CN109674845A - A kind of methods and applications preparing Fermented Soybean using composite bacteria fermentation - Google Patents
A kind of methods and applications preparing Fermented Soybean using composite bacteria fermentation Download PDFInfo
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Abstract
A kind of methods and applications preparing Fermented Soybean using composite bacteria fermentation, it belongs to Chinese medicine preparation technical field, the purpose of the present invention is to solve current traditional Fermented Soybean concocting methods, toxic, harmful microorganism may be brought into, Fermented Soybean is made to influence safety and validity that it is used there are pathogenic bacteria pollution risk；And the problem that Fermented Soybean color is bad.And a kind of the problem of currently without preparation treatment stomatocace medicine using Fermented Soybean.Fermented Soybean of the invention is used to prepare the drug for the treatment of canker sore, the preparation method comprises the following steps: being mixed after soybean germination is handled with sweet wormwood and mulberry leaf decoction liquor, boiling after addition green alum, inoculate the mixed liquor fermentation of bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus, after drying, Fermented Soybean is obtained.The present invention is applied to Fermented Soybean processing and application field.
Description
Technical field
The invention belongs to Chinese medicine preparation technical fields, prepare light beans using composite bacteria fermentation in particular to a kind of
The method of fermented soya beans, salted or other wise.
Background technique
Fermented Soybean is that legume soybean Glycine max (L.) Merr. mature seed adds with sweet wormwood, mulberry leaf co-fermentation
Work forms, and is the kind that " Chinese Pharmacopoeia " records always from version in 1963 to version in 2015, has inducing diaphoresis relieving restlessness, what a surname sent out hot and suffocating
Effect, for catching a cold, fever and chills headache, irritated uncomfortable in chest, restlessness of asrhenia type and insomnia.Modern study confirms that Fermented Soybean contains isoflavones, oligomeric
A variety of physiologically active ingredients such as sugar, soyasaponin, Fibrinolytic Enzyme in Douchi, γ-aminobutyric acid have reduction by three high, anti-oxidant, anti-
Cancer, thrombus and estrogen-like effect, it is comprehensive to cardiovascular disease, diabetes, osteoporosis, breast cancer and Menopause
The prevention and control of the diseases such as simulator sickness have preferable effect.
Since the production method recorded in the concocted specification of Fermented Soybean various regions is had nothing in common with each other, there are a medicine number methods, each method in various regions
Or the phenomenon that method homogeneity is different so that Fermented Soybean attenuation degree available on the market is different, keep Fermented Soybean quality uncontrollable to
Directly affect clinical efficacy.Currently, the preparation process of Fermented Soybean still uses traditional natural fermentation process, fermenting microbe, fermentation temperature
The technological parameters such as degree, fermentation time are not known in pharmacopeia, due to various regions environment, weather, fermentation process difference and cause light
Fermented soya bean unstable quality and there is the problems such as mixing of poor quality, final product quality is irregular, it is of poor quality mix, color is bad etc., especially
It is that may bring toxic, harmful microorganism in natural fermentation process into, makes Fermented Soybean there are pathogenic bacteria pollution risk, influencing it makes
Safety and validity；The traditional zymotic period is long, although can be shortened the period using strain fermentation, in terms of enzyme activity not
Correlation further investigation, it is less that compound strain fermentation prepares Fermented Soybean report.Moreover, with the analysis of effective component to Fermented Soybean by
Gradually deeply, it is applied to range and is also gradually expanded, Fermented Soybean has new effect, it is also necessary to scientific and technical personnel's further investigation.
Summary of the invention
The purpose of the present invention is to solve current traditional Fermented Soybean concocting methods, may bring toxic, harmful micro- life into
Object makes Fermented Soybean influence safety and validity that it is used there are pathogenic bacteria pollution risk；And Fermented Soybean color is bad
Problem.And a kind of the problem of currently without preparation treatment stomatocace medicine using Fermented Soybean.And it provides a kind of using multiple
Close the method that strain fermentation prepares Fermented Soybean, and the application using Fermented Soybean preparation treatment stomatocace medicine.
A kind of method preparing Fermented Soybean using composite bacteria fermentation of the invention, it is followed the steps below:
Step 1: the water that 2~3 times of weight are added impregnates 12~16h after taking soybean to be cleaned with clear water, drains, be protected from light and put
It sets, carries out 24~48h of germination treatment；
Step 2: adding water to decoct jointly 2 times, each 1h into sweet wormwood and mulberry leaf, merging filtrate, concentration is for use；
Step 3: in the soybean concentrate of step 2 being added to after step 1 germination treatment, and be added and account for soybean weight
The green alum of amount 0.02% carries out 10~14h of immersion；Sterilize 30~40min of boiling under conditions of 121 DEG C, 0.105Mpa；It takes out
It is cooling to be placed in room temperature, obtains fermentation substrate；
Step 4: aseptically, fermentation substrate after taking step 3 boiling according to mass volume ratio be 100g:1~
The ratio of 2mL is inoculated with the mixed liquor of bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus, trains prior to 25~28 DEG C
7d is supported, then at 40~45 DEG C of culture 3d, then 50 DEG C or less dryings are to get Fermented Soybean；
Wherein, the mass ratio 100g:7-10g:7-10g of soybean, sweet wormwood and mulberry leaf.
Fermented Soybean of the invention is used to prepare the drug for the treatment of canker sore；The drug is by Fermented Soybean grind into powder system
At.
Fermented Soybean of the invention is used to prepare the drug for the treatment of canker sore；The drug of the treatment canker sore is by weight
Amount number be by 10~12 parts of cape jasmine, 25~35 parts of Fructus Forsythiae, 25~35 parts of the root bark of white mulberry, 10~15 parts of Radix Codonopsis, 10~
15 parts of Radix Astragali, 25~35 parts of Divine Comedy, 25~35 parts of hawthorn, 45~55 parts of malt, 10~15 parts of Fructus Aurantii, 10~15
Part rhizoma atractylodis, 10~12 parts in one's early teens, 25~35 parts of Fermented Soybean, 25~35 parts of caragana and 8~10 parts of Radix Glycyrrhizae group
At.
Fermented Soybean of the invention is used to prepare the drug for the treatment of canker sore；The drug of the treatment canker sore is divided into
Medicine for oral administration and externally applied drug；The externally applied drug is Fermented soybean；Medicine for oral administration be in parts by weight by 10~15 parts Radix Codonopsis, 10
~15 parts of Poria cocos, 10~15 parts of Rhizoma Atractylodis Macrocephalae, 8~10 parts of fennel seeds, 10~15 parts of Chinese yam, 10~15 parts of hawthorn, 10
~15 parts of Divine Comedy, 10~15 parts of Fermented Soybean, 10~12 parts of Cortex Phellodendri and 4~6 parts of Radix Glycyrrhizae composition.
Fermented Soybean of the invention is used to prepare the drug for the treatment of canker sore；The drug of the treatment canker sore is divided into
Medicine for oral administration and externally applied drug；The externally applied drug is Fermented soybean；Medicine for oral administration is in the walnut by 30~50 parts in parts by weight
Shell and 30~50 parts of glossy privet leaf are made.
The present invention include it is following the utility model has the advantages that
The research of the invention finds that Fermented Soybean is ground into end, after being applied with ulcer surface part, can allow canker sore (especially
It is recurrent oral ulceration) quickly improve, it especially uses, is applied in part before sleeping, pain will be clearly better after the evening,
Several days ulcer is mostly used almost to fully recover.If assisting the oral administration medicament prepared with Fermented Soybean, canker sore can be cured.It can be said that light
Fermented soya bean are new discoveries of the invention using treatment canker sore separately as drug.The drug for the treatment of canker sore of the invention can
Think Fermented Soybean externally applied drug, medicine for oral administration can also be combined and be used together, better effect.
The endo-medicine of Fermented Soybean preparation, side solve are as follows: canker sore is humidifier fever, and therefore, the present invention is with cape jasmine, Sang Bai
For skin as relieving superficies by cooling drug, Fructus Aurantii, rhizoma atractylodis, in one's early teens strengthening the spleen and stomach are aid digestion, remove eliminating dampness, canker sore prolonged illness and it is empty, because
This, with Fermented Soybean, in one's early teens, caragana qi-restoratives, build up health；Deficiency of vital energy person assists with Radix Codonopsis, Radix Astragali；Fermented soya bean, smell bitter cold, enter
Lung stomach two pass through, be apt to exploitation the part of the body cavity above the diaphragm housing the heart and lungs it is hot and suffocating, preferably let out yin it is turbid keep, pharmacological action with treat canker sore quite close, Fermented Soybean
Substances, the effect such as protein rich in, fat, carbohydrate, vitamin B complex, Yan acid and calcium, iron, microcosmic salt are not only conducive to burst
The stage of attack of ulcer, moreover it is possible to effectively prevent recurrence.
Treatment canker sore for a period of time after, although ulcer symptoms mitigate, it is multiple that spleen deficiency receives fortune, should reconcile spleen
Stomach；Therefore using for oral administration of Radix Codonopsis, Poria cocos, Rhizoma Atractylodis Macrocephalae, fennel seeds, Chinese yam, hawthorn, Divine Comedy, Fermented Soybean, Cortex Phellodendri and Radix Glycyrrhizae composition
Medicine.
And the medicine for oral administration of walnut inner casing and glossy privet leaf, make tea-drinking after decoction, even served 1~2 week, and Fermented Soybean is cooperated to be applied to
Oral ulcer affected part, canker sore can be effectively treated.
Fermented Soybean protein rich in, fat, carbohydrate, vitamin B complex, Yan acid and the substances such as calcium, iron, microcosmic salt, this
Invention it has been investigated that, obtained through bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus composite fermentation it is light
Fermented soya bean, protease, fibrinolysin and activity of beta-glucosidase with higher, can promote soybean protein fully degraded be small peptide and
Amino acid, improves the conversion ratio of plasmin activity and isoflavones methods of glycosides, and then improves its bioavilability.
Umbrella branch pears head mold fermentation generate glucuroide the isoflavone glycoside in soybean can be carried out decompose to
The Aglycons that can be absorbed by human small intestine are converted to, the soyabean oligosaccharides of generation can have improvement intestinal environment, drop
Blood pressure and cholesterol and strengthen immunity effect.Lactobacillus bulgaricus can be effectively improved taste.
Soybean after germination treatment, especially semen sojae atricolor, after germination treatment, semen sojae atricolor can generate more nitrogen phosphorus substances, have
It is absorbed and utilized conducive to umbrella branch pears head mould, and is formed by gap between semen sojae atricolor, be more preferably conducive to strain fermentation.
The present invention can be improved the brightness of Fermented Soybean by the way that green alum is added, and enhance its quality；Make its appearance more preferably.
Using composite bacteria, aseptically fermentation prepares Fermented Soybean to the present invention, can effectively avoid the pollution of harmful bacteria,
It is highly-safe.
The present invention first cultivates 7d using 25-28 DEG C, and the zymotechnique of 3d is cultivated then at 40-42 DEG C, specifies the hair of Fermented Soybean
Ferment technique parameter improves active constituent content, bioactivity is made to reach best.
Through the obtained Fermented Soybean of present invention processing, urokinase in 20~100IU/mL, plasmin activity up to 2204IU/g,
Aglycon total amount is up to 1335.23 μ g/g；Moreover, not containing detection of Salmonella, large intestine angstrom alkene bacterium, bile tolerance Gram-negative in Fermented Soybean
The pathogenic bacteria such as bacterium.
Specific embodiment
Specific embodiment 1: a kind of method for preparing Fermented Soybean using composite bacteria fermentation of present embodiment, it is
It follows the steps below:
Step 1: the water that 2~3 times of weight are added impregnates 12~16h after taking soybean to be cleaned with clear water, drains, be protected from light and put
It sets, carries out 24~48h of germination treatment；
Step 2: adding water to decoct jointly 2 times, each 1h into sweet wormwood and mulberry leaf, merging filtrate, concentration is for use；
Step 3: in the soybean concentrate of step 2 being added to after step 1 germination treatment, and be added and account for soybean weight
The green alum of amount 0.02% carries out 10~14h of immersion；Sterilize 30~40min of boiling under conditions of 121 DEG C, 0.105Mpa；It takes out
It is cooling to be placed in room temperature, obtains fermentation substrate；
Step 4: aseptically, fermentation substrate after taking step 3 boiling according to mass volume ratio be 100g:1~
The ratio of 2mL is inoculated with the mixed liquor of bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus, trains prior to 25~28 DEG C
7d is supported, then at 40~45 DEG C of culture 3d, then 50 DEG C or less dryings are to get Fermented Soybean；
Wherein, the mass ratio 100g:7-10g:7-10g of soybean, sweet wormwood and mulberry leaf.
Specific embodiment 2: the present embodiment is different from the first embodiment in that: soybean is semen sojae atricolor or soya bean.Its
It is same as the specific embodiment one.
Specific embodiment 3: the present embodiment is different from the first embodiment in that: amount of water is blueness in step 2
18 times of weight of wormwood artemisia and mulberry leaf.It is other same as the specific embodiment one.
Specific embodiment 4: the present embodiment is different from the first embodiment in that: the filtrate concentration in step 2
It is 1.10~1.12g/cm to relative density3.It is other same as the specific embodiment one.
Specific embodiment 5: the present embodiment is different from the first embodiment in that: the bacillus subtilis,
The mould lactobacillus bulgaricus strain of umbrella branch pears head is isolated from Fermented Soybean medicine materical crude slice；Wherein, bacillus subtilis is (Bacillus
Subtilis subsp.subtilis), it is preserved in Chinese industrial Microbiological Culture Collection administrative center, deposit number are as follows: CICC
24713.It is other same as the specific embodiment one.
Specific embodiment 6: the present embodiment is different from the first embodiment in that: the bacillus subtilis
Concentration with umbrella branch pears head mould mixed liquor is 107~109CFU/mL.It is other same as the specific embodiment one.
Specific embodiment 7: the present embodiment is different from the first embodiment in that: bacillus subtilis, umbrella branch pears
The mass ratio of head mould and lactobacillus bulgaricus is 3~4:2~3:1.It is other same as the specific embodiment one.
Specific embodiment 8: the application for the Fermented Soybean that present embodiment is prepared using specific embodiment one, it is used for
The drug of preparation treatment canker sore；The drug is made of by Fermented Soybean grind into powder.
Specific embodiment 9: the application for the Fermented Soybean that present embodiment is prepared using specific embodiment one, it is used for
The drug of preparation treatment canker sore；The drug of the treatment canker sore be in parts by weight by 10~12 parts cape jasmine,
25~35 parts of Fructus Forsythiae, 25~35 parts of the root bark of white mulberry, 10~15 parts of Radix Codonopsis, 10~15 parts of Radix Astragali, 25~35 parts of Divine Comedy,
25~35 parts of hawthorn, 45~55 parts of malt, 10~15 parts of Fructus Aurantii, 10~15 parts of rhizoma atractylodis, 10~12 parts in one's early teens,
25~35 parts of Fermented Soybean, 25~35 parts of caragana and 8~10 parts of Radix Glycyrrhizae composition.
Present embodiment treatment canker sore is to be used together the joint medicine for oral administration of the Fermented Soybean as externally applied drug, inside and outside medicine
It applies altogether, effectively healing canker sore.
Specific embodiment 10: the application for the Fermented Soybean that present embodiment is prepared using specific embodiment one, it is used for
The drug of preparation treatment canker sore；The drug of the treatment canker sore is divided into medicine for oral administration and externally applied drug；The external application
Medicine is Fermented soybean；Medicine for oral administration is by 10~15 parts of Radix Codonopsis, 10~15 parts of Poria cocos, 10~15 parts white in parts by weight
Art, 8~10 parts of fennel seeds, 10~15 parts of Chinese yam, 10~15 parts of hawthorn, 10~15 parts of Divine Comedy, 10~15 parts light
Fermented soya bean, 10~12 parts of Cortex Phellodendri and 4~6 parts of Radix Glycyrrhizae composition.
Present embodiment treatment canker sore is to be used together the joint medicine for oral administration of the Fermented Soybean as externally applied drug, medicine for oral administration
Can regulating spleen and stomach, fundamentally anti-treating dental ulcer.
Specific embodiment 11: the application for the Fermented Soybean that present embodiment is prepared using specific embodiment one, it is used
In the drug of preparation treatment canker sore；The drug of the treatment canker sore is divided into medicine for oral administration and externally applied drug；Described is outer
Medication is Fermented soybean；Medicine for oral administration is by 30~50 parts of walnut inner casing and 30~50 parts of glossy privet leaf system in parts by weight
At.
Present embodiment treatment canker sore is to be used together the joint medicine for oral administration of the Fermented Soybean as externally applied drug, in walnut
After shell and glossy privet leaf decoction, it is used as tea-drinking, daily 1 dose, continuously takes 1~2 week.Alleviation canker sore can effectively be treated.
The content of present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
The purpose of invention also may be implemented in contract sample.
Beneficial effects of the present invention are verified by following embodiment:
Embodiment 1
A kind of method preparing Fermented Soybean using composite bacteria fermentation of the present embodiment, it is to follow the steps below
:
Weigh semen sojae atricolor 100g, sweet wormwood 10g, mulberry leaf 10g are spare；
Step 1: the water that 2 times of weight are added impregnates 14h after taking semen sojae atricolor to be cleaned with clear water, drain, avoid light place is sent out
Bud processing is for 24 hours；
Step 2: adding water to decoct jointly 2 times, each 1h into sweet wormwood and mulberry leaf, merging filtrate is concentrated into 110mL, relatively
Density is 1.10~1.12g/cm3, for use；
Step 3: in the soybean concentrate of step 2 being added to after step 1 germination treatment, and be added and account for soybean weight
The green alum of amount 0.02% carries out immersion 12h；Sterilize boiling 40min under conditions of 121 DEG C, 0.105Mpa；Taking-up is placed in room temperature
It is cooling, obtain fermentation substrate；
Step 4: aseptically, fermentation substrate after taking step 3 boiling is 100g:2mL according to mass volume ratio
Ratio inoculation bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus mixed liquor (bacterial concentration 107-
109CFU/mL), prior to 28 DEG C culture 7d, then at 42 DEG C of culture 3d, then 50 DEG C or less dryings are to get Fermented Soybean；Wherein, withered
The mass ratio of careless bacillus, umbrella branch pears head mould and lactobacillus bulgaricus is 4:2:1.
Bacillus subtilis reference culture and umbrella branch pears head trichoderma strain are respectively Bacillus subtilis
Subsp.subtilis (CICC 24713), Lichtheimia ornata and Lactobacillusbugaricus, separate
It is dominant strain from Fermented Soybean medicine materical crude slice and through enzyme activity screening.
Embodiment 2
A kind of method preparing Fermented Soybean using composite bacteria fermentation of the present embodiment, it is to follow the steps below
:
Weigh semen sojae atricolor 100g, sweet wormwood 7g, mulberry leaf 7g are spare；
Step 1: the water that 2 times of weight are added impregnates 14h after taking soya bean to be cleaned with clear water, drain, avoid light place is sent out
Bud processing is for 24 hours；
Step 2: adding water to decoct jointly 2 times, each 1h into sweet wormwood and mulberry leaf, merging filtrate is concentrated into 100mL, relatively
Density is 1.10~1.12g/cm3, for use；
Step 3: in the soybean concentrate of step 2 being added to after step 1 germination treatment, and be added and account for soybean weight
The green alum of amount 0.02% carries out immersion 12h；Sterilize boiling 40min under conditions of 121 DEG C, 0.105Mpa；Taking-up is placed in room temperature
It is cooling, obtain fermentation substrate；
Step 4: aseptically, fermentation substrate after taking step 3 boiling is 100g:2mL according to mass volume ratio
Ratio inoculation bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus mixed liquor (bacterial concentration 107-
109CFU/mL), prior to 25 DEG C culture 7d, then at 40 DEG C of culture 3d, then 45 DEG C or less dryings are to get Fermented Soybean；Wherein, withered
The mass ratio of careless bacillus, umbrella branch pears head mould and lactobacillus bulgaricus is 4:3:2.
Bacillus subtilis reference culture and umbrella branch pears head trichoderma strain are respectively Bacillus subtilis
Subsp.subtilis (CICC 24713), Lichtheimia ornata and Lactobacillusbugaricus, separate
It is dominant strain from Fermented Soybean medicine materical crude slice and through enzyme activity screening.
Embodiment 1, embodiment 2 and the commercially available Fermented Soybean plasmin activity of different sources and isoflavone genin detection are tied
Fruit and control bacterial examination artificial situation such as table 1.
Table 1
From table 1 as it can be seen that commercially available Fermented Soybean plasmin activity is low, isoflavone genin content is lower, illustrates that soybean is different
The conversion ratio of flavonoid glycoside is low, and controls bacterium containing detection of Salmonella, escherichia coli, bile tolerance Gram-negative bacteria etc., and implements
Example 1, embodiment 2 plasmin activity be 4-9 times of commercially available Fermented Soybean, and Aglycones content is 4-40 times of commercially available Fermented Soybean, is said
Its bright activity and bioavilability are significantly increased, and without control bacterium, and illustrating it, there is no the risks containing pathogenic bacteria, use
Get up more safely, effectively.
The Fermented Soybean that Examples 1 and 2 are obtained carries out canker sore clinical treatment respectively；
Choose and suffer from canker sore case 120, the age at 20~60 years old, average age 34 years old, symptom: the oral cavity of patient
There are multiple ulcer to differ in size in mucous membrane, and surface covers canescence or yellow pseudomembrane, concavity, surrounding mucosa are red and micro-
Swollen, obvious cusalgia at ulcer seriously affects appetite, and some patientss are with halitosis, the symptom for dry and astringent headache of defecating, Duo Shuohui
Patient is recurrent exerbation.
Treatment standard:
Cure: cavity interior ulcer surface, which disappears, to heal, and no red and swollen, cusalgia is other to disappear with symptom.
Improve: ulcer surface number significantly reduces, and ulcer diameter is obviously reduced, other to disappear with symptom without red and swollen cusalgia.
Invalid: ulcer surface number, ulcer surface diameter, red and swollen cusalgia sense are without improvement, and there are still other with symptom.
120 patients are divided into 8 groups, respectively organize 1, group 2, group 3, group 4, group 5, group 6, group 7 and group 8；Every group using this
Invention different pharmaceutical treat specific as follows:
Medicine group 1: it is clayed into power using the Fermented Soybean of embodiment 1；Application method: before sleeping, Fermented soybean is spread on into trouble
Place, next day removal, continuous administration 3~7 days.
Medicine group 2: it is clayed into power using the Fermented Soybean of embodiment 2；Application method: before sleeping, Fermented soybean is spread on into trouble
Place, next day removal, continuous administration 3~7 days.
Medicine group 3: it is clayed into power using the Fermented Soybean of embodiment 1 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 1, the medicine for oral administration is by weight by 12 parts of cape jasmine, 30 parts of company
It sticks up, 30 parts of the root bark of white mulberry, 15 parts of Radix Codonopsis, 15 parts of Radix Astragali, 30 parts of Divine Comedy, 30 parts of hawthorn, 50 parts of malt, 15 parts
Fructus Aurantii, 15 parts of rhizoma atractylodis, 12 parts in one's early teens, the Radix Glycyrrhizae of 30 parts of Fermented Soybean, 30 parts of caragana and 10 parts composition；It will be above-mentioned
Drug is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Medicine group 4: it is clayed into power using the Fermented Soybean of embodiment 2 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 2, the medicine for oral administration is by weight by 12 parts of cape jasmine, 30 parts of company
It sticks up, 30 parts of the root bark of white mulberry, 15 parts of Radix Codonopsis, 15 parts of Radix Astragali, 30 parts of Divine Comedy, 30 parts of hawthorn, 50 parts of malt, 15 parts
Fructus Aurantii, 15 parts of rhizoma atractylodis, 12 parts in one's early teens, the Radix Glycyrrhizae of 30 parts of Fermented Soybean, 30 parts of caragana and 10 parts composition；It will be above-mentioned
Drug is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Medicine group 5: it is clayed into power using the Fermented Soybean of embodiment 1 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 1, the medicine for oral administration is by weight by 15 parts of Radix Codonopsis, 15 parts of Fu
Siberian cocklebur, 15 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of fennel seeds, 15 parts of Chinese yam, 15 parts of hawthorn, 15 parts of Divine Comedy, 15 parts of Fermented Soybean, 12 parts
Cortex Phellodendri and 6 parts Radix Glycyrrhizae composition；Said medicine is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Medicine group 6: it is clayed into power using the Fermented Soybean of embodiment 2 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 2, the medicine for oral administration is by weight by 15 parts of Radix Codonopsis, 15 parts of Fu
Siberian cocklebur, 15 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of fennel seeds, 15 parts of Chinese yam, 15 parts of hawthorn, 15 parts of Divine Comedy, 15 parts of Fermented Soybean, 12 parts
Cortex Phellodendri and 6 parts Radix Glycyrrhizae composition；Said medicine is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Medicine group 7: it is clayed into power using the Fermented Soybean of embodiment 1 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 1, the medicine for oral administration is by weight by 50 parts of walnut inner casing and 50 parts
Glossy privet leaf composition；Said medicine is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Medicine group 8: it is clayed into power using the Fermented Soybean of embodiment 2 as externally applied drug；
Externally applied drug application method: before sleeping, spreading on affected part for Fermented soybean, and next day removes, and continuous administration 3~7 days.
And medicine for oral administration is prepared with the Fermented Soybean of embodiment 2, the medicine for oral administration is by weight by 50 parts of walnut inner casing and 50 parts
Glossy privet leaf composition；Said medicine is fried into soup, takes and (usage: once a day, takes 1~2 week)；
Said medicine group 1-8 is respectively used to a group 1-8, dental ulcer treatment is carried out, the results are shown in Table 2.
Table 2
Typical case is as follows:
Case one
Zhang, female, 27 years old, canker sore recurrent exerbation 5 years or more, breaking-out was more difficult to fully recover compared with last time every time, lingual surface and two
The ulcer that cheek has multiple beans big, edge color is red micro- swollen, in great pain, the pharynx of diet difficulty, bitter to the taste, dry, big loose stool.Take this implementation
Drug 7 days of example medicine group 3, the state of an illness disappears, and canker sore is not reaccessed.
Case two
Liu, male, 35 years old, canker sore recurrent exerbation 3 years, breaking-out was more difficult to fully recover compared with last time every time, mucous membrane of mouth angle
The position for changing difference bears that ulcer is rounded or oval, and the ulcer of size, number not etc. around has blush, feels pain, send out repeatedly
Make, bitter to the taste, dry, big loose stool.Drug 7 days for applying the present embodiment medicine group 1, the state of an illness disappear, and canker sore is not reaccessed.
Case three
Zhang, male, 43 years old, canker sore recurrent exerbation 10 years, ulcer was small, and quantity is more, distributed more widely general, around ulcer
Mucous membrane is red and micro- swollen, and ulcer part cusalgia is obvious, big loose stool.10 days for applying the present embodiment medicine group 6, the state of an illness disappear, oral cavity
Ulcer is not reaccessed.
Case four
Slowly so-and-so, female 23 years old, canker sore 1 year, existing many places ulcer on the inside of oral cavity, surface covered yellow pseudomembrane, and surrounding is glutinous
Film is red and micro- swollen, and cusalgia sense is strong, and ulcer part cusalgia is obvious, big loose stool.7 days for applying the present embodiment medicine group 8, the state of an illness disappears
It loses, canker sore is not reaccessed.
It is understood that the principle that embodiment of above is intended to be merely illustrative of the present and the exemplary implementation that uses
Mode, however the present invention is not limited thereto.For those skilled in the art, essence of the invention is not being departed from
In the case where mind and essence, various changes and modifications can be made therein, these variations and modifications are also considered as protection scope of the present invention.
Claims (10)
1. a kind of method for preparing Fermented Soybean using composite bacteria fermentation, it is characterised in that it is followed the steps below:
Step 1: the water that 2~3 times of weight are added impregnates 12~16h after taking soybean to be cleaned with clear water, drain, avoid light place, into
24~48h of row germination treatment；
Step 2: adding water to decoct jointly 2 times, each 1h into sweet wormwood and mulberry leaf, merging filtrate, concentration is for use；
Step 3: in the soybean concentrate of step 2 being added to after step 1 germination treatment, and be added and account for soybean weight
0.02% green alum carries out 10~14h of immersion；Sterilize 30~40min of boiling under conditions of 121 DEG C, 0.105Mpa；Taking-up is set
It is cooling in room temperature, obtain fermentation substrate；
Step 4: aseptically, fermentation substrate after taking step 3 boiling is 100g:1~2mL's according to mass volume ratio
The mixed liquor of ratio inoculation bacillus subtilis, umbrella branch pears head mould and lactobacillus bulgaricus, prior to 25~28 DEG C culture 7d,
Then at 40~45 DEG C of culture 3d, then 50 DEG C or less dryings are to get Fermented Soybean；
Wherein, the mass ratio 100g:7-10g:7-10g of soybean, sweet wormwood and mulberry leaf.
2. a kind of method for preparing Fermented Soybean using composite bacteria fermentation according to claim 1, it is characterised in that soybean
For semen sojae atricolor or soya bean.
3. a kind of method for preparing Fermented Soybean using composite bacteria fermentation according to claim 1, it is characterised in that step
Amount of water is 18 times of weight of sweet wormwood and mulberry leaf in two.
4. a kind of method for preparing Fermented Soybean using composite bacteria fermentation according to claim 1, it is characterised in that described
Bacillus subtilis, the mould lactobacillus bulgaricus strain of umbrella branch pears head be isolated from Fermented Soybean medicine materical crude slice；Wherein, bacillus subtilis
For (Bacillus subtilis subsp.subtilis), it is preserved in Chinese industrial Microbiological Culture Collection administrative center, is protected
Hiding number are as follows: CICC 24713.
5. a kind of method for preparing Fermented Soybean using composite bacteria fermentation according to claim 1, it is characterised in that described
Bacillus subtilis and umbrella branch pears head mould mixed liquor concentration be 107~109CFU/mL。
6. a kind of method for preparing Fermented Soybean using composite bacteria fermentation according to claim 1 or 5, it is characterised in that withered
The mass ratio of careless bacillus, umbrella branch pears head mould and lactobacillus bulgaricus is 3~4:2~3:1.
7. the Fermented Soybean application as prepared by claim 1, it is characterised in that it is used to prepare the drug for the treatment of canker sore；It should
Drug is made of by Fermented Soybean grind into powder.
8. the Fermented Soybean application as prepared by claim 1, it is characterised in that it is used to prepare the drug for the treatment of canker sore；Institute
The drug for the treatment canker sore stated is by 10~12 parts of cape jasmine, 25~35 parts of Fructus Forsythiae, 25~35 parts in parts by weight
The root bark of white mulberry, 10~15 parts of Radix Codonopsis, 10~15 parts of Radix Astragali, 25~35 parts of Divine Comedy, 25~35 parts of hawthorn, 45~55 parts
Malt, 10~15 parts of Fructus Aurantii, 10~15 parts of rhizoma atractylodis, 10~12 parts in one's early teens, 25~35 parts of Fermented Soybean, 25~35 parts
Caragana and 8~10 parts Radix Glycyrrhizae composition.
9. the Fermented Soybean application as prepared by claim 1, it is characterised in that it is used to prepare the drug for the treatment of canker sore；Institute
The drug for the treatment canker sore stated is divided into medicine for oral administration and externally applied drug；The externally applied drug is Fermented soybean；Medicine for oral administration is by weight
Measuring number is by 10~15 parts of Radix Codonopsis, 10~15 parts of Poria cocos, 10~15 parts of Rhizoma Atractylodis Macrocephalae, 8~10 parts of fennel seeds, 10~15
Chinese yam, 10~15 parts of the hawthorn, 10~15 parts of Divine Comedy, 10~15 parts of Fermented Soybean, 10~12 parts of Cortex Phellodendri and 4~6 of part
The Radix Glycyrrhizae composition of part.
10. the Fermented Soybean application as prepared by claim 1, it is characterised in that it is used to prepare the drug for the treatment of canker sore；
The drug of the treatment canker sore is divided into medicine for oral administration and externally applied drug；The externally applied drug is Fermented soybean；Medicine for oral administration is pressed
Parts by weight are made of 30~50 parts of walnut inner casing and 30~50 parts of glossy privet leaf.
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Application publication date: 20190426 |