CN106666101A - Feed additive prepared from fermented tea residues and application thereof - Google Patents
Feed additive prepared from fermented tea residues and application thereof Download PDFInfo
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- CN106666101A CN106666101A CN201611214808.5A CN201611214808A CN106666101A CN 106666101 A CN106666101 A CN 106666101A CN 201611214808 A CN201611214808 A CN 201611214808A CN 106666101 A CN106666101 A CN 106666101A
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- tea
- tea grounds
- fermentation
- feed additive
- edible
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses a feed additive prepared from fermented tea residues and an application thereof, and belongs to the field of micro-organism fermentation. A co-cultivation of edible and medicinal funguses and phaffia rhodozyma is used to conduct the moderate fermentation of the tea, which can preserve and improve beneficial ingredients in the tea residues as much as possible, degrade not easily used cellulose and other substances, can also increase active ingredients in the edible and medicinal funguses, and improve the utilization value of the tea residues. The prepared products by the method contain polyphenols, polysaccharides, astaxanthin, and other substances and the highest crude protein content is up to 36.12%. The provided products can be applied singly and can also be subjected to product compounding application according to animal variety and growth stage. The product preparation and application methods are simple and easy to implement, and the feed is low in costs and remarkable in effects, and at the same time, conducts the comprehensive utilization of the tea residues, and reduces the environmental pollution.
Description
Technical field
The present invention relates to a kind of feed additive of fermentation tea grounds preparation and its application, belong to field of microbial fermentation.
Background technology
With the continuous development of China's tea comprehensive processing industry, the comprehensive utilizating research of tea grounds increasingly receives publicity.Grind
Study carefully and show, containing abundant nutrient substance in tea grounds, wherein crude protein content more than 17%, crude fiber content more than 16%, tea
The plurality of active ingredients such as polyphenol content 1%-2%, also caffeine, mineral.Therefore, tea grounds be of high nutritive value as one kind,
Beneficial to the non-traditional feed resource for improving poultry quality, will fully develop talents in the development of following animal husbandry.
But, crude fiber content is higher in tea grounds, is unfavorable for absorbing for poultry, only adds on a small quantity in feedstuff,
The fodder application of tea grounds is had a strong impact on.
Edible and medicinal fungi be a class can with the Saprobiont of the materials such as efficient utilization cellulose, lignin, it is simultaneously synthesizing such as
Polysaccharide, triterpeness, adenosine class, alkaloid isoreactivity material, and the various enzyme contents of edible and medicinal fungi secretion are very high, its
Distinctive fragrance can improve the palatability of poultry.There is correlational study to produce edible and medicinal fungi sporophore as substrate by the use of tea grounds,
The comprehensive utilization of tea grounds, but the method production cycle longer (more than 3 months) are realized, labor intensity is larger, while edible medicinal is true
The recycling of bacterium bacteria residue is also a urgent problem.
Therefore, urgent need searching one kind can give full play to edible and medicinal fungi and be had the advantage that, realize tea grounds synthesization, feedstuff
Change the scheme of application.
The content of the invention
The purpose of the present invention is to provide one kind first using edible and medicinal fungi and phaffiafhodozyma (Phaffia
Rhodozyma the method that the tea grounds that) ferments prepares feed additive, is to co-culture to carry out tea grounds appropriateness admittedly using edible and medicinal fungi
State is fermented, and obtains feed additive；The proper fermentation referred to by control time and fermentation temperature, makes to be difficult in tea grounds sharp
With mass degradation, beneficiating ingredient is retained as far as possible, and improve nutritive value.
In one embodiment of the invention, the appropriate solid fermentation is to control fermentation time for 5-15 days, fermentation
Temperature 20-25 DEG C；The edible and medicinal fungi includes Pnoliota adiposa(Fr.) Quel. (Pholiota adiposa), Phellinus igniarius (L. ex Fr.) Quel. (Phellinus
Igniarius), one kind in Ganoderma (Ganoderma Lucidum), Cordyceps (Ophiocordyceps sinensis)
Or it is various.
In one embodiment of the invention, the tea grounds is green tea, black tea, Ramulus et Folium Mussaendae Pubescentis or oolong tea effective component extracting
Residue afterwards.
In one embodiment of the invention, the tea grounds is green tea tea grounds.
One embodiment of the present invention comprises the steps：
1) process of tea grounds：It is standby after tea grounds drying, culture medium is directly used as, or it is used as culture medium after crushing；
2) preparation of nutritional solution：By every gram of tea grounds, addition glucose 20-40mg, Magnesium sulfate heptahydrate 3-9mg, phosphoric acid are weighed
Potassium dihydrogen 6-12mg, vitamin B 1-2mg, prepare the nutritional solution of water content 50-80%；
3) preparation of tea grounds culture medium：Will be standby after nutritional solution high temperature sterilize；
4) bacterium solution is prepared：By cultured edible and medicinal fungi seed liquor and phaffiafhodozyma seed liquor by volume 1:1-
1.5 ratios mix；
5) it is inoculated with：By the 5-10% inoculation bacterium solutions of the weight in wet base quality of tea grounds culture medium；
6) ferment：At 20-25 DEG C, ferment 5-15 days；
7) mixture after fermentation ends is crushed after 70-80 DEG C of drying, you can use as feed additive.
In one embodiment of the invention, the culture medium and cultural method of the edible and medicinal fungi and phaffiafhodozyma
For culture medium commonly used in the art and cultural method.
The application of the feedstuff addition can be used as single fermentation products application, it can also be used to the compounding of multiple product.
Beneficial effect：The present invention co-cultures appropriate solid fermentation tea grounds by edible and medicinal fungi and phaffiafhodozyma, relatively
Only with edible and medicinal fungi fermentation, the present invention also improves its nutritive value while benefit materials in being sufficiently reserved tea grounds.Produce
Higher than the situation only with edible and medicinal fungi fermentation, crude protein content is then obtained the content of the material such as polyphenol, polysaccharide, astaxanthin in product
Significant raising has been arrived, 36.12% has been reached as high as.Astaxanthin in the feedstuff that the present invention is provided is that have very in aquaculture
Important feed additive, the resistances against diseases that fish can be increased and the fertility for improving fish, extensive market, and phaffiafhodozyma is not
Only high-yield astaxanthin, can improve the vigor of laccase when co-culturing with the edible and medicinal fungi for producing laccase, and then raising utilizes cellulose
With the ability of the material such as lignin.Further, since different edible and medicinal fungis have different biological activitys, product of the present invention both may be used
Single application, the species, growth stage also dependent on animal carries out product composite usage.Product of the present invention prepares and should
Simple with method, with low cost, effect is significant, while comprehensively utilizing to tea grounds, reduces environmental pollution.
Specific embodiment
Tea grounds culture medium：The tea grounds 5g of drying, by every gram of tea grounds 30mg containing glucose, Magnesium sulfate heptahydrate 6mg, biphosphate
Potassium 9mg, vitamin B 1.5mg prepare nutritional solution.
Edible and medicinal fungi seed culture medium (gL-1)：Glucose 20, Semen Maydis powder 10, wheat bran 10, Magnesium sulfate heptahydrate 2, phosphoric acid
Potassium dihydrogen 3, initial pH natures.
Phaffiafhodozyma seed culture medium (gL-1)：Peptone 20, yeast extract 10, glucose 20, pH natures.
Edible and medicinal fungi seed culture：Take 0.5cm2The truffle of size, is inoculated in (250ml tri- in 80mL seed culture mediums
Angle bottle), 150rmin-1, 25 DEG C of cultivation temperature, culture 7d obtain seed liquor.
Phaffiafhodozyma seed culture：Drawn with inoculating loop and take appropriate phaffiafhodozyma, in being inoculated in 80mL fluid mediums,
200r·min-1, 30 DEG C of culture 48h.
Crude protein is determined：Kjeldahl's method.
Tea polyphenols are determined：GB/T 8313-2008.
Polyose extraction and measure：1g samples plus 10ml deionized waters are taken, 90 DEG C of extraction 1h, centrifugation takes and reset and add on 1m 4ml
95% industrial alcohol precipitate with ethanol, is overnight centrifuged afterwards, and precipitation is dissolved in water, and phend-sulphuric acid surveys polyoses content.
Astaxanthin extraction and measure：Take the wet samples of 100g after fermentation ends, after multigelation 7-8 time, plus the vibration of 150ml chloroforms
Extract 15 minutes, chloroformic solution is placed on decompression Rotary Evaporators after separation is concentrated into 10ml, be prepared into the chlorine of astaxanthin-containing
Imitative extracting solution.Using LC-MS detections content astaxanthin (see patent 201610507284.2), astaxanthin in dry weight is finally converted into
Content.
Embodiment 1：The different fermentations time is to tea polyphenols, polysaccharide, crude protein and content astaxanthin in glossy ganoderma fermentation tea grounds
Affect
By Ganoderma seed liquor inoculation 1.5ml (7.5%) in tea grounds culture medium, water content 70%, 25 DEG C of cultivation temperature, point
Not fermentation it is initial, tea polyphenols, polysaccharide, thick egg in tea grounds is measured by sampling within the 5th day, the 10th day, the 15th day, the 20th day, the 25th day
The content of white and astaxanthin.
Impact of the different fermentations time of table 1 to tea polyphenols, polysaccharide, crude protein and content astaxanthin in glossy ganoderma fermentation tea grounds.
Note：- expression is not detected by
Embodiment 2：The different fermentations time co-cultures tea polyphenols in fermentation tea grounds, polysaccharide, thick to Ganoderma and phaffiafhodozyma
The impact of albumen and content astaxanthin
Ganoderma and phaffiafhodozyma seed liquor are pressed into 1:After the mixing of 1.2 ratios, inoculation 1.5ml (7.5%) is in tea grounds culture
In base, water content 70%, 25 DEG C of cultivation temperature, respectively fermentation it is initial, be measured by sampling in tea grounds within the 5th day, the 10th day, the 15th day
The content of tea polyphenols, polysaccharide, crude protein and astaxanthin.
The different fermentations time of table 2 Ganoderma and phaffiafhodozyma are co-cultured tea polyphenols in fermentation tea grounds, polysaccharide, crude protein and
The impact of content astaxanthin.
Embodiment 3：Different Ganodermas and phaffiafhodozyma seed liquor mixed proportion co-culture tea polyphenols in fermentation tea grounds, many
The impact of sugar, crude protein and content astaxanthin
Ganoderma and phaffiafhodozyma seed liquor are pressed after different proportion mixing, 1.5ml is in tea grounds culture medium for inoculation, aqueous
Amount 70%, 30 DEG C of cultivation temperature, tea polyphenols, polysaccharide, crude protein and astaxanthin contains in fermentation is measured by sampling tea grounds on the 10th day
Amount.
The different Ganodermas of table 3 and phaffiafhodozyma seed liquor mixed proportion co-culture tea polyphenols, polysaccharide, thick egg in fermentation tea grounds
The impact of white and content astaxanthin.
Embodiment 4：Different vaccination amount co-cultures the shadow of tea polyphenols, polysaccharide, crude protein and content astaxanthin in fermentation tea grounds
Ring
Ganoderma and phaffiafhodozyma seed liquor are pressed into 1:1.2 ratios mixing after, be inoculated with different volumes seed mixture liquid in
In tea grounds culture medium, water content 70%, 30 DEG C of cultivation temperature, the tea polyphenols, polysaccharide, thick in fermentation is measured by sampling tea grounds on the 10th day
The content of albumen and astaxanthin.
The different vaccination amount of table 4 co-cultures the impact of tea polyphenols, polysaccharide, crude protein and content astaxanthin in fermentation tea grounds.
Embodiment 5：Different moisture content co-cultures the shadow of tea polyphenols, polysaccharide, crude protein and content astaxanthin in fermentation tea grounds
Ring
Ganoderma and phaffiafhodozyma seed liquor are pressed into 1:After 1.2 mixing, 1.5ml is in tea grounds culture medium for inoculation, water content
Respectively 50%, 60%, 70%, 80%, 30 DEG C of cultivation temperature, fermentation the 10th day be measured by sampling tea grounds in tea polyphenols, polysaccharide,
The content of crude protein and astaxanthin.
The different moisture content of table 5 co-cultures the impact of tea polyphenols, polysaccharide, crude protein and content astaxanthin in fermentation tea grounds.
Embodiment 6：Different edible and medicinal fungis co-culture tea polyphenols, polysaccharide, crude protein in fermentation tea grounds with phaffiafhodozyma
With the impact of content astaxanthin
Respectively Pnoliota adiposa(Fr.) Quel., Phellinus igniarius (L. ex Fr.) Quel., Ganoderma, Cordyceps and phaffiafhodozyma seed liquor are pressed into 1:After 1.2 mixing, inoculation
In tea grounds culture medium, water content is respectively 70% to 1.5ml, and cultivation temperature is 25 DEG C, tea grounds is measured by sampling within the 10th day in fermentation
The content of middle tea polyphenols, polysaccharide, crude protein and astaxanthin.
It is blue or green that the different edible and medicinal fungis of table 6 co-culture tea polyphenols, polysaccharide, crude protein and shrimp in fermentation tea grounds with phaffiafhodozyma
The impact of cellulose content.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclosing should be by being defined that claims are defined.
Claims (8)
1. a kind of method that fermentation tea grounds prepares feed additive, it is characterised in that be using edible and medicinal fungi and red husband's ferment
Female (Phaffia rhodozyma) is co-cultured and is carried out appropriate solid fermentation to tea grounds, obtains feed additive.
2. method according to claim 1, it is characterised in that the appropriate solid fermentation is to control fermentation time for 5-15
My god, 20-25 DEG C of fermentation temperature；The edible and medicinal fungi includes Pnoliota adiposa(Fr.) Quel. (Pholiota adiposa), Phellinus igniarius (L. ex Fr.) Quel. (Phellinus
Igniarius), one kind in Ganoderma (Ganoderma Lucidum), Cordyceps (Ophiocordyceps sinensis)
Or it is various.
3. method according to claim 1 and 2, it is characterised in that the tea grounds is green tea, black tea, Ramulus et Folium Mussaendae Pubescentis or oolong tea
Residue after effective component extracting.
4. method according to claim 3, it is characterised in that the tea grounds is green tea tea grounds.
5. according to the arbitrary described method of Claims 1 to 4, it is characterised in that comprise the steps：
1) process of tea grounds：It is standby after tea grounds drying, culture medium is directly used as, or it is used as culture medium after crushing；
2) preparation of nutritional solution：By every gram of tea grounds, addition glucose 20-40mg, Magnesium sulfate heptahydrate 3-9mg, biphosphate are weighed
Potassium 6-12mg, vitamin B 1-2mg, prepare the nutritional solution of water content 50-80%；
3) preparation of tea grounds culture medium：Will be standby after nutritional solution high temperature sterilize；
4) bacterium solution is prepared：By cultured edible and medicinal fungi seed liquor and phaffiafhodozyma seed liquor by volume 1:1-1.5 than
Example mixing；
5) it is inoculated with：By the 5-10% inoculation bacterium solutions of the weight in wet base quality of tea grounds culture medium；
6) ferment：At 20-25 DEG C, ferment 5-15 days；
7) mixture after fermentation ends is crushed after 70-80 DEG C of drying, you can use as feed additive.
6. the feed additive for being prepared according to the arbitrary described method of Claims 1 to 5.
7. the method for the feed additive described in a kind of application claim 6, it is characterised in that should as single fermentation product
With, or for the compounding of multiple product.
8. method according to claim 7, it is characterised in that addition of the feed additive in feedstuff is dry weight 0.5-
20%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201611214808.5A CN106666101A (en) | 2016-12-26 | 2016-12-26 | Feed additive prepared from fermented tea residues and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611214808.5A CN106666101A (en) | 2016-12-26 | 2016-12-26 | Feed additive prepared from fermented tea residues and application thereof |
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| CN106666101A true CN106666101A (en) | 2017-05-17 |
Family
ID=58870731
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| CN201611214808.5A Pending CN106666101A (en) | 2016-12-26 | 2016-12-26 | Feed additive prepared from fermented tea residues and application thereof |
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| CN107373038A (en) * | 2017-09-18 | 2017-11-24 | 永州市掌望生态农业有限公司 | A kind of fermented feed containing probiotics for improving egg quality and preparation method thereof |
| CN108077573A (en) * | 2017-12-11 | 2018-05-29 | 郑子聪 | One boar food and the health promoting liquid for pannage addition |
| CN110495520A (en) * | 2019-08-23 | 2019-11-26 | 福建师范大学 | Tea grounds fermentation liquid and its preparation method and application |
| CN112617016A (en) * | 2020-12-22 | 2021-04-09 | 宜兴市天石饲料有限公司 | Compound feed prepared from effective components in tea |
| CN114009625A (en) * | 2021-10-18 | 2022-02-08 | 南京师范大学 | Aquaculture feed and preparation method thereof |
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| CN112617016A (en) * | 2020-12-22 | 2021-04-09 | 宜兴市天石饲料有限公司 | Compound feed prepared from effective components in tea |
| CN114009625A (en) * | 2021-10-18 | 2022-02-08 | 南京师范大学 | Aquaculture feed and preparation method thereof |
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