CN107549792A - A kind of sea cucumber peptide composite nutrition powder and its preparation technology - Google Patents
A kind of sea cucumber peptide composite nutrition powder and its preparation technology Download PDFInfo
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Abstract
The invention belongs to health products manufacture field, and in particular to a kind of sea cucumber peptide composite nutrition powder and its preparation technology.Described sea cucumber peptide composite nutrition powder, including sea cucumber polypeptide powder and composite plant ferment powder, described composite plant ferment powder, raw material include jujube, mulberries, fig.Select the fruit fermentation that jujube, mulberries and fig enzyme activity are abundant, after microbial fermentation, macro-molecular protein is decomposed, is converted into the small molecule nutrient such as a variety of essential amino acids, nucleic acid, combined simultaneously with the sea cucumber Gly-His-Lys of small-molecular-weight, improve absorptivity, it is easy to human consumption's absorption, promotes human metabolism.Described sea cucumber peptide composite nutrition powder has the effect of antifatigue, enhancing immunity of organisms, anti-aging, promotes wound healing effect notable.
Description
Technical field
The invention belongs to health products manufacture field, more particularly to a kind of sea cucumber peptide composite nutrition powder and its preparation technology.
Background technology
Sea cucumber is typical high protein, low fat, extremely low cholesterol, the food rich in minerals and vitamins, is had very
High healthcare function and medical value, is listed in " one of eight delicacies ".Collagen in sea cucumber accounts for the 70% of body wall, sea cucumber
Peptide is resulting product after being degraded by holothurian collagen, it is easier to utilization can be just absorbed by organisms, it is without any side effects, and can
With long-term consumption.
Ferment is also known as enzyme, is a kind of active macromolecules with catalytic action, the various metabolic activities in participant's body.
Ferment is present in all living cells, is that it starts the vigor of cell, cell is shown a variety of biological phenomenas, if without ferment
Element, ovum, sperm can not just combine, and plant can not carry out photosynthesis, and all cells will all run out of steam, biological phenomena also without
From generation, so all living things all be unable to do without ferment.All contain ferment in any organism, no matter plant or animal, vegetables
Have inside fruit, also have inside meat.But directly eat raw or prepared food can not all make nutrition be fully used.
The content of the invention
The present invention provides a kind of sea cucumber peptide composite nutrition powder and its preparation technology, including sea cucumber Gly-His-Lys and composite plant ferment
Powder, composite plant ferment powder is made by fermenting, successfully solves the problems, such as that human body can not make full use of plant endo enzyme, carries
High human body carries out scientific compatibility to plant nutrient absorptivity, while by sea cucumber Gly-His-Lys and composite plant ferment powder, has anti-tired
The effect of labor, enhancing immunity of organisms, anti-aging, promote wound healing effect notable.
To solve above technical problem, the technical solution adopted by the present invention is：
A kind of sea cucumber peptide composite nutrition powder of the present invention, including sea cucumber Gly-His-Lys and composite plant ferment powder；Described
Composite plant yeast powder, raw material include jujube, mulberries, fig.
Optionally, jujube, mulberries, the ratio of weight and number of fig are in the composite plant ferment powder raw material：
The Hong Zao ﹕ Sang Shen ﹕ fig=20~﹕ 25~60 of 45 ﹕ 20~30.
The preparation technology of sea cucumber peptide composite nutrition powder of the present invention, step include：
1) preparation of sea cucumber Gly-His-Lys
By sea cucumber preprocessing, sea cucumber enzymolysis, alcohol precipitation, concentration, spray drying, sea cucumber Gly-His-Lys are obtained；
2) preparation of composite plant ferment powder
Jujube, mulberries, fig are mixed, homogenate, composite plant enzymolysis, ferment, spray drying, obtain composite plant ferment
Powder；
3) allocate
Sea cucumber Gly-His-Lys are well mixed with composite plant ferment powder.
Optionally, in step 1), the step of sea cucumber digests, is 3~6wt% compound protease and the compound wind of 2~4wt%
Taste protease, 40~50 DEG C of temperature, 4~6h of enzymolysis time.
Optionally, it with weight, the concentration of 1~2 times of sea cucumber hydrolysate is 70~80% to be in step 1), the step of alcohol precipitation
Ethanol, temperature is at 2~10 DEG C, and 8~16h of time, separating to precipitate.
Optionally, in step 2), composite plant digest the step of be 0.5~1wt% zytases and 1~3wt% Novi
Believe enzyme, pH is 5.5~6.5, and temperature is 45~55 DEG C, and enzymolysis time is 2~4h, is concentrated to soluble solid content and is
20wt%, obtain composite plant concentration enzymolysis liquid.
Optionally, it is sterilizing the step of fermentation in step 2)；Saccharomycetes to make fermentation；Lactobacillus-fermented.
Optionally, the step of saccharomycetes to make fermentation is inoculation 0.1~0.3wt% saccharomycete, in the case of ensureing abundant dissolved oxygen,
30~35 DEG C of 12~16h of fermentation.
Optionally, the step of lactobacillus-fermented is to be inoculated with 0.5~1.5wt% lactic acid bacterias, 37~40 DEG C of 12~18h of fermentation.
Optionally, in step 3), sea cucumber Gly-His-Lys are with composite plant ferment powder percentage by weight：The compound plants of sea cucumber peptide Fen ﹕
The ﹕ 35~45 of thing ferment powder=55~65.
Compared with prior art, the invention has the advantages that：
1) sea cucumber peptide composite nutrition powder of the invention, including sea cucumber Gly-His-Lys and composite plant ferment powder, sea cucumber Gly-His-Lys are behaved
Body metabolism provides high-quality raw material, and composite plant ferment powder has the function that to enhance metabolism, the two effect compatibility, tool
There is the effect of antifatigue, enhancing immunity of organisms, anti-aging, promote wound healing effect notable.
2) the fruit fermentation that jujube, mulberries and fig enzyme activity are abundant is selected, not only by contained unhelpful material meeting
It is decomposed by the microorganisms, lengthy fermentation can more improve decomposition digestibility of the microorganism beneficial bacterium to raw material nutrient, will can divide greatly
Sub- breaks down proteins, the small molecule nutrient such as a variety of essential amino acids, nucleic acid is converted into, improves human body to plant enzyme nutrition
Absorptivity, it is easier to which human body is converted and absorbed.
Embodiment
Technical solution of the present invention is described in detail with reference to specific embodiment.
Embodiment 1
Formula composition：
The raw material of composite plant ferment powder is jujube, mulberries, wherein fig, 35 parts of jujube, 25 parts of mulberries, fig 45
Part.
The preparation technology of sea cucumber peptide composite nutrition powder is：
1) preparation of sea cucumber Gly-His-Lys
Sea cucumber is soaked, cleans the water for removing 2 times of silt, crushing plus sea cucumber weight, adds 4.5wt% compound protease
With 3.0wt% compound fertilizer productions, 45 DEG C of enzymolysis 5h, high-temperature boiling, sea cucumber hydrolysate is obtained；With 1.5 times of sea cucumber hydrolysate
Weight, the ethanol that concentration is 75%, temperature is at 6 DEG C, time 12h, alcohol precipitation；Concentration, spray drying, obtains sea cucumber Gly-His-Lys.
2) preparation of composite plant ferment powder
Take jujube, mulberries, fig to mix in proportion, be homogenized；From 0.7wt% zytases and 2wt% Novi letter enzyme
Carry out double enzymolysis, pH 6.0, temperature 50 C, enzymolysis time 3h；It is 20% that enzymolysis liquid is concentrated into soluble solid content,
Obtain composite plant concentration enzymolysis liquid；After sterilizing, 0.2wt% saccharomycete is inoculated with, in the case where 33 DEG C ensure abundant dissolved oxygen, is carried out
Saccharomycetes to make fermentation 14h；1.0wt% lactic acid bacterias are inoculated with, in 38 DEG C of standings, carry out lactobacillus-fermented 15h；Spray drying, is obtained compound
Plant enzyme powder.
3) dispensing
By sea cucumber Gly-His-Lys and composite plant ferment powder, by weight percentage for：Sea cucumber peptide Fen ﹕ composite plants ferment powder=
60 ﹕ 40, it is well mixed, obtains sea cucumber peptide composite nutrition powder.
4) dispense
Sea cucumber peptide composite nutrition powder is pressed into 10g/ bags, pouch sealing.
Embodiment 2
Formula composition：
The raw material of composite plant ferment powder is jujube, mulberries, wherein fig, 20 parts of jujube, 20 parts of mulberries, fig 25
Part.
The preparation technology of sea cucumber peptide composite nutrition powder is：
1) preparation of sea cucumber Gly-His-Lys
Sea cucumber is soaked, cleans the water for removing 2 times of silt, crushing plus sea cucumber weight, the compound protease and 2% for adding 3 are answered
Flavor protease is closed, 40 DEG C of enzymolysis 4h, high-temperature boiling, obtains sea cucumber hydrolysate；It is with the weight of 1 times of sea cucumber hydrolysate, concentration
70% ethanol, temperature is at 2 DEG C, time 8h, alcohol precipitation；Concentration, spray drying, obtains sea cucumber Gly-His-Lys.
3) preparation of composite plant ferment powder
Take jujube, mulberries, fig to mix in proportion, be homogenized；From 0.5wt% zytases and 1wt% Novi letter enzyme
Carry out double enzymolysis, pH 5.5, temperature 45 C, enzymolysis time 2h；Enzymolysis liquid is concentrated into soluble solid content is
20wt%, obtain composite plant concentration enzymolysis liquid；After sterilizing, 0.1wt% saccharomycete is inoculated with, ensures abundant dissolved oxygen at 30~35 DEG C
In the case of, carry out saccharomycetes to make fermentation 12h；0.5wt% lactic acid bacterias are inoculated with, in 37 DEG C of standings, carry out lactobacillus-fermented 12h；Spraying
Dry, obtain composite plant ferment powder.
3) dispensing
By sea cucumber Gly-His-Lys and composite plant ferment powder, by weight percentage for：Sea cucumber peptide Fen ﹕ composite plants ferment powder=
55 ﹕ 35, it is well mixed, obtains sea cucumber peptide composite nutrition powder.
4) dispense
Sea cucumber peptide composite nutrition powder is pressed into 10g/ bags, pouch sealing.
Embodiment 3
Formula composition：
The raw material of composite plant ferment powder is jujube, mulberries, wherein fig, 45 parts of jujube, 30 parts of mulberries, fig 60
Part.
The preparation technology of sea cucumber peptide composite nutrition powder is：
1) preparation of sea cucumber Gly-His-Lys
Sea cucumber is soaked, cleans the water for removing 2 times of silt, crushings plus sea cucumber weight, add 6wt% compound protease with
4wt% compound fertilizer productions, 50 DEG C of enzymolysis 6h, high-temperature boiling, obtain sea cucumber hydrolysate；With the weight of 2 times of sea cucumber hydrolysate, dense
Spend the ethanol for 80%, temperature is at 10 DEG C, time 16h, alcohol precipitation；Concentration, spray drying, obtains sea cucumber Gly-His-Lys.
4) preparation of composite plant ferment powder
Take jujube, mulberries, fig to mix in proportion, be homogenized；Entered from 1wt% zytases and 3wt% Novi letter enzyme
Row double enzymolysis, pH 6.5,55 DEG C of temperature, enzymolysis time 4h；It is 20wt% that enzymolysis liquid is concentrated into soluble solid content,
Obtain composite plant concentration enzymolysis liquid；After sterilizing, 0.3wt% saccharomycete is inoculated with, in the case where 35 DEG C ensure abundant dissolved oxygen, is carried out
Saccharomycetes to make fermentation 16h；1.5wt% lactic acid bacterias are inoculated with, in 40 DEG C of standings, carry out lactobacillus-fermented 18h；Spray drying, is obtained compound
Plant enzyme powder.
3) dispensing
By sea cucumber Gly-His-Lys and composite plant ferment powder, by weight percentage for：Sea cucumber peptide Fen ﹕ composite plants ferment powder=
65 ﹕ 45, it is well mixed, obtains sea cucumber peptide composite nutrition powder.
4) dispense
Sea cucumber peptide composite nutrition powder is pressed into 10g/ bags, pouch sealing.
Test example
1) the depilation anesthesia of small white mouse：
After first small white mouse depilation is handled, a small amount of absorbent cotton is put into 250ml triangular flasks, pours into 10ml ether, left hand band
Rubber gloves are clamped mouse tail root with little finger of toe with the third finger, and middle finger, forefinger and thumb are by mouse skin of neck together with two
Ear is picked up together, and mouse head then is filled in into triangular flask, puts in about 3cm, and head can not be attached in triangle bottle wall,
Manual time-keeping, survey optimal recovery time.
2) the elongated wound manufacture of small white mouse：
The plastics strip for making a diameter of 0.5cm by oneself is caught into color, mouse back is imprinted on and is positioned, existed with knife blade
The standardized elongated wound of mouse back, as deep as subdermal muscle.
3) manufacture of circular wound
The circular plastic collar for making a diameter of 0.5cm by oneself is caught into color, mouse back is imprinted on and is positioned, with aseptic nipper from
Circle center picks up mouse skin, then wipes out skin of back along trace with sterile scissors, and it is basic just to obtain size, the depth
Consistent circular wound.
4) grouping experiment：
The small white mouse for taking 40 body weight to be 20 ± 2 grams, is randomly divided into 4 groups, manufactures a diameter of 0.5cm circular wound.The
One group is used running water gavage 0.5ml, i.e. control group daily；Second group is added with the sea cucumber peptide composite nutrition powder 1g of embodiment 1 daily
Water 0.5ml brews gavage after 10min, that is, tests A groups；3rd group adds water 0.5ml to brew with the sea cucumber Gly-His-Lys 1g of embodiment 1 daily
Gavage after 10min, that is, test B groups；4th group adds water 0.5ml to brew 10min with the composite plant ferment powder 1g of embodiment 1 daily
Gavage afterwards, that is, test C groups.
5) wound healing is observed：
Visually observe：
Wound size every other day is measured with set square, and observes wound situation of change.
Wound healing is observed under microscope：
Main detection angiogenesis distribution situation and granulation tissue growing state under microscope.Small white mouse after surgery 1,
3rd, 5,7,11,13 natural gift 6 times, each each group take 1 to make anaesthetic treatment, then pick up circular wound peripheral skin with tweezers, use
Scissors, which is cut to be placed on, scribbles 1：On the slide of 1 glycerine protein adhesive (the inside is upward), micro- Microscopic observation is directly put.
6) result is with discussing：
Anaesthetic effect of the ether to small white mouse：
By experiment as can be seen that anesthesia duration 60s effect is preferable, both without dead mouse, recovery time is also more
Properly.
7) wound healing visual results：
As a result it has been observed that the sea cucumber peptide composite nutrition powder of gavage embodiment 1 must test the wound of A group mouse, than B group, C groups
It can be healed in advance with the mouse wound of control group, after this group of wound healing, the smooth no incrustation in surface, and B groups, C groups and control
There is incrustation on surface after group wound healing, it is taken as that sea cucumber peptide composite nutrition powder is to promoting wound healing to have remarkable result.
Table 1 is wound healing visual results.
Table 1
| Averagely fully recover number of days (my god) | Wound healing situation | |
| A groups | 3 | Without incrustation |
| B groups | 6 | There is incrustation |
| C groups | 8 | There is incrustation |
| Control group | 13 | There is incrustation |
Claims (10)
1. a kind of sea cucumber peptide composite nutrition powder, it is characterised in that including sea cucumber Gly-His-Lys and composite plant ferment powder；Described is compound
Plant enzyme powder, raw material include jujube, mulberries, fig.
2. sea cucumber peptide composite nutrition powder according to claim 1, it is characterised in that in the composite plant ferment powder raw material
Jujube, mulberries, the ratio of weight and number of fig are：The Hong Zao ﹕ Sang Shen ﹕ fig=20~﹕ 25~60 of 45 ﹕ 20~30.
3. the preparation technology of the sea cucumber peptide composite nutrition powder described in a kind of claim 1, it is characterised in that step includes：
1) preparation of sea cucumber Gly-His-Lys
Sea cucumber preprocessing, sea cucumber enzymolysis, alcohol precipitation, concentration, spray drying, obtain sea cucumber Gly-His-Lys；
2) preparation of composite plant ferment powder
Jujube, mulberries, fig mixing, homogenate, composite plant enzymolysis, ferment, spray drying, obtain composite plant ferment powder；
3) allocate
Sea cucumber Gly-His-Lys are well mixed with composite plant ferment powder.
4. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 3, it is characterised in that in step 1), sea cucumber
The step of enzymolysis is 3~6wt% compound protease and 2~4wt% compound fertilizer productions, 40~50 DEG C of temperature, during enzymolysis
Between 4~6h, obtain sea cucumber hydrolysate.
5. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 3, it is characterised in that in step 1), alcohol precipitation
The step of be 70~80% with weight, the concentration of 1~2 times of sea cucumber hydrolysate ethanol, temperature at 2~10 DEG C, the time 8~
16h, separating to precipitate.
6. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 3, it is characterised in that compound in step 2)
The step of plant enzymolysis is 0.5~1% zytase and 1~3% Novi believes enzyme, and pH is 5.5~6.5, and temperature is 45~55 DEG C,
Enzymolysis time is 2~4h, and it is 20wt% to be concentrated to soluble solid content, obtains composite plant concentration enzymolysis liquid.
7. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 3, it is characterised in that in step 2), fermentation
The step of for sterilizing；Saccharomycetes to make fermentation；Lactobacillus-fermented.
8. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 7, it is characterised in that the step of saccharomycetes to make fermentation
Rapid is inoculation 0.1~0.3wt% saccharomycete, in the case of ensureing abundant dissolved oxygen, 30~35 DEG C of 12~16h of fermentation.
9. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 7, it is characterised in that the step of lactobacillus-fermented
Rapid is to be inoculated with 0.5~1.5wt% lactic acid bacterias, 37~40 DEG C of 12~18h of fermentation.
10. the preparation technology of sea cucumber peptide composite nutrition powder according to claim 3, it is characterised in that in step 3), sea cucumber
Gly-His-Lys are with composite plant ferment powder percentage by weight：The ﹕ 35~45 of sea cucumber peptide Fen ﹕ composite plants ferment powder=55~65.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112690387A (en) * | 2020-12-24 | 2021-04-23 | 大连鑫澜生物技术有限公司 | Sea cucumber intestine extract probiotic drink capable of strengthening kidney and strengthening body |
| CN113412935A (en) * | 2021-06-29 | 2021-09-21 | 北海海盛食品科技有限公司 | Sea cucumber beauty health product and preparation method thereof |
| CN114522183A (en) * | 2022-01-24 | 2022-05-24 | 江南大学 | Sea cucumber extract for increasing bone mineral density |
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Application publication date: 20180109 |