CN107177643A - White fungus slag charge tunning, skin care articles for washing containing the tunning and preparation method thereof - Google Patents
White fungus slag charge tunning, skin care articles for washing containing the tunning and preparation method thereof Download PDFInfo
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- CN107177643A CN107177643A CN201710527932.5A CN201710527932A CN107177643A CN 107177643 A CN107177643 A CN 107177643A CN 201710527932 A CN201710527932 A CN 201710527932A CN 107177643 A CN107177643 A CN 107177643A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
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- A—HUMAN NECESSITIES
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a kind of white fungus slag charge tunning, the washing and skin care articles for use containing the tunning and preparation method thereof, the white fungus slag charge tunning enters yeast starter liquid pump in fermentation medium, 25~32 DEG C ferment 5~8 days, terminate fermentation when glucose pol is less than 0.5g/L, collect tunning and obtain, the skin care articles for washing includes white fungus slag charge tunning and white fungus slag charge.The present invention is used as one of composition of washing and skin care articles for use using the biosurfactant sophorolipid in white fungus slag charge tunning, compared with adding the skincare product of surfactant of chemical synthesis, with lower toxicity, higher biological degradability, more preferable Environmental compatibility, higher foaming characteristic, there are more preferable selectivity and selectivity under extreme temperature, pH, salinity, it is higher to skin compatibility, strong impulse will not be produced to skin, product is safer during use.
Description
Technical field
The invention belongs to Applied Biotechnology field, more particularly it relates to a kind of white fungus slag charge tunning and
Its preparation method and washing and skin care articles for use containing the white fungus slag charge tunning and preparation method thereof.
Background technology
Surfactant is essential a kind of component in washing and skin care product raw material, plays wetting, emulsification, foaming, increases
Thick, solubilized, decontamination etc. are acted on, using extremely extensive in actual production.
The surfactant that washing and skin care product in the market are added is generally artificial chemistry synthesis.Such chemical surface
Activating agent often causes different degrees of pollution in actual production to environment, such as changes the physicochemical property of soil, contaminant water
Body etc., and be difficult to be degraded.Upper using, some chemical surfactants can carry out stimulation to user's skin tape, draw
Play the symptoms such as red swelling of the skin, inflammation, allergy.
Biosurfactant is the metabolite with surface-active that microorganism is metabolized generation under certain condition, bag
Include glycolipid, saccharose lipid, lipopeptid or neutral lipid derivative etc..Biosurfactant not only has as chemical surfactant
There are various surface propertys, but also the good characteristic that can not be matched in excellence or beauty with the latter：Applicability is wide；Bio-toxicity is extremely low, to environment
It is friendly；Suitable for extreme temperature, pH and salinity；Molecular structure type is various, and some types have many specific functional groups, surface
Function admirable.
Sophorolipid is the most biosurfactant of exploitation prospect at this stage, one is because its yield and
Conversion ratio is higher than other biosurfactants；And another it is important the reason for be then, its main competitor --- sandlwood
The producing strains of glycolipid, be with pathogenic Pseudomonas aeruginosa, and sophorolipid production bacterium be non-pathogenic saccharomycete.Chinese scholartree
Glycolipid belongs to the secondary metabolite of microorganism, not only with the common of surfactant as a kind of biosurfactant
Feature (such as wetting action, osmosis, barbotage, solubilization), also has unique physicochemical property, mainly including following
Several aspects：
First, sophorolipid has excellent surface-active.Sophorolipid belongs to nonionic surface active agent, molecular structure bag
Containing two groups：The most long-chain saturations by 16~18 carbon of one hydrophilic radical and a hydrophobic grouping, wherein hydrophobic grouping
Or unsaturated hydroxyl fatty acid chain composition, it can be uniformly distributed in aqueous and arrange stratification, substantially reduce surface tension.
There is pertinent literature report when sophorose lipid concentration is 40~100mg/L in the aqueous solution, surface tension can be reduced to 30~40mN/m.
In addition, the critical micelle concentration and Interfacial Tension Behavior of sophorolipid are relatively low, it can relatively easily change system after being added in solution
Surface nature.
Second, sophorolipid has good chemical stability and heat endurance.Studies have reported that sophorolipid is in 90 DEG C of constant temperature
After water-bath 1h, the characteristic of emulsifying capacity and reduction surface tension is determined, finds to have almost no change.In addition, even in high soda acid
And under conditions of high salt, sophorolipid remains to keep superperformance.
3rd, sophorolipid is to the nontoxic close friend of ecological environment.Sophorolipid belongs to the natural products of microbial fermentation, and it was synthesized
Journey is the process of microbial metabolism and enzymatic, and reaction condition is gentle, and waste residue generation is not had, hardly shadow is produced to environment
Ring.Sophorolipid has nontoxic, tasteless and nonirritant characteristic, and is finally recycled to be biodegradable in natural environment and disappears
Consume, do not have pollution products.
4th, sophorolipid molecular structure complexity is abundant, to causing rotten bacterium to have certain inhibitory action.Sophorolipid has more than 20
The analogue planted, and also have a large amount of new sophorolipids to be constantly found.There are some researches show the sophorose of 0.5g/L concentration
Fat can almost completely inhibit the growth of saccharomycete, the inhibiting rate for also having 60% to mould.The sophorolipid of 2.0g/L concentration is to mould
Inhibiting rate more than 80%.Sophorolipid is respectively provided with good inhibitory action to the microorganism in fungal hyphae and air simultaneously.
3.0g/L sophorolipid can suppress in air more than 95% microorganism, and inhibiting rate is spread to mycelia also above 70%；In addition,
Sophorolipid can also suppress and postpone mycelia and produce spore.
White fungus is also known as tremella, tremella, white fungus etc., is a kind of high, the extremely precious edible mushroom of economic value and medicine
With bacterium, belong to traditional product for food and medicine purposes.White fungus producing region is extremely wide, be mainly distributed on benign climate growth Sichuan, Zhejiang,
It is Fujian, Jiangsu, Jiangxi, Anhui, Taiwan, Hubei, Hainan, Hunan, Guangdong, Hong Kong, Guangxi, Guizhou, Yunnan, Shaanxi, Gansu, interior
The areas such as Mongolia, Tibet.White fungus extracts acquisition tremella polysaccharides mainly as raw material in commercial Application, the master extracted at this stage
Method is wanted to have Hot water extraction；Alkali extraction method；Acid extraction method；Enzyme extraction method etc., finds with hot water extraction's fado after someone's research
Sugared yield highest；And Hot water extraction passes through multiple condition optimizing, polysaccharide yield also only has 16.81%.Therefore by having extracted
Still containing substantial amounts of using material in white fungus slag charge afterwards, with higher value.But most enterprises typically will not
White fungus slag charge is developed again, but selects directly to discharge discarded, this is to belong to waste of resource to a certain extent
Behavior.
In summary, biosurfactant product is although functional, safety and environmental protection, application direction have it is wide before
Scape, but its industrialization is still not yet ripe, and production integrated cost is higher.Therefore still make in most of articles for washing on the market
With the surfactant of chemical synthesis, and the articles for washing species that with the addition of biosurfactant is rare, and expensive,
Population is difficult to receive.If can be obtained using cheap, wide material sources raw materials to produce with a variety of good characteristics
Biosurfactant, and it is processed into a zinc cation articles for use, then it can greatly reduce production cost, improve enterprise
Benefit, while also can industrial by-products be carried out with secondary utilization, substantially increases resource utilization, is truly meeting
The strategy of sustainable development theory of " energy-saving and emission-reduction ".
Chinese patent《A kind of preparation methods of microbial detergent of CN201310077379.1》Disclose a kind of containing biology
The preparation method of the detergent of surfactant：Using pseudomonas aeruginosa fermenting and producing rhamnolipid, bacillus subtilis is utilized
Bacterium fermenting and producing lipopeptid, by 5~15 parts of rhamnolipid extract, lipopeptid extract, 0.01~0.05 part of 10000u/g lipase,
1~10 part of sucrose fatty ester, EDTA0.5~1.5 part mixing, produce microbial detergent finished product.Wherein, rhamnolipid and fat
Peptide is biosurfactant, and sucrose fatty ester is the surfactant of chemical synthesis, and the lipase enhancing that reduces fat is washed
Effect is washed, EDTA is chelating agent.The method replaces the surfactant of traditional chemical industry synthesis using biosurfactant, very well
Eliminate the harmful effect that traditional table work is brought to environment, and biological table is lived safety, gentle, micro- to skin irritation
It is weak.The method that production process uses biofermentation, has fewer environmental impacts, and detergent is easily degraded, and does not result in secondary
Pollution, adapts to society to the skin compatibility of detergent, the requirement of environmentally friendly low-carbon.But in the microbial detergent preparation method
The fermentation medium composition of rhamnolipid is soybean oil, sunflower oil, yeast extract and salt, is still deposited in making full use of for resource
In certain defect.In addition, the producing strains of rhamnolipid are with highly pathogenic Pseudomonas aeruginosa, if later purification work
Skill is gone wrong, and tunning can be made to carry pathogenic bacteria, therefore there is the risk of pollution detergent, is brought seriously to user
Harm.
The content of the invention
Based on this, in order to overcome the defect of above-mentioned prior art, the invention provides a kind of white fungus slag charge tunning and
Its preparation method and its application in terms of skin care articles for washing.
In order to realize foregoing invention purpose, this invention takes following technical scheme：
A kind of preparation method of white fungus slag charge tunning, comprises the following steps：By 1~5% inoculum concentration, by yeast kind
Sub- liquid pump enters in the fermentation medium of pH5.5~6.5 of sterilizing, and 25~32 DEG C ferment 5~8 days, when pol is less than 0.5g/L
Terminate fermentation, collect tunning, produce, the fermentation medium contains 40~100g/L glucose, 30~80g/L jade
The white fungus slag charge of rice bran oil and 40~75% (v/v)；The temperature of the fermentation medium is 25~32 DEG C；The pol is with Portugal
Grape sugar meter.
In wherein some embodiments, the preparation method of the yeast starter liquid is：By the yeast inclined-plane seed activated
In the seed culture medium for being seeded to pH5.5~6.5 of sterilizing, 120~200rpm, 25~32 DEG C of 24~40h of culture；The seed
Culture medium includes 20~50g/L glucose and 15~40g/L corn oil.
In wherein some embodiments, the sterilizing is 115~121 DEG C, 15~30min of high-temperature sterilization.
Present invention also offers the white fungus slag charge tunning that above-mentioned preparation method is prepared.
Present invention also offers application of the above-mentioned white fungus slag charge tunning in terms of skin care articles for washing.
A kind of skin care articles for washing, the skin care articles for washing includes the raw material of following parts by weight：Above-mentioned white fungus slag charge hair
45~67.5 parts of ferment product, 22.5~45 parts of white fungus slag charge, 1~10 part of glycerine, 0~6.5 part of titanium dioxide.
In wherein some embodiments, the skin care articles for washing includes the raw material of following parts by weight：Above-mentioned white fungus slag charge
50~60 parts of tunning, 25~35 parts of white fungus slag charge, 4~6 parts of glycerine, 2~4 parts of titanium dioxide.
In wherein some embodiments, the skin care articles for washing also include methylchloroisothiazandnone 0.00027~
0.0012 part, 0.00009~0.0004 part of methylisothiazolinone, methylchloroisothiazandnone and methylisothiazolinone conduct
The preservative of product.
In wherein some embodiments, the weight ratio of the methylchloroisothiazandnone and methylisothiazolinone is 3：1.
In wherein some embodiments, the glycerine is that food grade glycerin, pharmaceutical grade glycerine or 99.5% regular grade are sweet
Oil, preferably food grade glycerin, glycerine play moist moisturizing.
In wherein some embodiments, the titanium dioxide is rutile titania, rutile or food-grade titanium dioxide, and titanium dioxide has higher
Tinting strength, tinting power and covering power, as Chinese white, rise whitening function.
In wherein some embodiments, the skin care articles for washing is shampoo, hand cleanser, shower cream or mildy wash.
Present invention also offers the preparation method of above-mentioned skin care articles for washing, comprise the following steps：
(1), by white fungus slag charge tunning, white fungus slag charge, glycerine and titanium dioxide 115~121 DEG C in autoclave, 0.07
15~45min of~0.11Mpa sterilizings；
(2), under aseptic condition, add through 0.22~0.45 μm of methylchloroisothiazandnone filtered and methylisothiozoline
Ketone, homogeneous is mixed, and is produced.
In wherein some embodiments, sterilization time described in step (1) is 20~35min.
In wherein some embodiments, homogeneous described in step (2) is mixed and aseptically carried out.
The present invention utilizes the accessory substance after the extracted polysaccharide of white fungus --- and white fungus slag charge carries out biofermentation, obtains containing life
The tunning of thing surfactant sophorolipid, and then processing prepares a efficiency natural, the biofermentation skin care of action temperature sum
Articles for washing, compared with prior art, the invention has the advantages that：
1st, the raw material of tunning of the invention comes from industrial waste white fungus material residue, and white fungus still has big after polysaccharide is extracted
Available material is measured, industrial waste is reclaimed and is developed, both saved production cost, be fully opening to resource again
Hair and utilization, reduce the discharge capacity of trade waste, improve the utilization rate of raw material, meet the sustainable development of " energy-saving and emission-reduction "
Open up Strategic Concept；
2nd, the present invention can also be produced in fermentation process, in microbial metabolism various enzymes, amino acid, vitamin,
Organic acid and peptide material, enzyme material have good degreasing, exfoliating ability, can be acted synergistically together with sophorolipid；
And the material such as amino acid, vitamin and polypeptide wherein contained can have nursing and the work(nourished concurrently while cleaning skin
Effect；
3rd, the present invention is used as the articless for washing such as shower cream, face cleaning dew using the gentle biosurfactant sophorolipid of safety
One of composition, sophorolipid is, using pure natural, edible white fungus slag charge as primary raw material, to be produced by microbial fermentation,
Sophorolipid have lower toxicity, higher biological degradability, more preferable Environmental compatibility, higher foaming characteristic, in extreme temperature
There are more preferable selectivity and selectivity under degree, pH, salinity, its performance is better than the surfactant of chemical industry synthesis；Moreover, sophorose
The skin compatibility of fat is higher, will not produce strong impulse to skin during use, advantage is had more in product promotion；It is raw
The strain for producing sophorolipid is the saccharomycete of no pathogenicity, has high cause a disease unlike producing the Pseudomonas aeruginosa of rhamnolipid
Property, in addition, the washing and skin care articles for use of the present invention are free of bleaching agent, fluorescent whitening agent, artificial essence, pigment etc. to the poisonous pair of human body
The composition of effect, these characteristics cause product safer.
Embodiment
The present invention is further discussed below with reference to specific embodiment, the present invention does not address part and is applied to prior art.Under
Face provides the specific embodiment of the present invention, but embodiment is merely to be described in further detail this explanation, is not intended to limit the present invention
Claim.Reagent or raw material used in following examples, unless otherwise specified, are derived from commercially available.
The term being related in the present invention is explained as follows：
Fermentation：Refer to by vital movement of the microorganism under aerobic or oxygen free condition prepare microbial cells in itself or
The process of microbial metabolic products.
Biosurfactant：Refer to the class material with surface-active produced by microbial fermentation, make an addition to
The effect such as thickening, solubilising, emulsification, foaming can be played in cosmetics.
Strain domestication：Refer to makes microorganism progressively adapt to a certain condition by artificial measures, and the side of directive breeding microorganism
Method.The bacterial strain with higher tolerance and mobility can be obtained by domestication.
Culture medium：The nutriment of artificial preparation used is grown and maintained for microorganism, plant tissue and animal tissue, typically
Contain carbohydrate, nitrogen substance, inorganic salts (including trace element) and vitamin and water etc..
White fungus slag charge：Slag charge of the white fungus after extracting polysaccharide.
The torulopsis inclined-plane seed strain source used in following examples is used as base in U.S. ATCC using white fungus slag charge
Basal culture medium, what optimization routine was screened and obtained.
A kind of skin care articles for washing of embodiment 1 and preparation method thereof
The skin care articles for washing of the present embodiment is shampoo, includes the raw material of following parts by weight：White fungus slag charge tunning
45 parts, 45 parts of white fungus slag charge, 5 parts of glycerine, 0.00027 part of methylchloroisothiazandnone, 0.00009 part of methylisothiazolinone.
The preparation method of the skin care articles for washing of the present embodiment, comprises the following steps：
1st, white fungus slag charge tunning is prepared
A, liquid seeds culture, domestication and expansion culture
Torulopsis inclined-plane seed that a ring activated is inoculated with super-clean bench in seed culture medium, 180rpm, 30 DEG C
24h is cultivated, seed culture medium is consisted of the following composition：Glucose 30g/L, corn oil 25g/L, with 0.10% (w/v) KH2PO4
And Na2HPO4Adjust pH to 6.0,115 DEG C of sterilizing 20min.
B, fermentation
Fermentation medium is prepared, fermentation medium is consisted of the following composition：Glucose 60g/L, corn oil 50g/L, finally
With white fungus slag charge and water constant volume, the wherein volume fraction of white fungus slag charge is 75%, with 0.10% (w/v) KH2PO4And Na2HPO4
PH to 6.0 is adjusted, is mixed using homogenizer, homogeneous.
It is added to after preparing in fermentation tank, 115 DEG C of sterilizing 30min are cooled to 30 DEG C.
Step a liquid seeds liquid is pumped into by 2% inoculum concentration, 30 DEG C ferment 6 days, are less than in pol (with glucose meter)
Terminate fermentation during 0.5g/L, collect tunning.
The sophorolipid in tunning is determined, using anthrone method, its principle for determining total reducing sugar is：Anthrone reaction method is a kind of
Fixed sugar method, free form or can whether be present in polysaccharide, various sugar for determining hexose, aldopentose, hexuronic acid
Blue-green is all presented.Under 620nm, maximum light absorption is presented.This method is rapidly, conveniently.Comprise the following steps that：
It is accurate to weigh anthrone reagent 0.1g, the 100ml concentrated sulfuric acids are added, are anthrone reagent after mixing.
Prepare 10mg/ml anhydrous grape sugar juice：Accurately the analytically pure DEXTROSE ANHYDROUSs of 0.1g are weighed (in advance 105
DEG C dry to constant weight), moved to after being dissolved with distilled water in 100ml volumetric flasks, and, so that whole. with distillation washing measuring cup for several times
Glucose is moved into volumetric flask, and last constant volume shakes up to scale, and its concentration is 1000mg/L.Then take 0.5 respectively, 1,2,3,
4ml is settled to 100ml, concentration is respectively 5,10,20,30,40mg/L.
Anthrone method draws glucose standard curve：Anthrone reagent 4ml is measured respectively and Standard glucose solution 1ml is placed in tool
Fill in test tube, cooled down in rapid immersion ice bath, then heated on boiling water bath under 10min, ice bath cooling, 620nm, use light splitting light
Degree measurement OD values (1ml distilled water is blank control).
Sophorolipid measuring principle：Sophorolipid is made up of sophorose and fat two parts, and determining sophorolipid sample with anthrone method exists
OD values at 620nm, the content of glucose is obtained according to OD values from the glucose standard curve that anthrone method is determined；Utilize sophorose
Ratio between fat and glucose molecule amount, i.e. 1g glucose are equivalent to 1.91g sophorolipids, so as to obtain sophorolipid in sample
Content.
Sophorolipid determination step：Tunning 1ml is taken, isometric 1ml ethyl acetate is added, 5min is stood after mixing,
5000rpm centrifuges 5min；10ul is taken to add in tool plug test tube, water-bath evaporates ethyl acetate, n-hexane is washed, is evaporated；Add
1ml water, 4ml anthrone reagents, boiling water bath 10min, frozen water cooling；620nm surveys OD values.
In the present embodiment after fermented 6D, the final pol of tunning is 0.3g/L, and sophorolipid content is 320g/L.
2nd, skin care articles for washing is prepared
A, in weighing each component under aseptic condition, be classified as three groups：The tunning of step 1 is A groups；White fungus slag charge,
Mixed under glycerine (food grade glycerin) and titanium dioxide (food-grade titanium dioxide) normal temperature or low temperature, be B groups；Methylchloroisothiazandnone
Mixed with methylisothiazolinone, be C groups, it is standby.
B, by A, B group composition 115 DEG C in autoclave, 0.07Mpa sterilizing 30min, it is stand-by；
C, in mixing A, B group composition under aseptic condition, and add the C group compositions with the filtering of 0.45 μm of sterilizing filter, it is low
The lower homogeneous of temperature is mixed, and produces the skin care articles for washing of the present embodiment.
A kind of skin care articles for washing of embodiment 2 and preparation method thereof
The skin care articles for washing of the present embodiment is mildy wash, includes the raw material of following parts by weight：White fungus slag charge tunning
55 parts, 36 parts of white fungus slag charge, 5 parts of glycerine, 4 parts of titanium dioxide, 0.0012 part of methylchloroisothiazandnone, methylisothiazolinone
0.0004 part.
The preparation method of the skin care articles for washing of the present embodiment, comprises the following steps：
1st, white fungus slag charge tunning is prepared
A, liquid seeds culture, domestication and expansion culture
Torulopsis inclined-plane seed that a ring activated is inoculated with super-clean bench (strain source in U.S. ATCC, by excellent
Change screening) in seed culture medium, 200rpm, 32 DEG C of culture 30h, seed culture medium is consisted of the following composition：Glucose 45g/
L, corn oil 30g/L, with 0.10% (w/v) KH2PO4And Na2HPO4Adjust pH to 6.3,121 DEG C of sterilizing 30min.
B, fermentation
Fermentation medium is prepared, fermentation medium is consisted of the following composition：Glucose 80g/L, corn oil 80g/L, finally
With white fungus slag charge and water constant volume, the wherein volume fraction of white fungus slag charge is 75%, with 0.10% (w/v) KH2PO4And Na2HPO4
PH to 6.2 is adjusted, is mixed using homogenizer, homogeneous.
It is added to after preparing in fermentation tank, 121 DEG C of sterilizing 30min are cooled to 32 DEG C.
Step a liquid seeds liquid is pumped into by 5% inoculum concentration, 32 DEG C ferment 6 days, are less than in pol (with glucose meter)
Terminate fermentation during 0.5g/L, collect tunning.
Sophorolipid assay method be the same as Example 1.
In the present embodiment after fermented 6D, the final pol of tunning is 0.3g/L, and sophorolipid content is 380g/L.
2nd, skin care articles for washing is prepared
A, in weighing each component under aseptic condition, be classified as three groups：The tunning of step 1 is A groups；White fungus slag charge,
Mixed under glycerine (food grade glycerin) and titanium dioxide (food-grade titanium dioxide) normal temperature or low temperature, be B groups；Methylchloroisothiazandnone
Mixed with methylisothiazolinone, be C groups, it is standby.
B, by A, B group composition 115 DEG C in autoclave, 0.07Mpa sterilizing 30min, it is stand-by；
C, in mixing A, B group composition under aseptic condition, and add the C group compositions with the filtering of 0.22 μm of sterilizing filter, nothing
Bacterium condition normal temperature homogeneous is mixed, and produces the skin care articles for washing of the present embodiment.
The mildy wash of the embodiment of the present invention 2 of comparative example 1 and the performance comparison of three kinds of commercially available mildy wash
1st, clean effect is tested
1) wash solution is prepared
Different articles for washing (mildy wash of the embodiment of the present invention 2 and the three kinds of cities of equivalent are first respectively put into beaker
The brand mildy wash sold), then stirred respectively toward the warm water of injection equivalent in beaker with glass bar.
2) it is put into soiled cotton
Cloth of the same area with equivalent edible oil and ink is separately immersed in this several wash solution, soaked
5 minutes.
3) soiled cotton is pulled out
After 5 minutes, cloth is taken out, the change of spot and the color of solution is conscientiously observed.
4) soiled cotton is washed by rubbing with the hands
In order to ensure that the cleaning of the spot on soiled cotton is cleaner and cleaning dynamics are the same, every group undertake the task of washing by rubbing with the hands must be all
It is schoolgirl or boy student.Every block of soiled cotton was washed by rubbing with the hands after 3 minutes, observation spot change is cleaned with water.
5) paired observation
Cloth after cleaning is contrasted, sees which is cleaner.After comparing, experimental result is filled in experimental record list
On.See which group is completed best.
2nd, irritation test
200 experimenters are picked out at random, the age was evenly distributed from 18 years old to 35 years old, directly using the embodiment of the present invention 2
Mildy wash and three kinds of commercially available brand mildy wash contact skins are tested.
3rd, it is biodegradable
Carried out by brand mildy wash commercially available to the mildy wash of the embodiment of the present invention 2 and three kinds standard GB/T/T15818
Test
4th, bio-toxicity
Mildy wash and three kinds of commercially available brands to the embodiment of the present invention 2 wash one's face milk material carry out analysis draw.
Above-mentioned performance comparison result such as table 1 below.
The mildy wash of the embodiment of the present invention 2 of table 1 and the performance comparison result of three kinds of commercially available brand mildy wash
| Product is contrasted | Clean effect | Excitant | 28 days biological degradation rates | Bio-toxicity |
| The present embodiment 2 | 73.63% | Nothing | 100% | Nothing |
| Commercially available mildy wash 1 | 66.37% | Nothing | 94% | Nothing |
| Commercially available mildy wash 2 | 71.90% | Minimal irritation | 92% | Nothing |
| Commercially available mildy wash 3 | 83.79% | Minimal irritation | 87% | Micro- poison |
It is apparent from by the result of table 1, the dairy products of washing one's face of the embodiment of the present invention 2 has non-stimulated, and what can all be degraded is excellent
Point, as skin care articles for washing, the clean effect of product of the present invention is also sufficient for using.
The tunning of the present invention of comparative example 2 and the contrast test of several conventional synthetic surfactants
Set two formulas that mildy wash is made, contrasted with the mildy wash of embodiment 2, formula is as follows respectively：
Contrast surfactant 1：35 parts of fatty acid potassium soap, 20 parts of laurel alcohol ether phosphate potassium, 36 parts of white fungus slag charge, glycerine
5 parts, 4 parts of titanium dioxide, 0.0012 part of methylchloroisothiazandnone, 0.0004 part of methylisothiazolinone.
Contrast surfactant 2：35 parts of lauryl Iminodiacetic acid sodium salt, dodecanamide propyl decil lactone 20
Part, 36 parts of white fungus slag charge, 5 parts of glycerine, 4 parts of titanium dioxide, 0.0012 part of methylchloroisothiazandnone, methylisothiazolinone
0.0004 part.
Making step is as follows：
A, each component is weighed, it is divided into two groups：Surfactant, white fungus slag charge, glycerine (food grade glycerin) and titanium dioxide
Mixed under (food-grade titanium dioxide) normal temperature or low temperature, be A groups；Methylchloroisothiazandnone and methylisothiazolinone are mixed, and are B
Group, it is standby.
B, by A group compositions 115 DEG C in autoclave, 0.07Mpa sterilizing 30min, it is stand-by；
C, the C group compositions filtered in aseptic condition addition with 0.22 μm of sterilizing filter, aseptic condition normal temperature homogeneous are mixed,
Produce corresponding articles for washing.
900 experimenters are picked out at random, the age was evenly distributed from 18 years old to 35 years old, be divided into three groups and use the present embodiment respectively
The mildy wash that 2 mildy wash is made with contrast surfactant 1 and contrast surfactant 2 carries out daily face cleaning test, experiment
As a result such as table 2.
The test result for the mildy wash that the embodiment of the present invention 2 of table 2 and contrast surfactant are made
As seen from the results in Table 2, common synthetic surfactant, which is used for a long time, to have damage to skin, cause moisture of skin not
Foot, satisfaction is high in actual applications for the product of the present embodiment 2, nonirritant, and not only face cleaning effect is good for long-term use, and does not damage
Hinder skin.
In addition, also carrying out total number of bacteria measure, the result such as table of experiment to the Product recycling after routine use 20 days
Shown in 3.
Table 3 uses the product bacterium total testing after 20 days
Drawn by the result of table 3, three kinds of products (≤100CFU) in the range of national regulation, are qualified products, but
The total number of bacteria and total number of molds of the mildy wash of the embodiment of the present invention 2 are below other comparative example products, and this also demonstrates this hair
Bright white fungus slag charge tunning has bacteriostasis.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of preparation method of white fungus slag charge tunning, it is characterised in that comprise the following steps：By 1~5% inoculation
In amount, the fermentation medium for pH5.5~6.5 that yeast starter liquid pump is entered to sterilizing, 25~32 DEG C ferment 5~8 days, in glucose
Pol terminates fermentation when being less than 0.5g/L, collect tunning, produce, the fermentation medium contains 40~100g/L grape
The white fungus slag charge of sugar, 30~80g/L corn oil and 40~75%v/v；The temperature of the fermentation medium is 25~32
℃。
2. the preparation method of white fungus slag charge tunning according to claim 1, it is characterised in that the yeast starter liquid
Preparation method be：The yeast inclined-plane seed activated is seeded in the seed culture medium of pH5.5~6.5 of sterilizing, 120~
200rpm, 25~32 DEG C of 24~40h of culture；The seed culture medium includes 20~50g/L glucose and 15~40g/L jade
Rice bran oil.
3. the white fungus slag charge tunning that the preparation method described in claim 1 or 2 is prepared.
4. application of the white fungus slag charge tunning in terms of skin care articles for washing described in claim 3.
5. a kind of skin care articles for washing, it is characterised in that it includes the raw material of following parts by weight：White fungus described in claim 3
45~67.5 parts of slag charge tunning, 22.5~45 parts of white fungus slag charge, 1~10 part of glycerine, 0~6.5 part of titanium dioxide.
6. skin care articles for washing according to claim 5, it is characterised in that it includes the raw material of following parts by weight：It is above-mentioned
50~60 parts of white fungus slag charge tunning, 25~35 parts of white fungus slag charge, 4~6 parts of glycerine, 2~4 parts of titanium dioxide.
7. the skin care articles for washing according to claim 5 or 6, it is characterised in that the skin care articles for washing also includes first
0.00027~0.0012 part of base chloroisothiazole quinoline ketone, 0.00009~0.0004 part of methylisothiazolinone, and the methyl chloride
The weight ratio of OIT and methylisothiazolinone is 3：1.
8. the skin care articles for washing according to claim 5 or 6, it is characterised in that the glycerine is food grade glycerin, medicinal
Level glycerine or 99.5% regular grade glycerine；The titanium dioxide is rutile titania, rutile or food-grade titanium dioxide.
9. a kind of preparation method of skin care articles for washing, it is characterised in that comprise the following steps：
(1), by white fungus slag charge tunning, white fungus slag charge, glycerine and titanium dioxide 115~121 DEG C in autoclave, 0.07~
15~45min of 0.11Mpa sterilizings；
(2), under aseptic condition, add through 0.22~0.45 μm of methylchloroisothiazandnone filtered and methylisothiazolinone,
Homogeneous is mixed, and is produced.
10. the preparation method of skin care articles for washing according to claim 9, it is characterised in that homogeneous described in step (2)
Mixing is aseptically carried out.
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| CN107177643B (en) | 2021-07-30 |
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