CN102329833A - Method for producing sophorose ester through fermenting waste molasses and waste glycerin - Google Patents
Method for producing sophorose ester through fermenting waste molasses and waste glycerin Download PDFInfo
- Publication number
- CN102329833A CN102329833A CN201110170433A CN201110170433A CN102329833A CN 102329833 A CN102329833 A CN 102329833A CN 201110170433 A CN201110170433 A CN 201110170433A CN 201110170433 A CN201110170433 A CN 201110170433A CN 102329833 A CN102329833 A CN 102329833A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- liquid
- fermentation
- seed culture
- inoculated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for producing sophorose ester through fermenting waste molasses and waste glycerin, which comprises the following implementation steps that: (1) spore suspension preparation: torulopsis utilis TJZKBA 10326 is inoculated on the inclined surface of a culture medium and is cultured for 2 to 4 days at the constant temperature being 30 DEG C, and normal saline with the mass concentration being 0.75 percent is added for washing mycelia, and the bacterial suspension is obtained; (2) the bacterial suspension is used for being inoculated into the sterilized liquid seed culture medium, the spore suspension is inoculated into the cooled liquid seed culture medium according to the inoculation quantity with the volume percentage being 2 to 5, and liquid seeds are obtained; (3) the liquid seed culture medium is used for being inoculated into a sterilized 5L fermentation tank, then, the liquid culture medium is inoculated into the cooled fermentation tank culture medium according to the inoculation quantity with the volume percentage being 2 to 5, the fermentation temperature is 30 to 34 DEG C, the dissolved oxygen is controlled to be 30 to 40 percent, and the fermentation time is 48 to 72h; and (4) after the fermentation is completed, the mixed organic solvent of organic solvent ethyl acetate/isopropanol with the same volume as the fermentation liquid is used for extracting the sophorose ester. The method has the advantages that the process is simple, the cost is low, and the effect is obvious.
Description
Technical field
The present invention relates to a kind of method of fermentative prodn sophorolipid, particularly a kind of method
of producing the sophorose ester with waste molasses and useless glycerol fermentation.
Background technology
Soy molasses is to produce the sub product that obtains in the soybean protein concentrate process, contains abundant soybean oligosaccharide in the soy molasses, can be used as the raw materials for production of functional soy oligose.Wherein staple content is: total reducing sugar 54.64% (wherein sucrose 32.17 %, cottonseed sugar 4.27%, stachyose 17.54%), total lipoid 18.00%, total steroid glucoside 3.06%, total phospholipids 8.79%, crude protein 8.71%, ash content 8.69%.
Glycerine has another name called USP Kosher, is a kind of colourless, odorless, the sweet thick liquid of flavor.The chemical structure of glycerine is different fully with glucide, thereby does not belong to same type of material.Useless glycerine source is wide, and price is lower.Be mainly derived from Soap Factory and biofuel factory, directly discharging pollutes the environment, if be used, with making waste product convert the higher glycerol product of economic worth to, it is egoistic to benefit the nation.
Sophorolipid (
Sophorolipid) be a kind of degradable biological tensio-active agent.Bio-surfactant has higher selectivity, and its surfactivity receives condition influence such as extreme temperature, pH value, salinity little.Compare with other tensio-active agent of producing through chemosynthesis or refining of petroleum method, bio-surfactant has broad application prospects in fields such as food, makeup, medicine and environment protection.Sophorolipid is as wherein important a member; Toxicity is low; Good with human body and Environmental compatibility; Have characteristics such as good emulsifying, dispersion, solubilising, have research and development in fields such as food, medicine, makeup, metallurgy, environment protection, waste oil recovery and be worth, be applied to daily use chemicals, foodstuffs industry, petroleum industry etc. at present.In the world sophorolipid theoretical investigation and application and development have been carried out for many years; America and Europe and some scholars of Korea S find after deliberation recently; Sophorolipid has certain bacteriostatic action; And can the Imidazolidinyl Urea epidermic cell and the growth of dermal fibroblast, these researchs have very important significance to effective application of sophorolipid.China also is in the starting stage in this field at present.
Usually adopt the method for vegetables oil fermentative prodn sophorolipid at present, because the vegetables oil price is higher, fermentation period is longer; Production technique is complicated; It is bigger to add back extraction process rate of loss, so the yield of sophorolipid is also lower, causes the cost of sophorolipid higher; Market value is also higher, is unfavorable for applying very much of sophorolipid.
Therefore, a kind of efficient feasible method with waste molasses and useless glycerol fermentation production sophorolipid being provided, is one of this technical field scientific research personnel new problem of being badly in need of developing.
Summary of the invention
The objective of the invention is to overcome the weak point of above-mentioned prior art, provide a kind of efficient feasible and can reduce the sophorolipid production cost, reduce investment, benefit the method with waste molasses and useless glycerine production sophorolipid of production application.
Following for realizing the embodiment that above-mentioned purpose the present invention adopted: a kind of method with waste molasses and useless glycerol fermentation production sophorose ester is characterized in that implementation step is following:
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the medium slant with the torulopsis bacterium, and in 30 ℃ of constant temperature culture 2-4 days, the adding mass concentration was 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 10
5-10
6Individual, for use; Slant medium is formed and is comprised the 1L of unit: glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5; Wherein used bacterial classification: torulopsis bacterium (Torulopsis bombicola) TJZKBA10326;
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seed culture medium is formed and comprised the 1L of unit: glucose content 20-60g; Semen Maydis oil content 20-60g, yeast powder 4-8g, urea 0.5-1g; Be settled to 1L with zero(ppm) water, pH value 3.5; With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume percent 2-5% inserts spore suspension in the above-mentioned refrigerative liquid seed culture medium then; The bottled liquid measure of shaking of 500ml specification is 100ml; Shake on the shaking table that bottle is 100-150rpm in 30-34 ℃, rotating speed and cultivate 24h, obtain liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 240-360g, and waste molasses dilution 1-5 doubly is settled to 3L with zero(ppm) water; PH value 4.0-4.5, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 2-5% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, and leavening temperature 30-34 ℃, dissolved oxygen is controlled at 30-40%, fermentation time 48-72h;
(4) after the fermentation ends, use and the isopyknic organic solvent ethyl acetate/Virahol of fermented liquid both ratio 4:1 of said ethyl acetate/Virahol; The v/v of unit; Mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, and obtaining sophorolipid output is 40-80g/L.
The invention has the beneficial effects as follows: the present invention adopts waste molasses and useless glycerol fermentation to produce sophorolipid technology and can reduce production costs greatly; Both solved the big problem of waste molasses and useless glycerine exhaust emission not easy to store; Having produced sophorolipid with lower cost again, is to kill two birds with one stone, benefit the nation and the people.And do not need complex apparatus, treatment process is very simple, and fermenting process is simple, is beneficial to the popularization of the Application and Development of sophorolipid.But this method producing sophorolipid by continuous feeding and fermentation is of very high actual application value, and the market advantage is very obvious.
Embodiment
Below in conjunction with embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the medium slant with the torulopsis bacterium, in 30 ℃ of constant temperature culture 2 days, adds mass concentration and be 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 3.6 * 10
5Individual, for use; Slant medium is formed and is comprised (1L): glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5.Wherein used bacterial classification is: torulopsis bacterium (Torulopsis bombicola) TJZKBA10326.
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seed culture medium is formed and comprised (1L): glucose 30g, and Semen Maydis oil 30g, yeast powder 5g, urea 0.5g is settled to 1L with zero(ppm) water, pH value 3.5.With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume percent 2% inserts (the bottled liquid measure of shaking of 500ml specification is 100ml) in the above-mentioned refrigerative liquid seed culture medium with spore suspension then, shakes on the shaking table that bottle is 100rpm in 30 ℃, rotating speed and cultivates 24h, obtains liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 240g, and 5 times of waste molasses dilutions are settled to 3L with zero(ppm) water; PH value 4.3, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 3% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, 34 ℃ of leavening temperatures, and dissolved oxygen is controlled at 40%, fermentation time 48h;
(4) after the fermentation ends, use that (ethyl acetate/Virahol ratio is 4:1, and v/v), its mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, and obtaining sophorolipid output is 41g/L with the isopyknic ethyl acetate/Virahol of fermented liquid.
Embodiment 2
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the inclined-plane with the torulopsis bacterium, in 30 ℃ of constant temperature culture 2 days, adds mass concentration and be 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 4.5 * 10
5Individual, for use; Slant medium is formed and is comprised (1L): glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5.
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seed culture medium is formed and comprised (1L): glucose 50g, and Semen Maydis oil 50g, yeast powder 7g, urea 0.8g is settled to 1L with zero(ppm) water, pH value 3.5.With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 4% inserts (the bottled liquid measure of shaking of 500ml specification is 100ml) in the above-mentioned refrigerative liquid seed culture medium with spore suspension then, shakes on the shaking table that bottle is 120rpm in 32 ℃, rotating speed and cultivates 24h, obtains liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 300g, and 4 times of waste molasses dilutions are settled to 3L with zero(ppm) water; PH value 4.5, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 2% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, 34 ℃ of leavening temperatures, and dissolved oxygen is controlled at 35%, fermentation time 60h;
(4) after the fermentation ends, use with the isopyknic ethyl acetate/Virahol of fermented liquid (4:1, v/v) mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, obtaining sophorolipid output is 61g/L.
Other is with embodiment 1.
Embodiment 3
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the inclined-plane with the torulopsis bacterium, in 30 ℃ of constant temperature culture 3 days, adds mass concentration and be 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 5.8 * 10
5Individual, for use; Slant medium is formed and is comprised (1L): glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5.
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seed culture medium is formed and comprised (1L): glucose 60g, and Semen Maydis oil 60g, yeast powder 8g, urea 1g is settled to 1L with zero(ppm) water, pH value 3.5.With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 3% inserts (the bottled liquid measure of shaking of 500ml specification is 100ml) in the above-mentioned refrigerative liquid seed culture medium with spore suspension then, shakes on the shaking table that bottle is 150rpm in 34 ℃, rotating speed and cultivates 24h, obtains liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 330g, and 3 times of waste molasses dilutions are settled to 3L with zero(ppm) water; PH value 4.0, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 4% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, 30 ℃ of leavening temperatures, and dissolved oxygen is controlled at 30%, fermentation time 60h;
(4) after the fermentation ends, use with the isopyknic ethyl acetate/Virahol of fermented liquid (4:1, v/v) mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, obtaining sophorolipid output is 72g/L.
Other is with embodiment 1.
Embodiment 4
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the inclined-plane with the torulopsis bacterium, in 30 ℃ of constant temperature culture 4 days, adds mass concentration and be 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 7.6 * 10
5Individual, for use; Slant medium is formed and is comprised (1L): glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5.
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seeds is cultivated
Base is formed and is comprised (1L): glucose 20g, and Semen Maydis oil 20g, yeast powder 4g, urea 0.6g is settled to 1L with zero(ppm) water, pH value 3.5.With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 5% inserts (the bottled liquid measure of shaking of 500ml specification is 100ml) in the above-mentioned refrigerative liquid seed culture medium with spore suspension then, shakes on the shaking table that bottle is 110rpm in 30 ℃, rotating speed and cultivates 24h, obtains liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 360g, and 1 times of waste molasses dilution is settled to 3L with zero(ppm) water; PH value 4.0, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 4% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, 34 ℃ of leavening temperatures, and dissolved oxygen is controlled at 30%, fermentation time 72h;
(4) after fermentation 72h finishes, use with the isopyknic ethyl acetate/Virahol of fermented liquid (4:1, v/v) mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, obtaining sophorolipid output is 79.6g/L.
Above-mentioned detailed description of the method for producing the sophorose ester with waste molasses and useless glycerol fermentation being carried out with reference to embodiment; Be illustrative rather than determinate; Can be according to institute's limited range several embodiment that give an example out; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (1)
1. method of producing the sophorose ester with waste molasses and useless glycerol fermentation is characterized in that implementation step is following:
(1) preparation of spore suspension: TJZKBA10326 is inoculated on the medium slant with the torulopsis bacterium, and in 30 ℃ of constant temperature culture 2-4 days, the adding mass concentration was 0.75% saline water washing mycelium, obtains bacteria suspension, and suspension miospore content is about 10
5-10
6Individual, for use; Slant medium is formed and is comprised the 1L of unit: glucose 8g, and malt meal 3g, peptone 4g, yeast powder 4g, agar 15g is settled to 1L with zero(ppm) water, pH value 3.5; Wherein used bacterial classification: torulopsis bacterium TJZKBA10326;
(2) above-mentioned bacteria suspension is used for being seeded to the liquid seed culture medium after the sterilization, and liquid seed culture medium is formed and comprised the 1L of unit: glucose content 20-60g; Semen Maydis oil content 20-60g, yeast powder 4-8g, urea 0.5-1g; Be settled to 1L with zero(ppm) water, pH value 3.5; With liquid seed culture medium in 121 ℃, 15min sterilization cooling again; Inoculum size according to volume percent 2-5% inserts spore suspension in the above-mentioned refrigerative liquid seed culture medium then; The bottled liquid measure of shaking of 500ml specification is 100ml; Shake on the shaking table that bottle is 100-150rpm in 30-34 ℃, rotating speed and cultivate 24h, obtain liquid seeds;
(3) the aforesaid liquid seed culture medium is used for being seeded to the 5L fermentor tank after the sterilization; Fermentation tank culture medium is formed and is comprised: useless glycerine 240-360g, and waste molasses dilution 1-5 doubly is settled to 3L with zero(ppm) water; PH value 4.0-4.5, with fermentor cultivation based on 121 ℃, 15min sterilization cooling again; Inoculum size according to volume ratio 2-5% inserts liquid nutrient medium in the above-mentioned refrigerative fermentation tank culture medium then, and leavening temperature 30-34 ℃, dissolved oxygen is controlled at 30-40%, fermentation time 48-72h;
(4) after the fermentation ends, use and the isopyknic organic solvent ethyl acetate/Virahol of fermented liquid both ratio 4:1 of said ethyl acetate/Virahol; The v/v of unit; Mixed organic solvents extracts sophorolipid, and vacuumizes at 70 ℃ and to remove organic solvent, and obtaining sophorolipid output is 40-80g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110170433A CN102329833A (en) | 2011-06-23 | 2011-06-23 | Method for producing sophorose ester through fermenting waste molasses and waste glycerin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110170433A CN102329833A (en) | 2011-06-23 | 2011-06-23 | Method for producing sophorose ester through fermenting waste molasses and waste glycerin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102329833A true CN102329833A (en) | 2012-01-25 |
Family
ID=45481795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110170433A Pending CN102329833A (en) | 2011-06-23 | 2011-06-23 | Method for producing sophorose ester through fermenting waste molasses and waste glycerin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102329833A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103540631A (en) * | 2013-10-22 | 2014-01-29 | 山东大学 | Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates |
| CN103589759A (en) * | 2013-11-22 | 2014-02-19 | 青岛科技大学 | Method for producing sophorolipid by double carbon source fermentation of waste molasses and illegal cooking oil |
| CN103805525A (en) * | 2012-11-09 | 2014-05-21 | 丰益(上海)生物技术研发中心有限公司 | Saccharomyces cerevisiae strain and application |
| CN107177643A (en) * | 2017-06-30 | 2017-09-19 | 广州美中生物科技有限公司 | White fungus slag charge tunning, skin care articles for washing containing the tunning and preparation method thereof |
-
2011
- 2011-06-23 CN CN201110170433A patent/CN102329833A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103805525A (en) * | 2012-11-09 | 2014-05-21 | 丰益(上海)生物技术研发中心有限公司 | Saccharomyces cerevisiae strain and application |
| CN103805525B (en) * | 2012-11-09 | 2018-02-16 | 丰益(上海)生物技术研发中心有限公司 | A kind of saccharomyces cerevisiae bacteria strain and application |
| CN103540631A (en) * | 2013-10-22 | 2014-01-29 | 山东大学 | Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates |
| CN103540631B (en) * | 2013-10-22 | 2015-04-22 | 山东大学 | Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates |
| CN103589759A (en) * | 2013-11-22 | 2014-02-19 | 青岛科技大学 | Method for producing sophorolipid by double carbon source fermentation of waste molasses and illegal cooking oil |
| CN103589759B (en) * | 2013-11-22 | 2016-01-13 | 青岛科技大学 | A kind of waste molasses and the two carbon source through fermentation of sewer oil produce the method for sophorolipid |
| CN107177643A (en) * | 2017-06-30 | 2017-09-19 | 广州美中生物科技有限公司 | White fungus slag charge tunning, skin care articles for washing containing the tunning and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108823261A (en) | A kind of Ultra-low molecular weight Dendrobium officinale polysaccharide and its preparation and application | |
| CN101988036B (en) | Aureobasidium pullulans strain as well as preparation method and application thereof in producing pigment-free pullulan | |
| CN101589151A (en) | Method for production of ethanol or lactic acid | |
| CN102599004A (en) | Method for culturing Cordyceps Sinensis mycelia with hemp seed-containing culture medium | |
| CN105969673A (en) | Fermentation method for increasing yield of haematochrome and uranidin in monascus purpureus | |
| CN101831471A (en) | Method for producing polysaccharides by fermenting yellow serofluid with edible and medicinal fungi | |
| CN103571756A (en) | Test tube screening method of isaria cicadae strain and culture medium | |
| CN101358173B (en) | Aspergillus niger ZJUT712 and application thereof in arctium fruit preparation by solid-state fermentation | |
| CN102329833A (en) | Method for producing sophorose ester through fermenting waste molasses and waste glycerin | |
| CN101845469A (en) | Method for producing sophorolipid by continuous feeding and fermentation | |
| CN102634460A (en) | Rhizopus oryzae RH1-5 and separating and culturing method thereof | |
| CN102433288B (en) | Strain for producing ornithine and method for biologically synthesizing ornithine with same | |
| CN103589759A (en) | Method for producing sophorolipid by double carbon source fermentation of waste molasses and illegal cooking oil | |
| CN103627513A (en) | Fed-batch pretreatment solid-state fermentation auxiliary cold-pressing oil extraction process for oil crops | |
| CN103614429B (en) | Method for transforming gingko pollen flavonoid glycoside employing microorganisms | |
| CN103725738B (en) | The method preparing collagen polypeptide with Carnis Pseudosciaenae leftover bits and pieces | |
| CN110551636B (en) | Monascus purpureus MY-21 strain and application thereof | |
| CN101805760A (en) | Method for producing microbial oil comprising arachidonic acid | |
| KR20100135455A (en) | Method for mass production of microbial cellulose and method for mask pack using there of | |
| CN106636252A (en) | Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide | |
| CN115039636B (en) | Culture medium for cultivating tremella and preparation method thereof | |
| CN103333872B (en) | Method for preparing Beta-glucuronidase crude enzyme preparation | |
| CN108796027A (en) | A method of producing carotenoid | |
| CN112143770B (en) | Marine rhodotorula and application thereof in producing beta-carotene by taking straw as raw material | |
| CN101497902A (en) | Process for preparing microbe oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120125 |