CN101926428B - Probiotic gingko pollen freeze-dried powder and preparation method thereof - Google Patents
Probiotic gingko pollen freeze-dried powder and preparation method thereof Download PDFInfo
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- CN101926428B CN101926428B CN2010102416723A CN201010241672A CN101926428B CN 101926428 B CN101926428 B CN 101926428B CN 2010102416723 A CN2010102416723 A CN 2010102416723A CN 201010241672 A CN201010241672 A CN 201010241672A CN 101926428 B CN101926428 B CN 101926428B
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Abstract
The invention provides probiotic gingko pollen freeze-dried powder and a preparation method thereof. The probiotic gingko pollen freeze-dried powder is live bacteria freeze-dried powder prepared by utilizing wall-broken or non-wall-broken gingko pollen as a raw material, additionally adding brown sugar at a ratio, fermenting by individual plant of Lactobacillus rhamnosus or Lactobacillus acidophilus or the combination of the two probiotics, and freeze drying. The probiotic gingko pollen freeze-dried powder has probiotic viable count of above 109cfu/g, contains a great amount of gingko pollen functional components and probiotic fermentation products, and has the effects of enhancing immunity, regulating intestinal flora, preventing constipation and the like. The freeze-dried powder has stable quality, small dosage, and easy carry-over, administration and storage, is prepared by fermenting pure natural gingko pollen through probiotics, is added with no chemical synthesis substances, has high safety and unique functions, and can be taken in a long time.
Description
Technical field:
The present invention relates to food technology field, relate in particular to a kind of ginkgo composition that had both contained functions such as anticancer, enhance memory and raising immunity, have the beneficial flora that to regulate the stomach and intestine balance again, integrate health care and the probiotic gingko pollen freeze-dried powder of treating; The invention still further relates to the preparation method of this probiotic gingko pollen freeze-dried powder,
Background technology:
The raising of Along with people's health perception, health food more and more cause people's attention.Adopt the Chinese herbal medicine of integration of drinking and medicinal herbs and the probio development product of improving the stomach and intestine balance, become the development trend of this industry.To be that China is distinctive treasure plant to ginkgo, is called as botanic " living fossil ".Ginkgo is precious from head to foot, and its fruit, leaf, seedling have important edible, medicinal and ornamental value.At present, utilize the extract (like GBE761) of ginkgo leaf and ginkgo nut to develop a variety of medicines and health products both at home and abroad, and very fast-selling.But fewer for the research of gingko pollen, domestic literature has only been reported morphological feature, grows chemical composition, the achievement in research of aspects such as biologically active and artificial pollination.At present, also, do not see the sale that gingko pollen health food and medicine are arranged on the market not about the report of gingko pollen product development yet.Gingko pollen is rich in the necessary amino acid of human body, flavones, and lactone, soluble sugar, organic acid, unrighted acid, nucleic acid, enzyme, indispensable mineral element of human body and vitamin E have high nourishing healthy and medical value to human body.Therefore, research and development gingko pollen technology and product, significant.
Probio is meant improves host's microecological balance and brings into play beneficial effect; Reach the active bacteria formulation and the metabolite thereof that improve host health level and health status; Probio is present in tellurian each corner, and bacteria beneficial or fungi mainly contain in the animal body: lactic acid bacteria, Bifidobacterium, saccharomycete etc.Lactic acid bacteria is important probio, and biodiasmin can be secreted the material useful to health when growth, therefore human body is had more benefit.1, the effect that promotes gastric secretion, raising appetite, strengthens digestion is arranged; Probio can be decomposed lactose in milk and protein, makes human body more be prone to digestion and absorption; 2, safeguard the gut flora ecological balance, form biological barrier, suppress of the invasion of harmful bacterium enteron aisle; 3, can reduce the generation of some carcinogen, thereby protective effect on cancer risk is arranged; 4, can suppress the breeding of spoilage organisms in the enteron aisle, and weaken the toxin that spoilage organisms produces in enteron aisle; Promote intestines peristalsis and thalline raised growth change osmotic pressure to prevent constipation through producing a large amount of SCFAs; 5, the effect that reduces cholesterol is arranged, the people of suitable especially high fat of blood drinks; 6, improve immune function of human body, lactic acid bacteria can produce the material of some raise immunity, can improve human immunity, wards off disease.In general, no matter be after performing the operation, or the patient after acute, chronic the recovering; In order to treat disease or to protect from infection and all once took or injected a large amount of antibiotic; Make the intestinal burst give birth to very about-face, some useful intestinal are suppressed or kill, and cause flora imbalance.Take a certain amount of beneficial natural disposition lactic acid bacteria every day, can keep the gut flora balance, regulate the intestinal beneficial bacterium crowd to normal level.
Probiotic gingko pollen freeze-dried powder the is compound health care of two kinds of single products; The ginkgo composition that had both contained functions such as anticancer, enhance memory and raising immunity; Has the beneficial flora that to regulate the stomach and intestine balance again; Integrate health care and treat, the medical science viewpoint that preceding people's prevention, health care, treatment combine with rehabilitation, market prospects will be more extensive.
Summary of the invention:
One of the object of the invention provides a kind of ginkgo composition that had both contained functions such as anticancer, enhance memory and raising immunity, has the beneficial flora that can regulate the stomach and intestine balance again, integrates health care and the probiotic gingko pollen freeze-dried powder of treating; Two of purpose provides the preparation method of this probiotic gingko pollen freeze-dried powder.
One of the object of the invention can be realized through following technical measures:
The probiotic gingko pollen freeze-dried powder of basic invention is to be raw material with the gingko pollen then of 10～40 weight portions and the brown sugar of 5～20 weight portions; The water that adds 40～70 weight portions; Through Lactobacillus rhamnosus, two kinds of probio individual plants of lactobacillus acidophilus or mixed solid fermentation, and then the viable bacteria freeze-dried powder that forms through freeze drying.
One of the object of the invention also can be realized through following technical measures:
Described gingko pollen is meant broken wall or broken wall gingko pollen not.
Two of the object of the invention can be realized through following technical measures:
(1). get gingko pollen and use the micronizer broken wall treatment, cross the 500-800 mesh sieve, get the broken wall gingko pollen;
(2). the probio activation:
A, culture medium: adopt the MRS culture medium, 121 ℃ of sterilization 30min earlier after the packing preserve in refrigerator after putting into the inclined-plane again, obtain the MRS slant medium;
B, slant strains: with bacterial classification freeze-drying pipe sterilize, sever, the line of MRS solid medium cultivates, and separates colonies typical and passes the inclined-plane seed culture medium, cultivates 10-14hr for 30-37 ℃, preserve in refrigerator then, female inclined-plane seed;
C, slant activation: be inoculated in sub-inclined-plane by female inclined-plane seed, cultivate 10-14hr for sub-inclined-plane 30-37 ℃;
(3). liquid spawn: be inoculated in liquid MRS culture medium by sub-inclined-plane, cultivate 10～14hr for 30-37 ℃,
(4). bacterial classification enlarges liquid: the preparation weight content is the brown sugar water of 3-5%, and heated and boiled 30-40min is cooled to normal temperature, and with the ratio inoculation liquid spawn of 1-3%, 30-37 ℃ leaves standstill and cultivate 3～4dr, treats that liquid reduces to pH=3.0-3.5, bacterial classification enlarges liquid;
(5). fermentation: according to the broken wall gingko pollen: brown sugar: water=10～40: 5～20: 40～70 weight portion proportionings are mixed; 5～10% weight portion bacterial classifications that the sterilization back adds mixed liquor enlarge liquid; Mixing bacterium cultivates with two bacterial classifications of Lactobacillus rhamnosus and two kinds of probios of lactobacillus acidophilus and enlarges liquid in 1: 1 ratio adding; The 30-37 ℃ of static cultivation 24～72hr of sealing treats that zymotic fluid reduces to pH=3.0-3.5, tunning;
(6). freeze drying: with above-mentioned tunning in-20～-80 ℃ of following freeze drying to wet weights less than 3%, number of live bacteria of probiotics is greater than 10
9Cfu/g, product is preserved in the low temperature lower seal.
The present invention be directed to the general sub-health state of modern society people; Provide a kind of with broken wall then or not the broken wall gingko pollen be raw material; Be equipped with a certain proportion of brown sugar, get the viable bacteria freeze-dried powder through Lactobacillus rhamnosus, two kinds of probio individual plants of lactobacillus acidophilus or mixed culture fermentation and freeze drying.Number of live bacteria of probiotics reaches 10
9More than the cfu/g, and contain a large amount of gingko pollen functional components and probiotics fermention product, like dietary fiber, oligosaccharides, multiple essential amino acid, flavones, organic acid etc., have enhance immunity power, regulate functions such as gut flora and prevention constipation.This freeze-dried powder steady quality, dose is little, is easy to carry about with one to take and store, and is formed through probiotics fermention by the gingko pollen of pure natural, does not add any chemical synthesis material, and is safe, and function is unique, can take for a long time.
Probiotic gingko pollen freeze-dried powder of the present invention; Contain health-care components such as a large amount of probiotic lactobacillus and essential amino acid, dietary fiber, polysaccharide, oligosaccharides, flavones, lactone; The health care of gingko pollen and probio is mutually promoted, integrate health care and treat.
Preparation method of the present invention is simple, handled easily, non-environmental-pollution.
The specific embodiment:
Below in conjunction with embodiment the present invention is further specified, but be not limited thereto.
Embodiment 1:
Preparation method of the present invention is following:
(1). get then not the broken wall gingko pollen and use the micronizer broken wall treatment, cross 500 mesh sieves, the broken wall gingko pollen;
(2). the probio activation:
A, culture medium: adopt the MRS culture medium, 115 ℃ of sterilization 30min earlier after the packing preserve in refrigerator after putting into the inclined-plane again, obtain the MRS slant medium;
B, slant strains: with bacterial classification freeze-drying pipe sterilize, sever, the line of MRS solid medium cultivates, and separates colonies typical and passes the inclined-plane seed culture medium, cultivates 14hr for 30 ℃, preserve in refrigerator then, female inclined-plane seed;
C, slant activation: be inoculated in sub-inclined-plane by female inclined-plane seed, 14hr is cultivated for 30 ℃ in sub-inclined-plane;
(3). liquid spawn: be inoculated in liquid MRS culture medium by sub-inclined-plane, cultivate 14hr for 30 ℃, get liquid spawn;
(4). bacterial classification enlarges liquid: the preparation weight content is 3% brown sugar water, and heated and boiled 40min is cooled to normal temperature, the ratio inoculation liquid spawn with 1%, 37 ℃ leave standstill and cultivate 3dr, treat that liquid reduces to pH=3.5, bacterial classification enlarges liquid;
(5). fermentation: according to the broken wall gingko pollen: brown sugar: water=the weight portion proportioning was mixed in 10: 20: 40; The 10% weight portion bacterial classification that the sterilization back adds mixed liquor enlarges liquid; Mixing bacterium cultivates with two bacterial classifications of Lactobacillus rhamnosus and two kinds of probios of lactobacillus acidophilus and enlarges liquid in 1: 1 ratio adding; 30 ℃ of static cultivations 72hr of sealing treat that zymotic fluid reduces to pH=3.0, must tunning;
(6). freeze drying: with above-mentioned tunning in-80 ℃ of pre-freeze 1hr, and then freeze drying 72hr, control product wet weight is less than 3%, number of live bacteria of probiotics is greater than 10
9Cfu/g, product is preserved in the low temperature lower seal.
Embodiment 2:
Preparation method of the present invention is following:
(1). get then not the broken wall gingko pollen and use the micronizer broken wall treatment, cross 800 mesh sieves, the broken wall gingko pollen;
(2). the probio activation:
A, culture medium: adopt the MRS culture medium, 120 ℃ of sterilization 15min earlier after the packing preserve in refrigerator after putting into the inclined-plane again, obtain the MRS slant medium;
B, slant strains: with bacterial classification freeze-drying pipe sterilize, sever, the line of MRS solid medium cultivates, and separates colonies typical and passes the inclined-plane seed culture medium, cultivates 10hr for 37 ℃, preserve in refrigerator then, female inclined-plane seed;
C, slant activation: be inoculated in sub-inclined-plane by female inclined-plane seed, 10hr is cultivated for 37 ℃ in sub-inclined-plane;
(3). liquid spawn: be inoculated in liquid MRS culture medium by sub-inclined-plane, cultivate 10hr for 37 ℃, get liquid spawn;
(4). bacterial classification enlarges liquid: the preparation weight content is 5% brown sugar water, and heated and boiled 30min is cooled to normal temperature, the ratio inoculation liquid spawn with 3%, 30 ℃ leave standstill and cultivate 4dr, treat that liquid reduces to pH=3.0, bacterial classification enlarges liquid;
(5). fermentation: according to the broken wall gingko pollen: brown sugar: water=the weight portion proportioning was mixed in 40: 5: 70; The 5% weight portion bacterial classification that the sterilization back adds mixed liquor enlarges liquid; Mixing bacterium cultivates with two bacterial classifications of Lactobacillus rhamnosus and two kinds of probios of lactobacillus acidophilus and enlarges liquid in 1: 1 ratio adding; 37 ℃ of static cultivations 24hr of sealing treat that zymotic fluid reduces to pH=3.5, must tunning;
(6). freeze drying: with above-mentioned tunning in-20 ℃ of pre-freeze hr, and then freeze drying 24hr, control product wet weight is less than 3%, number of live bacteria of probiotics is greater than 10
9Cfu/g, product is preserved in the low temperature lower seal.
Embodiment 3:
Preparation method of the present invention is following:
(1). get then not the broken wall gingko pollen and use the micronizer broken wall treatment, cross 600 mesh sieves, the broken wall gingko pollen;
(2). the probio activation:
A, culture medium: adopt the MRS culture medium, 118 ℃ of sterilization 20min earlier after the packing preserve in refrigerator after putting into the inclined-plane again, obtain the MRS slant medium;
B, slant strains: with bacterial classification freeze-drying pipe sterilize, sever, the line of MRS solid medium cultivates, and separates colonies typical and passes the inclined-plane seed culture medium, cultivates 12hr for 33 ℃, preserve in refrigerator then, female inclined-plane seed;
C, slant activation: be inoculated in sub-inclined-plane by female inclined-plane seed, 12hr is cultivated for 35 ℃ in sub-inclined-plane;
(3). liquid spawn: be inoculated in liquid MRS culture medium by sub-inclined-plane, cultivate 12hr for 33 ℃, get liquid spawn;
(4). bacterial classification enlarges liquid: the preparation weight content is 4% brown sugar water, and heated and boiled 35min is cooled to normal temperature, the ratio inoculation liquid spawn with 2%, 33 ℃ leave standstill and cultivate 3.5dr, treat that liquid reduces to pH=3.3, bacterial classification enlarges liquid;
(5). fermentation: according to the broken wall gingko pollen: brown sugar: water=the weight portion proportioning was mixed in 25: 12: 60; The 8% weight portion bacterial classification that the sterilization back adds mixed liquor enlarges liquid; Mixing bacterium cultivates with two bacterial classifications of Lactobacillus rhamnosus and two kinds of probios of lactobacillus acidophilus and enlarges liquid in 1: 1 ratio adding; 33 ℃ of static cultivations 48hr of sealing treat that zymotic fluid reduces to pH=3.2, must tunning;
(6). freeze drying: with above-mentioned tunning in-50 ℃ of pre-freeze 1.5hr, and then freeze drying 48hr, control product wet weight is less than 3%, number of live bacteria of probiotics is greater than 10
9Cfu/g, product is preserved in the low temperature lower seal.
Claims (2)
1. the preparation method of a probiotic gingko pollen freeze-dried powder; It is characterized in that it is is raw material with the gingko pollen then of 10～40 weight portions and the brown sugar of 5～20 weight portions; The water that adds 40～70 weight portions; Through Lactobacillus rhamnosus, two kinds of probio mixed solid fermentations of lactobacillus acidophilus, and then the viable bacteria freeze-dried powder that forms through freeze drying; Described gingko pollen is meant broken wall or broken wall gingko pollen not, and described broken wall gingko pollen is to get not broken wall gingko pollen, uses the micronizer broken wall treatment, crosses the 500-800 mesh sieve;
The preparation process is:
(1) probio activation:
A, culture medium: adopt the MRS culture medium, first 115-120 ℃ of sterilization 15-30min after the packing preserves in refrigerator after putting into the inclined-plane again, obtains the MRS slant medium;
B, slant strains: with bacterial classification freeze-drying pipe sterilize, sever, the line of MRS solid medium cultivates, and separates colonies typical and changes the inclined-plane seed culture medium, cultivates 10-14hr for 30-37 ℃, preserve in refrigerator then, female inclined-plane seed;
C, slant activation: be inoculated in sub-inclined-plane by female inclined-plane seed, cultivate 10-14hr for sub-inclined-plane 30-37 ℃;
(2) liquid spawn: be inoculated in liquid MRS culture medium by sub-inclined-plane, cultivate 10-14hr for 30-37 ℃, get liquid spawn;
(3) bacterial classification enlarges liquid: the brown sugar water of preparation 3-5% weight, heated and boiled 30-40min is cooled to normal temperature, with the ratio inoculation liquid spawn of 1-3%, 30-37 ℃ leaves standstill and cultivated 3～4 days, treats that liquid reduces to pH=3.0-3.5, bacterial classification enlarges liquid;
(4) fermentation: according to broken wall or broken wall gingko pollen not: brown sugar: water=10～40: 5～20: 40～70 weight portion proportionings are mixed; 5～10% weight bacterial classifications that the sterilization back adds mixed liquor enlarge liquid; It is to enlarge liquid in 1: 1 ratio adding with two bacterial classifications of Lactobacillus rhamnosus and two kinds of probios of lactobacillus acidophilus that said bacterial classification enlarges liquid; The 30-37 ℃ of static cultivation 24～72hr of sealing treats that zymotic fluid reduces to pH=3.0-3.5, tunning;
(5) freeze drying: with above-mentioned tunning in-20～-80 ℃ of following freeze drying to water content less than 3%, product is preserved in the low temperature lower seal.
2. the probiotic gingko pollen freeze-dried powder of processing with the preparation method of the described a kind of probiotic gingko pollen freeze-dried powder of claim 1.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102318847B (en) * | 2011-08-23 | 2012-10-31 | 范文杰 | Fermented black ginkgo kernels, and preparation method thereof |
| CN103053893B (en) * | 2012-12-25 | 2014-01-22 | 许正鼎 | Producing technique of natural pollen fermenting products |
| CN103614429B (en) * | 2013-11-19 | 2015-06-24 | 山东省农业科学院农产品研究所 | Method for transforming gingko pollen flavonoid glycoside employing microorganisms |
| CN105309856B (en) * | 2014-12-04 | 2019-09-03 | 山东省农业科学院农产品研究所 | A kind of gingko pollen fermentation oral liquid and preparation method thereof |
| CN108113001B (en) * | 2017-12-28 | 2021-03-30 | 福建拓天生物科技有限公司 | Probiotic freeze-dried powder for relieving gastric ulcer |
| CN108378356A (en) * | 2018-02-27 | 2018-08-10 | 北京秦凰达同生物科技有限公司 | A kind of broken wall crushes the preparation method of fermented food |
| CN112940985A (en) * | 2021-03-31 | 2021-06-11 | 盐城维康生物科技有限公司 | Lactobacillus rhamnosus preparation for enhancing human immunity and preparation method thereof |
| CN114601015A (en) * | 2022-03-16 | 2022-06-10 | 华中农业大学 | Preparation method of probiotic soybean protein plant cheese |
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| CN1853505A (en) * | 2004-06-13 | 2006-11-01 | 刘开林 | Edible ginkgo pollen |
| CN101422196A (en) * | 2008-10-31 | 2009-05-06 | 上海双金生物科技有限公司 | Functional yoghourt and production method thereof |
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| KR20050024872A (en) * | 2003-09-05 | 2005-03-11 | 주식회사 에스앤텍 | Fermented pollen extracts with antimicrobial activity |
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| CN1853505A (en) * | 2004-06-13 | 2006-11-01 | 刘开林 | Edible ginkgo pollen |
| CN101422196A (en) * | 2008-10-31 | 2009-05-06 | 上海双金生物科技有限公司 | Functional yoghourt and production method thereof |
| CN101642478A (en) * | 2009-09-09 | 2010-02-10 | 王学林 | Production method of organic vitamin pine pollen |
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