CN103431467B - Asparagus extract bifidobacterium fermentation activity beverage and preparation method thereof - Google Patents
Asparagus extract bifidobacterium fermentation activity beverage and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an asparagus extract bifidobacterium fermentation activity beverage and a preparation method thereof. An asparagus extract, brown sugar and stachyose serve as a culture medium; the following strains as shown in the specification are subjected to anaerobic fermentation to culture a bifidobacterium; after the anaerobic fermentation, a fermented probiotic liquid is subjected to sterile filling; and the bifidobacterium, lactobacillus plantarum, lactobacillus acidophilus and saccharomyces cerevisiae are freeze-dried bacteria bought from a culture collection center. The beverage supplements a specific bifidus factor and an exogenous bifidus factor in a plant in order to culture high-density bifidobacterium longum, increase the unit bacterial count of other lactic acid bacteria, conduct enzymolysis of small protein converting peptide in the culture medium, increase the content of amino acid essential for a human body, and improve the activity of asparagus juice, and a health care function of the whole fermentation liquid. The technology has the advantages that a strict anaerobic condition of the bifidobacterium is overcome, an expensive bifidobacterium fermentation device is removed, and the health care function of metabolic products of an active substance and a microorganism containing asparagus, as well as the fermentation liquid of the viable bacteria is enhanced.
Description
Technical field: the preparation method that the present invention relates to the two qi probiotics fermention active beverage of a kind of Germinatus Phragmitis extract.
Background technology
Probiotic beverage, is commercially most favored by customers, has the supping of abundant nutrition and medicinal function.But, the probiotic beverage in current supply market, functional not strong, the use of the additives such as artificial color and seasoning essence, thickener, anticorrisive agent, more make the beverage of existing listing, there is many unhealthful unsafe factors and single most of ox milk drink of struggling, have fermented beverage to be also deactivation, the viable bacteria beverage really with health care is little.The deep level development that the present invention carries out with regard to health-care effect and the function of beverage utilizes.
Asparagus English name: Cacumen Asparagi (drawing); Asparagus defines: the tender stem of liliaceous plant asparagus Asparagus officinal is L..Application branch of learning traditional Chinese medicine and pharmacy (first level discipline); Chinese pharmacology (secondary subject); Chinese medicine (secondary subject), asparagus is one of large famous dish in the world ten, has another name called asparagus.Enjoy the laudatory title of " kings of vegetables " in the international market, asparagus is rich in several amino acids, protein and vitamin, its content is all higher than general fruits and vegetables, asparagine particularly in asparagus and trace elements of selenium, molybdenum, chromium, manganese etc., there is adjustment organism metabolism, improve effect of body immunity, in the prevention and therapy to hypertension, heart disease, leukaemia, leukemia, oedema, cystitis etc., there is very strong inhibitory action and pharmacodynamics effect.Stachyose English name: stachyose; Lupeose defines: " the non-reduced tetrose be made up of galactolipin, glucose and fructose.Its structural formula is: D-galactopyranosyl glycosyl α-1
Application branch of learning: 6-D-glycopyranosyl α-1 (first level discipline); 2-beta-D-fructofuranose glycosides, belongs to the derivative products of sucrose, is the homologue of gossypose, with α-1 (secondary subject) on the galactolipin C-6 position hydroxyl of gossypose; 6-glycosidic bond connects a galactolipin again, is distributed widely in plant." (secondary subject) stachyose is naturally occurring a kind of tetrose, is a kind of functional oligose that significantly can promote the beneficial bacterium propagation such as Bifidobacterium.Sterling is white powder, and taste is slightly sweet, and sugariness is 22% of sucrose, good taste, without any bad mouthfeel or peculiar smell.Its molecular structure: " galactolipin-galactolipin-Glucose-Fructose ", molecular formula is C24H42021, relative molecular weight 666.59.
Stachyose has extremely significantly proliferation function to profitable strains such as the Bifidobacterium in human gastrointestinal tract, Bacillus acidi lacticis, can improve rapidly human body alimentary canal environment, regulates Tiny ecosystem colony balance.It can promote to form the dominant bacteria status of beneficial bacterium in alimentary canal, aerogenesis is suppressed to produce the production of the spoilage organisms such as sour clostridium, produce a large amount of physiological activator in addition, regulating intestinal canal pH value, kill pathogenic bacteria, check spoilage product to generate, suppress endogenous carcinogenic generation and absorption, and decomposition derives Multiple immunizations function factor.Be commonly called as: the food of beneficial bacterium.
1. stachyose is similar to some water-soluble dietary fiber, nontoxic, direct-edible.
2. stachyose has extremely significantly proliferation function to profitable strains such as the Bifidobacterium in human gastrointestinal tract, Bacillus acidi lacticis, can improve rapidly human body alimentary canal environment.
3. stachyose is not destroyed in the organ such as stomach, small intestine of human body, directly can arrive the large intestine position at intestinal beneficial flora place, significantly promotes Bifidobacterium propagation.
4. stachyose can promote the synthesis of the vitamins such as B1, B2, B6, B12, promotes that intestines and stomach are to calcium, the absorption of the elements such as magnesium.
5. stachyose is not the material of intestinal tympanites effect, does not produce abdominal distension after taking, and quietly moistens intestines and stomach.
6. stachyose has active factors, adsorbable intestines and stomach noxious material and pathogen, and then strengthens body immunity.
Summary of the invention:
The object of the invention is to provide a kind of two qi probiotics fermention active beverage of Germinatus Phragmitis extract increasing the amino acid content of needed by human.The present invention is able to an object and is to provide the two qi probiotics fermention active bacterial beverage preparation method of a kind of Germinatus Phragmitis extract: the symphysis metamember perfect adaptation that probio and medicinal asparagus are contained also has been carried out probiotics fermention experiment, strengthens human body disease-proof functions.
Technical scheme of the present invention is the two qi probiotics fermention active bacterial beverage of a kind of Germinatus Phragmitis extract, adopt Germinatus Phragmitis extract, brown sugar, stachyose, as culture medium, uses following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Addicted to yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Plant lactobacillus bacterium number: CCCi0141, Lactobacillusplantarum (Orla-Jehsen) Holland; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation produces is carried out sterile filling, described Bifidobacterium, plant lactobacillus, addicted to yogurt bacterium, addicted to yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum bought from DSMZ, each bacterial classification mixing quality percentage is: 25%, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1; Described Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, Germinatus Phragmitis extract to be active ingredient be 10: 1 extract, stachyose Oilgosaccharkdes is buying from market of 90% content, Germinatus Phragmitis extract: active ingredient is the extract of 10: 1, stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
The technical scheme of the two qi probiotics fermention active bacterial beverage preparation method of Germinatus Phragmitis extract of the present invention is: A: fermented asparagus juice
preparation method;
1, asparagus juice and Oilgosaccharkdes and other auxiliary materials is prepared
The asparagus juice got ready and Oilgosaccharkdes and other auxiliary materials are added fermentation tank, regulates acid-base value with sodium carbonate, make pH value reach 6.4;
2, raw and auxiliary material is carried out heat sterilization, temperature 121 DEG C, sterilizing half an hour, and be cooled to rapidly 40 degree;
3, bacterium is connect by sterile working
Heat-preservation fermentation 40 DEG C fermentation 30 hours, PH4-4.5, lactic acid 0.85-1%, terminate fermentation;
4, product index yellowish-brown or light yellow muddy state, have the delicate fragrance of asparagus and lactic acid fermented fragrance, sour and sweet palatability, pH value 4-4.5, lactic acid percentage composition 0.85-1%, lactic acid bacteria 10
8/ ml soluble solid " 3%, containing vitamin C 14.52mg calcium 41-44mg in every 100ml fermenation raw liquid;
5, detect without pathogenic bacteria, Escherichia coli quantity is lower than the standard of country, and it is qualified to be considered as;
6 stostes that will ferment, carry out sterile filling;
7 finished product packing warehouse-ins;
B: get the finished product Germinatus Phragmitis extract stoste prepared by steps A and brown sugar, stachyose is by stoste 10%, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into fermentation tank sterilizing and terminates for 1 hour, be down to 35 DEG C when temperature and add mixed culture fermentation agent hybrid anaerobic fermentation, leavening is mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. is addicted to yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: 25%: 25%: 25%: 25%, namely 1: 1: 1: 1, the consumption of mixed culture fermentation agent: 1-10 ‰, wherein each bacterial classification content reaches following requirement had both been qualified products: Bifidobacterium>=10
7cfu/g, plants lactobacillus>=10
7cfu/g, addicted to yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank controls constant temperature 35 DEG C, continuing fermentation 48-72 hour, stops fermentation when pH value is 3.5-4, and detect miscellaneous bacteria number, qualified zymotic fluid is carried out sterile filling, and package test obtains finished product.
As shown in Figure 1, detect amino acid whose content in the asparagus juice without fermentation and fermentation, except methionine, have the content of 6 seed amino acids to increase all to some extent, increase by 46.9% altogether, nutriment increases considerably.
As shown in Figure 2 without the content of delicious amino acid in the asparagus juice fermented and ferment, detect, glutamic acid increases by 17%, and asparatate increases by 32%, so be that mouthfeel and taste change all to some extent.
The present invention has the following advantages:
1, principle of the present invention; Use without antagonistic microbe intergrowth theory, aerobic bacteria air takes oxygen by force, for the anaerobic bacteria that condition is strict creates good growing environment, supplementing distinctive Oilgosaccharkdes and foreign aid's Oilgosaccharkdes in plant is for cultivating high density bifidobacterium longum, also allow other lactic acid bacteria improve unit bacterium number, the albumen in enzymolysis culture medium transforms little peptide, increases the amino acid content of needed by human, improve the activity of asparagus juice, and the health care of the zymotic fluid of entirety.
2 technological advantages overcome the strict anaerobic condition of Bifidobacterium, make good use of oxygen animalcule, carry out air and take oxygen by force, eliminate the installation for fermenting of expensive Bifidobacterium, the industrialization making Bifidobacterium common is produced, optimize culture medium and cultivate bar condition, the metabolite of the active material containing asparagus and microorganism and the zymotic fluid health care of viable bacteria are strengthened.
3 with dry Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, as Bifidobacterium, plants lactobacillus, fermentation substrate matter addicted to the probio of the symbiosis such as yogurt bacterium, saccharomycete, has widened further and added the functional of asparagus.
4 do culture medium with Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, cultivate Bifidobacterium, plant lactobacillus, addicted to probios such as yogurt bacterium, Lactobacillus brevis, saccharomycete, not only increase the health care of asparagus in nutrient solution, under this special fermentation substrate environment, the high-activity probiotics active bacteria formulation cultivated, can also activity and the property of medicine improving the effective health-care components of merit asparagus.
5 provide Research idea for the probiotics preparation and fermented beverage researching and developing a kind of Chinese medicine asparagus of novel medicine-food two-purpose further; Utilize the active ingredient of asparagus to provide sufficient nutrition for growth of probiotics, probio makes again the drug effect of asparagus be improved.
6 raw material sources of the present invention enrich, with low cost;
7 stable preparation process of the present invention, investment of production equipment is less, simple to operate, just with industrialization;
The present invention, making every effort to explore the existing making functional beverage of solution, the drawback aspect existed, after use Germinatus Phragmitis extract and stachyose Oilgosaccharkdes do the fermentation substrate of fermented beverage, to the functional enhancing that human body is prevented and cured diseases, be the symphysis metamember perfect adaptation that contains of probio and medicinal asparagus and carried out probiotics fermention experiment, illustrate that disease-proof functions strengthens, the further deep level development carrying out probiotic fermentation beverage utilizes scope.
Principle of the present invention; Use without antagonistic microbe intergrowth theory, aerobic bacteria air takes oxygen by force, for the anaerobic bacteria that condition is strict creates good growing environment, supplementing distinctive Oilgosaccharkdes and foreign aid's Oilgosaccharkdes in plant is for cultivating high density bifidobacterium longum, also other lactic acid bacteria is allowed to improve unit bacterium number, albumen in enzymolysis culture medium transforms little peptide, improves the property of medicine of asparagus, and the health care of the zymotic fluid of entirety.
This technological advantage overcomes the strict anaerobic condition of Bifidobacterium, make good use of oxygen animalcule, carry out air and take oxygen by force, eliminate the installation for fermenting of expensive Bifidobacterium, the industrialization making Bifidobacterium common is produced, optimize culture medium and cultivate bar condition, the metabolite of the active material containing asparagus and microorganism and the zymotic fluid health care of viable bacteria are strengthened.
Accompanying drawing illustrates:
Fig. 1 is that fermentation is shown with the amino acid content (mg/100ml) of needed by human in unfermentable asparagus juice
Fig. 2 is that fermentation is shown with the content (mg/100ml) of unfermentable asparagus juice delicious amino acid
Concrete embodiment
The two qi probiotics fermention active bacterial beverage of a kind of Germinatus Phragmitis extract, adopts Germinatus Phragmitis extract, brown sugar, and stachyose, as culture medium, uses following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Addicted to yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Plant lactobacillus bacterium number: CCC10141, Lactobacillusplantarum (Orla-Jehsen) Holland; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation produces is carried out sterile filling, described Bifidobacterium, plant lactobacillus, addicted to yogurt bacterium, addicted to yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum bought from DSMZ, each bacterial classification mixing quality percentage is: 25%, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1; Described Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, Germinatus Phragmitis extract to be active ingredient be 10: 1 extract, stachyose Oilgosaccharkdes is buying from market of 90% content, Germinatus Phragmitis extract: active ingredient is the extract of 10: 1, stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
The two qi probiotics fermention active bacterial beverage preparation method of Germinatus Phragmitis extract:
A. fermented asparagus juice
preparation method;
1, asparagus juice and Oilgosaccharkdes and other auxiliary materials is prepared
The asparagus juice got ready and Oilgosaccharkdes and other auxiliary materials are added fermentation tank, regulates acid-base value with sodium carbonate, make pH value reach 6.4;
2, raw and auxiliary material is carried out heat sterilization, temperature 121 DEG C, sterilizing half an hour, and be cooled to rapidly 40 degree;
3, bacterium is connect by sterile working
Heat-preservation fermentation 40 DEG C fermentation 30 hours, PH4-4.5, lactic acid 0.85-1%, terminate fermentation;
4, product index yellowish-brown or light yellow muddy state, have the delicate fragrance of asparagus and lactic acid fermented fragrance, sour and sweet palatability, pH value 4-4.5, lactic acid percentage composition 0.85-1%, lactic acid bacteria 10
8/ ml soluble solid " 3%, containing vitamin C 14.52mg calcium 41-44mg in every 100ml fermenation raw liquid;
5, detect without pathogenic bacteria, Escherichia coli quantity is lower than the standard of country, and it is qualified to be considered as;
6 stostes that will ferment, carry out sterile filling;
7 finished product packing warehouse-ins;
B: get the finished product Germinatus Phragmitis extract stoste prepared by steps A and brown sugar, stachyose is by stoste 10%, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into fermentation tank sterilizing and terminates for 1 hour, be down to 35 DEG C when temperature and add mixed culture fermentation agent hybrid anaerobic fermentation, leavening is mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. is addicted to yogurt bacterium ACCC10637, plant lactobacillus ACCCi0141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: 25%: 25%: 25%: 25%, namely 1: 1: 1: 1, the consumption of mixed culture fermentation agent: 1-10 ‰, wherein each bacterial classification content reaches following requirement had both been qualified products: Bifidobacterium>=10
7cfu/g, plants lactobacillus>=10
7cfu/g, addicted to yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank controls constant temperature 35 DEG C, continuing fermentation 48-72 hour, stops fermentation when pH value is 3.5-4, and detect miscellaneous bacteria number, qualified zymotic fluid is carried out sterile filling, and package test obtains finished product.
Embodiment 1
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 10% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into fermentation tank sterilizing and to terminate half an hour, be down to 35 DEG C when temperature and add mixed culture fermentation agent hybrid anaerobic fermentation, leavening is mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. is addicted to yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement had both been qualified products: Bifidobacterium>=10
7cfu/g, plants lactobacillus>=10
7cfu/g, addicted to yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank controls constant temperature 35 DEG C, continuing fermentation 48-72 hour, stops fermentation when pH value is 3.5-4, and detect miscellaneous bacteria number, qualified zymotic fluid is carried out sterile filling, and package test obtains finished product.
Embodiment 2
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 20% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 78% pumps into fermentation tank sterilizing and to terminate half an hour, be down to 35 DEG C when temperature and add mixed culture fermentation agent hybrid anaerobic fermentation, leavening is mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. is addicted to yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement had both been qualified products: Bifidobacterium>=10
7cfu/g, plants lactobacillus>=10
7cfu/g, addicted to yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank controls constant temperature 35 DEG C, continuing fermentation 48-72 hour, stops fermentation when pH value is 3.5-4, and detect miscellaneous bacteria number, qualified zymotic fluid is carried out sterile filling, and package test obtains finished product.
Embodiment 3
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 30% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 68% pumps into fermentation tank sterilizing and to terminate half an hour, be down to 35 DEG C when temperature and add mixed culture fermentation agent hybrid anaerobic fermentation, leavening is mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. is addicted to yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement had both been qualified products: Bifidobacterium>=10
7cfu/g, plants lactobacillus>=10
7cfu/g, addicted to yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank controls constant temperature 35 DEG C, continuing fermentation 48-72 hour, stops fermentation when pH value is 3.5-4, and detect miscellaneous bacteria number, qualified zymotic fluid is carried out sterile filling, and package test obtains finished product.
Claims (1)
1. the two qi probiotics fermention active bacterial beverage of Germinatus Phragmitis extract, it is characterized in that, adopt Germinatus Phragmitis extract, brown sugar, stachyose Oilgosaccharkdes, as culture medium, uses following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Addicted to yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Lactobacillus plantarum bacterium number: CCC10141, Lactobacillusplantarum (Orla-Jehsen) Ho1land; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation produces is carried out sterile filling, described bifidobacterium longum, Lactobacillus plantarum, addicted to yogurt bacterium, addicted to yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum bought from DSMZ, each bacterial classification mixing quality percentage is: respectively account for 25%, namely 1: 1: 1: 1; Germinatus Phragmitis extract: active ingredient is the extract of 10: 1, stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
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| CN104187930B (en) * | 2014-07-29 | 2017-01-18 | 四川农业大学 | Bitter bamboo shoot juice drink and preparation method thereof |
| CN104224852A (en) * | 2014-09-11 | 2014-12-24 | 领绿生物镇江有限公司 | Efficient composite micro-ecological preparation and preparation method thereof |
| CN104489092A (en) * | 2014-12-01 | 2015-04-08 | 于力欣 | Health drink containing grosvener siraitia extract and active bifidobacterium longum and preparation method for health drink containing grosvener siraitia extract and active bifidobacterium longum |
| CN104957617A (en) * | 2015-06-30 | 2015-10-07 | 钟训艳 | Stachys probiotic composition |
| CN106036318B (en) * | 2016-06-12 | 2019-11-12 | 中国食品发酵工业研究院有限公司 | A kind of asparagus fermented beverage and preparation method thereof that can improve function of intestinal canal |
| CN107348276B (en) * | 2017-07-24 | 2021-05-18 | 秦皇岛长胜营养健康科技有限公司 | Method for improving bioavailability of concentrated asparagus juice, asparagus drink and application |
| CN107373265A (en) * | 2017-09-15 | 2017-11-24 | 北京百丰天下生物科技有限公司 | Concentrate viable bacteria quantum beverage and preparation method thereof |
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