CN106858579A - Prepare the fermentation composition and preparation method of the plant enzyme for being applied to diabetes conditioning and routine servicing - Google Patents

Prepare the fermentation composition and preparation method of the plant enzyme for being applied to diabetes conditioning and routine servicing Download PDF

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CN106858579A
CN106858579A CN201710234608.4A CN201710234608A CN106858579A CN 106858579 A CN106858579 A CN 106858579A CN 201710234608 A CN201710234608 A CN 201710234608A CN 106858579 A CN106858579 A CN 106858579A
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fermentation
plant
fermentation composition
lactobacillus
parts
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姜红成
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention provides the fermentation composition for preparing the plant enzyme suitable for diabetes conditioning and routine servicing, it is made up of functional oligose, probiotics and Chinese medical extract；Chinese medical extract is extracted by the raw material of following weight portion and formed：0.3~2 part of the stem of noble dendrobium, 0.3~2 part of matrimony vine, 0.2~3 part of balsam pear, 0.5~3 part of the root of kudzu vine, 0.3~2 part of sealwort, 0.5~3 part of cassia seed, 0.9~3 part of Chinese yam, 0.1~1.8 part of Radix Glycyrrhizae, 0.1~1.8 part of mulberry leaf and 0.3~2 part of lily.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", the industrialized production of suitable all kinds of plant enzymes.

Description

Prepare the fermentation composition of the plant enzyme suitable for diabetes conditioning and routine servicing And preparation method
Technical field
The invention belongs to prevent conditioning maintenance product field, more particularly to one kind is prepared and nursed one's health suitable for diabetes and daily foster The fermentation composition and preparation method of the plant enzyme of shield.
Background technology
Plant enzyme is always the best seller of ferment class product.Plant enzyme is by tens of kinds of different vegetables and fruits, cereal, seas The plant such as algae and mushroom class, develops through spontaneous fermentation, remains the nutrition elite in plant, contains the various of human body needs Ferment, oligosaccharides and various vitamins and mineral matter, can produce abundant SOD antioxidase compositions again, improve anti-oxidant energy in vivo Power, and then strengthen immunity.
To make enzyme food more conform to modern to the diversity of nutritional need, balance and being easy to absorbability, no matter It is that traditional handicraft or modern crafts still need further improvement.Existing traditional handicraft and modern crafts to ferment is all entered One step is improved, while also occurring in that health food prepared by the ferment and ferment of some addition probiotics.Probiotics fermention is really Vegetable, can activate the effective efficiency composition in fruits and vegetables, active effect material of dissolution more elite.But traditional ferment technique cannot Flexibly use on a large scale, it is impossible to convenient in the industrial manufacturing processes of difference in functionality ferment to use；Promoting to improve ferment Enter the effect of the aspects such as internal metabolism, coordinating intestines and stomach function, and adaptation is used on a large scale, applicant is to preparing ferment Fermentate further improve, and develop improve ferment function affect formula.
Additionally, functional oligose is a class have physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degrade, not small intestine inhale Receive, the oligosaccharide of big intestines can be reached；Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, and with promoting, human body is prebiotic The physiological functions such as the propagation of bacterium.It is used in conjunction with by by probiotics and functional oligose, between the two with synergy, energy Promote the regulatory function to gut flora.Co between probiotics and oligosaccharide, be probiotic in symphysis unit and Co between prebiotics, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria Growth；Research shows that both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no This effect.
Based on above-mentioned technology, the artificial feature for improving ferment of application is added with the ferment fermentate of prior art The traditional Chinese medicine ingredients of effect, the effective regulating intestinal canal flora of ferment for enabling it to prepare, strengthen immunity, and with function.
On the other hand, existing ferment bucket, prepares plant enzyme so that plant enzyme by raw material of fruit, sugar and water Can be prepared in family, it is easy to operate.But also there are many weak points, the time that 1. prepared by ferment is still more long, Er Qierong Easily prepare failure；2. plant enzyme effect is indefinite obtained in, user cannot oneself control prepare effect of plant enzyme, than Such as nurse one's health the plant enzyme of diabetes；3. the low molecular sugar and polysaccharide majority in the plant such as fruit cannot be utilized, it is impossible to be converted into The oligosaccharide of given efficacy.For the deficiency, applicant puts forth effort research has shortening ferment preparation time, easy to use and energy Nurse one's health the fermentation composition of diabetes, i.e. ferment bag.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention It is to propose a kind of fermentation composition for preparing the plant enzyme suitable for diabetes conditioning and routine servicing, the fermentation composition It is directly putting type fermented microbial inoculum, probiotics and functional oligose is used in combination, while adds traditional Chinese medicine ingredients.Use the fementative composition Plant enzyme prepared by thing plays effect of conditioning diabetes, and has functions that routine servicing to diabetic population.
Technical scheme is as follows：
A kind of fermentation composition for preparing the plant enzyme suitable for diabetes conditioning and routine servicing, the fementative composition Thing is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight：Functional oligose 3~ 0.01~10 part of 22 parts, 0.1~5 part of probiotics and Chinese medical extract,
Wherein, the probiotics is selected from one or more in Bifidobacterium, lactobacillus and streptococcus thermophilus；
The Chinese medical extract is extracted by the raw material of following weight portion and formed：0.3~2 part of the stem of noble dendrobium, 0.3~2 part of Chinese holly Qi, 0.2~3 part of balsam pear, 0.5~3 part of the root of kudzu vine, 0.3~2 part of sealwort, 0.5~3 part of cassia seed, 0.9~3 part of mountain Medicine, 0.1~1.8 part of Radix Glycyrrhizae, 0.1~1.8 part of mulberry leaf and 0.3~2 part of lily；
The preparation method of the fermentation composition at least comprises the steps：
It is sterilized after the fine powder mixing that the functional oligose and Chinese medical extract of predetermined weight number are made, after sterilization with Probiotics powder agent uniformly mixes, granulation, and the fermentation composition of powdery is obtained.
Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity；And the traditional Chinese medicine ingredients added, enter One step realizes prevention or treatment diabetes effect.And fermentation composition is made powdery, the later stage is easy in the production of implementation phase formula Direct plunge into use or family oriented is used.
Further, the fermentation composition is by functional oligose that parts by weight are 5~10 parts, 1~3 part prebiotic Bacterium and 0.5~1 part of Chinese medical extract composition.
Further, Chinese medical extract is extracted by the raw material of following weight portion and formed：0.9~1.2 part of the stem of noble dendrobium, 0.9~ 1.2 parts of matrimony vine, 0.6~1.5 part of balsam pear, 1.2~1.5 parts of the root of kudzu vine, 1~1.5 part of sealwort, 1~1.5 part of Cassia Son, 1.5~2 parts of Chinese yam, 0.3~0.9 part of Radix Glycyrrhizae, 0.5~0.9 part of mulberry leaf and 0.9~1.2 part of lily.
In such scheme, the functional oligose is selected from shitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew Oligosaccharide, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar, One or more in trehalose, palatinose and inulin；
Bifidobacterium includes bifidobacterium longum (B.longum), short Bifidobacterium Bifidum (B.breve) (B.breve), bifidobacterium infantis (B.infantis) one or more and in bifidobacterium lactis (B.ifidobacterium)；
Lactobacillus includes Lactobacillus rhamnosus (L.rhamnosus), Lactobacillus casei (Lactobacillus Casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus plantarum), saliva breast bar Bacterium (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus helveticus), Bu Shi lactobacillus (Lactobacillus buchneri), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), secondary cheese breast bar One kind or several in bacterium (Lactobacillus paracasei) and lactobacillus reuteri (Lactobacillus reuteri) Kind.
The preferred scheme of fermentation composition of the invention, also including the resistant dextrin that parts by weight are 10~30 parts, resistance Dextrin is used in conjunction with other components, further promotes intestines peristalsis, keeps intestinal health, and control the absorption of sugar.
Further, the functional oligose is kanjak mannan-oligosaccharides；
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：Bifidobacterium 45~ 23~30 parts of 60 parts, 20~25 parts of lactobacillus bulgaricus, 14~18 parts of lactobacillus acidophilus and Lactobacillus plantarum, it is described prebiotic The viable bacteria content of bacteria powder is 2 × 108~8 × 108cfu/g；By all kinds of strains of reasonable disposition and consumption, optimization this patent is produced The strain of quality structure, can effectively shorten fermentation composition in the time for preparing plant enzyme, and it can be made to prepare texture stabilization Plant enzyme.
The source of above-mentioned all kinds of probiotics powder agents：It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of Strain is collected by centrifugation with after washing thalline through Liquid Culture, and drying is prepared into active powder.
In another inventive technique scheme, fermentation composition of the invention is also including yeast that parts by weight are 1~3 part Bacterium, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria；
The preparation method of the fermentation composition also includes：
(1) fermentation composition of the powdery of 1/2 amount is made softwood, the lactic acid bacteria with the weight portion is well mixed After carry out extrusion and make ball, be made capsule core；
(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the weight portion It is even, softwood thing is made, it is standby；
(3) obtained capsule core is placed in the coating solution of advance preparation, is coated；
(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product Pellet fermentation composition；
Wherein, it by parts by weight is 0.4-10 parts of acroleic acid resin I I, 0.1-2 parts of adjacent benzene that the coating solution is Dicarboxylate adds the aqueous dispersion that appropriate purified water is configured to.
As above, the fermentation pill with double-layer structural, outer layer fermentation ball mixing composite bacteria -- saccharomycete and vinegar are prepared into Acidfast bacilli, internal layer fermentation ball is mixed with lactic acid bacteria, the step of eliminate later stage multistage inoculation composite bacteria, improves what product was used Convenience；And by coating membrane separate between ectonexine and can effectively extend the time limit that internal layer pill starts dissolving, reach two Level inoculation fermentation effect, realizes deep layer liquid state fermentation.
The preparation method of the fermentation composition of powdery of the present invention, comprises the following steps：
1) preparation of Chinese medical extract：
The root of kudzu vine of the weight portion, sealwort and cassia seed are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder, sealwort powder is obtained End and Cassia Seed；
By root of kudzu vine powder, Solomonseal Rhizome, the stem of noble dendrobium of Cassia Seed and predetermined weight part, matrimony vine, balsam pear, Chinese yam, Radix Glycyrrhizae, Mulberry leaf, lily keep 4h with 8 times of water of weight at 80-90 DEG C of temperature, are then cooled to 40 DEG C, plus 2 times of weight concentrations are 75% alcohol reflux 4h, filtering, obtains the first filtrate；Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, Then be cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, freeze-drying, and low-temperature grinding is into being ground into The traditional Chinese medicine fine powder of 0.05-0.2mm；
2) by step 1) in traditional Chinese medicine fine powder mix with emulsifying agent, water after carry out high speed shear with cutter, form particle Chinese medicine particulate of the particle diameter less than 50 microns；
3) by the functional oligose of predetermined weight number, resistant dextrin and step 2) in Chinese medicine particulate uniformly mix, Sterilization is carried out by heat exchanger, condition is 99 DEG C, 300s；
4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, is added after being well mixed dry Method granulator granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.
A further object of the present invention be to provide it is a kind of suitable for diabetes conditioning and routine servicing plant enzyme, be pin Personal or Homemade plant enzyme is formulated, the preparation method of the plant enzyme is comprised the following steps：
A () prepares powdery fermentation composition
(b) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, add 5 The pure water of~6L, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket Interior, capping carries out lucifuge fermentation in the environment that fermentation temperature is 28~30 DEG C；
C () is fermented：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 5~8d of fermentation, filtering, i.e., Obtain plant enzyme.
The method simple and fast, realizes the self-control of various functions plant enzyme, and the method preferably uses ferment bucket and made Standby, ferment bucket is from market sale；Also the vessel of closed environment are can be used to be prepared.
Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps：
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added Enter the pure water of 10L, be well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filter, obtain plant extraction liquid；
C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 1~2 month, obtains plant enzyme stoste；
Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, leather It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter；
D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is not Become, continue the 3-4d that ferments, filtering obtains final product plant enzyme.
Which is applied to industrial production, secondary with fermentation composition of the present invention with reference to plant enzyme stoste first fermentation Multistage deep layer liquid state fermentation is realized in fermentation；Discharge and be enclosed in Chinese medicine or plant structure and intracellular nutrients element, to plant Original active material is retained, and insoluble fibre is changed into effect that soluble dietary fiber improves original plant in plant.
The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition for also being constructed for double-deck pellet Method, the preparation method is comprised the following steps：
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and The pure water of 10L is added, is well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filtered, obtain plant extraction liquid；
III) the preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water and plant extraction liquid 1kg, add 30g the step of II) fermentation composition of pellet for preparing, carried out in closed environment Fermentation；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；After 1~2d of fermentation, insertion conduit is passed through air, daily ventilation 2-4 times, each 10-30min, and it is 35~37 DEG C to control fermentation temperature；Filtered after 5~7d of fermentation, obtain final product plant enzyme.
The method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation Realize second order fermentation, or even multistage fermentation；The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries Its effect high.
The present invention has the following advantages that compared with prior art：
The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly with the bacterium bag of pulvis in city Sold on face, can also put into the industrialized production of all kinds of plant enzymes, realize matching somebody with somebody i.e. with the work(with diversification for plant enzyme Energy property configuration, is greatly enhanced the preparation efficiency of plant enzyme.
The traditional Chinese medicinal components added in fermentation composition of the invention have its physiologically active and health-care effect, while being prebiotic Bacterium supplements the nutrients in fermentation, and with practical value higher, and blood high can effectively be prevented or be treated to traditional Chinese medicine ingredients of the invention Sugared disease；Compatibility is reasonable between probiotics, functional oligose and Chinese medicine each component, clear curative effect, with Synergistic Effect.
Plant enzyme of the invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body The comprehensive plant enzyme of benefit, these enzymes play an important role to the metabolism of human body and function adjustment, while also containing vitamin, mineral The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group Traditional Chinese medicine ingredients in compound are changed through probiotics, and small molecule is become by macromolecular, are easily absorbed by the body；The addition of probiotics can Improve Chinese medicine taste, toxicity can be decomposed in fermentation process, realize it is real have no toxic side effect, while the fermentation composition prepare Plant enzyme improves drug effect compared to traditional Chinese medicine, improves glycometabolism, regulating intestinal canal flora.The suitable hyperglycaemia of plant ferment Crowd is used, and is taken as daily drinks, and lipid-metabolism is improved from the angle effected a permanent cure, and improves body immunity etc., realizes health care Purpose.
Additionally, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.
The source of Chinese medicine material composition of the invention：
The stem of noble dendrobium：This product is orchid dendrobium loddigesii Rolfe Dendrobium loddigesii Rolfe., stem of Eyeshaped Dendrobium Dendrobium fimbriatum Hook.var.oculatum Hook., HERBA DENDROBII Dendrobium chrysanthum Wall., dendrobium candidum Dendrobium candidum Wall.ex Lindl. or HERBA DENDROBII Dendrobium nobile Lindl. fresh dries stem.
Matrimony vine：This product is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..
Balsam pear：Curcurbitaceae balsam pear platymiscium balsam pear Momordica charantia L., are used as medicine with melon, root, rattan and leaf.
The root of kudzu vine：This product is legume pueraria lobata Pueraria lobata (Willd.) Ohwi or sweet kudzu Pueraria The dry root of thomsonii Benth..
Sealwort：This product is liliaceous plant P. kingianum Polygonatum kingianum Coll.et Hemsl., sealwort The dry rhizome of Polygonatum sibiricum Red. or David's-harp Polygonatum cyrtonema Hua.
Cassia seed：This product is legume Cassia Cassia obtusifolia L. or little Cassia tora Cassia tora L. Dry mature seed.
Chinese yam：This product is the dry rhizome of Dioscoreaceae plant Chinese yam Dioscorea opposita Thunb..
Radix Glycyrrhizae：This product is glycyrrhizic legume Glycyrrhiza uralensis Fi sch., swollen fruit Radix The dry root of Glycyrrhiza inflata Bat. or glycyrrhiza glabra Glycyrrhiza glabra L..
Mulberry leaf：This product is the dried leaf of moraceae plants mulberry Morus alba L..
Lily：This product is liliaceous plant tiger lily Lilium lancifolium Thunb., lily Lilium brownii The dry meat scale leaf of F.E.Brown var.viridulum Baker or Lilium tenuifolium Lilium pumilum DC..
Specific embodiment
The fermentation composition of embodiment 1
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Kanjak mannan-oligosaccharides 80g
Probiotics powder agent：24g, wherein containing 4.5 parts of Bifidobacterium, 2 parts of lactobacillus bulgaricus, thermophilic in per g probiotic powders 2.3 parts of 1.8 parts of Lactobacillus lactis and Lactobacillus plantarum；
Traditional Chinese medicine ingredients：8g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：1.2 parts of the stem of noble dendrobium, 1.2 parts of matrimony vine, 1.5 parts of balsam pear, 1.5 parts of the root of kudzu vine, 1.5 parts of sealwort, 1.5 parts of cassia seed, 2 parts of Chinese yam, 0.9 part of Radix Glycyrrhizae, mulberry leaf 0.9 1.2 parts of part and lily；
Resistant dextrin 80g.
Preparation method：
1) preparation of Chinese medical extract：
The root of kudzu vine of the weight portion, sealwort and cassia seed are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder, sealwort powder is obtained End and Cassia Seed；
By root of kudzu vine powder, Solomonseal Rhizome, the stem of noble dendrobium of Cassia Seed and predetermined weight part, matrimony vine, balsam pear, Chinese yam, Radix Glycyrrhizae, Mulberry leaf, lily keep 4h with 8 times of water of weight at 80-90 DEG C of temperature, are then cooled to 40 DEG C, plus 2 times of weight concentrations are 75% alcohol reflux 4h, filtering, obtains the first filtrate；Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, Then be cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, freeze-drying, and low-temperature grinding is into being ground into The traditional Chinese medicine fine powder of 0.05-0.2mm；
2) by step 1) in traditional Chinese medicine fine powder mix with emulsifying agent, water after carry out high speed shear with cutter, form particle Chinese medicine particulate of the particle diameter less than 50 microns；
3) by the functional oligose of predetermined weight number, resistant dextrin and step 2) in Chinese medicine particulate uniformly mix, Sterilization is carried out by heat exchanger, condition is 99 DEG C, 300s；
4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, is added after being well mixed dry Method granulator granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.
The fermentation composition of embodiment 2
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Kanjak mannan-oligosaccharides 50g
Probiotics powder agent：10g, wherein containing 6 parts of Bifidobacterium, 2.5 parts of lactobacillus bulgaricus, thermophilic in per g probiotic powders 3 parts of 1.4 parts of Lactobacillus lactis and Lactobacillus plantarum；
Traditional Chinese medicine ingredients：5g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：0.9 part of the stem of noble dendrobium, 0.9 part of matrimony vine, 0.6 part of balsam pear, 1.2 parts of the root of kudzu vine, 1 part of sealwort, 1 part of cassia seed, 1.5 parts of Chinese yam, 0.3 part of Radix Glycyrrhizae, 0.5 part of mulberry leaf And 0.9 part of lily；
Preparation method：
1) preparation of Chinese medical extract：
The root of kudzu vine of the weight portion, sealwort and cassia seed are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder, sealwort powder is obtained End and Cassia Seed；
By root of kudzu vine powder, Solomonseal Rhizome, the stem of noble dendrobium of Cassia Seed and predetermined weight part, matrimony vine, balsam pear, Chinese yam, Radix Glycyrrhizae, Mulberry leaf, lily keep 4h with 8 times of water of weight at 80-90 DEG C of temperature, are then cooled to 40 DEG C, plus 2 times of weight concentrations are 75% alcohol reflux 4h, filtering, obtains the first filtrate；Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, Then be cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, freeze-drying, and low-temperature grinding is into being ground into The traditional Chinese medicine fine powder of 0.05-0.2mm；
2) by step 1) in traditional Chinese medicine fine powder mix with emulsifying agent, water after carry out high speed shear with cutter, form particle Chinese medicine particulate of the particle diameter less than 50 microns；
3) by the functional oligose and step 2 of predetermined weight number) in Chinese medicine particulate uniformly mix, by heat exchanger Sterilization is carried out, condition is 99 DEG C, 300s；
4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, is added after being well mixed dry Method granulator granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.
The fermentation composition of embodiment 3
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Kanjak mannan-oligosaccharides 60g
Probiotics powder agent：2g, wherein containing 5.5 parts of Bifidobacterium, 2.2 parts of lactobacillus bulgaricus, thermophilic in per g probiotic powders 2.5 parts of 1.5 parts of Lactobacillus lactis and Lactobacillus plantarum；
Traditional Chinese medicine ingredients：0.2g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：The stem of noble dendrobium 0.3 Part, 0.3 part of matrimony vine, 0.2 part of balsam pear, 0.5 part of the root of kudzu vine, 0.3 part of sealwort, 0.5 part of cassia seed, 0.9 part of Chinese yam, 0.1 part of Radix Glycyrrhizae, mulberry 0.3 part of 0.1 part of leaf and lily；Resistant dextrin 200g
Preparation method：Prepared by the preparation method according to embodiment 1, pulvis, every bag of 30g is obtained.
The fermentation composition of embodiment 4
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Konjac oligosaccharides 120g, inulin 100g；
Probiotics powder agent：50g, wherein in per g probiotic powders containing 5.6 parts of Bifidobacterium, 2.4 parts of lactobacillus bulgaricus, 2.8 parts of 1.6 parts of lactobacillus acidophilus and Lactobacillus plantarum；
Traditional Chinese medicine ingredients：100g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：2 parts of the stem of noble dendrobium, 2 parts of matrimony vine, 3 parts of balsam pear, 3 parts of the root of kudzu vine, 2 parts of sealwort, 3 parts of cassia seed, 3 parts of Chinese yam, 1.8 parts of Radix Glycyrrhizae, 1.8 parts of mulberry leaf and lily 2 parts.
Preparation method：Prepared by the preparation method according to embodiment 2, pulvis, every bag of 30g is obtained.
The fermentation composition of embodiment 5
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Xylo-oligosaccharide 83g soy oligosaccharides Icing Sugar 61g FOSs 56g
Probiotics powder agent：20g, wherein containing 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva in per g probiotic powders 2.2 parts of 0.9 part of lactobacillus, 1.5 parts of lactobacillus acidophilus and Lactobacillus plantarum；
Traditional Chinese medicine ingredients：50g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：1.5 parts of the stem of noble dendrobium, 1.5 parts of matrimony vine, 1.8 parts of balsam pear, 2.1 parts of the root of kudzu vine, 1 part of sealwort, 1.6 parts of cassia seed, 2.3 parts of Chinese yam, 0.6 part of Radix Glycyrrhizae, mulberry leaf 0.7 Part and 1.3 parts of resistant dextrin 300g of lily
Preparation method：Prepared by the preparation method according to embodiment 1, pulvis, every bag of 30g is obtained.
The fermentation composition of embodiment 6
The prescription of the fermentation composition of the present embodiment is：
Functional oligose：Xylo-oligosaccharide 43g, mannan-oligosaccharides 40g, FOS 23g, galactooligosaccharide 22g and Trehalose 19g；
Probiotics powder agent：10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, mouse in per g probiotic powders 2.4 parts of lactobacillus of Lee's sugar, 1.3 parts of Lactobacillus helveticus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and secondary cheese breast 0.9 part of bacillus；
Traditional Chinese medicine ingredients：4.55g, wherein extracted by the raw material of following portions by weight per g Chinese medical extracts forming：The stem of noble dendrobium 0.6 Part, 1.7 parts of matrimony vine, 2.3 parts of balsam pear, 2 parts of the root of kudzu vine, 0.7 part of sealwort, 2.2 parts of cassia seed, 1.8 parts of Chinese yam, 0.5 part of Radix Glycyrrhizae, mulberry leaf 0.4 and 1.2 parts of lily；Resistant dextrin 70.5g
Preparation method：Prepared by the preparation method according to embodiment 1, pulvis, every bag of 30g is obtained.
The fermentation composition of embodiment 7
The prescription of the fermentation composition of the present embodiment is：
The pulvis fermentation composition 32g that embodiment 1 is made；Saccharomycete 1g；Acetobacter 1g；Lactic acid bacteria 1g；Coating solution by The appropriate purifying of acroleic acid resin II 20g, diethyl phthalate 5g addition is prepared；
Preparation method：
(1) the pulvis fermentation composition of 1/2 amount is made softwood, after being well mixed with the lactic acid bacteria of the weight portion Carry out extrusion and make ball, be made capsule core；
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the weight portion, Softwood thing is made, it is standby；
(3) obtained capsule core is placed in the coating solution of advance preparation, is coated；
(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core；It is preferred that pellet diameters 5mm, capsule core is a diameter of 2.8mm。
The fermentation composition of embodiment 8
The prescription of the fermentation composition of the present embodiment is：
The pulvis fermentation composition 23.1g that embodiment 3 is made；Saccharomycete 3g；Acetobacter 3g；Lactic acid bacteria 5g；Coating solution Prepared by the appropriate purifying of acroleic acid resin II 10g, diethyl phthalate 2g addition；
Preparation method：
(2) the pulvis fermentation composition of 1/2 amount is made softwood, after being well mixed with the lactic acid bacteria of the weight portion Carry out extrusion and make ball, be made capsule core；
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the weight portion, Softwood thing is made, it is standby；
(3) obtained capsule core is placed in the coating solution of advance preparation, is coated；
(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core；It is preferred that pellet diameters 5mm, capsule core is a diameter of 2.8mm。
The plant enzyme of embodiment 9
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition:It is prepared by the fermentation composition according to embodiment 1；
B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added Enter the pure water of 10L, be well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filter, obtain plant extraction liquid；
C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 2 months, obtains plant enzyme stoste；
Wherein inoculum concentration is 12%；Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid In bacillus；
D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is not Become, continue the 3-4d that ferments, filtering obtains final product plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g, and clean section is used.
Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
The plant enzyme of embodiment 10
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 2；
B) preparation of plant extraction liquid：The plant material of 1kg is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and add 5~6L pure water, be well mixed, stand extraction 1~2d after, carry out high-temperature sterilization, filter, obtain plant extract Liquid；
C) mixing and initial fermentation：Plant in fermentation composition to step b) prepared by the step of addition weight is 60g a) In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, obtains final product plant enzyme.
Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip 140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.
Organoleptic parameters：The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant Gas, mellow in taste, comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
The plant enzyme of embodiment 11
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 3；
B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added Enter the pure water of 10L, be well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filter, obtain plant extraction liquid；
C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 1 month, obtains plant enzyme stoste；
Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, leather It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter；
D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is not Become, continue the 3-4d that ferments, filtering obtains final product plant enzyme.
Wherein, plant material selects passion fruit 120g, oranges and tangerines 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery 40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.
Organoleptic parameters：The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
The plant enzyme of embodiment 12
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition：It is prepared by the fermentation composition according to embodiment 5；
B) preparation of plant extraction liquid：The plant material of 1kg is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and add 5~6L pure water, be well mixed, stand extraction 1~2d after, carry out high-temperature sterilization, filter, obtain plant extract Liquid；
C) mixing and initial fermentation：Fermentation composition prepared by the step of addition weight is 30~60g a) is in step b) Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, obtains final product plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
The plant enzyme of embodiment 13
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition:It is prepared by the fermentation composition according to embodiment 1；
B) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, addition 5~ The pure water of 6L, is well mixed, while add in fermentation composition to the ferment bucket that the step of weight is 20~50g prepared by a), Capping, lucifuge fermentation is carried out in the environment that fermentation temperature is 28~30 DEG C；
C) ferment：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 4~6d of fermentation, filtering is obtained final product Plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
The plant enzyme of embodiment 14
The plant enzyme of the present embodiment is prepared from by following methods：
I the fermentation composition of pellet) is prepared according to embodiment 7
II) the preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and The pure water of 10L is added, is well mixed, after standing 1~2d of extraction, carry out high-temperature sterilization, filtered, obtain plant extraction liquid；
III) the preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water and plant extraction liquid 1kg, add 30g the step of II) fermentation composition of pellet for preparing, carried out in closed environment Fermentation；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；After 1~2d of fermentation, insertion conduit is passed through air, daily ventilation 2-4 times, each 10-30min, and it is 35~37 DEG C to control fermentation temperature；Filtered after 5~7d of fermentation, obtain final product plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters：The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste, Comfortable acid, entrance does not stimulate, and pleasant impression is sweet.
Observation to the pellet fermentation composition fermentation process of embodiment 7：Pellet fermentation composition during the fermentation, ball The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten Solution.
The fermentation composition of comparative examples 1
The prescription of the fermentation composition of the present embodiment is：：
Functional oligose：Kanjak mannan-oligosaccharides 80g
Probiotics powder agent：24g, wherein containing 4.5 parts of Bifidobacterium, 2 parts of lactobacillus bulgaricus, thermophilic in per g probiotic powders 2.3 parts of 1.8 parts of Lactobacillus lactis and Lactobacillus plantarum；
Resistant dextrin 80g.
Preparation method：
The functional oligose of predetermined weight number, probiotics powder agent, resistant dextrin are uniformly mixed into 10~15min, will Mixed powder adds dry granulating machine granulation, and the pulvis of fermentation composition, pack, every bag of 30g is obtained.
The plant enzyme of comparative examples 2
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of fermentation composition：Prepared by the fermentation composition of comparative examples 1；
B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added Enter the pure water of 10L, be well mixed, after standing extraction 2d, carry out high-temperature sterilization, filter, obtain plant extraction liquid；
C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out Lucifuge is fermented 1~2 month, obtains plant enzyme stoste；
Wherein inoculum concentration is 12%；Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid In bacillus；
D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L Water and plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, and fermentation temperature is 28 ~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is not Become, continue the 3-4d that ferments, filtering obtains final product plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
The plant enzyme of comparative examples 3
The plant enzyme of the present embodiment is prepared from by following methods：
A) preparation of plant extraction liquid：The plant material of 1kg is cleaned, the sugared to plant material of 0.3~0.6kg weight is added In, and add 5~6L pure water, be well mixed, stand extraction 1~2d after, carry out high-temperature sterilization, filter, obtain plant extract Liquid；
B) mixing and initial fermentation：In plant extraction liquid in inoculation compound probiotic to step b), mixing is placed in closed Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid；
C) zymotic fluid carries out after-ripening 7d in closed environment, takes the filtering of Partial fermentation liquid, does organoleptic parameters；Continue after-ripening 14d, takes the filtering of Partial fermentation liquid, does organoleptic parameters.
Wherein, compound probiotic is Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus and plant Lactobacillus；Inoculum concentration is 8% (mass percent)；
Plant material selects apple 500g, soybean 200g and tomato 300g.
The organoleptic parameters of the zymotic fluid after 7 days：The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has Stimulation, sweet taste are heavier；The organoleptic parameters of the zymotic fluid after 14 days：The plant enzyme uniform color, in bronzing, there is strong Fruity does not stimulate with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method The fermentation time that ferment needs is longer than the fermentation time of plant enzyme of the present invention.
The viable bacteria survival detection of the fermentation composition of test example 1
Fermentation composition prepared by the embodiment of the present invention 1 and embodiment 7, with sterilized water be prepared into viable count for 2 × 108The solution to be measured of CFU/mL, saves backup in 4 DEG C；
The solution to be measured of the 10mL for keeping of going bail for is injected into test tube 1, using ten times of stepwise dilutions to 10-8, take 1mL dilutions Liquid will be cooled to 45 DEG C of MRS agar mediums and pour on flat board (sterilizing) on flat board after sterilizing, shake up rapidly.Again will The test tube 2 of the solution to be measured equipped with 10mL is placed in 80-90 DEG C of water-bath and heats 15-25min, takes the solution to be measured after heating and enters Ten times of stepwise dilutions of row are to 10-8, 1mL dilutions are taken on flat board, the MRS agar mediums that 45 DEG C will be cooled to after sterilizing are poured into Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates Number of viable before and after heating.
Result shows that Viable detection is respectively 89% and 90.5%.
The viable bacteria survival detection of the plant enzyme of test example 2
1st, the resistance test of SGF and intestinal juice
The hydrochloric acid 16.4mL plus distilled water diluting of 100g/L are taken, pH value is respectively 1.5,2.5 and 3.5, take the dilute salt of 100mL Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains SGF, and miillpore filter degerming (0.22 μm) is standby.Take Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value to 6.8 is adjusted with 0.1moL/L sodium hydroxide solutions；Separately take tryptose Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, and miillpore filter is degerming (0.22 μm) is standby.
Take embodiment 9 that 1mL keeps and the plant enzyme solution of embodiment 14 is added to (i.e. ten in the SGF of 9mL Times stepwise dilution), and it is rapid fully mix on the oscillator, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively 1h, 2h, Nutrient solution is taken out when 3h, 4h and remaining viable count is counted immediately, is compared with former viable count, as a result shown, viable bacteria is deposited Motility rate is respectively 98.1% and 97.4%.Then each 1mL of nutrient solution that different time is digested in simulated gastric fluid is taken, is inoculated with respectively In the simulated intestinal fluid that 9mL pH value is 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h samplings, survey Fixed its viable count, is compared with former viable count, as a result shows that Viable detection is respectively 98.2% and 97.6%.
The plant enzyme of test example 3 nurses one's health diabetes evaluation
Foundation《Health food is examined and assessment technique specification》Auxiliary hyperglycemic function human trial evaluation method to implement The plant enzyme that example 9, embodiment 13, embodiment 14, comparative examples 2 are prepared is evaluated, specific as follows：
1st, evaluation method：
According to 44 type II diabetes people, the wherein people of male 26, the people of women 18, the range of age 38-75 Sui, without serious conscience The complication such as kidney, in taking before its test-meal after Data Comparison.
Subject is randomly divided into 4 groups, every group of 11 people, subject's original diet control and activity are constant, and packet situation is：
Group 1：The plant enzyme of edible embodiment 9；
Group 2：The plant enzyme of edible embodiment 13；
Group 3：The plant enzyme of edible embodiment 14；
Group 4：The plant enzyme of edible comparative examples 2；
It is taken twice daily, each 60ml, is taken after being diluted with warm water.
Monitoring patient blood pressure, stool and urine, the change of body weight, and the blood sugar determined on an empty stomach with 2 hours after the meal (uses grape glycosyloxy Chemical-enzyme method).
2nd, effect criterion reference：
It is effective：Cardinal symptom disappears, and declines on an empty stomach and before the relatively treatment of the blood sugar of 2 hours after the meal and is no more than 30%；
Effectively：Cardinal symptom is obviously improved, and declines no more than 10% on an empty stomach and before the blood sugar of 2 hours is relatively treated after the meal；
It is invalid：Cardinal symptom is not improved, and the blood sugar of 2 hours is dropped by less than 10% before relatively treating on an empty stomach and after the meal.
3rd, evaluation result：
Compared with before test-meal, after test-meal 30 days, blood pressure, stool and urine, the body weight of the patient of each group ferment are taken without aobvious Sex differernce is write, but fasting blood-glucose, after the meal 2 hours blood glucoses have substantially reduction, and concrete outcome is shown in Table 1.
The blood glucose test results of the test-meal sufferer of table 1
From the above results, the plant enzyme blood sugar decreasing effect of group 1, group 2 and group 3 is more notable, wherein organizing 3 effects most For notable；Plant enzyme effect prepared by the no added traditional Chinese medicine ingredients fermentation composition of group 4 is worst.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not Can these embodiments be carried out with various changes, modification, replacement and modification in the case of departing from principle of the invention and objective, this The scope of invention is limited by claim and its equivalent.

Claims (10)

1. it is a kind of prepare suitable for diabetes conditioning and routine servicing plant enzyme fermentation composition, it is characterised in that institute Fermentation composition is stated for direct putting type plant enzyme fermenting agent, the fermentation composition includes the component of following parts by weight：Function Property 0.01~10 part of 3~22 parts of oligosaccharide, 0.1~5 part of probiotics and Chinese medical extract,
Wherein, the probiotics is selected from the jelly of one or more in Bifidobacterium, lactobacillus and streptococcus thermophilus Dry powder；
The Chinese medical extract is extracted by the raw material of following weight portion and formed：0.3~2 part of the stem of noble dendrobium, 0.3~2 part of matrimony vine, 0.2~3 part of balsam pear, 0.5~3 part of the root of kudzu vine, 0.3~2 part of sealwort, 0.5~3 part of cassia seed, 0.9~3 part of Chinese yam, 0.1~1.8 part of Radix Glycyrrhizae, 0.1~1.8 part of mulberry leaf and 0.3~2 part of lily；
The preparation method of the fermentation composition at least comprises the steps：
It is sterilized after the fine powder mixing that the functional oligose and Chinese medical extract of predetermined weight number are made, after sterilization with it is prebiotic Bacteria powder uniformly mixes, granulation, and the fermentation composition of powdery is obtained.
2. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition is 5~10 by parts by weight Part functional oligose, the Chinese medical extract composition of 1~3 part of probiotics and 0.5~1 part；
The Chinese medical extract is extracted by the raw material of following weight portion and formed：0.9~1.2 part of the stem of noble dendrobium, 0.9~1.2 part of Chinese holly Qi, 0.6~1.5 part of balsam pear, 1.2~1.5 parts of the root of kudzu vine, 1~1.5 part of sealwort, 1~1.5 part of cassia seed, 1.5~2 parts Chinese yam, 0.3~0.9 part of Radix Glycyrrhizae, 0.5~0.9 part of mulberry leaf and 0.9~1.2 part of lily.
3. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is selected from shitosan, low Xylan, kanjak mannan-oligosaccharides, mannan-oligosaccharides, milk ketose, raffinose, soyabean oligosaccharides, FOS, oligomeric gala One or more in sugar, lactosucrose, konjac mannan sugar, trehalose, palatinose and inulin；
The Bifidobacterium includes the one kind in bifidobacterium longum, bifidobacterium breve, bifidobacterium infantis and bifidobacterium lactis Or it is several；
The lactobacillus include Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus salivarius, One kind or several in Lactobacillus helveticus, Bu Shi lactobacillus, lactobacillus bulgaricus, lactobacillus paracasei and lactobacillus reuteri Kind.
4. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition also includes that parts by weight are 10 ~30 parts of resistant dextrin.
5. fermentation composition as claimed in claim 4, it is characterised in that the functional oligose is that kanjak mannan is oligomeric Sugar；
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight：45~60 parts of Bifidobacterium, 23~30 parts of 20~25 parts of lactobacillus bulgaricus, 14~18 parts of lactobacillus acidophilus and Lactobacillus plantarum, the probiotic powder The viable bacteria content of agent is 2 × 108~8 × 108cfu/g。
6. the fermentation composition as any one of Claims 1 to 5, it is characterised in that the fermentation composition also includes Parts by weight are 1~3 part of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria；
The preparation method of the fermentation composition also includes：
(1) fermentation composition of the powdery of 1/2 amount is made softwood, is well mixed with the lactic acid bacteria of the weight portion laggard Row extrusion makes ball, is made capsule core；
(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the weight portion, is made It is standby into softwood thing；
(3) obtained capsule core is placed in the coating solution of advance preparation, is coated；
(4) to the round as a ball extruding in the softwood thing of step (2) of the coating capsule core after Cotton seeds, then it is dried, obtains final product pellet Fermentation composition；
Wherein, the coating solution is by the adjacent benzene two of the acroleic acid resin No. II and 0.1-2 parts that parts by weight are 0.4-10 parts Formic acid diethylester adds the aqueous dispersion that appropriate purified water is configured to.
7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step Suddenly：
1) preparation of Chinese medical extract：
The root of kudzu vine of the weight portion, sealwort and cassia seed are crushed respectively, 60 mesh sieves are crossed, be obtained root of kudzu vine powder, Solomonseal Rhizome and Cassia Seed；
By the stem of noble dendrobium, matrimony vine, balsam pear, Chinese yam, Radix Glycyrrhizae, mulberry of root of kudzu vine powder, Solomonseal Rhizome, Cassia Seed and predetermined weight part Leaf, lily keep 4h with 10 times of water of weight at 80-90 DEG C of temperature, are then cooled to 40 DEG C, plus 2 times of weight concentrations are 75% alcohol reflux 4h, filtering, obtains the first filtrate；Addition 3 times of water of weight of filter residue, 2.5h is kept at 80-90 DEG C of temperature, Then be cooled to 30 DEG C, filtering, the second filtrate；Merging filtrate, is concentrated under reduced pressure, freeze-drying, and low-temperature grinding is into being ground into The traditional Chinese medicine fine powder of 0.05-0.2mm；
2) by step 1) in traditional Chinese medicine fine powder mix with emulsifying agent, water after carry out high speed shear with cutter, form grain diameter Chinese medicine particulate less than 50 microns；
3) by the functional oligose of predetermined weight number, resistant dextrin and step 2) in Chinese medicine particulate uniformly mix, pass through Heat exchanger carries out sterilization, and condition is 99 DEG C, 300s；
4) mixture is cooled to 40-45 DEG C after sterilization, adds the probiotics of scheduled volume freeze-dried, adds dry method to make after being well mixed Grain machine granulation, is obtained the fermentation composition of powdery, and aseptic packing is obtained final product.
8. the preparation of the plant enzyme of being nursed one's health suitable for diabetes any one of a kind of claim 1-5 and routine servicing Method, it is characterised in that the described method comprises the following steps：
A () prepares powdery fermentation composition
(b) mixed material：The plant material of 1kg is cleaned, is put into ferment bucket with the sugar of 0.3~0.6kg weight, add 5~6L Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope Lid, lucifuge fermentation is carried out in the environment that fermentation temperature is 28~30 DEG C；
C () is fermented：Stirring sooner or later once daily is carried out to the fermentate in ferment bucket, after 5~8d of fermentation, filtering obtains final product plant Thing ferment.
9. the preparation of the plant enzyme of being nursed one's health suitable for diabetes any one of a kind of claim 1-5 and routine servicing Method, it is characterised in that the described method comprises the following steps：
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added The pure water of 10L, is well mixed, and after standing 1~2d of extraction, carries out high-temperature sterilization, filters, and obtains plant extraction liquid；
C) preparation of plant enzyme stoste：In inoculation composite flora to plant extraction liquid, mixing is placed in closed environment, carries out lucifuge Fermentation 1~2 month, obtains plant enzyme stoste；
Wherein inoculum concentration is 8~12%；Composite flora is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, gram It is several in lactobacillus, lactobacillus acidophilus and acetobacter；
D) preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with And plant material 0.5kg, add the plant enzyme stoste of 150ml to be fermented in closed environment, fermentation temperature is 28~30 DEG C, deflate 1~2 time daily, after 1~2d of fermentation；The fermentation composition of the step of addition 30g a) preparations, fermentation temperature is constant, after Supervention ferment 3-4d, filtering, obtains final product plant enzyme.
10. described in a kind of claim 6 suitable for diabetes conditioning and the preparation method of the plant enzyme of routine servicing, it is special Levy and be, the described method comprises the following steps：
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid：The plant material of 3kg is cleaned, in sugar to the plant material of addition 1kg weight, and is added The pure water of 10L, is well mixed, and after standing 1~2d of extraction, carries out high-temperature sterilization, filters, and obtains plant extraction liquid；
III) the preparation of plant enzyme：From the drum of more than 10L, addition native malt sugar 125g, vitamine C sodium 5g, 8L water And plant extraction liquid 1kg, add 30g the step of II) fermentation composition of pellet for preparing, sent out in closed environment Ferment；Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily；After 1~2d of fermentation, insertion conduit is passed through air, and ventilate 2-4 daily It is secondary, each 10-30min, and it is 35~37 DEG C to control fermentation temperature；Filtered after 5~7d of fermentation, obtain final product plant enzyme.
CN201710234608.4A 2017-04-11 2017-04-11 Prepare the fermentation composition and preparation method of the plant enzyme for being applied to diabetes conditioning and routine servicing Pending CN106858579A (en)

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Publication number Priority date Publication date Assignee Title
CN107125527A (en) * 2017-07-13 2017-09-05 厦门元之道生物科技有限公司 A kind of sugar-free fermented beverage for being available for diabetes patient to use and preparation method thereof
CN108056469A (en) * 2017-11-28 2018-05-22 齐鲁工业大学 The preparation method of seaweed ferment with function of blood sugar reduction
CN110604789A (en) * 2018-06-15 2019-12-24 南京大学 Application of dendrobium officinale and extract thereof in combination with probiotics in improvement of diabetes and metabolic syndrome
CN110693006A (en) * 2019-10-18 2020-01-17 广州工商学院 Carambola enzyme fermentation method and fermentation device
CN111802632A (en) * 2020-06-05 2020-10-23 福贡壹壹农业生物有限公司 Preparation method of polygonatum kingianum enzyme
CN112075626A (en) * 2020-09-23 2020-12-15 山东向日葵生物工程有限公司 Traditional Chinese medicine fermented beverage for tonifying qi and enriching blood and using method and preparation method thereof
TWI730719B (en) * 2020-04-13 2021-06-11 陳俊宏 Plant fermented product, method of manufacturing the same, composition of plant fermented product and use thereof

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CN103859378A (en) * 2012-12-10 2014-06-18 天津红日药业股份有限公司 Formula and preparation method of life cultivation and health preservation food helpful for reducing blood sugar
CN105249326A (en) * 2015-10-15 2016-01-20 维尔信科技（潍坊）有限公司 Fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and preparation method of fermented fruit and vegetable composition
CN106072604A (en) * 2016-06-07 2016-11-09 佛山市朗达信息科技有限公司 There is weight reducing and remove compositions and the preparation thereof of fat effect
CN106072578A (en) * 2016-07-11 2016-11-09 佛山市朗达信息科技有限公司 There is the enhancing enzyme composition of immune function of human body, effervescent tablet and preparation method

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CN103859378A (en) * 2012-12-10 2014-06-18 天津红日药业股份有限公司 Formula and preparation method of life cultivation and health preservation food helpful for reducing blood sugar
CN105249326A (en) * 2015-10-15 2016-01-20 维尔信科技（潍坊）有限公司 Fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and preparation method of fermented fruit and vegetable composition
CN106072604A (en) * 2016-06-07 2016-11-09 佛山市朗达信息科技有限公司 There is weight reducing and remove compositions and the preparation thereof of fat effect
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107125527A (en) * 2017-07-13 2017-09-05 厦门元之道生物科技有限公司 A kind of sugar-free fermented beverage for being available for diabetes patient to use and preparation method thereof
CN108056469A (en) * 2017-11-28 2018-05-22 齐鲁工业大学 The preparation method of seaweed ferment with function of blood sugar reduction
CN110604789A (en) * 2018-06-15 2019-12-24 南京大学 Application of dendrobium officinale and extract thereof in combination with probiotics in improvement of diabetes and metabolic syndrome
CN110693006A (en) * 2019-10-18 2020-01-17 广州工商学院 Carambola enzyme fermentation method and fermentation device
TWI730719B (en) * 2020-04-13 2021-06-11 陳俊宏 Plant fermented product, method of manufacturing the same, composition of plant fermented product and use thereof
CN111802632A (en) * 2020-06-05 2020-10-23 福贡壹壹农业生物有限公司 Preparation method of polygonatum kingianum enzyme
CN112075626A (en) * 2020-09-23 2020-12-15 山东向日葵生物工程有限公司 Traditional Chinese medicine fermented beverage for tonifying qi and enriching blood and using method and preparation method thereof

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