CN107568537A - A kind of compound microorganism ferments beverage containing anthocyanidin and preparation method thereof - Google Patents
A kind of compound microorganism ferments beverage containing anthocyanidin and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to microbial fermentation technical field of beverage, it is less in order to solve the research report about the pure microbial fermentation beverage of anthocyanidin in existing food and drink, lack the pure microbial fermentation beverage containing anthocyanidin has the problem of product of function type health care beverage of integration of drinking and medicinal herbs again simultaneously, there is provided a kind of compound microorganism ferments beverage containing anthocyanidin and preparation method thereof.Mixed by a certain percentage by purple sweetpotato, grape, blueberry, strawberry, mulberry fruit, hawthorn and water, the microbe leaven prepared with Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus acidophilus, bifidobacterium breve and saccharomyces cerevisiae is fermented, and adds collagen polypeptide and stachyose is prepared.Protection liver, pre- anti-cancer, eyesight, the propagation removing free radical harmful in vivo, improve sleep, promoting internal beneficial bacterium can be promoted with anti-oxidant, anti-mutation, antiallergy, prevention cardiovascular and cerebrovascular disease, suppresses the breeding of harmful bacteria；There is good adjuvant treatment effect to various chronic diseases.
Description
Technical field
The invention belongs to microbial fermentation technical field of beverage, and in particular to a kind of compound microorganism ferments are drunk containing anthocyanidin
Material and preparation method thereof.
Background technology
At present, food and drink can be divided into fruit juice and its beverage, vegetable juice and its beverage, phytoprotein beverage, plant and take out
Extract beverage, milk beverage, soda, mineral water, solid beverage and Other Drinks.Beverage types are various, and market is huge, and have
The pass pure microbial fermentation beverage of anthocyanidin is that the research of integration of drinking and medicinal herbs function type health care beverage is less again simultaneously.
Anthocyanidin is a kind of water-soluble flavonoids pigment being widely present in plant, and its concentration is with season and cell liquid
The change of composition and change so that multicolour is presented in petal and fruit.The research of anthocyanidin starts from the eighties in last century, main
Concentrate on the research of the extracting and developing, purifying process of anthocyanidin；Nearly ten years, more and more in depth ground with to anthocyanidin
Study carefully, its physiologically active and function and its mechanism of action progressively turn into the focus of numerous scholar's research.Again because the health of people
Consciousness increasingly strengthens, and the edible of anthocyanidin, medical value get the attention and approved, including its anti-oxidant, antiinflammatory action,
Bacteriostasis, anti-aging, antitumaous effect and to the physiologically active such as liver, the protective effect to cardiovascular and cerebrovascular and eyesight.
The content of the invention
The present invention in order to the research solved in existing food and drink about the pure microbial fermentation beverage of anthocyanidin report it is less,
Lack the pure microbial fermentation beverage containing anthocyanidin simultaneously and there is asking for the product of the function type health care beverage of integration of drinking and medicinal herbs
A kind of topic, there is provided compound microorganism ferments beverage containing anthocyanidin and preparation method thereof.The beverage containing anthocyanidin obtained can be with
Anti-oxidant, anti-mutation, antiallergy, prevention cardiovascular and cerebrovascular disease, protection liver, pre- anti-cancer, enhancement eyesight, removing are harmful in vivo
Free radical, improve sleep, promote the propagation of internal beneficial bacterium, suppress the breeding of harmful bacteria；Have to various chronic diseases good
Adjuvant treatment effect.
What the present invention was realized by following technical scheme：A kind of compound microorganism ferments beverage containing anthocyanidin, including with the following group
Divide and each component ratio is：Purple sweetpotato 5-10%, grape 5-10%, blueberry 5-10%, strawberry 5-10%, mulberry fruit 5-10%, hawthorn 5-
10%th, collagen polypeptide 1%, microbe leaven 0.1%, stachyose 0.5%, remaining is water；The microbe leaven is：Plant breast
Bacillus（Lactobacillus plantarum）CICC20265 zymotic fluids, Lactobacillus rhamnosus（Lactobacillus
rhamnosus）CICC6136 zymotic fluids, lactobacillus acidophilus（Lactobacillus acidophilus）CICC6083 ferments
Liquid, bifidobacterium breve（Bifidobacterium breve）CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces
cerevisiae）CICC1624 zymotic fluids are 1 according to weight ratio:1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml。
A kind of method of compound microorganism ferments beverage containing anthocyanidin is prepared, is comprised the following steps that：
（1）Fruit pre-processes：Purple sweetpotato, grape, blueberry, strawberry, mulberry fruit, hawthorn are cleaned respectively, crushed after mixing in proportion,
Then plus fruit juice liquid medium is made in water, standby after sterilizing；
（2）The preparation of microbe leaven：The preparation of zymotic fluid：Zymotic fluid culture medium prescription is：Peptone 10.1g, beef extract
10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, distilled water 1.0L, by Lactobacillus plantarum（Lactobacillus
plantarum）CICC20265, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136, lactobacillus acidophilus
（Lactobacillus acidophilus）CICC6083, bifidobacterium breve（Bifidobacterium breve）
CICC6185, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 is aseptically inoculated with into fermentation respectively
In liquid culture medium, 28 DEG C~32 DEG C single Spawn incubations 24~48 hours, obtain respectively Lactobacillus plantarum bacterium number >=5.0 ×
109Cfu/ml zymotic fluid, Lactobacillus rhamnosus bacterium number >=5.0 × 109Cfu/ml zymotic fluid, lactobacillus acidophilus bacterium number >=5.0
×109Cfu/ml zymotic fluid, bifidobacterium breve bacterium number >=5.0 × 109Cfu/ml zymotic fluid, saccharomyces cerevisiae bacterium number >=5.0 ×
109Cfu/ml zymotic fluid；By single strain fermentating liquid of acquisition according to weight ratio be 1:1:1:1：1 ratio mixing is micro- life
Thing leavening；
（3）Drink fermented：In the fermentation tank to have sterilized, by step（1）0.1% is added in the fruit juice liquid medium to have sterilized
Microbe leaven, the volume ratio of the zymotic fluid in the volume and fermentation tank that oxygen dissolved is oxygen in 5% i.e. fermentation tank is 5%
Under the conditions of, 35 DEG C~37 DEG C closed quiescent cultures 72 hours, pH value drops to 3.0~3.5 and produces complex microorganism hair in fermentation tank
Ferment beverage containing anthocyanidin.
Anthocyanidin content is 100mg/500g in the compound microorganism ferments beverage containing anthocyanidin.
Strain function used in the present invention is as follows：
Lactobacillus plantarum and the difference of other lactic acid bacterias are that the viable count of this bacterium is higher, can largely produce acid, make in water
PH value is stable not to be raised, and the acidic materials of its output can degrade heavy metal；Because this bacterium is anaerobic bacteria（It is facultative aerobic）,
The energy distinctive lactobacillin of output in reproductive process, lactobacillin is a kind of preservative of bion.Lactobacillus plantarum
With many health-care effects, such as：There is certain immunoregulation effect；There is inhibitory action to pathogenic bacteria；Reduce serum cholesterol
Content and prevention of cardiovascular disease；Maintain intestinal flora balance；Promote absorption of nutrient ingredients；Alleviate lactose intolerance；Suppress
Formation of tumour cell etc..
Lactobacillus rhamnosus (L.rhamnosus), is subordinated to lactobacillus, is one of Body normal flora, and enteron aisle sticks together
Rate is high, and colonization ability is strong, and has efficient norcholesterol, promotes cell division, can play regulation gut flora, prevention and treatment
Diarrhoea, exclude toxin, pre- anti-caries, improve the important physiological hygiene function such as immunity of organisms and anticancer.Lactobacillus rhamnosus
Be resistant to animal alimentary canal environment, be colonized in humans and animals enteron aisle, play regulation gut flora, prevention and treatment diarrhoea,
Exclude toxin and improve immunity of organisms and other effects.
Lactobacillus rhamnosus is resistant to animal alimentary canal environment, is colonized in humans and animals enteron aisle, plays regulation enteron aisle
Flora, prevention and treatment diarrhoea, exclude toxin and improve immunity of organisms and other effects.The Lactobacillus rhamnosus of high-purity is for mistake
Quick constitution has good mitigation.Lactobacillus rhamnosus, which has, balances and improves gastrointestinal function；Strengthen human autoimmune
Ability；Promote Bifidobacterium and lactobacillus acidophilus growth and effect；Diarrhoea is treated in prevention and help；Preventing respiratory tract infection；Row
Go out toxin；Pre- anti-caries；It is pre- hypo-allergenic.
Lactobacillus acidophilus belongs to lactobacillus, and Gram-positive bacillus, the end of bar is rounded, is primarily present in small intestine,
Lactic acid, acetic acid and some antibiotic to be worked to harmful bacteria are discharged, but beneficial bacteria effect is weaker.Lactobacillus acidophilus is under one's belt
Survival rate can typically reach 80%, if but without embedding treatment, after oral lactobacillus acidophilus, their vigor is still can be by
Influence；And if all of which embedded, its beneficial effect under one's belt can not be played again.Lactobacillus acidophilus is whole in human body
In intestines and stomach, " from top to down " regulation micro-ecological environment.
For Bifidobacterium in human body intestinal canal to health important role, Bifidobacterium is the health mark of human body,
It is the profitable strain for having many benefits to human body.There is Bifidobacterium, child's period Bifidobacterium number in 3-4 months in baby due
Amount gradually increases and reaches peak, accounts for the 25% of intestinal bacterium total amount, with the increase at age, bifidobacteria is passed year by year
Subtract, senectitude bifidobacteria is decremented to less than 7.9%.
The quantity of profitable strain-Bifidobacterium, the propagation of Bifidobacterium can change in stachyose energy selective proliferative enteron aisle
Kind intestinal environment, the growth for suppressing intestinal harmful bacillus, body immunity is improved, there is weight to Constipation, anti-disease, anti-aging
Act on.
Although saccharomycete form is simple, physiology is more complicated, species also compare it is more, using being also many.
It is commercially used for making wine, saccharomycete sucks the monose such as glucose, fructose, mannose into the cell, in the absence of oxygen, passes through
The effect of interior enzyme, monose is decomposed into carbon dioxide and alcohol.This effect is fermented.In medicine, because saccharomycete is rich in dimension life
Plain B, protein and a variety of enzymes, thalline can be made into aluzyme.Indigestion is treated, and production nucleic acid can be extracted from saccharomycete
Derivative, coacetylase, cromoci, the raw material of glutathione and several amino acids.
Drinks yeast is divided into distillery yeast, aroma-producing yeasts, Whiskey yeast.Distillery yeast is divided into Chinese yeast brewer yeast, big again
Bent wine yeast, wheat distillery yeast, sugarcane distillery yeast.
Saccharomyces cerevisiae can protect anthocyanidin not to be destroyed, and Lactobacillus rhamnosus promotes lactobacillus acidophilus and Bifidobacterium
Breeding.
In the raw material that the present invention uses：
Purple sweetpotato（English name：Purple sweet potato）Refer to the sweet potato that potato meat color is purple.Due to rich in anthocyanidin
Spy's kind is being identified as in recent years to the health substance of human nutrition Deng one kind.Purple sweetpotato purple skin, purple meat all edibles, taste
Omit sweet tea in road.20~180mg/100 grams of anthocyanidin content.There is higher edible and medical value, be a kind of pure natural health care food
Product purple sweet potato yield is high, and Flavonoid substances content is higher, and inoxidizability is good, it is often more important that it is a kind of natural harmless
Product, can be used as flight against senium of human body health products, and purple sweet potato has the good prospect of marketing.
Grape is not only delicious and tasty, and nutritive value is very high.Grape sugar content is up to 10%-30% in ripe berry,
Based on glucose.A variety of tartaric acid in grape help to digest, appropriate to eat a little grapes more, can strengthening the spleen and stomach.Contain in grape
Mineral calcium, potassium, phosphorus, iron and multivitamin B1, vitamin B2, vitamin B6, vitamin C and citrin etc., also contain
The amino acid of a variety of needed by human body, often feeding grape are of great advantage to neurasthenia, excessive fatigue.Research finds that grape is than Ah Si
Woods can preferably prevent thrombosis, and can reduce human serum cholesterol levels, reduce hematoblastic cohesiveness, to prevention
Cardiovascular and cerebrovascular diseases have certain effect.Appropriate fresh grape is eaten daily, can not only reduce the onset risk of angiocardiopathy, also spy
It is not beneficial to the health of those ischemic heart diseases and atherosclerotic heart disease patient.Flavonoids thing in fresh grape
Matter, energy " cleaning " blood, prevents the formation of cholesterol plaques.Grape is more in black, more containing Flavonoid substances, if but by grape
Skin and grape pip are eaten together, and the protective effect to heart is more preferably.The traditional Chinese medical science thinks that grape is mild-natured, sweet acid, enters lung, spleen, kidney channel,
Fill blood, liver and kidney benefiting, born fluid, strengthening the bones and muscles, cough-relieving relieving restlessness, tonifying Qi and blood, tonneau urine the effect of.White lamb's-quarters in Grape Skin
Procyanidin content in reed alcohol, grape pip is all higher than other positions of grape, also above other most of fruit trees, and has pole
High medical value, have become global important nutrition and medicinal commodity.
Blueberry averagely weighs 0.5~2.5g, maximum weight 5g, and fruit colour is beautiful, pleasing, blue and by 1 layer of white fruit
Powder, pulp is fine and smooth, and seed is minimum, edible rate 100%, has light fragrance, sweet and sour palatability, good merchantable brand is eaten raw for one.In Blueberry
In addition to the sugar, acid and Vc of routine, rich in VE, VA, VB, SOD, ursin, protein, anthocyanin, dietary fibre and enrich
The mineral element such as K, Fe, Zn, Ca.Anthocyanin pigments content is up to 163mg/100g, fresh fruit VE contents 9.3L in fruit | g/
100g fresh fruits, it is several times of other fruit such as apple, grape even tens times.Total amino acid content 0.254%, contains than amino acid
The abundant hawthorn of amount is also high.Blueberry has extremely strong medical value and alimentary health-care function, and international food and agricultural organization is classified as
One of five big healthy food of the mankind.
Strawberry nutrition value is abundant, is described as being " fruit queen ", contains abundant vitamin C, vitamin A, vitamin
E, nicotinic acid, vitamin B1, vitamin B2, carrotene, tannic acid, aspartic acid, copper, strawberry amine, pectin, cellulose, leaf
The nutriments such as acid, iron, calcium, ellagic acid and anthocyanidin.Especially contained vitamin C, its content are all higher 7- than apple, grape
10 times.And contained malic acid, citric acid, vitamin B1, vitamin B2, and the content of carrotene, calcium, phosphorus, iron also compare apple
Fruit, pears, grape are high 3 to 4 times.Rich in abundant carrotene and vitamin A in strawberry, yctalopia can be alleviated, have and safeguard epithelium
Tissue health, improving eyesight nourishing the liver, the effect of enhancing development.Rich in abundant dietary fiber in strawberry, the compacted of intestines and stomach can be promoted
It is dynamic, promote the food digestion in intestines and stomach, improve constipation, prevent the generation of acne, intestinal cancer.
Ripe Mulberry fruit（It is purple, red）Nutritious, every 100 grams contain：81.8 grams of moisture, 48 kilocalories of energy, protein 1.6
~ 1.8 grams, 0.3 ~ 0.4 gram of fat.Dietary fiber（Cellulose）3.3 ~ 4.9 grams, 10 ~ 12.9 grams of carbohydrate, 1.2 grams of ash content,
Vitamin A：3 micrograms, the microgram of carrotene 20 ~ 30,0.02 milligram of thiamine, 0.05 ~ 0.06 milligram of riboflavin, vitamin E：
6.95 ~ 12.78 milligrams, 33 milligrams of potassium, 30 milligrams of calcium, P33 milligram, 32 milligrams of potassium, 1.9 milligrams of sodium, 0.3 milligram of iron, zinc 0.25
~ 0.27 milligram, 0.06 ~ 0.08 milligram of copper, the microgram of selenium 4.8 ~ 6.5,0.29 milligram of manganese.In addition, also contain tannic acid, and malic acid, dimension
Raw plain C and aliphatic acid etc..Its fat is mainly linoleic acid, oleic acid, palmitic acid, stearic acid and a small amount of octanoic acid, n-nonanoic acid, capric acid, meat
Myristic acid, leukotrienes etc..Mulberry fruit dietary function：Mulberry fruit is sweet sour, cold in nature, and entering lung, liver, kidney, large intestine channel has liver-kidney tonifying, life
Tianjin ease constipation, UFA eyesight, quench the thirst removing toxic substances, beauty treatment and other effects suitable for deficiency of YIN-blood, have a dizzy spell, night sweat and injury thirst, disappear
Yearningly, the disease such as dry constipation of intestines.
Hawthorn contains a variety of organic acids.Enhancing gastric acidity after oral, improves pepsin activity, promotes disappearing for protein
Change；Hawthorn is sour, stimulates stomach lining to promote gastric secretion；Fatty enzyme in hawthorn, the digestion of fat can be promoted；Hawthorn contains dimension
The compositions such as raw plain C, can orally improve a poor appetite；Hawthorn has adjustment effect to gastrointestinal motility function, and the stomach and intestine of spasticity are put down
Sliding flesh has inhibitory action, has excitation hawthorn to resist with reducing blood lipid, antiatherosclerosis the smooth muscle of relaxed state
Myocardial ischemia, cardiac stimulant, there are certain antihypertensive effect, antioxidation.Hawthorn is uncommon to Shigella, proteus, large intestine angstrom
Bacterium, staphylococcus aureus etc. have stronger bacteriostasis.
Collagen polypeptide it can have improvement to circulate, be favourable to coronary heart disease, ischemic cerebral disease with prevention of cardiovascular disease.Collagen is more
Peptide can encase cancer cell, prevent it and rise in value or shift.It is adapted to the patients with severe symptoms such as diabetes, kidney sufferer to absorb high-quality high protein to protect
Health food.Collagen is maintain joint and bone health optimal calcareous.Collagen polypeptide can improve the puzzlement of gynecological disease.
Contain a large amount of glycine in collagen polypeptide, be not only involved in rubber polymer original in human body, but also it is in one kind in brain cell to be
Pivot nerve inhibitory transmitter, to produce the sedation of Central nervous, there is good treatment to make to anxiety disorder, neurasthenia etc.
With collagen food, protein cohesion caused by hydrochloric acid in gastric juice acts on can be suppressed in stomach, so as to be advantageous to food
The effect of digestion, also gastric acid inhibitory and stomach protoenzyme secretion, can mitigate patients w ith peptic ulcer disease pain, promote gastric ulcer healing.Collagen
Polypeptide is the perceptron for the amoebocyte cleaning foreign matter for being responsible for critical function in body immune effect, therefore to prevention disease very
It is helpful.
Stachyose can effectively prevent and alleviate constipation of infantile and diarrhoea, suppress intestines problem, improve the machine of infant
Body premunition.With gut purge road, the effect of toxin expelling lead discharging.Stachyose can replace metal ion, so as to promote the mineral such as calcium
Matter absorbs.Human body resistance infection of digestive canal or the function by food-borne illness can be strengthened, and the organs such as skin can be improved
Nutrition condition, improve health, beautifying face and moistering lotion.It is particularly suitable for：Functional disturbances of gastrointestinal tract person, intestinal flora imbalance patient, custom
Property constipation or diarrhoea person, enteritis, gastritis, gastric ulcer, often take antibiotic person, old man, the neonate of non-breastfeeding, obesity
Disease patient, yeast infection person etc..
In compound microorganism ferments beverage containing anthocyanidin prepared by the present invention：Lactobacillus plantarum（Lactobacillus
plantarum）CICC20265, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136, acidophilus breast bar
Bacterium（Lactobacillus acidophilus）CICC6083, bifidobacterium breve（Bifidobacterium breve）
CICC6185 belongs to Lactobacillus together, and biological property is similar, and in the case where pH value is 3.0-3.5 acid condition, it can form phase
To stable resting state, each strain is set mutually not restrict counteracting, meanwhile, saccharomyces cerevisiae can protect anthocyanidin not broken
Bad, Lactobacillus rhamnosus promotes the breeding of lactobacillus acidophilus and Bifidobacterium.Multi-cultur es formed symbiosis, play it is leading and
Central role, to ensure that multi-cultur es microbial composite beverage agent activity liquid status provided by the invention are stable, function diversification.
Clinical detection is shown：Taking in medium/high dosage OPC can be to the intestinal inflammatory of mouse, oxidative stress, barrier work(
There can be improvement result.Median dose is about 30mg/kg, and the dosage taken in people from dietary supplements coincide substantially, and high
Dosage（60 mg/kg）More than the intake dosage in dietary supplements.
The mouse big to 36 weeks feeds cyanidin extract 15 weeks, is then randomly divided into 4 groups, i.e. control group（0 mg/
kg）, low dosage（10 mg/kg）, the mg/kg of middle dosage 30 and the mg/kg of high dose 60, be further continued for feeding 3 weeks.Researcher is led to
Cross to myeloperoxidase（MPO）Activity determination finds that control group mouse intestinal inflammatory increases, and cyanidin extract group mouse intestines
Road inflammation substantially reduces.In addition, being compared with control group, there is obvious reduce in the content of active oxygen in experimental group mouse serum.
The function of gut barrier has been also checked in addition, and especially have studied the close connection between enterocyte, simultaneously
For enteric cavity, another side is cell and tissue.In a health system, the material in enteric cavity can enter tissue by cell, and
Control the entrance of material.In a unsound system, close connection is broken down, and material can get around cell in enteric cavity,
By closely connecting into tissue, this can increase intestinal permeability, that is, it has often been said that intestines leakage disease.
Whether there is the infiltrative effect of regulation gut barrier to assess to anthocyanidin, gut barrier infiltration can cause intestines
The appearance of road inflammation.As a result showing, claudin-3 white matter and fat and triglycerides cyclical level are negatively correlated, although two
Without direct causality between person, but the also potential expression for implying fat increase and reducing close coordinator, this and people
The hypothesis that intestinal permeability can be increased fat and high fat diet is basically identical.In view of the important of healthy gut barrier function
Property, Dietary frequency can be by way of adjusting intestinal permeability, and then prevents and alleviate fat related metabolic disease.
Generally speaking, oral procyanidin extract can adjust intestinal inflammatory, oxidative stress, improve gut barrier function.
Based on the studies above result, under the background of obesity, people should widely probe into nutrition or Dietary frequency therapy is good for enteron aisle
Health and the effect of gut permeability regulation.
Beneficial effects of the present invention：The present invention is prepared using multiple beneficial microbial strains, multi-cultur es microbial fermentation
Purple sweetpotato, grape, blueberry, strawberry, mulberries, hawthorn beverage preparation containing anthocyanidin, can not only be abundant after human body is entered
Play respective function, promote the propagation of internal beneficial bacterium, suppress the breeding of harmful bacteria, additionally it is possible to for other human bodies beneficial to micro- life
Material necessary to the growth of thing and Function provide（Including growth factor, carbohydrate, amino acid etc.）, adjust human gastrointestinal tract and
Microecological balance in reproductive system, human body is in microecological balance, whet the appetite, promote digestion, while prevent various stomaches
Pole chronic disease in enteron aisle, remove free radical, anti-oxidant reach cosmetic result.Purple sweetpotato, grape, blueberry, grass in preparation
The certain kind of berries, mulberries, hawthorn, collagen polypeptide, the medicine of stachyose eat special value in the presence of probiotics, are more preferably rapidly absorbed by a human and sharp
With.
Contain substantial amounts of anthocyanidin, effect of the anthocyanidin to human body in beverage prepared by the present invention：Scavenger free radical,
Scavenging activity is ascorbic 20 times, 50 times of vitamin E, can improve immunity with human activin immune system.Accelerate eye
Portion's blood circulation, visual purple regeneration, resists eye strain, strengthens eyesight, extremely effective while right for pre- myopic-preventing generation
There is extremely strong eye health-care effect in computer work crowd.Cerebral central nervous system can directly be protected, prevent stress reaction and
Platelet aggregation caused by smoking reduces the generation of heart disease and stroke.Strengthen blood vessel elasticity, improve the circulatory system and enhancement
The smoothness of skin, blood glucose is reconciled, suppress inflammation and allergy, improve the pliability in joint.
If infectious diseases system of California, USA university professor Buddhist doctor of Western medicine thinks that anthocyanidin totally nontoxic, experiment proves flower
Blue or green element can make normal person's function of immune system lift 50%, make patient's immunologic function lifting 300 that immunologic function declines to a great extent~
700%。
The present invention carries out drink fermented using microbial bacteria, produces a large amount of benefit materials such as B family vitamin and amino acid, makes enteron aisle
The composition of flora occurs, beneficial to change, to improve human gastrointestinal tract function, recovers colony balance in human body intestinal canal, forms antibacterial biological
Barrier, safeguard health；Suppress the breeding of spoilage organisms, clear up toxin caused by spoilage organisms, remove enteron aisle rubbish.Suppress courage to consolidate
Alcohol absorbs, reducing blood lipid, hypotensive activity；Immunoregulation effect, strengthen body immunity and resistance；Antitumor, pre- anti-cancer
Effect；SOD enzyme activities are improved, eliminate human free radical, tool anti-aging, effect of promoting longevity.
In prepared beverage, viable bacteria may adhere to intestinal wall after entering human body（Also referred to as it is colonized）, so account at " seat "
The living space of harmful bacteria is repelled；Beneficial bacterium is by growth and breeding, caused lactic acid and acetic acid, reduces the PH and Eh of enteron aisle
Value, improve internal microenvironment, the growth of harmful bacteria can be suppressed；Its metabolite is to the nutritious effect of human body；Beneficial bacterium has promotion
The effect of immune function of human body.Therefore it is obvious the effect of viable bacteria.
Embodiment
With reference to specific examples below, the present invention is described in further detail, but the present invention is not limited solely to
Following examples.
Embodiment 1：A kind of compound microorganism ferments beverage containing anthocyanidin, including following components and each component ratio be：It is purple
Sweet potato 5%, grape 10%, blueberry 10%, strawberry 10%, mulberry fruit 10%, hawthorn 5%, collagen polypeptide 1%, microbe leaven 0.1%, water
Threose 0.5%, water 48.4%；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）
CICC20265 zymotic fluids, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 zymotic fluids, acidophilus breast
Bacillus（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve（Bifidobacterium
breve）CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 zymotic fluids are according to weight
Than for 1:1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml。
The method for preparing above-mentioned compound microorganism ferments beverage containing anthocyanidin, is comprised the following steps that：
（1）Fruit pre-processes：Purple sweetpotato, grape, blueberry, strawberry, mulberry fruit, hawthorn are cleaned respectively, crushed after mixing in proportion,
Then plus fruit juice liquid medium is made in water, standby after sterilizing；
（2）The preparation of microbe leaven：The preparation of zymotic fluid：Zymotic fluid culture medium prescription is：Peptone 10.1g, beef extract
10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, distilled water 1.0L, by Lactobacillus plantarum（Lactobacillus
plantarum）CICC20265, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136, lactobacillus acidophilus
（Lactobacillus acidophilus）CICC6083, bifidobacterium breve（Bifidobacterium breve）
CICC6185, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 is aseptically inoculated with into fermentation respectively
In liquid culture medium, 28 DEG C~32 DEG C single Spawn incubations 24~48 hours, obtain respectively Lactobacillus plantarum bacterium number >=5.0 ×
109Cfu/ml zymotic fluid, Lactobacillus rhamnosus bacterium number >=5.0 × 109Cfu/ml zymotic fluid, lactobacillus acidophilus bacterium number >=5.0
×109Cfu/ml zymotic fluid, bifidobacterium breve bacterium number >=5.0 × 109Cfu/ml zymotic fluid, saccharomyces cerevisiae bacterium number >=5.0 ×
109Cfu/ml zymotic fluid；By single strain fermentating liquid of acquisition according to weight ratio be 1:1:1:1：1 ratio mixing is micro- life
Thing leavening；
（3）Drink fermented：In the fermentation tank to have sterilized, by step（1）0.1% is added in the fruit juice liquid medium to have sterilized
Microbe leaven, the volume ratio of the zymotic fluid in the volume and fermentation tank that oxygen dissolved is oxygen in 5% i.e. fermentation tank is 5%
Under the conditions of, 35 DEG C~37 DEG C closed quiescent cultures 72 hours, pH value drops to 3.0~3.5 and produces complex microorganism hair in fermentation tank
Ferment beverage containing anthocyanidin.Anthocyanidin content is 100mg/500g in the compound microorganism ferments beverage containing anthocyanidin.
Embodiment 2：A kind of compound microorganism ferments beverage containing anthocyanidin, including following components and each component ratio be：It is purple
Sweet potato 10%, grape 5%, blueberry 10%, strawberry 10%, mulberry fruit 5%, hawthorn 10%, collagen polypeptide 1%, microbe leaven 0.1%, water
Threose 0.5%, water 48.4%；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）
CICC20265 zymotic fluids, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 zymotic fluids, acidophilus breast
Bacillus（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve（Bifidobacterium
breve）CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 zymotic fluids are according to weight
Than for 1:1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml.Its
Preparation method is the same as preparation method described in embodiment 1.Anthocyanidin content is in the compound microorganism ferments beverage containing anthocyanidin
100mg/500g。
Embodiment 3：A kind of compound microorganism ferments beverage containing anthocyanidin, including following components and each component ratio be：It is purple
Sweet potato 5%, grape 10%, blueberry 5%, strawberry 5%, mulberry fruit 10%, hawthorn 5%, collagen polypeptide 1%, microbe leaven 0.1%, wood
Sugar 0.5%, water 58.4%；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）
CICC20265 zymotic fluids, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 zymotic fluids, acidophilus breast
Bacillus（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve（Bifidobacterium
breve）CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 zymotic fluids are according to weight
Than for 1:1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml.Its
Preparation method is the same as preparation method described in embodiment 1.Anthocyanidin content is in the compound microorganism ferments beverage containing anthocyanidin
100mg/500g。
Embodiment 4：A kind of compound microorganism ferments beverage containing anthocyanidin, including following components and each component ratio be：It is purple
Sweet potato 7%, grape 8%, blueberry 6%, strawberry 9%, mulberry fruit 7%, hawthorn 8%, collagen polypeptide 1%, microbe leaven 0.1%, stachyose
0.5%, water 53.4%；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）CICC20265
Zymotic fluid, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 zymotic fluids, lactobacillus acidophilus
（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve（Bifidobacterium breve）
CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 zymotic fluids are 1 according to weight ratio:
1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml.Its
Preparation method is the same as preparation method described in embodiment 1.Anthocyanidin content is in the compound microorganism ferments beverage containing anthocyanidin
100mg/500g。
Embodiment 5：A kind of compound microorganism ferments beverage containing anthocyanidin, including following components and each component ratio be：It is purple
Sweet potato 5%, grape 7%, blueberry 8%, strawberry 10%, mulberry fruit 6%, hawthorn 5%, collagen polypeptide 1%, microbe leaven 0.1%, stachyose
0.5%, water 57.4%；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）CICC20265
Zymotic fluid, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 zymotic fluids, lactobacillus acidophilus
（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve（Bifidobacterium breve）
CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 zymotic fluids are 1 according to weight ratio:
1:1:1：1 ratio mixes.
The Lactobacillus plantarum（Lactobacillus plantarum）Bacterium number >=5.0 of CICC20265 zymotic fluids ×
109Cfu/ml, Lactobacillus rhamnosus（Lactobacillus rhamnosus）Bacterium number >=5.0 of CICC6136 zymotic fluids ×
109Cfu/ml, lactobacillus acidophilus（Lactobacillus acidophilus）Bacterium number >=5.0 of CICC6083 zymotic fluids ×
109Cfu/ml, bifidobacterium breve（Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/
ml；Saccharomyces cerevisiae（Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml.Its
Preparation method is the same as preparation method described in embodiment 1.Anthocyanidin content is in the compound microorganism ferments beverage containing anthocyanidin
100mg/500g。
Claims (4)
- A kind of 1. compound microorganism ferments beverage containing anthocyanidin, it is characterised in that：It is including following components and each component ratio：It is purple Sweet potato 5-10%, grape 5-10%, blueberry 5-10%, strawberry 5-10%, mulberry fruit 5-10%, hawthorn 5-10%, collagen polypeptide 1%, microorganism Leavening 0.1%, stachyose 0.5%, remaining is water；The microbe leaven is：Lactobacillus plantarum（Lactobacillus plantarum）CICC20265 zymotic fluids, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136 ferments Liquid, lactobacillus acidophilus（Lactobacillus acidophilus）CICC6083 zymotic fluids, bifidobacterium breve （Bifidobacterium breve）CICC6185 zymotic fluids, saccharomyces cerevisiae（Saccharomyces cerevisiae） CICC1624 zymotic fluids are 1 according to weight ratio:1:1:1：1 ratio mixes.
- A kind of 2. compound microorganism ferments beverage containing anthocyanidin according to claim 1, it is characterised in that：The plant breast Bacillus（Lactobacillus plantarum）Bacterium number >=5.0 × 10 of CICC20265 zymotic fluids9Cfu/ml, rhamnose breast bar Bacterium（Lactobacillus rhamnosus）Bacterium number >=5.0 × 10 of CICC6136 zymotic fluids9Cfu/ml, lactobacillus acidophilus （Lactobacillus acidophilus）Bacterium number >=5.0 × 10 of CICC6083 zymotic fluids9Cfu/ml, bifidobacterium breve （Bifidobacterium breve）Bacterium number >=5.0 × 10 of CICC6185 zymotic fluids9cfu/ml；Saccharomyces cerevisiae （Saccharomyces cerevisiae）Bacterium number >=5.0 × 10 of CICC1624 zymotic fluids9cfu/ml。
- 3. preparing a kind of method of compound microorganism ferments beverage containing anthocyanidin described in claim 1 or claim 2, it is special Sign is：Comprise the following steps that：（1）Fruit pre-processes：Purple sweetpotato, grape, blueberry, strawberry, mulberry fruit, hawthorn are cleaned respectively, crushed after mixing in proportion, Then plus fruit juice liquid medium is made in water, standby after sterilizing；（2）The preparation of microbe leaven：The preparation of zymotic fluid：Zymotic fluid culture medium prescription is：Peptone 10.1g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, distilled water 1.0L, by Lactobacillus plantarum（Lactobacillus plantarum）CICC20265, Lactobacillus rhamnosus（Lactobacillus rhamnosus）CICC6136, lactobacillus acidophilus （Lactobacillus acidophilus）CICC6083, bifidobacterium breve（Bifidobacterium breve） CICC6185, saccharomyces cerevisiae（Saccharomyces cerevisiae）CICC1624 is aseptically inoculated with into fermentation respectively In liquid culture medium, 28 DEG C~32 DEG C single Spawn incubations 24~48 hours, obtain respectively Lactobacillus plantarum bacterium number >=5.0 × 109Cfu/ml zymotic fluid, Lactobacillus rhamnosus bacterium number >=5.0 × 109Cfu/ml zymotic fluid, lactobacillus acidophilus bacterium number >=5.0 ×109Cfu/ml zymotic fluid, bifidobacterium breve bacterium number >=5.0 × 109Cfu/ml zymotic fluid, saccharomyces cerevisiae bacterium number >=5.0 × 109Cfu/ml zymotic fluid；By single strain fermentating liquid of acquisition according to weight ratio be 1:1:1:1：1 ratio mixing is micro- life Thing leavening；（3）Drink fermented：In the fermentation tank to have sterilized, by step（1）0.1% is added in the fruit juice liquid medium to have sterilized Microbe leaven, the volume ratio of the zymotic fluid in the volume and fermentation tank that oxygen dissolved is oxygen in 5% i.e. fermentation tank is 5% Under the conditions of, 35 DEG C~37 DEG C closed quiescent cultures 72 hours, pH value drops to 3.0~3.5 and produces complex microorganism hair in fermentation tank Ferment beverage containing anthocyanidin.
- 4. according to claim 3 prepare a kind of method of compound microorganism ferments beverage containing anthocyanidin, it is characterised in that： Anthocyanidin content is 100mg/500g in the compound microorganism ferments beverage containing anthocyanidin.
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