CN103431467A - Asparagus extract bifidobacterium fermentation activity beverage and preparation method thereof - Google Patents
Asparagus extract bifidobacterium fermentation activity beverage and preparation method thereof Download PDFInfo
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- CN103431467A CN103431467A CN2013103312138A CN201310331213A CN103431467A CN 103431467 A CN103431467 A CN 103431467A CN 2013103312138 A CN2013103312138 A CN 2013103312138A CN 201310331213 A CN201310331213 A CN 201310331213A CN 103431467 A CN103431467 A CN 103431467A
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Abstract
The invention relates to an asparagus extract bifidobacterium fermentation activity beverage and a preparation method thereof. An asparagus extract, brown sugar and stachyose serve as a culture medium; the following strains as shown in the specification are subjected to anaerobic fermentation to culture a bifidobacterium; after the anaerobic fermentation, a fermented probiotic liquid is subjected to sterile filling; and the bifidobacterium, lactobacillus plantarum, lactobacillus acidophilus and saccharomyces cerevisiae are freeze-dried bacteria bought from a culture collection center. The beverage supplements a specific bifidus factor and an exogenous bifidus factor in a plant in order to culture high-density bifidobacterium longum, increase the unit bacterial count of other lactic acid bacteria, conduct enzymolysis of small protein converting peptide in the culture medium, increase the content of amino acid essential for a human body, and improve the activity of asparagus juice, and a health care function of the whole fermentation liquid. The technology has the advantages that a strict anaerobic condition of the bifidobacterium is overcome, an expensive bifidobacterium fermentation device is removed, and the health care function of metabolic products of an active substance and a microorganism containing asparagus, as well as the fermentation liquid of the viable bacteria is enhanced.
Description
Technical field: the preparation method who the present invention relates to the two qi probiotics fermention active beverages of a kind of Germinatus Phragmitis extract.
Background technology
Probiotic beverage, be most favored by customers on market, has the supping of abundant nutrition and medicinal function.Yet, supply at present the probiotic beverage in market, functional not strong, the use of the additives such as artificial color and seasoning essence, thickener, anticorrisive agent, more make the beverage of existing listing, exist many unhealthful unsafe factors and the single most of milk class beverage of struggling, it is also deactivation that fermented beverage is arranged, and really has the viable bacteria beverage of health care seldom.The deep level development utilization that the present invention carries out with regard to health-care effect and the function of beverage.
Asparagus English name: Cacumen Asparagi (drawing); Asparagus definition: the tender stem of liliaceous plant asparagus Asparagus officinal is L..Application branch of learning traditional Chinese medicine and pharmacy (one-level subject); Chinese pharmacology (secondary subject); Chinese medicine (secondary subject), asparagus is one of the world's ten large famous dishes, has another name called asparagus.Enjoy in the international market the laudatory title of " kings of vegetables ", asparagus is rich in several amino acids, protein and vitamin, its content is all higher than general fruits and vegetables, the particularly asparagine in asparagus and trace elements of selenium, molybdenum, chromium, manganese etc., there is the adjusting organism metabolism, improve the effect of body immunity, in the prevention to hypertension, heart disease, leukaemia, leukemia, oedema, cystitis etc. and treatment, there is very strong inhibitory action and pharmacodynamics effect.Stachyose English name: stachyose; Lupeose definition: " the non-reduced tetrose formed by galactolipin, glucose and fructose.Its structural formula is: D-galactopyranose base α-1
Application branch of learning: 6-D-glycopyranosyl α-1 (one-level subject); 2-beta-D-fructofuranose glycosides, belong to the derivative products of sucrose, is the homologue of gossypose, on the hydroxyl of the galactolipin C-6 position of gossypose with α-1 (secondary subject); The 6-glycosidic bond connects a galactolipin again, is distributed widely in plant." (secondary subject) stachyose is naturally occurring a kind of tetrose, is a kind of functional oligose that can significantly promote the beneficial bacterium propagation such as Bifidobacterium.Sterling is white powder, distinguishes the flavor of slightly sweet, and sugariness is sucrose 22%, and good taste, without any bad mouthfeel or peculiar smell.Its molecular structure: " galactolipin-galactolipin-glucose-fructose ", molecular formula is C24H42021, relative molecular weight 666.59.
Stachyose has extremely significantly proliferation function to profitable strains such as the Bifidobacterium in human gastrointestinal tract, Bacillus acidi lacticis, can improve rapidly environment in human body alimentary canal, regulates micro-ecological bacterial group balance.It can promote to form the dominant bacteria status of beneficial bacterium in alimentary canal, suppress the production that aerogenesis produces the spoilage organisms such as sour clostridium, produce in addition a large amount of physiological activators, regulating intestinal canal pH value, kill pathogenic bacteria, checking spoilage product generates, suppress endogenous carcinogenic generation and absorption, and decomposition derives the multiple immunologic function factor.Be commonly called as: the food of beneficial bacterium.
1. stachyose is similar to some water-soluble dietary fiber, nontoxic, direct-edible.
2. stachyose has extremely significantly proliferation function to profitable strains such as the Bifidobacterium in human gastrointestinal tract, Bacillus acidi lacticis, can improve rapidly environment in human body alimentary canal.
3. stachyose is not destroyed in the organs such as the stomach of human body, small intestine, can directly arrive the large intestine position at intestinal beneficial bacterium group place, significantly promotes Bifidobacterium propagation.
4. stachyose can promote the synthetic of the vitamins such as B1, B2, B6, B12, promotes intestines and stomach to calcium, the absorption of the elements such as magnesium.
5. stachyose is not the material of intestinal tympanites effect, does not produce abdominal distension after taking, and quietly moistens intestines and stomach.
6. stachyose has active factors, adsorbable intestines and stomach noxious material and pathogen, and then strengthen body immunity.
Summary of the invention:
The object of the invention is to provide a kind of two qi probiotics fermention active beverages of Germinatus Phragmitis extract that increase the amino acid content of needed by human.The present invention is able to a purpose and is to provide the two qi probiotics fermention active bacterial beverage preparation methods of a kind of Germinatus Phragmitis extract: the symphysis metamember perfect adaptation that probio and medicinal asparagus are contained has also been carried out the probiotics fermention experiment, strengthens the human body disease-proof functions.
Technical scheme of the present invention is the two qi probiotics fermention active bacterial beverages of a kind of Germinatus Phragmitis extract, adopt Germinatus Phragmitis extract, brown sugar, stachyose, as culture medium, is used following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Have a liking for yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Plant lactobacillus bacterium number: CCCi0141, Lactobacillusplantarum (Orla-Jehsen) Holland; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation is produced carries out sterile filling, described Bifidobacterium, plant lactobacillus, have a liking for the yogurt bacterium, have a liking for the yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum of buying from DSMZ, each bacterial classification mixing quality percentage is: 25%, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1; Described Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, Germinatus Phragmitis extract is the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is buying from market of 90% content, Germinatus Phragmitis extract: the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
The two qi probiotics fermention active bacterial beverage preparation methods' of Germinatus Phragmitis extract of the present invention technical scheme is: A: fermented asparagus juice
the preparation method;
1, prepare asparagus juice and Oilgosaccharkdes and other auxiliary materials
The asparagus juice got ready and Oilgosaccharkdes and other auxiliary materials are added to fermentation tank, with sodium carbonate, regulate acid-base value, make the pH value reach 6.4;
2, raw and auxiliary material is carried out to heat sterilization, 121 ℃ of temperature, sterilizing half an hour, and be cooled to rapidly 40 degree;
3, connect bacterium by sterile working
40 ℃ of fermentations of heat-preservation fermentation 30 hours, PH4-4.5, lactic acid 0.85-1%, finish fermentation;
4, product index yellowish-brown or light yellow muddy state, have delicate fragrance and the lactic acid fermented fragrance of asparagus, sour and sweet palatability, pH value 4-4.5, lactic acid percentage composition 0.85-1%, lactic acid bacteria 10
8/ ml soluble solid " 3%, contain vitamin C 14.52mg calcium 41-44mg in every 100ml fermenation raw liquid;
5, detect without pathogenic bacteria, Escherichia coli quantity is lower than national standard, and it is qualified to be considered as;
6 by the stoste fermented, and carries out sterile filling;
7 finished product packing warehouse-ins;
B: get steps A prepared finished product Germinatus Phragmitis extract stoste and brown sugar, stachyose is by stoste 10%, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into the fermentation tank sterilizing and finishes in 1 hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: 25%: 25%: 25%: 25%, 1: 1: 1: 1, the consumption of mixed culture fermentation agent: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
As shown in Figure 1, amino acid whose content in the asparagus juice without fermentation and fermentation is detected, except methionine, have the content of 6 seed amino acids all to increase to some extent, increase altogether 46.9%, nutriment increases considerably.
Be the amino acid whose content of flavor in asparagus juice without fermentation and fermentation as shown in Figure 2, detected, glutamic acid increases by 17%, and asparatate increases by 32%, so be that mouthfeel and taste all change to some extent.
The present invention has the following advantages:
1, principle of the present invention; To use without the antagonistic microbe intergrowth theory, the aerobic bacteria air is taken oxygen by force, for the strict anaerobic bacteria of condition is created good growing environment, in supplementary plant, distinctive Oilgosaccharkdes and foreign aid's Oilgosaccharkdes are to be to cultivate the high density bifidobacterium longum, also allow other lactic acid bacteria improve unit bacterium number, the albumen in the enzymolysis culture medium transforms little peptide, increases the amino acid content of needed by human, improve the activity of asparagus juice, and the health care of whole zymotic fluid.
2 technological advantages overcome the strict anaerobic condition of Bifidobacterium, make good use of oxygen animalcule, carry out air and take oxygen by force, omitted the installation for fermenting of expensive Bifidobacterium, make the common industrialization production of Bifidobacterium, optimized culture medium and cultivated the bar condition, having made the active material and the metabolite of microorganism and the zymotic fluid health care enhancing of viable bacteria that contain asparagus.
The dry Germinatus Phragmitis extract of 3 use and stachyose Oilgosaccharkdes, as Bifidobacterium, plant lactobacillus, have a liking for the fermentation substrate matter of the probio of the symbiosis such as yogurt bacterium, saccharomycete, further widened and increased the functional of asparagus.
4 use Germinatus Phragmitis extracts and stachyose Oilgosaccharkdes are done culture medium, cultivate Bifidobacterium, plant lactobacillus, have a liking for the probios such as yogurt bacterium, Lactobacillus brevis, saccharomycete, not only increase the health care of asparagus in nutrient solution, under this special fermentation substrate environment, the high-activity probiotics active bacteria formulation of cultivating, can also be the activity and the property of medicine that improve the effective health-care components of merit asparagus.
5 for further probiotics preparation and the fermented beverage of the Chinese medicine asparagus of a kind of novel medicine-food two-purpose of research and development provide the research and development thinking; Utilize the active ingredient of asparagus to provide sufficient nutrition, probio to make again the drug effect of asparagus be improved for the probio growth.
6 raw material of the present invention sources are abundant, with low cost;
7 stable preparation process of the present invention, investment of production equipment is less, simple to operate, just with industrialization;
The present invention, making every effort to explore the existing functional beverage of making of solution, the drawback aspect existed, after use Germinatus Phragmitis extract and stachyose Oilgosaccharkdes are done the fermentation substrate of fermented beverage, the functional enhancing that human body is prevented and cured diseases, be the symphysis metamember perfect adaptation that contains of probio and medicinal asparagus and carried out the probiotics fermention experiment, illustrate that disease-proof functions strengthens, the further deep level development that carries out probiotic fermentation beverage utilizes scope.
Principle of the present invention; To use without the antagonistic microbe intergrowth theory, the aerobic bacteria air is taken oxygen by force, for the strict anaerobic bacteria of condition is created good growing environment, in supplementary plant, distinctive Oilgosaccharkdes and foreign aid's Oilgosaccharkdes are to be to cultivate the high density bifidobacterium longum, also allow other lactic acid bacteria improve unit bacterium number, albumen in the enzymolysis culture medium transforms little peptide, improves the property of medicine of asparagus, and the health care of whole zymotic fluid.
This technological advantage overcomes the strict anaerobic condition of Bifidobacterium, make good use of oxygen animalcule, carry out air and take oxygen by force, omitted the installation for fermenting of expensive Bifidobacterium, make the common industrialization production of Bifidobacterium, optimized culture medium and cultivated the bar condition, having made the active material and the metabolite of microorganism and the zymotic fluid health care enhancing of viable bacteria that contain asparagus.
The accompanying drawing explanation:
Amino acid content (mg/100ml) table that Fig. 1 is needed by human in fermentation and unfermentable asparagus juice
Fig. 2 is the amino acid whose content of flavor (mg/100ml) table for fermentation and unfermentable asparagus juice
Concrete embodiment
The two qi probiotics fermention active bacterial beverages of a kind of Germinatus Phragmitis extract, adopt Germinatus Phragmitis extract, brown sugar, and stachyose, as culture medium, is used following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Have a liking for yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Plant lactobacillus bacterium number: CCC10141, Lactobacillusplantarum (Orla-Jehsen) Holland; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation is produced carries out sterile filling, described Bifidobacterium, plant lactobacillus, have a liking for the yogurt bacterium, have a liking for the yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum of buying from DSMZ, each bacterial classification mixing quality percentage is: 25%, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1; Described Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, Germinatus Phragmitis extract is the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is buying from market of 90% content, Germinatus Phragmitis extract: the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
The two qi probiotics fermention active bacterial beverage preparation methods of Germinatus Phragmitis extract:
A. fermented asparagus juice
the preparation method;
1, prepare asparagus juice and Oilgosaccharkdes and other auxiliary materials
The asparagus juice got ready and Oilgosaccharkdes and other auxiliary materials are added to fermentation tank, with sodium carbonate, regulate acid-base value, make the pH value reach 6.4;
2, raw and auxiliary material is carried out to heat sterilization, 121 ℃ of temperature, sterilizing half an hour, and be cooled to rapidly 40 degree;
3, connect bacterium by sterile working
40 ℃ of fermentations of heat-preservation fermentation 30 hours, PH4-4.5, lactic acid 0.85-1%, finish fermentation;
4, product index yellowish-brown or light yellow muddy state, have delicate fragrance and the lactic acid fermented fragrance of asparagus, sour and sweet palatability, pH value 4-4.5, lactic acid percentage composition 0.85-1%, lactic acid bacteria 10
8/ ml soluble solid " 3%, contain vitamin C 14.52mg calcium 41-44mg in every 100ml fermenation raw liquid;
5, detect without pathogenic bacteria, Escherichia coli quantity is lower than national standard, and it is qualified to be considered as;
6 by the stoste fermented, and carries out sterile filling;
7 finished product packing warehouse-ins;
B: get steps A prepared finished product Germinatus Phragmitis extract stoste and brown sugar, stachyose is by stoste 10%, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into the fermentation tank sterilizing and finishes in 1 hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCCi0141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: 25%: 25%: 25%: 25%, 1: 1: 1: 1, the consumption of mixed culture fermentation agent: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
Embodiment 1
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 10% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into fermentation tank sterilizing end half an hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
Embodiment 2
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 20% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 78% pumps into fermentation tank sterilizing end half an hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
Embodiment 3
Get Germinatus Phragmitis extract stoste, brown sugar, stachyose is stoste 30% by mass percentage, brown sugar 1%, stachyose 1%, the ratio of water 68% pumps into fermentation tank sterilizing end half an hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1, the consumption of leavening: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
Claims (2)
1. two qi probiotics fermention active bacterial beverages of a Germinatus Phragmitis extract, it is characterized in that, adopt Germinatus Phragmitis extract, brown sugar, stachyose, as culture medium, is used following bacterial classification to carry out anaerobic fermentation and cultivates Bifidobacterium: bifidobacterium longum bacterium number: ACCC00037Bifidobacterium; Have a liking for yogurt bacterium bacterium number: ACCC10637Lactobacillus acidophilus; Plant lactobacillus bacterium number: CCC10141, Lactobacillusplantarum (Orla-Jehsen) Hol land; S. cervisiae number: ACCC20042, Saccharomycescerevisiae; After completing anaerobic fermentation, the probio liquid that fermentation is produced carries out sterile filling, described Bifidobacterium, plant lactobacillus, have a liking for the yogurt bacterium, have a liking for the yogurt bacterium, S. cervisiae is the freeze-drying microbial inoculum of buying from DSMZ, each bacterial classification mixing quality percentage is: 25%, each bacterial classification mixing quality percentage is: respectively account for 25%, 1: 1: 1: 1; Described Germinatus Phragmitis extract and stachyose Oilgosaccharkdes, Germinatus Phragmitis extract is the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is buying from market of 90% content, Germinatus Phragmitis extract: the extract that active ingredient is 10: 1, the stachyose Oilgosaccharkdes is mass percent 90% content, and raw material drops into stachyose Oilgosaccharkdes 1%, the water 68%-88% that mass percent is Germinatus Phragmitis extract stoste 10-30%, brown sugar 1%, 90% content.
2. two qi probiotics fermention active bacterial beverage preparation methods of a Germinatus Phragmitis extract is characterized in that:
A: fermented asparagus juice
the preparation method;
(1) prepare asparagus juice and Oilgosaccharkdes and other auxiliary materials
The asparagus juice got ready and Oilgosaccharkdes and other auxiliary materials are added to fermentation tank, with sodium carbonate, regulate acid-base value, make the pH value reach 6.4;
(2) raw and auxiliary material is carried out to heat sterilization, 121 ℃ of temperature, sterilizing half an hour, and be cooled to rapidly 40 degree;
(3) connect bacterium by sterile working
40 ℃ of fermentations of heat-preservation fermentation 30 hours, PH4-4.5, lactic acid 0.85-1%, finish fermentation;
(4) product index yellowish-brown or light yellow muddy state, have delicate fragrance and the lactic acid fermented fragrance of asparagus, sour and sweet palatability, pH value 4-4.5, lactic acid percentage composition 0.85-1%, lactic acid bacteria 10
8/ ml soluble solid " 3%, contain vitamin C 14.52mg calcium 41-44mg in every 100ml fermenation raw liquid;
(5) detect without pathogenic bacteria, Escherichia coli quantity is lower than national standard, and it is qualified to be considered as;
(6), by the stoste fermented, carry out sterile filling;
(7) finished product packing warehouse-in;
B: get steps A prepared finished product Germinatus Phragmitis extract stoste and brown sugar, stachyose is by stoste 10%, brown sugar 1%, stachyose 1%, the ratio of water 88% pumps into the fermentation tank sterilizing and finishes in 1 hour, be down to 35 ℃ when temperature and add the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is the mixed culture fermentation agent hybrid anaerobic fermentation, leavening is that bifidobacterium longum ACCC00037. has a liking for yogurt bacterium ACCC10637, plant lactobacillus ACCC10141, the mixed culture fermentation agent of saccharomyces cerevisiae ACCC20042, each bacterial classification mixing quality percentage is: 25%: 25%: 25%: 25%, 1: 1: 1: 1, the consumption of mixed culture fermentation agent: 1-10 ‰, wherein each bacterial classification content reaches following requirement both for qualified products: Bifidobacterium>=10
7cfu/g, plant lactobacillus>=10
7cfu/g, have a liking for yogurt bacterium>=10
7cfu/g, saccharomycete>=10
7cfu/g, the temperature of fermentation tank is controlled at 35 ℃ of constant temperature, and continuing fermentation 48-72 hour stops fermentation during for 3.5-4 when the pH value, detect the miscellaneous bacteria number, and qualified zymotic fluid is carried out to sterile filling, and package test obtains finished product.
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| CN104489092A (en) * | 2014-12-01 | 2015-04-08 | 于力欣 | Health drink containing grosvener siraitia extract and active bifidobacterium longum and preparation method for health drink containing grosvener siraitia extract and active bifidobacterium longum |
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| CN106036318A (en) * | 2016-06-12 | 2016-10-26 | 中国食品发酵工业研究院 | Asparagus fermented beverage capable of improving intestinal functions and method for preparing asparagus fermented beverage |
| CN107348276A (en) * | 2017-07-24 | 2017-11-17 | 秦皇岛长胜营养健康科技有限公司 | A kind of method for improving concentration asparagus juice bioavilability and asparagus drink and application |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289841A (en) * | 1999-09-23 | 2001-04-04 | 陈江柱 | Health-care Bifidobaterium factor vinegar |
| CN101627784A (en) * | 2009-08-25 | 2010-01-20 | 陕西科技大学 | Preparation method of stachyose yoghourt |
| CN102258081A (en) * | 2011-08-01 | 2011-11-30 | 陕西科技大学 | Synbiotics liquid goat milk beverage and preparation method thereof |
| CN102318676A (en) * | 2011-08-01 | 2012-01-18 | 陕西科技大学 | Solidified synbiotic goat yogurt and preparation method thereof |
| CN102885343A (en) * | 2011-07-19 | 2013-01-23 | 江西食方食坊中药食品有限公司 | Anticancer health asparagus beverage and preparation method thereof |
-
2013
- 2013-07-24 CN CN201310331213.8A patent/CN103431467B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289841A (en) * | 1999-09-23 | 2001-04-04 | 陈江柱 | Health-care Bifidobaterium factor vinegar |
| CN101627784A (en) * | 2009-08-25 | 2010-01-20 | 陕西科技大学 | Preparation method of stachyose yoghourt |
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