used on a large scale, and applicant further changes to the fermentate for preparing ferment It is good, and develop the formula of the function affect for improving ferment.

Additionally, functional oligose is a class have physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degrade, not small intestine inhale Receive, the oligosaccharide of big intestines can be reached；Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, and with promoting, human body is prebiotic The physiological functions such as the propagation of bacterium.It is used in conjunction with by by probiotics and functional oligose, between the two with synergy, energy Promote the regulatory function to gut flora.Co between probiotics and oligosaccharide, be probiotic in symphysis unit and Co between prebiotics, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria Growth；Research shows that both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no This effect.Such as the enzyme composition that patent CN106174179A is related to, probiotics and functional oligose are used in combination, and Addition traditional Chinese medicine ingredients, reach its given efficacy.But the preparation of its formula is applied to individual and directly takes, and fixed plant enzyme Stoste cannot meet different taste Man's Demands when reusing, and its preparation method is coarse, in the ferment effervescent tablet being prepared into The sense of the medicine dregs of a decoction is strong, and mouthfeel is not good.

On the other hand, existing ferment bucket, prepares plant enzyme so that plant enzyme by raw material of fruit, sugar and water Can be prepared in family, it is easy to operate.But also there are many weak points, the time that 1. prepared by ferment is still more long, Er Qierong Easily prepare failure；2. plant enzyme effect is indefinite obtained in, user cannot oneself control prepare effect of plant enzyme, it is existing Some plant enzymes are not good to the improvement of immune function of human body；3. low molecular sugar and polysaccharide in the plant such as fruit is most Cannot utilize, it is impossible to be converted into the oligosaccharide of given efficacy.For the deficiency, applicant puts forth effort research has shortening ferment system Standby time, fermentation composition easy to use and with conditioning and enhancing immune function of human body, i.e. ferment bag.It is simultaneously raising ferment The feature of element, adds effective integration of drinking and medicinal herbs food materials composition in the ferment fermentate of prior art, the ferment for preparing it Being capable of effective regulating intestinal canal flora, strengthen immunity, and with function.

The content of the invention

It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention It is to propose a kind of fermentation composition prepared with conditioning and enhancing immune function of human body, is directly putting type fermented microbial inoculum, the hair Be used in combination for probiotics and functional oligose by ferment composition, while adding integration of drinking and medicinal herbs food materials composition, said composition is existed While with good gut flora regulatory function, the conversion of small molecular sugar and polysaccharide to oligosaccharide can be promoted.With this Plant enzyme prepared by fermentation composition plays a part of to nurse one's health and strengthen immune function of human body.

Technical scheme is as follows：

It is a kind of to prepare the fermentation composition with the plant enzyme nursed one's health and strengthen immune function of human body, it is direct putting type plant Ferment fermenting agent, the fermentation composition includes the component of following parts by weight：0.5~15 part of polysaccharide, functional oligose 3 0.01~10 part of~22 parts, 0.1~5 part of probiotics and integration of drinking and medicinal herbs food materials,

Wherein, the polysaccharide is yeast beta-dextran；

The integration of drinking and medicinal herbs food materials is mainly prepared from by the raw material of following weight portion：10~45 parts of acerola concentrate powder with And 3~26 parts of caterpillar fungus cephalosporin powder；

The preparation method of the fermentation composition at least comprises the steps：

The particulate fine powder that the polysaccharide of predetermined weight number, functional oligose and integration of drinking and medicinal herbs food materials are made uniformly is mixed Close sterilized, be well mixed with probiotic powder after sterilization, granulate, the fermentation composition of powdery is obtained.

In the program, the functional oligose is low selected from shitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew Glycan, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, sea One or more in algae sugar, palatinose and inulin；

Probiotics is selected from Bifidobacterium (Bifidobacterium), Lactobacillus rhamnosus (L.rhamnosus), cheese breast Bacillus (Lactobacillus casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus Plantarum), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus Helveticus), Bu Shi lactobacillus (Lactobacillus buchneri), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), lactobacillus paracasei (Lactobacillus paracasei) and Luo Yishi breast bars One or more in bacterium (Lactobacillus reuteri)；

Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity；And combining yeast beta glucan Use can effective adjusting body digested road Tiny ecosystem, promote the excretion of nuisance in the propagation and enteron aisle of internal beneficial bacterium； Internal cholesterol level can be reduced, internal low-density lipoprotein content is reduced, and increase the content of HDL；Can improve The ability of the infection such as body resistance virus, bacterium.Coordinate the use of acerola concentrate powder and caterpillar fungus cephalosporin powder, be capable of achieving conditioning With enhancing immune function of human body；Acerola concentrate not only Vc rich content, while vitamin A, E, P, vitamin B complex, egg can be provided White matter and the necessary multi mineral trace element of various human bodies；And the non-specific immune function tool of caterpillar fungus cephalosporin powder antagonist Have certain effect, and the specific immunity ability of collective can also be significantly improved, be a kind of good enhancing immune drug.Separately On the one hand, fermentation composition is made powdery, implementation phase formula is produced, being easy to the direct plunge into use or family oriented in later stage makes With.

Further, the integration of drinking and medicinal herbs food materials also raw material including following weight parts is prepared from：3~20 parts of Fu Siberian cocklebur, 2~18 parts of matrimony vine, 3~20 parts of mulberries, 1~10 part of white fungus, 3~20 parts of date and 2~20 parts of Pu are public English.

Further, the fermentation composition is by yeast beta-dextran, 5~10 parts of function that parts by weight are 2~6 parts Property oligosaccharide, the integration of drinking and medicinal herbs food materials composition of 1~3 part of probiotics and 0.5~1 part；

Wherein, the integration of drinking and medicinal herbs food materials is prepared from by the raw material of following weight portion：20~35 parts of acerola concentrate powder, 8~14 parts of caterpillar fungus cephalosporin powder, 9~15 parts of Poria cocos, 6~12 parts of matrimony vine, 9~15 parts of mulberries, 4~7 parts of white fungus, 6~12 parts of date and 9~15 parts of dandelion.

The preferred scheme of fermentation composition of the invention, also including the resistant dextrin that parts by weight are 10~30 parts, resistance Dextrin is used in conjunction with other components, further promotes intestines peristalsis, keeps intestinal health, and control the absorption of grease and sugar.

Further, the functional oligose is galactooligosaccharide；

The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：Bifidobacterium 45~ 60 parts, 50~60 parts of Lactobacillus rhamnosus, 22~30 parts of Lactobacillus casei, 18~26 parts of lactobacillus acidophilus and Lactobacillus plantarum 23~30 parts, the viable bacteria content of the probiotics powder agent is 2 × 10⁶~2 × 10¹⁰cfu/g；By all kinds of strains of reasonable disposition and Consumption, optimizes the strain of this patent products'texture, can effectively shorten fermentation composition in the time for preparing plant enzyme, and can make Its plant enzyme for preparing texture stabilization.

The source of above-mentioned all kinds of probiotics powder agents：It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of Strain is collected by centrifugation with after washing thalline through Liquid Culture, and drying is prepared into active powder.

In the one of preferred scheme of fermentation composition of the invention, the fermentation composition also includes that parts by weight are 1 ~3 parts of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria；

The preparation method of the fermentation composition also includes：

(1) fermentation composition of the powdery of 1/2 amount is made softwood, the lactic acid bacteria with the weight portion is well mixed After carry out extrusion and make ball, be made capsule core；

(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the weight portion It is even, softwood thing is made, it is standby；

(3) obtained capsule core is placed in the coating solution of advance preparation, is coated；

(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product Pellet fermentation composition；

Wherein, it by parts by weight is Eudragit L 100-55,0.1-2 part of 0.4-10 parts that the coating solution is The aqueous dispersion that Hydroxypropyl Methylcellulose Phathalate and 0.05-0.2 parts of castor oil add appropriate purified water to be configured to.

The preparation method of fermentation composition of the present invention, comprises the following steps：

1) preparation of integration of drinking and medicinal herbs food materials：

The Poria cocos of the weight portion is crushed, 60 mesh sieves are crossed, Indian Bread is obtained；

By matrimony vine, mulberries, white fungus, date, the dandelion of Indian Bread and predetermined weight part with 8 times of water of weight, in temperature 4h is kept at 80-90 DEG C of degree, 40 DEG C are then cooled to, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering obtains first Filtrate；Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, filtering, the second filter Liquid；Merging filtrate, is concentrated under reduced pressure, freeze-drying, and low-temperature grinding is into meal；

Meal is well mixed with acerola concentrate powder, caterpillar fungus cephalosporin powder, continues to be ground into the fine powder of 0.05-0.2mm；

2) by step 1) in fine powder mix with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear, Wall material monomer is added dropwise while stirring carries out wall building, forms nano level particulate；

3) by the functional oligose of predetermined weight number, yeast beta-dextran, resistant dextrin and step 2) in particulate Uniform mixing, sterilization is carried out by heat exchanger, and condition is 99 DEG C, 300s；

4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, is added after being well mixed dry Method granulator granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.

Fermentation composition viable bacteria rate prepared by the above method is high, the traditional Chinese medicine fine powder delicate mouthfeel of particulate treatment, Chinese medicine particulate The capsule heart is slowly discharged, with good slow release effect by processes such as dissolving, infiltration, diffusions through membranous wall.

A further object of the present invention is to provide a kind of plant enzyme with conditioning and enhancing immune function of human body, is pin Personal or Homemade plant enzyme is formulated, the preparation method of the plant enzyme is comprised the following steps：

A () prepares powdery fermentation composition

(b) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, add 5 The pure water of~6L, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket Interior, capping carries out lucifuge fermentation in the environment that fermentation temperature is 28~30 DEG C；

C () is fermented：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 5~8d of fermentation, filtering, i.e., Obtain plant enzyme.

The method simple and fast, realizes the self-control of various functions plant enzyme, and the method preferably uses ferment bucket and made Standby, ferment bucket is from market sale；Also the vessel of closed environment are can be used to be prepared.

Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps：

A) fermentation composition of powdery is prepared

B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added Enter the pure water of 10L, be well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filter, obtain plant extraction liquid；

C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 1~2 month, obtains plant enzyme stoste；

Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, leather It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter；

D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is not Become, continue the 3-4d that ferments, filtering obtains final product plant enzyme.

Which is applied to industrial production, secondary with fermentation composition of the present invention with reference to plant enzyme stoste first fermentation Multistage deep layer liquid state fermentation is realized in fermentation；Greatly improve fermentation efficiency.Be can release in fermentation process and be enclosed in Chinese medicine or plant Structure and intracellular nutrients element, retain the original active material of plant, and insoluble fibre is changed into solvable in plant Property dietary fiber improve effect of original plant.

The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition for also being constructed for double-deck pellet Method, the preparation method is comprised the following steps：

I the fermentation composition of pellet) is prepared

II) the preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and The pure water of 10L is added, is well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filtered, obtain plant extraction liquid；

III) the preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water and plant extraction liquid 1kg, add 30g the step of II) fermentation composition of pellet for preparing, carried out in closed environment Fermentation；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；After 1~2d of fermentation, insertion conduit is passed through air, daily ventilation 2-4 times, each 10-30min, and it is 35~37 DEG C to control fermentation temperature；Filtered after 5~7d of fermentation, obtain final product plant enzyme.

The method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation Realize second order fermentation, or even multistage fermentation；The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries Its effect high.

The present invention has the following advantages that compared with prior art：

The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly be existed with the bacterium bag of granule Sell on the market, can also put into the industrialized production of all kinds of plant enzymes, realize that matching somebody with somebody for plant enzyme use and diversification Functional configuration, is greatly enhanced the preparation efficiency of plant enzyme.

The integration of drinking and medicinal herbs food materials component added in fermentation composition of the invention has its physiologically active and health-care effect, together When supplemented the nutrients in fermentation for probiotics, with practical value higher, and integration of drinking and medicinal herbs food materials composition of the invention has The effect of enhancing immunity of organisms；Compatibility rationally, is treated between probiotics, functional oligose and integration of drinking and medicinal herbs food materials each component Effect is clear and definite, the effect with Synergistic.

Plant enzyme of the invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body The comprehensive plant enzyme of benefit, these enzymes play an important role to the metabolism of human body and function adjustment, while also containing vitamin, mineral The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group Integration of drinking and medicinal herbs food materials composition in compound is changed through probiotics, and small molecule is become by macromolecular, is easily absorbed by the body；Probiotics Addition can improve integration of drinking and medicinal herbs food materials taste, toxicity can be decomposed in fermentation process, realize it is real have no toxic side effect, while should Plant enzyme prepared by fermentation composition improves drug effect compared to traditional Chinese medicine preparation；Additionally, it takes as daily drinks With, improve lipid-metabolism from the angle effected a permanent cure, the specific immunity ability of human body is significantly improved, it is that a kind of good enhancing is immunized Health care preparation.

Additionally, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.

The source of integration of drinking and medicinal herbs food materials material composition of the invention：

Acerola concentrate：It is Malpighiaceae (Malpighiaceae) Malpighia (Malpighia) concave edge Malpighia coccigera (M.emarginata) fruit.The present invention uses commercially available acerola concentrate powder.

Caterpillar fungus cephalosporin powder：Isolated Chinese caterpillar fungus cephalo new strains from natural cordyceps fresh specimens, using life Thing engineering technology is refined to be formed.The present invention uses commercially available caterpillar fungus cephalosporin powder.

Poria cocos：This product is the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf.

Matrimony vine：This product is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..

Mulberries：This product dries fruit ear for moraceae plants mulberry Morus alba L.'s.

White fungus：Mycophyta Tremellaceae white fungus platymiscium white fungus Tremellafuciformis Berk. are used as medicine with fructification.

Date：This product is Rhamnaceae jujube jujube Ziziphus jujuba Mill.var.inermis (Bunge) Rehd. dry mature fruit.

Dandelion：This product is feverfew dandelion Taraxacum mongolicum Hand.-Mazz., alkali ground Pu public affairs English Taraxacum sinicum Kitag. or belong to several plants together dry herb.

Specific embodiment

The fermentation composition of embodiment 1

The prescription of the fermentation composition of the present embodiment is：

Polysaccharide：Yeast beta-dextran 48g

Functional oligose：Galactooligosaccharide 80g

Probiotics powder agent：24g, wherein containing 4.5 parts of Bifidobacterium, 5 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders 2.3 parts of 2.2 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Integration of drinking and medicinal herbs food materials composition：8g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle cherry 2 parts of peach powder, 0.8 part of caterpillar fungus cephalosporin powder, 0.9 part of Poria cocos, 0.6 part of matrimony vine, 0.9 part of mulberries, 0.4 part of white fungus, 0.6 part of date with And 0.9 part of dandelion；

Resistant dextrin 80g.

Preparation method：

1) preparation of integration of drinking and medicinal herbs food materials：

4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, is added after being well mixed dry Method granulator granulation, is obtained the fermentation composition of powdery, aseptic pack, every bag of 30g.

The fermentation composition of embodiment 2

Polysaccharide：Yeast beta-dextran 40g

Functional oligose：Galactooligosaccharide 100g

Probiotics powder agent：20g, wherein containing 6 parts of Bifidobacterium, 6 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders 3 parts of 3 parts of bacillus, 2.6 parts of lactobacillus acidophilus and Lactobacillus plantarum；Integration of drinking and medicinal herbs food materials composition：10g, wherein per g extracts by The raw material of following portions by weight is extracted and formed：3.5 parts of acerola concentrate powder, 1.4 parts of caterpillar fungus cephalosporin powder, 1.5 parts of Poria cocos, matrimony vine 1.5 parts of 1.2 parts, 1.5 parts of mulberries, 0.7 part of white fungus, 1.2 parts of date and dandelion.

Preparation method：

1) preparation of integration of drinking and medicinal herbs food materials：

3) by the functional oligose of predetermined weight number, yeast beta-dextran and step 2) in particulate uniformly mix, Sterilization is carried out by heat exchanger, condition is 99 DEG C, 300s；

The fermentation composition of embodiment 3

Polysaccharide：Yeast beta-dextran 10g

Functional oligose：Galactooligosaccharide 60g

Probiotics powder agent：2g, wherein containing 5.5 parts of Bifidobacterium, 5.6 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders 2.6 parts of 2.5 parts of lactobacillus, 2 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Integration of drinking and medicinal herbs food materials composition：0.2g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle 1 part of cherry powder, 0.3 part of caterpillar fungus cephalosporin powder, 0.3 part of Poria cocos, 0.2 part of matrimony vine, 0.3 part of mulberries, 0.1 part of white fungus, 0.3 part of date And 0.2 part of dandelion；

Resistant dextrin：200g.

Preparation method：Prepared by the preparation method according to embodiment 1, pulvis, every bag of 30g is obtained.

The fermentation composition of embodiment 4

Polysaccharide：Yeast beta-dextran 150g

Functional oligose：Shitosan 104g, mannan-oligosaccharides 116g；

Probiotics powder agent：50g, wherein in per g probiotic powders containing 5.5 parts of Bifidobacterium, 2.4 parts of lactobacillus bulgaricus, 2.4 parts of 1.7 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Integration of drinking and medicinal herbs food materials composition：100g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle 4.5 parts of cherry powder, 3 parts of caterpillar fungus cephalosporin powder, 2 parts of Poria cocos, 1.8 parts of matrimony vine, 2 parts of mulberries, 1 part of white fungus, 2 parts and Ma Pu of date 2 parts of public English.

Preparation method：Prepared by the preparation method according to embodiment 2, pulvis, every bag of 30g is obtained.

The fermentation composition of embodiment 5

Polysaccharide：Yeast beta-dextran 80g

Functional oligose：Xylo-oligosaccharide 83g, soy oligosaccharides Icing Sugar 61g, FOS 56g；

Probiotics powder agent：20g, wherein containing 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva in per g probiotic powders 2.2 parts of 0.9 part of lactobacillus, 1.5 parts of lactobacillus acidophilus and Lactobacillus plantarum；

Integration of drinking and medicinal herbs food materials composition：50g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle cherry 3.5 parts of peach powder, 1.6 parts of caterpillar fungus cephalosporin powder, 1.5 parts of Poria cocos, 1.2 parts of matrimony vine, 0.9 part of mulberries, 0.7 part of white fungus, 0.6 part of date And 0.8 part of dandelion；

Resistant dextrin：300g

The fermentation composition of embodiment 6

Polysaccharide：Yeast beta-dextran 72g

Functional oligose：Xylo-oligosaccharide 43g, mannan-oligosaccharides 40g, FOS 23g, galactooligosaccharide 22g and Trehalose 19g；

Probiotics powder agent：10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, mouse in per g probiotic powders 2.4 parts of lactobacillus of Lee's sugar, 1.3 parts of Lactobacillus helveticus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and secondary cheese breast 0.9 part of bacillus；

Integration of drinking and medicinal herbs food materials composition：4.55g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle 2.1 parts of cherry powder, 1.3 parts of caterpillar fungus cephalosporin powder, 0.8 part of Poria cocos, 1.2 parts of matrimony vine, 1 part of mulberries, 0.9 part of white fungus, 1.6 parts of date And 1.2 parts of dandelion；

Resistant dextrin 70.5g.

The fermentation composition of embodiment 7

Polysaccharide：Yeast beta-dextran 48g

Functional oligose：Galactooligosaccharide 80g

Integration of drinking and medicinal herbs food materials composition：8g, wherein extracted by the raw material of following portions by weight per g extracts forming：Needle cherry 2 parts of peach powder, 0.8 part of caterpillar fungus cephalosporin powder.

Preparation method：

1) bodkin leaf cherry powder, caterpillar fungus cephalosporin powder are well mixed, continue to be ground into the fine powder of 0.05-0.2mm；

The fermentation composition of embodiment 8

The pulvis fermentation composition 32g that embodiment 1 is made；Saccharomycete 1g；Acetobacter 1g；Lactic acid bacteria 1g；Coating solution by Eudragit L 100-5520g, Hydroxypropyl Methylcellulose Phathalate 5g and the appropriate purifying of castor oil 2.5g additions Water is prepared；

Preparation method：

(1) the pulvis fermentation composition of 1/2 amount is made softwood, after being well mixed with the lactic acid bacteria of the weight portion Carry out extrusion and make ball, be made capsule core；

(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the weight portion, Softwood thing is made, it is standby；

(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product Pellet fermentation composition, packaging.

Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core；It is preferred that pellet diameters 5mm, capsule core is a diameter of 2.8mm。

The fermentation composition of embodiment 9

The pulvis fermentation composition 23.1g that embodiment 3 is made；Saccharomycete 3g；Acetobacter 3g；Lactic acid bacteria 5g；Coating solution It is appropriate pure by Eudragit L 100-5510g, Hydroxypropyl Methylcellulose Phathalate 2g and castor oil 0.2g addition Change water to prepare；

Preparation method：

(2) the pulvis fermentation composition of 1/2 amount is made softwood, after being well mixed with the lactic acid bacteria of the weight portion Carry out extrusion and make ball, be made capsule core；

The plant enzyme of embodiment 10

The plant enzyme of the present embodiment is prepared from by following methods：

A) preparation of fermentation composition:It is prepared by the fermentation composition according to embodiment 1；

C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 2 months, obtains plant enzyme stoste；

Wherein inoculum concentration is 12%；Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid In bacillus；

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g, and clean section is used.

Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.

The plant enzyme of embodiment 11

A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 2；

B) preparation of plant extraction liquid：The plant material of 1kg is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and add 5~6L pure water, be well mixed, stand extraction 1~2d after, carry out high-temperature sterilization, filter, obtain plant extract Liquid；

C) mixing and initial fermentation：Plant in fermentation composition to step b) prepared by the step of addition weight is 60g a) In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, obtains final product plant enzyme.

Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip 140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.

Organoleptic parameters：The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant Gas, mellow in taste, comfortable acid, entrance does not stimulate, and pleasant impression is sweet.

The plant enzyme of embodiment 12

A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 3；

C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 1 month, obtains plant enzyme stoste；

Wherein, plant material selects passion fruit 120g, oranges and tangerines 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery 40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.

Organoleptic parameters：The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.

The plant enzyme of embodiment 13

A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 5；

C) mixing and initial fermentation：Fermentation composition prepared by the step of addition weight is 30~60g a) is in step b) Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

The plant enzyme of embodiment 14

A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 1；

B) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, addition 5~ The pure water of 6L, is well mixed, while add in fermentation composition to the ferment bucket that the step of weight is 20~50g prepared by a), Capping, lucifuge fermentation is carried out in the environment that fermentation temperature is 28~30 DEG C；

C) ferment：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 4~6d of fermentation, filtering is obtained final product Plant enzyme.

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

The plant enzyme of embodiment 15

I the fermentation composition of pellet) is prepared according to embodiment 8

Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.

Observation to the pellet fermentation composition fermentation process of embodiment 8：Pellet fermentation composition during the fermentation, ball The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten Solution.

The plant enzyme of comparative examples 1

A) preparation of plant extraction liquid：The plant material of 1kg is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and add 5~6L pure water, be well mixed, stand extraction 1~2d after, carry out high-temperature sterilization, filter, obtain plant extract Liquid；

B) mixing and initial fermentation：In plant extraction liquid in inoculation compound probiotic to step b), mixing is placed in closed Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；

C) zymotic fluid carries out after-ripening 7d in closed environment, takes the filtering of Partial fermentation liquid, does organoleptic parameters；Continue after-ripening 14d, takes the filtering of Partial fermentation liquid, does organoleptic parameters.

Wherein, compound probiotic is Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus and plant Lactobacillus；Inoculum concentration is 8% (mass percent)；

Plant material selects apple 500g, soybean 200g and tomato 300g.

The organoleptic parameters of the zymotic fluid after 7 days：The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has Stimulation, sweet taste are heavier；The organoleptic parameters of the zymotic fluid after 14 days：The plant enzyme uniform color, in bronzing, there is strong Fruity does not stimulate with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method The fermentation time that ferment needs is longer than the fermentation time of plant enzyme of the present invention.

Comparative examples 2

The prescription of the fermentation composition of the present embodiment is same as Example 1

Fermentation composition preparation method：

(1) according to the step 1 of embodiment 1) prepare integration of drinking and medicinal herbs food materials：

(2) by the integration of drinking and medicinal herbs food materials of predetermined weight number, functional oligose, yeast beta-dextran, probiotics powder agent, Resistant dextrin uniformly mixes, and mixed powder is added into dry granulating machine granulation, and the granule of fermentation composition, pack, every bag is obtained 30g。

Plant enzyme preparation method：Method according to embodiment 10 is prepared into plant enzyme.

(2) organoleptic parameters：The plant enzyme color and luster is uneven, muddy, there is floating object, and in bronzing, fruit flavor is thin, entrance Do not stimulate.

The viable bacteria survival detection of the fermentation composition of test example 1

Fermentation composition prepared by the embodiment of the present invention 1, embodiment 8 and comparative examples 2, is prepared with sterilized water and survived Bacterium number is 2 × 10⁸The solution to be measured of CFU/mL, saves backup in 4 DEG C；

The solution to be measured of the 10mL for keeping of going bail for is injected into test tube 1, using ten times of stepwise dilutions to 10^-8, take 1mL dilutions Liquid will be cooled to 45 DEG C of MRS agar mediums and pour on flat board (sterilizing) on flat board after sterilizing, shake up rapidly.Again will The test tube 2 of the solution to be measured equipped with 10mL is placed in 80-90 DEG C of water-bath and heats 15-25min, takes the solution to be measured after heating and enters Ten times of stepwise dilutions of row are to 10^-8, 1mL dilutions are taken on flat board, the MRS agar mediums that 45 DEG C will be cooled to after sterilizing are poured into Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates Number of viable before and after heating.

Result shows that Viable detection has reached and has been respectively 93.2%, 94.1% and 62%.

The viable bacteria survival detection of the plant enzyme of test example 2

1st, the resistance test of SGF and intestinal juice

The hydrochloric acid 16.4mL plus distilled water diluting of 100g/L are taken, pH value is respectively 1.5,2.5 and 3.5, take the dilute salt of 100mL Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains SGF, and miillpore filter degerming (0.22 μm) is standby.Take Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value to 6.8 is adjusted with 0.1moL/L sodium hydroxide solutions；Separately take tryptose Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, and miillpore filter is degerming (0.22 μm) is standby.

Take embodiment 10 that 1mL keeps and the plant enzyme solution of embodiment 15 is added to (i.e. ten in the SGF of 9mL Times stepwise dilution), and it is rapid fully mix on the oscillator, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively 1h, 2h, Nutrient solution is taken out when 3h, 4h and remaining viable count is counted immediately, is compared with former viable count, as a result shown, viable bacteria is deposited Motility rate is 98%.Then each 1mL of nutrient solution that different time is digested in simulated gastric fluid is taken, 9mL pH value is inoculated in respectively is In 6.8 simulated intestinal fluid, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h samplings, determine its viable count, with Former viable count is compared, and as a result shows that Viable detection is 99%.

The strengthen immunity Efficacy experiments of test example 3

1 material

1.1 given the test agent are plant enzyme obtained in embodiment 10

1.2 experimental animals and environment：SPF grades of KM female mice, body weight 18-22g；Experimental animal room temperature：22~25 DEG C, Relative humidity：55~70%.

2 methods and result

It is basic, normal, high that daily intaking amount 30ml/60kg bw (i.e. 0.5ml/1kg bw) according to people expand 5,10,30 times of settings Three dosage：2.5ml/kg bw、5ml/kg bw、15ml/kg bw；Negative control group is set simultaneously.Test group uses distilled water Given after deployed concentration, negative control group directly gives distilled water, gavage mouse 30 days.

The mouse spleen lymphocyte transformation experiment of 2.1ConA inductions

By prescribed dose to each group test mice continuous gavage 30d after, cervical dislocation put to death mouse, it is aseptic to take spleen, be made Single cell suspension (cell concentration is 1 × 107/mL).Mouse boosting cell suspension is separately added into 24 well culture plates, at each Reason uses two multiple holes, and per hole 1mL, a hole adds 50 μ LConA, the 50 μ L distilled waters that another hole adds then will as control Culture plate is placed in 5%CO₂, cultivated 48 hours in 37 DEG C of CO2gas incubator.Culture terminates preceding 4h, adds MTT (5mg/ ML) 50 μ L/ holes, continue to cultivate, and after culture terminates, 1mL DMSO are added per hole, and piping and druming is mixed, and is completely dissolved purple crystal, Then it is dispensed into 96 well culture plates, 3 multiple holes is dispensed per hole, OD values is determined at 570nm wavelength with ELIASA.With plus hole OD value subtracts and is not added with the OD value in hole and represents the multiplication capacity of lymphocyte.

The multiplication capacity of lymphocyte=ConA holes OD values-control wells OD values.

Influence (n=10) of the plant enzyme of table 1 to mouse lymphocyte conversion capability

From table 1, compare with control group, each dosage group OD values are above control group, and increase with dosage and increase, its Middle high, middle dose group OD values have significant difference (P ＜ 0.05 or P ＜ 0.01) compared with class value is compareed.This shows of the invention Plant enzyme has the effect of enhancing mouse lymphocyte conversion capability, i.e., with the effect of enhancing mouse cell immunologic function.

2.2 dinitrofluorobenzene (DNFB) induce delayed allergy (DTH) experiment

By prescribed dose to each group test mice continuous gavage 30d after, mouse web portion unhairing, with 1%DNFB acetone sesame oil The μ L of solution 50 smear sensitization, and 5d after sensitization, the μ L of mouse right ear uniform application 1%DNFB acetone sesame oil solution 10 are attacked, left Ear is smeared acetone sesame oil solution and is compared, and 24h puts to death mouse after attack, cuts left and right auricular concha, takes diameter 8mm's in same position Auricle is weighed, and the difference of left and right auricle weight is used as swelling.

Influence (n=10) of the plant enzyme of table 2 to mouse delayed allergy

As shown in Table 2, compared with control group, the low dosage of plant enzyme of the present invention influences unobvious to mice ear degree (P ＞ 0.05)；Middle dosage and high dose are obviously promoted rising effect (P ＜ 0.05=to mice ear degree.Plant enzyme pair The ear swelling degree of mouse delayed allergy has facilitation, i.e., with the effect of enhancing mouse cell immunologic function.

2.3 antibody-producting cell detections

By prescribed dose to each group test mice continuous gavage 30d after, per (v/v) hematocrit of mouse intraperitoneal injection 2% SRBC0.2mL sensitization.After immune 5d, mouse cervical dislocation is put to death.Spleen is taken out, single cell suspension is made, haemolysis is carried out empty Patch test.Hemolysis plaque number is counted, antibody-producting cell number is represented with plaque number/106 splenocyte.

Influence (n=10) of the plant enzyme of table 3 to mice spleen antibody-producting cell amount

As shown in Table 3, compare with control group, each dosage group plaque number is above control group, and increases with dosage and increase, Wherein high, middle dose group plaque number has significant difference (P ＜ 0.05) compared with class value is compareed.This shows that thing of the present invention has and increases The ability of strong mice spleen antibody-producting cell, i.e., with the effect of enhancing mouse humoral immune function.

The measure of 2.4 serum hemolysins

By prescribed dose to each group test mice continuous gavage 30d after, every chicken erythrocyte suspension of mouse peritoneal injection 5% 0.2mL is immunized, and after being immunized 7 days, angular vein clump takes blood, places 1 hour at room temperature, is divided solidification blood and tube wall with minute hand From with 2000r/min centrifugations 10min.100 times of serum normal saline dilution is taken, dilute serum 1mL is taken, with 5% chicken red blood cell Suspension 0.5mL, (GPS, is made into 10%) 0.5mL and mixes 10% complement with physiological saline, 37 DEG C of insulation 30min, 0 DEG C of end Only react.After centrifugation, supernatant spectrophotometer colorimetric at 540nm is taken, record OD value.

Influence (n=10) of the plant enzyme of table 4 to mice serum Hemolysin formation

From table 4, compared with control group, each dosage group of the invention is omited in the influence of mice serum Hemolysin formation Higher than control group, but there are no significant significant difference (P ＞ 0.05).Illustrate generation of the thing of the present invention to mice serum hemolysin Do not make significant difference.

2.5 mouse carbonic clearance tests

By prescribed dose to each group test mice continuous gavage 30d after, from the tail vein of mouse press 0.1mL/10g amount injection Concentration is 20% prepared Chinese ink, after interval 2min, 15min, takes the μ L of blood 20 from mouse angular vein clump respectively, is added to the 1% of 2mL Na₂CO₃In solution.Measured value OD values are distinguished at 600nm wavelength with photometer, with Na₂CO₃Solution makees blank.Then Cervical dislocation puts to death mouse, takes out liver and spleen, and the blood stains of organ surface are blotted with filter paper, weighs.OD values, the liver that will be measured Dirty and spleen weight substitutes into formula, calculates phagocytic index (ability of mouse carbonic clearance is represented with phagocytic index).

Influence (n=10) of the plant enzyme of table 5 to mouse macrophage carbonic clearance ability

From table 5, compare with control group, high, medium and low dosage group of the invention is to mouse macrophage carbonic clearance ability Phagocytic index is respectively provided with obvious rising effect (P ＜ 0.05).This explanation thing of the present invention has the phagocytosis for promoting mouse phagocyte Effect, can strengthen its non-specific immune function.

The phagocytosis chicken red blood cell experiment of 2.6 Turnover of Mouse Peritoneal Macrophages

By prescribed dose to each group test mice continuous gavage 30d after, to every mouse peritoneal injection chicken erythrocyte suspension, Activation mouse macrophage.After 4d, cervical dislocation puts to death mouse, and intraperitoneal injection adds the Hank's liquid 4mL/ of calf serum only, Abdominal cavity washing lotion is drawn to be mixed with 1% chicken red blood cell of equivalent.0.5mL mixed liquors are drawn, is added in the agar circle of slide, placement is incubated 37 DEG C are incubated 20 minutes in case.Incubation is washed out non-attached cell with physiological saline after terminating, and 1 minute is fixed in methanol solution, Giemsa liquid is dyeed 15 minutes.With 40X microscopic countings phagocytic rate and phagocytic index (during phagocytic rate is every 100 macrophages, Swallow the percentage shared by the macrophage of chicken red blood cell；Phagocytic index is the individual of average each macrophage phagocytic chicken red blood cell Number), and the phagocytic activity of macrophage is judged accordingly.

Phagocytic percentage=(the phagocyte number that the phagocyte number ÷ of phagocytosis chicken red blood cell is counted) × 100

The phagocyte number that the chicken red blood cell number ÷ of phagocytic index=swallowed is counted

The plant enzyme of table 6 swallows the influence (n=10) of chicken red blood cell index to peritoneal macrophage

As shown in Table 6, compare with control group, high, medium and low dosage of the invention is red thin to Turnover of Mouse Peritoneal Macrophages phagocytosis chicken The phagocytic rate of born of the same parents is respectively provided with obvious rising effect (P ＜ 0.05) with phagocytic index.Illustrating that thing of the present invention has promotes mouse to bite The phagocytosis of cell, can strengthen its non-specific immune function.

2.7NK cytoactive detections are tested.

By prescribed dose to each group test mice continuous gavage 30d after, it is aseptic to take spleen, be made individual cells suspension (cell Concentration is 2 × 107/mL), as effector cell.Take target cell (YAC-1 cells) and each 100 μ L of effector cell (are imitated target and compare 50: 1), in U-shaped 96 well culture plate of addition；Target cell Spontaneous release hole adds target cell and each 100 μ L of nutrient solution, the maximum release of target cell Hole adds each 100 μ L of target cell 2.5%Triton；Above-mentioned items are all provided with three repeating holes, in 37 DEG C, 5%CO₂Cultivated in incubator Then 96 well culture plates are centrifuged 5min by 4h with 1500r/min, per hole in the hole incubator of 100 μ L horizontalizations bottom of absorption supernatant 96, together When add the μ L of LDH matrix nights 100, react 3min, per hole add 1mol/L the μ L of HCl 30, ELIASA 490nm at measure light Density value (OD), is calculated by following equation：

NK cytoactives %=[(reaction sky OD values-Spontaneous release sky OD values)/(maximum release sky OD- Spontaneous release skies OD Value)] 100%

Influence (n=10) of the plant enzyme of table 7 to NK cells in mice activity

From table 7, compare with control group, each dosage group NK cytoactive values are above control group, and increase with dosage And increase, wherein high, middle dose group NK cytoactive values have significant difference (P ＜ 0.05 or P ＜ 0.01) compared with control group, This shows that thing of the present invention has the effect of enhancing NK cells in mice activity.

3rd, conclusion

According to《Health food is checked and assessment technique enforcement of regulations handbook》Relevant strengthen immunity examination in (2003 editions) Test result judgement standard, this experimental result：(1) plant enzyme of the invention has the effect of the function of promoting cellular immunity (small Mouse Splenic vein hemodynamics are tested positive with delayed allergy experimental result)；(2) plant enzyme of the invention has and promotes The effect (antibody-producting cell detection result of the test is positive) of the function of humoral immunity；(3) plant enzyme of the invention has and promotees Enter effect (experiment of mouse carbonic clearance and the Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment knot of monokaryon-macrophage function Fruit is positive)；(4) plant enzyme of the invention has the effect for promoting NK cytoactives.Accordingly, it is shown that plant of the invention Ferment has the effect of enhancing animal immunizing power.

Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not Can these embodiments be carried out with various changes, modification, replacement and modification in the case of departing from principle of the invention and objective, this The scope of invention is limited by claim and its equivalent.

Claims

1. it is a kind of to prepare the fermentation composition with the plant enzyme nursed one's health and strengthen immune function of human body, it is characterised in that institute Fermentation composition is stated for direct putting type plant enzyme fermenting agent, the fermentation composition includes the component of following parts by weight：Poly 0.01~10 part of 0.5~15 part of sugar, 3~22 parts of functional oligose, 0.1~5 part of probiotics and integration of drinking and medicinal herbs food materials,

Wherein, the polysaccharide is yeast beta-dextran；

The integration of drinking and medicinal herbs food materials is mainly prepared from by the raw material of following weight portion：10~45 parts of acerola concentrate powder and 3 ~26 parts of caterpillar fungus cephalosporin powder；

The particulate fine powder that the polysaccharide of predetermined weight number, functional oligose and integration of drinking and medicinal herbs food materials are made uniformly kill by mixing Bacterium, is well mixed after sterilization with probiotic powder, granulates, and the fermentation composition of powdery is obtained.

2. fermentation composition as claimed in claim 1, it is characterised in that the integration of drinking and medicinal herbs food materials extract also includes following The raw material of weight portion is extracted and formed：3~20 parts of Poria cocos, 2~18 parts of matrimony vine, 3~20 parts of mulberries, 1~10 part of white fungus, 3~20 parts of date and 2~20 parts of dandelion.

3. fermentation composition as claimed in claim 2, it is characterised in that the fermentation composition is 2~6 parts by parts by weight Yeast beta-dextran, 5~10 parts of functional oligose, 1~3 part of probiotics and 0.5~1 part of integration of drinking and medicinal herbs food materials group Into；

Wherein, the integration of drinking and medicinal herbs food materials is prepared from by the raw material of following weight portion：20~35 parts of acerola concentrate powder, 8~ 14 parts of caterpillar fungus cephalosporin powder, 9~15 parts of Poria cocos, 6~12 parts of matrimony vine, 9~15 parts of mulberries, 4~7 parts of white fungus, 6~ 12 parts of date and 9~15 parts of dandelion.

4. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition also includes that parts by weight are 10 ~30 parts of resistant dextrin.

5. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is galactooligosaccharide；

The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：45~60 parts of Bifidobacterium, 50~60 parts of Lactobacillus rhamnosus, 22~30 parts of Lactobacillus casei, 18~26 parts of lactobacillus acidophilus and Lactobacillus plantarum 23~ 30 parts, the viable bacteria content of the probiotics powder agent is 2 × 10⁶~2 × 10¹⁰cfu/g。

6. the fermentation composition as any one of claim 1-5, it is characterised in that the fermentation composition also includes weight Amount number is 1~3 part of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria；

The preparation method of the fermentation composition also includes：

(1) fermentation composition of the powdery of 1/2 amount is made softwood, is well mixed with the lactic acid bacteria of the weight portion laggard Row extrusion makes ball, is made capsule core；

(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the weight portion, is made It is standby into softwood thing；

Wherein, it by parts by weight is the adjacent benzene of Eudragit L 100-55,0.1-2 part of 0.4-10 parts that the coating solution is The aqueous dispersion that dioctyl phthalate hydroxypropyl methyl cellulose and 0.05-0.2 parts of castor oil add appropriate purified water to be configured to.

7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step Suddenly：

1) preparation of integration of drinking and medicinal herbs food materials：

By matrimony vine, mulberries, white fungus, date, the dandelion of Indian Bread and predetermined weight part with 8 times of water of weight, in temperature 80- 4h is kept at 90 DEG C, 40 DEG C are then cooled to, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering obtains the first filtrate； Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, filtering, the second filtrate；Close And filtrate, it is concentrated under reduced pressure, freeze-drying, low-temperature grinding is into meal；

2) by step 1) in fine powder mix with emulsifying agent, water after carried out with cutter 3000-5000 turn high speed shear, while stirring Mixing side dropwise addition wall material monomer carries out wall building, forms nano level particulate；

3) by the functional oligose of predetermined weight number, yeast beta-dextran, resistant dextrin and step 2) in particulate it is uniform Mixing, sterilization is carried out by heat exchanger, and condition is 99 DEG C, 300s；

4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, adds dry method to make after being well mixed Grain machine granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.

8. there is the preparation side of the plant enzyme nursed one's health and strengthen immune function of human body any one of a kind of claim 1-5 Method, it is characterised in that the described method comprises the following steps：

A () prepares powdery fermentation composition

(b) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, add 5~6L Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope Lid, lucifuge fermentation is carried out in the environment that fermentation temperature is 28~30 DEG C；

C () is fermented：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 5~8d of fermentation, filtering obtains final product plant Thing ferment.

9. there is the preparation side of the plant enzyme nursed one's health and strengthen immune function of human body any one of a kind of claim 1-5 Method, it is characterised in that the described method comprises the following steps：

A) fermentation composition of powdery is prepared

B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added The pure water of 10L, is well mixed, and after standing 1~2d of extraction, carries out high-temperature sterilization, filters, and obtains plant extraction liquid；

C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out lucifuge Fermentation 1~2 month, obtains plant enzyme stoste；

Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, gram It is several in lactobacillus, lactobacillus acidophilus and acetobacter；

D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with And plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, fermentation temperature is 28~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is constant, after Supervention ferment 3-4d, filtering, obtains final product plant enzyme.

10. there is the preparation method of the plant enzyme nursed one's health and strengthen immune function of human body, its feature described in a kind of claim 6 It is that the described method comprises the following steps：

I the fermentation composition of pellet) is prepared

II) the preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added The pure water of 10L, is well mixed, and after standing 1~2d of extraction, carries out high-temperature sterilization, filters, and obtains plant extraction liquid；

III) the preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water And plant extraction liquid 1kg, add 30g the step of II) fermentation composition of pellet for preparing, sent out in closed environment Ferment；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；After 1~2d of fermentation, insertion conduit is passed through air, and ventilate 2-4 daily It is secondary, each 10-30min, and it is 35~37 DEG C to control fermentation temperature；Filtered after 5~7d of fermentation, obtain final product plant enzyme.