CN105815641A - Preparation method of comprehensive yeast liquid with anti-tumor activity and application of comprehensive yeast liquid - Google Patents
Preparation method of comprehensive yeast liquid with anti-tumor activity and application of comprehensive yeast liquid Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/157—Lactis
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the field of food engineering, in particular to a preparation method of a comprehensive yeast liquid with anti-tumor activity and application of the comprehensive yeast liquid. The preparation method mainly comprises the following steps: pretreating raw materials, performing enzymolysis, filtering, adding dried lucid ganoderma powder for fermentation, performing acetic fermentation, filtering and sterilizing, and performing sterile filling. The preparation method of the comprehensive yeast liquid with anti-tumor activity, which is provided by the invention, is simple in operation step, wide in raw material source, rich and comprehensive in nutrition as multiple vegetables, fruits and bacteria are matched and combined, very good in taste and applicable to daily drinking as the components are reasonably proportioned and matched, and capable of inhibiting invisible tumor cell growth in human bodies, improving the immunity and promoting body health.
Description
Technical field
The present invention relates to food engineering field, particularly relate to the preparation method and its usage of a kind of comprehensive enzyme liquid with anti-tumor activity.
Background technology
Ferment is with one or more fresh water fruits and vegetables, mushroom, Chinese herbal medicine etc. as raw material, through the functional fermented product that multiple-microorganism fermentation produces, it not only remains material intrinsic in raw material, also create and multiple there is bioactive microbial secondary metabolite, containing nutritional labelings such as aminoacid, vitamin, enzyme and mineral, it is the material bases depended on for existence of people.Ferment has antibacterial action, antiinflammation, decomposition, antioxidation, can purify blood, promote hyperplasia, can clear up again intestinal, to improving immunity of organisms, maintaining health to have important function.
Current increasing environmental pollution, social competition is fierce, the life stress of people increases the most therewith, activity in vivo enzyme is incremented by with the age and gradually decreases, healthy also can be on the hazard therewith, tumor cell is also likely to spread the most in vivo accumulation, to this end, we must supplement the nutrient substance of general equilibrium in time.But, food is through adding hot-working, and a lot of active substances suffer high temperature, air oxidation.
Summary of the invention
The purpose of the present invention is i.e. the shortcoming overcoming prior art, it is provided that the preparation method and its usage of a kind of comprehensive enzyme liquid with anti-tumor activity.
The present invention adopts the following technical scheme that:
The preparation method of a kind of comprehensive enzyme liquid with anti-tumor activity, comprises the steps:
Step one, cleans pears, Fructus Mali pumilae, Fructus Fici, Rhizoma Zingiberis Recens, Rhizoma Dioscoreae and Fructus Lycopersici esculenti, smash, mixes；
Step 2, adding the cellulase of 0.05～0.1wt% in the slurry of mixing and the pectase of 0.05～0.1wt% carries out enzymolysis, the α-amylase of the protease and 0.1wt% that are added followed by lipase, 0.01～the 0.02wt% of 0.01～0.02wt% carries out enzymolysis；
Step 3, is placed in enzymolysis solution in sealed fermenting tank, adds the sucrose of 25wt%, adds yeast and lactic acid bacteria, mix homogeneously, ferment 60 days, by fermentation liquid sedimentation, filter and remove residue；
Step 4, adds dry Ganoderma powder in fermentation liquid, carries out ferment in second time, ferment 60 days after mixing；Add acetic acid bacteria, anaerobic fermentation 40～60 days afterwards；
Step 5, the fermentation liquid obtained is carried out membrane filtration degerming, 75 DEG C maintain 20 minutes sterilization, sterile filling.
The parts by weight of described pears, Fructus Mali pumilae, Fructus Fici, Rhizoma Zingiberis Recens, Rhizoma Dioscoreae, Fructus Lycopersici esculenti, dry Ganoderma powder and sucrose are: pears 16～20 parts, Fructus Mali pumilae 16～20 parts, Fructus Fici 10～14 parts, Rhizoma Zingiberis Recens 2～4 parts, Rhizoma Dioscoreae 10～14 parts, Fructus Lycopersici esculenti 16～18 parts, dry Ganoderma powder 18～22 parts, sucrose 20～30 parts.
In described step 2, the hydrolysis pH scope of cellulase and pectase is 4.0～5.5, and temperature range is 40～50 DEG C, enzymolysis 5～10 hours；The hydrolysis pH scope of lipase, protease and α-amylase is 6.0～7.5, and temperature range is 35～45 DEG C, enzymolysis 3～7 hours.
Described yeast is yeast Kluyveromyces marxianus, and lactic acid bacteria is bacillus acidophilus and Lactobacillus fermenti, and acetic acid bacteria is Acetobacter pasteurianus, and vaccination ways is to be 1 × 10 by the inoculum concentration inoculating active bacterium number of 75g/t9The active microbe powder of cfu/g.
The fermentation temperature of described step 3 and step 4 is 28～32 DEG C.
The aperture of the filter membrane that described membrane filtration is degerming is 0.1～0.2 μm.
In described step 4, it is simultaneously introduced the powder of Radix Puerariae accounting for fermentation liquid 5wt% and the agar powder accounting for fermentation liquid 0.1wt% with dry Ganoderma powder.
Any of the above-described kind has the application in preparation has the active product of suppression human lung carcinoma cell line A549, human cervical carcinoma cell lines Hela and HepG2 cell lines growth of the comprehensive enzyme liquid of anti-tumor activity.
In the present invention, Ganoderma is the sporophore of On Polyporaceae Ganoderma, and sweet-bitter flavor, property are flat, can tranquilizing by nourishing the heart, quiet lung QI invigorating, regulating the flow of vital energy silt, liver nourishing spleen invigorating.Through a large amount of clinical researches, the Ganoderma curative effect to degree of having nothing in common with each other such as neurasthenia, hyperlipemia, angina pectoris, arrhythmia, Keshan disease, plateau discomfort disease, hepatitis, hemorrhagic fever, dyspepsia, tracheitis, tumors.
Rhizoma Dioscoreae is Dioscoreaceae plant Rhizoma Dioscoreae dry rhizome, and sweet in the mouth, property are flat, has and nourishes strong, aid digestion, to hold back sweating due to debility, antidiarrheal effect.There is blood pressure lowering, slow down aging, antitumor and treatment dermatosis effect.
Rhizoma Zingiberis Recens have in Chinese medicine and pharmacy dissipate, effect of preventing or arresting vomiting cough-relieving, be treatment nicotinic acid-alcohol repellency ulcer active drug, zingiberene have protection gastric mucosal cell effect；Meanwhile, Rhizoma Zingiberis Recens has antibacterial, antioxidation；Rhizoma Zingiberis Recens juice can grow by anticancer to a certain extent, adds Rhizoma Zingiberis Recens extract and can alleviate the side effect of tumour medicine in some antitumor drug.
Fructus Lycopersici esculenti is nutritious, has the title in " vitamin warehouse ".It contains protein, fat, saccharide, several mineral materials, trace element.Wherein the content of vitamin C and Citrin is the highest.Have slow down mottle slow down aging, anticancer, can blood fat-reducing blood pressure-decreasing, the generation of anti-tampon and digestion promoting effect.
Fructus Fici can invigorating the stomach and promoting bowel movement, subduing swelling and detoxicating.Can be used for the diseases such as inappetence, abdominal distention, constipation due to hemorrhoids, dyspepsia, diarrhoea, hypogalactia, laryngopharynx swelling and pain, hematodiarrhoea, cough ant phlegm.Dyspepsia person, inappetence person, hyperlipemic patients, hyperpietic, patients with coronary heart disease, arteriosclerotic, cancer patient, constipation person suitably eat.
Fructus Mali pumilae is referred to as " general practitioner ", its nature and flavor are gentle, containing abundant carbohydrate, vitamin and trace element, there are saccharide, organic acid, pectin, protein, calcium, phosphorus, potassium, ferrum, vitamin A, vitamin B, vitamin C and dietary fiber, another containing malic acid, tartaric acid, carotene, it is that all vegetables and fruits Middle nutrition are worth closest to perfect one.There is reduction cholesterol, cancer-resisting, cleaning blood vessel, promote the effect such as gastrointestinal peristalsis, maintenance acid-base balance, fat-reducing, antidiarrheal.
The sarcocarp of pears contains the organic acid such as abundant pulp, glucose and malic acid, additionally contains the multivitamins such as protein, fat, calcium, phosphorus, ferrum and carotene, vitamin B1, vitamin B2, nicotinic acid, ascorbic acid.There is blood pressure lowering, clearing away lung-heat to relieve cough, appetizing hepatoprotective, cancer-resisting, the effect of diuresis relieving constipation.
Comprehensive enzyme liquid prepared by the present invention is with fresh fruit of vegetables etc. as raw material, sweat is gentle, whole process is biological metabolism effect, the nutrient substance that food materials are intrinsic can be retained to greatest extent, simultaneously, the effect of undergoing microbial fermentation creates again multiple new metabolite, had both met modern's diversity, comprehensive nutritional need, and had been prone to again digest and assimilate.
The preparation method of a kind of comprehensive enzyme liquid with anti-tumor activity of the present invention, operating procedure is simple, raw material sources are extensive, and various vegetables, fruit and mushroom carry out matched combined, nutritious comprehensively, each component is through rational allocation, ferment excellent taste, is suitable for daily drunk, invisible in can suppressing human body growth of tumour cell, enhancing immunity, promotes healthy.
Detailed description of the invention
Embodiment one
The preparation method of a kind of comprehensive enzyme liquid with anti-tumor activity of the present invention, concrete operation step is as follows:
(1) selecting fresh and ripe fruit and vegerable is fermentation raw material, rejects pest and disease damage fruit and mildew and rot vegetables and fruits, cleans and drain surface moisture, weighing.Take pears 3600g, Fructus Mali pumilae 3600g, Fructus Fici 2400g, Rhizoma Zingiberis Recens 600g, Rhizoma Dioscoreae 2400g and Fructus Lycopersici esculenti 3400g, smash with beater；Select first-class dry Ganoderma 4000g, pulverize standby；
(2) stirring after fruit and vegetable slurry mixing above-mentioned steps (1) processed, the cellulase and the 15g pectase that add 15g carry out enzymolysis, and hydrolysis pH scope is 4.0～5.5, and temperature range is 40～50 DEG C, enzymolysis 5～10 hours；Being added followed by the α-amylase of the lipase of 3g, the protease of 3g and 16g and carry out enzymolysis, hydrolysis pH scope is 6.0～7.5, and temperature range is 35～45 DEG C, carries out enzymolysis 3～7 hours；
(3) being placed in sealed fermenting tank by the enzymolysis solution that above-mentioned steps (2) processed, add the sucrose of 4000g, add yeast Kluyveromyces marxianus, bacillus acidophilus and Lactobacillus fermenti, it is 1 × 10 that three of the above bacterium is active bacteria number9The active microbe powder of cfu/g, inoculum concentration is every kind of 1.5g, and 28～32 DEG C ferment 60 days；
(4) carry out the fruit and vegerable fermentation liquid that above-mentioned steps (3) obtains settling, filter and remove residue；
(5) adding Ganoderma powder in the fermentation liquid that above-mentioned steps (4) obtains, 28～32 DEG C carry out ferment in second time 60 days；
(6) adding 1.8g active bacteria number in the fermentation system of above-mentioned steps (5) is 1 × 109The Acetobacter pasteurianus of cfu/g, 28～32 DEG C ferment 40～60 days；
(7) fermentation liquid that above-mentioned steps (6) obtains is carried out membrane filtration degerming；
(8) fermentation liquid that above-mentioned steps (7) obtains is carried out 75 DEG C and maintains sterilization in 20 minutes, then sterile filling, be packaged into enzyme liquid finished product.
Embodiment two
The preparation method of a kind of comprehensive enzyme liquid with anti-tumor activity of the present invention, concrete operation step is as follows:
(1) selecting fresh and ripe fruit and vegerable is fermentation raw material, rejects pest and disease damage fruit and mildew and rot vegetables and fruits, cleans and drain surface moisture, weighing.Take pears 3600g, Fructus Mali pumilae 3600g, Fructus Fici 2400g, Rhizoma Zingiberis Recens 600g, Rhizoma Dioscoreae 2400g and Fructus Lycopersici esculenti 3400g, smash with beater；Select first-class dry Ganoderma 4000g, pulverize standby；
(2) stirring after fruit and vegetable slurry mixing above-mentioned steps (1) processed, the cellulase and the 15g pectase that add 15g carry out enzymolysis, and hydrolysis pH scope is 4.0～5.5, and temperature range is 40～50 DEG C, enzymolysis 5～10 hours；Being added followed by the α-amylase of the lipase of 3g, the protease of 3g and 16g and carry out enzymolysis, hydrolysis pH scope is 6.0～7.5, and temperature range is 35～45 DEG C, carries out enzymolysis 3～7 hours；
(3) being placed in sealed fermenting tank by the enzymolysis solution that above-mentioned steps (2) processed, add the sucrose of 4000g, add yeast Kluyveromyces marxianus, bacillus acidophilus and Lactobacillus fermenti, it is 1 × 10 that three of the above bacterium is active bacteria number9The active microbe powder of cfu/g, inoculum concentration is every kind of 1.5g, and 28～32 DEG C ferment 60 days；
(4) carry out the fruit and vegerable fermentation liquid that above-mentioned steps (3) obtains settling, filter and remove residue；
(5) adding dry Ganoderma powder in the fermentation liquid that above-mentioned steps (4) obtains, be simultaneously introduced the powder of Radix Puerariae accounting for fermentation liquid 5wt% and the agar powder accounting for fermentation liquid 0.1wt%, mixing, 28～32 DEG C carry out ferment in second time 60 days；
(6) adding 1.8g active bacteria number in the fermentation system of above-mentioned steps (5) is 1 × 109The Acetobacter pasteurianus of cfu/g, 28～32 DEG C ferment 40～60 days；
(7) fermentation liquid that above-mentioned steps (6) obtains is carried out membrane filtration degerming；
(8) fermentation liquid that above-mentioned steps (7) obtains is carried out 75 DEG C and maintains sterilization in 20 minutes, then sterile filling, be packaged into enzyme liquid finished product.
The anti tumor activity in vitro of enzyme liquid, indicator cells strain behaviour lung cancer cell types, human cervical carcinoma cell lines Hela and HepG2 cell lines used is surveyed with mtt assay.Concrete operation step is as follows:
(1) peptic cell and cell counting
After cell dissociation is made 1mL cell suspension, take 100 μ L in clean cell dish, add 8mL culture fluid, carry out cell counting with blood counting chamber, until the concentration of cell is about 6 × 104Individual/mL.
(2) plating cells
Layout: in order to prevent edge effect, at a certain amount of sterilized water of addition that made a circle in 96 orifice plate weeks.Each sample uses stock solution, two Concentraton gradient of twice diluent, and three parallel is measured；Negative, positive, blank respectively do three parallel.
Sample well and feminine gender, Positive control wells add 80 μ L cell suspension, and blank control wells adds 80 μ L culture fluid.After being joined by cell suspension in 96 orifice plates, whether inverted microscope observation of cell is evenly distributed, 37 DEG C, 1.5%CO2Cultivate in incubator.
(3) testing sample is added
After cell cultivates 24h, add testing sample.Take 20 μ L sample to add in hand-hole.
Negative control: take 20 μ L sterilized water and add in hand-hole.
Positive control: 10 μ L concentration are that the cisplatin solution of 10 μ g/mL adds 10 μ L culture fluid.
Blank: 20 μ L fresh mediums.
(4) MTT solution is added
After cultivating 72h in 37 DEG C of incubators, taking out culture fluid, every hole adds 100 μ LPBS buffer, adds 5mg/mLMTT solution, every hole 10 μ L.
(5) MTT stop buffer is added
After adding MTT solution reaction about 3～4h, add MTT stop buffer, every hole 100 μ L.
(6) OD value is surveyed
After adding MTT stop buffer 9～12h, microplate reader is used to measure (wavelength 595nm) OD value.Calculate the sample suppression ratio to tumor cell.
Suppression ratio computational methods are as follows:
Suppression ratio=(negative control OD value-experimental group OD value)/(negative control OD value-blank group OD value) * 100%
Through measuring, the anti-tumor activity result of prepared comprehensive enzyme liquid is as shown in table 1:
The table 1 comprehensive enzyme liquid extracorporeal extracorporeal suppression to three kinds of tumor cells
| Dilution factor | A549 | Hela | HepG2 |
| Embodiment one stock solution | 60.6% | 63.4% | 59.0% |
| One 2 times of diluents of embodiment | 51.6% | 55.2% | 47.1% |
| Embodiment two stock solution | 68.4% | 72.6% | 61.0% |
| 22 times of diluents of embodiment | 63.4% | 56.7% | 52.5% |
From the above, comprehensive enzyme liquid prepared by the present invention is balanced in nutrition comprehensively, taste is good, has certain anti-tumor activity simultaneously, daily drunk, can enhancing immunity, building body.
And, by upper table it can also be seen that after adding in fermentation liquid and accounting for the powder of Radix Puerariae of fermentation liquid 5wt% and account for the agar powder of fermentation liquid 0.1wt%, the comprehensive enzyme liquid of the present invention has more excellent anti-tumor activity.
Comprehensive enzyme liquid prepared by the present invention capable of direct drinking or dilution after drink.
Above are only the detailed description of the invention of the present invention, but the design concept of the present invention is not limited thereto, all changes utilizing this design that the present invention carries out unsubstantiality, the behavior invading scope all should be belonged to.
Claims (9)
1. the preparation method of a comprehensive enzyme liquid with anti-tumor activity, it is characterised in that comprise the steps:
Step one, cleans pears, Fructus Mali pumilae, Fructus Fici, Rhizoma Zingiberis Recens, Rhizoma Dioscoreae and Fructus Lycopersici esculenti, smash, mixes；
Step 2, adding the cellulase of 0.05～0.1wt% in the slurry of mixing and the pectase of 0.05～0.1wt% carries out enzymolysis, the α-amylase of the protease and 0.1wt% that are added followed by lipase, 0.01～the 0.02wt% of 0.01～0.02wt% carries out enzymolysis；
Step 3, is placed in enzymolysis solution in sealed fermenting tank, adds the sucrose of 25wt%, adds yeast and lactic acid bacteria, mix homogeneously, ferment 60 days, by fermentation liquid sedimentation, filter and remove residue；
Step 4, adds dry Ganoderma powder in fermentation liquid, carries out ferment in second time, ferment 60 days after mixing；Add acetic acid bacteria, anaerobic fermentation 40～60 days afterwards；
Step 5, the fermentation liquid obtained is carried out membrane filtration degerming, 75 DEG C maintain 20 minutes sterilization, sterile filling.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: the parts by weight of described pears, Fructus Mali pumilae, Fructus Fici, Rhizoma Zingiberis Recens, Rhizoma Dioscoreae, Fructus Lycopersici esculenti, dry Ganoderma powder and sucrose are: pears 16～20 parts, Fructus Mali pumilae 16～20 parts, Fructus Fici 10～14 parts, Rhizoma Zingiberis Recens 2～4 parts, Rhizoma Dioscoreae 10～14 parts, Fructus Lycopersici esculenti 16～18 parts, dry Ganoderma powder 18～22 parts, sucrose 20～30 parts.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: in described step 2, the hydrolysis pH scope of cellulase and pectase is 4.0～5.5, and temperature range is 40～50 DEG C, enzymolysis 5～10 hours；The hydrolysis pH scope of lipase, protease and α-amylase is 6.0～7.5, and temperature range is 35～45 DEG C, enzymolysis 3～7 hours.
A kind of preparation method of the comprehensive enzyme liquid with tumor promotion, it is characterized in that: described yeast is yeast Kluyveromyces marxianus, lactic acid bacteria is bacillus acidophilus and Lactobacillus fermenti, and vaccination ways is to be 1 × 10 by the inoculum concentration inoculating active bacterium number of 75g/t9The active microbe powder of cfu/g.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: described acetic acid bacteria is Acetobacter pasteurianus, and vaccination ways is to be 1 × 10 by the inoculum concentration inoculating active bacterium number of 75g/t9The active microbe powder of cfu/g.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: the fermentation temperature of described step 3 and step 4 is 28～32 DEG C.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: the aperture of the filter membrane that described membrane filtration is degerming is 0.1～0.2 μm.
A kind of preparation method of the comprehensive enzyme liquid with anti-tumor activity, it is characterised in that: in described step 4, it is simultaneously introduced the powder of Radix Puerariae accounting for fermentation liquid 5wt% and the agar powder accounting for fermentation liquid 0.1wt% with dry Ganoderma powder.
9. described in claim 1 to 8, any one has the application in preparation has the active product of suppression human lung carcinoma cell line A549, human cervical carcinoma cell lines Hela and HepG2 cell lines growth of the comprehensive enzyme liquid of anti-tumor activity.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN102940288A (en) * | 2012-11-19 | 2013-02-27 | 商丘市饮之健食品有限公司 | Composite enzyme beverage and preparation process method thereof |
| CN104522820A (en) * | 2015-01-19 | 2015-04-22 | 中国食品发酵工业研究院 | Mixed fruit and vegetable fermented product and preparation method thereof |
| CN104664270A (en) * | 2014-07-09 | 2015-06-03 | 普正药业股份有限公司 | Fresh and alive traditional Chinese medicine fruit-vegetable enzyme and preparation method thereof |
| CN104886572A (en) * | 2015-06-02 | 2015-09-09 | 广州加海生物科技有限公司 | A fruit and vegetable ferment beverage and a preparation method thereof |
| CN105166933A (en) * | 2015-10-28 | 2015-12-23 | 青海尤尼克生物技术有限公司 | Composition containing Chinese wolfberry fruit extract and fruit ferment and preparation method thereof |
-
2016
- 2016-04-11 CN CN201610221798.1A patent/CN105815641A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN102940288A (en) * | 2012-11-19 | 2013-02-27 | 商丘市饮之健食品有限公司 | Composite enzyme beverage and preparation process method thereof |
| CN104664270A (en) * | 2014-07-09 | 2015-06-03 | 普正药业股份有限公司 | Fresh and alive traditional Chinese medicine fruit-vegetable enzyme and preparation method thereof |
| CN104522820A (en) * | 2015-01-19 | 2015-04-22 | 中国食品发酵工业研究院 | Mixed fruit and vegetable fermented product and preparation method thereof |
| CN104886572A (en) * | 2015-06-02 | 2015-09-09 | 广州加海生物科技有限公司 | A fruit and vegetable ferment beverage and a preparation method thereof |
| CN105166933A (en) * | 2015-10-28 | 2015-12-23 | 青海尤尼克生物技术有限公司 | Composition containing Chinese wolfberry fruit extract and fruit ferment and preparation method thereof |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244372A (en) * | 2016-08-05 | 2016-12-21 | 贵州省中国科学院天然产物化学重点实验室 | A kind of ferment Rhizoma Gastrodiae health-preserving beverage improving sleep and preparation technology and application |
| CN106244372B (en) * | 2016-08-05 | 2020-06-02 | 贵州省中国科学院天然产物化学重点实验室 | Enzyme-fermented gastrodia elata health-preserving beverage for improving sleep and preparation process and application thereof |
| CN106635916B (en) * | 2017-01-04 | 2020-05-15 | 厦门元之道生物科技有限公司 | Acetobacter orientalis YZD-09 and application thereof |
| CN106635916A (en) * | 2017-01-04 | 2017-05-10 | 厦门元之道生物科技有限公司 | Acetobacter orientalis YZD-09 and application thereof |
| CN107114635A (en) * | 2017-03-13 | 2017-09-01 | 辛庆忠 | A kind of enzyme liquid and preparation method thereof |
| CN107019213A (en) * | 2017-04-21 | 2017-08-08 | 湖南双晟科技信息咨询有限公司 | A kind of water chestnut ferment and preparation method thereof |
| CN107041529A (en) * | 2017-05-26 | 2017-08-15 | 江门市昊能生物科技有限公司 | A kind of Xinhui citrus reticulata fruit lactic acid ferment juice and preparation method thereof |
| CN107183697A (en) * | 2017-07-22 | 2017-09-22 | 厦门元之道生物科技有限公司 | A kind of agaric sea-tangle enzyme liquid with effect for reducing blood fat and preparation method thereof |
| CN107495038A (en) * | 2017-08-26 | 2017-12-22 | 安徽阜南常晖食品有限公司 | A kind of preparation method of high-quality fermentation tomato juice |
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| CN112957430A (en) * | 2021-02-06 | 2021-06-15 | 吉林大学 | Application of biological intensified cell nutrient in preparing anti-liver cancer medicine |
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Application publication date: 20160803 |