CN101254163A - Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product - Google Patents
Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product Download PDFInfo
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- CN101254163A CN101254163A CNA2007100859138A CN200710085913A CN101254163A CN 101254163 A CN101254163 A CN 101254163A CN A2007100859138 A CNA2007100859138 A CN A2007100859138A CN 200710085913 A CN200710085913 A CN 200710085913A CN 101254163 A CN101254163 A CN 101254163A
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Abstract
The invention relates to a cosmetic and health protecting composition comprising microbial fermented products of effective ingredients of Chinese medicinal materials, and a preparation method thereof. The preparation method comprises the following steps of carrying out anaerobic fermentation of powdered materials or water extracts of raw Chinese medicinal materials with cosmetic and health promoting effects at 30-40 DEG C and pH of 6.8 by using prepared probiotic bacteria starter for around 18 hours (water extract) or 15days (powdered materials), filtering, centrifuging, extracting, concentrating, and lyophilizing to obtain microbial fermentation product containing effective ingredients; and mixing the microbial fermentation product with adjuvants to form the cosmetic and health promoting composition. The cosmetic and health promoting composition not only overcomes the shortcomings of the prior related product, including low bioactivity and high liability to cause skin allergy and infection arising from skin flora imbalance; but also can be easily absorbed by human body and quick action.
Description
Technical field:
The present invention is a kind of beautifying health composition and preparation method that contains the Chinese crude drug effective ingredient microorganism product, relates to little ecological beauty treatment field of health care products.
Technical background: along with the raising of people's living standard and the enhancing of self health consciousness, the Chinese medicine cosmetic health product more and more is subjected to consumers in general's favor with its unique medical health care function, has shown powerful competitiveness in contend in market.
As the saying goes: " being three fens poison of medicine ", because the Chinese medicine extract that has has sterilization and bacteriostasis efficacy, consumer is added with in the cosmetics processes of Chinese medicine ingredients in use, bad reflections such as allergy, infection occurred.Trace it to its cause, the microecosystem of human epidermal owing to used the cosmetics that sterilization, bacteriostasis are arranged, makes little disruption of ecological balance of skin surface, and a large amount of pathogenic microorganisms are seized the opportunity and grown breeding, above-mentioned said bad reflection just occurred.The appearance of this phenomenon makes a lot of expert calls want the careful Chinese medicine cosmetics of using, thereby one deck shade has been coverd with in the development of Chinese medicine cosmetics.Simultaneously, the Chinese crude drug effective ingredient biological activity of Ti Quing is low according to a conventional method, is unfavorable for absorption of human body.
Modern medicine study shows, the human body dysbacteriosis, cause endocrine dysfunction, vivotoxin increases and can't in time discharge, thereby has increased the weight of the toxin expelling burden of skin, and the time has been grown the function that just can endanger skin, skin can not eupnea and metabolism, will form chloasma, acne, skin will be dry, lack moisture and oil content, and is intense darkness without light.
Summary of the invention:
The objective of the invention is deficiency at the said goods, a kind of beautifying health composition that contains the Chinese crude drug effective ingredient microorganism product is provided, this product is that the Chinese crude drug effective ingredient is transformed through known probiotics fermention, because microorganism has the ability of very powerful decomposition and inversion material, and can produce abundant secondary metabolite, concoct Chinese medicine by microbial growth metabolism and vital movement, can change the property of medicine by a larger margin than the process of preparing Chinese medicine means of general physical or chemistry, improve curative effect, reduce toxic and side effects, enlarge indication.Make Chinese crude drug effective ingredient have stronger biological activity on the one hand, thereby more help absorption of human body with beauty treatment health care efficacy; On the other hand, also farthest reduce the non-specific physiology toxicity of Chinese crude drug, safeguarded the human body microecological balance.The Chinese crude drug effective ingredient that transforms through probiotics fermention is added in the health and beauty care product, by conditioning for oral administration, outside nursing, effective microecosystem regulation balance, eliminate oxygen-derived free radicals in the body, reach and get rid of cytotoxin, eliminate pigementation, slow down the generation of skin wrinkle, thus make skin tenderer, smooth, whiten.
The preferred bifidobacterium adolescentis of probiotic bacteria, bacillus acidophilus, streptococcus thermophilus, the Lactobacillus bulgaricus that the present invention adopts, have a liking for nine kinds of probiotic bacterias such as citric acid leukonid, Bacillus licheniformis, thermophilic actinomycete, Agrobacterium LQ10, high temperature yeast.
The fungus that the present invention adopts comprises thalline and sporophore such as hedgehog hydnum, Ganoderma, Poria, JINZHENGU, the sporophore of preferred JINZHENGU.
The probiotics fermention culture medium of using among the present invention is made up of (Rhizoma Solani tuber osi+kobold+Red Hill leaf+JINZHENGU) cooking liquor, enzymolysis Flyblow albumen powder, yeast powder, sugar and mineral basically.The culture medium of using among the present invention is different from conventional fermentation culture medium for microbe, is primarily characterized in that except that containing basic carbon source and nitrogenous source and mineral, has also added (Rhizoma Solani tuber osi+kobold+Red Hill leaf+JINZHENGU) cooking liquor of larger specific gravity especially.
What should particularly point out here is, the purpose that adds (Rhizoma Solani tuber osi+kobold+Red Hill leaf+JINZHENGU) cooking liquor in the culture medium mainly is the fungus polysaccharide that stachyose, isoflavone, water soluble dietary fiber is provided and probiotic bacteria played propagation and protective effect for probiotic bacteria culture medium.The known water threose is as special-purpose prebiotics and water soluble dietary fiber, and it adjusts the enteral environment rapidly with 40 times the speed propagation probiotic bacteria based on bacillus bifidus, suppresses to be harmful to bacterium, reduces endotoxic generation; Recover the intestinal function rapidly; two-ways regulation is carried out in constipation and diarrhoea; improved body immunity; slow down aging promotes the generation and the absorption of nutrient substance in the intestinal, dispelling toxicity, detoxicating, the liver protecting; bring high blood pressure down and blood fat; blood sugar regulation reduces antibiotic side effect etc., and nearly all intestinal tract disease and other associated disease are all had obvious treatment and improvement effect.Isoflavone is as phytoestrogen, has biological activitys such as antitumor, antibacterial, blood fat reducing, antioxidation, removing free radical, and the control of syndrome, osteoporosis, cardiovascular disease, senile dementia, carcinoma of prostate, colon cancer has benifit to climacteric.Contained water soluble dietary fiber can stop the absorption of neutral fat cholesterol in the intestinal in the Rhizoma Solani tuber osi leachate, promotes gastrointestinal peristalsis, prevents constipation and delays or suppress the absorption of carbohydrate.And the polysaccharide of thalline such as the red ganoderma of Mycophyta, Poria, hedgehog hydnum, JINZHENGU is known as the effect with antitumor and enhance immunity, these polysaccharide also are class prebioticses simultaneously, can obviously improve the biological activity of human body intestinal canal probiotic bacteria, prolong the time-to-live of probiotic bacteria etc.In a word, as one of main component of probiotic bacteria culture medium and alimentation composition of the present invention, represented a main aspect of the technology of the present invention feature with (Rhizoma Solani tuber osi+kobold+Red Hill leaf+JINZHENGU) cooking liquor, it is composed as follows to be used for typical culture of the present invention:
The ratio of water, Rhizoma Solani tuber osi, kobold, Red Hill leaf, JINZHENGU is 1000ml: 200g: 100g: 100g: 50g.In above-mentioned steaming and decocting filtrate, add enzymolysis Flyblow albumen powder 10g, yeast powder 3g, glucose 7g, sucrose 10g, NaCl2g, NaHCO
30.2g, MgSO
40.5g, K
2HPO
4-3H
2O2g, KH
2PO
43g.
Synergistic and detoxification detoxicating functions that the present invention adopts the probiotic bacteria symbiotic fermentation culture medium that the Chinese crude drug effective ingredient with beauty and health care functions is produced; and utilize fungus polysaccharide that probiotic bacteria is produced propagation and protective effect; to combine through Chinese crude drug effective ingredient and relevant auxiliary materials that probiotic bacteria transforms, form the said beautifying health composition of the present invention.Owing to before the Chinese crude drug effective ingredient that process transforms is more unconverted stronger biological activity has been arranged, toxic and side effects also reduces to greatest extent, make the prepared beautifying health composition of the present invention not only overcome present Related product biological activity low, may cause the skin dysbacteriosis and cause the shortcoming of skin allergy, infection, and easier being absorbed by the body, bring into play the effect of its aesthetic health care rapidly.
Below in conjunction with instantiation technical scheme of the present invention is further described:
One, the fermented extracted of Chinese crude drug effective ingredient
A: with the Radix Angelicae Sinensis crude drug is example
1, it is some to get bright Radix Angelicae Sinensis, cleans with clear water, smashs to pieces or shreds with tissue refiner, stand-by.
2, preparation fluid medium: get Rhizoma Solani tuber osi 200g, JINZHENGU 100g, kobold 100g tissue refiner homogenate, with 100g Red Hill leaf grass 0.5cm segment, above-mentioned substance is mixed the container of packing into, adding water 1000ml boiled 20 minutes, filter, add enzymolysis Flyblow albumen powder 10g, yeast powder 3g, glucose 7g, sucrose 10g, NaCl2g, NaHCO in the filtrate
30.2g, MgSO
40.5g, K
2HPO
4-3H
2O2g, KH
2PO
43g, behind each composition mix homogeneously of culture medium, standby in 121 ℃ of autoclavings 30 minutes.
3, preparation leaven: with selected bifidobacterium adolescentis, the bacillus acidophilus, streptococcus thermophilus, Lactobacillus bulgaricus, have a liking for the citric acid leukonid, Bacillus licheniformis, thermophilic actinomycete, Agrobacterium LQ10, nine kinds of probiotic bacterias such as high temperature yeast are inoculated in the liquid seed culture medium respectively, under 30 ℃-40 ℃ environment, cultivated about 20 hours, the mixed of each strain by 1: 1 is inoculated in the liquid fermentation medium, fermentation is 12 hours about 37 ℃, liquid spawn admixed in wheat bran after the sterilization or the starch dry, or in 40 ℃ of constant temperature dryings, contained humidity 6% is chemically examined qualified back packing.
4, will be heated to about 40 ℃ by the culture medium 2000ml of method A (2) preparation, add the bright Radix Angelicae Sinensis 500g that presses A (1) preparation, add 0.5 times then to the leaven of substrate by A (3) preparation, transferring PH is 6.2, under 39 ℃, and anaerobic fermentation 15 days.Every day the test cultures pH value, when the culture pH value reduced to 5.0, accent adds 0.5N NaOH made pH value be approximately 6.2, the 15 days when pH value is reduced to 3.5-3.8, make the fermentation culture medium temperature be reduced to 32 ℃ by the control circulating water temperature, and keep 2 hours to finish the metabolic conversion of tunning.After fermentation is finished, according to the difference of the probiotic fermenting cultures purposes that obtains with this method, can<1〉centrifugalize culture supernatant, obtain containing the Radix Angelicae Sinensis effective ingredient and be not less than 95% bright Radix Angelicae Sinensis microorganism product.<2〉symbiotically fermented probiotic bacteria of centrifugal collection and lyophilizing obtain containing effective composition greater than 95% bright Radix Angelicae Sinensis microorganism product.
B: with the Radix Angelicae Sinensis water extract is example
1, with the bright Radix Angelicae Sinensis chopping of 200g, puts into 2 liters of containers, add 1600ml water logging bubble after 1.5 hours, decocted 1.5 hours, obtain first part of water extract 500ml of bright Radix Angelicae Sinensis.Adding 1200ml decocting boils and got second part of water extract 400ml in 1 hour in container then, merges twice water extract of bright Radix Angelicae Sinensis, and is evaporated to 300ml.
2,2 liters of culture medium are heated to about 40 ℃, and account for the leaven 16g of 0.8% ratio (W/W) of culture medium total amount in the Biomass weight in wet base in the activatory A of pressing of inoculation of medium (3) preparation, be 6.8 at PH, 39 ℃ of temperature insulation anaerobic fermentations 12 hours down, cell concentration reaches 7.0 * 10 in fermentation culture medium
8More than the cfu/ml, and pH value makes the fermentation culture medium temperature be reduced to about about 32 ℃ by the control circulating water temperature when reducing to 3.5-3.8, continues to keep 2-4 hour, to finish the metabolic conversion of microorganism; Press the Radix Angelicae Sinensis extract of B (1) preparation in 1: 1 ratio adding then, under near physiological status condition (37 ℃, PH is 6.2), continue fermentation 18 hours.After fermentation is finished, collect fermentation culture medium, and the centrifugalize supernatant, and the concentrating under reduced pressure lyophilizing, obtain containing effective composition greater than 95% bright Radix Angelicae Sinensis microorganism product.
Two, the preparation of beautifying health composition
1, the preparation of little ecological eliminating toxin and beautifying the skin oral liquid: each Chinese crude drug that will form oral liquid according to dosage mixes back (200g altogether), makes little ecological eliminating toxin and beautifying the skin oral liquid by the preparation method of above-mentioned A.
2, the preparation of emulsus skin care item: CVP Carbopol ETD2050 18g, the sodium hydroxide 0.1g of bright Radix Angelicae Sinensis microorganism product 2g, melatonin 1g, squalane 5g, vaseline 2g, cera alba 0.5g, anhydrous sorbitol sesquialter fatty acid 0.8g, polyoxyethylene oleyl ether 1.2g, propanedione 5g, ethanol 3g, 1% aqueous solution, an amount of essence and antiseptic, add deionized water to 100g, above-mentioned mixing of materials is adopted the even matter of colloid mill, make the emulsus skin care item.
3, the preparation of paste skin care item: bright Radix Angelicae Sinensis microorganism product 3g, melatonin 1g, stearic acid 15g, hexadecanol 1g, sodium hydroxide 0.7g, propanedione 3g, glycerol 5g, an amount of essence and antiseptic, add deionized water to 100g, adopt the even matter of colloid mill to make the paste skin care item above-mentioned mixing of materials.
4, the preparation of liquid cosmetic: bright Radix Angelicae Sinensis microorganism product 2g, melatonin 1g, polyoxyethylene sorbitan mono laurate fat 1g, ethanol 10g, methyl p-hydroxybenzoate 0.2g, glycerol 5g, 1.3-butanediol 5g, an amount of essence and antiseptic, add deionized water to 100g, after above-mentioned material fully mixed, adopt the even matter of colloid mill to make the emulsus skin care item.
5, the preparation of skin care water: take by weighing the Rhizoma Atractylodis Macrocephalae, the Radix Angelicae Dahuricae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Radix Ampelopsis, Poria, the Radix Paeoniae Alba, each 100g of bright Radix Angelicae Sinensis, method by above-mentioned A prepares little ecological extract supernatant, get this microbial fermentation culture supernatant 300ml, glycerol adding 15g, melatonin 1g, an amount of essence and antiseptic add deionized water to 1000ml, and mix homogeneously gets finished product.
Claims (6)
1. a beautifying health composition that contains the Chinese crude drug effective ingredient microorganism product is characterized in that: add the Chinese crude drug effective ingredient through probiotics fermention in beautifying health composition.
2. require the described beautifying health composition that is added with the Chinese crude drug effective ingredient microorganism product according to right 1, it is characterized in that: said composition comprises astringent, cream, breast, frost of eliminating toxin and beautifying the skin oral liquid for oral administration and external etc.
3. by the described beautifying health composition of right 1 requirement, it is characterized in that: in this beautifying health composition comprises the products such as astringent, cream, breast, frost of eliminating toxin and beautifying the skin oral liquid for oral administration and external, add Chinese crude drug effective ingredient through probiotics fermention.
4. require described beautifying health composition according to right 1, it is characterized in that: the Chinese crude drug effective ingredient microorganism product that adds in this beautifying health composition is that crude drug powder or the water extract that will not change tissue transform and get through probiotics fermention.
5. require described beautifying health composition according to right 1, it is characterized in that: be used for fermented tcm material effective ingredient the preferred bifidobacterium adolescentis of probiotic bacteria, bacillus acidophilus, streptococcus thermophilus, Lactobacillus bulgaricus, have a liking for nine kinds of probiotic bacterias such as citric acid leukonid, Bacillus licheniformis, thermophilic actinomycete, Agrobacterium LQ10, high temperature yeast, the sporophore of the preferred JINZHENGU of fungus.
6. the method for the beautifying health composition that production claim 1-5 limits, this method comprises:
(1) preparation fluid medium: this culture medium comprises the cooking liquor, enzymolysis Flyblow albumen powder, yeast powder, sugar, mineral of Rhizoma Solani tuber osi+Red Hill leaf+kobold+JINZHENGU etc.It is composed as follows to be used for typical culture of the present invention:
The ratio of water, Rhizoma Solani tuber osi, kobold, Red Hill leaf, JINZHENGU is 1000ml: 200g: 100g: 100g: 50g.In above-mentioned steaming and decocting filtrate, add enzymolysis Flyblow albumen powder 10g, yeast powder 3g, glucose 7g, sucrose 10g, NaCl2g, NaHCO
30.2g, MgSO
40.5g, K
2HPO
4-3H
2O2g, KH
2PO
43g.
(2) preparation leaven: each the probiotic bacteria kind that will select is inoculated in the liquid seed culture medium respectively, under 30 ℃-40 ℃ environment, cultivated about 20 hours, the mixed of each strain by 1: 1 is inoculated in the liquid fermentation medium, fermentation is 12 hours about 37 ℃, liquid spawn admixed in wheat bran after the sterilization or the starch dry, or in 40 ℃ of constant temperature dryings, contained humidity 6% is chemically examined qualified back packing.
(3) Chinese medicine is pulverized, mixed by 1: 5, be heated to 100 ℃, kept 10 minutes, be cooled to 37 ℃ then, transfer PH to 6.8, add 0.5 times, ferment with this understanding about 15 days, fermented product is filtered filtrate centrifuging and taking supernatant to the leaven of substrate with culture medium; Or culture medium is heated to about 40 ℃, and account for 0.8% ratio (W/W) of culture medium total amount at the activatory leaven of inoculation of medium in the Biomass weight in wet base, be 6.8 at PH, 39 ℃ of temperature insulation anaerobic fermentations 12 hours down, cell concentration reaches 7.0 * 10 in fermentation culture medium
8More than the cfu/ml, and pH value makes the fermentation culture medium temperature be reduced to about about 32 ℃ by the control circulating water temperature when reducing to 3.5-3.8, continues to keep 2-4 hour, to finish the metabolic conversion of microorganism; The water extract concentrated solution that adds Chinese crude drug then in 1: 1 ratio under near physiological status condition (37 ℃, PH is 6.2), continues fermentation 18 hours.After fermentation is finished, collect fermentation culture medium, and the centrifugalize supernatant, and the concentrating under reduced pressure lyophilizing, obtain containing the microorganism product of effective composition greater than 95% Chinese crude drug effective ingredient.
(4) microbial compounds of Chinese crude drug effective ingredient with preparation adds in the prescription on request, the preparation beautifying health composition.
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