CN105727253A - Composition for protecting liver, clearing away toxic materials, preventing and treating liver and related metabolic diseases and preparation method thereof - Google Patents

Abstract

The invention relates to a composition for protecting liver, clearing toxin, preventing and treating liver and related metabolic diseases, which comprises the main materials of 200; the auxiliary materials comprise 5 parts of edible corn starch and 4 parts of sodium carboxymethyl cellulose, 6 parts of the auxiliary materials. The invention also provides a preparation method of the composition. The formula of the invention adopts natural traditional Chinese medicine materials with food and medicine homology, is supplemented with nutrient substances, has scientific compatibility, promotes medicine excretion, protects the liver, improves the function of liver cells, carries out non-medicine intervention on the problems of fatty liver and related metabolic systems, avoids secondary damage to the liver caused by medicine treatment by food and medicine homology, and achieves the effect of 'clearing liver and protecting liver without damaging the liver'.

Description

Protect the liver detoxicating prophylactic treatment liver and the compositions of correlated metabolism diseases and preparation method

Technical field

The present invention relates to field of health care products, a kind of protect the liver detoxicating prophylactic treatment liver and the group of correlated metabolism diseases Compound and preparation method thereof.

Background technology

Chinese medicine is mainly reflected in the following aspects to the understanding of liver physiological function.

1, main catharsis and harmonizing the functional activities of vital QI: under normal circumstances, liver is dirty with other to ensure itself according to its property reaching catharsis exactly The normal activity of internal organs.Catharsis function embodiment exists: one is to adjust spirit to go out to consider.The traditional Chinese medical science thinks that the spiritual feelings will of people has close with liver The relation cut, " Plain Questions LINGLAN secret allusion quotation opinion " is said: " liver person's organ of general, consider and here "." declaring five gas opinions " is also said: " liver Hide soul ", " considering " and " soul " is all the reflection of spirit feelings will, " Ling Shu Miraculous Pivot or Divine Axis itself " piece cloud: " deficiency of liver-QI is then feared, actually anger ", Tang Rongchuan says: " night, then soul was attributed to liver and for sleeping." these discussions all reflect the spiritual feelings will of people and the relation of liver, liver catharsis Normally, mechanism of qi is adjusted and is reached, then spirit is smooth happy, and the change in terms of its normal manner then causes mind, in turn, any feelings will are lost in catharsis Stimulate and the most all may result in the pent-up of irritability and against random, the two reciprocal causation.Simultaneously as depression and stagnation of QI, blood often can be caused to be subject to Resistance, qi depression to blood stasis, or be that QI and blood is inverse disorderly." Plain Questions vital QI of the body connecting with natural QI opinion " is said: " excessive anger leading to segregation of QI from the configuration and blood stagnating in the upper part of the body make people Emotional syncope." clinical findings depression of liver-QI person has two side of body distending pains, uncomfortable in chest smooth more, women then has the phenomenons such as menoxenia, all illustrates The catharsis effect of liver and the relation of QI-blood circulation.It two is facilitating digestion, to help absorption, through cloud: " food gas enters stomach, the spleen transporting essential substances to the liver ", What digestive and absorptive functions was movable is normally carried out needing the secretion of the lifting of QI of the spleen and stomach and bile, and taste lift and the secretion of bile Have to rely on again the normal catharsis of liver, if catharsis imbalance arises that corresponding gastrointestinal symptom, as vexed in inappetence, gastral cavity Abdominal distention or diarrhea with loose stool etc..

2, main store blood and regulate blood fortune: " six joint hide as opinion " say: " liver person ... its China at pawl ... with hemopoietic gas, " " plain Ask the five internal organs generation " say: " old friend sleeping and blood is attributed to liver ", Lee stile " Elementary Medicine " also mention " the dynamic then blood of people runs all warps, People is quiet, and blood is attributed to liver ", these are all regulation effects liver being described for blood volume.Blood keeps certain at human body and weighs, For the needs of body, when human body carries out aggravating activities or is engaged in other activities daytime, body needs blood volume the most, and works as night When sleep or rest, then body needs blood volume to reduce accordingly.When needing blood volume many, liver just discharges the excess blood of storage；Need blood volume Time few, the most unnecessary blood volume returns again liver.Ancients think that liver is the viscus of storing blood, this physiological process of the store blood homergy to human body It is very important, so Tang Rongchuan says: " always controlling all mass formed by blood stasis nothing more than reason liver also ", adds: " person of enriching blood with tonifying liver is always Want ".

3, nourishing muscle and tendon arteries and veins and fill profit onyx: through cloud: " food gas enters stomach, and the spleen transporting essential substances to the liver, excess of QI is in muscle ", " Plain Questions innocence in ancient times opinion " Also say: " irritability declines, the tendon being unable to move ".Visible, muscle arteries and veins be rely on liver moisten support could maintain its normal function.Lee stile says: " person all biceps of moving are done, liver nourishing muscle and tendon." liver blood abundance, muscle arteries and veins obtains it and moistens, the most strong and powerful, bends and stretches flexibly, otherwise just There will be biceps feeble and exhausted, extremity are aching and limp unable, and the tired body of body is lazy, is frequently seen these symptoms in hepatopathy.

4, liver is upper by blood supports two mesh: the traditional Chinese medical science thinks that liver is in close relations with mesh, through cloud: " normal vision relying on sufficiency of liver-blood ", " Ling Shu Miraculous Pivot or Divine Axis arteries and veins Degree opinion " also say: " liver-QI being connected with eyes, liver and then mesh can debate the five colors ".Deficiency of liver-blood then poor vision, dim-sighted, bitterly clinically Swollen, dry and astringent, " gas becomes big opinion " is sayed that " edema with the liver involved is by strongly fragrant ... conjunctival congestion with ophthalmalgia all infectionss " then refers to the oculopathy caused by flaming up of liver-fire, it is seen that liver Being the closest with the relation of two mesh, so ancients' meaning " liver opening at eye ", the traditional Chinese medical science is recognized very early and is controlled with animal livers Treating blindness is also good example.

In a word, liver just because of possessing above-mentioned physiological function, the property of its fluent nature of guarantee, lift mechanism of qi, have enough to meet the need QI and blood, Facilitating digestion absorbs, and maintains the nutrition of muscle arteries and veins muscle, and these theories have important directive significance for the medication of hepatopathy.

The Overall View of the traditional Chinese medical science is thought, viscera QI and blood is an organic whole, ensures its physiology by mutually coordinated and restriction Completing of function, liver is as the same.For liver, its normal physiological function depends on the nourishing of kidney water, making moist of nutrient blood, lung The restriction of gold, the cultivation of spleen soil, then it reaches property of smooth cyclopentadienyl in side, must be soft body, for anosis liver, if four mistake one, Holding concurrently or disorder of QI and blood, the most variable raw disease, the catharsis week rotating function of liver contributes to again the lifting of taste mechanism of qi, the disappearing of diet simultaneously Change and absorb, lung qi and a surname sends out and compress, the excretion of bile and the turnover of QI and blood, they are the organic wholes of a vital movement, Common coordination, maintains the balance of internal organs QI and blood.

For modern medical theory, liver has following functions.

One, metabolic function: 1. carbohydrate metabolism: becoming glucose after starch in diet and carbohydrate digestion through intestinal absorption, liver will Its synthesis liver glycogen storage gets up；When body requirement, hepatocyte can be that glucose utilizes for body hepatic glycogenolytic again.② Protein metabolism: liver is the unique synthetic organ of human albumin；Globulin, pheron and plasma protein beyond γ ball egg Generation, maintain and regulate will liver participate in；The process of amino acid metabolism such as desamination reaction, urea synthesis and ammonia is all liver Carry out in dirty.3. lipid metabolism: the synthesis of fat and release, fatty acid decomposition, ketoplasia and oxidation, C/PL Synthesis, lipoprotein synthesis and transport etc. all carry out in liver.4. vitamin metabolism: many vitamin such as A B C D and K Synthesis with store all closely related with liver.Liver obvious impaired time to there will be vitamin metabolism abnormal.5. hormone metabolism: liver The inactivation of dirty participation hormone, may occur in which gonadal hormone disturbance when liver function long-term damage.

Two, bile generates and excretion: bilirubinic picked-up, combines and drains, and generation and the excretion of bile acid are all undertaken by liver. Hepatocyte manufactures, the bile of secretion, is transported to gallbladder through bile duct, and gallbladder drains into small intestinal after concentrating, help fat digestion and Absorb.

Three, Detoxication: more produced harmful wastes and external poisonous substance, toxin, the generation of medicine during body metabolism Thank and catabolite, all at liver detoxification.

Four, immunologic function: liver is maximum reticuloendothelial cell phagocytosis system, and it can be by swallowing, isolating and eliminate invasion Various antigens with interior life.

Five, coagulation function: almost all of thrombin is all manufactured by liver, liver is in human body blood coagulation and two systems of anticoagulant Dynamic equilibrium plays important regulation effect.The order of severity that liver function is destroyed often is paralleled with the degree of blood coagulation disorders, clinically Some liver cirrhosis patient common causes hemorrhage even dead because of hepatic failure.

Six, other: liver participate in the regulation of human body blood volume, the generation of heat and water, the regulation of electrolyte.Such as liver injury Time to sodium, potassium, ferrum, phosphorus, etc. electrolyte Imbalance, it can be common that the retention in vivo of water sodium, cause edema, ascites etc..

But, along with the pollution of environment, bad living habit and the damage of chemicals, the sickness rate of hepatopathy is more and more higher.

Hepatopathy includes acute hepatitis, chronic hepatitis, fatty liver disease, alcoholic liver disease, autoimmune liver disease, liver cirrhosis and constitutional The multiple disease such as hepatocarcinoma, is that threat human health is one of principal disease now.China 1-59 year population hepatitis B surface antigen Carrying rate is 7.18%, and c-hepatitis antibody positive rate is 3.2%, existing Chronic Patients with HBV Infection about 93,000,000 people, the most slowly Property hepatitis B patient about 20,000,000 example, recently as growth in the living standard, fatty liver, the patient of alcoholic liver disease get over Come the most.

China is the big country that hepatopathy is occurred frequently, and the medical expense paid because of various hepatic disease every year is up to 50,000,000,000 yuans. The fatty liver that chemical liver injury and a variety of causes cause becomes the common disease that China is the most occurred frequently.Epidemiological study shows Chinese's pathogenesis of fatty liver rate is about 22-25%, and its sickness rate is gradually increasing along with the raising of people's living standard.Such as clinical Epidemiological study shows: the pathogenesis of fatty liver rate that Shandong Province's population chemical liver injury and a variety of causes cause is about 33-35%, The pathogenesis of fatty liver rate that Beijing's population chemical liver injury and a variety of causes cause is about 25%, and Shanghai Population chemical liver damages Injure the pathogenesis of fatty liver rate that a variety of causes causes and be about 20%, the fat that Shenzhen's population chemical liver injury and a variety of causes cause Fat liver sickness rate is about 17%.If hepatocellular degeneration moderate or severe fatty liver are treated not in time, in 10 years, develop into liver fiber Changing and the ratio of liver cirrhosis is up to 70%, the patient with liver cirrhosis of about 20-30% develops into hepatocarcinoma patient the most at last.

Being three points of poison of medicine, liver itself can be caused secondary injury by common pharmacotherapy.

Patent of invention " a kind of compound recipe for liver protecting and preparation method and application " (Publication No. CN 101480442A) is public Open one to be made up of Herba Taraxaci, Pollen Pini, Flos Lonicerae, Poria, Fructus Gardeniae, Radix Puerariae, vitamin C, zinc gluconate, There is the liver protecting, recover liver function, improve the effect of liver detoxification.But Herba Taraxaci heavy dose of in this prescription and Fructus Gardeniae taste Bitterness is trembled with fear, and injures one's stomach, and insufficiency of the spleen loose stool person should not use, the most cold and cool for whole prescription.But, the relation of mutual promotion between the five internal organs, The raw heart of liver is exactly wood generating fire, if blood stored in the liver is with the heart that helps；The raw spleen of the heart is exactly fire generating earth, and the yang-energy such as the heart can ask spleen；The raw lung of spleen is just Being earth generating metal, the vital essence such as spleen transporting and transforming nutrients from foodstuff can be with lung benefiting；The raw kidney of lung is exactly metal generating water, then functional activities of the body fluid as respectful in normal lung-QI descending as a means of Kidney；The raw liver of kidney is exactly water generating wood, if kidney storing essence is to nourish cloudy blood of liver etc..Restriction or checking relation in five elements relation between the five internal organs: lung (golden) Clear respectful decline, can suppress the rising of liver (wooden) sun, i.e. metal restricting wood；The bar of liver (wooden) reaches, and can dredge the heap soil or fertilizer over and around the roots of purgation of spleen excess (native) Stagnant, i.e. Wood restricts earth；The transporting of spleen (native), is possible to prevent spreading unchecked of kidney (water) water, i.e. earth restricting water；Cloudy upper of kidney (water) Ji, can restrict the heart (fiery) yang hyperactivity strong, i.e. water restricting fire；The heart (fiery) sun heat, can restrict lung (golden) the most respectful too, I.e. fire restricting metal.Therefore, this prescription universality is strong, and has damaged taste after taking.

Summary of the invention

According to above-mentioned weak point, it is an object of the invention to provide a kind of gentleness, adaptable protect the liver detoxicating prophylactic treatment Compositions of liver and correlated metabolism diseases and preparation method thereof.

For achieving the above object, technical program of the present invention lies in: one protects the liver detoxicating prophylactic treatment liver and correlated metabolism disease Sick compositions, according to the mass fraction, its major ingredient be Radix Puerariae 200-250 part, Poria 200-220 part, Herba Taraxaci 50-60 part, Radix Glycyrrhizae 40-50 part, Herba Cichorii 160-180 part, soybean oligo saccharide 5-6 part, soy peptide powder 10-15 part, glycine 5-8 part, kind Lycopene 2-3 part；Its adjuvant is edible corn starch 5-6 part, sodium carboxymethyl cellulose 4-6 part.

Preferably: described Radix Puerariae, Herba Taraxaci are handled as follows:

(1) Radix Puerariae, Herba Taraxaci are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) weigh the Radix Puerariae coarse powder of 200-250 mass fraction and the Herba Taraxaci coarse powder of 50-60 mass fraction, with solid-liquid ratio be It is the ethanol water of 40-60% that the ratio of 1:5-10 adds volumetric concentration, ultrasonic immersing 6-8h at a temperature of 40-60 DEG C, filters；

(3) filtering residue is taken, the ratio addition volumetric concentration ethanol water as 40-60% with solid-liquid ratio as 1:1-2,40-60 DEG C At a temperature of ultrasonic immersing 6-8h, filter, twice filtrate merged, is evaporated to without ethanol；

(4) solution is dialysed, take filtrate and be spray-dried, obtain Radix Puerariae, Herba Taraxaci extract.

Preferably: described Poria, Radix Glycyrrhizae are handled as follows:

(1) Poria, Radix Glycyrrhizae are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) the Poria coarse powder of 200-220 mass parts and the Radix Glycyrrhizae coarse powder of 40-50 mass fraction are weighed, with solid-liquid ratio as 1:5-8 Ratio add pure water, at 45-65 DEG C heated at constant temperature soak 20-30min, heated and boiled 20-30min, filter；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:1-2 adds pure water, and at 45-65 DEG C, heated at constant temperature soaks 20-30min, Heated and boiled 20-30min, filters, and continues to be heated to boiling, and be stirred continuously after twice filtrate being merged, and makes Poria, sweet Grass fluid extract is standby.

Preferably: described Herba Cichorii is handled as follows:

(1) Herba Cichorii is cleaned, pulverize after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) joining in container by Herba Cichorii coarse powder, the ratio with solid-liquid ratio as 1:0.8-1 adds 70% ethanol solution, and stirring is all Even, moistening airtight standing 1-2h；

(3) the Herba Cichorii coarse powder after infiltration is transferred in percolator, suitably presses, after dipping 24-36h, with 1-3ml/min's Rate collection extracting solution；

(4) by extracting solution concentrating under reduced pressure, until without ethanol；

(5) concentrate, be dried, obtain Herba Cichorii extract.

The present invention also provides for protecting the liver the preparation technology of the compositions of detoxicating prophylactic treatment liver and correlated metabolism diseases, including as follows Step:

(1) weigh: weigh soybean oligo saccharide 5-6 part, soy peptide powder 10-15 part, glycine 5-8 part, lycopene 2-3 Part, edible corn starch 5-6 part, sodium carboxymethyl cellulose 4-6 part, individually deposit；

(2) pelletize: Radix Puerariae and Herba Taraxaci extract, Poria cocos and liquorice fluid extract, Herba Cichorii extract move into efficient wet granulator After middle mix homogeneously, add soy peptide powder, glycine, lycopene, sodium carboxymethyl cellulose, again mix homogeneously, make Granule；

(3) cladding: add soybean oligo saccharide, edible corn starch after step (2) makes granule, by soybean oligo saccharide It is evenly coated at granule appearance with edible corn starch；

(4) it is dried: wet granular is moved into Fast granulate machine dispersion and granulation, then moves in drying and granulating machine dry, until granule dries Dry；

(5) granulate: dry granule is used Fast granulate machine granulate, is dispersed into uniform granule；

(6) tabletting: granule uses sheeting equipment carry out tabletting；

(7) packaging: using automatic packaging machine packaging, case after luggage box of going forward side by side again, warehouse-in preserves.

Radix Puerariae sweet in the mouth in above-mentioned prescription, pungent, cool in nature, return spleen, stomach, lung, bladder warp, have relieving muscles diaphoresis, invigorating YANG QI to promote eruption, solve Effect that heat is promoted the production of body fluid.For treating diarrhea due to hypofunction of the spleen, calentura is thirsty, cure mainly fever caused by exogenous pathogens, and pain of the head and nape is strong, measles without adequate eruption, The diseases such as epidemic febrile disease is thirsty, quenches one's thirst, alcoholism, obstruction of qi in the chest and cardialgia.Often food Amylum Puerariae Radicis can regulate function of human body, health invigorating, improves body Resistance against diseases, anti-ageing prolongs life, vigor of remaining youthful forever.Modern medicine study, Radix Puerariae flavone has cancer-resisting and estrogen-like is made With, women skin care can be promoted, especially obvious to middle-aged women and menopausal women face-nourishing health-care effect.The main component of Radix Puerariae is Starch, contains the flavone compound of about 12% in addition, and including Semen sojae atricolor (Semen Glycines) glycoside, daidzein, puerarin etc., more than 10 plant； And containing daucosterol, aminoacid, Coumarins etc.；Also have protein, aminoacid, sugar and the ferrum of needed by human, calcium, The mineral such as copper, selenium, is all-ages famous and precious tonic, has the good reputation of " Radix Ginseng in thousand "." wound as far back as Han dynasty Zhang Zhongjing Cold opinion " in just have " GEGEN TANG " this famous prescription, be still the important side of inducing sweat so far." book on Chinese herbal medicine justice " meaning Radix Puerariae " The gas of taste sun clearly can be developed ".

Poria sweet in the mouth, light, property is put down, GUIXIN, lung, spleen, kidney channel, for edema oliguria, and phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, just Diarrhea with loose stool is rushed down, irritability, palpitation with fear insomnia.Ancients' Poria is called " god's medicine when four ", because its effect is widely, regardless of four Season, by it with various compatibility of drugss, the coldest, temperature, wind, wet all diseases, effect of its uniqueness can be played.Modern medicine grinds Studying carefully, Poria energy enhancing human body immunity function, pachyman has notable antitumor and protects the liver dirty effect.

Soybean oligo saccharide is the general name of solubility saccharic in Semen sojae atricolor, and main component refers to sucrose (disaccharidase), the cotton that monosaccharide number is 3-4 Son sugar (trisaccharide) and stachyose (tetrose) etc..The effect of soybean oligo saccharide has: 1, the clean intestinal of relieving constipation: constipation patient be mostly because of Enteral lacks caused by bacillus bifidus.Especially old people.With age.Enteral bacillus bifidus gradually decreases and easily suffers from Constipation.Test proves, Healthy People absorbs 3 grams of soybean oligo saccharide every day, just can promote bifidobacterium growth, produces defecating feces excretion. Soybean oligo saccharide can also promote that excretion is accelerated in enterokinesia.2, bacillus bifidus propagation in intestinal is promoted: prove through experimentation, every day Taking in 10-15 gram of soybean oligo saccharide, after 17 days, bacillus bifidus can be increased to 45% by original 0.99%.Double in intestinal Discrimination bacillus is particularly easy to utilize soybean oligo saccharide, produces acetic acid and lactic acid and some phage speciess, thus suppresses exogenous and cause a disease Bacterium and enteral are because there being the propagation of Putrefying bacteria: bacillus bifidus, also by phosphatidic acid and intestinal mucosal surface, forms one layer and has protection The biology of effect, envelope barrier, thus prevent harmful microbe invasion and surely grow.3, serum cholesterol is reduced: bacillus bifidus Directly affect and disturb the activity of p-footpath base-p-methyl glutaryl enzyme A reductase, it is suppressed that the synthesis of cholesterol, make serum gallbladder Sterin reduces.4 and the liver protecting: long-term soybean oligo saccharide of taking in can reduce the generation of internal toxic metabolite material, alleviates liver The burden of removing toxic substances, so also have certain effect in terms of preventing and treating hepatitis and prevention liver cirrhosis.5, the life of enteral corrupt substance is suppressed Become: prevent and treat constipation and diarrhea.

Herba Taraxaci bitter but sweet flavor, property is flat to be slightly cold, and enters liver, stomach warp, has heat-clearing and toxic substances removing, effect of diuresis eliminating stagnation, cure mainly acute breast Adenitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, Gastritis, hepatitis, cholecystitis, urinary tract infection.Modern study shows, Herba Taraxaci herb containing taraxasterol, choline, inulin, Pectin etc., have antibacterial, the effect of antitumor, function of gallbladder promoting.

Radix Glycyrrhizae is the root and rhizome of herbaceos perennial Radix Glycyrrhizae, and nature and flavor are sweet flat, GUIXIN, lung, spleen, stomach warp.Tool invigorating the spleen and replenishing QI, Nourishing the lung to arrest cough, relieving spasm to stop pain, the merit of the mitigation property of medicine, its effective ingredient is glycyrrhizin, enoxolone, 4',7-Dihydroxyflavanone, Radix Glycyrrhizae Polysaccharide is one of Chinese herbal medicine of commonly using clinically.Radix Glycyrrhizae also has antiinflammatory, anti-allergic effects, and the throat of inflammation and trachea can be protected to glue Film.Radix Glycyrrhizae extractum and glycyrrhizic acid have the Detoxication of similar glucuronic acid to some poisonous substance.Subacid contained by Radix Glycyrrhizae can block cause The effect of cancer thing induced tumor growth.

Herba Cichorii mildly bitter flavor, salty, cool in nature, return spleen, liver, wing, Guang warp, there is liver heat removing function of gallbladder promoting, invigorating the stomach and promoting digestion, inducing diuresis to remove edema Effect, for jaundice due to damp-heat, lack of appetite of having a stomachache, edema oliguria.Chicoric acid is one of immune active ingredient particularly important in Herba Cichorii, Modern medicine study shows, chicoric acid has enhancing immunologic function and the effect of antiinflammatory, and can suppress hyaluronidase, protects glue Former albumen is from the impact of the free radical that may result in degraded.

Soy peptide powder be a kind of be made up of 2-6 aminoacid, molecular weight is at the hybrid peptide product of 200-800Dalton.Soybean peptide Powder has perfect nutritive value, quickly supplements the whole protein nutrition of needed by human body, improves immunity；Can quickly repair flesh Meat damage, promotion wound healing；Regain one's strength, strengthen muscle strength, improve motor capacity；Reduce pathologic exception leukocyte Increase, improve the mental status of old people, slow down aging；Regulation reduces blood fat, blood pressure, cholesterol；Antioxidation, beauty treatment； Improve sleep；Sport people post exercise muscular soreness can be alleviated, strengthen exercise tolerance, improve motor capacity；To α in human body- Glucosidase has slow inhibitory action, glucose in energy decomposer rapidly, when being used together with other carbohydrates and saccharide, Do not affected by amount of insulin secretion, the effect that suppression blood glucose rises rapidly can be played；Soybean protein through special biological enzyme action Many Toplink stimulate the secretion of internal thyroxin, promote the bile acidification of cholesterol, reduce the intestinal absorption to cholesterol, and It is promoted to excrete with feces；Soybean protein polypeptide through special biological enzyme action can suppress Angiotensin-Converting (ACE) Activity.ACE is a kind of carboxylic dipeptidase, and angiotensin I generates the liter blood pressure bioactive peptide that activity is the strongest under the effect of ACE Angiotensin, the latter's vasoactive smooth muscle, causes vasoconstriction, makes increasing of blood pressure persistence.Suppress as ACE The soybean peptide of agent, it is possible to reduce the formation of angiotensinⅡ, anti-hemostatic tube end-contraction, reach the purpose of blood pressure lowering.

Glycine is the most basic material constituting protein, and in the composition of human body cell, protein accounts for the 50% of dry cell weight. The constituent of the various enzymes of various biochemical reactions in protein or participant's health.Therefore, glycine is that human body composition must not The composition that can lack.The effect of glycine is as follows: 1, reduces the cholesterol concentration in blood, prevents and treats hypertension；2, blood is reduced In blood glucose value, prevent and treat diabetes；3, blood clotting, thrombosis can be prevented and treated；4, improve muscle vitality, prevent hyperchlorhydria.Separately have Research shows, glycine can be stoped and exhausted ATP by anti-histanoxia and effectively protect renal proximal tubules and hepatocyte, Glycine can also alleviate liver reperfusion injury by microcirculation in promoting liver.The major pathologic features of fatty liver is hepatocyte fat Degeneration and necrosis, wherein liver inner stellate cell (Kupffer cell) produces the cytokine of release, such as tumor necrosis factor (TNF- α) play a major role, vivo oxidation reducing condition disequilibrium, when producing excessive free radicals, (including ROS, NOS etc.), In blood, level of endotoxin (such as LPS) is significantly raised, activates Kupffer cell release TNF-α in liver.Recently research have indicated that, Glycine protection hepatocyte, the mechanism stoping Kupffer cell release TNF-α is that it is thin that glycine can activate open Kupffer In after birth glycine mediation chloride channel (Glycine-gated Chloride Channel), increase Chlorion influx (raise 2～ 3 times), make cell membrane polarity raise, stabilize Kupffer cell membrane, make Ca++ ion channel to open, thus blocked calcium (endotoxin mainly by stimulating Kupffer cell membrane, activates flow of calcium ions for the TNF-α synthesis that in ion, stream causes and secretion Release TNF-α).

Lycopene is primarily present in the mature fruit of plant of Solanaceae Fructus Lycopersici esculenti.It is to be found in the plant of nature at present One of powerful antioxidant.Science proves, singlet oxygen and oxygen-derived free radicals in human body are infringement human body self immune system Arch-criminal.Lycopene is removed effect of free radical and is outclassed other carotenoid and vitamin E, its singlet-oxygen quenching Speed constant is 100 times of vitamin E.It can be with effectively preventing because of aging, the various diseases that immunity degradation causes.Kind Lycopene, by connecting between inducing cell, strengthens the GJIC between normal cell, controls cell growth and Cell differentiation inducing activity presses down The growth of tumor processed.Lycopene is a kind of low cholesterol agent, and it can suppress macrophage 3-hydroxy-3-methylglutaric acid list acyl auxiliary Enzyme A, and it is the rate-limiting enzyme of a kind of Biosynthesis of cholesterol.Experiment finds, adds Fructus Lycopersici esculenti in the medium cultivating macrophage After red pigment, its cholesterol biosynthesis reduces, and lycopene also increases macrophage low density lipoprotein, LDL (LDL) receptor active simultaneously. Experiment it is also shown that human body in 3 months every day supplement 60mg lycopene, can reduce by the kytoplasm LDL-C concentration of 14%. The above-mentioned biological characteristics of lycopene determines it to be had antioxidation, mutation inhibiting, reduction nucleic acid damaging, reduces cardiovascular disease The plurality of health care functions such as disease and prophylaxis of cancer.

For theory of Chinese medical science, the patient of various acute and chronic hepatitis, often show as delay, deadlocked, obstinate, difficult more etc. Damp and hot disease feature, therefore, " hepatitis virus " is referred to as " epidemic disease caused by damp-heat pathogen poison " or " noxious dampness " by most scholars.From Chinese medical discrimination From the point of view of, viral hepatitis belongs to " epidemic febrile disease " category, and with damp and hot as cause, liver spleen is with sick.Pathogenesis change mainly toxic dampness and heat accumulates Knot taste, are stranded and hold back liver and gall, so that transporting and transforming function of the spleen and stomach loses strong, irritability catharsis is not normal.Its mechanism is: epidemic disease caused by damp-heat pathogen poison sustainable existence body In, damp and hot gradually being goed deep into blood system by edema caused by disorder of QI, no matter weight in wet base, thermogravimetric or damp and hot lay equal stress on, damp and hot long bridle all may result in blood stasis, by In dampneee being viscous and lingering, damp and hot the most easily block mechanism of qi, the stagnation of QI then blood stasis；Wet easy heat-transformation, the easy stasis of blood of heat in blood then blood are stagnant, and then are formed damp and hot Ecchymosis is demonstrate,proved.If damp and hot stifling, liver and gall are lost and are let out, the bile flowing outside the biliary passages and excessive skin, then occur that icteric sclera, skin be yellow, yellow urine. Clinical liver disease is observed it will be seen that in different types of liver disease state of an illness chronicity, worsening, activeness stage, patient often shows as hepatic region Scorching hot distending pain or stomachache, painful area fixes, dry mouth with bitter taste, is not intended to polydipsia, icteric sclera, color of the skin being yellow as peel of orange, and urine is turned to be yellow, Even such as strong tea sample, feeling of oppression and fullness in the chest and epigastrium or distending pain, have blood in stool, liver palm, red thread or petechia, ecchymosis, yellowish fur, tongue seen from skin The performances such as matter is dark red, the profound cunning of arteries and veins, hepatosplenomegaly, all can belong to hot and humid ecchymosis and become trouble mutually.The pathology that various acute, chronic hepatitis relate to Factor mainly includes wet, hot, strongly fragrant, the stasis of blood, poison etc., and its pathogenic process shows as retention of damp-heat in the interior → fire-transformation → wine poison → blood stasis → heresy The disease conversion characteristic that poison wound is positive.Therefore, the damp and hot initiating agent being hepatopathy and falling ill, hot and humid ecchymosis is tied in incidence of hepatitis mutually Thimble saves.Wet severe one, indigestion and loss of appetite, detest greasy, heaviness of the head as if it were tightly bandaged, limbs are heavy, abdominal distention loose stool, white and greasy fur, rolling pulse；Thermogravimetric Person, thirst and liking drink, oliguria with reddish urine, constipation, can have heating, yellowish fur, rapid pulse；Damp and hot and severe one, both symptoms Double see.

Li Dong Yuan is pointed out: " abundance of vigour, is all filled by QI of the spleen and stomach, then can nourish vigour ", highlights taste to vigour The effect of supplementing nutrition.Whether " congenital essence " or " acquired essence ", it is required for taste and nutrition, ability are provided continuously Contain and do not wane, being normally carried out of the activity that sustains life.Taste maintenance is the most important thing of health preserving as can be seen here.

In this prescription, Radix Puerariae and Herba Taraxaci are monarch drug, and the cause of hepatopathy is alleviated and eliminated to heat-clearing and toxic substances removing pathogenic fire reducing from root；It is aided with Herba Cichorii is assistant officer's medicine, further depressed liver-energy dispersing and function of gallbladder promoting, main catharsis, helps the damp and hot discharge in patient body；Poria is adjuvant drug, eliminating dampness and diuresis, Assist a ruler in governing a country assistant officer's medicine dehumidifying；Radix Glycyrrhizae, for making medicine, plays the effect relaxing each property of medicine, furthermore, Radix Glycyrrhizae invigorating the spleen and replenishing QI, relieving spasm to stop pain, slow Solve pain when patient's disease is sent out, supplement my vigour simultaneously, help patients ' recovery.Soybean oligo saccharide, glycine can reduce blood Clearing gallbladder sterin, can prevent and treat blood clotting, thrombosis.Soybean oligo saccharide can also reduce internal toxic metabolite material and produce, and alleviates liver detoxification Burden.Little molecule soy peptide powder easily absorbs, help patient supplement high quality protein, beneficially pathological changes hepatocyte repair with again Raw.Poria, lycopene can reduce interior free yl, enhancing human body immunity power.

The prescription of the present invention integrate heat clearing away, dampness removing, removing heat from blood, invigorate blood circulation, all methods such as removing toxic substances are treated, and are effectively improved patient Damp and hot disease.Meanwhile, by being in harmonious proportion patient's taste and liver and gall, metabolic function is improved, beneficially the recovery of patient's vigour.3rd, By supplementary high quality protein, patient is helped to repair the hepatocyte of pathological changes.4th, by improving patient's autoimmunity, strengthen Resistance against diseases and raising rehabilitation ability.

Prescription of the present invention, by using the Chinese medicine material of natural Medicine Food Homology, is aided with nutrient substance, the compatibility of science, promotes medicine Thing is drained, and the liver protecting improves stem cell function, the problem of fatty liver and associated metabolic system is carried out Non-medicine intervention, food Medicine homology avoids the secondary injury that liver is caused by Drug therapy, reaches the effect of " liver heat removing hepatoprotective not impairing the liver ".

Specific embodiment mode

Below in conjunction with specific embodiment, the present invention will be further described.

Embodiment 1

A kind of detoxicating prophylactic treatment liver and compositions of correlated metabolism diseases of protecting the liver, according to the mass fraction, its major ingredient is Radix Puerariae 200 parts, 200 parts of Poria, Herba Taraxaci 50 parts, 40 parts of Radix Glycyrrhizae, Herba Cichorii 160 parts, soybean oligo saccharide 5 parts, soy peptide powder 10 parts, glycine 5 parts, lycopene 2 parts；Its adjuvant is edible corn starch 5 parts, sodium carboxymethyl cellulose 4 parts.

Wherein, Radix Puerariae, Herba Taraxaci are handled as follows:

(1) Radix Puerariae, Herba Taraxaci are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20 mesh aperture sieve；

(2) weighing Radix Puerariae coarse powder and Herba Taraxaci coarse powder, the ratio with solid-liquid ratio as 1:5 adds the ethanol water that volumetric concentration is 40% Solution, ultrasonic immersing 6h at a temperature of 40 DEG C, filters；

(3) taking filtering residue, it is the ethanol water of 40% that the ratio with solid-liquid ratio as 1:1 adds volumetric concentration, at a temperature of 40 DEG C Ultrasonic immersing 6h, filters, and twice filtrate is merged, is evaporated to without ethanol；

(4) solution is dialysed, take filtrate and be spray-dried, obtain Radix Puerariae, Herba Taraxaci extract.

Poria, Radix Glycyrrhizae are handled as follows:

(1) Poria, Radix Glycyrrhizae are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20 mesh aperture sieve；

(2) weighing Poria coarse powder and Radix Glycyrrhizae coarse powder, the ratio with solid-liquid ratio as 1:5 adds pure water, heated at constant temperature leaching at 45 DEG C Bubble 20min, heated and boiled 20min, filters；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:1 adds pure water, and at 45 DEG C, heated at constant temperature soaks 20min, and heating is boiled Boiling 20min, filters, and continues to be heated to boiling, and is stirred continuously, make Poria, Radix Glycyrrhizae fluidextract after twice filtrate being merged Standby.

Herba Cichorii is handled as follows:

(1) Herba Cichorii is cleaned, pulverize after cleaning, coarse powder is crossed 20 mesh aperture sieve；

(2) joining in container by Herba Cichorii coarse powder, the ratio with solid-liquid ratio as 1:0.8 adds 70% ethanol solution, stirs, Moistening airtight standing 1h；

(3) the Herba Cichorii coarse powder after infiltration is transferred in percolator, suitably presses, after dipping 24h, with the speed of 1ml/min Collect extracting solution；

(4) by extracting solution concentrating under reduced pressure, until without ethanol；

(5) concentrate, be dried, obtain Herba Cichorii extract.

The preparation technology of the compositions protecting the liver detoxicating prophylactic treatment liver and correlated metabolism diseases of the present invention, comprises the steps:

(1) weigh: weigh soybean oligo saccharide 5 parts, soy peptide powder 10 parts, glycine 5 parts, lycopene 2 parts, eat Corn starch 5 parts, sodium carboxymethyl cellulose 4 parts, individually deposit；

(2) pelletize: Radix Puerariae and Herba Taraxaci extract, Poria cocos and liquorice fluid extract, Herba Cichorii extract move into efficient wet granulator After middle mix homogeneously, add soy peptide powder, glycine, lycopene, sodium carboxymethyl cellulose, again mix homogeneously, make Granule；

(3) cladding: add soybean oligo saccharide, edible corn starch after step (2) makes granule, by soybean oligo saccharide It is evenly coated at granule appearance with edible corn starch；

(4) it is dried: wet granular is moved into Fast granulate machine dispersion and granulation, then moves in drying and granulating machine dry, until granule dries Dry；

(5) granulate: dry granule is used Fast granulate machine granulate, is dispersed into uniform granule；

(6) tabletting: granule uses sheeting equipment carry out tabletting；

(7) packaging: using automatic packaging machine packaging, case after luggage box of going forward side by side again, warehouse-in preserves.

Embodiment 2

A kind of detoxicating prophylactic treatment liver and compositions of correlated metabolism diseases of protecting the liver, according to the mass fraction, its major ingredient is Radix Puerariae 250 parts, 220 parts of Poria, Herba Taraxaci 60 parts, 50 parts of Radix Glycyrrhizae, Herba Cichorii 180 parts, soybean oligo saccharide 6 parts, soy peptide powder 15 parts, glycine 8 parts, lycopene 3 parts；Its adjuvant is edible corn starch 6 parts, sodium carboxymethyl cellulose 6 parts.

Wherein, Radix Puerariae, Herba Taraxaci are handled as follows:

(1) Radix Puerariae, Herba Taraxaci are cleaned, pulverize respectively after cleaning, coarse powder is crossed 40 mesh aperture sieve；

(2) weighing Radix Puerariae coarse powder and Herba Taraxaci coarse powder, it is the ethanol of 60% that the ratio with solid-liquid ratio as 1:10 adds volumetric concentration Aqueous solution, ultrasonic immersing 8h at a temperature of 60 DEG C, filters；

(3) taking filtering residue, it is the ethanol water of 60% that the ratio with solid-liquid ratio as 1:2 adds volumetric concentration, at a temperature of 60 DEG C Ultrasonic immersing 8h, filters, and twice filtrate is merged, is evaporated to without ethanol；

(4) solution is dialysed, take filtrate and be spray-dried, obtain Radix Puerariae, Herba Taraxaci extract.

Poria, Radix Glycyrrhizae are handled as follows:

(1) Poria, Radix Glycyrrhizae are cleaned, pulverize respectively after cleaning, coarse powder is crossed 40 mesh aperture sieve；

(2) weighing Poria coarse powder and Radix Glycyrrhizae coarse powder, the ratio with solid-liquid ratio as 1:8 adds pure water, heated at constant temperature leaching at 65 DEG C Bubble 30min, heated and boiled 30min, filters；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:2 adds pure water, and at 65 DEG C, heated at constant temperature soaks 30min, and heating is boiled Boiling 30min, filters, and continues to be heated to boiling, and is stirred continuously, make Poria, Radix Glycyrrhizae fluidextract after twice filtrate being merged Standby.

Herba Cichorii is handled as follows:

(1) Herba Cichorii is cleaned, pulverize after cleaning, coarse powder is crossed 40 mesh aperture sieve；

(2) joining in container by Herba Cichorii coarse powder, the ratio with solid-liquid ratio as 1:1 adds 70% ethanol solution, stirs, Moistening airtight standing 2h；

(3) the Herba Cichorii coarse powder after infiltration is transferred in percolator, suitably presses, after dipping 36h, with the speed of 3ml/min Collect extracting solution；

(4) by extracting solution concentrating under reduced pressure, until without ethanol；

(5) concentrate, be dried, obtain Herba Cichorii extract.

The preparation technology of the compositions protecting the liver detoxicating prophylactic treatment liver and correlated metabolism diseases of the present invention, comprises the steps:

(1) weigh: weigh soybean oligo saccharide 6 parts, soy peptide powder 15 parts, glycine 8 parts, lycopene 3 parts, eat Corn starch 6 parts, sodium carboxymethyl cellulose 6 parts, individually deposit；

(2) pelletize: Radix Puerariae and Herba Taraxaci extract, Poria cocos and liquorice fluid extract, Herba Cichorii extract move into efficient wet granulator After middle mix homogeneously, add soy peptide powder, glycine, lycopene, sodium carboxymethyl cellulose, again mix homogeneously, make Granule；

(3) cladding: add soybean oligo saccharide, edible corn starch after step (2) makes granule, by soybean oligo saccharide It is evenly coated at granule appearance with edible corn starch；

(4) it is dried: wet granular is moved into Fast granulate machine dispersion and granulation, then moves in drying and granulating machine dry, until granule dries Dry；

(5) granulate: dry granule is used Fast granulate machine granulate, is dispersed into uniform granule；

(6) tabletting: granule uses sheeting equipment carry out tabletting；

(7) packaging: using automatic packaging machine packaging, case after luggage box of going forward side by side again, warehouse-in preserves.

Embodiment 3

A kind of detoxicating prophylactic treatment liver and compositions of correlated metabolism diseases of protecting the liver, according to the mass fraction, its major ingredient is Radix Puerariae 225 parts, 210 parts of Poria, Herba Taraxaci 55 parts, 45 parts of Radix Glycyrrhizae, Herba Cichorii 170 parts, soybean oligo saccharide 5 parts, soy peptide powder 12 parts, glycine 6 parts, lycopene 3 parts；Its adjuvant is edible corn starch 5 parts, sodium carboxymethyl cellulose 5 parts.

Wherein, Radix Puerariae, Herba Taraxaci are handled as follows:

(1) Radix Puerariae, Herba Taraxaci are cleaned, pulverize respectively after cleaning, coarse powder is crossed 30 mesh aperture sieve；

(2) weighing Radix Puerariae coarse powder and Herba Taraxaci coarse powder, the ratio with solid-liquid ratio as 1:7 adds the ethanol water that volumetric concentration is 50% Solution, ultrasonic immersing 7h at a temperature of 50 DEG C, filters；

(3) taking filtering residue, it is the ethanol water of 50% that the ratio with solid-liquid ratio as 1:1.5 adds volumetric concentration, the temperature of 50 DEG C Lower ultrasonic immersing 7h, filters, and twice filtrate is merged, is evaporated to without ethanol；

(4) solution is dialysed, take filtrate and be spray-dried, obtain Radix Puerariae, Herba Taraxaci extract.

Poria, Radix Glycyrrhizae are handled as follows:

(1) Poria, Radix Glycyrrhizae are cleaned, pulverize respectively after cleaning, coarse powder is crossed 30 mesh aperture sieve；

(2) weighing Poria coarse powder and Radix Glycyrrhizae coarse powder, the ratio with solid-liquid ratio as 1:7 adds pure water, heated at constant temperature leaching at 50 DEG C Bubble 25min, heated and boiled 25min, filters；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:1.5 adds pure water, and at 50 DEG C, heated at constant temperature soaks 25min, heating Boil 25min, filter, continue to be heated to boiling after twice filtrate being merged, and be stirred continuously, make Poria, Radix Glycyrrhizae stream leaching Cream is standby.

Herba Cichorii is handled as follows:

(1) Herba Cichorii is cleaned, pulverize after cleaning, coarse powder is crossed 30 mesh aperture sieve；

(2) joining in container by Herba Cichorii coarse powder, the ratio with solid-liquid ratio as 1:0.9 adds 70% ethanol solution, stirs, Moistening airtight standing 1.5h；

(3) the Herba Cichorii coarse powder after infiltration is transferred in percolator, suitably presses, after dipping 30h, with the speed of 2ml/min Collect extracting solution；

(4) by extracting solution concentrating under reduced pressure, until without ethanol；

(5) concentrate, be dried, obtain Herba Cichorii extract.

The preparation technology of the compositions protecting the liver detoxicating prophylactic treatment liver and correlated metabolism diseases of the present invention, comprises the steps:

(1) weigh: weigh soybean oligo saccharide, soy peptide powder, glycine, lycopene, edible corn starch, carboxymethyl fibre Dimension element sodium, individually deposits；

(2) pelletize: Radix Puerariae and Herba Taraxaci extract, Poria cocos and liquorice fluid extract, Herba Cichorii extract move into efficient wet granulator After middle mix homogeneously, add soy peptide powder, glycine, lycopene, sodium carboxymethyl cellulose, again mix homogeneously, make Granule；

(3) cladding: add soybean oligo saccharide, edible corn starch after step (2) makes granule, by soybean oligo saccharide It is evenly coated at granule appearance with edible corn starch；

(4) it is dried: wet granular is moved into Fast granulate machine dispersion and granulation, then moves in drying and granulating machine dry, until granule dries Dry；

(5) granulate: dry granule is used Fast granulate machine granulate, is dispersed into uniform granule；

(6) tabletting: granule uses sheeting equipment carry out tabletting；

(7) packaging: using automatic packaging machine packaging, case after luggage box of going forward side by side again, warehouse-in preserves.

Carrying out effect experimental with the compositions that prescription described in embodiment 3 and preparation method prepare, specific experiment is as follows.

1, zoopery A

The preparation of experiment reagent: take the compositions that the prescription with embodiment 3 and preparation method prepare, dissolves after pulverizing, joins respectively Make 5,10, the solution of 15mg/ml, now with the current.

Animal packet and model are set up: Normal male mice body weight 18-22g, are divided into 3 groups, and often group 12, is divided into normal control Group, ethanol feed group, embodiment 3 prescriptions+ethanol feed group.Ethanol feed group mice feed every day ethanol 1 time, 5g/kg body Weight；Embodiment 3 prescriptions+ethanol feed group, at feed ethanol first 1 hour, the composition solution of feed 0.2mL variable concentrations, Then feed ethanol 5g/kg；Normal group gives equal-volume (0.2mL) normal saline, continuous gavage 2 weeks.Take blood, point Standby from serum.

Measure GSH content and liver tissue homogenate's lipid peroxide (TBARS) in mitochondrion: put to death by the method for cervical dislocation White mice, cuts rapidly abdominal part open and takes out liver, prepares mitochondrion and liver tissue homogenate measures GSH content and hepatic tissue in mitochondrion Homogenate lipid peroxide (TBARS).The results are shown in Table 1.

The impact of hepatic injury mice ALT, GSH and TBARS that ethanol is induced by table 1 compositions

Note:^※※Compare with Normal group: p ＜ 0.001；^△△Compare with ethanol group: p ＜ 0.001；^△P ＜ 0.05

The above results shows, this prescription has the anti-oxidation protection liver effect of highly significant.After ethanol feed 2 weeks, Mouse Liver line Plastochondria GSH content extremely reduces, and lipid peroxide growing amount (TBARS) significantly raises, and ALT significantly raises, embodiment 3 prescriptions+ethanol feed group feed after 2 weeks mitochondrion GSH similar with Normal group content, TBARS growing amount is slightly above normal Comparison, ALT the most slightly raises.And dosage is the highest, effect is the best.

2, zoopery B

The preparation of experiment reagent: take the compositions that the prescription with embodiment 3 and preparation method prepare, dissolves after pulverizing, joins respectively Make 50,100, the solution of 150mg/ml, now with the current.

Animal packet and model are set up: normal male SD rat, body weight 200-220g, are divided into 4 groups, Normal group, prescription Dosage group, prescription low dose group in high dose group, prescription, filter solution through microfilter before injection, inject 0.2mL every day Solution；Matched group lumbar injection every day equal-volume normal saline 0.2mL, successive administration 14 days.

Measure rat blood serum ALT and TNF-α changes of contents: after 14 days, rat tail vein injects lipopolysaccharide (LPS) 2mg/kg Body weight, takes blood after 120 minutes, separate serum, measures rat blood serum ALT and TNF-α changes of contents.The results are shown in Table 2.

Rat ALT that LPS is induced by table 2 compositions and the impact of TNF-α content

Group	ALT(U/L)	TNF-α(U/mL)
			Normal group	873.7±112.2	7035.2±430.7
High dose group	59.48±7.6※※	516.3±89.5※※
			Middle dosage group	87.4±9.6※	612.5±76.6※
Low dose group	102.31±6.3	806.1±13.9

Note:^※※Compare with matched group: P ＜ 0.001,^※P ＜ 0.05

Above-mentioned test result indicate that, the rat TNF-α that LPS is induced by the present composition has pole to significantly inhibit effect, LPS Can significantly raise control rats ALT, and experimental group ALT the most slightly raises, dosage is the highest simultaneously, and inhibition is the best.Knot Fruit explanation: the hepatic injury of present composition induced by endotoxin induction has extremely strong protective effect.

3, human experimentation

Object of study and packet: from certain city's endocrine diabetes hospital, consulting patients in out-patient department and Physical Examination crowd, choosing Select through color doppler sonography Diagnosis of Fatty patient 862 example (male 558 examples, female 304 example) 52 ± 19 years old mean age (40-71 Year) with reference to " Chinese Medical Association's hepatology branch alcoholic fatty liver and non-alcoholic fatty liver disease diagnostic criteria " by 862 example fat Fat hepatopath is divided into 2 groups: (1) alcoholic fatty liver group (290 example), (2) non-alcoholic fatty liver disease group (572 example).

Observation item: to all fatty liver experimenters (treatment group and matched group) all through the medical speciality technical staff of special training Be responsible for investigating, inquire and record, age, sex, occupation, drink, diabetes, hypertension and history of medications Deng.It is fully completed following physical examination and laboratory and color doppler sonography inspection.

(1) clinical examination

Measure the height (centimetre, cm) of examinee, empty body weight (kilogram, kg) with desk-top balance, during measurement examinee should take off one's shoes, Raise one's hat, unlined garment, unlined trousers measurement.Waistline and hip circumference (centimetre, cm) is measured with tape.The measurement of waistline is with costal margin and crista iliaca Upper intermarginal Xiao Zhou footpath is as the criterion, and through pubic symphysis, both sides through greater trochanter of femur before the measurement of hip circumference, the most prominent by buttocks Going out position is standard, calculates Body Mass Index (BMI)=body weight (Kg)/height (m) 2, waist-to-hipratio (WH)=waistline (cm)/hip circumference (cm).The measuring method of blood pressure (BP) uses National Sampled Study on Hypertension in China unified standard, uses hydrargyrum to lean on formula sphygomanometer without exception. Examinee needs rest 5 minutes of sitting quietly before measuring, then takes seat, measures right upper extremity blood pressure.Shrink pressure (SBP) to hear 1st phase heart sound is as the criterion, and diastolic pressure (DBP) is as the criterion with the 5th phase disappearance sound.It is spaced 2 minutes, measures continuously twice, take Its meansigma methods.The front weighing machine of measurement, sphygomanometer are the most corrected.More than operation is special messenger's special plane and carries out.

(2) lab testing

Full-fledged research object fasting the most overnight 12 hours before treatment, blood drawing on an empty stomach in secondary everyday early morning, employing full-automatic biochemical instrument, Survey fasting glucose (FBG), cholesterol (TC), triglyceride (TG), HDL-C (HDL-C), low close Degree lipoprotein cholesterol (LDL-C), gamma glutamyl transpeptidase (GTT), glutamate pyruvate transaminase (AST), glutamic oxaloacetic transaminase, GOT (ALT), Blood urea nitrogen (BUN), creatinine (Cr), uric acid (UA) etc..Johnson & Johnson of U.S. full-automatic dry chemistry instrument is used to measure tumor necrosis factor (TNF-α), C reactive protein (CRP).

(3) liver color Doppler checks

Liver color ultrasound uses U.S.'s PHILIPS M2540A type color Doppler ultrasound, frequency 10MHz, fixing special messenger's operation. Experimenter's fasting overnight 12 hours, in early morning on the same day, clinostatism of making even, transverse scan combines with vertical disconnected scanning, clearly shows that liver Structure.

Therapeutic Method: all object of study all started to take the present composition the same day, 0.65g/ sheet, 4 pieces/times, 3 times/day, Continuous use 8-10 week.After taking medicine, 4-5 and 8-10 week checks These parameters and lab testing respectively, observes and notes down patient To drug reaction etc..

Observation index and the standard of curative effect evaluation: extract limosis vein blood before and after treatment respectively, measure fasting glucose (FBG), gallbladder solid Alcohol (TC), triglyceride (TG), HDL-C (HDL-C), low-density lipoprotein cholesterol (LDL-C), Gamma glutamyl transpeptidase (GTT), glutamate pyruvate transaminase (AST), glutamic oxaloacetic transaminase, GOT (ALT), blood urea nitrogen (BUN), creatinine (Cr), Uric acid (UA), tumor necrosis factor (TNF-α), C reactive protein (CRP), measure blood pressure, and row liver color Doppler checks, Being carried out by same position doctor before and after treatment, paired observation ultrasonography of liver changes.

(1) curing: symptom, sign disappear substantially, liver function indices recovers normal, and super liver ultrasonogram recovers normal.

(2) effective: symptom, sign are obviously improved, liver function recovery is normal or declines more than 50%, and ultrasonic liver picture figure is obvious Improve (declining 1 grade), the indices at least 1 such as blood fat, uric acid (UA), tumor necrosis factor (TNF-α) More than recover normal or decline more than 50%；

(3) effective: to cure and effective person is all judged to effectively；

(4) invalid: symptom, sign are not improved, liver function, blood fat indices decline less than 20%, B ultrasonic liver acoustic image Figure is without significant change.

Interpretation of result and statistical procedures: statistical analysis uses spss11.5 software kit to carry out, group difference uses t inspection, Multiple sample averages compare uses variance analysis.Every result see table.

Table 3 compositions general Index Influence clinical to Patients with Fatty Liver

Project	Before treatment	After treatment	P value
				Number of cases (male/female)	862(558/304)	862(558/304)
Age	52.10±12.19	52.10±12.19
				Height (m)	1.68±0.87	1.68±0.87
Body weight (Kg)	78.38±14.09	78.05±13.28	0.56
				Body Mass Index (BMI)	27.75±3.51	27.65±3.27	0.87
Waistline (cm)	94.86±7.73	94.51±6.88	0.70
				Hip circumference (cm)	101.28±5.57	100.95±5.38	0.94
Waist/buttocks	0.94±0.06	0.94±0.05	0.825
				Shrink pressure (mmHg)	130.31±15.56	129.60±14.86	0.410
Diastolic pressure (mmHg)	83.73±7.60	83.15±7.42	0.447
				Body fat content (%)	34.52±3.83	32.45±3.47	0.56

The above results shows, after treatment, the body weight of patient, BMI, waistline, hip circumference have declined before relatively treating, but statistics Learn difference not notable, and body fat content is decreased obviously.

The impact on Patients with Fatty Liver metabolism of table 4 compositions

Project	Before treatment	After treatment	P value
				FBG(mmol/L)	6.35±2.02	6.24±1.71	0.583
TC(mmol/L)	5.77±1.41	5.36±1.54	0.066
				TG(mmol/L)	2.62±2.27	1.37±0.61	0.000*
LDL(mmol/L)	3.45±1.63	3.51±1.48	0.38
				HDL(mmol/L)	1.13±0.48	1.22±0.46	0.365

The impact on Patients with Fatty Liver liver function of table 5 compositions

Project	Before treatment	After treatment	P value
				GGT(U/L)	54.66±21.40	34.40±9.81	0.000*
ALT(U/L)	54.53±24.05	33.19±10.33	0.000*
				AST(U/L)	51.08±27.72	26.15±9.32	0.000*

The impact on Patients with Fatty Liver renal function of table 6 compositions

Project	Before treatment	After treatment	P value
				BUN(mmol/L)	5.19±1.51	5.17±1.25	0.620
Cr(umol/L)	74.80±13.88	75.45±17.10	0.352
				UA(umol/L)	364.2±86.18	358.1±62.62	0.368

The impact on inflammatory cytokine special on Patients with Fatty Liver of table 7 compositions

Project	Before treatment	After treatment	P value
				TNF(pg/ml)	17.82±6.84	9.58±3.17	0.000*
CRP(mg/L)	3.12±0.91	2.42±0.68	0.000*

From above-mentioned serial experiment, the present composition can significantly reduce the inflammation such as ALT, AST level and suppression TNF-α Sexual cell factor effect, to improving and reverse liver function and intracellular antioxidation and anti-inflammatory effect is very notable, it addition, The present composition still has reduction body fat content, TC, TG, adjusts and improve metabolism.

Claims (5)

1. one kind protects the liver detoxicating prophylactic treatment liver and the compositions of correlated metabolism diseases, it is characterised in that: according to the mass fraction, its Major ingredient be Radix Puerariae 200-250 part, Poria 200-220 part, Herba Taraxaci 50-60 part, Radix Glycyrrhizae 40-50 part, Herba Cichorii 160-180 part, Soybean oligo saccharide 5-6 part, soy peptide powder 10-15 part, glycine 5-8 part, lycopene 2-3 part；Its adjuvant is edible corn Starch 5-6 part, sodium carboxymethyl cellulose 4-6 part.

The most according to claim 1 protect the liver detoxicating prophylactic treatment liver and the compositions of correlated metabolism diseases, it is characterised in that: Described Radix Puerariae, Herba Taraxaci are handled as follows:

(1) Radix Puerariae, Herba Taraxaci are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) the Radix Puerariae coarse powder of 200-250 mass fraction and the Herba Taraxaci coarse powder of 50-60 mass fraction are weighed, with solid-liquid ratio as 1:5-10 Ratio add volumetric concentration be the ethanol water of 40-60%, ultrasonic immersing 6-8h at a temperature of 40-60 DEG C, filter；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:1-2 adds the volumetric concentration ethanol water as 40-60%, the temperature of 40-60 DEG C The lower ultrasonic immersing 6-8h of degree, filters, twice filtrate is merged, be evaporated to without ethanol；

(4) solution is dialysed, take filtrate and be spray-dried, obtain Radix Puerariae, Herba Taraxaci extract.

The most according to claim 1 protect the liver detoxicating prophylactic treatment liver and the compositions of correlated metabolism diseases, it is characterised in that: Described Poria, Radix Glycyrrhizae are handled as follows:

(1) Poria, Radix Glycyrrhizae are cleaned, pulverize respectively after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) the Poria coarse powder of 200-220 mass parts and the Radix Glycyrrhizae coarse powder of 40-50 mass fraction, the ratio with solid-liquid ratio as 1:5-8 are weighed Example adds pure water, and at 45-65 DEG C, heated at constant temperature soaks 20-30min, heated and boiled 20-30min, filters；

(3) taking filtering residue, the ratio with solid-liquid ratio as 1:1-2 adds pure water, and at 45-65 DEG C, heated at constant temperature soaks 20-30min, heating Boil 20-30min, filter, continue to be heated to boiling after twice filtrate being merged, and be stirred continuously, make Poria, Radix Glycyrrhizae stream Extractum is standby.

The most according to claim 1 protect the liver detoxicating prophylactic treatment liver and the compositions of correlated metabolism diseases, it is characterised in that: Described Herba Cichorii is handled as follows:

(1) Herba Cichorii is cleaned, pulverize after cleaning, coarse powder is crossed 20-40 mesh aperture sieve；

(2) joining in container by Herba Cichorii coarse powder, the ratio with solid-liquid ratio as 1:0.8-1 adds 70% ethanol solution, stirs, Moistening airtight standing 1-2h；

(3) the Herba Cichorii coarse powder after infiltration is transferred in percolator, suitably presses, after dipping 24-36h, with the speed of 1-3ml/min Collect extracting solution；

(4) by extracting solution concentrating under reduced pressure, until without ethanol；

(5) concentrate, be dried, obtain Herba Cichorii extract.

The preparation technology of the compositions protecting the liver detoxicating prophylactic treatment liver and correlated metabolism diseases the most according to claim 1, its It is characterised by: comprise the steps:

(1) weigh: weigh soybean oligo saccharide 5-6 part, soy peptide powder 10-15 part, glycine 5-8 part, lycopene 2-3 part, Edible corn starch 5-6 part, sodium carboxymethyl cellulose 4-6 part, individually deposit；

(2) pelletize: Radix Puerariae and Herba Taraxaci extract, Poria cocos and liquorice fluid extract, Herba Cichorii extract move in efficient wet granulator mixed After closing uniformly, add soy peptide powder, glycine, lycopene, sodium carboxymethyl cellulose, again mix homogeneously, make granule；

(3) cladding: add soybean oligo saccharide, edible corn starch after step (2) makes granule, by soybean oligo saccharide and food It is evenly coated at granule appearance with corn starch；

(4) it is dried: wet granular is moved into Fast granulate machine dispersion and granulation, then moves in drying and granulating machine dry, until granule is dried；

(5) granulate: dry granule is used Fast granulate machine granulate, is dispersed into uniform granule；

(6) tabletting: granule uses sheeting equipment carry out tabletting；

(7) packaging: using automatic packaging machine packaging, case after luggage box of going forward side by side again, warehouse-in preserves.