CN106579387A - Arabinose-enzyme composition and preparation method thereof - Google Patents

Arabinose-enzyme composition and preparation method thereof Download PDF

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CN106579387A
CN106579387A CN201611167731.0A CN201611167731A CN106579387A CN 106579387 A CN106579387 A CN 106579387A CN 201611167731 A CN201611167731 A CN 201611167731A CN 106579387 A CN106579387 A CN 106579387A
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arabinose
ferment
enzyme composition
enzyme
parts
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余红
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Nanjing Sugar Prevention Biological Science And Technology Co Ltd
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Nanjing Sugar Prevention Biological Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

An arabinose-enzyme composition is prepared from an enzyme that is prepared from vegetable or/and fruit material, wherein by weight L-arabinose accounts for 3-25 parts, xylitol accounts for 3-15 parts, and the enzyme accounts for 10-200 parts. By mulberry fruit of 20% and above, the enzyme is prepared, and 3-18 parts of oligosaccharide is added. In the arabinose-enzyme composition, the mass ratio of L-arabinose, xylitol and oligosaccharide is (5-10):(5-10):(8-15), and 1-5 parts of edible selenium powder is added.

Description

A kind of arabinose enzyme composition and preparation method
Technical field
The present invention relates to a kind of health food, more particularly to a kind of arabinose enzyme composition and preparation.
Background technology
CN105285383 proposes a kind of Arabic syrup and preparation method thereof, and syrup includes water and saccharic composition, water and sugar Constituent mass volume ratio is 1~1.4:1;Saccharic composition is included by mass fraction:7~13 parts of L-arabinose, 5~9 parts of wood Sugar and 2~5 parts of oligomeric xylose.Saccharic composition is included by mass fraction:10~12 parts of L-arabinose, 6~8 parts of xylose and 2.5~3.5 parts of oligomeric xylose.But the function of L-arabinose, xylose, oligomeric xylose, than relatively similar, three kinds of functional sugars are mutual Effect is strong, especially the latter two functionally complete class it is same.It is beneficial to the quantity of flora in human body intestinal canal;Blood glucose can be controlled Raise, suppress lipogenesis so that arabinose Fructus Mori compositionss are in sides such as prevention diabetes, constipation, three-hypers and eliminating toxin and beautifying the skin Face has good effect, and long-term taking can allow intestinal beneficial flora to be bred at double.
The L-arabinose of arabinose especially main effect has been approved for new resources also known as Arabinose, pectose Food.Although the half of L-arabinose sugariness only sucrose, the saccharase of sucrose is digested in energy selectivity negatively influencing small intestinal, Suppress the absorption of sucrose so as to have obvious effect even directly to have a medical function at the control aspect such as blood glucose, the day of single 15g Dosing is even used to treat the type-II diabetes at initial stage.Oligosaccharide is except the physicochemical properties with general utility functions oligosaccharide Outward, most noticeable physiological property is that it can be obviously improved microbial population ratio in intestinal, and it is enteral bacillus bifiduss The activation and proliferation factor, for probiotic bacteria carbon source is provided, and is a class non-digestible oligosaccharide, not by gastric acid and digestion enzymatic degradation, directly Probiotic bacteria is preferably into big enteral to be utilized, while producing various organic acid, reduces gut pH, suppress harmful bacteria growing, Probiotic bacteria is set to breed in a large number in intestinal.The generation of enteral corrupt substance can be reduced and be suppressed, suppresses the growth of noxious bacteria, be adjusted Intestinal inner equilibrium;Trace elements iron, the absorption of calcium can be promoted and utilized, to prevent osteoporosis;Hepatotoxin, energy can be reduced The organic acid of anticancer is generated in intestinal, there is significant preventing cancer function;And pure taste is fragrant and sweet good to eat, with the adipose fragrance of class With tasty and refreshing soapy feeling.Typically have:Fructus Hordei Germinatus oligose glucose, fructose oligosaccharides, soybean oligo saccharide, polydextrose, gala Sugared oligosaccharide, oligofructose, oligomeric xylose (β-Isosorbide-5-Nitrae glycosidic bond combination).
Xylitol is a kind of sweeting agent with nutritive value, and sugariness is suitable with sucrose, is sucrose and Fructus Vitis viniferae sugar substitute. It is its a big feature that heat is low:2.4 calories per gram, than other little carbohydrates 40%.Welcome by diabetes patient very much. It is the intermediate of human body carbohydate metabolism, and insulin is lacked in vivo to be affected in the case of carbohydrate metabolism, to promote without the need for insulin, xylose Alcohol can also pass through cell membrane, be absorbed by tissue utilization, for cell with nutrition and energy, and blood glucose value will not be caused to raise, and eliminate Diabetes patient's symptom more than three (polyphagia, polydipsia, polyuria), is the sugar substitutes of the trophism for being best suitable for patients with diabetes mellitus, Sweeting agent, supplementary and the auxiliary therapeutical agent that diabetes patient can be is used.Xylitol can also improve liver function.It can promote Liver glycogen synthesizes, and blood glucose will not rise, and having to hepatopath improves liver function and lipotropic effect.Xylitol is also preventing decayed tooth The best sweeting agent of tooth, it is not produced cariogenic bacterial fermentation in oral cavity and is utilized, and suppresses the generation of streptococcus growth and acid;Energy Promote salivation, slow down pH value decline, reduce the acid etching of tooth, prevent dental caries and reduce the generation of dental plaque, can consolidate Tooth.Xylitol can reduce dental caries this characteristics, in high-risk rate crowd (dental caries incidence rate height, Undernutrition, oral hygiene Level is low) and low risk crowd (being taken care of one's teeth using current all of cleaning of teeth measure, tooth cavity generation rate is low) in be It is suitable for.Xylitol also has weight losing function.Energy is provided for human body, synthesizes glycogen, the protein in reduction fat and hepatic tissue Consume, liver is protected and is repaired, eliminate the generation that ketoboidies is harmful in human body, will not be worry of getting fat because edible.Wood Sugar alcohol biological stability is good, good hygroscopicity.
As the mankind are to health care and the requirement of auxiliary treatment diabetes, need to prepare one kind with health care, convenient food Arabinose compositionss.
Ferment refers to the biological effect active substance that bacterium is participated in from fermentation.Bacterium source ferment favourable to human body in prebiotic ferment Element includes:What active probiotic (probiotics) body, probiotic bacteria were produced is respectively enzyme (enzyme), prebiotic granulose (Lactobacilluspolysaccharide), lactein (Lactobacteriosin) four is most of.Active probiotic sheet Body, including lactic acid bacteria and Bei La yeast, are that national governments and linked groups of the United Nations allow the safe bacterial strain present in food. Have been widely used for various therapies.Generally comprise in prebiotic ferment:Lactobacillus acidophilus, Fructus rhamni (Rhamnus davurica Pall.) saccharobacillus, the bright coccus of a beading, Bifidobacterium adolescentiss, Lactobacillus plantarum, Bifidobacterium bifidum, Lu Shi lactic acid bacterias, being even more in reinforcing ferment includes 150 kinds of high activities Probiotic bacteria.The enzyme that probiotic bacteria is produced in raw ferment is exactly in fact Louis. the ferment (ferment) of Pasteur original definition.Including: Sour enzyme system is produced, is synthesized polysaccharide enzyme system, the enzyme system of decomposing nitrosamine, is reduced cholesterol enzyme system, control endotoxin enzyme system, reduces fat Enzyme system, synthetic vitamin enzyme system, decomposition cholic acid enzyme system, oxalic acid deshydroxy enzyme, superoxide dismutase.Prebiotic granulose in ferment (Lactobacillus polysaccharide), lactein (Lactobacteriosin) also serve as safe effect thing Matter, is being widely used in the fields such as treatment of cancer, food antiseptic.All prebiotic ferment all contain bacterium source ferment, therefore they Constitute the general utility functionss of prebiotic ferment.
The plant source ferment contained in prebiotic ferment, including:Enzyme (enzyme), phytochemistry element (Phytochemicals) two Big class.Enzyme in the ferment of plant source includes:Superoxide dismutase, Fructus Ananadis comosi protease, papain, Vc digestive enzyme, Fructus Fici disappear Change phytochemistry element point primary metabolite and secondary that enzyme, chitinase, oxalic acid deoxygenase, myrosin ... are planted in the ferment of source The class of metabolite two.According to chemical composition then include:Polyphenol, Saponin, polysaccharide, terpene, ketone, alkene, glycosides, phytosterol, phytohormone etc..In Magnificent national rarity, the traditional Chinese medical science and dietetic therapy, its core content is exactly rationally to be got rid of illness health preserving using these phytochemistry elements.
The content of the invention
It is an object of the present invention to provide a kind of arabinose enzyme composition and preparation method.Compositionss optimize intestinal with Ecology, be provided in particular in the fermented product such as Fructus Mori based on L-arabinose, xylitol and oligosaccharide in intestinal with microbial bacteria The reciprocal effect fallen with cyton, therefore, it is possible to have the healthy effect for more preferably adjusting human body.
The technical scheme is that, arabinose enzyme composition, including ferment, ferment is that vegetable or fruit are former Ferment prepared by material, quality formula is as follows:The L-arabinose of 3-25 parts, 3-15 part xylitol, ferment 10-200 parts.
With the ferment made above of Fructus Mori mass content 20%, and add 3-18 part oligosaccharide.
Semisolid can be prepared in compositionss, it is also possible to add water, the quality of water is the 0.2-3 of all substances quality Times.
L-arabinose of the purity that arabinose is extracted using biofermentation up to more than 99.0%;L-arabinose:Wood Sugar alcohol component mass ratio is 1-1.4:1-4;Oligosaccharide includes:Fructose oligosaccharides, polydextrose, GOS, oligomeric wood Sugar, oligomeric isomaltose, oligomeric lactose, soybean oligo saccharide.
Ferment 30-60 parts, and add edible fiber 10-80 parts.Edible fiber selects Herba bromi japonici (skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, sea The algae such as band, Eucheuma muricatum (Gmel.) Web.Van Bos., equator red algae Eucheuma muricatum (Gmel.) Web.Van Bos., including (Equations of The Second Kind) water soluble dietary fiber:Low fat pectin, high fat pectin, Apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin, agar powder, carboxymethyl cellulose water soluble dietary fiber.
The quality parts ratio of L-arabinose, xylitol and oligomeric xylose is 5-10 in arabinose compositionss:5-10:8- 15。
Described arabinose enzyme composition, then add edible selenium powder, addition quality is 1-5 parts.
Ferment raw material using 20-50% quality Fructus Mori, and using Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactobacillus and Shellfish draws yeast addition 1-2%, 25-32 DEG C of 7-25 days Production Time.
Ferment adds fresh Fructus Chaenomeliss and/or white turnip, or even the blue certain kind of berries.
The preparation method of described arabinose compositionss, adds xylitol, L-arabinose and oligosaccharide in water, heat up To 80 DEG C of pasteurizing temperatures, add after cooling and 30-45min is incubated under ferment, stirring condition, that is, arabinose compositionss are obtained Bottling.Addition 5-18 part oligosaccharide.Another addition water, the quality of water is 0.2-10 times of above-mentioned all substances quality.
Product of the purity that arabinose is extracted using biofermentation up to more than 99.0%.L-arabinose:Xylitol group Mass ratio is divided to be 1~1.4:1~4;
Ferment in particular by Fructus Mori 20-100% quality, other adopt Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactic acid bacteria drink Sour bacterium and Bei La yeast addition 1-2%, 25-32 DEG C of 7-25 days Production Time.Raw material can also add fresh Fructus Chaenomeliss, white turnip in ferment.
The preparation method of arabinose compositionss, ferment is prepared and completed;Xylitol, L-arabinose and oligomeric are added in water Sugar, edible fiber, are warming up to 80 DEG C of pasteurizing temperatures, add after cooling and 30-45min is incubated under ferment, stirring condition, that is, make Obtain the bottling of arabinose compositionss.
White turnip ferment can eliminate bodily waste rich in the amylase for decomposing harmful intestinal tract material, adjust intestinal group rings Border, keeps enteral various bacteria balance.The ferment that Fructus Lycopersici esculenti produces gives protection against cancer, antitumaous effect is more higher than lycopene.It is green in green tea Tea phenol is a kind of glutathione transferase, can increase the amount that Fructus Lycopersici esculenti produces ferment.Fructus Lycopersici esculenti ferment is to carcinoma of prostate and some digestion The preventive effect of road cancer it is further preferred that.7-25 days or longer Production Time.
Active lactobacillus and Bei La yeast, especially shellfish draw yeast production yeast selenium, add in yeast culture medium suitable The nothing of amount typically eats selenium powder yeast selenium, and organic selenium is produced to the accumulation ability of selenium using yeast, yeast is then peeled off, by nothing Breaking cellular wall is produced after machine selenium is removed.The existence form of selenium is more complicated in the constituent yeast selenium of yeast selenium, including seleno egg ammonia Acid, seleno Guang aminoacid, CH3SeH, selenoprotein and other selenides.Selenomethionine is also a kind of single selenium source.System After the completion of standby day oral dose deal addition L-arabinose.
Take:Every morning is drunk after 200 milliliters of warm waters on an empty stomach, drinks the ferment combination of 20-50 milliliters (or more) Arab Thing.The symptom for occurring similar " borborygmus/diarrhoea " during taking is that the intestines and stomach normally reacts.Suitable population:Malaise, Jing often feel Emit, drowsy, stomach QI-insufficiency, constipation, it is fat, like drinking, long-term smoking, depression person, three-hypers, have cancer family history etc.. Fermentable ferment is that, with raw materials such as one or more vegetable or fruit, what Jing multiple beneficials bacterium was fermented and produced gives birth to rich in dimension The functional microbial fermented food of the nutritional labelings such as element, enzyme, mineral and secondary metabolite.Enzyme microb have delay The plurality of health care functions such as aging, anti-inflammation, purification blood, enhancing human body immunity ability and detoxicating and fighting cancer.Fermentation is mainly profit With polysaccharide in the presence of yeast, ethanol, carbon dioxide, water and aromatic substance are decomposed into, so as to prepared flavour is sour-sweet, mouthfeel The mulberry enzyme of sweet-smelling.The antioxygenic property of ferment is fermented essentially from sweat, Fructus Mori in the presence of microorganism, Organic acid concentration increase in fermentation liquid makes the pH value of fermentation liquid 4 or so, and sour environment causes protein to be hydrolyzed to aminoacid.With This simultaneously, yeast will vegetable fruit expect in enrich and complicated macromole aldehydes matter change into small molecule, total phenol content liter Height, aldehydes matter is easy to provide a hydrion and stablize by resonance hybrid, makes mulberry enzyme DPPH free radical scavenging Ability is significantly improved.In addition, the polyhydroxy on the A rings and B rings of chromocor compound molecule replaces and the 3- hydroxyls of free radical replace Base can strengthen the Scavenging activity of the superoxide radical of ferment.Meanwhile, mulberry enzyme antioxidant content during the fermentation increases Plus, its reducing power is significantly enhanced.
Enzyme microb is that fermentation of the active probiotic body to vegetable and fruit is obtained, and active probiotic mainly includes lactic acid Bacterium and Bei La yeast, are that national governments and linked groups of the United Nations allow the safe bacterial strain present in food.Extensively used In various therapies.Also include in prebiotic ferment:Lactobacillus acidophilus, Fructus rhamni (Rhamnus davurica Pall.) saccharobacillus, the bright coccus of a beading, youth bifid bar Bacterium, Lactobacillus plantarum, Bifidobacterium bifidum, Lu Shi lactic acid bacterias, being even more in reinforcing ferment includes that more than 100 kinds of high activity is prebiotic Bacterium colony.Ferment not only make use of the plant source ferment contained in veterinary antibiotics, and carry out attenuation synergistic to edible composition.Cause This has very magical biological effect.Specifically this kind of plant source ferment has:Sulforaphane, resveratrol, ginsenoside, spirit Sesame polysaccharide, anthocyanidin, limonin, bitter melon polysaccharide, gingerol, isoflavone, hydroxymalonic acid.
The preparation method of the composite plant ferment containing profitable probliotics, has selected the various fruits vegetable, medicine food dual purpose plant to be Fermentation substrate, and using various probiotic bacteria/yeast as fermentation inoculum, using multicycle discontinuous fed-batch fermentation, the multistage depths of Jing After layer fermentation, the composite plant ferment containing profitable probliotics is prepared.The species of the enzyme that prepared composite plant ferment contains Many, activity is high, and good stability is affected by environment little.Using this method different time sections using the different substrates of different strain fermentation, Make the different extracts active ingredients contained by all kinds of fermentation substrates more complete, whole system fermentation is more fully coordinated.Ferment Element constitutes total performance of human life phenomenon in catalysis in vivo thousands of kinds of biochemical reactions.Machine can comprehensively be controlled and optimized to ferment Internal milieu so as to keep dynamic equilibrium, that is, the equilibrium between yin and yang state described in the traditional Chinese medical science, is for healthy state.Ferment is in room temperature In oxidation reaction produced by energy, and the storage and utilization of the energy, showing as ferment can enhance metabolism, and adjust Section secretion, improves digestive function, and metabolic waste and toxin are eliminated in time, promotes the raw bacteria growing that overflows in intestinal, and keeps intestinal The good intestinal environment of bacterial micro-ecological balance.Ferment carries out incessantly from many aspects internal efforts at environmental protection, makes human body dirty Internal organs function keeps optimum state, improves body constitution, disease-prevention health health.Ferment can improve and maintain the normal bacterium phase of intestinal, have bowel relieving to arrange Toxic action, it is possible to reduce enteral noxious substance is entered in blood, to keep blood pure.
Beneficial effect:Due to the absorption to sugar of the intestinal that has its source in of hyperglycemia, undue absorption glucose enters blood Liquid causes insulin resistance or opposing, modal insulin resistance type and a kind of condition of illness contact for being referred to as metabolism syndrome Together.Insulin resistance can develop into thoroughly type-II diabetes.Commonly post prandial hyperglycemia, in such case Under, pancreas beta cell cannot produce enough insulins to keep normal glycemic levels.Beta cell nothing in the case of hyperglycemia It is the feature changed from insulin resistance to type-II diabetes that power secretes more insulins.Emphasis is aimed at this by the present invention, Optimization intestinal and ecology, comprehensive mechanism is more complicated, it should which Fructus Mori based on L-arabinose and oligosaccharide etc. are in intestinal With microbe colony and the reciprocal effect of cyton, somatic cell is excited in beneficial agents, stimulates beneficial to produce, Mulberry on surface Shen etc. and oligosaccharide, fiber can promote the wriggling of intestinal, and more deep effect is nutrition and strong intestinal cell, is had in absorption Beneficial excitor substance (enzyme, peptide and small protein including producing), therefore, it is possible to adjust the immunity of human body, although on surface Defecation is significantly improved, but the malicious and net blood of Eradicates more has influence on the health of human liver, so as to also remote-effects arrive kidney etc..Especially Intestinal hinders absorption of the intestinal to multitudinous sugar because of L-arabinose, and the nutrition that glycogen becomes probiotic bacteria again is former, more have stimulated probiotic bacteria Secretion beneficial products.
For from the origin of life, intestinal is the foundation of acquired constitution, and the intestinal of health is to absorb transporting food and various nutrition The nutrition of product, the intestinal of the health immune organ that to be again function maximum with volume, the present invention based on the nutrition of intestinal, nourishing and Health care can fully improve the transporting ability of intestinal.
Point out when dietary fiber is spoken of in health:" ... dietary fiber plays in vivo critical function, act as health The role of bodyguard.Dietary fiber has a stimulation intestinal peristalsis promoting, increases the volume of intestinal contentses, reduce that feces stop in intestinal when Between wait effect.Increase dietary fiber intake, can effectively preventing constipation, hemorrhoid prevent colon cancer, rectal cancer.Dietary fiber The absorption of fat, cholesterol in intestinal can also be reduced, and promotes cholesterol and cholic acid to discharge from feces, thus have blood fat reducing, The effect of cholesterol reducing.Additionally, the pectin in dietary fiber can extend the time of staying of the food in gastric, the suction of delay glucose Speed is received, and reduces too high blood glucose, improve the symptom of diabetes.Increase the intake of dietary fiber, it is also fat with mitigating, Breast Cancer Prevention ... waits effect ".It may be seen that the significance of diet fiber health-care effect.Correspondence China fitness-for-all shows Shape and demand, can fully prove, dietary fiber has the huge social value for expediting the emergence of contemporary fitness-for-all.Ground according to analysis Study carefully, if whole people's dietary fiber is in dietetic nutrition structure, keep the target of enough intakes, along with " three-hypers food " (high sugar, Higher fatty acid, high protein) balance control and increase moderate exercise, then the sickness rate of China's nutrient imbalance metabolic disease can be 80% is at least reduced on the basis of existing.Especially Fructus Mori material further enhances immunologic function in intestinal, and resisting fatigue resists lean Blood;The present invention is by a certain percentage added to the water the Fructus Mori of L-arabinose and oligosaccharide, makes functional sugar and Fructus Mori nutrient substance Interact.Bacterium life group's growing environment in human body intestinal canal is lifted, the quantity of probiotic bacteria increases, Fructus Mori itself are again bacterium life all living creatures's length Good medium, the back suction for adding L-arabinose receives the effect of sugared part, therefore, it can control people's blood glucose rise so that I The sugared Fructus Mori compositionss of uncle in prevention diabetes, take a few days and intestinal beneficial flora can be allowed to breed at double, solves the problems, such as constipation, grow Phase takes can substantially improve three high targets.
Formula according to embodiments of the present invention, body weight is close, the people of patient 8 with type Ⅱdiabetes mellitus without other medical histories (blood glucose when before the meal in standard 50% or so, if do not taken hypoglycemic medicine), takes taken by the amount Consecutive Days of embodiment 1,2 after the meal 10 days, change of blood sugar was that, per reducing by more than 30% per capita, two blood glucose are completely normal.(blood glucose is before the meal in mark for the people of matched group two When accurate 50% or so, if do not taken hypoglycemic medicine) unused this example 1,2, cause blood glucose to raise suddenly after the meal, deviate normal range. The patient of type Ⅱdiabetes mellitus takes the consumption per day compositionss of embodiment 1,2 to the control effect of blood glucose more preferably.
Mice spleen lymphocytes proliferation ability of the present invention to ConA inductions, test sets the compositionss i.e. day of embodiment 1,2 Two dosage groups of consumption, negative control group is distilled water, using mtt assay, is as a result shown, high dose group can significantly improve mice Spleen lymphocyte proliferation ability, surveys pouring and turns OD differences apparently higher than negative control group (P < 0.05).And low dose group and control Group is to spleen lymphocyte proliferation DeGrain (P > 0.05).
The present invention can greatly enhance the antiinflammatory action of body itself using ferment, because ferment can make first cell Compensation is strengthened, and especially gives inflammation part damaged cell power-assisted, greatly increases cell function excellent activity at this, so as to Various compensations are conducive to give full play to antiinflammatory action.Phytic acid in plant is changed into into functional lactate and acetic acid, insoluble fibre Dimension is changed into water soluble dietary fiber etc., affects more preferable to the positive of intestinal, and the product of preparation can be with various biologies in human activin Enzyme, adjusts human metabolism function comprehensively.
Specific embodiment
The ferment of the present invention is made up of following methods, the probiotic bacteria 0.1-0.3 parts such as vegetable and fruit 25-60 part yeast, 0.2 part of Sal, water 35-100 parts the Fructus Mori of above-mentioned weight portion are accounted for 20-50% quality (especially more than 60% use Fructus Mori, 100% Fructus Mori can be adopted) raw material carries out first time fermentation, fermentation time 5 days, and fermentation room temperature is 20-28 DEG C, obtains one Not good liquor and residue;Fructus Mori value is high, therefore the Dou Fructus Mori residues 20-32 parts that add water carry out second fermentation, and fermentation time 4 days is sent out Ferment room temperature is 20-28 DEG C, and the mixing of the not good liquor of mulberry enzyme two is proceeded into maturation of fermenting, and fermentation maturation at least 70 days is obtained Mulberry enzyme stock solution.
It is also conventional method that ferment adopts various vegetables together with fruit for raw material, and conventional also uses Herba Apii graveolentis, Fructus Citri Limoniae, kind Eggplant, green tea, the fresh Fructus Chaenomeliss of addition, white turnip, Fructus Mali pumilae, pears etc., then the micro- life for adding Lactobacillus and Bei La yeast (main component) Thing adds 1-2%, 25-35 DEG C of 7-25 days Production Time.Temperature is 20-25 DEG C of 25-45 days Production Time.
Concrete sweat is as follows:(1) it is saccharomycetic to activate with amplification culture aseptically, picking yeast seeds On beef extract-peptone solid medium, after 35 DEG C of constant temperature culture 48h, stronger yeast of activity etc. is selected to mix bacterium in liquid It is enlarged in culture medium and cultivates and carry out secondary spreading cultivation.
(2) cleaning of raw material takes out Fructus Mori in water after rinsed clean.
(3) fill basket and drain Fructus Mori fruit surface moisture in groove in special draining.
(4) crush barrelling carries out coarse pulverization by Fructus Mori, and granule is less than 2 millimeters.In the Fructus Mori for crushing add yeast, Mel and Sal, mix, and are fitted in bucket after mineral water.Polypropylence Sheet is covered on the top of bucket, then is tightened with rubber case, and covered Bung.
(5) fermentation of Fructus Mori and natural separation:Fermenter is put to the indoor fermentation of fermentation 5 days, the initial stage for carrying out Fructus Mori is sent out Ferment, indoor temperature is controlled at 20-28 DEG C.
(6) shunting of the shunting one time fermentation liquid of mulberry enzyme:By menophania the fermenter that ferments transport to diffluence room, remove Drop cloth, the cover plate for changing hole is covered on bung, bung is downwardly inclined on the splitter box for being placed in hole, above the hole of splitter box Filter cloth is placed, mulberry enzyme liquid trickles naturally, realize being separated by filtration for mulberry enzyme liquid and residue, obtain mulberry enzyme once Liquid.By the not good liquor of mulberry enzyme one being filtrated to get by pipeline, it is input in the tank body of fermentation ripening chamber with liquid-feeding pump;Two not good liquors Shunting:Residue merging after one not good liquor is shunted is poured in bucket, adds mineral water to continue to be put into above-mentioned fermentation interior continuation Fermentation, the time is 4 days, obtains the not good liquor of mulberry enzyme two.Two not good liquors and residue are separated by filtration.
(7) 36 DEG C of constant temperature of indoor maintenance of fermentation ripening chamber, by the liquid temperature that mulberry enzyme shunts liquid 38-40 DEG C is maintained, Fermentation maturation at least 70 days, obtains mulberry enzyme stock solution.Mulberry enzyme stock solution fragrance pure taste, sugar≤6 g/l, total acid 12 G/l~17 g/l.The mulberry enzyme that the present invention is obtained remains to greatest extent the activity and function of effective ingredient, can be with Unnecessary free radical, antioxidation in neutralization human body, slow down aging, alleviation eye strain, promotion cardiovascular health, reach beauty and support The effect of face, building body, be a kind of good health product.
The present invention provides a kind of preparation method of plant enzyme, carry out plant enzyme natural extraction contain plant effectively into The liquid for dividing, in maturation by fermentation, obtains liquid plant enzyme stock solution;The plant enzyme stock solution for obtaining contains multienzyme ferment Element, proteolytic enzyme, lipase, amylolysis ferment, and containing proanthocyanidin, various natural complex, ore deposit Material, aminoacid and trace element etc..With the plant enzyme stock solution for obtaining, plant enzyme liquid oral liquid, plant enzyme are made Pressed powder or plant enzyme capsule.Preparation method of the present invention simulates the natural formation condition of ferment, and scientific in principle, equipment is simple Large-scale production is suitable to, the plant enzyme pure natural feature for obtaining.Can be widely applied to health product or make up and cosmetics.
Edible fiber selects algae, the equator red algae Eucheuma muricatum (Gmel.) Web.Van Bos. such as Herba bromi japonici (skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, Thallus Laminariae (Thallus Eckloniae), Eucheuma muricatum (Gmel.) Web.Van Bos., bag Include Equations of The Second Kind water soluble dietary fiber:Low fat pectin, high fat pectin, apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin, Agar powder, carboxymethyl cellulose water soluble dietary fiber.
Arabinose enzyme composition has very strong decomposition.All biochemical reactions in human body, nearly all will be Carry out under the catalytic action of arabinose enzyme composition, while impossible chemical reaction originally can be driven, and can Do not transnatured with accelerating its chemolysis effect.Arabinose enzyme composition can cause human body cell for each simultaneously The catabolic process for planting the vascular aggregates things such as metabolic waste, lipid peroxide and thrombosis greatly speeds up, useless in vivo so as to accelerate The discharge of thing, toxin, the various toxin for eliminating internal long term accumulation and the foreign body for hindering blood normal circulation, are kept for human body generation Thank to the unimpeded of normal and various circulations.
Embodiment 1-19 (mass unit:g)
In blood of human body contain hundreds of ferment, these ferment can be substantially strengthen blood in various cells activity and Function, while removing waste and toxin in blood, purifies blood.Additionally, ferment is during waste in decomposition blood, can By noxious substances such as a large amount of ammonia of the entrance blood produced by people's vivo protein catabolic process element (Ammonia), to become low Toxicity is excreted from urine, so that blood is purified, becomes salubrious, and keeps optimal pH-value, is given full play to The important function that blood is stopped to people.
Arabinose enzyme composition has good resistant function for various antibacterials and virus, inherently a kind of natural Antibiotics.Arabinose enzyme composition catalysis biochemical reaction process in can produce one kind be called " ablastins " it is special because Son, the factor pair antibacterial, virus have inhibitory action, just as the aerosol bomb of cuttle fish rear release under attack, form a kind of " biological barrier " be enough to resist external enemy, so as to reduce hazardness of the bacterial viruses to human body.Research display, arabinose ferment Promotor composition also has inhibitory action to harmful microorganism, and arabinose enzyme composition is to escherichia coli, Pseudomonas aeruginosa, gold The inhibitory action of Staphylococcus aureus and three kinds of acne pathogen is confirmed.
Above-described specific embodiment, has been carried out further to the purpose of the present invention, technical scheme and beneficial effect Describe in detail, should be understood that the specific embodiment that the foregoing is only the present invention, be not intended to limit the present invention Protection domain, all any modification, equivalent substitution and improvements within the spirit and principles in the present invention, done etc. all should include Within protection scope of the present invention.

Claims (10)

1. arabinose enzyme composition, is characterized in that including ferment, and ferment is ferment prepared by vegetable or fruit raw material, Quality formula is as follows:The L- arabinose of 3-25 parts, 3-15 part xylitol, ferment 10-200 parts.
2. arabinose enzyme composition according to claim 1, is characterized in that preparing ferment with more than 20% Fructus Mori quality Element, and add 3-18 part oligosaccharide.
3. arabinose enzyme composition according to claim 2, is characterized in that adding water in compositionss, and the quality of water is 0.2-3 times of all substances quality.
4. arabinose enzyme composition according to claim 2, is characterized in that arabinose is extracted using biofermentation L-arabinose of the purity up to more than 99.0%;L-arabinose:Xylitol constituent mass ratio is 1-1.4:1-4;Oligosaccharide Including:Fructose oligosaccharides, polydextrose, GOS, oligomeric xylose, oligomeric isomaltose, oligomeric lactose, soy oligosaccharides Sugar.
5. arabinose enzyme composition according to claim 1, is characterized in that ferment 30-60 parts;And add edible fine Dimension 10-80 parts;Edible fiber selects Herba bromi japonici(Skin), Testa Fagopyri Esculenti, Rhizoma amorphophalli, Thallus Laminariae (Thallus Eckloniae), Eucheuma muricatum (Gmel.) Web.Van Bos., equator red algae, aqueous soluble dietary is fine Dimension:Low fat pectin, high fat pectin, apple pectin, pomelo-pectin, blue berry pectin, Fructus Ananadis comosi pectin, agar powder, carboxymethyl cellulose.
6. arabinose enzyme composition according to claim 1, it is characterized in that in arabinose compositionss L- I The quality parts ratio of uncle's sugar, xylitol and oligomeric xylose is 5-10:5-10 :8-15.
7. the arabinose enzyme composition according to one of claim 1-6, is characterized in that adding edible selenium powder, add matter Measure as 1-5 parts.
8. arabinose enzyme composition according to claim 7, is characterized in that quality of the ferment raw material using 20-50% Fructus Mori, Fructus Citri Limoniae, Fructus Lycopersici esculenti, green tea, then add Lactobacillus and Bei La yeast addition 1-2%, 25-32 DEG C of Production Time 7-15 My god.
9. arabinose enzyme composition according to claim 7, is characterized in that adding fresh Fructus Chaenomeliss/or white in ferment raw material Radix Raphani.
10. the preparation method of arabinose compositionss according to claim 7, is characterized in that prepared by ferment and completes;In water Xylitol, L- arabinose and oligosaccharide, edible fiber are added, 80 DEG C of pasteurizing temperatures are warming up to, ferment is added after cooling 30-45min is incubated under element, stirring condition, that is, the bottling of arabinose compositionss is obtained.
CN201611167731.0A 2016-12-16 2016-12-16 Arabinose-enzyme composition and preparation method thereof Pending CN106579387A (en)

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CN107898762A (en) * 2017-11-21 2018-04-13 闽南师范大学 A kind of shaddock ped cellulose tablet and preparation method thereof
CN108157780A (en) * 2018-02-09 2018-06-15 叶小勇 A kind of food for assisting control body weight and preparation method thereof
CN108813594A (en) * 2018-07-02 2018-11-16 杭州相生相成科技有限公司 A kind of Ma Qiguo ferment and its compounding method
CN109043259A (en) * 2018-07-31 2018-12-21 天津市圭谷科技发展有限公司 A kind of ferment L-arabinose stachyose composite beverage underflow
CN109170875A (en) * 2018-08-27 2019-01-11 山东福田药业有限公司 A kind of ferment 3D printing food material and preparation method thereof
CN109198335A (en) * 2018-09-19 2019-01-15 杭州德能生物医药有限公司 The formula of plant enzyme draft drink
CN109527545A (en) * 2018-12-01 2019-03-29 山东先大健康产业股份有限公司 The formula and preparation method of a kind of L-arabinose, ferment and Freeze-dry Powder of Probioctics
CN110037287A (en) * 2019-04-26 2019-07-23 山东福田药业有限公司 A kind of mulberry enzyme powder and preparation method thereof
CN113907233A (en) * 2021-10-14 2022-01-11 厦门绿泗海生物科技有限公司 Probiotic fruit juice and preparation process thereof
CN115153011A (en) * 2022-07-04 2022-10-11 广州正明后生元科技有限公司 A metabiotic composition for regulating gastrointestinal tract, and its preparation method

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107898762A (en) * 2017-11-21 2018-04-13 闽南师范大学 A kind of shaddock ped cellulose tablet and preparation method thereof
CN107898762B (en) * 2017-11-21 2020-06-09 闽南师范大学 Shaddock peel cellulose tablet and preparation method thereof
CN108157780A (en) * 2018-02-09 2018-06-15 叶小勇 A kind of food for assisting control body weight and preparation method thereof
CN108813594A (en) * 2018-07-02 2018-11-16 杭州相生相成科技有限公司 A kind of Ma Qiguo ferment and its compounding method
CN109043259A (en) * 2018-07-31 2018-12-21 天津市圭谷科技发展有限公司 A kind of ferment L-arabinose stachyose composite beverage underflow
CN109170875A (en) * 2018-08-27 2019-01-11 山东福田药业有限公司 A kind of ferment 3D printing food material and preparation method thereof
CN109198335A (en) * 2018-09-19 2019-01-15 杭州德能生物医药有限公司 The formula of plant enzyme draft drink
CN109527545A (en) * 2018-12-01 2019-03-29 山东先大健康产业股份有限公司 The formula and preparation method of a kind of L-arabinose, ferment and Freeze-dry Powder of Probioctics
CN110037287A (en) * 2019-04-26 2019-07-23 山东福田药业有限公司 A kind of mulberry enzyme powder and preparation method thereof
CN110037287B (en) * 2019-04-26 2022-06-10 山东福田药业有限公司 Mulberry enzyme powder and preparation method thereof
CN113907233A (en) * 2021-10-14 2022-01-11 厦门绿泗海生物科技有限公司 Probiotic fruit juice and preparation process thereof
CN115153011A (en) * 2022-07-04 2022-10-11 广州正明后生元科技有限公司 A metabiotic composition for regulating gastrointestinal tract, and its preparation method

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