CN107177643B

CN107177643B - Tremella residue fermentation product, skin-care washing product containing fermentation product and preparation method of skin-care washing product

Info

Publication number: CN107177643B
Application number: CN201710527932.5A
Authority: CN
Inventors: 涂桂洪; 邓惠平; 潘忠林; 罗雄生; 谭志康
Original assignee: Guangzhou Meizhong Biotechnology Co ltd
Current assignee: Guangzhou Meizhong Biotechnology Co ltd
Priority date: 2017-06-30
Filing date: 2017-06-30
Publication date: 2021-07-30
Anticipated expiration: 2037-06-30
Also published as: CN107177643A

Abstract

The invention discloses a tremella residue fermentation product, a washing and skin-care product containing the tremella residue fermentation product and a preparation method of the tremella residue fermentation product. The biological surfactant sophorolipid in the white fungus residue fermentation product is used as one of the components of the washing and skin care product, and compared with the skin care product added with the chemically synthesized surfactant, the washing and skin care product has the advantages of lower toxicity, higher biodegradability, better environmental compatibility, higher foamability, better selectivity and specificity under extreme temperature, pH and salt concentration, higher skin affinity, no strong stimulation to the skin in the using process and safer product.

Description

Tremella residue fermentation product, skin-care washing product containing fermentation product and preparation method of skin-care washing product

Technical Field

The invention belongs to the technical field of applied biology, and particularly relates to a tremella residue fermentation product and a preparation method thereof, and a washing and skin-care product containing the tremella residue fermentation product and a preparation method thereof.

Background

The surfactant is an essential component in raw materials of washing skin care products, plays roles in wetting, emulsifying, foaming, thickening, solubilizing, decontaminating and the like, and is extremely widely applied in actual production.

Most of the surfactants added in the washing skin care products in the current market are artificially and chemically synthesized. The chemical surfactant can cause pollution to the environment to different degrees in actual production, such as change of the physical and chemical properties of soil, pollution to water bodies and the like, and is not easy to degrade. In use, certain chemical surfactants can cause irritation to the skin of the user, causing symptoms such as redness, inflammation, and irritation.

The biosurfactant is a metabolite with surface activity produced by microorganisms under certain conditions, and comprises glycolipid, polysaccharide lipid, lipopeptide or neutral lipid derivative and the like. Biosurfactants not only have various surface properties as do chemical surfactants, but also have superior properties that are not comparable to the latter: the applicability is wide; the biological toxicity is extremely low, and the method is environment-friendly; is suitable for extreme temperature, pH and salinity; the molecular structure is various, and part of the molecular structure has a plurality of special functional groups and has excellent surface performance.

Sophorolipid is the most promising biosurfactant in the development and utilization at the present stage, and one reason is that the yield and the conversion rate of sophorolipid are higher than those of other biosurfactants; another important reason is that the rhamnolipid-producing strain, the main competitor, is a pathogenic Pseudomonas aeruginosa strain, while the sophorolipid-producing strain is a non-pathogenic yeast strain. Sophorolipid as a biosurfactant belongs to a secondary metabolite of microorganisms, not only has common characteristics of the surfactants (such as wetting action, osmosis action, foaming action, solubilization action and the like), but also has unique physicochemical characteristics, and mainly comprises the following aspects:

first, sophorolipids have excellent surface activity. Sophorolipids belong to the group of non-ionic surfactants, the molecular structure of which comprises two groups: the hydrophilic group and the hydrophobic group are formed by long-chain saturated or unsaturated hydroxy fatty acid chains with 16-18 carbon atoms, and can be uniformly distributed and arranged into a layer in an aqueous solution, so that the surface tension is greatly reduced. There are related reports that the surface tension can be reduced to 30-40 mN/m when the concentration of sophorolipid in the aqueous solution is 40-100 mg/L. In addition, the sophorolipid has low critical micelle concentration and interfacial tension characteristic, and can change the surface property of the system easily after being added into a solution.

Second, sophorolipids have good chemical and thermal stability. It was reported that the emulsification ability and the surface tension-reducing property of sophorolipid were measured after 1 hour in a constant temperature water bath at 90 ℃ and found to be almost unchanged. In addition, the sophorolipid can maintain good characteristics even under high acid-base and high salt conditions.

Thirdly, sophorolipid is non-toxic and friendly to ecological environment. The sophorolipid belongs to a natural product of microbial fermentation, the synthesis process is the process of microbial metabolism and enzyme catalysis, the reaction condition is mild, no waste residue is generated, and the environment is hardly influenced. The sophorolipid has the characteristics of no toxicity, no odor and no irritation, and can be biodegraded and consumed after being finally circulated to the natural environment without polluting products.

Fourthly, the sophorolipid has a complex and rich molecular structure and has a certain inhibition effect on the saprogenic bacteria. Sophorolipids have more than 20 structural analogues, and a large number of novel sophorolipids have been discovered. It is shown by research that the sophorolipid with the concentration of 0.5g/L can almost completely inhibit the growth of yeast, and has 60 percent inhibition rate on mould. The inhibition rate of the sophorolipid with the concentration of 2.0g/L on the mould is over 80 percent. Meanwhile, the sophorolipid has good inhibition effect on mould hyphae and microorganisms in the air. 3.0g/L sophorolipid can inhibit more than 95% of microorganisms in the air, and the inhibition rate of hypha propagation is more than 70%; in addition, sophorolipid can inhibit and delay the spore production of hyphae.

Tremella is also called tremella, tremella etc., is a kind of edible fungus and medicinal fungus with high economic value and high value, and belongs to the traditional food and medicine dual-purpose product. The production area of tremella is very wide, and the tremella is mainly distributed in the areas of Sichuan, Zhejiang, Fujian, Jiangsu, Jiangxi, Anhui, Taiwan, Hubei, Hainan, Hunan, Guangdong, hong Kong, Guangxi, Guizhou, Yunnan, Shanxi, Gansu, inner Mongolia, Tibet and the like with suitable climate for growth. In industrial application, tremella is mainly used as a raw material to extract tremella polysaccharide, and the main method for extracting tremella polysaccharide at the present stage is a hot water extraction method; alkali extraction method; acid extraction; enzyme extraction method, etc., and the result of research shows that the polysaccharide yield is highest by hot water extraction method; and the hot water extraction method is optimized for multiple times of conditions, and the polysaccharide yield is only 16.81 percent. Therefore, the extracted tremella residue still contains a large amount of available substances, and has high utilization value. However, most enterprises generally do not develop and utilize the tremella slag again, and choose to directly discharge the tremella slag, which is a waste of resources to a certain extent.

In conclusion, the biosurfactant product has good performance, safety, environmental protection and wide application prospect, but the industrialization is still not mature, and the comprehensive production cost is higher. Therefore, most of the washing products on the market still use chemically synthesized surfactants, but the washing products added with biosurfactants are rare in types and expensive, and are not acceptable by general people. If the biosurfactant with various excellent characteristics can be produced by adopting the raw materials with low price and wide sources and processed into a biological washing product, the production cost can be greatly reduced, the enterprise benefit is improved, meanwhile, the industrial byproducts can be secondarily utilized, the resource utilization rate is greatly improved, and the strategic concept of energy conservation and emission reduction is really met.

Chinese patent CN 201310077379.1A preparation method of microbial detergent discloses a preparation method of a detergent containing biosurfactant: the method comprises the steps of producing rhamnolipid by using pseudomonas aeruginosa through fermentation, producing lipopeptide by using bacillus subtilis through fermentation, and mixing 5-15 parts of rhamnolipid extract, 0.01-0.05 part of lipopeptide extract, 0.01-0.05 part of 10000u/g lipase, 1-10 parts of sucrose fatty acid ester and 0.5-1.5 parts of EDTA to obtain the finished product of the microbial detergent. Wherein, the rhamnolipid and the lipopeptide are biological surfactants, the sucrose fatty acid ester is a chemically synthesized surfactant, the lipase decomposes fat to enhance the washing effect, and the EDTA is a chelating agent. The method adopts biosurfactant to replace the traditional chemically synthesized surfactant, well eliminates the adverse effect of the traditional surface activity on the environment, and has safe and mild biological surface activity and weak skin irritation. The production process adopts a biological fermentation method, has small influence on the environment, is easy to degrade the detergent, does not cause secondary pollution, and meets the requirements of the society on skin affinity, environmental protection and low carbon of the detergent. However, in the preparation method of the microbial detergent, the fermentation medium of the rhamnolipid consists of soybean oil, sunflower seed oil, yeast extract and salts, and certain defects still exist in the aspect of full utilization of resources. In addition, the rhamnolipid producing bacteria are pseudomonas aeruginosa with high pathogenicity, and if errors occur in a later purification process, fermentation products carry pathogenic bacteria, so that the risk of polluting a detergent exists, and serious harm is brought to a user.

Disclosure of Invention

Based on the above, in order to overcome the defects of the prior art, the invention provides a tremella residue fermentation product, a preparation method thereof and application thereof in the aspect of skin care washing products.

In order to realize the purpose of the invention, the invention adopts the following technical scheme:

a preparation method of a tremella residue fermentation product comprises the following steps: pumping the yeast seed solution into a sterilized fermentation medium with the pH value of 5.5-6.5 according to the inoculation amount of 1-5%, fermenting for 5-8 days at the temperature of 25-32 ℃, finishing fermentation when the sugar degree is less than 0.5g/L, and collecting fermentation products to obtain the tremella fuciformis berk, wherein the fermentation medium contains 40-100 g/L glucose, 30-80 g/L corn oil and 40-75% (v/v) tremella fuciformis residue; the temperature of the fermentation medium is 25-32 ℃; the sugar degree is measured in glucose.

In some embodiments, the yeast seed liquid is prepared by: inoculating the activated yeast slant seeds into a sterilized seed culture medium with the pH value of 5.5-6.5, and culturing at the temperature of 25-32 ℃ for 24-40 h at the rpm of 120-200; the seed culture medium comprises 20-50 g/L glucose and 15-40 g/L corn oil.

In some embodiments, the sterilization is carried out at 115-121 ℃ for 15-30 min.

The invention also provides a tremella residue fermentation product prepared by the preparation method.

The invention also provides application of the tremella residue fermentation product in the aspect of skin care washing products.

The skin-care washing product comprises the following raw materials in parts by weight: 45-67.5 parts of the tremella residue fermentation product, 22.5-45 parts of the tremella residue, 1-10 parts of glycerol and 0-6.5 parts of titanium dioxide.

In some embodiments, the skin care washing product comprises the following raw materials in parts by weight: 50-60 parts of the tremella residue fermentation product, 25-35 parts of tremella residue, 4-6 parts of glycerol and 2-4 parts of titanium dioxide.

In some embodiments, the skin care washing product further comprises 0.00027 to 0.0012 parts of methyl chloro isothiazolinone, 0.00009 to 0.0004 parts of methyl isothiazolinone, and the methyl chloro isothiazolinone and the methyl isothiazolinone are used as preservatives of the product.

In some of these embodiments, the weight ratio of methylchloroisothiazolinone to methylisothiazolinone is 3: 1.

in some embodiments, the glycerin is food grade glycerin, pharmaceutical grade glycerin, or 99.5% common grade glycerin, preferably food grade glycerin, and the glycerin plays a role in moistening and moisturizing.

In some embodiments, the titanium dioxide is anatase titanium dioxide, rutile titanium dioxide or food-grade titanium dioxide, and the titanium dioxide has high tinting strength and covering power and serves as a white pigment to achieve a whitening effect.

In some embodiments, the skin care washing product is a shampoo, a hand sanitizer, a body wash or a facial cleanser.

The invention also provides a preparation method of the skin-care washing product, which comprises the following steps:

(1) sterilizing the white fungus residue fermentation product, the white fungus residue, glycerol and titanium dioxide in a sterilization pot at 115-121 ℃ and 0.07-0.11 Mpa for 15-45 min;

(2) and under the aseptic condition, adding the methyl chloroisothiazolinone and the methyl isothiazolinone which are filtered by 0.22-0.45 mu m, and homogenizing and uniformly mixing to obtain the composition.

In some of the embodiments, the sterilization time in the step (1) is 20-35 min.

In some of these embodiments, the homogenizing in step (2) is performed under aseptic conditions.

The invention utilizes the byproduct of tremella after extracting polysaccharide, namely tremella slag to carry out biological fermentation to obtain a fermentation product containing biosurfactant sophorolipid, and then a biological fermentation skin-care washing product which is natural, efficient and mild in action is processed and prepared, compared with the prior art, the invention has the following beneficial effects:

1. the raw materials of the fermentation product of the invention are from industrial waste tremella material residues, and tremella still has a large amount of utilizable substances after polysaccharide is extracted, so that the industrial waste is recovered and developed and utilized, thereby saving the production cost, fully developing and utilizing resources, reducing the discharge amount of industrial waste, improving the utilization rate of the raw materials, and conforming to the strategic concept of sustainable development of energy conservation and emission reduction;

2. during the fermentation process, various enzymes, amino acids, vitamins, organic acids and polypeptide substances are generated in the microbial metabolism process, and the enzyme substances have good oil fat removing and cutin removing capabilities and can act synergistically with sophorolipid; the substances such as amino acid, vitamin, polypeptide and the like contained in the skin care lotion can clean the skin and have the effects of nursing and nourishing;

3. the sophorolipid is produced by microbial fermentation by taking pure natural and edible white fungus residues as main raw materials, has lower toxicity, higher biodegradability, better environmental compatibility and higher foamability, has better selectivity and specificity under extreme temperature, pH and salt concentration, and has performance superior to that of a chemically synthesized surfactant; moreover, the sophorolipid has higher skin affinity, does not generate strong stimulation to the skin in the using process and has more advantages in product popularization; the bacteria for producing the sophorolipid is the non-pathogenic microzyme, has no high pathogenicity like pseudomonas aeruginosa for producing the rhamnolipid, and in addition, the washing and skin-care product does not contain components with toxic and side effects on human bodies, such as bleaching agent, fluorescent whitening agent, artificial essence, pigment and the like, and the characteristics ensure that the product is safer.

Detailed Description

The invention will be further described with reference to specific examples, which are not described herein as being applicable to the prior art. Specific examples of the present invention are given below, but the examples are only for the purpose of further elaborating the present invention and do not limit the claims of the present invention. The reagents and starting materials used in the following examples were all commercially available unless otherwise specified.

The terms involved in the present invention are explained as follows:

fermentation: refers to a process for preparing microbial cells themselves or microbial metabolites by virtue of the life activities of microorganisms under aerobic or anaerobic conditions.

Biosurfactant: the surface active substances are generated by microbial fermentation, and can play roles in thickening, solubilization, emulsification, foaming and the like when added into cosmetics.

Domesticating strains: refers to a method for directionally breeding microorganisms by gradually adapting the microorganisms to a certain condition through artificial measures. Strains with higher tolerance and activity can be obtained by domestication.

Culture medium: artificially formulated nutrients for the growth and maintenance of microorganisms, plant tissues and animal tissues generally contain carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins and water.

White fungus slag charge: the white fungus is taken as a residue after polysaccharide extraction.

The Torulopsis glabrata slant seed strain used in the following examples is derived from American ATCC, and is obtained by conventional optimization screening with white fungus slag as a basic culture medium.

EXAMPLE 1 skin-protecting washing product and method for preparing the same

The skin-care washing product of the embodiment is shampoo and comprises the following raw materials in parts by weight: 45 parts of tremella residue fermentation product, 45 parts of tremella residue, 5 parts of glycerol, 0.00027 part of methyl chloroisothiazolinone and 0.00009 part of methyl isothiazolinone.

The preparation method of the skin care washing product comprises the following steps:

1. preparation of Tremella residue fermentation product

a. Liquid seed culture, acclimatization and amplification culture

Inoculating a ring of activated Torulopsis glabrata slant seeds in a super clean bench, and culturing for 24h at 180rpm and 30 ℃, wherein the seed culture medium consists of the following components: glucose 30g/L, corn oil 25g/L, with KH of 0.10% (w/v)₂PO₄And Na₂HPO₄Adjusting pH to 6.0, and sterilizing at 115 deg.C for 20 min.

b. Fermentation of

Preparing a fermentation medium, wherein the fermentation medium comprises the following components: 60g/L glucose and 50g/L corn oil, and finally fixing the volume by using tremella slag and water, wherein the volume fraction of the tremella slag is 75 percent, and 0.10 percent (w/v) KH is used₂PO₄And Na₂HPO₄Adjusting pH to 6.0, mixing with homogenizer, and homogenizing.

Adding into fermentation tank, sterilizing at 115 deg.C for 30min, and cooling to 30 deg.C.

Pumping the liquid seed liquid of the step a according to the inoculation amount of 2 percent, fermenting for 6 days at the temperature of 30 ℃, finishing the fermentation when the sugar degree (calculated by glucose) is less than 0.5g/L, and collecting the fermentation product.

The sophorolipid in the fermentation product is determined by adopting an anthrone method, and the principle of determining the total sugar is as follows: the anthrone reaction is a sugar determination method that can be used to determine hexoses, aldopentoses, and hexuronic acids, and all sugars exhibit a blue-green color, whether in free form or in the presence of polysaccharides. At 620nm, maximum light absorption is exhibited. The method is rapid and convenient. The method comprises the following specific steps:

accurately weighing 0.1g of anthrone reagent, adding 100ml of concentrated sulfuric acid, and uniformly mixing to obtain the anthrone reagent.

Preparing 10mg/ml of anhydrous glucose solution: accurately weighing 0.1g of analytically pure anhydrous glucose (dried at 105 ℃ to constant weight in advance), dissolving with distilled water, transferring into a 100ml volumetric flask, washing the volumetric flask with distilled water for several times to transfer all glucose into the volumetric flask, finally fixing the volume to a scale, shaking up, and obtaining the concentration of 1000 mg/L. Then 0.5, 1, 2, 3 and 4ml are respectively taken to be constant volume to 100ml, and the concentration is respectively 5, 10, 20, 30 and 40 mg/L.

Drawing a glucose standard curve by an anthrone method: measuring 4ml of anthrone reagent and 1ml of standard glucose solution, placing in a test tube with a plug, rapidly soaking in ice bath for cooling, heating in boiling water bath for 10min, cooling in ice bath, measuring OD value with spectrophotometer at 620nm (1ml of distilled water is blank control).

Sophorolipid assay principle: the sophorolipid is composed of sophorose and lipid, OD value of sophorolipid sample at 620nm is determined by anthrone method, and glucose content is determined from glucose standard curve determined by anthrone method according to OD value; the sophorolipid content of the sample was determined by the ratio between the sophorolipid and the glucose molecular weight, i.e. 1g glucose corresponds to 1.91g sophorolipid.

And (3) sophorolipid determination: taking 1ml of fermentation product, adding 1ml of ethyl acetate with the same volume, uniformly mixing, standing for 5min, and centrifuging at 5000rpm for 5 min; adding 10ul of the mixture into a test tube with a plug, evaporating ethyl acetate in a water bath, washing with n-hexane, and evaporating to dryness; adding 1ml of water and 4ml of anthrone reagent, boiling in a water bath for 10min, and cooling with ice water; OD was measured at 620 nm.

After fermentation for 6D in this example, the final sugar degree of the fermentation product was 0.3g/L and the sophorolipid content was 320 g/L.

2. Preparing skin-care washing product

a. Weighing the components under aseptic condition, and dividing the components into three groups: the fermentation product of the step 1 is group A; mixing Tremella residue, glycerol (food grade glycerol) and titanium dioxide (food grade titanium dioxide) at room temperature or low temperature to obtain group B; and uniformly mixing the methyl chloroisothiazolinone and the methyl isothiazolinone to form a group C for later use.

b. Sterilizing A, B components in a sterilizing pot at 115 deg.C and 0.07Mpa for 30 min;

c. mixing A, B components under aseptic condition, adding component C filtered by 0.45 μm aseptic filter, homogenizing at low temperature, and mixing to obtain the skin care washing product.

Example 2A skin-care detergent and method for preparing the same

The skin-care washing product of the embodiment is facial cleanser and comprises the following raw materials in parts by weight: 55 parts of tremella residue fermentation product, 36 parts of tremella residue, 5 parts of glycerol, 4 parts of titanium dioxide, 0.0012 part of methyl chloro isothiazolinone and 0.0004 part of methyl isothiazolinone.

1. preparation of Tremella residue fermentation product

a. Liquid seed culture, acclimatization and amplification culture

Inoculating a ring of activated Torulopsis glabrata slant seeds (the strains are from American ATCC and are subjected to optimized screening) in a super clean bench, and culturing for 30h at 200rpm and 32 ℃, wherein the seed culture medium consists of the following components: 45g/L glucose, 30g/L corn oil, 0.10% (w/v) KH₂PO₄And Na₂HPO₄Adjusting pH to 6.3, and sterilizing at 121 deg.C for 30 min.

b. Fermentation of

Preparing a fermentation medium, wherein the fermentation medium comprises the following components: 80g/L glucose and 80g/L corn oil, and finally fixing the volume by using tremella slag and water, wherein the volume fraction of the tremella slag is 75 percent, and 0.10 percent (w/v) KH is used₂PO₄And Na₂HPO₄Adjusting pH to 6.2, mixing with homogenizer, and homogenizing.

Adding into a fermentation tank after preparation, sterilizing at 121 deg.C for 30min, and cooling to 32 deg.C.

Pumping the liquid seed liquid of the step a according to the inoculation amount of 5 percent, fermenting for 6 days at 32 ℃, ending the fermentation when the sugar degree (calculated by glucose) is less than 0.5g/L, and collecting the fermentation product.

Sophorolipid assay procedure was the same as in example 1.

After fermentation for 6D in this example, the final sugar degree of the fermentation product was 0.3g/L and the sophorolipid content was 380 g/L.

2. Preparing skin-care washing product

c. a, B components are mixed under aseptic condition, and C component filtered by a 0.22 μm aseptic filter is added, and the skin care washing product of the embodiment is obtained by homogenizing and mixing evenly under aseptic condition at normal temperature.

Comparative example 1 comparison of the performance of the facial cleanser of example 2 of the present invention with three commercially available facial cleansers

1. Test of soil Release Effect

1) Preparing a detergent solution

Different washing products (the facial cleanser of the embodiment 2 of the invention and three commercially available brands of facial cleansers) with the same amount are respectively put into beakers, warm water with the same amount is respectively injected into the beakers, and the beakers are uniformly stirred by a glass rod.

2) Put in dirty cloth

The same area of cloth strips with equal amounts of edible oil and ink were soaked in these washing solutions for 5 minutes, respectively.

3) Dirty cloth is fished out

After 5 minutes, the cloth strip was removed and the stain change and solution color were carefully observed.

4) Scrubbing dirty cloth

In order to ensure that stains on the dirty cloth are cleaned more cleanly and the cleaning force is the same, all groups which undertake the scrubbing task must be girls or boys. After each piece of soiled cloth was rubbed for 3 minutes, the change in soil was observed by washing with water.

5) Comparative observations

And comparing the washed cloth strips to see which cloth strip is cleaner. After comparison, the experimental results are filled in the experimental record sheet. See which group completed the best.

2. Irritation test

200 panelists were randomly selected, evenly distributed from 18 to 35 years of age, and tested by direct skin contact using the facial cleanser of example 2 of the present invention and three commercially available brands of facial cleansers.

3. Biodegradation

The facial cleanser of example 2 of the invention and three commercially available brands were tested according to the national standard GB/T15818

4. Biotoxicity

The facial cleanser of example 2 of the invention and three commercially available brands of facial cleanser materials were analyzed.

The results of the above comparison of properties are shown in Table 1 below.

Table 1 comparison of the performance of the facial cleanser of example 2 of the present invention and three commercially available brands of facial cleansers

Product comparison	Decontamination effect	Irritation property	Biological degradation rate of 28 days	Biotoxicity
					Example 2	73.63％	Is free of	100％	Is free of
Commercial facial cleanser 1	66.37％	Is free of	94％	Is free of
					Commercial facial cleanser 2	71.90％	Slight stimulation	92％	Is free of
Commercial facial cleanser 3	83.79％	Slight stimulation	87％	Slight toxicity

As is apparent from the results in Table 1, the cleansing milk product of example 2 of the present invention has the advantages of no irritation and complete degradation, and the cleansing effect of the product of the present invention is sufficient for use as a skin-care cleansing product.

Comparative example 2 comparative testing of fermentation products of the invention and several commonly used synthetic surfactants

Two formulas are set to prepare the facial cleanser, and compared with the facial cleanser in example 2, the formulas are respectively as follows:

comparative surfactant 1: 35 parts of fatty acid potassium soap, 20 parts of lauryl alcohol ether phosphate potassium, 36 parts of tremella residue, 5 parts of glycerol, 4 parts of titanium dioxide, 0.0012 part of methyl chloroisothiazolinone and 0.0004 part of methyl isothiazolinone.

Comparative surfactant 2: 35 parts of disodium lauryl iminodiacetate, 20 parts of lauramidopropyl dimethylamine caprolactone, 36 parts of white fungus slag, 5 parts of glycerol, 4 parts of titanium dioxide, 0.0012 part of methyl chloroisothiazolinone and 0.0004 part of methyl isothiazolinone.

The preparation method comprises the following steps:

a. weighing the components, and dividing the components into two groups: mixing surfactant, Tremella residue, glycerol (food grade glycerol) and titanium white powder (food grade titanium white powder) at room temperature or low temperature to obtain group A; and uniformly mixing the methyl chloroisothiazolinone and the methyl isothiazolinone to form a group B for later use.

b. Sterilizing the component A in a sterilizing pot at 115 deg.C and 0.07Mpa for 30 min;

c. adding the component C filtered by a 0.22 μm sterile filter under sterile condition, homogenizing and mixing at room temperature under sterile condition to obtain corresponding washing product.

900 test subjects were randomly selected and evenly distributed from 18 to 35 years of age, and daily cleansing tests were carried out in three groups using the cleansing milk of example 2 and the cleansing milks prepared using comparative surfactant 1 and comparative surfactant 2, respectively, and the results of the tests are shown in Table 2.

Table 2 test results for facial cleanser made of example 2 of the invention and comparative surfactants

As can be seen from the results in Table 2, the common synthetic surfactant can damage the skin after long-term use, which results in insufficient moisture in the skin, and the product of the embodiment 2 has high satisfaction degree in practical application and no irritation, and not only has good face cleaning effect after long-term use, but also does not damage the skin.

In addition, the total number of bacteria was also determined for product recovery after 20 days of daily use, and the results of the experiment are shown in Table 3.

Table 3 total bacteria count test of the product after 20 days of use

The results in table 3 show that all three products are within the range (less than or equal to 100CFU) specified by the state and are qualified products, but the total number of bacteria and the total number of molds of the facial cleanser in the embodiment 2 are lower than those of the other comparative products, and the result also proves that the tremella residue fermentation product has the bacteriostatic action.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The skin-care washing product is characterized by comprising the following raw materials in parts by weight: 45-67.5 parts of tremella residue fermentation product, 22.5-45 parts of tremella residue, 1-10 parts of glycerol, 0-6.5 parts of titanium dioxide, 0.00027-0.0012 parts of methyl chloroisothiazolinone and 0.00009-0.0004 parts of methyl isothiazolinone, wherein the weight ratio of the methyl chloroisothiazolinone to the methyl isothiazolinone is 3: 1;

the preparation method of the tremella residue fermentation product comprises the following steps: pumping the yeast seed liquid into a sterilized fermentation medium with the pH value of 5.5-6.5 according to the inoculation amount of 1-5%, fermenting for 5-8 days at the temperature of 25-32 ℃, finishing fermentation when the glucose degree is less than 0.5g/L, and collecting a fermentation product to obtain the fermentation product containing the biosurfactant sophorolipid, wherein the fermentation medium comprises the following components: 40-100 g/L glucose, 30-80 g/L corn oil and 40-75% v/v white fungus residue, and finally, adding water to a constant volume, and using 0.10% (w/v) KH₂PO₄And Na₂HPO₄Adjusting the pH value; the temperature of the fermentation medium is 25-32 ℃, and the tremella residue is obtained by extracting polysaccharide from tremella;

the yeast is Torulopsis;

the preparation method of the yeast seed liquid comprises the following steps: inoculating the activated yeast slant seeds into a sterilized seed culture medium with the pH value of 5.5-6.5, and culturing at the temperature of 25-32 ℃ for 24-40 h at the rpm of 120-200; the seed culture medium comprises 20-50 g/L glucose and 15-40 g/L corn oil;

the preparation method of the skin-care washing product comprises the following steps:

(2) under the aseptic condition, adding the methyl chloroisothiazolinone and the methyl isothiazolinone which are filtered by 0.22-0.45 mu m, and homogenizing and uniformly mixing to obtain the product.

2. The skin-care washing product as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 50-60 parts of the tremella residue fermentation product, 25-35 parts of tremella residue, 4-6 parts of glycerol and 2-4 parts of titanium dioxide.

3. The skin care cleansing article of claim 2 wherein said glycerin is food grade glycerin, pharmaceutical grade glycerin, or 99.5% regular grade glycerin; the titanium dioxide is anatase titanium, rutile titanium or food-grade titanium dioxide.

4. The skin care cleansing product of claim 1 wherein said homogenizing in step (2) is carried out under aseptic conditions.