CN103932199A - Method utilizing microorganism fermentation to extract effective components from mesona blume - Google Patents
Method utilizing microorganism fermentation to extract effective components from mesona blume Download PDFInfo
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- CN103932199A CN103932199A CN201310017902.1A CN201310017902A CN103932199A CN 103932199 A CN103932199 A CN 103932199A CN 201310017902 A CN201310017902 A CN 201310017902A CN 103932199 A CN103932199 A CN 103932199A
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- mesona blume
- seed strain
- strain liquid
- effective components
- liquid
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 title claims abstract description 14
- 230000004151 fermentation Effects 0.000 title claims abstract description 14
- 244000005700 microbiome Species 0.000 title claims abstract description 7
- 241001646834 Mesona Species 0.000 title abstract 8
- 230000001580 bacterial effect Effects 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 18
- 244000025254 Cannabis sativa Species 0.000 claims description 11
- 239000004480 active ingredient Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 230000009286 beneficial effect Effects 0.000 claims description 7
- 235000012054 meals Nutrition 0.000 claims description 6
- 241000222178 Candida tropicalis Species 0.000 claims description 5
- 230000003213 activating effect Effects 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000010257 thawing Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 4
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract description 9
- 229910052708 sodium Inorganic materials 0.000 abstract description 9
- 239000011734 sodium Substances 0.000 abstract description 9
- 235000005911 diet Nutrition 0.000 abstract description 6
- 230000037213 diet Effects 0.000 abstract 3
- 239000000835 fiber Substances 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract 2
- 230000008901 benefit Effects 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000011435 alkaline solution extraction method Methods 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 abstract 1
- 235000017557 sodium bicarbonate Nutrition 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 8
- 235000013325 dietary fiber Nutrition 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000000605 extraction Methods 0.000 description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 3
- 238000005138 cryopreservation Methods 0.000 description 3
- 230000000378 dietary effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 2
- 241000588912 Pantoea agglomerans Species 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000008274 jelly Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method utilizing microorganism fermentation to extract effective components from mesona blume. At present, the enzyme hydrolysis method and the sodium bicarbonate alkaline solution extraction method are used to extract effective components from mesona blume, but these two methods have the disadvantages of high sodium content during the preparation process and high preparation cost. In this method, a mixed bacterial strain solution of work seeds is used, the mixed bacterial strain solution has a separating effect on the cell walls of mesona blume, thus the effective components in mesona blume is easy to dissolve, and moreover, the insoluble diet fibers in mesona blume is converted to soluble diet fibers by the mixed bacterial strain solution, so the diet fiber content in the conventional mesona blume products is increased, and at the same time the sodium content in the conventional mesona blume products is reduced. The method has the advantages of mild conditions, low cost, and convenient operation. The product produced by the method is benefit for human health, and has a great promotion value.
Description
Technical field
What the present invention relates to is a kind of method that celestial careless active ingredient is extracted in microorganism fermentation, belongs to microbial fermentation technology field.
Background technology
The processing of the celestial grass of China is all also to adopt traditional buck lixiviate mostly, small part active ingredient is extracted the preparation for the careless cold tea of celestial being and bean jelly, and first extraction still contained a large amount of high-quality dietary fibers (Dietary Fiber afterwards, DF) celestial being of active ingredient grass slag is usually used as ultimate waste material, only as also field of organic fertilizer, or as the culture medium of plantation large edible mushroom, added value is lower, has caused the larger wasting of resources.Simultaneously, the sodium element composition that Chinese generally absorbs by dietary food product is higher, become the direct origin cause of formation of bringing out the modern illnesss occurred frequently such as hypertension, and the potential danger factor that traditional buck extraction process exceeds standard with regard to becoming the sodium element of celestial careless cold tea and bean jelly.
Therefore, adopt bioengineering new and high technology transformation traditional handicraft develop the functional new product of celestial being grass dietary fiber of high added value, be the celestial careless industry in " running business into strong one " Guangxi and even China in the urgent need to.Also be one of auxiliary hygienic measures of effective dietotherapy improving by the high fine dietary food product of scientific and reasonable low sodium low fat " three height " physique popualtion healthy level that China increases day by day.
Summary of the invention
Object of the present invention is just to provide the method for the celestial careless active ingredient of a kind of microorganism fermentation extraction, produces the high fine dietary food product of low sodium.This method celestial careless cell membrane that can effectively break, fully discharges its active ingredient and cell lysis wall composition, makes in celestial grass the soluble dietary fiber of part diminish into soluble dietary fiber and enters extract.Replace traditional alkali extracting method, reduce sodium content in traditional celestial grass product.This method technique is simple, and fermentation condition gentleness is beneficial to energy-conservation.
Foregoing invention object, is achieved through the following technical solutions, and the method for celestial careless active ingredient is extracted in a kind of microorganism fermentation, and key step is:
(1) get commercially available candida tropicalis (Candida tropicalis) and lactobacillus bulgaricus (Lactobacillus bulgaricus), after thawing, be inoculated in respectively and in conventional medium, carry out actication of culture, soak time 20-30 hour, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid in conventional medium with 1: the ratio of 10-20 is carried out the successively bacterial classification quantity amplification of 2-4 time, cultivates as work seed strain liquid under 28-36 DEG C of environment;
(3), with 3-5: the quantity of 5-7 is mixed with hybrid working seed strain liquid than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach hundred million/milliliter of 1-2;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water were by 1: 1: 6-10 consumption mixes, and stirred with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Described candida tropicalis Candida tropicalis and lactobacillus bulgaricus Lactobacillus bulgaricus, be ripe commodity bacterial classification, existing merchandise sales.
Take the present invention of above-mentioned measure, the hybrid working seed strain liquid of preparation has decomposition to the careless cell membrane of celestial being, be conducive to active ingredient stripping, and can improve the content of soluble dietary fiber in extract, reduce the sodium content in the celestial herbvore product of tradition, can produce the celestial grass product of the high fine meals of low sodium.Compared with extracting with traditional buck of the celestial careless active ingredient of current extraction, reaction condition gentleness, is beneficial to energy-conservationly, and new product is conducive to health.
Process conditions gentleness of the present invention, cost is lower, easy and simple to handle, and dissemination is very big.
Detailed description of the invention
Embodiment 1
(1) getting specification is frozen lactic acid bacteria and saccharomycete of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after at room temperature thawing, and soak time 24 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in the conventional medium of 100 milliliters, carry out the successively bacterial classification quantity amplification of 3 times with the ratio of 1: 10, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 3: 7 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 100,000,000/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 6 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Embodiment 2
(1) getting specification is frozen pantoea agglomerans, lactic acid bacteria, saccharomycete and Bifidobacterium of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after thawing, and soak time 30 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in conventional medium, carry out the successively bacterial classification quantity amplification of 4 times with the ratio of 1: 20, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 4: 6 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 200,000,000/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 8 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Embodiment 3
(1) getting specification is frozen pantoea agglomerans, lactic acid bacteria, saccharomycete and Bifidobacterium of 5ml bacterial classification cryopreservation tube, is inoculated in respectively in the conventional medium of 10 milliliters and carries out actication of culture after thawing, and soak time 30 hours, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid and in conventional medium, carry out the successively bacterial classification quantity amplification of 5 times with the ratio of 1: 15, under 28-36 DEG C of environment, cultivate as work seed strain liquid;
(3) be mixed with hybrid working seed strain liquid with the quantity of 5: 5 than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach 1.5 hundred million/milliliter;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water mix by 1: 1: 10 consumption, stir with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Claims (1)
1. a method for celestial careless active ingredient is extracted in microorganism fermentation, it is characterized in that described preparation method's key step is:
(1) get commercially available candida tropicalis (Candida tropicalis) and lactobacillus bulgaricus (Lactobacillus bulgaricus), after thawing, be inoculated in respectively and in conventional medium, carry out actication of culture, soak time 20-30 hour, obtains activating bacterium liquid;
(2) get respectively above-mentioned activation bacterium liquid in conventional medium with 1: the ratio of 10-20 is carried out the successively bacterial classification quantity amplification of 2-4 time, cultivates as work seed strain liquid under 28-36 DEG C of environment;
(3), with 3-5: the quantity of 5-7 is mixed with hybrid working seed strain liquid than by above-mentioned work seed strain liquid, make the quantity of beneficial bacterium in hybrid working seed strain liquid reach hundred million/milliliter of 1-2;
(4) by cultured hybrid working seed strain liquid, celestial grass meal and water were by 1: 1: 6-10 consumption mixes, and stirred with the speed of 20r/min;
(5) said mixture is at 28-36 DEG C of environment bottom fermentation 4-6 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310017902.1A CN103932199A (en) | 2013-01-18 | 2013-01-18 | Method utilizing microorganism fermentation to extract effective components from mesona blume |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310017902.1A CN103932199A (en) | 2013-01-18 | 2013-01-18 | Method utilizing microorganism fermentation to extract effective components from mesona blume |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103932199A true CN103932199A (en) | 2014-07-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310017902.1A Pending CN103932199A (en) | 2013-01-18 | 2013-01-18 | Method utilizing microorganism fermentation to extract effective components from mesona blume |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103932199A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053417A (en) * | 2015-09-06 | 2015-11-18 | 青海之也科技咨询服务有限公司 | Lycium ruthenicum Murr tea drink and preparation method thereof |
| CN105124070A (en) * | 2015-09-06 | 2015-12-09 | 青海之也科技咨询服务有限公司 | Chinese wolfberry fruit flavored health-care tea and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254163A (en) * | 2007-02-27 | 2008-09-03 | 程恒明 | Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product |
| CN101711581A (en) * | 2009-11-18 | 2010-05-26 | 海南椰国食品有限公司 | Sianchau nata |
| CN102166029A (en) * | 2011-05-05 | 2011-08-31 | 陈明科 | Functional drink of multi-bacterium health-keeping extract of mesona chinensis benth |
| JP2012005362A (en) * | 2010-06-22 | 2012-01-12 | T Hasegawa Co Ltd | Heat-resistant new gel material |
-
2013
- 2013-01-18 CN CN201310017902.1A patent/CN103932199A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101254163A (en) * | 2007-02-27 | 2008-09-03 | 程恒明 | Beauty treatment health care combination containing traditional Chinese herbs effective ingredient microorganism product |
| CN101711581A (en) * | 2009-11-18 | 2010-05-26 | 海南椰国食品有限公司 | Sianchau nata |
| JP2012005362A (en) * | 2010-06-22 | 2012-01-12 | T Hasegawa Co Ltd | Heat-resistant new gel material |
| CN102166029A (en) * | 2011-05-05 | 2011-08-31 | 陈明科 | Functional drink of multi-bacterium health-keeping extract of mesona chinensis benth |
Non-Patent Citations (1)
| Title |
|---|
| 钟艳梅，等: "仙草发酵酒饮料的研制", 《现代食品科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053417A (en) * | 2015-09-06 | 2015-11-18 | 青海之也科技咨询服务有限公司 | Lycium ruthenicum Murr tea drink and preparation method thereof |
| CN105124070A (en) * | 2015-09-06 | 2015-12-09 | 青海之也科技咨询服务有限公司 | Chinese wolfberry fruit flavored health-care tea and preparation method thereof |
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Application publication date: 20140723 |