A kind of plant composition fermentation magma and its preparation method and application
Technical field
The present invention relates to cosmetic field, relates generally to a kind of plant composition fermentation magma and preparation method thereof and answer
With.
Background technique
10~20% moisture is typically contained in healthy normal cuticula, so that skin maintains plentiful and high resilience
State.But due to external or immanent cause makes skin water shortage and when there is skin problem, the water lock of skin and make outlet capacity just
It can decline, skin can dry up, is dim mute, lose tension and gloss;The defence capability of skin can be declined simultaneously, it is easier to be occurred
Phenomena such as itch, furfur and flush, the symptom of even more serious appearance chap and shouting pain can if things go on like this accelerate declining for skin
Always.Therefore, the replenishing water and preserving moisture of skin is very important.
Most of moisturizing cosmetics currently on the market are mostly using moisturizer such as glycerol, butanediol, vaseline in skin
Skin surface forms thin film, so that the drain evaporation of moisture is reduced, although moisturizing can be played to a certain extent,
It is the water shortage problem that cannot fundamentally solve skin, can also brings the bad skin sense such as greasy.Many natural plant active components
Due to containing ingredients such as polysaccharide, amino acid, vitamin, polyphenol there is preferable moisturizing to releive, delay senescence and improve skin quality etc.
Effect can help skin to be restored to the health status of water profit elastic force.But the plant extracts prepared by traditional handicraft, by
In the limitation of Extraction solvent, technique and preparation method etc., the effect of making raw material, which plays, to be difficult to reach expected.And fermentation technique
The active nutrients of more small molecule can be extracted from natural plant resource, enhancing effect reduces toxicity, promotes what raw material used
Validity and safety, while being easier to absorb, so fermentation technique is before the application of natural plant composition above has good application
Scape.
But for the raw material of existing fermentation technique also mainly using Chinese medicine or by the plant component of drying and processing, these are former
Material has dried out, although water can additionally be added during the fermentation, its nutritional ingredient compared with fresh and alive plant greatly
It is affected greatly.
Summary of the invention
The deficiency of vacancy and technology for existing market, the present invention provides the plant societys for having effects that moisturizing is releived
Materialization cosmetic and its application in cosmetics, by having effects that the reasonable of fresh and alive foliage filter screening that moisturizing is releived and component
Proportion, extracts wherein effective component using modern fermentation technique, and obtained fermentation magma is added in cosmetics, has enhancing
It the water lock of skin and makes outlet capacity, helps skin to restore water and moisten elastic force, while reinforcing skin defence capability, it is existing to resist skin allergy
As the effect of, have broad application prospects and huge market value.
In a first aspect, the present invention provides a kind of plant composition for having effects that moisturizing and releiving, the plant composition packet
Component containing following parts by weight, butter fruit 30~50, golden yellow chamomile 20~40, honeysuckle flowers 10~20, aloe barbadensis Miller 10
~15, ginseng 5~10, Radix Notoginseng 5~10.
In the present invention, in long-term production research process, deeply excavate has in natural plants and traditional Chinese medicine inventor
Replenishing water and preserving moisture, moisturizing foster skin, sensitive effective type of releiving, are rationally compounded with the side's of sending principle of " monarch " in Chinese medicine.
It is monarch with butter fruit in this prescription, butter fruit is famous one of tropical fruit (tree), and nutritive value is very high,
Grease is widely used in cosmetics and food industry, and galam butter is a kind of superpower moisturizer and emollient, facilitates surface layer skin
The sebum film of skin restores and keeps moisture, keeps it comfortable, soft, it also has anti-inflammatory property, can effectively treat allergy, burn
The skin problems such as wound, fash and eczema also have certain sun-proof function;Using golden yellow chamomile and honeysuckle flowers as minister, golden yellow ocean is sweet
Chrysanthemum is rich in flavones active component, has good Shu Min, maintenance sensitivity skin, reduces thin red capillary, reduces rubescent, adjustment skin
The effects of color is uneven, can quickly pacify rubescent skin, have good soothing effect to the skin easily itched;Honeysuckle flowers, that is, honeysuckle,
Be known as clearing heat and detoxicating good medicine from ancient times, have effects that clearing heat and detoxicating, anti-inflammatory detumescence, blood-cooling itch-relieving, for carbuncle swells furunculosis and
Red and swollen heat pain caused by acne has good dissipation effect, moreover it is possible to alleviate eczema, the rubescent and itch of prickly heat well;It is drawn with library
Rope aloe is assistant, and the aloe polysaccharide and vitamin being rich in have good nutrition to the skin of human body, moisten, whitening effect, together
When can also anti-inflammatory, sterilization, calm and sun-proof and other effects;Ginseng and Radix Notoginseng are to make, and ginseng is rich in ginsenoside, blood can be promoted to follow
The effect of ring increases the nutrition supply of skin, and deep layer moisturizing prevents dry and aging, plays tonifying Qi beauty treatment and promoting blood circulation, together
When can also improve immune function, prevent destructive stimulus of the skin by external environment, effectively treat itch and decortication, make facial flesh
Skin is moist;Radix Notoginseng has good promoting blood circulation, nourishing blood function, can improve microcirculation, anti-oxidation efficacy with super strength, effectively
Improve cutaneous immunisation power and anti-aging.
Above a variety of fresh and alive plants for having effects that moisturizing is releived share, and effect is mutually coordinated, can enhance the lock of skin
It water and makes outlet capacity, helps skin to restore water and moisten elastic force, while reinforcing skin defence capability, resist the function of skin allergic phenomena
The effect of effect, maintenance skin health, while also there is certain beautifying face and moistering lotion effect, have broad application prospects and huge market
Value.
It is preferably, described to have effects that the plant composition that moisturizing is releived is made of the component including following parts by weight,
Butter fruit 40, golden yellow chamomile 25, honeysuckle flowers 15, aloe barbadensis Miller 10, ginseng 5, Radix Notoginseng 5.
It is preferably, described to have effects that the plant composition that moisturizing is releived is made of the component including following parts by weight,
Butter fruit 35, golden yellow chamomile 30, honeysuckle flowers 15, aloe barbadensis Miller 10, ginseng 5, Radix Notoginseng 5.
It is preferably, described to have effects that the plant composition that moisturizing is releived is made of the component including following parts by weight,
Butter fruit 40, golden yellow chamomile 20, honeysuckle flowers 10, aloe barbadensis Miller 15, ginseng 10, Radix Notoginseng 5.
Second aspect, the present invention provide a kind of plant composition for preparing and having effects that moisturizing is releived as described in relation to the first aspect
The method of magma, to achieve the object of the present invention, present invention employs following technical solutions:
Compound bacteria is added in the form of bacterium solution, and the composite bacteria liquid is that the composite bacteria liquid is Lactobacillus delbrueckii Bao Jiali
Subspecies (Lactobacillus delbrueckii subsp.Bulgaricus) and natural saccharomyces cerevisiae
(Saccharomyces Cerevisiae) in proportion 3:1 composition, concentration 106~108Cfu/ml is purchased from Guangdong Province microorganism
Research institute's Culture Collection.
Fermented and cultured, the fermentation that will be obtained are carried out to the initial system being made of compound bacteria, plant composition filtrate and water
Liquid takes supernatant liquor after filtering, sterilizing, centrifugation to get plant composition fermentation magma is arrived.
The above plant composition fermentation magma, it is characterised in that:
The amount ratio of composite bacteria liquid, plant composition filtrate and water is 10~50mL:10~50g:100~500g.
Preferably, in plant composition fermentation magma (I), composite bacteria liquid, plant composition filtrate and water dosage
Than for 15mL:40g:300g.
Preferably, in plant composition fermentation magma (II), composite bacteria liquid, plant composition filtrate and water dosage
Than for 20mL:45g:500g.
Preferably, in plant composition fermentation magma (III), composite bacteria liquid, plant composition filtrate and water dosage
Than for 30mL:50g:500g.
The plant composition fermentation magma uses following preparation method:
1) it stocks up
Butter fruit is removed into pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;By Curacao reed
Luxuriant growth removes epidermis, takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Rear drainage is cleaned up, is mixed and is squeezed the juice with stainless steel juice extractor,
Its filtrate is taken after filtering, and it is spare that plant composition filtrate is made.
Compound concentration is 106~108The composite bacteria liquid of cfu/ml, pH value are adjusted to 6.5~7.5.
2) it ferments
Plant composition filtrate and deionized water are mixed into investment fermentor, open blender stirring, while being warming up to 95
~100 DEG C of sterilizings, keep the temperature 5~10 minutes, are cooled to 25 DEG C, prepare fermentation.
It opens blender and puts into composite bacteria liquid, be sufficiently mixed strain with material, close stirring and start to ferment.
8~12 hours progress aerobic fermentations of clean oxygen are passed first into, oxygen is then shut off and carries out anaerobic fermentation, when fermentation
Between be 72~120 hours, fermentation temperature control at 26~42 DEG C.
3) it purifies
By gained filtering fermentation liquor, filtrate loses strain in 100~120 DEG C of at a temperature of 10~30min of high pressure sterilization
It is living.Ferment filtrate after sterilizing is centrifuged 25~35min under conditions of 3000~8000r/min, abandons precipitating, collects supernatant
Liquid, plant composition fermentation magma as provided by the invention.
The third aspect, the present invention provide a kind of plant composition fermentation magma such as the preparation of second aspect preparation method.
Fourth aspect, the present invention provide it is a kind of provide it is a kind of as described in second aspect plant composition fermentation magma changing
Application in cosmetic, the cosmetics contain the fermentation magma of the plant composition as described in second aspect.
Preferably, the cosmetics include toner, lotion, face cream, facial mask, mildy wash or BB frost in any one or
At least two combination, preferably facial mask.
Currently, plant composition is extracted in a manner of fermenting using with the situation in cosmetics it is actually rare, by plant
Composition fermentation magma enhances skin resistance, sensitivity of releiving, maintain skin health does not have also for promoting moisture content of skin
Report.In the fresh and alive plant material that the present invention selects with skin-moisturizing, skin, it is calm releive, antiallergic anti-inflammatory, dispel it is red antipruritic effective
Type is rationally compounded with the side's of sending principle of " monarch " in Chinese medicine, to play the maximum effect of raw material.Utilize suitable bacterium
Kind is made with modern zymotechnique, optimizes preparation process, the natural safety of product is without side-effects, and reaction condition is mild, can effectively save
About production cost simplifies production stage, saves the production time, keeps raw material the effect of property to greatest extent.The fermentation technique energy
It enough realizes large-scale industrial production, and can control the stability of product quality.
Preferably, the weight percent of plant composition fermentation magma is 5~95% in the facial mask.
Compared with prior art, the invention has the advantages that:
(1) each Chinese medicine is rationally compounded according to " monarch " side of sending principle, can be synergistic, and maximum plays the beauty treatment of raw material
Effect, more comprehensively than single plant effect.
(2) present invention is that can effectively simplify production technology using the advantage of fermentation extraction method, makes reaction process mild
Under the conditions of carry out.Meanwhile ferment extraction method can with feed change component molecules structure, promoted raw material effect, reduce toxicity and
Irritation, the advantages such as optimization color, taste, is a kind of comparatively ideal plant extract new process, is conducive to promote and apply.
(3) present invention is directly extracted using fresh and alive plant, is avoided the dry degradation in storage of plant material, is mentioned
The high yield of active constituent, while this method does not need that raw material is dried, and directly can be carried out extracting, saves work
Skill step, is more advantageous to industrialized production.
(4) plant composition fermentation magma of the invention is used in facial mask to enhance the water lock of skin and makes outlet capacity,
The effect of helping skin to restore water and moisten elastic force, while reinforcing skin defence capability, resisting skin allergic phenomena, maintains skin health
The effect of, while also there is certain beautifying face and moistering lotion effect.
Specific embodiment
Further to illustrate technological means and its effect adopted by the present invention, below in conjunction with preferred implementation of the invention
Example to further illustrate the technical scheme of the present invention, but the present invention is not limited in scope of embodiments.
1 plant composition of embodiment ferments magma (I) and preparation method thereof
A kind of plant composition for having effects that moisturizing and releiving is made of the component for including following parts by weight:Butter fruit
40, golden yellow chamomile 25, honeysuckle flowers 15, aloe barbadensis Miller 10, ginseng 5, Radix Notoginseng 5;It is multiple in the plant composition fermentation magma
The amount ratio of combined bacteria liquid, plant composition filtrate and water is 15mL:40g:300g.
Preparation method is as follows:
1) it stocks up
Butter fruit is removed into pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;By Curacao reed
Luxuriant growth removes epidermis, takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Rear drainage is cleaned up, is mixed and is squeezed the juice with stainless steel juice extractor,
Its filtrate is taken after filtering, and it is spare that plant composition filtrate is made.
Compound concentration is 106~108The composite bacteria liquid of cfu/ml, pH value are adjusted to 6.5~7.5.
2) it ferments
Plant composition filtrate and deionized water are mixed into investment fermentor, open blender stirring, while being warming up to 100
DEG C sterilizing, keeps the temperature 10 minutes, is cooled to 25 DEG C, prepare fermentation.
It opens blender and puts into composite bacteria liquid, be sufficiently mixed strain with material, close stirring and start to ferment.
10 hours progress aerobic fermentations of clean oxygen are passed first into, oxygen is then shut off and carries out anaerobic fermentation, fermentation time
It is 96 hours, fermentation temperature is controlled at 30~32 DEG C.
3) it purifies
By gained filtering fermentation liquor, filtrate inactivates strain in 120 DEG C of at a temperature of high pressure sterilization 30min.After sterilizing
Ferment filtrate be centrifuged 30min under conditions of 3000r/min, abandon precipitating, collect supernatant, plant as provided by the invention
Composition fermentation magma.
2 plant composition of embodiment ferments magma (II) and preparation method thereof
A kind of plant composition for having effects that moisturizing and releiving is made of the component for including following parts by weight:Butter fruit
35, golden yellow chamomile 30, honeysuckle flowers 15, aloe barbadensis Miller 10, ginseng 5, Radix Notoginseng 5;It is multiple in the plant composition fermentation magma
The amount ratio of combined bacteria liquid, plant composition filtrate and water is 20mL:45g:500g.
Preparation method is as follows:
1) it stocks up
Butter fruit is removed into pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;By Curacao reed
Luxuriant growth removes epidermis, takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Rear drainage is cleaned up, is mixed and is squeezed the juice with stainless steel juice extractor,
Its filtrate is taken after filtering, and it is spare that plant composition filtrate is made.
Compound concentration is 106~108The composite bacteria liquid of cfu/ml, pH value are adjusted to 6.5~7.5.
2) it ferments
Plant composition filtrate and deionized water are mixed into investment fermentor, open blender stirring, while being warming up to 100
DEG C sterilizing, keeps the temperature 10 minutes, is cooled to 25 DEG C, prepare fermentation.
It opens blender and puts into composite bacteria liquid, be sufficiently mixed strain with material, close stirring and start to ferment.
12 hours progress aerobic fermentations of clean oxygen are passed first into, oxygen is then shut off and carries out anaerobic fermentation, fermentation time
It is 72 hours, fermentation temperature is controlled at 35~37 DEG C.
3) it purifies
By gained filtering fermentation liquor, filtrate inactivates strain in 120 DEG C of at a temperature of high pressure sterilization 30min.After sterilizing
Ferment filtrate be centrifuged 30min under conditions of 3000r/min, abandon precipitating, collect supernatant, plant as provided by the invention
Composition fermentation magma.
3 plant composition of embodiment ferments magma (III) and preparation method thereof
A kind of plant composition for having effects that moisturizing and releiving is made of the component for including following parts by weight:Butter fruit
40, golden yellow chamomile 20, honeysuckle flowers 10, aloe barbadensis Miller 15, ginseng 10, Radix Notoginseng 5;It is multiple in the plant composition fermentation magma
The amount ratio of combined bacteria liquid, plant composition filtrate and water is 30mL:50g:500g.
Preparation method is as follows:
1) it stocks up
Butter fruit is removed into pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;By Curacao reed
Luxuriant growth removes epidermis, takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Rear drainage is cleaned up, is mixed and is squeezed the juice with stainless steel juice extractor,
Its filtrate is taken after filtering, and it is spare that plant composition filtrate is made.
Compound concentration is 106~108The composite bacteria liquid of cfu/ml, pH value are adjusted to 6.5~7.5.
2) it ferments
Plant composition filtrate and deionized water are mixed into investment fermentor, open blender stirring, while being warming up to 100
DEG C sterilizing, keeps the temperature 10 minutes, is cooled to 25 DEG C, prepare fermentation.
It opens blender and puts into composite bacteria liquid, be sufficiently mixed strain with material, close stirring and start to ferment.
8 hours progress aerobic fermentations of clean oxygen are passed first into, oxygen is then shut off and carries out anaerobic fermentation, fermentation time is
120 hours, fermentation temperature was controlled at 26~28 DEG C.
3) it purifies
By gained filtering fermentation liquor, filtrate inactivates strain in 120 DEG C of at a temperature of high pressure sterilization 30min.After sterilizing
Ferment filtrate be centrifuged 30min under conditions of 3000r/min, abandon precipitating, collect supernatant, plant as provided by the invention
Composition fermentation magma.
4 plant composition of embodiment fermentation magma moisture saver mask
A kind of plant composition fermentation magma moisture saver mask is shown in Table 1.
Table 1
Component |
Parts by weight |
Deionized water |
To 100 |
Glycerol |
10.00 |
Butanediol |
7.00 |
Sodium Hyaluronate |
0.10 |
Xanthan gum |
0.10 |
Hydroxyethyl cellulose |
0.10 |
Trehalose |
2.00 |
Glycine betaine |
1.00 |
1,2- hexylene glycol |
0.80 |
Parahydroxyacet-ophenone |
0.50 |
Plant composition ferments magma (I) |
30.00 |
Preparation method is as follows:
(1) xanthan gum and Sodium Hyaluronate are dispersed in advance in glycerol, it is then that trehalose, glycine betaine and ethoxy is fine
Dimension element is put into deionized water together, is heated to 80 DEG C, and stirring and dissolving to homogeneous transparent cools down;
(2) parahydroxyacet-ophenone is dissolved by heating in advance to transparent in butanediol and 1,2- hexylene glycol, is cooled down in system
Investment stirring and dissolving is uniform when to 55 DEG C;
(3) it when being cooled to 40 DEG C, by plant composition fermentation magma (I) investment, is evenly stirred until and is completely dissolved;
(4) color, pH, fragrance, the physical and chemical indexes such as appearance, after the assay was approved 200 mesh filtering and discharging are examined.
5 plant composition of embodiment fermentation magma is releived facial mask
A kind of plant composition fermentation magma facial mask of releiving is shown in Table 2.
Table 2
Component |
Parts by weight |
Plant composition ferments magma (II) |
To 100 |
Glycerol |
7.00 |
Butanediol |
5.00 |
Sodium Hyaluronate |
0.10 |
Xanthan gum |
0.10 |
Hydroxyethyl cellulose |
0.10 |
Dipotassium glycyrrhizinate |
0.10 |
Allantoin |
0.50 |
Panthenol |
2.00 |
1,2- hexylene glycol |
0.80 |
Parahydroxyacet-ophenone |
0.50 |
Preparation method is as follows:
(1) xanthan gum and Sodium Hyaluronate are dispersed in advance in glycerol, then by allantoin, panthenol, dipotassium glycyrrhizinate
It is put into together with hydroxyethyl cellulose in plant composition fermentation magma (II), stirring and dissolving is transparent to being uniformly dispersed;
(2) parahydroxyacet-ophenone is dissolved by heating in butanediol and 1,2- hexylene glycol in advance to transparent, investment system is extremely
Stirring and dissolving is uniform;
(3) color, pH, fragrance, the physical and chemical indexes such as appearance, after the assay was approved 200 mesh filtering and discharging are examined.
6 plant composition of embodiment fermentation magma maintenance sleep mask
A kind of plant composition fermentation magma maintenance sleep mask is shown in Table 3.
Table 3
Component |
Parts by weight |
Deionized water |
To 100 |
Glycerol |
7.00 |
Butanediol |
5.00 |
Sodium Hyaluronate |
0.10 |
Xanthan gum |
0.10 |
Carbomer |
0.40 |
Ammonium acryloyl taurate/VP copolymer |
0.30 |
Aminomethyl propanol |
0.20 |
Ring five dimethyl silicone polymer |
5.00 |
Dimethyl silicone polymer |
0.50 |
Parleam |
2.00 |
1,2- hexylene glycol |
0.80 |
Parahydroxyacet-ophenone |
0.50 |
Plant composition ferments magma (III) |
40.00 |
Preparation method is as follows:
(1) xanthan gum and Sodium Hyaluronate are dispersed in advance in glycerol, then with carbomer, acryloyldimethyl ox sulphur
Sour ammonium/VP copolymer is put into deionized water together, is heated to 80 DEG C of stirring and dissolvings to being uniformly dispersed, is cooled down;
(2) parahydroxyacet-ophenone is dissolved by heating in advance in butanediol and 1,2- hexylene glycol, is cooled to 55 DEG C in system
When investment stirring and dissolving to transparent;
(3) when being cooled to 40 DEG C, aminomethyl is added until being uniformly dispersed completely in homogeneous 5 minutes under homogeneous speed 6000rpm
Propyl alcohol and plant composition ferment magma (III), homogeneous 3 minutes under homogeneous speed 3000rpm;
(4) respectively by Parleam, ring five dimethyl silicone polymer and dimethyl silicone polymer investment system,
Homogeneous is complete to being uniformly dispersed under matter speed 1200rpm
(5) color, pH, fragrance are examined, the physical and chemical indexes such as appearance discharge after the assay was approved.
The single fresh and alive plant fermentation magma of comparative example 1~6
Single fresh and alive plant, butter fruit, golden yellow chamomile, honeysuckle flowers, aloe barbadensis Miller, ginseng and Radix Notoginseng are taken respectively.It will
Butter fruit removes pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;Aloe barbadensis Miller is removed into table
Skin takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Each plant material is cleaned up into rear drainage, uses stainless steel juice extractor respectively
Juicing, takes filtrate that plant composition filtrate is made spare respectively after filtering.Compound concentration is 106~108The compound bacteria of cfu/ml
Liquid, pH value are adjusted to 6.5~7.5.
Each fresh and alive plant filtrate and deionized water are mixed into investment fermentor respectively, blender stirring is opened, heats up simultaneously
It sterilizes to 100 DEG C, keeps the temperature 10 minutes, be cooled to 25 DEG C, prepare fermentation.It opens blender and puts into composite bacteria liquid, make strain and object
Material is sufficiently mixed, and is closed stirring and is started to ferment.The amount ratio of composite bacteria liquid, plant filtrate and water is 30mL:50g:500g.
10 hours progress aerobic fermentations of clean oxygen are passed first into, oxygen is then shut off and carries out anaerobic fermentation, fermentation time
It is 96 hours, fermentation temperature is controlled at 30~32 DEG C.By gained filtering fermentation liquor, at a temperature of high pressure sterilization of the filtrate at 120 DEG C
30min inactivates strain.Ferment filtrate after sterilizing is centrifuged 30min under conditions of 3000r/min, abandons and precipitates, in collection
Clear liquid, as single fresh and alive plant fermentation magma.
7 plant composition extract of comparative example
Butter fruit is removed into pericarp and pulp, takes its fruit stone;Take the petal of golden yellow chamomile and honeysuckle flowers;By Curacao reed
Luxuriant growth removes epidermis, takes its mesophyll;Take the root of ginseng and Radix Notoginseng.Each plant material is cleaned up into rear drainage, is squeezed with stainless steel
The mixing juicing of juice machine, takes its filtrate adjustment pH value for neutrality, plant composition extract is made after filtering.
The single fresh and alive plant fermentation magma moisture saver mask of comparative example 8~13
Compared with Example 4, other than plant composition fermentation magma (I) is changed to comparative example 1~6, other conditions
It is same as Example 4.
14 dried plant composition material of comparative example fermentation magma and preparation method thereof
Compared with Example 1, other than plant composition filtrate is changed to dried plant composition powder, other conditions
It is same as Example 1.
Dried plant composition powder preparation method is:Plant society raw material is cleaned, is dried under the conditions of 50 DEG C, powder
100 mesh are broken to, it is spare that plant composition filtrate is made.
The common moisture saver mask of comparative example 15
Compared with Example 4, other than not adding plant composition fermentation magma (I), other conditions and 4 phase of embodiment
Together.
16 plant composition extract moisture saver mask of comparative example
Compared with Example 4, other than plant composition fermentation magma (I) is changed to comparative example 7, other conditions and reality
It is identical to apply example 4.
17 dried plant composition of comparative example fermentation magma moisture saver mask
Compared with Example 4, in addition to by plant composition fermentation magma (I) be changed to comparative example 14 other than, other conditions with
Embodiment 4 is identical.
1 moistening effect functional evaluation of test example
The preparation of 1.1 samples
Embodiment 4, comparative example 8~13, comparative example 16 and 17 are prepared respectively.
1.2 experimental principle
Moistening effect is tested using universally acknowledgedMethod-capacitance method, skin moisture are lost
TEWL is very important parameter to the defense function of assessment skin, and extensive approval has been obtained in the world.Skin
Defense function is more intact, and the content of moisture content will be higher, and the numerical value that skin moisture is lost TEWL is lower.Moisture of skin tests energy
Whether the defense function of discovery skin has been destroyed in time.
1.3 experiment early-stage preparations
Dry skin crowd 90 are chosen, the age was at 20~45 years old.Any product cannot be used (to change within 15 days before recipient site
Cosmetic or topical drug or health care product for oral administration).Before test, it is desirable that subject clean cleaning facial area and after cleaning 2 hours into
Enter 22 ± 1 DEG C of temperature, sit quietly 30min in the constant temperature and humidity room of humidity 50 ± 5%, and keeps relaxation state.
1.4 experimental method
In isoperibol (22 ± 1 DEG C), relative humidity 50 ± 5%, after 1 hour of resting.By (2.0 ± 0.1) mg/cm2
Before embodiment 4, comparative example 8~13, comparative example 16 and 17 are coated in subject's left hand inner forearm (4cm × 4cm) and the right side by dosage
On the inside of arm (4cm × 4cm), blank control (not applying) while testing.Respectively in 2 hours, 4 hours, 6 small for being painted with sample
When, 8 hours test skin hydrauture state.Evaluation result indicates that evaluation result is shown in Table 4 with hydrauture average value.
4 experimental results change rate (%) of table
It is shown by 4 data of table, what the result of skin hydration degree and TEWL can be apparent contrasts embodiment and comparative example
Effect difference.The plant composition extract moisture saver mask prepared using traditional extraction mode is to the improvement of skin and pays no attention to
Think, but containing plant composition fermentation magma, the moisture saver mask of single fresh and alive plant fermentation magma and the fermentation of dried plant composition
The moisture saver mask of magma promotes the moistening effect of skin.Wherein, plant composition ferments magma than single fresh and alive plant
The moisture-keeping efficacy of object fermentation magma is more preferably significant, illustrates that the various plant materials in the present invention can be mutually synergistic, than single
Raw material for skin hydration degree promotion and reduce skin water loss effect become apparent, by experiments have shown that, other realities
It applies example also and has the effect of and is similar.Test example 2 is releived sensitive effect of stimulation functional evaluation
The preparation of 2.1 samples
Embodiment 4, comparative example 8~13, comparative example 16 and 17,10% lactic acid solution are prepared respectively.
2.2 experiment early-stage preparations
Sensitive skin crowd (tingling sensation adds up >=3 points of persons) 90 is chosen, the age was at 20~45 years old.15 days before recipient site
Any product (cosmetics or topical drug or health care product for oral administration) cannot be used.Before test, it is desirable that subject cleans cleaning face
Portion simultaneously enters 22 ± 1 DEG C of temperature in 2 hours after cleaning, and sit quietly in the constant temperature and humidity room of humidity 50 ± 5% 30min, and keeps
Relaxation state.
2.3 experimental method
Half of face test is carried out by random table in experiment, side is to implement 4, and the other side is comparative example, selects subject's nose
Labial groove position is tested, by 10% lactic acid solution 50ul drop diameter be 0.8cm single layer filter paper on, respectively 3min,
4min and 5min inquire subject's tingling sensation, scored by 4 point-scores (0 point is no tingling sensation, and 1 point is slight shouting pain, 2 points
For moderate shouting pain, 3 points are severe shouting pain), the scoring for recording volunteer stimulates scoring (D0) as the side lactic acid.Test terminates
Afterwards, volunteer need to be by that will use, and a side face uses a kind of product, and another side face uses another product.Forbid intersecting when use
It pollutes and uses product 1 time daily as required, and require being paid a return visit for the 14th day (D14) and the 28th day (D28) for test.Each
The lighting condition at time point should be consistent:Curtain closing, illumination appropriate.Subject is in bed recumbency.The inclined angle of bed exists
Each time point should be all consistent.The position of bed must mark on the ground, to ensure that D14 is consistent with the position of D28
Property.The test of the same volunteer is completed by the same survey crew.2.4 experimental result
5 lactic acid of table stimulates test result change rate unit (%)
Test index |
The stimulation scoring of D14 lactic acid |
The stimulation scoring of D28 lactic acid |
Embodiment 4 |
-29.1 |
-29.8 |
Comparative example 8 |
-18.7 |
-20.2 |
Comparative example 9 |
-3.6 |
-4.2 |
Comparative example 10 |
-5.5 |
-5.7 |
Comparative example 11 |
-2.3 |
-1.9 |
Comparative example 12 |
-7.3 |
-9.1 |
Comparative example 13 |
-1.3 |
-1.3 |
Comparative example 16 |
-3.7 |
-1.2 |
Comparative example 17 |
-22.4 |
-24.6 |
It is shown by 5 data of table, what the result of half face test evaluation of human body can be apparent contrasts embodiment and comparative example
Effect difference.Although embodiment and comparative example has certain effect of releiving, plant composition proferment to skin sensitivity
Slurry is for soothing the skin susceptibility highly significant, hence it is evident that is higher than other each comparative examples and compares with single fresh and alive plant fermentation magma
Excellent effect, illustrate that various plant materials in the present invention can be mutually synergistic, effectively enhance the defense function of skin, repair
Multiple keratoderma reduces lactic acid effect of stimulation, by experiments have shown that, other embodiments also have the effect of similar.
Regulating effect of the 3 plant fermentation magma of test example to mouse skin aquaporin 3
The preparation of 3.1 samples
Prepare embodiment 4 respectively, comparative example 8~13, comparative example 16 and 17, the third of 5%DNCB (2.4- dinitrofluorobenzene)
Ketone solution.
3.2 experimental principle
Aquaporin (AQPS) is a kind of memebrane protein family, is mainly expressed on cell membrane, is transported to the property of can choose
Water or water and small molecule solute (such as glycerol).Numerous studies prove that the change of AQP3 is related with the drying of mouse and application on human skin.AQP3
Adjusting either the reason of skin dehydration, it is also possible to the result of dehydration.Cell migrates changing along with cellular morphology
Become and the movement of water, the potentiality of Protein transport water wherein play a significant role.Lack the keratinocyte migration of the expression of AQP 3
It is slow with wound healing.
3.3 experimental method
7 week old healthy mice 100, half male and half female are taken, experimental animal is divided by weight 18-22g according to table of random number
Blank control group, embodiment group, comparative example group, every group 10.Age of mouse 7 weeks, press (2.0 ± 0.1) mg/cm2Dosage exists sample
The position of back of mice (4cm × 4cm) is smeared, and blank control group applies acetone soln 100ul, embodiment group, comparative example group mouse
The acetone soln 100ul containing 5%DNCB is applied outside back, 1 times a week.Age of mouse 7~11 weeks, blank control group was applied using physiological saline
Back is smeared, comparative example group and embodiment group use comparative example and embodiment to smear respectively, 2 times a day, take back of mice within age of mouse 11 weeks
Skin formaldehyde is fixed.
It is expressed with the mRNA of fluorescence quantitative PCR detection mouse skin AQP3, Western blot method detects mouse skin
The protein expression of AQP3
3.4 experimental result
6 mouse skin AQP3mRNA of table expression and AQP3 protein content
Group |
AQP3mRNA expression/β-action |
AQP3 albumen is averaged relative amount/β-action |
Blank control group |
0.38±0.08 |
0.13±0.04 |
Embodiment 4 |
0.42±0.13 |
0.22±0.12 |
Comparative example 8 |
0.56±0.09 |
0.45±0.07 |
Comparative example 9 |
0.78±0.04 |
0.57±0.08 |
Comparative example 10 |
0.62±0.10 |
0.66±0.11 |
Comparative example 11 |
0.89±0.21 |
0.86±0.03 |
Comparative example 12 |
0.77±0.07 |
0.81±0.15 |
Comparative example 13 |
1.04±0.12 |
1.23±0.25 |
Comparative example 16 |
1.10±0.32 |
1.15±0.22 |
Comparative example 17 |
0.48±0.02 |
0.31±0.09 |
The mouse skin AQP3mRNA expression and AQP3 protein expression that result above discovery is induced through skin lesion are significantly raised,
There is different degrees of reduction after the reparation of embodiment and comparative example sample, but the most obvious with the effect of embodiment 4.More than
Data show that embodiment 4 can significantly improve mouse skin AQP3 albumen and the AQP3mRNA expression of skin lesion induction, promote aqueous
The recovery of metabolic function helps to enhance the water lock of skin and makes outlet capacity, promotes the reparation of skin lesion skin, reinforces the anti-of skin
Imperial ability.Show in the reparation to skin aquaporin, advantageously than traditional extraction method using fermentation extraction method, simultaneously
The various mutual cooperative synergism effects of plant material are significant, hence it is evident that are better than single plant.By experiments have shown that, other embodiments also have
There is similar effect.
4 normal individual trial evaluation experimental of test example
Moisture saver mask prepared by selection embodiment 4, comparative example 15~17 is tried to volunteer respectively as laboratory sample
With evaluation.Choosing the volunteer of 120 stemness sensitivity skin, each 60 people of men and women is randomly divided into 4 groups, every group of 15 males, and 15
Women.4 groups of volunteers use the sample of embodiment 4, comparative example 15~17 respectively.
Subject adheres to using product daily, as follows to the improvement result of skin to product progress sensory evaluation after 4 weeks
It (is counted per capita, number represents the number for more approving the assessment item of product) shown in table 7:
7 normal individual trial evaluation result of table
Above volunteer's sensory evaluation data explanation:There is good guarantor containing plant composition fermentation magma of the invention
Wet and sensitive effect of releiving, and extracted relative to common moisture saver mask, using plant composition prepared by traditional extraction mode
Object moisture saver mask is compared with the moisture saver mask containing dried plant composition fermentation magma, and effect is more obvious.
Therefore, plant composition fermentation magma of the present invention can enhance the water lock of skin and make outlet capacity, help skin extensive
Rehydration moistens elastic force, while reinforcing skin defence capability, resists skin allergic phenomena, strong for can effectively restore skin in cosmetics
Health.
Specific embodiments of the present invention are described in detail above, but it is merely an example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, any couple of present invention carries out equivalent modifications and
Substitution is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and
Modification, all should be contained within the scope of the invention.