CN106579387A - Arabinose-enzyme composition and preparation method thereof - Google Patents
Arabinose-enzyme composition and preparation method thereof Download PDFInfo
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- CN106579387A CN106579387A CN201611167731.0A CN201611167731A CN106579387A CN 106579387 A CN106579387 A CN 106579387A CN 201611167731 A CN201611167731 A CN 201611167731A CN 106579387 A CN106579387 A CN 106579387A
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- arabinose
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
An arabinose-enzyme composition is prepared from an enzyme that is prepared from vegetable or/and fruit material, wherein by weight L-arabinose accounts for 3-25 parts, xylitol accounts for 3-15 parts, and the enzyme accounts for 10-200 parts. By mulberry fruit of 20% and above, the enzyme is prepared, and 3-18 parts of oligosaccharide is added. In the arabinose-enzyme composition, the mass ratio of L-arabinose, xylitol and oligosaccharide is (5-10):(5-10):(8-15), and 1-5 parts of edible selenium powder is added.
Description
Technical field
The present invention relates to a kind of health food, more particularly to a kind of arabinose enzyme composition and preparation.
Background technology
CN105285383 proposes a kind of Arabic syrup and preparation method thereof, and syrup includes water and saccharic composition, water and sugar
Constituent mass volume ratio is 1~1.4:1;Saccharic composition is included by mass fraction:7~13 parts of L-arabinose, 5~9 parts of wood
Sugar and 2~5 parts of oligomeric xylose.Saccharic composition is included by mass fraction:10~12 parts of L-arabinose, 6~8 parts of xylose and
2.5~3.5 parts of oligomeric xylose.But the function of L-arabinose, xylose, oligomeric xylose, than relatively similar, three kinds of functional sugars are mutual
Effect is strong, especially the latter two functionally complete class it is same.It is beneficial to the quantity of flora in human body intestinal canal;Blood glucose can be controlled
Raise, suppress lipogenesis so that arabinose Fructus Mori compositionss are in sides such as prevention diabetes, constipation, three-hypers and eliminating toxin and beautifying the skin
Face has good effect, and long-term taking can allow intestinal beneficial flora to be bred at double.
The L-arabinose of arabinose especially main effect has been approved for new resources also known as Arabinose, pectose
Food.Although the half of L-arabinose sugariness only sucrose, the saccharase of sucrose is digested in energy selectivity negatively influencing small intestinal,
Suppress the absorption of sucrose so as to have obvious effect even directly to have a medical function at the control aspect such as blood glucose, the day of single 15g
Dosing is even used to treat the type-II diabetes at initial stage.Oligosaccharide is except the physicochemical properties with general utility functions oligosaccharide
Outward, most noticeable physiological property is that it can be obviously improved microbial population ratio in intestinal, and it is enteral bacillus bifiduss
The activation and proliferation factor, for probiotic bacteria carbon source is provided, and is a class non-digestible oligosaccharide, not by gastric acid and digestion enzymatic degradation, directly
Probiotic bacteria is preferably into big enteral to be utilized, while producing various organic acid, reduces gut pH, suppress harmful bacteria growing,
Probiotic bacteria is set to breed in a large number in intestinal.The generation of enteral corrupt substance can be reduced and be suppressed, suppresses the growth of noxious bacteria, be adjusted
Intestinal inner equilibrium;Trace elements iron, the absorption of calcium can be promoted and utilized, to prevent osteoporosis;Hepatotoxin, energy can be reduced
The organic acid of anticancer is generated in intestinal, there is significant preventing cancer function;And pure taste is fragrant and sweet good to eat, with the adipose fragrance of class
With tasty and refreshing soapy feeling.Typically have:Fructus Hordei Germinatus oligose glucose, fructose oligosaccharides, soybean oligo saccharide, polydextrose, gala
Sugared oligosaccharide, oligofructose, oligomeric xylose (β-Isosorbide-5-Nitrae glycosidic bond combination).
Xylitol is a kind of sweeting agent with nutritive value, and sugariness is suitable with sucrose, is sucrose and Fructus Vitis viniferae sugar substitute.
It is its a big feature that heat is low:2.4 calories per gram, than other little carbohydrates 40%.Welcome by diabetes patient very much.
It is the intermediate of human body carbohydate metabolism, and insulin is lacked in vivo to be affected in the case of carbohydrate metabolism, to promote without the need for insulin, xylose
Alcohol can also pass through cell membrane, be absorbed by tissue utilization, for cell with nutrition and energy, and blood glucose value will not be caused to raise, and eliminate
Diabetes patient's symptom more than three (polyphagia, polydipsia, polyuria), is the sugar substitutes of the trophism for being best suitable for patients with diabetes mellitus,
Sweeting agent, supplementary and the auxiliary therapeutical agent that diabetes patient can be is used.Xylitol can also improve liver function.It can promote
Liver glycogen synthesizes, and blood glucose will not rise, and having to hepatopath improves liver function and lipotropic effect.Xylitol is also preventing decayed tooth
The best sweeting agent of tooth, it is not produced cariogenic bacterial fermentation in oral cavity and is utilized, and suppresses the generation of streptococcus growth and acid;Energy
Promote salivation, slow down pH value decline, reduce the acid etching of tooth, prevent dental caries and reduce the generation of dental plaque, can consolidate
Tooth.Xylitol can reduce dental caries this characteristics, in high-risk rate crowd (dental caries incidence rate height, Undernutrition, oral hygiene
Level is low) and low risk crowd (being taken care of one's teeth using current all of cleaning of teeth measure, tooth cavity generation rate is low) in be
It is suitable for.Xylitol also has weight losing function.Energy is provided for human body, synthesizes glycogen, the protein in reduction fat and hepatic tissue
Consume, liver is protected and is repaired, eliminate the generation that ketoboidies is harmful in human body, will not be worry of getting fat because edible.Wood
Sugar alcohol biological stability is good, good hygroscopicity.
As the mankind are to health care and the requirement of auxiliary treatment diabetes, need to prepare one kind with health care, convenient food
Arabinose compositionss.
Ferment refers to the biological effect active substance that bacterium is participated in from fermentation.Bacterium source ferment favourable to human body in prebiotic ferment
Element includes:What active probiotic (probiotics) body, probiotic bacteria were produced is respectively enzyme (enzyme), prebiotic granulose
(Lactobacilluspolysaccharide), lactein (Lactobacteriosin) four is most of.Active probiotic sheet
Body, including lactic acid bacteria and Bei La yeast, are that national governments and linked groups of the United Nations allow the safe bacterial strain present in food.
Have been widely used for various therapies.Generally comprise in prebiotic ferment:Lactobacillus acidophilus, Fructus rhamni (Rhamnus davurica Pall.) saccharobacillus, the bright coccus of a beading,
Bifidobacterium adolescentiss, Lactobacillus plantarum, Bifidobacterium bifidum, Lu Shi lactic acid bacterias, being even more in reinforcing ferment includes 150 kinds of high activities
Probiotic bacteria.The enzyme that probiotic bacteria is produced in raw ferment is exactly in fact Louis. the ferment (ferment) of Pasteur original definition.Including:
Sour enzyme system is produced, is synthesized polysaccharide enzyme system, the enzyme system of decomposing nitrosamine, is reduced cholesterol enzyme system, control endotoxin enzyme system, reduces fat
Enzyme system, synthetic vitamin enzyme system, decomposition cholic acid enzyme system, oxalic acid deshydroxy enzyme, superoxide dismutase.Prebiotic granulose in ferment
(Lactobacillus polysaccharide), lactein (Lactobacteriosin) also serve as safe effect thing
Matter, is being widely used in the fields such as treatment of cancer, food antiseptic.All prebiotic ferment all contain bacterium source ferment, therefore they
Constitute the general utility functionss of prebiotic ferment.
The plant source ferment contained in prebiotic ferment, including:Enzyme (enzyme), phytochemistry element (Phytochemicals) two
Big class.Enzyme in the ferment of plant source includes:Superoxide dismutase, Fructus Ananadis comosi protease, papain, Vc digestive enzyme, Fructus Fici disappear
Change phytochemistry element point primary metabolite and secondary that enzyme, chitinase, oxalic acid deoxygenase, myrosin ... are planted in the ferment of source
The class of metabolite two.According to chemical composition then include:Polyphenol, Saponin, polysaccharide, terpene, ketone, alkene, glycosides, phytosterol, phytohormone etc..In
Magnificent national rarity, the traditional Chinese medical science and dietetic therapy, its core content is exactly rationally to be got rid of illness health preserving using these phytochemistry elements.
The content of the invention
It is an object of the present invention to provide a kind of arabinose enzyme composition and preparation method.Compositionss optimize intestinal with
Ecology, be provided in particular in the fermented product such as Fructus Mori based on L-arabinose, xylitol and oligosaccharide in intestinal with microbial bacteria
The reciprocal effect fallen with cyton, therefore, it is possible to have the healthy effect for more preferably adjusting human body.
The technical scheme is that, arabinose enzyme composition, including ferment, ferment is that vegetable or fruit are former
Ferment prepared by material, quality formula is as follows:The L-arabinose of 3-25 parts, 3-15 part xylitol, ferment 10-200 parts.
With the ferment made above of Fructus Mori mass content 20%, and add 3-18 part oligosaccharide.
Semisolid can be prepared in compositionss, it is also possible to add water, the quality of water is the 0.2-3 of all substances quality
Times.
L-arabinose of the purity that arabinose is extracted using biofermentation up to more than 99.0%;L-arabinose:Wood
Sugar alcohol component mass ratio is 1-1.4:1-4;Oligosaccharide includes:Fructose oligosaccharides, polydextrose, GOS, oligomeric wood
Sugar, oligomeric isomaltose, oligomeric lactose, soybean oligo saccharide.
Ferment 30-60 parts, and add edible fiber 10-80 parts.Edible fiber selects Herba bromi japonici (skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, sea
The algae such as band, Eucheuma muricatum (Gmel.) Web.Van Bos., equator red algae Eucheuma muricatum (Gmel.) Web.Van Bos., including (Equations of The Second Kind) water soluble dietary fiber:Low fat pectin, high fat pectin,
Apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin, agar powder, carboxymethyl cellulose water soluble dietary fiber.
The quality parts ratio of L-arabinose, xylitol and oligomeric xylose is 5-10 in arabinose compositionss:5-10:8-
15。
Described arabinose enzyme composition, then add edible selenium powder, addition quality is 1-5 parts.
Ferment raw material using 20-50% quality Fructus Mori, and using Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactobacillus and
Shellfish draws yeast addition 1-2%, 25-32 DEG C of 7-25 days Production Time.
Ferment adds fresh Fructus Chaenomeliss and/or white turnip, or even the blue certain kind of berries.
The preparation method of described arabinose compositionss, adds xylitol, L-arabinose and oligosaccharide in water, heat up
To 80 DEG C of pasteurizing temperatures, add after cooling and 30-45min is incubated under ferment, stirring condition, that is, arabinose compositionss are obtained
Bottling.Addition 5-18 part oligosaccharide.Another addition water, the quality of water is 0.2-10 times of above-mentioned all substances quality.
Product of the purity that arabinose is extracted using biofermentation up to more than 99.0%.L-arabinose:Xylitol group
Mass ratio is divided to be 1~1.4:1~4;
Ferment in particular by Fructus Mori 20-100% quality, other adopt Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactic acid bacteria drink
Sour bacterium and Bei La yeast addition 1-2%, 25-32 DEG C of 7-25 days Production Time.Raw material can also add fresh Fructus Chaenomeliss, white turnip in ferment.
The preparation method of arabinose compositionss, ferment is prepared and completed;Xylitol, L-arabinose and oligomeric are added in water
Sugar, edible fiber, are warming up to 80 DEG C of pasteurizing temperatures, add after cooling and 30-45min is incubated under ferment, stirring condition, that is, make
Obtain the bottling of arabinose compositionss.
White turnip ferment can eliminate bodily waste rich in the amylase for decomposing harmful intestinal tract material, adjust intestinal group rings
Border, keeps enteral various bacteria balance.The ferment that Fructus Lycopersici esculenti produces gives protection against cancer, antitumaous effect is more higher than lycopene.It is green in green tea
Tea phenol is a kind of glutathione transferase, can increase the amount that Fructus Lycopersici esculenti produces ferment.Fructus Lycopersici esculenti ferment is to carcinoma of prostate and some digestion
The preventive effect of road cancer it is further preferred that.7-25 days or longer Production Time.
Active lactobacillus and Bei La yeast, especially shellfish draw yeast production yeast selenium, add in yeast culture medium suitable
The nothing of amount typically eats selenium powder yeast selenium, and organic selenium is produced to the accumulation ability of selenium using yeast, yeast is then peeled off, by nothing
Breaking cellular wall is produced after machine selenium is removed.The existence form of selenium is more complicated in the constituent yeast selenium of yeast selenium, including seleno egg ammonia
Acid, seleno Guang aminoacid, CH3SeH, selenoprotein and other selenides.Selenomethionine is also a kind of single selenium source.System
After the completion of standby day oral dose deal addition L-arabinose.
Take:Every morning is drunk after 200 milliliters of warm waters on an empty stomach, drinks the ferment combination of 20-50 milliliters (or more) Arab
Thing.The symptom for occurring similar " borborygmus/diarrhoea " during taking is that the intestines and stomach normally reacts.Suitable population:Malaise, Jing often feel
Emit, drowsy, stomach QI-insufficiency, constipation, it is fat, like drinking, long-term smoking, depression person, three-hypers, have cancer family history etc..
Fermentable ferment is that, with raw materials such as one or more vegetable or fruit, what Jing multiple beneficials bacterium was fermented and produced gives birth to rich in dimension
The functional microbial fermented food of the nutritional labelings such as element, enzyme, mineral and secondary metabolite.Enzyme microb have delay
The plurality of health care functions such as aging, anti-inflammation, purification blood, enhancing human body immunity ability and detoxicating and fighting cancer.Fermentation is mainly profit
With polysaccharide in the presence of yeast, ethanol, carbon dioxide, water and aromatic substance are decomposed into, so as to prepared flavour is sour-sweet, mouthfeel
The mulberry enzyme of sweet-smelling.The antioxygenic property of ferment is fermented essentially from sweat, Fructus Mori in the presence of microorganism,
Organic acid concentration increase in fermentation liquid makes the pH value of fermentation liquid 4 or so, and sour environment causes protein to be hydrolyzed to aminoacid.With
This simultaneously, yeast will vegetable fruit expect in enrich and complicated macromole aldehydes matter change into small molecule, total phenol content liter
Height, aldehydes matter is easy to provide a hydrion and stablize by resonance hybrid, makes mulberry enzyme DPPH free radical scavenging
Ability is significantly improved.In addition, the polyhydroxy on the A rings and B rings of chromocor compound molecule replaces and the 3- hydroxyls of free radical replace
Base can strengthen the Scavenging activity of the superoxide radical of ferment.Meanwhile, mulberry enzyme antioxidant content during the fermentation increases
Plus, its reducing power is significantly enhanced.
Enzyme microb is that fermentation of the active probiotic body to vegetable and fruit is obtained, and active probiotic mainly includes lactic acid
Bacterium and Bei La yeast, are that national governments and linked groups of the United Nations allow the safe bacterial strain present in food.Extensively used
In various therapies.Also include in prebiotic ferment:Lactobacillus acidophilus, Fructus rhamni (Rhamnus davurica Pall.) saccharobacillus, the bright coccus of a beading, youth bifid bar
Bacterium, Lactobacillus plantarum, Bifidobacterium bifidum, Lu Shi lactic acid bacterias, being even more in reinforcing ferment includes that more than 100 kinds of high activity is prebiotic
Bacterium colony.Ferment not only make use of the plant source ferment contained in veterinary antibiotics, and carry out attenuation synergistic to edible composition.Cause
This has very magical biological effect.Specifically this kind of plant source ferment has:Sulforaphane, resveratrol, ginsenoside, spirit
Sesame polysaccharide, anthocyanidin, limonin, bitter melon polysaccharide, gingerol, isoflavone, hydroxymalonic acid.
The preparation method of the composite plant ferment containing profitable probliotics, has selected the various fruits vegetable, medicine food dual purpose plant to be
Fermentation substrate, and using various probiotic bacteria/yeast as fermentation inoculum, using multicycle discontinuous fed-batch fermentation, the multistage depths of Jing
After layer fermentation, the composite plant ferment containing profitable probliotics is prepared.The species of the enzyme that prepared composite plant ferment contains
Many, activity is high, and good stability is affected by environment little.Using this method different time sections using the different substrates of different strain fermentation,
Make the different extracts active ingredients contained by all kinds of fermentation substrates more complete, whole system fermentation is more fully coordinated.Ferment
Element constitutes total performance of human life phenomenon in catalysis in vivo thousands of kinds of biochemical reactions.Machine can comprehensively be controlled and optimized to ferment
Internal milieu so as to keep dynamic equilibrium, that is, the equilibrium between yin and yang state described in the traditional Chinese medical science, is for healthy state.Ferment is in room temperature
In oxidation reaction produced by energy, and the storage and utilization of the energy, showing as ferment can enhance metabolism, and adjust
Section secretion, improves digestive function, and metabolic waste and toxin are eliminated in time, promotes the raw bacteria growing that overflows in intestinal, and keeps intestinal
The good intestinal environment of bacterial micro-ecological balance.Ferment carries out incessantly from many aspects internal efforts at environmental protection, makes human body dirty
Internal organs function keeps optimum state, improves body constitution, disease-prevention health health.Ferment can improve and maintain the normal bacterium phase of intestinal, have bowel relieving to arrange
Toxic action, it is possible to reduce enteral noxious substance is entered in blood, to keep blood pure.
Beneficial effect:Due to the absorption to sugar of the intestinal that has its source in of hyperglycemia, undue absorption glucose enters blood
Liquid causes insulin resistance or opposing, modal insulin resistance type and a kind of condition of illness contact for being referred to as metabolism syndrome
Together.Insulin resistance can develop into thoroughly type-II diabetes.Commonly post prandial hyperglycemia, in such case
Under, pancreas beta cell cannot produce enough insulins to keep normal glycemic levels.Beta cell nothing in the case of hyperglycemia
It is the feature changed from insulin resistance to type-II diabetes that power secretes more insulins.Emphasis is aimed at this by the present invention,
Optimization intestinal and ecology, comprehensive mechanism is more complicated, it should which Fructus Mori based on L-arabinose and oligosaccharide etc. are in intestinal
With microbe colony and the reciprocal effect of cyton, somatic cell is excited in beneficial agents, stimulates beneficial to produce, Mulberry on surface
Shen etc. and oligosaccharide, fiber can promote the wriggling of intestinal, and more deep effect is nutrition and strong intestinal cell, is had in absorption
Beneficial excitor substance (enzyme, peptide and small protein including producing), therefore, it is possible to adjust the immunity of human body, although on surface
Defecation is significantly improved, but the malicious and net blood of Eradicates more has influence on the health of human liver, so as to also remote-effects arrive kidney etc..Especially
Intestinal hinders absorption of the intestinal to multitudinous sugar because of L-arabinose, and the nutrition that glycogen becomes probiotic bacteria again is former, more have stimulated probiotic bacteria
Secretion beneficial products.
For from the origin of life, intestinal is the foundation of acquired constitution, and the intestinal of health is to absorb transporting food and various nutrition
The nutrition of product, the intestinal of the health immune organ that to be again function maximum with volume, the present invention based on the nutrition of intestinal, nourishing and
Health care can fully improve the transporting ability of intestinal.
Point out when dietary fiber is spoken of in health:" ... dietary fiber plays in vivo critical function, act as health
The role of bodyguard.Dietary fiber has a stimulation intestinal peristalsis promoting, increases the volume of intestinal contentses, reduce that feces stop in intestinal when
Between wait effect.Increase dietary fiber intake, can effectively preventing constipation, hemorrhoid prevent colon cancer, rectal cancer.Dietary fiber
The absorption of fat, cholesterol in intestinal can also be reduced, and promotes cholesterol and cholic acid to discharge from feces, thus have blood fat reducing,
The effect of cholesterol reducing.Additionally, the pectin in dietary fiber can extend the time of staying of the food in gastric, the suction of delay glucose
Speed is received, and reduces too high blood glucose, improve the symptom of diabetes.Increase the intake of dietary fiber, it is also fat with mitigating,
Breast Cancer Prevention ... waits effect ".It may be seen that the significance of diet fiber health-care effect.Correspondence China fitness-for-all shows
Shape and demand, can fully prove, dietary fiber has the huge social value for expediting the emergence of contemporary fitness-for-all.Ground according to analysis
Study carefully, if whole people's dietary fiber is in dietetic nutrition structure, keep the target of enough intakes, along with " three-hypers food " (high sugar,
Higher fatty acid, high protein) balance control and increase moderate exercise, then the sickness rate of China's nutrient imbalance metabolic disease can be
80% is at least reduced on the basis of existing.Especially Fructus Mori material further enhances immunologic function in intestinal, and resisting fatigue resists lean
Blood;The present invention is by a certain percentage added to the water the Fructus Mori of L-arabinose and oligosaccharide, makes functional sugar and Fructus Mori nutrient substance
Interact.Bacterium life group's growing environment in human body intestinal canal is lifted, the quantity of probiotic bacteria increases, Fructus Mori itself are again bacterium life all living creatures's length
Good medium, the back suction for adding L-arabinose receives the effect of sugared part, therefore, it can control people's blood glucose rise so that I
The sugared Fructus Mori compositionss of uncle in prevention diabetes, take a few days and intestinal beneficial flora can be allowed to breed at double, solves the problems, such as constipation, grow
Phase takes can substantially improve three high targets.
Formula according to embodiments of the present invention, body weight is close, the people of patient 8 with type Ⅱdiabetes mellitus without other medical histories
(blood glucose when before the meal in standard 50% or so, if do not taken hypoglycemic medicine), takes taken by the amount Consecutive Days of embodiment 1,2 after the meal
10 days, change of blood sugar was that, per reducing by more than 30% per capita, two blood glucose are completely normal.(blood glucose is before the meal in mark for the people of matched group two
When accurate 50% or so, if do not taken hypoglycemic medicine) unused this example 1,2, cause blood glucose to raise suddenly after the meal, deviate normal range.
The patient of type Ⅱdiabetes mellitus takes the consumption per day compositionss of embodiment 1,2 to the control effect of blood glucose more preferably.
Mice spleen lymphocytes proliferation ability of the present invention to ConA inductions, test sets the compositionss i.e. day of embodiment 1,2
Two dosage groups of consumption, negative control group is distilled water, using mtt assay, is as a result shown, high dose group can significantly improve mice
Spleen lymphocyte proliferation ability, surveys pouring and turns OD differences apparently higher than negative control group (P < 0.05).And low dose group and control
Group is to spleen lymphocyte proliferation DeGrain (P > 0.05).
The present invention can greatly enhance the antiinflammatory action of body itself using ferment, because ferment can make first cell
Compensation is strengthened, and especially gives inflammation part damaged cell power-assisted, greatly increases cell function excellent activity at this, so as to
Various compensations are conducive to give full play to antiinflammatory action.Phytic acid in plant is changed into into functional lactate and acetic acid, insoluble fibre
Dimension is changed into water soluble dietary fiber etc., affects more preferable to the positive of intestinal, and the product of preparation can be with various biologies in human activin
Enzyme, adjusts human metabolism function comprehensively.
Specific embodiment
The ferment of the present invention is made up of following methods, the probiotic bacteria 0.1-0.3 parts such as vegetable and fruit 25-60 part yeast,
0.2 part of Sal, water 35-100 parts the Fructus Mori of above-mentioned weight portion are accounted for 20-50% quality (especially more than 60% use Fructus Mori,
100% Fructus Mori can be adopted) raw material carries out first time fermentation, fermentation time 5 days, and fermentation room temperature is 20-28 DEG C, obtains one
Not good liquor and residue;Fructus Mori value is high, therefore the Dou Fructus Mori residues 20-32 parts that add water carry out second fermentation, and fermentation time 4 days is sent out
Ferment room temperature is 20-28 DEG C, and the mixing of the not good liquor of mulberry enzyme two is proceeded into maturation of fermenting, and fermentation maturation at least 70 days is obtained
Mulberry enzyme stock solution.
It is also conventional method that ferment adopts various vegetables together with fruit for raw material, and conventional also uses Herba Apii graveolentis, Fructus Citri Limoniae, kind
Eggplant, green tea, the fresh Fructus Chaenomeliss of addition, white turnip, Fructus Mali pumilae, pears etc., then the micro- life for adding Lactobacillus and Bei La yeast (main component)
Thing adds 1-2%, 25-35 DEG C of 7-25 days Production Time.Temperature is 20-25 DEG C of 25-45 days Production Time.
Concrete sweat is as follows:(1) it is saccharomycetic to activate with amplification culture aseptically, picking yeast seeds
On beef extract-peptone solid medium, after 35 DEG C of constant temperature culture 48h, stronger yeast of activity etc. is selected to mix bacterium in liquid
It is enlarged in culture medium and cultivates and carry out secondary spreading cultivation.
(2) cleaning of raw material takes out Fructus Mori in water after rinsed clean.
(3) fill basket and drain Fructus Mori fruit surface moisture in groove in special draining.
(4) crush barrelling carries out coarse pulverization by Fructus Mori, and granule is less than 2 millimeters.In the Fructus Mori for crushing add yeast,
Mel and Sal, mix, and are fitted in bucket after mineral water.Polypropylence Sheet is covered on the top of bucket, then is tightened with rubber case, and covered
Bung.
(5) fermentation of Fructus Mori and natural separation:Fermenter is put to the indoor fermentation of fermentation 5 days, the initial stage for carrying out Fructus Mori is sent out
Ferment, indoor temperature is controlled at 20-28 DEG C.
(6) shunting of the shunting one time fermentation liquid of mulberry enzyme:By menophania the fermenter that ferments transport to diffluence room, remove
Drop cloth, the cover plate for changing hole is covered on bung, bung is downwardly inclined on the splitter box for being placed in hole, above the hole of splitter box
Filter cloth is placed, mulberry enzyme liquid trickles naturally, realize being separated by filtration for mulberry enzyme liquid and residue, obtain mulberry enzyme once
Liquid.By the not good liquor of mulberry enzyme one being filtrated to get by pipeline, it is input in the tank body of fermentation ripening chamber with liquid-feeding pump;Two not good liquors
Shunting:Residue merging after one not good liquor is shunted is poured in bucket, adds mineral water to continue to be put into above-mentioned fermentation interior continuation
Fermentation, the time is 4 days, obtains the not good liquor of mulberry enzyme two.Two not good liquors and residue are separated by filtration.
(7) 36 DEG C of constant temperature of indoor maintenance of fermentation ripening chamber, by the liquid temperature that mulberry enzyme shunts liquid 38-40 DEG C is maintained,
Fermentation maturation at least 70 days, obtains mulberry enzyme stock solution.Mulberry enzyme stock solution fragrance pure taste, sugar≤6 g/l, total acid 12
G/l~17 g/l.The mulberry enzyme that the present invention is obtained remains to greatest extent the activity and function of effective ingredient, can be with
Unnecessary free radical, antioxidation in neutralization human body, slow down aging, alleviation eye strain, promotion cardiovascular health, reach beauty and support
The effect of face, building body, be a kind of good health product.
The present invention provides a kind of preparation method of plant enzyme, carry out plant enzyme natural extraction contain plant effectively into
The liquid for dividing, in maturation by fermentation, obtains liquid plant enzyme stock solution;The plant enzyme stock solution for obtaining contains multienzyme ferment
Element, proteolytic enzyme, lipase, amylolysis ferment, and containing proanthocyanidin, various natural complex, ore deposit
Material, aminoacid and trace element etc..With the plant enzyme stock solution for obtaining, plant enzyme liquid oral liquid, plant enzyme are made
Pressed powder or plant enzyme capsule.Preparation method of the present invention simulates the natural formation condition of ferment, and scientific in principle, equipment is simple
Large-scale production is suitable to, the plant enzyme pure natural feature for obtaining.Can be widely applied to health product or make up and cosmetics.
Edible fiber selects algae, the equator red algae Eucheuma muricatum (Gmel.) Web.Van Bos. such as Herba bromi japonici (skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, Thallus Laminariae (Thallus Eckloniae), Eucheuma muricatum (Gmel.) Web.Van Bos., bag
Include Equations of The Second Kind water soluble dietary fiber:Low fat pectin, high fat pectin, apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin,
Agar powder, carboxymethyl cellulose water soluble dietary fiber.
Arabinose enzyme composition has very strong decomposition.All biochemical reactions in human body, nearly all will be
Carry out under the catalytic action of arabinose enzyme composition, while impossible chemical reaction originally can be driven, and can
Do not transnatured with accelerating its chemolysis effect.Arabinose enzyme composition can cause human body cell for each simultaneously
The catabolic process for planting the vascular aggregates things such as metabolic waste, lipid peroxide and thrombosis greatly speeds up, useless in vivo so as to accelerate
The discharge of thing, toxin, the various toxin for eliminating internal long term accumulation and the foreign body for hindering blood normal circulation, are kept for human body generation
Thank to the unimpeded of normal and various circulations.
Embodiment 1-19 (mass unit:g)
In blood of human body contain hundreds of ferment, these ferment can be substantially strengthen blood in various cells activity and
Function, while removing waste and toxin in blood, purifies blood.Additionally, ferment is during waste in decomposition blood, can
By noxious substances such as a large amount of ammonia of the entrance blood produced by people's vivo protein catabolic process element (Ammonia), to become low
Toxicity is excreted from urine, so that blood is purified, becomes salubrious, and keeps optimal pH-value, is given full play to
The important function that blood is stopped to people.
Arabinose enzyme composition has good resistant function for various antibacterials and virus, inherently a kind of natural
Antibiotics.Arabinose enzyme composition catalysis biochemical reaction process in can produce one kind be called " ablastins " it is special because
Son, the factor pair antibacterial, virus have inhibitory action, just as the aerosol bomb of cuttle fish rear release under attack, form a kind of
" biological barrier " be enough to resist external enemy, so as to reduce hazardness of the bacterial viruses to human body.Research display, arabinose ferment
Promotor composition also has inhibitory action to harmful microorganism, and arabinose enzyme composition is to escherichia coli, Pseudomonas aeruginosa, gold
The inhibitory action of Staphylococcus aureus and three kinds of acne pathogen is confirmed.
Above-described specific embodiment, has been carried out further to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail, should be understood that the specific embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, all any modification, equivalent substitution and improvements within the spirit and principles in the present invention, done etc. all should include
Within protection scope of the present invention.
Claims (10)
1. arabinose enzyme composition, is characterized in that including ferment, and ferment is ferment prepared by vegetable or fruit raw material,
Quality formula is as follows:The L- arabinose of 3-25 parts, 3-15 part xylitol, ferment 10-200 parts.
2. arabinose enzyme composition according to claim 1, is characterized in that preparing ferment with more than 20% Fructus Mori quality
Element, and add 3-18 part oligosaccharide.
3. arabinose enzyme composition according to claim 2, is characterized in that adding water in compositionss, and the quality of water is
0.2-3 times of all substances quality.
4. arabinose enzyme composition according to claim 2, is characterized in that arabinose is extracted using biofermentation
L-arabinose of the purity up to more than 99.0%;L-arabinose:Xylitol constituent mass ratio is 1-1.4:1-4;Oligosaccharide
Including:Fructose oligosaccharides, polydextrose, GOS, oligomeric xylose, oligomeric isomaltose, oligomeric lactose, soy oligosaccharides
Sugar.
5. arabinose enzyme composition according to claim 1, is characterized in that ferment 30-60 parts;And add edible fine
Dimension 10-80 parts;Edible fiber selects Herba bromi japonici(Skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, Thallus Laminariae (Thallus Eckloniae), Eucheuma muricatum (Gmel.) Web.Van Bos., equator red algae, aqueous soluble dietary is fine
Dimension:Low fat pectin, high fat pectin, apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin, agar powder, carboxymethyl cellulose.
6. arabinose enzyme composition according to claim 1, it is characterized in that in arabinose compositionss L- I
The quality parts ratio of uncle's sugar, xylitol and oligomeric xylose is 5-10:5-10 :8-15.
7. the arabinose enzyme composition according to one of claim 1-6, is characterized in that adding edible selenium powder, add matter
Measure as 1-5 parts.
8. arabinose enzyme composition according to claim 7, is characterized in that quality of the ferment raw material using 20-50%
Fructus Mori, Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactobacillus and Bei La yeast addition 1-2%, 25-32 DEG C of Production Time 7-15
My god.
9. arabinose enzyme composition according to claim 7, is characterized in that adding fresh Fructus Chaenomeliss/or white in ferment raw material
Radix Raphani.
10. the preparation method of arabinose compositionss according to claim 7, is characterized in that prepared by ferment and completes;In water
Xylitol, L- arabinose and oligosaccharide, edible fiber are added, 80 DEG C of pasteurizing temperatures are warming up to, ferment is added after cooling
30-45min is incubated under element, stirring condition, that is, the bottling of arabinose compositionss is obtained.
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