CN103932199A - Method utilizing microorganism fermentation to extract effective components from mesona blume - Google Patents

Method utilizing microorganism fermentation to extract effective components from mesona blume Download PDF

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Publication number
CN103932199A
CN103932199A CN201310017902.1A CN201310017902A CN103932199A CN 103932199 A CN103932199 A CN 103932199A CN 201310017902 A CN201310017902 A CN 201310017902A CN 103932199 A CN103932199 A CN 103932199A
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China
Prior art keywords
mesona blume
seed strain
strain liquid
effective components
liquid
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Pending
Application number
CN201310017902.1A
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Chinese (zh)
Inventor
陈书勤
孟宪福
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Individual
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Individual
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Priority to CN201310017902.1A priority Critical patent/CN103932199A/en
Publication of CN103932199A publication Critical patent/CN103932199A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method utilizing microorganism fermentation to extract effective components from mesona blume. At present, the enzyme hydrolysis method and the sodium bicarbonate alkaline solution extraction method are used to extract effective components from mesona blume, but these two methods have the disadvantages of high sodium content during the preparation process and high preparation cost. In this method, a mixed bacterial strain solution of work seeds is used, the mixed bacterial strain solution has a separating effect on the cell walls of mesona blume, thus the effective components in mesona blume is easy to dissolve, and moreover, the insoluble diet fibers in mesona blume is converted to soluble diet fibers by the mixed bacterial strain solution, so the diet fiber content in the conventional mesona blume products is increased, and at the same time the sodium content in the conventional mesona blume products is reduced. The method has the advantages of mild conditions, low cost, and convenient operation. The product produced by the method is benefit for human health, and has a great promotion value.

Description

The method of celestial careless active ingredient is extracted in a kind of microorganism fermentation
Technical field
What the present invention relates to is a kind of method that celestial careless active ingredient is extracted in microorganism fermentation, belongs to microbial fermentation technology field.
Background technology
The processing of the celestial grass of China is all also to adopt traditional buck lixiviate mostly, small part active ingredient is extracted the preparation for the careless cold tea of celestial being and bean jelly, and first extraction still contained a large amount of high-quality dietary fibers (Dietary Fiber afterwards, DF) celestial being of active ingredient grass slag is usually used as ultimate waste material, only as also field of organic fertilizer, or as the culture medium of plantation large edible mushroom, added value is lower, has caused the larger wasting of resources.Simultaneously, the sodium element composition that Chinese generally absorbs by dietary food product is higher, become the direct origin cause of formation of bringing out the modern illnesss occurred frequently such as hypertension, and the potential danger factor that traditional buck extraction process exceeds standard with regard to becoming the sodium element of celestial careless cold tea and bean jelly.
Therefore, adopt bioengineering new and high technology transformation traditional handicraft develop the functional new product of celestial being grass dietary fiber of high added value, be the celestial careless industry in " running business into strong one " Guangxi and even China in the urgent need to.Also be one of auxiliary hygienic measures of effective dietotherapy improving by the high fine dietary food product of scientific and reasonable low sodium low fat " three height " physique popualtion healthy level that China increases day by day.
Summary of the invention
Object of the present invention is just to provide the method for the celestial careless active ingredient of a kind of microorganism fermentation extraction, produces the high fine dietary food product of low sodium.This method celestial careless cell membrane that can effectively break, fully discharges its active ingredient and cell lysis wall composition, makes in celestial grass the soluble dietary fiber of part diminish into soluble dietary fiber and enters extract.Replace traditional alkali extracting method, reduce sodium content in traditional celestial grass product.This method technique is simple, and fermentation condition gentleness is beneficial to energy-conservation.
Foregoing invention object, is achieved through the following technical solutions, and the method for celestial careless active ingredient is extracted in a kind of microorganism fermentation, and key step is:
(1) get commercially available candida tropicalis (Candida tropicalis) and lactobacillus bulgaricus (Lactobacillus bulgaricus), after thawing, be inoculated in respectively and in conventional medium, carry out actication of culture, soak time 20-30 hour, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid in conventional medium with 1: the ratio of 10-20 is carried out the successively bacterial classification quantity amplification of 2-4 time, cultivates as work seed strain liquid under 28-36 DEG C of environment;
(3), with 3-5: the quantity of 5-7 is mixed with hybrid working seed strain liquid than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach hundred million/milliliter of 1-2;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water were by 1: 1: 6-10 consumption mixes, and stirred with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Described candida tropicalis Candida tropicalis and lactobacillus bulgaricus Lactobacillus bulgaricus, be ripe commodity bacterial classification, existing merchandise sales.
Take the present invention of above-mentioned measure, the hybrid working seed strain liquid of preparation has decomposition to the careless cell membrane of celestial being, be conducive to active ingredient stripping, and can improve the content of soluble dietary fiber in extract, reduce the sodium content in the celestial herbvore product of tradition, can produce the celestial grass product of the high fine meals of low sodium.Compared with extracting with traditional buck of the celestial careless active ingredient of current extraction, reaction condition gentleness, is beneficial to energy-conservationly, and new product is conducive to health.
Process conditions gentleness of the present invention, cost is lower, easy and simple to handle, and dissemination is very big.
Detailed description of the invention
Embodiment 1
(1) getting specification is frozen lactic acid bacteria and saccharomycete of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after at room temperature thawing, and soak time 24 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in the conventional medium of 100 milliliters, carry out the successively bacterial classification quantity amplification of 3 times with the ratio of 1: 10, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 3: 7 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 100,000,000/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 6 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Embodiment 2
(1) getting specification is frozen pantoea agglomerans, lactic acid bacteria, saccharomycete and Bifidobacterium of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after thawing, and soak time 30 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in conventional medium, carry out the successively bacterial classification quantity amplification of 4 times with the ratio of 1: 20, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 4: 6 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 200,000,000/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 8 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Embodiment 3
(1) getting specification is frozen pantoea agglomerans, lactic acid bacteria, saccharomycete and Bifidobacterium of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after thawing, and soak time 30 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in conventional medium, carry out the successively bacterial classification quantity amplification of 5 times with the ratio of 1: 15, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 5: 5 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 1.5 hundred million/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 10 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.

Claims (1)

1. a method for celestial careless active ingredient is extracted in microorganism fermentation, it is characterized in that described preparation method's key step is:
(1) get commercially available candida tropicalis (Candida tropicalis) and lactobacillus bulgaricus (Lactobacillus bulgaricus), after thawing, be inoculated in respectively and in conventional medium, carry out actication of culture, soak time 20-30 hour, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid in conventional medium with 1: the ratio of 10-20 is carried out the successively bacterial classification quantity amplification of 2-4 time, cultivates as work seed strain liquid under 28-36 DEG C of environment;
(3), with 3-5: the quantity of 5-7 is mixed with hybrid working seed strain liquid than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach hundred million/milliliter of 1-2;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water were by 1: 1: 6-10 consumption mixes, and stirred with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
CN201310017902.1A 2013-01-18 2013-01-18 Method utilizing microorganism fermentation to extract effective components from mesona blume Pending CN103932199A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310017902.1A CN103932199A (en) 2013-01-18 2013-01-18 Method utilizing microorganism fermentation to extract effective components from mesona blume

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310017902.1A CN103932199A (en) 2013-01-18 2013-01-18 Method utilizing microorganism fermentation to extract effective components from mesona blume

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CN103932199A true CN103932199A (en) 2014-07-23

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105053417A (en) * 2015-09-06 2015-11-18 青海之也科技咨询服务有限公司 Lycium ruthenicum Murr tea drink and preparation method thereof
CN105124070A (en) * 2015-09-06 2015-12-09 青海之也科技咨询服务有限公司 Chinese wolfberry fruit flavored health-care tea and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101254163A (en) * 2007-02-27 2008-09-03 程恒明 Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product
CN101711581A (en) * 2009-11-18 2010-05-26 海南椰国食品有限公司 Sianchau nata
CN102166029A (en) * 2011-05-05 2011-08-31 陈明科 Functional drink of multi-bacterium health-keeping extract of mesona chinensis benth
JP2012005362A (en) * 2010-06-22 2012-01-12 T Hasegawa Co Ltd Heat-resistant new gel material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101254163A (en) * 2007-02-27 2008-09-03 程恒明 Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product
CN101711581A (en) * 2009-11-18 2010-05-26 海南椰国食品有限公司 Sianchau nata
JP2012005362A (en) * 2010-06-22 2012-01-12 T Hasegawa Co Ltd Heat-resistant new gel material
CN102166029A (en) * 2011-05-05 2011-08-31 陈明科 Functional drink of multi-bacterium health-keeping extract of mesona chinensis benth

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
钟艳梅,等: "仙草发酵酒饮料的研制", 《现代食品科技》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105053417A (en) * 2015-09-06 2015-11-18 青海之也科技咨询服务有限公司 Lycium ruthenicum Murr tea drink and preparation method thereof
CN105124070A (en) * 2015-09-06 2015-12-09 青海之也科技咨询服务有限公司 Chinese wolfberry fruit flavored health-care tea and preparation method thereof

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Application publication date: 20140723