CN103766904A - Preparation of capsule with function of lowering lipid and facilitating feces excretion and application of capsule - Google Patents

Preparation of capsule with function of lowering lipid and facilitating feces excretion and application of capsule Download PDF

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Publication number
CN103766904A
CN103766904A CN201410029675.9A CN201410029675A CN103766904A CN 103766904 A CN103766904 A CN 103766904A CN 201410029675 A CN201410029675 A CN 201410029675A CN 103766904 A CN103766904 A CN 103766904A
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natto
capsule
asparagus
parts
powder
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Chinese (zh)
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陈斌
和七一
何正波
陈治霖
郝友进
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Chongqing Normal University
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Chongqing Normal University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/212—Starch; Modified starch; Starch derivatives, e.g. esters or ethers
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material

Abstract

The invention discloses a capsule with function of lowering lipid and facilitating feces excretion and a preparation method of the capsule. The capsule comprises the following raw medicines in parts by weight: 40-50 parts of natto powder, 35-40 parts of dietary fiber, 5-10 parts of a ginkgol biloba extract, 10-15 parts of pueraria root powder and pharmaceutically acceptable auxiliary materials. The capsule product is good in nutrition and healthcare function, and simple and convenient to take; the freeze-dried natto retains nutrition and function components of the natto to the greatest extent; the product has the effects of preventing and treating cardiovascular and cerebrovascular diseases, changing human intestinal flora ecology, facilitating digestion, relieving constipation, lowering lipid, reducing pressure, decreasing internal heat, clearing heat and removing toxicity; the product also takes the special effects on beauty fitness, breast augmentation, and the like of women; the influence of the natto, the pueraria root powder and asparagus on taste of consumers can be avoided.

Description

A kind of preparation and application thereof with lipopenicillinase, bowel relaxing functions capsule
Technical field
The invention belongs to health products (healthy food) manufacture field, relate to a kind of preparation and application thereof with lipopenicillinase, bowel relaxing functions capsule and use.
Background technology
Natto is a kind of fermented food that utilizes Bacillus natto to obtain in soybean top fermentation, is deeply subject to liking of japanese people in Japan.Fermented soya bean with China are the same, and natto has had the history of more than one thousand years.Within 1987, the Japanese physiology professor doctor of Xu Jian foreign firm finds Nattokinase from natto, and confirms that Nattokinase has after good thrombus effect, and natto gains considerable fame especially.Nattokinase is a kind of serine protease that Bacillus natto produces in the process of fermentation, research shows, Nattokinase can effectively be removed the thrombus in blood of human body, and hypertension, aging, apoplexy, muscle cramp and cardiovascular and cerebrovascular disease are also had to good curative effect, and have no side effect, Bacillus natto also produces a lot of other materials as vitamin K, protease, polypeptide etc. in the process of fermenting simultaneously, these materials are all conducive to health, and therefore Nattokinase is developed to middle-aged and old health foods by numerous companies.
Bacillus natto is subspecies for bacillus subtilis; it is a kind of beneficial bacterium acidproof, heat-resistant quality that has; under hydrochloric acid in gastric juice, four hours survival rates are 100%, have powerful pathogen and suppress ability, self do not have pathogenic simultaneously; in the middle of various beneficial bacterium; to environment tolerance one of the bacterial classification of small intestine that preferably can go directly, can change the human enteric bacteria state of growing thickly after oral, help digest function normalization; so that defecation is smooth and easy, maintain body physiological environmental protection.
Dietary fiber is that polysaccharide and the wood that can not be decomposed by human body alimentary canal ferment are planted element, has the effect that absorbs moisture content in digestive system.Dietary fiber can increase the food volume in enteron aisle and stomach, can increase satiety; Enterogastric peristalsis can be promoted again, constipation can be alleviated; Dietary fiber also can adsorb the harmful substance in enteron aisle to discharge simultaneously.Asparagus has another name called asparagus, is rich in several amino acids, protein and vitamin, and its content all, higher than general fruits and vegetables, is enjoyed the laudatory title of " kings of vegetables " in the international market.Along with growing to asparagus demand of people, the processing of asparagus leftover bits and pieces also becomes a large problem.From asparagus leftover bits and pieces, extract and prepare dietary fiber, not only can be salvaged, improve asparagus added value, can also avoid asparagus leftover bits and pieces to huddle and leave about the problem of environmental pollution causing.
Ginkgo biloba p.e be leaf take ginkgo as raw material, adopt suitable solvent, a series products of the active ingredient enrichment of extraction.The medical value of ginkgo biloba p.e is very extensive with application.Ginkgo biloba p.e can promote the circulation of brain and health limbs simultaneously.An its main health care suppresses the material that one is called platelet activating factor (PAF) exactly, and PAF is a kind of medium discharging from cell, and it can cause platelet aggregation (being deposited in together).The PAF of high-load can cause neural cell injury, and central nervous system CBF reduces, inflammation, and bronchoconstriction.Closely similar with free radical, high PAF level also can cause aging.Ginkgolides and Bilobalide can protect the nerve cell of central nervous system injury-free in period at ischemic (in-vivo tissue lacks oxygen).This function may be able to have to suffering from the patient of apoplexy the effect of supplemental treatment.Except suppressing thrombocyte adhesiveness, ginkgo biloba extract regulates antiotasis and elastic force.It can make blood vessel circulation more efficient.This lifting cycle efficieny effect has same purpose to the trunk in the circulatory system (artery) and less blood vessel (capillary).
Kudzu-vine root powder is the starch extracting from Wild Pueraria, there is lipopenicillinase, step-down, fall fire, clearing heat and detoxicating, rise positive expelling pathogenic factors from muscles and skin, spasmolysis and analgesia, promoting eruption antidiarrheal, increase brain and crown blood sugar, improve the effects such as brain microcirculation, it is the important source material of preventing and treating the medicine of the various diseases such as hypertension, high fat of blood, coronary heart diseases and angina pectoris, diabetes, cerebral thrombus, enterogastritis, women's cosmetology and health, chest enlarge etc. are had to special efficacy, be the wholefood that nutritive value is very high simultaneously.
In recent years, the processing of natto product has development faster, and Chewable Tablets, natto drink powder, Notto beverage, natto yoghourt are come out one after another.Further investigation reveals that, these products have certain nutritive value, but market reaction is flat, traces it to its cause, and be mainly that the natto local flavor that Japan produces is difficult to Chinese's acceptance, and independent natto powder effect are limited.The present invention has gone out to have the natto dietary fiber capsule product of lipopenicillinase, ease constipation defecation with natto powder mixed developing with kudzu-vine root powder, ginkgo biloba extract, dietary fiber.
Summary of the invention
The object of the present invention is to provide and a kind ofly contain wholefood that abundant nutrition is worth as raw material take multiple, a kind of capsule with health care obtaining through the preparation of scientific and reasonable technique and proportioning and preparation method thereof.In order to realize object of the present invention, intend adopting following technical scheme:
One aspect of the present invention relates to a kind of capsule with health care, it is characterized in that described capsule comprises the bulk drug of following weight portion:
40～50 parts of natto powders, 35～40 parts of dietary fibers, 5～10 parts of ginkgo biloba extracts, 10～15 parts of kudzu-vine root powder;
And pharmaceutically acceptable auxiliary material; Preferably, described ginkgo biloba extract is dry ginnol extract.
In a preferred embodiment of the present invention, described dietary fiber is the dietary fiber that asparagus or asparagus leftover bits and pieces prepare.
In a preferred embodiment of the present invention, described dietary fiber prepares as follows: by the processing of asparagus leftover bits and pieces drying, cross 40 mesh sieves after ultramicro grinding, make asparagus powder;
Take 100 weight portion asparagus powders, add 800-1200 weight portion purified water, stir; After 5-7 weight portion lipase is fully dissolved by purified water, adding in above-mentioned asparagus powder, is to act on 1.5-2.5h under 9.0～9.5,35 ℃ of conditions in pH value, and enzyme goes out;
Asparagus liquid after above-mentioned lipase enzymolysis is cooled to 40～45 ℃, and adjusting pH value is 6.0～6.5, adds 12-18 weight portion amylase, and after 45 ℃ of effect 3-5h, enzyme goes out;
Asparagus liquid after lipase and amylase enzymolysis is cooled to 40～45 ℃, and adjust pH 7.0～7.5, adds 12-18 weight portion protease effect 0.8-1.2h, enzyme goes out, then filter with four layers of gauze, after filter residue water cleans, dry and obtain dietary fiber.
In another preferred embodiment of the present invention, described natto powder prepares as follows:
The making of freeze dry powder of fermented soybean: choose full grains, soya bean of uniform size, after soaking, autoclave sterilization pot 25-35min processes, and natto subject to sterilization is cooled to 40 ℃ of appropriate Bacillus natto seed liquor of following rear inoculation under suitable temperature and humidity, to cultivate 20-28h and obtains fresh natto; 200 mesh sieves were pulverized in the natto freeze drying fermenting, and obtained freeze-dried powder.
In a preferred embodiment of the present invention, described Bacillus natto seed liquor is that deposit number is the Bacillus natto seed liquor of CGMCCNO.7168.
The present invention also relates to above-mentioned capsule on the other hand in the purposes of preparing in medicine or health products, described medicine or health products for cardiovascular and cerebrovascular disease, change human enteric bacteria grow thickly state, alleviate constipation, lipopenicillinase, step-down, fall fire, clearing heat and detoxicating and/or chest enlarge.
The natto powder that the present invention uses can make by the present invention's processing; Dietary fiber used can be by preparing in asparagus or asparagus leftover bits and pieces; Ginkgo biloba p.e, kudzu-vine root powder can be bought and obtain on market.Bacillus natto HT8 deposit number is that this bacterial strain of CGMCCNO.7168(has been documented in Chinese patent application 201310161221.2,201310681770.2,201310681809.0 in), be preserved in common micro-organisms culture presevation administrative center of China Committee for Culture Collection of Microorganisms.
Technical scheme of the present invention:
1. the making of freeze dry powder of fermented soybean: choose full grains, soya bean of uniform size, after soaking, autoclave sterilization pot 30min processes, and natto subject to sterilization is cooled to 40 ℃ of appropriate Bacillus natto seed liquor of following rear inoculation under suitable temperature and humidity, to cultivate 24h and obtains fresh natto.200 mesh sieves were pulverized in the natto freeze drying fermenting, and obtained freeze-dried powder.
2. the preparation of dietary fiber: by asparagus or its leftover bits and pieces drying processing, cross 40 mesh sieves after ultramicro grinding, make asparagus powder.Asparagus powder is by four layers of filtered through gauze after lipase, amylase, protease hydrolyzed digestion, and filter residue cleaning, drying obtains dietary fiber.
3. press 40～50 parts of natto powders, 35～40 parts of dietary fibers, 5～10 parts of ginkgo biloba extracts, the weight ratio that kudzu-vine root powder is 10～15 parts mixes dress capsule, the filling 250mg of every capsules.
The present invention has following beneficial effect:
The healthy food that these four kinds of natto powder, dietary fiber, ginkgo biloba extract and kudzu-vine root powder are had better nutritivity health care value by the present invention is fused together, produce a kind of capsule product that abundant nutrition is worth that has, there is good nutrition and health care effect, and take simple and conveniently, frozen dried natto has retained nutrition and the functional component of natto to the full extent; Product has control cardiovascular and cerebrovascular disease, change the human enteric bacteria state of growing thickly, help digest, alleviate constipation, lipopenicillinase, step-down, fall fire, the effect such as clearing heat and detoxicating, women's cosmetology and health, chest enlarge etc. are had to special efficacy simultaneously, and avoided natto, kudzu-vine root powder, the impact of asparagus on consumer taste.
Microorganism information
The Bacillus natto HT8 bacillus subtilis (Bacillus subtilis) using in a preferred embodiment of the present invention and the specific embodiment has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCCNO.7168, preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation date is 2013.1.18.
The specific embodiment
Illustrate in greater detail embodiment of the present invention below in conjunction with subordinate list and embodiment.But enforcement of the present invention is not limited to the following stated, any pro forma accommodation that the present invention is made and/or change all will fall into protection domain of the present invention.
In the present invention, if not refer in particular to, all equipment and raw materials etc. all can be buied from market or the industry is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
Embodiment 1:
The preparation of freeze dry powder of fermented soybean:
1. the processing of soya bean: choose full grains, soya bean of uniform size, mixes with the ratio of water 1:3 in soya bean, soaks 3h at 30 ℃, drains, then take a certain amount of in small beaker, under autoclave sterilization pot, 121 ℃ of sterilizing 30min, cooling, stand-by.
2. actication of culture: draw 100ul preservation Bacillus natto bacterial classification to 10mlLB culture medium, 37 ℃, 200r/min cultivates 12h, for subsequent use in Refrigerator store.
3. the preparation of seed liquor: get the activation HT8 bacterial classification of 200ul to 20ml liquid seed culture medium, 37 ℃, 200r/min cultivates 18h.
4. inoculated and cultured: natto subject to sterilization is cooled to, below 40 ℃, HT8 is inoculated in soya bean with 4% inoculum concentration, in 37 ℃, cultivates 24h under 90% relative humidity.
Freeze-dried powder make: first by the natto fermenting in-20 ℃ of pre-freezes, in-60 ℃ of vacuum freeze drying 24h, then frozen dried natto was pulverized to 200 mesh sieves, obtain freeze-dried powder.
The preparation of dietary fiber:
1. by the processing of asparagus leftover bits and pieces drying, after ultramicro grinding, cross 40 mesh sieves, make asparagus powder.
2. take 100g asparagus powder, add 1000ml purified water, stir.After 6g lipase is fully dissolved by a small amount of purified water, adding in above-mentioned asparagus powder, is to act on 2h under 9.0～9.5,35 ℃ of conditions in pH value, and enzyme goes out;
3. the asparagus liquid after above-mentioned lipase enzymolysis is cooled to 40～45 ℃, adjusting pH value is 6.0～6.5, adds 15g amylase, and after 45 ℃ of effect 4h, enzyme goes out;
4. the asparagus liquid after lipase and amylase enzymolysis is cooled to 40～45 ℃, adjust pH 7.0～7.5, adds 15g protease effect 1h, and then the enzyme that goes out filters with four layers of gauze, after filter residue water cleans, dries and obtain dietary fiber.
5. according to following quality proportioning: 50 parts of natto powders, 35 parts of dietary fibers, 5 parts of ginkgo ethanol extracts, 10 parts of kudzu-vine root powder.Filling capsule after mixing, every 250mg.
Embodiment 2:
Prepare natto powder and dietary fiber according to method in embodiment 1, mix a component by following constituent mass proportioning: 45 parts of natto powders, 40 parts of reed food fibers, 5 parts of ginkgo biloba extracts, 10 parts of kudzu-vine root powder, filling capsule, every 250mg.
Embodiment 3:
Prepare natto powder and dietary fiber according to method in embodiment 1, mix a component by following constituent mass proportioning: 45 parts of natto powders, 35 parts of dietary fibers, 5 parts of ginkgo biloba extracts, 15 parts of kudzu-vine root powder, filling capsule, every 250mg.
Pharmacodynamic experiment embodiment 1:
Natto dietary fiber Capsule in Rats regulating blood lipid action:
Natto dietary fiber capsule formula used is each constituent mass proportioning used in embodiment 1.According to " function of health food is learned assessment process and the method for inspection " 2003 auxiliary adjustment of blood fat methods.Select 50 of clean level Wistar kind male rats, body weight 200～250g, afterbody is got the content of blood survey serum total cholesterol (TC) and triglycerides (TG), be divided at random 5 groups according to TC level, be basic, normal, high 3 dosage groups, negative control group and the high fat control group of tested material.Except negative control group, other each group all gives high lipid food and feeds.The dosage of 3 dosage groups is respectively 165mg/kg, 495mg/kg, 990mg/kg (being respectively 5 times, 15 times, 30 times amounts of human body RD), all give tested material by 1ml/100g per os every day gavage, negative control group and high fat control group give same volume pure water.After gavage 30d, put to death animal continuously, measure serum TC, TG index.
Result is as shown in table 1, and natto dietary fiber capsule has the effect that reduces TC and TG in rat blood serum.With the comparison of high fat control group, to take TC content in the rear rat blood serum of low dosage capsule (165mg/kg) and significantly reduce (P<0.05), TG content extremely significantly reduces (P<0.01).
The impact of table 1 natto dietary fiber Capsule in Rats blood fat (mmol/L,
Figure BDA0000460448620000051
)
Figure BDA0000460448620000052
Compared with negative control group, #p<0.05, ##p<0.01; Compared with high fat control group, *p<0.05, *p<0.01.
Pharmacodynamic experiment embodiment 2:
The effect of natto dietary fiber capsule to mouse defecation:
Natto dietary fiber capsule formula used is each constituent mass proportioning used in embodiment 1.Get fasting, can't help 50 of water 12h adult mices, be divided at random 5 groups, be made as physiological saline control group, small dose group (165mg/kg), middle dosage group (485mg/kg), heavy dose of group (990mg/kg) and positive controls (2.5mg/ml phenolphthalein solution), every group 10, physiological saline and medicine all configure with 50% burnt black ink.Mouse was put into metabolic cage in after administration 30 minutes, observed and recorded mouse is arranged the time of melena for the first time, and stool in mice of collection per hour, and 5h continuously calculates the grain number of stool in mice in 5h, and records the shape of ight soil.
The impact of table 2 natto dietary fiber capsule on mouse defecation
Figure BDA0000460448620000061
Figure BDA0000460448620000062
Note: with the comparison of physiological saline group, * p<0.05, * * p<0.01.
Result is as shown in table 2, and natto dietary fiber capsule can accelerate mouse defecation, shortens the time of first grain row melena.With the comparison of physiological saline group, first grain row melena time shorten is the heavy dose of group of 92.4min(), there is significant difference (p<0.05); In unit interval, the number of times of mouse defecation also obviously increases, and the water content of ight soil also obviously increases.
Illustrate: the dietary fiber in the present invention can derive from the leftover bits and pieces producing in other plant of being rich in dietary fiber or its process.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited to this, and any variation of expecting without creative work or replacement, within all should being encompassed in protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain that claims were limited.

Claims (6)

1. there is a capsule for health care, it is characterized in that described capsule comprises the bulk drug of following weight portion:
40～50 parts of natto powders, 35～40 parts of dietary fibers, 5～10 parts of ginkgo biloba extracts, 10～15 parts of kudzu-vine root powder;
And pharmaceutically acceptable auxiliary material; Preferably, described ginkgo biloba extract is dry ginnol extract.
2. capsule according to claim 1, described dietary fiber is the dietary fiber that asparagus or asparagus leftover bits and pieces prepare.
3. capsule according to claim 2, described dietary fiber prepares as follows: by the processing of asparagus leftover bits and pieces drying, cross 40 mesh sieves after ultramicro grinding, make asparagus powder;
Take 100 weight portion asparagus powders, add 800-1200 weight portion purified water, stir; After 5-7 weight portion lipase is fully dissolved by purified water, adding in above-mentioned asparagus powder, is to act on 1.5-2.5h under 9.0～9.5,35 ℃ of conditions in pH value, and enzyme goes out;
Asparagus liquid after above-mentioned lipase enzymolysis is cooled to 40～45 ℃, and adjusting pH value is 6.0～6.5, adds 12-18 weight portion amylase, and after 45 ℃ of effect 3-5h, enzyme goes out;
Asparagus liquid after lipase and amylase enzymolysis is cooled to 40～45 ℃, and adjust pH 7.0～7.5, adds 12-18 weight portion protease effect 0.8-1.2h, enzyme goes out, then filter with four layers of gauze, after filter residue water cleans, dry and obtain dietary fiber.
4. according to the capsule described in claim 1-3 any one, described natto powder prepares as follows:
The making of freeze dry powder of fermented soybean: choose full grains, soya bean of uniform size, after soaking, autoclave sterilization pot 25-35min processes, and natto subject to sterilization is cooled to 40 ℃ of appropriate Bacillus natto seed liquor of following rear inoculation under suitable temperature and humidity, to cultivate 20-28h and obtains fresh natto; 200 mesh sieves were pulverized in the natto freeze drying fermenting, and obtained freeze-dried powder.
5. capsule according to claim 4, described Bacillus natto seed liquor is that deposit number is the Bacillus natto seed liquor of CGMCCNO.7168.
6. the capsule described in claim 1-5 any one is in the purposes of preparing in medicine or health products, described medicine or health products for cardiovascular and cerebrovascular disease, change human enteric bacteria grow thickly state, alleviate constipation, lipopenicillinase, step-down, fall fire, clearing heat and detoxicating and/or chest enlarge.
CN201410029675.9A 2014-01-22 2014-01-22 Preparation of capsule with function of lowering lipid and facilitating feces excretion and application of capsule Pending CN103766904A (en)

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CN105124445A (en) * 2015-09-28 2015-12-09 陈薇 Detoxifying and fire purging natto powder and preparing method
CN105456050A (en) * 2015-12-31 2016-04-06 青岛美博生物技术有限公司 Freeze-dried powder, essence and skincare set for repairing acne marks and scars
CN105661335A (en) * 2016-02-25 2016-06-15 贵州秀生堂医药生物有限公司 Preparation method of natto enteric capsule functional food
CN107041865A (en) * 2016-02-05 2017-08-15 重庆井谷元食品科技有限公司 A kind of natto particle with beauty functions and preparation method thereof
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CN110584097A (en) * 2019-09-23 2019-12-20 黑龙江八一农垦大学 Meal replacement powder and preparation method and application thereof
CN112753923A (en) * 2021-01-19 2021-05-07 中华全国供销合作总社济南果品研究院 Peony pistil ultrafine powder solid beverage and preparation method thereof
CN114403388A (en) * 2022-01-19 2022-04-29 安徽工程大学 Double-strain fermentation functional flavor type dried bamboo shoots and preparation method thereof

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CN104256583A (en) * 2014-09-05 2015-01-07 洛阳华以生物工程有限公司 Formula of health-care product adopting natto freeze-dried powder and ginkgo biloba extract as main materials
CN105124445A (en) * 2015-09-28 2015-12-09 陈薇 Detoxifying and fire purging natto powder and preparing method
CN105456050A (en) * 2015-12-31 2016-04-06 青岛美博生物技术有限公司 Freeze-dried powder, essence and skincare set for repairing acne marks and scars
CN107041865A (en) * 2016-02-05 2017-08-15 重庆井谷元食品科技有限公司 A kind of natto particle with beauty functions and preparation method thereof
CN105661335A (en) * 2016-02-25 2016-06-15 贵州秀生堂医药生物有限公司 Preparation method of natto enteric capsule functional food
CN107997157A (en) * 2017-12-01 2018-05-08 宋波 Quick-freezing type food containing Nattokinase
CN110584097A (en) * 2019-09-23 2019-12-20 黑龙江八一农垦大学 Meal replacement powder and preparation method and application thereof
CN112753923A (en) * 2021-01-19 2021-05-07 中华全国供销合作总社济南果品研究院 Peony pistil ultrafine powder solid beverage and preparation method thereof
CN112753923B (en) * 2021-01-19 2022-11-22 中华全国供销合作总社济南果品研究院 Peony pistil superfine powder solid beverage and preparation method thereof
CN114403388A (en) * 2022-01-19 2022-04-29 安徽工程大学 Double-strain fermentation functional flavor type dried bamboo shoots and preparation method thereof
CN114403388B (en) * 2022-01-19 2023-09-19 安徽工程大学 Double-strain fermentation functional flavor dried bamboo shoots and preparation method thereof

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